FvH4_5g36810

Phospho-2-dehydro-3-deoxyheptonate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015832
Physical Location & Seq
Reverse (-)
26976953 .. 26981390
4438 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g36810.t1

Sequence Viewer

Length: 1605 bp
ATGGCTCTTACTTCTACCAGTTCAATCATTTCGGCAAAATCTTCATTCTTGTCCTCCGGAACTCACCAACCTTCTCTTCCCGGCAGACCCAGCATCGTCGCCGTCCACGCAGCCGACCCCGCCAAGAACCCAGCTGTGTCGACGAAGCAGCAGACGGTGACGCCGGTGCCAGCGTCACCGTCAACTGCGGCGTCTAATGGCTCCACGACAAGCCGGAATGCGGTGCCGGCGAAATGGAGCGTGGACTCGTGGAAATCCAAGAAGGCGCTGCAATTGCCGGAGTACCCGAATAAGGAGGATCTGGAGTCGGTGCTCCAGACGCTGGACTCCTTCCCGCCGATTGTGTTCGCCGGCGAGGCTCGGAGCCTAGAGGAGCGTCTGGCTGAGGCCGCCATGGGAAACGCGTTTCTTCTTCAGGGCGGGGATTGTGCGGAGAGCTTCAAGGAGTTCAATGCCAACAATATTCGTGACACTTTCAGGATTCTGCTCCAGATGGGCGCTGTTTTGATGTTCGGAGGCCAAATGCCAGTAATTAAGGTGGGAAGGATGGCGGGTCAGTTTGCGAAGCCGAGATCCGACCCGTTTGAGGAGAAGGACGGTGTCAAGCTGCCGAGTTACAGAGGAGACAATGTGAACGGAGACGCGTTTGATTCGAAGTCAAGGACTCCGGACCCTGAGAGATTGATCAGAGCCTACTGTCAATCTGCGGCGACTCTCAATCTTCTCAGGGCTTTCGCAACTGGTGGGTATGCGGCTATGCAGAGGGTCACCCAGTGGAACTTGGATTTCACAGAGCAGAGCGAACAGGGAGATAGGTACCGGGAACTTGCTCACCGGGTTGATGAGGCTCTTGGATTCATGACTGCAGCTGGACTCACAGTTGACCATCCTATTATGACAACCACCGAGTTCTGGACATCCCATGAGTGCTTGCTCTTGCCTTATGAGCAGTCGCTCACTAGGTTGGACTCAACTTCTGGGCTCTACTATGATTGCTCTGCCCATATGCTTTGGGTTGGTGAGCGAACCAGGCAACTGGATGGCGCCCATATTGAGTTCCTTAGAGGTGTTGCTAACCCCCTTGGAATCAAGGTGAGTGATAAGATGGATCCCAATGAGCTTGTTAAGCTTATAGAGATTTTGAATCCTCAAAATAAAGCCGGGAGGATAACAATAATCACTAGAATGGGAGCTGAGAACATGAGAGTGAAACTTCCTCATCTTATAAGAGCAGTCCGTAGGGCAGGACAGATTGTCACATGGGTCAGCGACCCTATGCATGGAAACACCATCAAGGCTCCATGTGGTCTCAAGACACGCCCTTTTGATGCCATAATGGCCGAGGTCAGAGCATTTTTCGATGTCCACGACCAAGAAGGAAGCCATCCAGGAGGTGTGCATCTTGAGATGACAGGCCAAAATGTGACCGAATGCATAGGAGGGTCACGGACTGTGACATTTGACGACCTCAGCTCGAGATATCATACGCATTGTGACCCCAGGCTAAATGCCTCCCAATCTTTGGAGCTTGCTTTTATTATCGCCGAGCGCCTAAGGAGACGAAGGATCAAGTCCCAACTATCTGTTTCCTCATTAGGTCTATAA

Protein Analysis

535

Amino Acids

58.69

Weight (kDa)

7.68

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAHP_synth_2 PF01474 79 - 515 1.9e-205 Class-II DAHP synthetase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22410
fragaria_vesca FvH4_5g36810
malus_domestica MD08G1186700.v1.1 MD15G1372600.v1.1
prunus_persica Prupe.1G521200_v2.0.a1
pyrus_communis pycom08g16050 pycom15g33360
rosa_chinensis RchiOBHm_Chr7g0243371
rosa_laevigata RLG00000000852
rosa_multiflora Rmu_sc0001036.1_g000028 Rmu_sc0001461.1_g000106
rosa_roxburghii Rroxscaffold_3G00222150
rosa_rugosa Rorug07G0318600
rosa_samantha Rh7BG444800
rosa_wichuraiana Rw7G039490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1226
Acc65I GGTACC 1 cut(s) 816
AccB1I GGYRCC 4 cut(s) 166, 223, 816, 1043
AccB7I CCANNNNNTGG 2 cut(s) 322, 1522
AccI GTMKAC 1 cut(s) 140
AccII CGCG 2 cut(s) 404, 644
AccIII TCCGGA 2 cut(s) 56, 667
AclWI GGATC 5 cut(s) 306, 567, 1103, 1116, 1574
AcoI YGGCCR 1 cut(s) 1338
AcuI CTGAAG 1 cut(s) 398
AcyI GRCGYC 3 cut(s) 161, 191, 1044
AdeI CACNNNGTG 1 cut(s) 774
AfaI GTAC 2 cut(s) 284, 818
AfiI CCNNNNNNNGG 7 cut(s) 220, 292, 322, 586, 912, 1280, 1522
AflIII ACRYGT 2 cut(s) 402, 642
AgsI TTSAA 4 cut(s) 24, 442, 451, 1144
AhdI GACNNNNNGTC 1 cut(s) 1253
AjnI CCWGG 3 cut(s) 1028, 1387, 1499
AjuI GAANNNNNNNTTGG 2 cut(s) 60, 92
AluBI AGCT 9 cut(s) 134, 438, 607, 869, 1120, 1129, 1193, 1473, 1528
AluI AGCT 9 cut(s) 134, 438, 607, 869, 1120, 1129, 1193, 1473, 1528
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 4 cut(s) 618, 633, 1313, 1552
AlwI GGATC 5 cut(s) 306, 567, 1103, 1116, 1574
AlwNI CAGNNNCTG 1 cut(s) 322
Ama87I CYCGRG 1 cut(s) 1474
Aor13HI TCCGGA 2 cut(s) 56, 667
AoxI GGCC 4 cut(s) 387, 517, 1338, 1414
ApeKI GCWGC 5 cut(s) 110, 148, 268, 607, 866
Asp718I GGTACC 1 cut(s) 816
AspLEI GCGC 4 cut(s) 268, 500, 1046, 1551
AspS9I GGNCC 1 cut(s) 670
AsuC2I CCSGG 4 cut(s) 81, 821, 836, 1162
AsuHPI GGTGA 7 cut(s) 56, 168, 169, 760, 824, 1031, 1105
AsuII TTCGAA 1 cut(s) 653
AvaI CYCGRG 1 cut(s) 1474
AvaII GGWCC 1 cut(s) 670
AxyI CCTNAGG 1 cut(s) 1553
BamHI GGATCC 1 cut(s) 1108
BanI GGYRCC 4 cut(s) 166, 223, 816, 1043
BanII GRGCYC 1 cut(s) 984
BauI CACGAG 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 315
BbvCI CCTCAGC 2 cut(s) 384, 1469
BbvI GCAGC 5 cut(s) 122, 160, 255, 594, 878
BccI CCATC 7 cut(s) 487, 541, 894, 1034, 1099, 1298, 1392
BceAI ACGGC 1 cut(s) 86
BciT130I CCWGG 3 cut(s) 1030, 1389, 1501
BclI TGATCA 1 cut(s) 684
BcnI CCSGG 4 cut(s) 81, 821, 836, 1162
BcoDI GTCTC 4 cut(s) 618, 633, 1313, 1552
BfaI CTAG 3 cut(s) 368, 960, 1182
BfmI CTRYAG 1 cut(s) 864
BfoI RGCGCY 4 cut(s) 269, 501, 1047, 1552
BglI GCCNNNNNGGC 2 cut(s) 356, 1337
BisI GCNGC 9 cut(s) 111, 149, 189, 269, 390, 608, 708, 753, 867
BlsI GCNGC 9 cut(s) 112, 150, 190, 270, 391, 609, 709, 754, 868
Bme1390I CCNGG 7 cut(s) 81, 821, 836, 1030, 1162, 1389, 1501
Bme18I GGWCC 1 cut(s) 670
BmeRI GACNNNNNGTC 1 cut(s) 1253
BmeT110I CYCGRG 1 cut(s) 1474
BmgT120I GGNCC 1 cut(s) 670
BmiI GGNNCC 9 cut(s) 168, 202, 225, 365, 672, 818, 1045, 1110, 1299
BmrFI CCNGG 7 cut(s) 81, 821, 836, 1030, 1162, 1389, 1501
BmrI ACTGGG 1 cut(s) 766
BmsI GCATC 3 cut(s) 102, 1318, 1408
BmuI ACTGGG 1 cut(s) 766
BpmI CTGGAG 3 cut(s) 299, 323, 473
Bpu10I CCTNAGC 2 cut(s) 384, 1469
Bpu14I TTCGAA 1 cut(s) 653
BpuEI CTTGAG 2 cut(s) 1295, 1424
BpuMI CCSGG 4 cut(s) 81, 821, 836, 1162
BsaHI GRCGYC 3 cut(s) 161, 191, 1044
BsaI GGTCTC 1 cut(s) 1313
BsaJI CCNNGG 4 cut(s) 393, 1081, 1341, 1499
BsaWI WCCGGW 2 cut(s) 56, 667
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bsc4I CCNNNNNNNGG 7 cut(s) 220, 292, 322, 586, 912, 1280, 1522
Bse118I RCCGGY 3 cut(s) 163, 226, 350
Bse1I ACTGG 5 cut(s) 18, 527, 745, 772, 1041
Bse21I CCTNAGG 1 cut(s) 1553
BseAI TCCGGA 2 cut(s) 56, 667
BseBI CCWGG 3 cut(s) 1030, 1389, 1501
BseDI CCNNGG 4 cut(s) 393, 1081, 1341, 1499
BseGI GGATG 5 cut(s) 552, 886, 917, 1045, 1384
BseLI CCNNNNNNNGG 7 cut(s) 220, 292, 322, 586, 912, 1280, 1522
BseMII CTCAG 5 cut(s) 375, 666, 739, 1185, 1483
BseNI ACTGG 5 cut(s) 18, 527, 745, 772, 1041
BseRI GAGGAG 3 cut(s) 386, 602, 636
BseXI GCAGC 5 cut(s) 122, 160, 255, 594, 878
BseYI CCCAGC 2 cut(s) 89, 130
Bsh1236I CGCG 2 cut(s) 404, 644
BshFI GGCC 4 cut(s) 389, 519, 1340, 1416
BshNI GGYRCC 4 cut(s) 166, 223, 816, 1043
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 1474
BslFI GGGAC 1 cut(s) 1558
BslI CCNNNNNNNGG 7 cut(s) 220, 292, 322, 586, 912, 1280, 1522
BsmAI GTCTC 4 cut(s) 618, 633, 1313, 1552
BsmBI CGTCTC 2 cut(s) 633, 1552
BsmFI GGGAC 1 cut(s) 1558
BsmI GAATGC 2 cut(s) 223, 1436
BsnI GGCC 4 cut(s) 389, 519, 1340, 1416
Bso31I GGTCTC 1 cut(s) 1313
BsoBI CYCGRG 1 cut(s) 1474
Bsp119I TTCGAA 1 cut(s) 653
Bsp1286I GDGCHC 2 cut(s) 315, 984
Bsp13I TCCGGA 2 cut(s) 56, 667
Bsp143I GATC 5 cut(s) 298, 572, 684, 1108, 1566
Bsp19I CCATGG 1 cut(s) 393
BspANI GGCC 4 cut(s) 389, 519, 1340, 1416
BspCNI CTCAG 5 cut(s) 376, 667, 738, 1186, 1482
BspEI TCCGGA 2 cut(s) 56, 667
BspFNI CGCG 2 cut(s) 404, 644
BspHI TCATGA 1 cut(s) 858
BspLI GGNNCC 9 cut(s) 168, 202, 225, 365, 672, 818, 1045, 1110, 1299
BspMAI CTGCAG 1 cut(s) 868
BspPI GGATC 5 cut(s) 306, 567, 1103, 1116, 1574
BspT104I TTCGAA 1 cut(s) 653
BspT107I GGYRCC 4 cut(s) 166, 223, 816, 1043
BspTNI GGTCTC 1 cut(s) 1313
BsrFI RCCGGY 3 cut(s) 163, 226, 350
BsrI ACTGG 5 cut(s) 18, 527, 745, 772, 1041
BssAI RCCGGY 3 cut(s) 163, 226, 350
BssECI CCNNGG 4 cut(s) 393, 1081, 1341, 1499
BssMI GATC 5 cut(s) 298, 572, 684, 1108, 1566
BssNI GRCGYC 3 cut(s) 161, 191, 1044
BssSI CACGAG 1 cut(s) 247
BssT1I CCWWGG 2 cut(s) 393, 1081
Bst2BI CACGAG 1 cut(s) 247
Bst2UI CCWGG 3 cut(s) 1030, 1389, 1501
Bst4CI ACNGT 6 cut(s) 157, 180, 599, 698, 880, 1453
Bst6I CTCTTC 1 cut(s) 81
BstACI GRCGYC 3 cut(s) 161, 191, 1044
BstBI TTCGAA 1 cut(s) 653
BstC8I GCNNGC 5 cut(s) 171, 228, 352, 932, 1530
BstDEI CTNAG 7 cut(s) 384, 675, 725, 1061, 1194, 1469, 1553
BstDSI CCRYGG 1 cut(s) 393
BstEII GGTNACC 1 cut(s) 766
BstF5I GGATG 5 cut(s) 552, 886, 917, 1045, 1384
BstFNI CGCG 2 cut(s) 404, 644
BstH2I RGCGCY 4 cut(s) 269, 501, 1047, 1552
BstHHI GCGC 4 cut(s) 268, 500, 1046, 1551
BstKTI GATC 5 cut(s) 301, 575, 687, 1111, 1569
BstMAI GTCTC 4 cut(s) 618, 633, 1313, 1552
BstMBI GATC 5 cut(s) 298, 572, 684, 1108, 1566
BstNI CCWGG 3 cut(s) 1030, 1389, 1501
BstPI GGTNACC 1 cut(s) 766
BstSCI CCNGG 7 cut(s) 79, 819, 834, 1028, 1160, 1387, 1499
BstSFI CTRYAG 1 cut(s) 864
BstUI CGCG 2 cut(s) 404, 644
BstV1I GCAGC 5 cut(s) 122, 160, 255, 594, 878
BstX2I RGATCY 3 cut(s) 298, 572, 1108
BstXI CCANNNNNNTGG 1 cut(s) 1036
BstYI RGATCY 3 cut(s) 298, 572, 1108
Bsu36I CCTNAGG 1 cut(s) 1553
BsuRI GGCC 4 cut(s) 389, 519, 1340, 1416
BtgI CCRYGG 1 cut(s) 393
BtsCI GGATG 5 cut(s) 552, 886, 917, 1045, 1384
BtsIMutI CAGTG 1 cut(s) 779
Cac8I GCNNGC 5 cut(s) 171, 228, 352, 932, 1530
CaiI CAGNNNCTG 1 cut(s) 322
CciI TCATGA 1 cut(s) 858
CfoI GCGC 4 cut(s) 268, 500, 1046, 1551
Cfr10I RCCGGY 3 cut(s) 163, 226, 350
Cfr13I GGNCC 1 cut(s) 670
CseI GACGC 6 cut(s) 162, 169, 180, 328, 365, 650
Csp6I GTAC 2 cut(s) 283, 817
CviAII CATG 7 cut(s) 394, 859, 923, 1201, 1260, 1280, 1302
CviQI GTAC 2 cut(s) 283, 817
DdeI CTNAG 7 cut(s) 384, 675, 725, 1061, 1194, 1469, 1553
DinI GGCGCC 1 cut(s) 1045
DpnI GATC 5 cut(s) 300, 574, 686, 1110, 1568
DpnII GATC 5 cut(s) 298, 572, 684, 1108, 1566
DraIII CACNNNGTG 1 cut(s) 774
DriI GACNNNNNGTC 1 cut(s) 1253
EaeI YGGCCR 1 cut(s) 1338
Eam1104I CTCTTC 1 cut(s) 81
Eam1105I GACNNNNNGTC 1 cut(s) 1253
EarI CTCTTC 1 cut(s) 81
Eco130I CCWWGG 2 cut(s) 393, 1081
Eco24I GRGCYC 1 cut(s) 984
Eco31I GGTCTC 1 cut(s) 1313
Eco32I GATATC 1 cut(s) 1481
Eco47I GGWCC 1 cut(s) 670
Eco57I CTGAAG 1 cut(s) 398
Eco81I CCTNAGG 1 cut(s) 1553
Eco88I CYCGRG 1 cut(s) 1474
Eco91I GGTNACC 1 cut(s) 766
EcoO65I GGTNACC 1 cut(s) 766
EcoRII CCWGG 3 cut(s) 1028, 1387, 1499
EcoRV GATATC 1 cut(s) 1481
EcoT14I CCWWGG 2 cut(s) 393, 1081
EcoT22I ATGCAT 2 cut(s) 1281, 1436
EcoT38I GRGCYC 1 cut(s) 984
EgeI GGCGCC 1 cut(s) 1045
EheI GGCGCC 1 cut(s) 1045
ErhI CCWWGG 2 cut(s) 393, 1081
Esp3I CGTCTC 2 cut(s) 633, 1552
FaeI CATG 7 cut(s) 397, 862, 926, 1204, 1263, 1283, 1305
FaqI GGGAC 1 cut(s) 1558
FatI CATG 7 cut(s) 393, 858, 922, 1200, 1259, 1279, 1301
FauI CCCGC 4 cut(s) 127, 342, 413, 544
FauNDI CATATG 1 cut(s) 1005
FbaI TGATCA 1 cut(s) 684
FblI GTMKAC 1 cut(s) 140
Fnu4HI GCNGC 9 cut(s) 111, 149, 189, 269, 390, 608, 708, 753, 867
FokI GGATG 5 cut(s) 559, 873, 904, 1052, 1371
FriOI GRGCYC 1 cut(s) 984
Fsp4HI GCNGC 9 cut(s) 111, 149, 189, 269, 390, 608, 708, 753, 867
FspBI CTAG 3 cut(s) 368, 960, 1182
GlaI GCGC 4 cut(s) 267, 499, 1045, 1550
GluI GCNGC 9 cut(s) 111, 149, 189, 269, 390, 608, 708, 753, 867
GsaI CCCAGC 2 cut(s) 93, 134
GsuI CTGGAG 3 cut(s) 299, 323, 473
HaeII RGCGCY 4 cut(s) 269, 501, 1047, 1552
HaeIII GGCC 4 cut(s) 389, 519, 1340, 1416
HgaI GACGC 6 cut(s) 162, 169, 180, 328, 365, 650
HhaI GCGC 4 cut(s) 268, 500, 1046, 1551
Hin1I GRCGYC 3 cut(s) 161, 191, 1044
Hin1II CATG 7 cut(s) 397, 862, 926, 1204, 1263, 1283, 1305
Hin6I GCGC 4 cut(s) 266, 498, 1044, 1549
HinP1I GCGC 4 cut(s) 266, 498, 1044, 1549
HincII GTYRAC 3 cut(s) 141, 183, 883
HindII GTYRAC 3 cut(s) 141, 183, 883
HindIII AAGCTT 1 cut(s) 1127
HphI GGTGA 7 cut(s) 56, 168, 169, 760, 824, 1031, 1105
Hpy166II GTNNAC 7 cut(s) 106, 141, 183, 244, 634, 883, 1366
Hpy188I TCNGA 5 cut(s) 363, 515, 577, 689, 1349
Hpy8I GTNNAC 7 cut(s) 106, 141, 183, 244, 634, 883, 1366
Hpy99I CGWCG 2 cut(s) 101, 145
HpyAV CCTTC 7 cut(s) 81, 256, 340, 537, 586, 1370, 1557
HpyCH4III ACNGT 6 cut(s) 157, 180, 599, 698, 880, 1453
HpyCH4V TGCA 6 cut(s) 271, 760, 866, 1279, 1399, 1434
HpyF3I CTNAG 7 cut(s) 384, 675, 725, 1061, 1194, 1469, 1553
Hsp92I GRCGYC 3 cut(s) 161, 191, 1044
Hsp92II CATG 7 cut(s) 397, 862, 926, 1204, 1263, 1283, 1305
HspAI GCGC 4 cut(s) 266, 498, 1044, 1549
KasI GGCGCC 1 cut(s) 1043
Kpn2I TCCGGA 2 cut(s) 56, 667
KpnI GGTACC 1 cut(s) 820
KroI GCCGGC 2 cut(s) 226, 350
KroNI GCCGGC 2 cut(s) 228, 352
Ksp22I TGATCA 1 cut(s) 684
Kzo9I GATC 5 cut(s) 298, 572, 684, 1108, 1566
LmnI GCTCC 9 cut(s) 206, 237, 318, 363, 373, 492, 1190, 1303, 1525
Lsp1109I GCAGC 5 cut(s) 122, 160, 255, 594, 878
LweI GCATC 3 cut(s) 102, 1318, 1408
MaeI CTAG 3 cut(s) 368, 960, 1182
MalI GATC 5 cut(s) 300, 574, 686, 1110, 1568
MboI GATC 5 cut(s) 298, 572, 684, 1108, 1566
MboII GAAGA 5 cut(s) 33, 68, 401, 404, 713
MfeI CAATTG 1 cut(s) 272
MflI RGATCY 3 cut(s) 298, 572, 1108
MhlI GDGCHC 2 cut(s) 315, 984
MluCI AATT 2 cut(s) 272, 531
MluI ACGCGT 2 cut(s) 402, 642
Mly113I GGCGCC 1 cut(s) 1044
MlyI GAGTC 7 cut(s) 239, 314, 320, 658, 706, 867, 962
MmeI TCCRAC 2 cut(s) 600, 945
Mph1103I ATGCAT 2 cut(s) 1281, 1436
MreI CGCCGGCG 1 cut(s) 350
MroI TCCGGA 2 cut(s) 56, 667
MroNI GCCGGC 2 cut(s) 226, 350
MseI TTAA 2 cut(s) 534, 1125
MslI CAYNNNNRTG 2 cut(s) 1184, 1205
MspA1I CMGCKG 2 cut(s) 134, 869
MspR9I CCNGG 7 cut(s) 81, 821, 836, 1030, 1162, 1389, 1501
MunI CAATTG 1 cut(s) 272
Mva1269I GAATGC 2 cut(s) 223, 1436
MvaI CCWGG 3 cut(s) 1030, 1389, 1501
MvnI CGCG 2 cut(s) 404, 644
NaeI GCCGGC 2 cut(s) 228, 352
NarI GGCGCC 1 cut(s) 1044
NciI CCSGG 4 cut(s) 81, 821, 836, 1162
NcoI CCATGG 1 cut(s) 393
NdeI CATATG 1 cut(s) 1005
NdeII GATC 5 cut(s) 298, 572, 684, 1108, 1566
NgoMIV GCCGGC 2 cut(s) 226, 350
NlaIII CATG 7 cut(s) 397, 862, 926, 1204, 1263, 1283, 1305
NlaIV GGNNCC 9 cut(s) 168, 202, 225, 365, 672, 818, 1045, 1110, 1299
NmeAIII GCCGAG 4 cut(s) 594, 636, 1366, 1570
NmuCI GTSAC 9 cut(s) 157, 174, 467, 766, 1255, 1423, 1443, 1453, 1493
NsiI ATGCAT 2 cut(s) 1281, 1436
NspV TTCGAA 1 cut(s) 653
PaeR7I CTCGAG 1 cut(s) 1474
PagI TCATGA 1 cut(s) 858
PcsI WCGNNNNNNNCGW 1 cut(s) 1365
PctI GAATGC 2 cut(s) 223, 1436
PdiI GCCGGC 2 cut(s) 228, 352
PfeI GAWTC 5 cut(s) 481, 650, 855, 1086, 1144
PflFI GACNNNGTC 1 cut(s) 599
PflMI CCANNNNNTGG 2 cut(s) 322, 1522
PfoI TCCNGGA 1 cut(s) 1387
PkrI GCNGC 9 cut(s) 112, 150, 190, 270, 391, 609, 709, 754, 868
PleI GAGTC 7 cut(s) 239, 313, 320, 658, 706, 867, 962
PluTI GGCGCC 1 cut(s) 1047
PpsI GAGTC 7 cut(s) 239, 313, 320, 658, 706, 867, 962
PsiI TTATAA 1 cut(s) 1226
Psp6I CCWGG 3 cut(s) 1028, 1387, 1499
PspEI GGTNACC 1 cut(s) 766
PspFI CCCAGC 2 cut(s) 89, 130
PspGI CCWGG 3 cut(s) 1028, 1387, 1499
PspN4I GGNNCC 9 cut(s) 168, 202, 225, 365, 672, 818, 1045, 1110, 1299
PspPI GGNCC 1 cut(s) 670
PstI CTGCAG 1 cut(s) 868
PstNI CAGNNNCTG 1 cut(s) 322
PsuI RGATCY 3 cut(s) 298, 572, 1108
PsyI GACNNNGTC 1 cut(s) 599
PvuII CAGCTG 2 cut(s) 134, 869
RsaI GTAC 2 cut(s) 284, 818
RsaNI GTAC 2 cut(s) 283, 817
RseI CAYNNNNRTG 2 cut(s) 1184, 1205
SalI GTCGAC 1 cut(s) 139
SaqAI TTAA 2 cut(s) 534, 1125
SatI GCNGC 9 cut(s) 111, 149, 189, 269, 390, 608, 708, 753, 867
Sau3AI GATC 5 cut(s) 298, 572, 684, 1108, 1566
Sau96I GGNCC 1 cut(s) 670
SchI GAGTC 7 cut(s) 239, 314, 320, 658, 706, 867, 962
ScrFI CCNGG 7 cut(s) 81, 821, 836, 1030, 1162, 1389, 1501
SduI GDGCHC 2 cut(s) 315, 984
SfaNI GCATC 3 cut(s) 102, 1318, 1408
SfcI CTRYAG 1 cut(s) 864
SfoI GGCGCC 1 cut(s) 1045
Sfr274I CTCGAG 1 cut(s) 1474
SfuI TTCGAA 1 cut(s) 653
SgrAI CRCCGGYG 2 cut(s) 163, 350
SinI GGWCC 1 cut(s) 670
SlaI CTCGAG 1 cut(s) 1474
SmiMI CAYNNNNRTG 2 cut(s) 1184, 1205
SmlI CTYRAG 3 cut(s) 1310, 1403, 1474
SmoI CTYRAG 3 cut(s) 1310, 1403, 1474
Sse9I AATT 2 cut(s) 272, 531
SspDI GGCGCC 1 cut(s) 1043
SspI AATATT 1 cut(s) 463
SspMI CTAG 3 cut(s) 368, 960, 1182
StyD4I CCNGG 7 cut(s) 79, 819, 834, 1028, 1160, 1387, 1499
StyI CCWWGG 2 cut(s) 393, 1081
TaaI ACNGT 6 cut(s) 157, 180, 599, 698, 880, 1453
TaqI TCGA 4 cut(s) 140, 653, 1359, 1475
TaqII GACCGA 1 cut(s) 1442
TasI AATT 2 cut(s) 272, 531
TauI GCSGC 4 cut(s) 191, 392, 710, 755
TfiI GAWTC 5 cut(s) 481, 650, 855, 1086, 1144
Tru1I TTAA 2 cut(s) 534, 1125
Tru9I TTAA 2 cut(s) 534, 1125
TscAI CASTG 1 cut(s) 779
TseFI GTSAC 9 cut(s) 157, 174, 467, 766, 1255, 1423, 1443, 1453, 1493
TseI GCWGC 5 cut(s) 110, 148, 268, 607, 866
Tsp45I GTSAC 9 cut(s) 157, 174, 467, 766, 1255, 1423, 1443, 1453, 1493
TspDTI ATGAA 2 cut(s) 33, 847
TspGWI ACGGA 3 cut(s) 651, 1226, 1462
TspRI CASTG 1 cut(s) 779
Tth111I GACNNNGTC 1 cut(s) 599
Van91I CCANNNNNTGG 2 cut(s) 322, 1522
VpaK11BI GGWCC 1 cut(s) 670
XhoI CTCGAG 1 cut(s) 1474
XmiI GTMKAC 1 cut(s) 140
XspI CTAG 3 cut(s) 368, 960, 1182
Zsp2I ATGCAT 2 cut(s) 1281, 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.