Rroxscaffold_3G00222150

Phospho-2-dehydro-3-deoxyheptonate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0015832
Physical Location & Seq
Forward (+)
5148118 .. 5152376
4259 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_3G00222150.1

Sequence Viewer

Length: 1620 bp
ATGGCTCTCACGAGCAGCAGCTCAATCATTACCCCCAAATCTTCCTTCATTCAAACCCAGTCGACTCTCTTACCCTCCAAACCTCACCAACCTTCTCTCCCCGTCAGGCCCATCAGGCCCATCGTCGCCGTCCACGCAGCCGAGCCCGCCAAGAATCCAGCTGTGTCCACCAAGCCGCAGACGGTGCCAGCTTCGCCGTCAACTGCGGCGTCTAATGGGTCGAAAAGCTGGAATGCGGTGCCTGCGAAATGGAGTTTGGACACGTGGAAATCCAAGAAGGCGGTGCAATTGCCGGAGTACCCGAATAAAGAGGATCTGGAGTCGGTGCTCCGGACGATTGAGTCTTTCCCGCCGATCGTCTTCGCCGGCGAGGCTCGGAGTTTAGAGGAGCGTCTCGCTGAGGCTTCCATGGGAAACGCGTTTCTTCTTCAAGGTGGGGATTGTGCGGAGAGCTTCAAGGAGTTCAATGCCAATAATATTCGTGACACCTTCAGAATTCTGCTCCAGATGGGCGTCGTTTTGATGTTCGGAGGCCAAATGCCAGTAATTAAGGTGGGAAGGATGGCGGGTCAGTTTGCGAAGCCGAGATCCGACCCCTTTGAGGAGAAGGATGGTGTGAAACTGCCGAGTTACAGAGGGGACAATGTGAACGGAGACACGTTTGATTCAAAGTCAAGGACTCCGGACCCTGAGAGATTGATCAGAGCCTACTGTCAATCTGCGGCGACTCTCAACCTTCTCAGGGCTTTCGCAACCGGTGGTTATGCGGCTATGCAGAGAGTTACCCAGTGGAACTTGGATTTCACAGAGCACAGCGAACAGGGTGATAGGTACCGAGAACTAGCTCACCGGGTTGATGAGGCCCTTGGATTCATGACTGCAGCTGGGCTCACAGTTGACCATCCTATCATGACAACCACAGAGTTCTGGACATCCCATGAGTGCTTGCTTTTGCCTTATGAACAGTCGCTCACTAGGTTGGACTCAACTTCTGGGCTCTACTATGATTGCTCTGCCCATATGCTTTGGGTCGGTGAGCGAACCAGGCAACTGGATGGTGCCCATATTGAGTTCCTAAGAGGTGTTGCTAATCCCCTGGGAATCAAGGTGAGTGATAAGATGGATCCCAATGAGCTTGTTAAGCTCATTGAGATTTTGAATCCTCAAAATAAAGCCGGGAGGATAACGGTGATCACAAGAATGGGAGCTGAGAACATGAGAGTGAAACTTCCTCATCTTATTAGGGCAGTCCGTAGGGCAGGACAAATTGTCACATGGGTCAGCGACCCTATGCATGGAAACACCATCAAGGCTCCATGTGGTCTCAAGACACGCCCTTTTGATGCCATAAGGGCTGAGGTCAGAGCATTCTTTGATGTGCACGACCAAGAAGGAAGCCACCCAGGAGGTGTTCATCTGGAGATGACGGGCCAAAATGTGACTGAATGCATTGGAGGATCACGGACTGTGACATTCGACGACCTTAGCTCGCGCTACCATACTCACTGTGACCCGAGGCTAAATGCCTCCCAATCTCTTGAGCTTGCCTTCATTATTGCTGAGCGCCTGAGGAGACGAAGGATCAAGTCACAACTCTCTGTTGCCTCGTTAGGTCTGTAG

Protein Analysis

539

Amino Acids

59.66

Weight (kDa)

8.72

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAHP_synth_2 PF01474 84 - 520 3.3e-204 Class-II DAHP synthetase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22410
fragaria_vesca FvH4_5g36810
malus_domestica MD08G1186700.v1.1 MD15G1372600.v1.1
prunus_persica Prupe.1G521200_v2.0.a1
pyrus_communis pycom08g16050 pycom15g33360
rosa_chinensis RchiOBHm_Chr7g0243371
rosa_laevigata RLG00000000852
rosa_multiflora Rmu_sc0001036.1_g000028 Rmu_sc0001461.1_g000106
rosa_roxburghii Rroxscaffold_3G00222150
rosa_rugosa Rorug07G0318600
rosa_samantha Rh7BG444800
rosa_wichuraiana Rw7G039490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 340
Acc65I GGTACC 1 cut(s) 831
AccB1I GGYRCC 4 cut(s) 184, 238, 831, 1058
AccI GTMKAC 1 cut(s) 62
AccII CGCG 2 cut(s) 419, 1492
AccIII TCCGGA 2 cut(s) 330, 682
AclWI GGATC 6 cut(s) 321, 582, 1118, 1131, 1465, 1589
AcsI RAATTY 1 cut(s) 495
AcuI CTGAAG 1 cut(s) 475
AcvI CACGTG 1 cut(s) 264
AcyI GRCGYC 2 cut(s) 209, 513
AfaI GTAC 2 cut(s) 299, 833
AfiI CCNNNNNNNGG 3 cut(s) 601, 742, 1295
AflIII ACRYGT 3 cut(s) 261, 417, 657
AgeI ACCGGT 1 cut(s) 755
AgsI TTSAA 6 cut(s) 53, 431, 457, 466, 669, 1159
AhdI GACNNNNNGTC 1 cut(s) 1268
AjnI CCWGG 3 cut(s) 1043, 1095, 1402
AjuI GAANNNNNNNTTGG 4 cut(s) 29, 61, 239, 271
Alw21I GWGCWC 3 cut(s) 330, 813, 1383
Alw26I GTCTC 4 cut(s) 398, 648, 1328, 1567
Alw44I GTGCAC 1 cut(s) 1379
AlwI GGATC 6 cut(s) 321, 582, 1118, 1131, 1465, 1589
Ama87I CYCGRG 1 cut(s) 1513
Aor13HI TCCGGA 2 cut(s) 330, 682
AoxI GGCC 5 cut(s) 107, 116, 532, 861, 1429
ApaLI GTGCAC 1 cut(s) 1379
ApeKI GCWGC 4 cut(s) 15, 18, 137, 881
ApoI RAATTY 1 cut(s) 495
AsiGI ACCGGT 1 cut(s) 755
Asp718I GGTACC 1 cut(s) 831
AspLEI GCGC 2 cut(s) 1494, 1566
AspS9I GGNCC 5 cut(s) 108, 117, 685, 862, 1429
AsuC2I CCSGG 2 cut(s) 851, 1177
AsuHPI GGTGA 6 cut(s) 77, 836, 839, 1046, 1120, 1201
AvaI CYCGRG 1 cut(s) 1513
AvaII GGWCC 1 cut(s) 685
AxyI CCTNAGG 1 cut(s) 1568
BaeGI GKGCMC 2 cut(s) 1063, 1383
BamHI GGATCC 1 cut(s) 1123
BanI GGYRCC 4 cut(s) 184, 238, 831, 1058
BanII GRGCYC 3 cut(s) 147, 891, 999
BauI CACGAG 1 cut(s) 10
BbrPI CACGTG 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 352
Bbv12I GWGCWC 3 cut(s) 330, 813, 1383
BbvCI CCTCAGC 2 cut(s) 399, 1356
BbvI GCAGC 4 cut(s) 27, 30, 149, 893
BccI CCATC 9 cut(s) 119, 128, 502, 556, 605, 909, 1049, 1114, 1313
BceAI ACGGC 2 cut(s) 113, 181
BciT130I CCWGG 3 cut(s) 1045, 1097, 1404
BclI TGATCA 2 cut(s) 699, 1191
BcnI CCSGG 2 cut(s) 851, 1177
BcoDI GTCTC 4 cut(s) 398, 648, 1328, 1567
BfaI CTAG 2 cut(s) 842, 975
BfmI CTRYAG 2 cut(s) 879, 1616
BfoI RGCGCY 1 cut(s) 1567
BglI GCCNNNNNGGC 3 cut(s) 115, 371, 1352
BisI GCNGC 8 cut(s) 16, 19, 138, 176, 207, 723, 768, 882
BlpI GCTNAGC 1 cut(s) 1560
BlsI GCNGC 8 cut(s) 17, 20, 139, 177, 208, 724, 769, 883
Bme1390I CCNGG 5 cut(s) 851, 1045, 1097, 1177, 1404
Bme18I GGWCC 1 cut(s) 685
BmeRI GACNNNNNGTC 1 cut(s) 1268
BmeT110I CYCGRG 1 cut(s) 1513
BmgT120I GGNCC 5 cut(s) 108, 117, 685, 862, 1429
BmiI GGNNCC 7 cut(s) 186, 240, 687, 833, 1060, 1125, 1314
BmrFI CCNGG 5 cut(s) 851, 1045, 1097, 1177, 1404
BmrI ACTGGG 2 cut(s) 52, 781
BmsI GCATC 1 cut(s) 1333
BmuI ACTGGG 2 cut(s) 52, 781
BpiI GAAGAC 1 cut(s) 352
BpmI CTGGAG 3 cut(s) 338, 488, 1439
Bpu10I CCTNAGC 3 cut(s) 399, 1356, 1484
Bpu1102I GCTNAGC 1 cut(s) 1560
BpuEI CTTGAG 2 cut(s) 1310, 1559
BpuMI CCSGG 2 cut(s) 851, 1177
BsaAI YACGTR 1 cut(s) 264
BsaHI GRCGYC 2 cut(s) 209, 513
BsaI GGTCTC 1 cut(s) 1328
BsaJI CCNNGG 6 cut(s) 408, 865, 1095, 1096, 1402, 1514
BsaWI WCCGGW 3 cut(s) 330, 682, 755
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 3 cut(s) 601, 742, 1295
Bse118I RCCGGY 2 cut(s) 365, 755
Bse1I ACTGG 4 cut(s) 58, 542, 787, 1056
Bse21I CCTNAGG 1 cut(s) 1568
BseAI TCCGGA 2 cut(s) 330, 682
BseBI CCWGG 3 cut(s) 1045, 1097, 1404
BseDI CCNNGG 6 cut(s) 408, 865, 1095, 1096, 1402, 1514
BseGI GGATG 5 cut(s) 567, 616, 901, 932, 1060
BseLI CCNNNNNNNGG 3 cut(s) 601, 742, 1295
BseMII CTCAG 7 cut(s) 390, 681, 754, 1200, 1347, 1551, 1559
BseNI ACTGG 4 cut(s) 58, 542, 787, 1056
BseRI GAGGAG 3 cut(s) 401, 617, 1585
BseSI GKGCMC 2 cut(s) 1063, 1383
BseXI GCAGC 4 cut(s) 27, 30, 149, 893
BseYI CCCAGC 1 cut(s) 884
Bsh1236I CGCG 2 cut(s) 419, 1492
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 5 cut(s) 109, 118, 534, 863, 1431
BshNI GGYRCC 4 cut(s) 184, 238, 831, 1058
BshTI ACCGGT 1 cut(s) 755
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 3 cut(s) 330, 813, 1383
BsiHKCI CYCGRG 1 cut(s) 1513
BsiSI CCGG 7 cut(s) 293, 331, 366, 683, 756, 850, 1176
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 3 cut(s) 601, 742, 1295
BsmAI GTCTC 4 cut(s) 398, 648, 1328, 1567
BsmBI CGTCTC 2 cut(s) 398, 1567
BsmFI GGGAC 1 cut(s) 653
BsmI GAATGC 3 cut(s) 238, 1367, 1451
BsnI GGCC 5 cut(s) 109, 118, 534, 863, 1431
Bso31I GGTCTC 1 cut(s) 1328
BsoBI CYCGRG 1 cut(s) 1513
Bsp1286I GDGCHC 7 cut(s) 147, 330, 813, 891, 999, 1063, 1383
Bsp13I TCCGGA 2 cut(s) 330, 682
Bsp143I GATC 8 cut(s) 313, 354, 587, 699, 1123, 1191, 1457, 1581
Bsp1720I GCTNAGC 1 cut(s) 1560
Bsp19I CCATGG 1 cut(s) 408
BspANI GGCC 5 cut(s) 109, 118, 534, 863, 1431
BspCNI CTCAG 7 cut(s) 391, 682, 753, 1201, 1348, 1552, 1560
BspEI TCCGGA 2 cut(s) 330, 682
BspFNI CGCG 2 cut(s) 419, 1492
BspHI TCATGA 2 cut(s) 873, 909
BspLI GGNNCC 7 cut(s) 186, 240, 687, 833, 1060, 1125, 1314
BspMAI CTGCAG 1 cut(s) 883
BspPI GGATC 6 cut(s) 321, 582, 1118, 1131, 1465, 1589
BspT107I GGYRCC 4 cut(s) 184, 238, 831, 1058
BspTNI GGTCTC 1 cut(s) 1328
BsrFI RCCGGY 2 cut(s) 365, 755
BsrI ACTGG 4 cut(s) 58, 542, 787, 1056
BssAI RCCGGY 2 cut(s) 365, 755
BssECI CCNNGG 6 cut(s) 408, 865, 1095, 1096, 1402, 1514
BssMI GATC 8 cut(s) 313, 354, 587, 699, 1123, 1191, 1457, 1581
BssNI GRCGYC 2 cut(s) 209, 513
BssSI CACGAG 1 cut(s) 10
BssT1I CCWWGG 2 cut(s) 408, 865
Bst2BI CACGAG 1 cut(s) 10
Bst2UI CCWGG 3 cut(s) 1045, 1097, 1404
Bst4CI ACNGT 7 cut(s) 184, 713, 895, 966, 1189, 1468, 1508
BstACI GRCGYC 2 cut(s) 209, 513
BstAPI GCANNNNNTGC 1 cut(s) 184
BstBAI YACGTR 1 cut(s) 264
BstC8I GCNNGC 7 cut(s) 147, 189, 243, 367, 947, 1490, 1545
BstDEI CTNAG 9 cut(s) 399, 690, 740, 1076, 1209, 1356, 1484, 1560, 1568
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 5 cut(s) 567, 616, 901, 932, 1060
BstFNI CGCG 2 cut(s) 419, 1492
BstH2I RGCGCY 1 cut(s) 1567
BstHHI GCGC 2 cut(s) 1494, 1566
BstKTI GATC 8 cut(s) 316, 357, 590, 702, 1126, 1194, 1460, 1584
BstMAI GTCTC 4 cut(s) 398, 648, 1328, 1567
BstMBI GATC 8 cut(s) 313, 354, 587, 699, 1123, 1191, 1457, 1581
BstMCI CGRYCG 1 cut(s) 357
BstNI CCWGG 3 cut(s) 1045, 1097, 1404
BstSCI CCNGG 5 cut(s) 849, 1043, 1095, 1175, 1402
BstSFI CTRYAG 2 cut(s) 879, 1616
BstSLI GKGCMC 2 cut(s) 1063, 1383
BstUI CGCG 2 cut(s) 419, 1492
BstV1I GCAGC 4 cut(s) 27, 30, 149, 893
BstV2I GAAGAC 1 cut(s) 352
BstX2I RGATCY 3 cut(s) 313, 587, 1123
BstXI CCANNNNNNTGG 1 cut(s) 1051
BstYI RGATCY 3 cut(s) 313, 587, 1123
Bsu36I CCTNAGG 1 cut(s) 1568
BsuRI GGCC 5 cut(s) 109, 118, 534, 863, 1431
BtgI CCRYGG 1 cut(s) 408
BtsCI GGATG 5 cut(s) 567, 616, 901, 932, 1060
BtsIMutI CAGTG 2 cut(s) 794, 1504
Cac8I GCNNGC 7 cut(s) 147, 189, 243, 367, 947, 1490, 1545
CciI TCATGA 2 cut(s) 873, 909
CfoI GCGC 2 cut(s) 1494, 1566
Cfr10I RCCGGY 2 cut(s) 365, 755
Cfr13I GGNCC 5 cut(s) 108, 117, 685, 862, 1429
CseI GACGC 3 cut(s) 198, 380, 502
Csp6I GTAC 2 cut(s) 298, 832
CspAI ACCGGT 1 cut(s) 755
CviAII CATG 8 cut(s) 409, 874, 910, 938, 1216, 1275, 1295, 1317
CviQI GTAC 2 cut(s) 298, 832
DdeI CTNAG 9 cut(s) 399, 690, 740, 1076, 1209, 1356, 1484, 1560, 1568
DpnI GATC 8 cut(s) 315, 356, 589, 701, 1125, 1193, 1459, 1583
DpnII GATC 8 cut(s) 313, 354, 587, 699, 1123, 1191, 1457, 1581
DrdI GACNNNNNNGTC 1 cut(s) 340
DriI GACNNNNNGTC 1 cut(s) 1268
DseDI GACNNNNNNGTC 1 cut(s) 340
Eam1105I GACNNNNNGTC 1 cut(s) 1268
Eco130I CCWWGG 2 cut(s) 408, 865
Eco24I GRGCYC 3 cut(s) 147, 891, 999
Eco31I GGTCTC 1 cut(s) 1328
Eco47I GGWCC 1 cut(s) 685
Eco57I CTGAAG 1 cut(s) 475
Eco72I CACGTG 1 cut(s) 264
Eco81I CCTNAGG 1 cut(s) 1568
Eco88I CYCGRG 1 cut(s) 1513
EcoO109I RGGNCCY 1 cut(s) 862
EcoRI GAATTC 1 cut(s) 495
EcoRII CCWGG 3 cut(s) 1043, 1095, 1402
EcoT14I CCWWGG 2 cut(s) 408, 865
EcoT22I ATGCAT 2 cut(s) 1296, 1451
EcoT38I GRGCYC 3 cut(s) 147, 891, 999
ErhI CCWWGG 2 cut(s) 408, 865
Esp3I CGTCTC 2 cut(s) 398, 1567
FaeI CATG 8 cut(s) 412, 877, 913, 941, 1219, 1278, 1298, 1320
FaqI GGGAC 1 cut(s) 653
FatI CATG 8 cut(s) 408, 873, 909, 937, 1215, 1274, 1294, 1316
FauI CCCGC 3 cut(s) 154, 357, 559
FauNDI CATATG 1 cut(s) 1020
FbaI TGATCA 2 cut(s) 699, 1191
FblI GTMKAC 1 cut(s) 62
Fnu4HI GCNGC 8 cut(s) 16, 19, 138, 176, 207, 723, 768, 882
FokI GGATG 5 cut(s) 574, 623, 888, 919, 1067
FriOI GRGCYC 3 cut(s) 147, 891, 999
Fsp4HI GCNGC 8 cut(s) 16, 19, 138, 176, 207, 723, 768, 882
FspBI CTAG 2 cut(s) 842, 975
GlaI GCGC 2 cut(s) 1493, 1565
GluI GCNGC 8 cut(s) 16, 19, 138, 176, 207, 723, 768, 882
GsaI CCCAGC 1 cut(s) 888
GsuI CTGGAG 3 cut(s) 338, 488, 1439
HaeII RGCGCY 1 cut(s) 1567
HaeIII GGCC 5 cut(s) 109, 118, 534, 863, 1431
HapII CCGG 7 cut(s) 293, 331, 366, 683, 756, 850, 1176
HgaI GACGC 3 cut(s) 198, 380, 502
HhaI GCGC 2 cut(s) 1494, 1566
Hin1I GRCGYC 2 cut(s) 209, 513
Hin1II CATG 8 cut(s) 412, 877, 913, 941, 1219, 1278, 1298, 1320
Hin6I GCGC 2 cut(s) 1492, 1564
HinP1I GCGC 2 cut(s) 1492, 1564
HincII GTYRAC 3 cut(s) 63, 201, 898
HindII GTYRAC 3 cut(s) 63, 201, 898
HpaII CCGG 7 cut(s) 293, 331, 366, 683, 756, 850, 1176
HphI GGTGA 6 cut(s) 77, 836, 839, 1046, 1120, 1201
Hpy166II GTNNAC 7 cut(s) 63, 133, 168, 201, 649, 898, 1381
Hpy188I TCNGA 6 cut(s) 378, 494, 530, 592, 704, 1364
Hpy8I GTNNAC 7 cut(s) 63, 133, 168, 201, 649, 898, 1381
Hpy99I CGWCG 3 cut(s) 128, 518, 1481
HpyCH4III ACNGT 7 cut(s) 184, 713, 895, 966, 1189, 1468, 1508
HpyCH4IV ACGT 2 cut(s) 263, 659
HpyCH4V TGCA 6 cut(s) 286, 775, 881, 1294, 1381, 1449
HpyF3I CTNAG 9 cut(s) 399, 690, 740, 1076, 1209, 1356, 1484, 1560, 1568
HpySE526I ACGT 2 cut(s) 263, 659
Hsp92I GRCGYC 2 cut(s) 209, 513
Hsp92II CATG 8 cut(s) 412, 877, 913, 941, 1219, 1278, 1298, 1320
HspAI GCGC 2 cut(s) 1492, 1564
Kpn2I TCCGGA 2 cut(s) 330, 682
KpnI GGTACC 1 cut(s) 835
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Ksp22I TGATCA 2 cut(s) 699, 1191
Kzo9I GATC 8 cut(s) 313, 354, 587, 699, 1123, 1191, 1457, 1581
LmnI GCTCC 5 cut(s) 333, 388, 507, 1205, 1318
Lsp1109I GCAGC 4 cut(s) 27, 30, 149, 893
LweI GCATC 1 cut(s) 1333
MaeI CTAG 2 cut(s) 842, 975
MaeII ACGT 2 cut(s) 263, 659
MaeIII GTNAC 8 cut(s) 482, 629, 781, 1270, 1438, 1468, 1508, 1587
MalI GATC 8 cut(s) 315, 356, 589, 701, 1125, 1193, 1459, 1583
MboI GATC 8 cut(s) 313, 354, 587, 699, 1123, 1191, 1457, 1581
MboII GAAGA 4 cut(s) 33, 352, 416, 419
MfeI CAATTG 1 cut(s) 287
MflI RGATCY 3 cut(s) 313, 587, 1123
MhlI GDGCHC 7 cut(s) 147, 330, 813, 891, 999, 1063, 1383
MluCI AATT 4 cut(s) 287, 495, 546, 1266
MluI ACGCGT 1 cut(s) 417
MlyI GAGTC 6 cut(s) 58, 329, 350, 673, 721, 977
MmeI TCCRAC 2 cut(s) 615, 960
Mph1103I ATGCAT 2 cut(s) 1296, 1451
MreI CGCCGGCG 1 cut(s) 365
MroI TCCGGA 2 cut(s) 330, 682
MroNI GCCGGC 1 cut(s) 365
MseI TTAA 2 cut(s) 549, 1140
MslI CAYNNNNRTG 2 cut(s) 1199, 1220
MspA1I CMGCKG 2 cut(s) 161, 884
MspI CCGG 7 cut(s) 293, 331, 366, 683, 756, 850, 1176
MspR9I CCNGG 5 cut(s) 851, 1045, 1097, 1177, 1404
MunI CAATTG 1 cut(s) 287
Mva1269I GAATGC 3 cut(s) 238, 1367, 1451
MvaI CCWGG 3 cut(s) 1045, 1097, 1404
MvnI CGCG 2 cut(s) 419, 1492
NaeI GCCGGC 1 cut(s) 367
NciI CCSGG 2 cut(s) 851, 1177
NcoI CCATGG 1 cut(s) 408
NdeI CATATG 1 cut(s) 1020
NdeII GATC 8 cut(s) 313, 354, 587, 699, 1123, 1191, 1457, 1581
NgoMIV GCCGGC 1 cut(s) 365
NlaIII CATG 8 cut(s) 412, 877, 913, 941, 1219, 1278, 1298, 1320
NlaIV GGNNCC 7 cut(s) 186, 240, 687, 833, 1060, 1125, 1314
NmeAIII GCCGAG 3 cut(s) 166, 609, 651
NmuCI GTSAC 6 cut(s) 482, 1270, 1438, 1468, 1508, 1587
NsiI ATGCAT 2 cut(s) 1296, 1451
PagI TCATGA 2 cut(s) 873, 909
PasI CCCWGGG 1 cut(s) 1096
PctI GAATGC 3 cut(s) 238, 1367, 1451
PdiI GCCGGC 1 cut(s) 367
PfeI GAWTC 5 cut(s) 154, 665, 870, 1101, 1159
PinAI ACCGGT 1 cut(s) 755
PkrI GCNGC 8 cut(s) 17, 20, 139, 177, 208, 724, 769, 883
Ple19I CGATCG 1 cut(s) 357
PleI GAGTC 6 cut(s) 58, 328, 349, 673, 721, 977
PmaCI CACGTG 1 cut(s) 264
PmlI CACGTG 1 cut(s) 264
PpsI GAGTC 6 cut(s) 58, 328, 349, 673, 721, 977
Ppu21I YACGTR 1 cut(s) 264
Psp6I CCWGG 3 cut(s) 1043, 1095, 1402
PspCI CACGTG 1 cut(s) 264
PspFI CCCAGC 1 cut(s) 884
PspGI CCWGG 3 cut(s) 1043, 1095, 1402
PspN4I GGNNCC 7 cut(s) 186, 240, 687, 833, 1060, 1125, 1314
PspPI GGNCC 5 cut(s) 108, 117, 685, 862, 1429
PstI CTGCAG 1 cut(s) 883
PsuI RGATCY 3 cut(s) 313, 587, 1123
PvuI CGATCG 1 cut(s) 357
PvuII CAGCTG 2 cut(s) 161, 884
RsaI GTAC 2 cut(s) 299, 833
RsaNI GTAC 2 cut(s) 298, 832
RseI CAYNNNNRTG 2 cut(s) 1199, 1220
SalI GTCGAC 1 cut(s) 61
SaqAI TTAA 2 cut(s) 549, 1140
SatI GCNGC 8 cut(s) 16, 19, 138, 176, 207, 723, 768, 882
Sau3AI GATC 8 cut(s) 313, 354, 587, 699, 1123, 1191, 1457, 1581
Sau96I GGNCC 5 cut(s) 108, 117, 685, 862, 1429
SchI GAGTC 6 cut(s) 58, 329, 350, 673, 721, 977
ScrFI CCNGG 5 cut(s) 851, 1045, 1097, 1177, 1404
SduI GDGCHC 7 cut(s) 147, 330, 813, 891, 999, 1063, 1383
SfaNI GCATC 1 cut(s) 1333
SfcI CTRYAG 2 cut(s) 879, 1616
SfiI GGCCNNNNNGGCC 1 cut(s) 115
SgrAI CRCCGGYG 1 cut(s) 365
SinI GGWCC 1 cut(s) 685
SmiMI CAYNNNNRTG 2 cut(s) 1199, 1220
SmlI CTYRAG 2 cut(s) 1325, 1538
SmoI CTYRAG 2 cut(s) 1325, 1538
Sse9I AATT 4 cut(s) 287, 495, 546, 1266
SspI AATATT 1 cut(s) 478
SspMI CTAG 2 cut(s) 842, 975
StyD4I CCNGG 5 cut(s) 849, 1043, 1095, 1175, 1402
StyI CCWWGG 2 cut(s) 408, 865
TaaI ACNGT 7 cut(s) 184, 713, 895, 966, 1189, 1468, 1508
TaiI ACGT 2 cut(s) 266, 662
TaqI TCGA 3 cut(s) 62, 221, 1476
TasI AATT 4 cut(s) 287, 495, 546, 1266
TauI GCSGC 4 cut(s) 178, 209, 725, 770
TfiI GAWTC 5 cut(s) 154, 665, 870, 1101, 1159
Tru1I TTAA 2 cut(s) 549, 1140
Tru9I TTAA 2 cut(s) 549, 1140
TscAI CASTG 2 cut(s) 794, 1511
TseFI GTSAC 6 cut(s) 482, 1270, 1438, 1468, 1508, 1587
TseI GCWGC 4 cut(s) 15, 18, 137, 881
Tsp45I GTSAC 6 cut(s) 482, 1270, 1438, 1468, 1508, 1587
TspDTI ATGAA 5 cut(s) 37, 862, 975, 1403, 1540
TspGWI ACGGA 3 cut(s) 666, 1241, 1477
TspRI CASTG 2 cut(s) 794, 1511
VneI GTGCAC 1 cut(s) 1379
VpaK11BI GGWCC 1 cut(s) 685
XapI RAATTY 1 cut(s) 495
XmiI GTMKAC 1 cut(s) 62
XspI CTAG 2 cut(s) 842, 975
Zsp2I ATGCAT 2 cut(s) 1296, 1451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.