pycom08g16050

Phospho-2-dehydro-3-deoxyheptonate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0015832
Physical Location & Seq
Forward (+)
15942850 .. 15944187
1338 bp
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UTR
Exon/CDS
Intron
pycom08g16050.1

Sequence Viewer

Length: 420 bp
ATGGGAGCTGAGAACATGAGGGTGAAACTTCCTCATCTTATTAGGGCAGTCCGCAGGGCTGGACAGATTGTCACATGGGTCAGTGACCCAATGCATGGAAACACCATTAAGGCTCCCTGCGGCCTCAAAACACGTCCTTTCGATGCCATAAGGGCGGAGGTGAGAGCGTTCTTTGATGTGCACGAGCAAGAAGGAAGCCACCCAGGTGGTGTTCATCTAGAGATGACAGGCCAGAATGTGACAGAATGCATTGGAGGTTCACGCACTGTGACATTCGACGACCTTAGCTCACGCTACCACACTCACTGCGACCCAAGGCTCAACGCCTCACAATCTCTTGAGCTCGCCTTTATCATAGCCGAGCGCCTCAGGAAAAGAAGGATCAAGTCACAGAACCCGGTCGCCACTTTAGGTCTGTAG

Protein Analysis

140

Amino Acids

15.5

Weight (kDa)

9.56

Isoelectric Point (pI)

44.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAHP_synth_2 PF01474 1 - 120 4.5e-55 Class-II DAHP synthetase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22410
fragaria_vesca FvH4_5g36810
malus_domestica MD08G1186700.v1.1 MD15G1372600.v1.1
prunus_persica Prupe.1G521200_v2.0.a1
pyrus_communis pycom08g16050 pycom15g33360
rosa_chinensis RchiOBHm_Chr7g0243371
rosa_laevigata RLG00000000852
rosa_multiflora Rmu_sc0001036.1_g000028 Rmu_sc0001461.1_g000106
rosa_roxburghii Rroxscaffold_3G00222150
rosa_rugosa Rorug07G0318600
rosa_samantha Rh7BG444800
rosa_wichuraiana Rw7G039490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 95
AciI CCGC 3 cut(s) 52, 120, 155
AclWI GGATC 1 cut(s) 389
AfiI CCNNNNNNNGG 1 cut(s) 95
AflIII ACRYGT 1 cut(s) 131
AhdI GACNNNNNGTC 1 cut(s) 68
AjiI CACGTC 1 cut(s) 134
AjnI CCWGG 1 cut(s) 202
AleI CACNNNNGTG 1 cut(s) 204
AluBI AGCT 3 cut(s) 8, 288, 343
AluI AGCT 3 cut(s) 8, 288, 343
Alw21I GWGCWC 2 cut(s) 183, 345
Alw44I GTGCAC 1 cut(s) 179
AlwI GGATC 1 cut(s) 389
AoxI GGCC 2 cut(s) 121, 229
ApaLI GTGCAC 1 cut(s) 179
AspLEI GCGC 1 cut(s) 366
AsuC2I CCSGG 1 cut(s) 398
AsuHPI GGTGA 2 cut(s) 34, 172
AxyI CCTNAGG 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 183
BanII GRGCYC 1 cut(s) 345
BauI CACGAG 1 cut(s) 182
Bbv12I GWGCWC 2 cut(s) 183, 345
BciT130I CCWGG 1 cut(s) 204
BcnI CCSGG 1 cut(s) 398
BfaI CTAG 1 cut(s) 218
BfmI CTRYAG 1 cut(s) 416
BfoI RGCGCY 1 cut(s) 367
BglI GCCNNNNNGGC 1 cut(s) 152
BisI GCNGC 1 cut(s) 121
BlsI GCNGC 1 cut(s) 122
Bme1390I CCNGG 2 cut(s) 204, 398
BmeRI GACNNNNNGTC 1 cut(s) 68
BmgBI CACGTC 1 cut(s) 134
BmiI GGNNCC 1 cut(s) 114
BmrFI CCNGG 2 cut(s) 204, 398
BmsI GCATC 1 cut(s) 133
Bpu10I CCTNAGC 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 1 cut(s) 398
BsaJI CCNNGG 2 cut(s) 202, 314
Bsc4I CCNNNNNNNGG 1 cut(s) 95
Bse21I CCTNAGG 1 cut(s) 368
BseBI CCWGG 1 cut(s) 204
BseDI CCNNGG 2 cut(s) 202, 314
BseLI CCNNNNNNNGG 1 cut(s) 95
BseMII CTCAG 1 cut(s) 382
BseSI GKGCMC 1 cut(s) 183
Bsh1285I CGRYCG 1 cut(s) 402
BshFI GGCC 2 cut(s) 123, 231
BsiEI CGRYCG 1 cut(s) 402
BsiHKAI GWGCWC 2 cut(s) 183, 345
BsiSI CCGG 1 cut(s) 398
BslI CCNNNNNNNGG 1 cut(s) 95
BsmI GAATGC 1 cut(s) 251
BsnI GGCC 2 cut(s) 123, 231
Bsp1286I GDGCHC 2 cut(s) 183, 345
Bsp143I GATC 1 cut(s) 381
BspACI CCGC 3 cut(s) 52, 120, 155
BspANI GGCC 2 cut(s) 123, 231
BspCNI CTCAG 1 cut(s) 381
BspLI GGNNCC 1 cut(s) 114
BspPI GGATC 1 cut(s) 389
BssECI CCNNGG 2 cut(s) 202, 314
BssMI GATC 1 cut(s) 381
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 1 cut(s) 314
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 204
Bst4CI ACNGT 1 cut(s) 268
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 3 cut(s) 9, 284, 368
BstH2I RGCGCY 1 cut(s) 367
BstHHI GCGC 1 cut(s) 366
BstKTI GATC 1 cut(s) 384
BstMBI GATC 1 cut(s) 381
BstMCI CGRYCG 1 cut(s) 402
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstNI CCWGG 1 cut(s) 204
BstSCI CCNGG 2 cut(s) 202, 396
BstSFI CTRYAG 1 cut(s) 416
BstSLI GKGCMC 1 cut(s) 183
BstXI CCANNNNNNTGG 1 cut(s) 206
Bsu36I CCTNAGG 1 cut(s) 368
BsuRI GGCC 2 cut(s) 123, 231
BtrI CACGTC 1 cut(s) 134
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 3 cut(s) 88, 264, 304
Cac8I GCNNGC 1 cut(s) 345
CfoI GCGC 1 cut(s) 366
CviAII CATG 3 cut(s) 16, 75, 95
DdeI CTNAG 3 cut(s) 9, 284, 368
DpnI GATC 1 cut(s) 383
DpnII GATC 1 cut(s) 381
DriI GACNNNNNGTC 1 cut(s) 68
Eam1105I GACNNNNNGTC 1 cut(s) 68
EciI GGCGGA 1 cut(s) 170
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 314
Eco24I GRGCYC 1 cut(s) 345
Eco53kI GAGCTC 1 cut(s) 343
Eco81I CCTNAGG 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 343
EcoRII CCWGG 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 314
EcoT22I ATGCAT 2 cut(s) 96, 251
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 314
FaeI CATG 3 cut(s) 19, 78, 98
FaiI YATR 5 cut(s) 17, 76, 96, 149, 356
FatI CATG 3 cut(s) 15, 74, 94
Fnu4HI GCNGC 1 cut(s) 121
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 1 cut(s) 121
FspBI CTAG 1 cut(s) 218
GlaI GCGC 1 cut(s) 365
GluI GCNGC 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 367
HaeIII GGCC 2 cut(s) 123, 231
HapII CCGG 1 cut(s) 398
HhaI GCGC 1 cut(s) 366
Hin1II CATG 3 cut(s) 19, 78, 98
Hin6I GCGC 1 cut(s) 364
HinP1I GCGC 1 cut(s) 364
HpaII CCGG 1 cut(s) 398
HphI GGTGA 2 cut(s) 34, 172
Hpy166II GTNNAC 2 cut(s) 181, 260
Hpy188III TCNNGA 3 cut(s) 218, 338, 370
Hpy8I GTNNAC 2 cut(s) 181, 260
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 2 cut(s) 185, 372
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 1 cut(s) 133
HpyCH4V TGCA 3 cut(s) 94, 181, 249
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpyF3I CTNAG 3 cut(s) 9, 284, 368
HpySE526I ACGT 1 cut(s) 133
Hsp92II CATG 3 cut(s) 19, 78, 98
HspAI GCGC 1 cut(s) 364
Kzo9I GATC 1 cut(s) 381
LmnI GCTCC 2 cut(s) 5, 118
LpnPI CCDG 9 cut(s) 40, 45, 130, 189, 213, 216, 245, 355, 411
LweI GCATC 1 cut(s) 133
MaeI CTAG 1 cut(s) 218
MaeII ACGT 1 cut(s) 133
MaeIII GTNAC 5 cut(s) 70, 83, 238, 268, 387
MalI GATC 1 cut(s) 383
MboI GATC 1 cut(s) 381
MhlI GDGCHC 2 cut(s) 183, 345
MnlI CCTC 7 cut(s) 12, 42, 134, 151, 248, 337, 377
Mph1103I ATGCAT 2 cut(s) 96, 251
MseI TTAA 1 cut(s) 108
MslI CAYNNNNRTG 2 cut(s) 20, 204
MspI CCGG 1 cut(s) 398
MspR9I CCNGG 2 cut(s) 204, 398
Mva1269I GAATGC 1 cut(s) 251
MvaI CCWGG 1 cut(s) 204
MwoI GCNNNNNNNGC 1 cut(s) 152
NciI CCSGG 1 cut(s) 398
NdeII GATC 1 cut(s) 381
NlaIII CATG 3 cut(s) 19, 78, 98
NlaIV GGNNCC 1 cut(s) 114
NmeAIII GCCGAG 1 cut(s) 385
NmuCI GTSAC 5 cut(s) 70, 83, 238, 268, 387
NsiI ATGCAT 2 cut(s) 96, 251
OliI CACNNNNGTG 1 cut(s) 204
PctI GAATGC 1 cut(s) 251
PflMI CCANNNNNTGG 1 cut(s) 95
PkrI GCNGC 1 cut(s) 122
Psp124BI GAGCTC 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 202
PspGI CCWGG 1 cut(s) 202
PspN4I GGNNCC 1 cut(s) 114
RseI CAYNNNNRTG 2 cut(s) 20, 204
SacI GAGCTC 1 cut(s) 345
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 1 cut(s) 121
Sau3AI GATC 1 cut(s) 381
ScrFI CCNGG 2 cut(s) 204, 398
SduI GDGCHC 2 cut(s) 183, 345
SetI ASST 9 cut(s) 10, 136, 162, 208, 259, 285, 290, 345, 415
SfaNI GCATC 1 cut(s) 133
SfcI CTRYAG 1 cut(s) 416
SmiMI CAYNNNNRTG 2 cut(s) 20, 204
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
SsiI CCGC 3 cut(s) 52, 120, 155
SspMI CTAG 1 cut(s) 218
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 2 cut(s) 202, 396
StyI CCWWGG 1 cut(s) 314
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 1 cut(s) 136
TaqI TCGA 2 cut(s) 141, 276
TauI GCSGC 1 cut(s) 123
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 3 cut(s) 88, 271, 311
TseFI GTSAC 5 cut(s) 70, 83, 238, 268, 387
Tsp45I GTSAC 5 cut(s) 70, 83, 238, 268, 387
TspDTI ATGAA 1 cut(s) 203
TspRI CASTG 3 cut(s) 88, 271, 311
Van91I CCANNNNNTGG 1 cut(s) 95
VneI GTGCAC 1 cut(s) 179
XbaI TCTAGA 1 cut(s) 217
XspI CTAG 1 cut(s) 218
Zsp2I ATGCAT 2 cut(s) 96, 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.