Rw7G039490

Phospho-2-dehydro-3-deoxyheptonate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0015832
Physical Location & Seq
Reverse (-)
60814107 .. 60818251
4145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G039490.1

Sequence Viewer

Length: 1623 bp
ATGGCTCTCACGGGCAGCAGCTCAATCATTGCCCCCAAATCTTCCTTCATTCAAAGCCAGTCGACTCTTCTACCCTCCAAACCTCACCAACCTTCTCTCCCCGTCAGGCCCATCAGGCCCATCGTCGCCGTCCACGCAGCCGAGCCCGCCAAGAATCCAGCTGTGTCCACCAAGCAGCAGACGGTGCCAGCAGCTTCGCCGTCAACTGCGGCGTCTAGTGGTTCGAAGAGCTGGAATGCGGTGCCGGCGAAATGGAGTTTGGACACTTGGAAATCCAAGAAGGCGGTGCAATTGCCGGAGTACCCGAATAAGGAGGATCTGGAGTCGGTGCTCCGGACGATTGAGTCTTTCCCGCCGATCGTCTTCGCCGGCGAGGCTAGGAGTTTAGAGGAGCGTCTCGCTGAGGCTTCCATGGGAAACGCGTTTCTTCTTCAAGGTGGGGATTGTGCGGAGAGCTTCAAGGAGTTCAATGCCAATAATATTCGTGACACCTTCAGAATTCTGCTCCAGATGGGCGTCGTTTTGATGTTCGGAGGCCAAATGCCAGTAATTAAGGTGGGAAGGATGGCGGGTCAGTTTGCGAAGCCGAGATCCGACCCGTTTGAGGAGAAGGATGGTGTCAAGCTTCCCAGTTACAGAGGGGACAATGTGAACGGAGACACGTTTGATTCAAAGTCAAGGACTCCGGACCCTGAGAGATTGATCAGAGCCTACTGTCAATCTGCGGCGACTCTCAACCTTCTCAGGGCCTTCGCAACCGGTGGTTATGCGGCTATGCAGAGGGTTACCCAGTGGAACTTGGATTTCACAGAGCATAGCGAACAGGGTGACAGGTACCGAGAACTAGCTCACCGGGTTGATGAGGCCCTTGGATTCATGAATGCAGCTGGGCTCACAGTTGACCATCCTATCATGACAACCACTGAGTTCTGGACATCCCATGAGTGCTTGCTTTTGCCTTATGAACAGTCGCTCACTAGGTTGGACTCAACTTCTGGGCTCTACTATGATTGCTCTGCCCATATGCTTTGGGTCGGTGAGCGAACCAGGCAACTGGATGGTGCCCATATTGAGTTCCTAAGAGGTGTTGCTAATCCCCTGGGAATCAAGGTGAGTGATAAGATGGATCCCAATGAGCTTGTTAAGCTCATTGAGATTTTGAATCCTCAAAATAAAGCCGGGAGGATAACGGTGATCACAAGAATGGGAGCTGAGAACATGAGAGTGAAACTTCCTCATCTTATTAGGGCAGTCCGTAGGGCAGGACAAATTGTCACATGGGTCAGCGACCCTATGCATGGAAACACCATCAAGGCTCCATGTGGTCTCAAGACACGCCCTTTTGATGCGATAAGGGCTGAGGTCAGAGCATTCTTTGACGTGCACGACCAAGAAGGAAGCCACCCAGGAGGTGTTCATCTGGAGATGACGGGCCAGAATGTGACTGAATGCATTGGAGGATCACGGACTGTGACATTCGACGACCTTAGCTCGCGCTACCATACCCACTGTGACCCGAGGCTAAATGCCTCCCAATCTCTTGAGCTTGCCTTCATTATTGCTGAGCGCCTGAGGAGACGAAGGATCAAGTCACAACTCTCTGTTGCCTCTTTAGGTCTGTAG

Protein Analysis

540

Amino Acids

59.67

Weight (kDa)

8.72

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAHP_synth_2 PF01474 85 - 521 2.1e-204 Class-II DAHP synthetase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22410
fragaria_vesca FvH4_5g36810
malus_domestica MD08G1186700.v1.1 MD15G1372600.v1.1
prunus_persica Prupe.1G521200_v2.0.a1
pyrus_communis pycom08g16050 pycom15g33360
rosa_chinensis RchiOBHm_Chr7g0243371
rosa_laevigata RLG00000000852
rosa_multiflora Rmu_sc0001036.1_g000028 Rmu_sc0001461.1_g000106
rosa_roxburghii Rroxscaffold_3G00222150
rosa_rugosa Rorug07G0318600
rosa_samantha Rh7BG444800
rosa_wichuraiana Rw7G039490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 343
Acc65I GGTACC 1 cut(s) 834
AccB1I GGYRCC 4 cut(s) 184, 241, 834, 1061
AccI GTMKAC 1 cut(s) 62
AccII CGCG 2 cut(s) 422, 1495
AccIII TCCGGA 2 cut(s) 333, 685
AciI CCGC 9 cut(s) 147, 209, 239, 284, 353, 449, 569, 725, 770
AclWI GGATC 6 cut(s) 324, 585, 1121, 1134, 1468, 1592
AcsI RAATTY 1 cut(s) 498
AcuI CTGAAG 1 cut(s) 478
AcyI GRCGYC 2 cut(s) 212, 516
AfaI GTAC 2 cut(s) 302, 836
AfiI CCNNNNNNNGG 4 cut(s) 310, 604, 745, 1298
AflIII ACRYGT 2 cut(s) 420, 660
AgeI ACCGGT 1 cut(s) 758
AgsI TTSAA 6 cut(s) 53, 434, 460, 469, 672, 1162
AhdI GACNNNNNGTC 1 cut(s) 1271
AjiI CACGTC 1 cut(s) 1381
AjnI CCWGG 3 cut(s) 1046, 1098, 1405
AjuI GAANNNNNNNTTGG 4 cut(s) 29, 61, 242, 274
Alw21I GWGCWC 2 cut(s) 333, 1386
Alw26I GTCTC 4 cut(s) 401, 651, 1331, 1570
Alw44I GTGCAC 1 cut(s) 1382
AlwI GGATC 6 cut(s) 324, 585, 1121, 1134, 1468, 1592
Ama87I CYCGRG 1 cut(s) 1516
Aor13HI TCCGGA 2 cut(s) 333, 685
AoxI GGCC 6 cut(s) 107, 116, 535, 747, 864, 1432
ApaLI GTGCAC 1 cut(s) 1382
ApeKI GCWGC 6 cut(s) 15, 18, 137, 175, 191, 884
ApoI RAATTY 1 cut(s) 498
AsiGI ACCGGT 1 cut(s) 758
Asp718I GGTACC 1 cut(s) 834
AspLEI GCGC 2 cut(s) 1497, 1569
AspS9I GGNCC 6 cut(s) 108, 117, 688, 747, 865, 1432
AsuC2I CCSGG 2 cut(s) 854, 1180
AsuHPI GGTGA 6 cut(s) 77, 839, 842, 1049, 1123, 1204
AsuII TTCGAA 1 cut(s) 224
AvaI CYCGRG 1 cut(s) 1516
AvaII GGWCC 1 cut(s) 688
AxyI CCTNAGG 1 cut(s) 1571
BaeGI GKGCMC 2 cut(s) 1066, 1386
BamHI GGATCC 1 cut(s) 1126
BanI GGYRCC 4 cut(s) 184, 241, 834, 1061
BanII GRGCYC 3 cut(s) 147, 894, 1002
BbsI GAAGAC 1 cut(s) 355
Bbv12I GWGCWC 2 cut(s) 333, 1386
BbvCI CCTCAGC 2 cut(s) 402, 1359
BbvI GCAGC 6 cut(s) 27, 30, 149, 187, 203, 896
BccI CCATC 9 cut(s) 119, 128, 505, 559, 608, 912, 1052, 1117, 1316
BceAI ACGGC 2 cut(s) 113, 184
BciT130I CCWGG 3 cut(s) 1048, 1100, 1407
BclI TGATCA 2 cut(s) 702, 1194
BcnI CCSGG 2 cut(s) 854, 1180
BcoDI GTCTC 4 cut(s) 401, 651, 1331, 1570
BfaI CTAG 4 cut(s) 216, 378, 845, 978
BfmI CTRYAG 1 cut(s) 1619
BfoI RGCGCY 1 cut(s) 1570
BglI GCCNNNNNGGC 2 cut(s) 115, 374
BisI GCNGC 9 cut(s) 16, 19, 138, 176, 192, 210, 726, 771, 885
BlpI GCTNAGC 1 cut(s) 1563
BlsI GCNGC 9 cut(s) 17, 20, 139, 177, 193, 211, 727, 772, 886
Bme1390I CCNGG 5 cut(s) 854, 1048, 1100, 1180, 1407
Bme18I GGWCC 1 cut(s) 688
BmeRI GACNNNNNGTC 1 cut(s) 1271
BmeT110I CYCGRG 1 cut(s) 1516
BmgBI CACGTC 1 cut(s) 1381
BmgT120I GGNCC 6 cut(s) 108, 117, 688, 747, 865, 1432
BmiI GGNNCC 7 cut(s) 186, 243, 690, 836, 1063, 1128, 1317
BmrFI CCNGG 5 cut(s) 854, 1048, 1100, 1180, 1407
BmrI ACTGGG 2 cut(s) 624, 784
BmsI GCATC 1 cut(s) 1336
BmuI ACTGGG 2 cut(s) 624, 784
BpiI GAAGAC 1 cut(s) 355
BpmI CTGGAG 3 cut(s) 341, 491, 1442
Bpu10I CCTNAGC 3 cut(s) 402, 1359, 1487
Bpu1102I GCTNAGC 1 cut(s) 1563
Bpu14I TTCGAA 1 cut(s) 224
BpuEI CTTGAG 2 cut(s) 1313, 1562
BpuMI CCSGG 2 cut(s) 854, 1180
BsaHI GRCGYC 2 cut(s) 212, 516
BsaI GGTCTC 1 cut(s) 1331
BsaJI CCNNGG 6 cut(s) 411, 868, 1098, 1099, 1405, 1517
BsaWI WCCGGW 3 cut(s) 333, 685, 758
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 4 cut(s) 310, 604, 745, 1298
Bse118I RCCGGY 3 cut(s) 244, 368, 758
Bse1I ACTGG 5 cut(s) 58, 545, 630, 790, 1059
Bse21I CCTNAGG 1 cut(s) 1571
Bse3DI GCAATG 1 cut(s) 27
BseAI TCCGGA 2 cut(s) 333, 685
BseBI CCWGG 3 cut(s) 1048, 1100, 1407
BseDI CCNNGG 6 cut(s) 411, 868, 1098, 1099, 1405, 1517
BseGI GGATG 5 cut(s) 570, 619, 904, 935, 1063
BseLI CCNNNNNNNGG 4 cut(s) 310, 604, 745, 1298
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 8 cut(s) 393, 684, 757, 915, 1203, 1350, 1554, 1562
BseNI ACTGG 5 cut(s) 58, 545, 630, 790, 1059
BseRI GAGGAG 3 cut(s) 404, 620, 1588
BseSI GKGCMC 2 cut(s) 1066, 1386
BseXI GCAGC 6 cut(s) 27, 30, 149, 187, 203, 896
BseYI CCCAGC 1 cut(s) 887
Bsh1236I CGCG 2 cut(s) 422, 1495
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 6 cut(s) 109, 118, 537, 749, 866, 1434
BshNI GGYRCC 4 cut(s) 184, 241, 834, 1061
BshTI ACCGGT 1 cut(s) 758
BsiEI CGRYCG 1 cut(s) 360
BsiHKAI GWGCWC 2 cut(s) 333, 1386
BsiHKCI CYCGRG 1 cut(s) 1516
BsiSI CCGG 8 cut(s) 245, 296, 334, 369, 686, 759, 853, 1179
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 4 cut(s) 310, 604, 745, 1298
BsmAI GTCTC 4 cut(s) 401, 651, 1331, 1570
BsmBI CGTCTC 2 cut(s) 401, 1570
BsmFI GGGAC 1 cut(s) 656
BsmI GAATGC 4 cut(s) 241, 886, 1370, 1454
BsnI GGCC 6 cut(s) 109, 118, 537, 749, 866, 1434
Bso31I GGTCTC 1 cut(s) 1331
BsoBI CYCGRG 1 cut(s) 1516
Bsp119I TTCGAA 1 cut(s) 224
Bsp1286I GDGCHC 6 cut(s) 147, 333, 894, 1002, 1066, 1386
Bsp13I TCCGGA 2 cut(s) 333, 685
Bsp143I GATC 8 cut(s) 316, 357, 590, 702, 1126, 1194, 1460, 1584
Bsp1720I GCTNAGC 1 cut(s) 1563
Bsp19I CCATGG 1 cut(s) 411
BspACI CCGC 9 cut(s) 147, 209, 239, 284, 353, 449, 569, 725, 770
BspANI GGCC 6 cut(s) 109, 118, 537, 749, 866, 1434
BspCNI CTCAG 8 cut(s) 394, 685, 756, 916, 1204, 1351, 1555, 1563
BspEI TCCGGA 2 cut(s) 333, 685
BspFNI CGCG 2 cut(s) 422, 1495
BspHI TCATGA 2 cut(s) 876, 912
BspLI GGNNCC 7 cut(s) 186, 243, 690, 836, 1063, 1128, 1317
BspPI GGATC 6 cut(s) 324, 585, 1121, 1134, 1468, 1592
BspQI GCTCTTC 1 cut(s) 221
BspT104I TTCGAA 1 cut(s) 224
BspT107I GGYRCC 4 cut(s) 184, 241, 834, 1061
BspTNI GGTCTC 1 cut(s) 1331
BsrDI GCAATG 1 cut(s) 27
BsrFI RCCGGY 3 cut(s) 244, 368, 758
BsrI ACTGG 5 cut(s) 58, 545, 630, 790, 1059
BssAI RCCGGY 3 cut(s) 244, 368, 758
BssECI CCNNGG 6 cut(s) 411, 868, 1098, 1099, 1405, 1517
BssMI GATC 8 cut(s) 316, 357, 590, 702, 1126, 1194, 1460, 1584
BssNI GRCGYC 2 cut(s) 212, 516
BssT1I CCWWGG 2 cut(s) 411, 868
Bst2UI CCWGG 3 cut(s) 1048, 1100, 1407
Bst4CI ACNGT 7 cut(s) 184, 716, 898, 969, 1192, 1471, 1511
Bst6I CTCTTC 2 cut(s) 72, 221
BstACI GRCGYC 2 cut(s) 212, 516
BstAPI GCANNNNNTGC 1 cut(s) 184
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 7 cut(s) 147, 189, 246, 370, 950, 1493, 1548
BstDSI CCRYGG 1 cut(s) 411
BstEII GGTNACC 1 cut(s) 784
BstF5I GGATG 5 cut(s) 570, 619, 904, 935, 1063
BstFNI CGCG 2 cut(s) 422, 1495
BstH2I RGCGCY 1 cut(s) 1570
BstHHI GCGC 2 cut(s) 1497, 1569
BstKTI GATC 8 cut(s) 319, 360, 593, 705, 1129, 1197, 1463, 1587
BstMAI GTCTC 4 cut(s) 401, 651, 1331, 1570
BstMBI GATC 8 cut(s) 316, 357, 590, 702, 1126, 1194, 1460, 1584
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 9 cut(s) 115, 134, 146, 184, 245, 374, 1048, 1144, 1355
BstNI CCWGG 3 cut(s) 1048, 1100, 1407
BstPI GGTNACC 1 cut(s) 784
BstSCI CCNGG 5 cut(s) 852, 1046, 1098, 1178, 1405
BstSFI CTRYAG 1 cut(s) 1619
BstSLI GKGCMC 2 cut(s) 1066, 1386
BstUI CGCG 2 cut(s) 422, 1495
BstV1I GCAGC 6 cut(s) 27, 30, 149, 187, 203, 896
BstV2I GAAGAC 1 cut(s) 355
BstX2I RGATCY 3 cut(s) 316, 590, 1126
BstXI CCANNNNNNTGG 1 cut(s) 1054
BstYI RGATCY 3 cut(s) 316, 590, 1126
Bsu36I CCTNAGG 1 cut(s) 1571
BsuRI GGCC 6 cut(s) 109, 118, 537, 749, 866, 1434
BtgI CCRYGG 1 cut(s) 411
BtrI CACGTC 1 cut(s) 1381
BtsCI GGATG 5 cut(s) 570, 619, 904, 935, 1063
BtsIMutI CAGTG 3 cut(s) 797, 921, 1507
Cac8I GCNNGC 7 cut(s) 147, 189, 246, 370, 950, 1493, 1548
CciI TCATGA 2 cut(s) 876, 912
CfoI GCGC 2 cut(s) 1497, 1569
Cfr10I RCCGGY 3 cut(s) 244, 368, 758
Cfr13I GGNCC 6 cut(s) 108, 117, 688, 747, 865, 1432
CseI GACGC 3 cut(s) 201, 383, 505
Csp6I GTAC 2 cut(s) 301, 835
CspAI ACCGGT 1 cut(s) 758
CviAII CATG 8 cut(s) 412, 877, 913, 941, 1219, 1278, 1298, 1320
CviQI GTAC 2 cut(s) 301, 835
DpnI GATC 8 cut(s) 318, 359, 592, 704, 1128, 1196, 1462, 1586
DpnII GATC 8 cut(s) 316, 357, 590, 702, 1126, 1194, 1460, 1584
DrdI GACNNNNNNGTC 1 cut(s) 343
DriI GACNNNNNGTC 1 cut(s) 1271
DseDI GACNNNNNNGTC 1 cut(s) 343
Eam1104I CTCTTC 2 cut(s) 72, 221
Eam1105I GACNNNNNGTC 1 cut(s) 1271
EarI CTCTTC 2 cut(s) 72, 221
Eco130I CCWWGG 2 cut(s) 411, 868
Eco24I GRGCYC 3 cut(s) 147, 894, 1002
Eco31I GGTCTC 1 cut(s) 1331
Eco47I GGWCC 1 cut(s) 688
Eco57I CTGAAG 1 cut(s) 478
Eco81I CCTNAGG 1 cut(s) 1571
Eco88I CYCGRG 1 cut(s) 1516
Eco91I GGTNACC 1 cut(s) 784
EcoO109I RGGNCCY 2 cut(s) 747, 865
EcoO65I GGTNACC 1 cut(s) 784
EcoRI GAATTC 1 cut(s) 498
EcoRII CCWGG 3 cut(s) 1046, 1098, 1405
EcoT14I CCWWGG 2 cut(s) 411, 868
EcoT22I ATGCAT 2 cut(s) 1299, 1454
EcoT38I GRGCYC 3 cut(s) 147, 894, 1002
ErhI CCWWGG 2 cut(s) 411, 868
Esp3I CGTCTC 2 cut(s) 401, 1570
FaeI CATG 8 cut(s) 415, 880, 916, 944, 1222, 1281, 1301, 1323
FaqI GGGAC 1 cut(s) 656
FatI CATG 8 cut(s) 411, 876, 912, 940, 1218, 1277, 1297, 1319
FauI CCCGC 3 cut(s) 154, 360, 562
FauNDI CATATG 1 cut(s) 1023
FbaI TGATCA 2 cut(s) 702, 1194
FblI GTMKAC 1 cut(s) 62
Fnu4HI GCNGC 9 cut(s) 16, 19, 138, 176, 192, 210, 726, 771, 885
FokI GGATG 5 cut(s) 577, 626, 891, 922, 1070
FriOI GRGCYC 3 cut(s) 147, 894, 1002
Fsp4HI GCNGC 9 cut(s) 16, 19, 138, 176, 192, 210, 726, 771, 885
FspBI CTAG 4 cut(s) 216, 378, 845, 978
GlaI GCGC 2 cut(s) 1496, 1568
GluI GCNGC 9 cut(s) 16, 19, 138, 176, 192, 210, 726, 771, 885
GsaI CCCAGC 1 cut(s) 891
GsuI CTGGAG 3 cut(s) 341, 491, 1442
HaeII RGCGCY 1 cut(s) 1570
HaeIII GGCC 6 cut(s) 109, 118, 537, 749, 866, 1434
HapII CCGG 8 cut(s) 245, 296, 334, 369, 686, 759, 853, 1179
HgaI GACGC 3 cut(s) 201, 383, 505
HhaI GCGC 2 cut(s) 1497, 1569
Hin1I GRCGYC 2 cut(s) 212, 516
Hin1II CATG 8 cut(s) 415, 880, 916, 944, 1222, 1281, 1301, 1323
Hin6I GCGC 2 cut(s) 1495, 1567
HinP1I GCGC 2 cut(s) 1495, 1567
HincII GTYRAC 3 cut(s) 63, 204, 901
HindII GTYRAC 3 cut(s) 63, 204, 901
HindIII AAGCTT 1 cut(s) 623
HpaII CCGG 8 cut(s) 245, 296, 334, 369, 686, 759, 853, 1179
HphI GGTGA 6 cut(s) 77, 839, 842, 1049, 1123, 1204
Hpy166II GTNNAC 7 cut(s) 63, 133, 168, 204, 652, 901, 1384
Hpy188I TCNGA 5 cut(s) 497, 533, 595, 707, 1367
Hpy8I GTNNAC 7 cut(s) 63, 133, 168, 204, 652, 901, 1384
Hpy99I CGWCG 3 cut(s) 128, 521, 1484
HpyCH4III ACNGT 7 cut(s) 184, 716, 898, 969, 1192, 1471, 1511
HpyCH4IV ACGT 2 cut(s) 662, 1380
HpyCH4V TGCA 6 cut(s) 289, 778, 884, 1297, 1384, 1452
HpyF10VI GCNNNNNNNGC 9 cut(s) 115, 134, 146, 184, 245, 374, 1048, 1144, 1355
HpySE526I ACGT 2 cut(s) 662, 1380
Hsp92I GRCGYC 2 cut(s) 212, 516
Hsp92II CATG 8 cut(s) 415, 880, 916, 944, 1222, 1281, 1301, 1323
HspAI GCGC 2 cut(s) 1495, 1567
Kpn2I TCCGGA 2 cut(s) 333, 685
KpnI GGTACC 1 cut(s) 838
KroI GCCGGC 2 cut(s) 244, 368
KroNI GCCGGC 2 cut(s) 246, 370
Ksp22I TGATCA 2 cut(s) 702, 1194
Kzo9I GATC 8 cut(s) 316, 357, 590, 702, 1126, 1194, 1460, 1584
LguI GCTCTTC 1 cut(s) 221
LmnI GCTCC 5 cut(s) 336, 391, 510, 1208, 1321
Lsp1109I GCAGC 6 cut(s) 27, 30, 149, 187, 203, 896
LweI GCATC 1 cut(s) 1336
MaeI CTAG 4 cut(s) 216, 378, 845, 978
MaeII ACGT 2 cut(s) 662, 1380
MaeIII GTNAC 9 cut(s) 485, 632, 784, 827, 1273, 1441, 1471, 1511, 1590
MalI GATC 8 cut(s) 318, 359, 592, 704, 1128, 1196, 1462, 1586
MboI GATC 8 cut(s) 316, 357, 590, 702, 1126, 1194, 1460, 1584
MboII GAAGA 6 cut(s) 33, 59, 238, 355, 419, 422
MfeI CAATTG 1 cut(s) 290
MflI RGATCY 3 cut(s) 316, 590, 1126
MhlI GDGCHC 6 cut(s) 147, 333, 894, 1002, 1066, 1386
MluCI AATT 4 cut(s) 290, 498, 549, 1269
MluI ACGCGT 1 cut(s) 420
MlyI GAGTC 6 cut(s) 58, 332, 353, 676, 724, 980
MmeI TCCRAC 2 cut(s) 618, 963
Mph1103I ATGCAT 2 cut(s) 1299, 1454
MreI CGCCGGCG 1 cut(s) 368
MroI TCCGGA 2 cut(s) 333, 685
MroNI GCCGGC 2 cut(s) 244, 368
MseI TTAA 2 cut(s) 552, 1143
MslI CAYNNNNRTG 2 cut(s) 1202, 1223
MspA1I CMGCKG 2 cut(s) 161, 887
MspI CCGG 8 cut(s) 245, 296, 334, 369, 686, 759, 853, 1179
MspR9I CCNGG 5 cut(s) 854, 1048, 1100, 1180, 1407
MunI CAATTG 1 cut(s) 290
Mva1269I GAATGC 4 cut(s) 241, 886, 1370, 1454
MvaI CCWGG 3 cut(s) 1048, 1100, 1407
MvnI CGCG 2 cut(s) 422, 1495
MwoI GCNNNNNNNGC 9 cut(s) 115, 134, 146, 184, 245, 374, 1048, 1144, 1355
NaeI GCCGGC 2 cut(s) 246, 370
NciI CCSGG 2 cut(s) 854, 1180
NcoI CCATGG 1 cut(s) 411
NdeI CATATG 1 cut(s) 1023
NdeII GATC 8 cut(s) 316, 357, 590, 702, 1126, 1194, 1460, 1584
NgoMIV GCCGGC 2 cut(s) 244, 368
NlaIII CATG 8 cut(s) 415, 880, 916, 944, 1222, 1281, 1301, 1323
NlaIV GGNNCC 7 cut(s) 186, 243, 690, 836, 1063, 1128, 1317
NmeAIII GCCGAG 2 cut(s) 166, 612
NmuCI GTSAC 7 cut(s) 485, 827, 1273, 1441, 1471, 1511, 1590
NsiI ATGCAT 2 cut(s) 1299, 1454
NspV TTCGAA 1 cut(s) 224
PagI TCATGA 2 cut(s) 876, 912
PasI CCCWGGG 1 cut(s) 1099
PciSI GCTCTTC 1 cut(s) 221
PctI GAATGC 4 cut(s) 241, 886, 1370, 1454
PdiI GCCGGC 2 cut(s) 246, 370
PfeI GAWTC 5 cut(s) 154, 668, 873, 1104, 1162
PinAI ACCGGT 1 cut(s) 758
PkrI GCNGC 9 cut(s) 17, 20, 139, 177, 193, 211, 727, 772, 886
Ple19I CGATCG 1 cut(s) 360
PleI GAGTC 6 cut(s) 58, 331, 352, 676, 724, 980
PpsI GAGTC 6 cut(s) 58, 331, 352, 676, 724, 980
Psp6I CCWGG 3 cut(s) 1046, 1098, 1405
PspEI GGTNACC 1 cut(s) 784
PspFI CCCAGC 1 cut(s) 887
PspGI CCWGG 3 cut(s) 1046, 1098, 1405
PspN4I GGNNCC 7 cut(s) 186, 243, 690, 836, 1063, 1128, 1317
PspPI GGNCC 6 cut(s) 108, 117, 688, 747, 865, 1432
PsuI RGATCY 3 cut(s) 316, 590, 1126
PvuI CGATCG 1 cut(s) 360
PvuII CAGCTG 2 cut(s) 161, 887
RsaI GTAC 2 cut(s) 302, 836
RsaNI GTAC 2 cut(s) 301, 835
RseI CAYNNNNRTG 2 cut(s) 1202, 1223
SalI GTCGAC 1 cut(s) 61
SapI GCTCTTC 1 cut(s) 221
SaqAI TTAA 2 cut(s) 552, 1143
SatI GCNGC 9 cut(s) 16, 19, 138, 176, 192, 210, 726, 771, 885
Sau3AI GATC 8 cut(s) 316, 357, 590, 702, 1126, 1194, 1460, 1584
Sau96I GGNCC 6 cut(s) 108, 117, 688, 747, 865, 1432
SchI GAGTC 6 cut(s) 58, 332, 353, 676, 724, 980
ScrFI CCNGG 5 cut(s) 854, 1048, 1100, 1180, 1407
SduI GDGCHC 6 cut(s) 147, 333, 894, 1002, 1066, 1386
SfaNI GCATC 1 cut(s) 1336
SfcI CTRYAG 1 cut(s) 1619
SfiI GGCCNNNNNGGCC 1 cut(s) 115
SfuI TTCGAA 1 cut(s) 224
SgrAI CRCCGGYG 1 cut(s) 368
SinI GGWCC 1 cut(s) 688
SmiMI CAYNNNNRTG 2 cut(s) 1202, 1223
SmlI CTYRAG 2 cut(s) 1328, 1541
SmoI CTYRAG 2 cut(s) 1328, 1541
Sse9I AATT 4 cut(s) 290, 498, 549, 1269
SsiI CCGC 9 cut(s) 147, 209, 239, 284, 353, 449, 569, 725, 770
SspI AATATT 1 cut(s) 481
SspMI CTAG 4 cut(s) 216, 378, 845, 978
StyD4I CCNGG 5 cut(s) 852, 1046, 1098, 1178, 1405
StyI CCWWGG 2 cut(s) 411, 868
TaaI ACNGT 7 cut(s) 184, 716, 898, 969, 1192, 1471, 1511
TaiI ACGT 2 cut(s) 665, 1383
TaqI TCGA 3 cut(s) 62, 224, 1479
TasI AATT 4 cut(s) 290, 498, 549, 1269
TauI GCSGC 3 cut(s) 212, 728, 773
TfiI GAWTC 5 cut(s) 154, 668, 873, 1104, 1162
Tru1I TTAA 2 cut(s) 552, 1143
Tru9I TTAA 2 cut(s) 552, 1143
TscAI CASTG 3 cut(s) 797, 928, 1514
TseFI GTSAC 7 cut(s) 485, 827, 1273, 1441, 1471, 1511, 1590
TseI GCWGC 6 cut(s) 15, 18, 137, 175, 191, 884
Tsp45I GTSAC 7 cut(s) 485, 827, 1273, 1441, 1471, 1511, 1590
TspDTI ATGAA 6 cut(s) 37, 865, 893, 978, 1406, 1543
TspGWI ACGGA 3 cut(s) 669, 1244, 1480
TspRI CASTG 3 cut(s) 797, 928, 1514
VneI GTGCAC 1 cut(s) 1382
VpaK11BI GGWCC 1 cut(s) 688
XapI RAATTY 1 cut(s) 498
XmiI GTMKAC 1 cut(s) 62
XspI CTAG 4 cut(s) 216, 378, 845, 978
Zsp2I ATGCAT 2 cut(s) 1299, 1454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.