Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0008307

Physical Location & Seq

Genomic Coordinates Forward (+)

29110649 .. 29116354

Gene Structure 5706 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g39430.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2373 bp

ATGGCTTCCACCACCACCATGGCCTCACTCTCCCTTTTCCTCTTCATCCTTTTGCAAACAACTAGCAGCAAAGCAGCCGGCCAGTCTCCGACGTCGTCGTCATTCGCTTGCAACCCCAACGACGCAAGCACCAAGGACTTGCCGTTTTGCCGCGTAAAGTTGCCGATACATGTGAGAGTGAGGGACCTGATCGGCAGGCTGACGCTGCAAGAGAAGGTGAAGCTGCTAGTGAACAGTGCCAAGGCCGTGCCACGCCTTGGGATCAAAGACTACGAGTGGTGGTCGGAGGCACTTCATGGGGTCTCCAATGTTGGCCCCGGAACCAAGTTTGGCGGGGAGTTTCCGGGAGCTACCAGCTTCCCTCAGGTCATCACCACCGCTGCTTCCTTCAATGCTTCTCTTTGGGAAGCCATCGGACAGGCTGTGTCAGACGAAGCAAGAGCAATGTATAATGGAGGTATGGGCGGGCTCACGTATTGGAGTCCAAATGTGAACGTACTCAGAGACCCGAGATGGGGCCGTGGACAGGAAACTCCCGGTGAAGATCCGGTGGTGGTCGGGGCGTATGCTGCCAGCTACGTCAGGGGTCTACAGGGAAATGCCGGCGGAGGGCACCAGTTGAAGGTGGCCGCTTGCTGTAAACACTTCACGGCTTATGACCTTGATAATTGGCATGGAGTTGATCGATTTCACTTCAACGCTAGGGTTAGCAAGCAGGACATGGAGGACACATTCGATGTTCCTTTCAGAATGTGCGTCAAAGAAGGAAAGGTGGCCAGTGTTATGTGCTCTTACAATCAGGTCAATGGTGTCCCAACCTGTGCAGACCCTAATCTCCTCAAAAAAACTGTACGTGCACAATGGGGGCTTGATGGCTACATTGTCTCTGATTGCGATTCAGTGGGGGTTTTCTATGATAACCAACACTACACATCAACACCAGAGGAAGCAGCTGCTGTTGCCCTCAAAGCAGGTTTGGATTTGGACTGTGGCCCATTCCTGGGGTTGCACACTGAAGATGCAGTGAAGAAGGGTTTGTTGAGTGAGGTTGATCATATTGATCTTGCATTGGCTAATACACTCACAGTCCAAATGAGACTGGGCATGTTTGATGGATCCGCTCACCCTTATGCTAATTTGGGTCCGAGGGATGTCTGCAGCCCTGCTCACCAGCAACTCGCTCTTGACGCTGCCACCCAAGGCATTGTTCTTCTCAAGAACCATGGCACCTCCCTGCCTTTATCCACCCGCCGCCACCGCACTGTAGCAGTCATTGGTCCCAACTCTGATGTTACTGTTACTATGATCGGTAACTATGCCGGTGTCGCTTGTGGTTACACCACACCCCTACAAGGAATAGGAAAATACAGCAGGACAGTTCACCAGCAGGGCTGCAGCAATGTAGCTTGCAAAGAAGACACATTGTTTGGTGCAGCCATTGATGCAGCTCGCCAAGCCGATGCGACCATTTTAGTAATGGGCCTCGACCAGTCCATCGAAGCAGAGTTCAGAGACCGAGTCGGCCTACTTTTACCTGGACGACAACAAGAGCTCATCTCTAAAGTTGCTGCATCTTCCAAAGGCCCAACCATTTTGGTCTTGATGTCTGGCGGCCCAGTTGATGTTACTTTTGCCAAGAATGACCCACATATCGCTGGCATTGTATGGGCCGGATACCCAGGACAAGCTGGTGGACAAGCCATTGCTGATATTGTGTTTGGAATATCAAATCCAGGAGGAAAGCTGCCTATGACATGGTACCCGGAGGAGTATCTTAGAAATCTGGAAATGACAGACATGGGGATGAGGTCAAGGCCATCAAAAGGGTACCCTGGAAGAACCTACAGATTCTACAAAGGGCCAATTGTGTACCCATTTGGACATGGATTGGGGTACACTAAATTTGTGCAAACCGTAGCGAGCGCACCCACCGTAGTTGCAGTCCCTCTTGATGGTCATCATACTGAGACTAGTACCAGTGCCGGTAATGGCACCGGTACTAGGCCATTCGCAGCTGACGGGAAGGCAATTCGCGTGACACATGCAAAATGTAGCAAGCTTTCACTAGTTGTTGATGTGGATGTGAGAAATGTTGGGAACAGAGACGGGTCTCACACTTTGCTAGTGTACTCTAGTCCTCCGGCCGCTGCCGGACACTGGGTGCCACACAAACAATTGGTTGCCTTTGAGAAAGTATATGTTCCGGCCAGGACTCAACGGCGAGTCCGATTCAACATTCATGTGTGCAAGCACCTTAGTGTGGTGGACAGGTCTGGGATTCGAAGAATTCCAATGGGACGTCACGACCTTCACATTGGTGATCATATCGTCCACTCTTTCTCCATCCAAGCCGCTTCAATCATCAAATCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

791

Amino Acids

84.98

Weight (kDa)

8.72

Isoelectric Point (pI)

34.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	114 - 329	3.3e-37	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	402 - 578	2.2e-40	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008307)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02640
fragaria_vesca	FvH4_5g39430 FvH4_5g39430 FvH4_5g39430 FvH4_5g39430
malus_domestica	MD03G1296500.v1.1 MD11G1315900.v1.1
prunus_persica	Prupe.8G270800_v2.0.a1 Prupe.8G270800_v2.0.a1
pyrus_communis	pycom03g23490 pycom11g27580
rosa_chinensis	RchiOBHm_Chr7g0241741
rosa_laevigata	RLG00000000548
rosa_multiflora	Rmu_co8482979.1_g000001 Rmu_sc0010736.1_g000003 Rmu_sc0029163.1_g000002
rosa_roxburghii	Rroxscaffold_3G00218710
rosa_rugosa	Rorug07G0346900
rosa_samantha	Rh7AG501300 Rh7BG472600 Rh7CG517600 Rh7DG486300
rosa_wichuraiana	Rw7G042370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	97
AatII	GACGTC	2 cut(s)	95, 2302
Acc36I	ACCTGC	1 cut(s)	962
Acc65I	GGTACC	2 cut(s)	1758, 1827
AccB1I	GGYRCC	6 cut(s)	612, 1226, 1758, 1827, 1991, 2161
AccB7I	CCANNNNNTGG	1 cut(s)	257
AccBSI	CCGCTC	1 cut(s)	1121
AccI	GTMKAC	1 cut(s)	589
AccII	CGCG	2 cut(s)	153, 2034
AclWI	GGATC	4 cut(s)	269, 539, 1110, 1123
AcoI	YGGCCR	5 cut(s)	79, 627, 774, 2142, 2205
AcsI	RAATTY	2 cut(s)	1901, 2286
AcuI	CTGAAG	1 cut(s)	1035
AcyI	GRCGYC	2 cut(s)	92, 2299
AdeI	CACNNNGTG	1 cut(s)	2161
AfaI	GTAC	9 cut(s)	498, 852, 1760, 1829, 1871, 1895, 1975, 1999, 2129
AflIII	ACRYGT	1 cut(s)	169
AgeI	ACCGGT	1 cut(s)	1994
AgsI	TTSAA	5 cut(s)	391, 622, 697, 2233, 2358
AhlI	ACTAGT	2 cut(s)	1970, 2065
AjnI	CCWGG	6 cut(s)	999, 1534, 1678, 1732, 1831, 2207
AjuI	GAANNNNNNNTTGG	2 cut(s)	1191, 1223
AleI	CACNNNNGTG	2 cut(s)	2256, 2316
Alw21I	GWGCWC	3 cut(s)	791, 859, 1554
Alw26I	GTCTC	9 cut(s)	90, 307, 498, 889, 1090, 1506, 1961, 2097, 2115
Alw44I	GTGCAC	1 cut(s)	855
AlwI	GGATC	4 cut(s)	269, 539, 1110, 1123
AlwNI	CAGNNNCTG	1 cut(s)	956
Ama87I	CYCGRG	1 cut(s)	508
ApaLI	GTGCAC	1 cut(s)	855
ApoI	RAATTY	2 cut(s)	1901, 2286
ArsI	GACNNNNNNTTYG	2 cut(s)	1409, 1441
AsiGI	ACCGGT	1 cut(s)	1994
Asp718I	GGTACC	2 cut(s)	1758, 1827
AspLEI	GCGC	1 cut(s)	1925
AsuC2I	CCSGG	4 cut(s)	318, 345, 537, 1763
AsuHPI	GGTGA	7 cut(s)	229, 364, 551, 1115, 1160, 1373, 2330
AsuII	TTCGAA	1 cut(s)	2281
AvaI	CYCGRG	1 cut(s)	508
AvaII	GGWCC	3 cut(s)	184, 1142, 1277
AxyI	CCTNAGG	1 cut(s)	363
BaeGI	GKGCMC	2 cut(s)	615, 859
BalI	TGGCCA	1 cut(s)	776
BamHI	GGATCC	1 cut(s)	1115
BanI	GGYRCC	6 cut(s)	612, 1226, 1758, 1827, 1991, 2161
BanII	GRGCYC	2 cut(s)	471, 1554
BbsI	GAAGAC	1 cut(s)	1422
Bbv12I	GWGCWC	3 cut(s)	791, 859, 1554
BccI	CCATC	8 cut(s)	419, 507, 866, 1106, 1502, 1825, 1946, 2351
BceAI	ACGGC	5 cut(s)	127, 230, 504, 666, 2234
BciT130I	CCWGG	6 cut(s)	1001, 1536, 1680, 1734, 1833, 2209
BciVI	GTATCC	1 cut(s)	1667
BclI	TGATCA	2 cut(s)	1051, 2320
BcnI	CCSGG	4 cut(s)	318, 345, 537, 1763
BcoDI	GTCTC	9 cut(s)	90, 307, 498, 889, 1090, 1506, 1961, 2097, 2115
BcuI	ACTAGT	2 cut(s)	1970, 2065
BfaI	CTAG	8 cut(s)	63, 227, 702, 1971, 2001, 2066, 2123, 2133
BfmI	CTRYAG	5 cut(s)	590, 1156, 1263, 1393, 1843
BfuAI	ACCTGC	1 cut(s)	962
BfuI	GTATCC	1 cut(s)	1667
Bme18I	GGWCC	3 cut(s)	184, 1142, 1277
BmeT110I	CYCGRG	1 cut(s)	508
BmrI	ACTGGG	3 cut(s)	1109, 1610, 2167
BmsI	GCATC	4 cut(s)	1009, 1432, 1450, 1580
BmuI	ACTGGG	3 cut(s)	1109, 1610, 2167
BpiI	GAAGAC	1 cut(s)	1422
BplI	GAGNNNNNCTC	2 cut(s)	1541, 1573
Bpu14I	TTCGAA	1 cut(s)	2281
BpuEI	CTTGAG	1 cut(s)	1199
BpuMI	CCSGG	4 cut(s)	318, 345, 537, 1763
Bsa29I	ATCGAT	1 cut(s)	685
BsaAI	YACGTR	2 cut(s)	474, 854
BsaBI	GATNNNNATC	1 cut(s)	1956
BsaHI	GRCGYC	2 cut(s)	92, 2299
BsaI	GGTCTC	4 cut(s)	307, 498, 1506, 2115
BsaWI	WCCGGW	2 cut(s)	547, 1994
BsaXI	ACNNNNNCTCC	2 cut(s)	2324, 2354
Bse118I	RCCGGY	5 cut(s)	77, 602, 1319, 1982, 1994
Bse1I	ACTGG	8 cut(s)	82, 616, 777, 1104, 1489, 1616, 1977, 2162
Bse21I	CCTNAGG	1 cut(s)	363
Bse3DI	GCAATG	3 cut(s)	450, 1405, 1701
Bse8I	GATNNNNATC	1 cut(s)	1956
BseBI	CCWGG	6 cut(s)	1001, 1536, 1680, 1734, 1833, 2209
BseCI	ATCGAT	1 cut(s)	685
BseGI	GGATG	5 cut(s)	45, 1156, 1809, 2086, 2343
BseJI	GATNNNNATC	1 cut(s)	1956
BseMI	GCAATG	3 cut(s)	450, 1405, 1701
BseMII	CTCAG	3 cut(s)	377, 514, 1956
BseNI	ACTGG	8 cut(s)	82, 616, 777, 1104, 1489, 1616, 1977, 2162
BseRI	GAGGAG	2 cut(s)	827, 1781
BseSI	GKGCMC	2 cut(s)	615, 859
BseX3I	CGGCCG	1 cut(s)	2142
BsgI	GTGCAG	2 cut(s)	843, 1452
Bsh1236I	CGCG	2 cut(s)	153, 2034
Bsh1285I	CGRYCG	1 cut(s)	2145
BshNI	GGYRCC	6 cut(s)	612, 1226, 1758, 1827, 1991, 2161
BshTI	ACCGGT	1 cut(s)	1994
BshVI	ATCGAT	1 cut(s)	685
BsiEI	CGRYCG	1 cut(s)	2145
BsiHKAI	GWGCWC	3 cut(s)	791, 859, 1554
BsiHKCI	CYCGRG	1 cut(s)	508
BslFI	GGGAC	5 cut(s)	197, 797, 1263, 1928, 2310
BsmAI	GTCTC	9 cut(s)	90, 307, 498, 889, 1090, 1506, 1961, 2097, 2115
BsmBI	CGTCTC	1 cut(s)	2097
BsmFI	GGGAC	5 cut(s)	197, 797, 1263, 1928, 2310
Bso31I	GGTCTC	4 cut(s)	307, 498, 1506, 2115
BsoBI	CYCGRG	1 cut(s)	508
Bsp119I	TTCGAA	1 cut(s)	2281
Bsp1286I	GDGCHC	5 cut(s)	471, 615, 791, 859, 1554
Bsp143I	GATC	9 cut(s)	189, 261, 544, 682, 1051, 1060, 1115, 1305, 2320
Bsp19I	CCATGG	2 cut(s)	18, 1222
BspCNI	CTCAG	3 cut(s)	376, 513, 1957
BspDI	ATCGAT	1 cut(s)	685
BspFNI	CGCG	2 cut(s)	153, 2034
BspMAI	CTGCAG	2 cut(s)	1160, 1397
BspMI	ACCTGC	1 cut(s)	962
BspPI	GGATC	4 cut(s)	269, 539, 1110, 1123
BspT104I	TTCGAA	1 cut(s)	2281
BspT107I	GGYRCC	6 cut(s)	612, 1226, 1758, 1827, 1991, 2161
BspTNI	GGTCTC	4 cut(s)	307, 498, 1506, 2115
BsrBI	CCGCTC	1 cut(s)	1121
BsrDI	GCAATG	3 cut(s)	450, 1405, 1701
BsrFI	RCCGGY	5 cut(s)	77, 602, 1319, 1982, 1994
BsrI	ACTGG	8 cut(s)	82, 616, 777, 1104, 1489, 1616, 1977, 2162
BssAI	RCCGGY	5 cut(s)	77, 602, 1319, 1982, 1994
BssMI	GATC	9 cut(s)	189, 261, 544, 682, 1051, 1060, 1115, 1305, 2320
BssNI	GRCGYC	2 cut(s)	92, 2299
BssT1I	CCWWGG	6 cut(s)	18, 132, 240, 256, 1198, 1222
Bst2UI	CCWGG	6 cut(s)	1001, 1536, 1680, 1734, 1833, 2209
Bst4CI	ACNGT	9 cut(s)	236, 850, 989, 1087, 1264, 1297, 1378, 1915, 1933
Bst6I	CTCTTC	1 cut(s)	47
BstACI	GRCGYC	2 cut(s)	92, 2299
BstBAI	YACGTR	2 cut(s)	474, 854
BstBI	TTCGAA	1 cut(s)	2281
BstDEI	CTNAG	5 cut(s)	363, 500, 1775, 1965, 2255
BstDSI	CCRYGG	3 cut(s)	18, 520, 1222
BstF5I	GGATG	5 cut(s)	45, 1156, 1809, 2086, 2343
BstFNI	CGCG	2 cut(s)	153, 2034
BstHHI	GCGC	1 cut(s)	1925
BstKTI	GATC	9 cut(s)	192, 264, 547, 685, 1054, 1063, 1118, 1308, 2323
BstMAI	GTCTC	9 cut(s)	90, 307, 498, 889, 1090, 1506, 1961, 2097, 2115
BstMBI	GATC	9 cut(s)	189, 261, 544, 682, 1051, 1060, 1115, 1305, 2320
BstMCI	CGRYCG	1 cut(s)	2145
BstMWI	GCNNNNNNNGC	9 cut(s)	205, 569, 959, 968, 1187, 1257, 1325, 1442, 1454
BstNI	CCWGG	6 cut(s)	1001, 1536, 1680, 1734, 1833, 2209
BstNSI	RCATGY	3 cut(s)	173, 1108, 2045
BstSFI	CTRYAG	5 cut(s)	590, 1156, 1263, 1393, 1843
BstSLI	GKGCMC	2 cut(s)	615, 859
BstUI	CGCG	2 cut(s)	153, 2034
BstV2I	GAAGAC	1 cut(s)	1422
BstX2I	RGATCY	2 cut(s)	544, 1115
BstXI	CCANNNNNNTGG	1 cut(s)	19
BstYI	RGATCY	2 cut(s)	544, 1115
BstZI	CGGCCG	1 cut(s)	2142
Bsu15I	ATCGAT	1 cut(s)	685
Bsu36I	CCTNAGG	1 cut(s)	363
BsuI	GTATCC	1 cut(s)	1667
BsuTUI	ATCGAT	1 cut(s)	685
BtgI	CCRYGG	3 cut(s)	18, 520, 1222
BtsCI	GGATG	5 cut(s)	45, 1156, 1809, 2086, 2343
BtsI	GCAGTG	1 cut(s)	1029
BtsIMutI	CAGTG	8 cut(s)	241, 784, 906, 1011, 1029, 1260, 1984, 2155
BveI	ACCTGC	1 cut(s)	962
CaiI	CAGNNNCTG	1 cut(s)	956
CfoI	GCGC	1 cut(s)	1925
Cfr10I	RCCGGY	5 cut(s)	77, 602, 1319, 1982, 1994
ClaI	ATCGAT	1 cut(s)	685
CseI	GACGC	4 cut(s)	131, 211, 745, 1196
Csp6I	GTAC	9 cut(s)	497, 851, 1759, 1828, 1870, 1894, 1974, 1998, 2128
CspAI	ACCGGT	1 cut(s)	1994
CspCI	CAANNNNNGTGG	2 cut(s)	1918, 1953
CviQI	GTAC	9 cut(s)	497, 851, 1759, 1828, 1870, 1894, 1974, 1998, 2128
DdeI	CTNAG	5 cut(s)	363, 500, 1775, 1965, 2255
DpnI	GATC	9 cut(s)	191, 263, 546, 684, 1053, 1062, 1117, 1307, 2322
DpnII	GATC	9 cut(s)	189, 261, 544, 682, 1051, 1060, 1115, 1305, 2320
DraIII	CACNNNGTG	1 cut(s)	2161
DrdI	GACNNNNNNGTC	1 cut(s)	97
DseDI	GACNNNNNNGTC	1 cut(s)	97
EaeI	YGGCCR	5 cut(s)	79, 627, 774, 2142, 2205
EagI	CGGCCG	1 cut(s)	2142
Eam1104I	CTCTTC	1 cut(s)	47
EarI	CTCTTC	1 cut(s)	47
EciI	GGCGGA	1 cut(s)	621
Ecl136II	GAGCTC	1 cut(s)	1552
EclXI	CGGCCG	1 cut(s)	2142
Eco130I	CCWWGG	6 cut(s)	18, 132, 240, 256, 1198, 1222
Eco24I	GRGCYC	2 cut(s)	471, 1554
Eco31I	GGTCTC	4 cut(s)	307, 498, 1506, 2115
Eco47I	GGWCC	3 cut(s)	184, 1142, 1277
Eco52I	CGGCCG	1 cut(s)	2142
Eco53kI	GAGCTC	1 cut(s)	1552
Eco57I	CTGAAG	1 cut(s)	1035
Eco81I	CCTNAGG	1 cut(s)	363
Eco88I	CYCGRG	1 cut(s)	508
EcoICRI	GAGCTC	1 cut(s)	1552
EcoO109I	RGGNCCY	1 cut(s)	184
EcoRI	GAATTC	1 cut(s)	2286
EcoRII	CCWGG	6 cut(s)	999, 1534, 1678, 1732, 1831, 2207
EcoT14I	CCWWGG	6 cut(s)	18, 132, 240, 256, 1198, 1222
EcoT38I	GRGCYC	2 cut(s)	471, 1554
ErhI	CCWWGG	6 cut(s)	18, 132, 240, 256, 1198, 1222
Esp3I	CGTCTC	1 cut(s)	2097
FaqI	GGGAC	5 cut(s)	197, 797, 1263, 1928, 2310
FauI	CCCGC	3 cut(s)	326, 458, 1256
FbaI	TGATCA	2 cut(s)	1051, 2320
FblI	GTMKAC	1 cut(s)	589
FokI	GGATG	5 cut(s)	32, 1163, 1816, 2093, 2330
FriOI	GRGCYC	2 cut(s)	471, 1554
FspBI	CTAG	8 cut(s)	63, 227, 702, 1971, 2001, 2066, 2123, 2133
GlaI	GCGC	1 cut(s)	1924
HgaI	GACGC	4 cut(s)	131, 211, 745, 1196
HhaI	GCGC	1 cut(s)	1925
Hin1I	GRCGYC	2 cut(s)	92, 2299
Hin6I	GCGC	1 cut(s)	1923
HinP1I	GCGC	1 cut(s)	1923
HindIII	AAGCTT	1 cut(s)	2057
HinfI	GANTC	8 cut(s)	481, 896, 1518, 1848, 2212, 2223, 2229, 2278
HphI	GGTGA	7 cut(s)	229, 364, 551, 1115, 1160, 1373, 2330
Hpy188III	TCNNGA	7 cut(s)	1184, 1216, 1600, 1784, 1949, 2303, 2370
Hpy99I	CGWCG	4 cut(s)	94, 97, 100, 125
HpyAV	CCTTC	7 cut(s)	208, 397, 616, 758, 1024, 2017, 2318
HpyCH4III	ACNGT	9 cut(s)	236, 850, 989, 1087, 1264, 1297, 1378, 1915, 1933
HpyCH4IV	ACGT	6 cut(s)	92, 473, 495, 579, 853, 2299
HpyF10VI	GCNNNNNNNGC	9 cut(s)	205, 569, 959, 968, 1187, 1257, 1325, 1442, 1454
HpyF3I	CTNAG	5 cut(s)	363, 500, 1775, 1965, 2255
HpySE526I	ACGT	6 cut(s)	92, 473, 495, 579, 853, 2299
Hsp92I	GRCGYC	2 cut(s)	92, 2299
HspAI	GCGC	1 cut(s)	1923
KpnI	GGTACC	2 cut(s)	1762, 1831
KroI	GCCGGC	2 cut(s)	77, 602
KroNI	GCCGGC	2 cut(s)	79, 604
Ksp22I	TGATCA	2 cut(s)	1051, 2320
Kzo9I	GATC	9 cut(s)	189, 261, 544, 682, 1051, 1060, 1115, 1305, 2320
LmnI	GCTCC	1 cut(s)	347
LweI	GCATC	4 cut(s)	1009, 1432, 1450, 1580
MaeI	CTAG	8 cut(s)	63, 227, 702, 1971, 2001, 2066, 2123, 2133
MaeII	ACGT	6 cut(s)	92, 473, 495, 579, 853, 2299
MaeIII	GTNAC	7 cut(s)	1291, 1297, 1310, 1334, 1624, 2035, 2300
MalI	GATC	9 cut(s)	191, 263, 546, 684, 1053, 1062, 1117, 1307, 2322
MbiI	CCGCTC	1 cut(s)	1121
MboI	GATC	9 cut(s)	189, 261, 544, 682, 1051, 1060, 1115, 1305, 2320
MboII	GAAGA	9 cut(s)	34, 554, 1028, 1039, 1202, 1427, 1566, 1848, 2295
MfeI	CAATTG	2 cut(s)	1863, 2174
MflI	RGATCY	2 cut(s)	544, 1115
MhlI	GDGCHC	5 cut(s)	471, 615, 791, 859, 1554
MlsI	TGGCCA	1 cut(s)	776
MluCI	AATT	7 cut(s)	667, 1135, 1863, 1901, 2028, 2174, 2286
MluNI	TGGCCA	1 cut(s)	776
MlyI	GAGTC	4 cut(s)	490, 1527, 2206, 2232
MmeI	TCCRAC	2 cut(s)	113, 264
Mox20I	TGGCCA	1 cut(s)	776
MroNI	GCCGGC	2 cut(s)	77, 602
MscI	TGGCCA	1 cut(s)	776
MslI	CAYNNNNRTG	6 cut(s)	17, 1128, 1802, 2240, 2256, 2316
Msp20I	TGGCCA	1 cut(s)	776
MspA1I	CMGCKG	4 cut(s)	380, 953, 2015, 2147
MunI	CAATTG	2 cut(s)	1863, 2174
MvaI	CCWGG	6 cut(s)	1001, 1536, 1680, 1734, 1833, 2209
MvnI	CGCG	2 cut(s)	153, 2034
MwoI	GCNNNNNNNGC	9 cut(s)	205, 569, 959, 968, 1187, 1257, 1325, 1442, 1454
NaeI	GCCGGC	2 cut(s)	79, 604
NciI	CCSGG	4 cut(s)	318, 345, 537, 1763
NcoI	CCATGG	2 cut(s)	18, 1222
NdeII	GATC	9 cut(s)	189, 261, 544, 682, 1051, 1060, 1115, 1305, 2320
NgoMIV	GCCGGC	2 cut(s)	77, 602
NmuCI	GTSAC	2 cut(s)	2035, 2300
NspI	RCATGY	3 cut(s)	173, 1108, 2045
NspV	TTCGAA	1 cut(s)	2281
OliI	CACNNNNGTG	2 cut(s)	2256, 2316
PciI	ACATGT	1 cut(s)	169
PcsI	WCGNNNNNNNCGW	1 cut(s)	2224
PdiI	GCCGGC	2 cut(s)	79, 604
PfeI	GAWTC	4 cut(s)	896, 1848, 2229, 2278
PflFI	GACNNNGTC	2 cut(s)	94, 1517
PflMI	CCANNNNNTGG	1 cut(s)	257
PfoI	TCCNGGA	2 cut(s)	343, 1732
PinAI	ACCGGT	1 cut(s)	1994
PleI	GAGTC	4 cut(s)	489, 1526, 2206, 2231
PpsI	GAGTC	4 cut(s)	489, 1526, 2206, 2231
Ppu21I	YACGTR	2 cut(s)	474, 854
PpuMI	RGGWCCY	1 cut(s)	184
PscI	ACATGT	1 cut(s)	169
Psp124BI	GAGCTC	1 cut(s)	1554
Psp5II	RGGWCCY	1 cut(s)	184
Psp6I	CCWGG	6 cut(s)	999, 1534, 1678, 1732, 1831, 2207
PspGI	CCWGG	6 cut(s)	999, 1534, 1678, 1732, 1831, 2207
PspPPI	RGGWCCY	1 cut(s)	184
PstI	CTGCAG	2 cut(s)	1160, 1397
PstNI	CAGNNNCTG	1 cut(s)	956
PsuI	RGATCY	2 cut(s)	544, 1115
PsyI	GACNNNGTC	2 cut(s)	94, 1517
PvuII	CAGCTG	2 cut(s)	953, 2015
RsaI	GTAC	9 cut(s)	498, 852, 1760, 1829, 1871, 1895, 1975, 1999, 2129
RsaNI	GTAC	9 cut(s)	497, 851, 1759, 1828, 1870, 1894, 1974, 1998, 2128
RseI	CAYNNNNRTG	6 cut(s)	17, 1128, 1802, 2240, 2256, 2316
SacI	GAGCTC	1 cut(s)	1554
Sau3AI	GATC	9 cut(s)	189, 261, 544, 682, 1051, 1060, 1115, 1305, 2320
SchI	GAGTC	4 cut(s)	490, 1527, 2206, 2232
SduI	GDGCHC	5 cut(s)	471, 615, 791, 859, 1554
SfaNI	GCATC	4 cut(s)	1009, 1432, 1450, 1580
SfcI	CTRYAG	5 cut(s)	590, 1156, 1263, 1393, 1843
SfuI	TTCGAA	1 cut(s)	2281
SinI	GGWCC	3 cut(s)	184, 1142, 1277
SmiMI	CAYNNNNRTG	6 cut(s)	17, 1128, 1802, 2240, 2256, 2316
SmlI	CTYRAG	1 cut(s)	1214
SmoI	CTYRAG	1 cut(s)	1214
SpeI	ACTAGT	2 cut(s)	1970, 2065
Sse9I	AATT	7 cut(s)	667, 1135, 1863, 1901, 2028, 2174, 2286
SspMI	CTAG	8 cut(s)	63, 227, 702, 1971, 2001, 2066, 2123, 2133
SstI	GAGCTC	1 cut(s)	1554
StyI	CCWWGG	6 cut(s)	18, 132, 240, 256, 1198, 1222
TaaI	ACNGT	9 cut(s)	236, 850, 989, 1087, 1264, 1297, 1378, 1915, 1933
TaiI	ACGT	6 cut(s)	95, 476, 498, 582, 856, 2302
TaqI	TCGA	5 cut(s)	685, 735, 1485, 1497, 2281
TaqII	GACCGA	1 cut(s)	1530
TasI	AATT	7 cut(s)	667, 1135, 1863, 1901, 2028, 2174, 2286
TatI	WGTACW	1 cut(s)	2127
TauI	GCSGC	6 cut(s)	153, 632, 1254, 1614, 2147, 2354
TfiI	GAWTC	4 cut(s)	896, 1848, 2229, 2278
TscAI	CASTG	8 cut(s)	241, 784, 906, 1018, 1029, 1267, 1984, 2162
TseFI	GTSAC	2 cut(s)	2035, 2300
Tsp45I	GTSAC	2 cut(s)	2035, 2300
TspDTI	ATGAA	3 cut(s)	34, 284, 2228
TspRI	CASTG	8 cut(s)	241, 784, 906, 1018, 1029, 1267, 1984, 2162
Tth111I	GACNNNGTC	2 cut(s)	94, 1517
Van91I	CCANNNNNTGG	1 cut(s)	257
VneI	GTGCAC	1 cut(s)	855
VpaK11BI	GGWCC	3 cut(s)	184, 1142, 1277
XapI	RAATTY	2 cut(s)	1901, 2286
XceI	RCATGY	3 cut(s)	173, 1108, 2045
XcmI	CCANNNNNNNNNTGG	2 cut(s)	16, 1474
XmiI	GTMKAC	1 cut(s)	589
XspI	CTAG	8 cut(s)	63, 227, 702, 1971, 2001, 2066, 2123, 2133
ZraI	GACGTC	2 cut(s)	93, 2300

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.