Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0008307

Physical Location & Seq

Genomic Coordinates Forward (+)

65996502 .. 66001715

Gene Structure 5214 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G042370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2373 bp

ATGGCTTCCACCACCACCATGGCCTCCCTCTTCCTTTTCCTCCTCTTCATCCTTTTGCAAACAACTAGCAGCAAAGCGGCCGGCGAGTCTCCGACGTCATTCGCTTGCAACCCGCAAGACGCAAGCACCAAGGACTTGCCGTTTTGCCGCGTAAAGTTGCCGATACATGTGAGAGTGAGGGACCTGATCGGAAGGCTGACGTTGCAAGAGAAGGTGAAGCTGCTGGTGAACACTGCCAAGGCCGTGCCGCGCCTTGGGATCAAAGACTATGAATGGTGGTCGGAGGCACTTCATGGAGTCTCCAATGTGGGTCCCGGAACCAAGTTTGGCGGGGAGTTTCCGGGAGCCACCAGCTTCCCTCAGGTCATCACCACCGCTGCTTCCTTCAATGCTTCTCTGTGGGAAGCCATCGGACGGGTTGTGTCAGACGAAGCAAGAGCAATGTATAATGGAGGTATGGGCGGGCTCACGTATTGGAGTCCAAATGTGAACGTACTCAGAGACCCGAGATGGGGCCGTGGACAGGAAACTCCCGGCGAAGATCCGGTGGTGGTCGGAGCATATGCTGCCAGCTACGTCAGGGGTTTACAGGGCAATGACGGCAGCGGCCACCAGTTGAAGGTGGCTGCTTGCTGTAAACACTTCACAGCTTACGACCTTGATAACTGGCATGGAGTCGATCGATTTCACTTCAACGCTAGGGTTAGCAAGCAGGACATGGAGGACACATTCGACGTTCCTTTCAGGATGTGCGTCAAAGATGGAAAGGTGGCGAGTGTTATGTGCTCTTACAATCAGGTCAATGGTGTCCCAACCTGTGCAGATCCTAATCTCCTCAAGAAAACTGTACGTGCTCAATGGGGGCTTGATGGCTACATTGTCTCTGATTGCGATTCAGTGGGCGTTTTCTATGACAACCAACACTACACATCAACACCAGAAGAAGCAGCTGCTGCTGCCCTCAAAGCAGGTTTGGATTTAGACTGTGGCCCATTCCTGGGGTTGCACACTGAGGATGCAGTGAAGAAGGGTTTGTTGAGTGAGGTTGATCATATCGATCTTGCATTGGCTAATACACTCACAGTCCAAATGAGACTGGGCATGTTTGATGGATCTGCGCATCCATATGCTAATTTAGGTCCCAGGGATGTCTGCAGCCCAGCTCACCAGCAACTCGCTCTTGACGCTGCCACCCAAGGCATTGTTCTTCTCAAGAACCGTGGCCCTTCCCTGCCTTTATCCACCCGCCGCCACCGCAGTGTAGCTGTCATTGGTCCCAATTCTGATGTTACTGTTACTATGATCGGTAACTATGCCGGTGTTGCTTGTGGGTACACTACACCCCTACAAGGAATAGGAAAATACACCAGGACAGTTCACCAGCAGGGTTGCAGCGATGTAGCTTGCAAAGACGACACATTGTTTGGTGCAGCCATTGATGCAGCTCGTCAAGCCGATGCGACCATTTTAGTAATGGGCCTCGACCAGTCCATCGAGGCGGAGTTCAGAGACCGAGTTGGGCTACTTTTACCTGGACGGCAACAAGAGCTCATATCTAAAGTTGCTGCATCCGCTAAAGGCCCAACCATTCTGGTCTTGATGTCTGGTGGCCCAGTTGATGTTACTTTTGCCAAGAATGACCCACGTATCGCTGGCATTGTATGGGCCGGATACCCAGGACAAGCTGGTGGACAAGCCATTGCTGATATTGTGTTTGGAATATCAAATCCAGGAGGAAAGCTGCCTATGACATGGTACCCAGACGAGTATCTTAGAAATTTGGAAATGACAGACATGGGGATGAGGTCAAGGCCATCAAAAGGGTACCCTGGAAGAACCTACAGATTCTACAAAGGGCCAATAGTATACCCATTTGGACATGGATTGAGTTACACTAAATTTGTGCAAACCGTAGCGAGCGCCCCCACTGTAGTTGCAATTGTTCTTGATGGTCACCATACTGAGACTAGTACTAGTGCCGGTAACGGTACTTCATTCGCAACTGCCGGGAAGGCAATTCGCGTGACACATGCGAAATGCAGCAAGCTTTCGCTCGTTGTCGATGTGGATGTGAGAAATGTGGGCAACAGAGACGGGTCTCACACTTTGCTAGTGTACTCGAGCCCTCCAGCTGCTGCCGGACACTGGGTGCCACACAAACAATTGGTTGCCTTTGAGAAAGTACATGTCCTGGCAAGGACTCAACAGCGAGTCCGATTCAACATTCATGTGTGCAAGCACCTTAGTGTGGTGGACAGGTCTGGGATTCGAAGAATTCCGATGGGACGGCACGACCTCCACATTGGTGATCATGTCGTCCACTCTTTCTCCGTCCAAGCCTCTTCACTGGGGTTGGGAATCATCAAATCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

790

Amino Acids

85.09

Weight (kDa)

8.65

Isoelectric Point (pI)

30.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	113 - 328	4.2e-37	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	401 - 631	1.2e-50	Glycosyl hydrolase family 3 C-terminal domain
Fn3-like	PF14310	702 - 771	3.3e-10	Fibronectin type III-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008307)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02640
fragaria_vesca	FvH4_5g39430 FvH4_5g39430 FvH4_5g39430 FvH4_5g39430
malus_domestica	MD03G1296500.v1.1 MD11G1315900.v1.1
prunus_persica	Prupe.8G270800_v2.0.a1 Prupe.8G270800_v2.0.a1
pyrus_communis	pycom03g23490 pycom11g27580
rosa_chinensis	RchiOBHm_Chr7g0241741
rosa_laevigata	RLG00000000548
rosa_multiflora	Rmu_co8482979.1_g000001 Rmu_sc0010736.1_g000003 Rmu_sc0029163.1_g000002
rosa_roxburghii	Rroxscaffold_3G00218710
rosa_rugosa	Rorug07G0346900
rosa_samantha	Rh7AG501300 Rh7BG472600 Rh7CG517600 Rh7DG486300
rosa_wichuraiana	Rw7G042370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	98
Acc16I	TGCGCA	1 cut(s)	1119
Acc36I	ACCTGC	1 cut(s)	959
Acc65I	GGTACC	2 cut(s)	1755, 1824
AccB1I	GGYRCC	3 cut(s)	1755, 1824, 2149
AccI	GTMKAC	1 cut(s)	1866
AccII	CGCG	3 cut(s)	150, 250, 2022
AclWI	GGATC	4 cut(s)	266, 536, 818, 1120
AcoI	YGGCCR	2 cut(s)	78, 607
AcsI	RAATTY	3 cut(s)	1777, 1898, 2274
AcyI	GRCGYC	1 cut(s)	95
AdeI	CACNNNGTG	1 cut(s)	2149
AfaI	GTAC	9 cut(s)	495, 849, 1334, 1757, 1826, 1972, 1990, 2117, 2184
AflIII	ACRYGT	2 cut(s)	166, 2185
AgsI	TTSAA	4 cut(s)	388, 619, 694, 2221
AhlI	ACTAGT	2 cut(s)	1967, 1973
AjnI	CCWGG	8 cut(s)	996, 1142, 1367, 1531, 1675, 1729, 1828, 2190
AjuI	GAANNNNNNNTTGG	2 cut(s)	1188, 1220
AleI	CACNNNNGTG	3 cut(s)	1257, 2244, 2304
Alw21I	GWGCWC	3 cut(s)	788, 856, 1551
Alw26I	GTCTC	9 cut(s)	93, 304, 495, 886, 1087, 1503, 1958, 2085, 2103
AlwI	GGATC	4 cut(s)	266, 536, 818, 1120
AlwNI	CAGNNNCTG	2 cut(s)	953, 2135
Ama87I	CYCGRG	2 cut(s)	505, 2119
ApoI	RAATTY	3 cut(s)	1777, 1898, 2274
ArsI	GACNNNNNNTTYG	2 cut(s)	1406, 1438
Asp718I	GGTACC	2 cut(s)	1755, 1824
AspLEI	GCGC	3 cut(s)	252, 1120, 1922
AsuC2I	CCSGG	4 cut(s)	315, 342, 534, 2008
AsuHPI	GGTGA	7 cut(s)	226, 238, 361, 1157, 1370, 1946, 2318
AsuII	TTCGAA	1 cut(s)	2269
AvaI	CYCGRG	2 cut(s)	505, 2119
AvaII	GGWCC	4 cut(s)	181, 311, 1139, 1274
AxyI	CCTNAGG	1 cut(s)	360
BaeI	ACNNNNGTAYC	2 cut(s)	1630, 1663
BanI	GGYRCC	3 cut(s)	1755, 1824, 2149
BanII	GRGCYC	3 cut(s)	468, 1551, 2126
BarI	GAAGNNNNNNTAC	2 cut(s)	1975, 2007
Bbv12I	GWGCWC	3 cut(s)	788, 856, 1551
BccI	CCATC	9 cut(s)	416, 504, 755, 863, 1103, 1499, 1822, 1943, 2275
BceAI	ACGGC	6 cut(s)	124, 227, 501, 616, 1553, 2303
BciT130I	CCWGG	8 cut(s)	998, 1144, 1369, 1533, 1677, 1731, 1830, 2192
BciVI	GTATCC	1 cut(s)	1664
BclI	TGATCA	2 cut(s)	1048, 2308
BcnI	CCSGG	4 cut(s)	315, 342, 534, 2008
BcoDI	GTCTC	9 cut(s)	93, 304, 495, 886, 1087, 1503, 1958, 2085, 2103
BcuI	ACTAGT	2 cut(s)	1967, 1973
BfaI	CTAG	5 cut(s)	66, 699, 1968, 1974, 2111
BfmI	CTRYAG	3 cut(s)	1153, 1840, 1929
BfoI	RGCGCY	1 cut(s)	1923
BfuAI	ACCTGC	1 cut(s)	959
BfuI	GTATCC	1 cut(s)	1664
BglI	GCCNNNNNGGC	1 cut(s)	2012
BmcAI	AGTACT	1 cut(s)	1972
Bme18I	GGWCC	4 cut(s)	181, 311, 1139, 1274
BmeT110I	CYCGRG	2 cut(s)	505, 2119
BmrI	ACTGGG	4 cut(s)	1106, 1607, 2155, 2357
BmsI	GCATC	5 cut(s)	1006, 1129, 1429, 1447, 1577
BmuI	ACTGGG	4 cut(s)	1106, 1607, 2155, 2357
BpmI	CTGGAG	1 cut(s)	2112
Bpu14I	TTCGAA	1 cut(s)	2269
BpuEI	CTTGAG	2 cut(s)	821, 1196
BpuMI	CCSGG	4 cut(s)	315, 342, 534, 2008
Bsa29I	ATCGAT	2 cut(s)	682, 1056
BsaAI	YACGTR	3 cut(s)	471, 851, 1646
BsaBI	GATNNNNATC	1 cut(s)	828
BsaHI	GRCGYC	1 cut(s)	95
BsaI	GGTCTC	3 cut(s)	495, 1503, 2103
BsaWI	WCCGGW	1 cut(s)	544
BsaXI	ACNNNNNCTCC	2 cut(s)	2312, 2342
Bse118I	RCCGGY	3 cut(s)	80, 1316, 1979
Bse1I	ACTGG	7 cut(s)	613, 671, 1101, 1486, 1613, 2150, 2352
Bse21I	CCTNAGG	1 cut(s)	360
Bse3DI	GCAATG	3 cut(s)	447, 601, 1698
Bse8I	GATNNNNATC	1 cut(s)	828
BseBI	CCWGG	8 cut(s)	998, 1144, 1369, 1533, 1677, 1731, 1830, 2192
BseCI	ATCGAT	2 cut(s)	682, 1056
BseGI	GGATG	8 cut(s)	48, 753, 1021, 1120, 1153, 1568, 1806, 2074
BseJI	GATNNNNATC	1 cut(s)	828
BseMI	GCAATG	3 cut(s)	447, 601, 1698
BseMII	CTCAG	4 cut(s)	374, 511, 1002, 1953
BseNI	ACTGG	7 cut(s)	613, 671, 1101, 1486, 1613, 2150, 2352
BseRI	GAGGAG	2 cut(s)	32, 824
BseX3I	CGGCCG	1 cut(s)	78
BseYI	CCCAGC	1 cut(s)	1159
BsgI	GTGCAG	2 cut(s)	840, 1449
Bsh1236I	CGCG	3 cut(s)	150, 250, 2022
Bsh1285I	CGRYCG	2 cut(s)	81, 682
BshNI	GGYRCC	3 cut(s)	1755, 1824, 2149
BshVI	ATCGAT	2 cut(s)	682, 1056
BsiEI	CGRYCG	2 cut(s)	81, 682
BsiHKAI	GWGCWC	3 cut(s)	788, 856, 1551
BsiHKCI	CYCGRG	2 cut(s)	505, 2119
BslFI	GGGAC	6 cut(s)	194, 297, 794, 1125, 1260, 2298
BsmAI	GTCTC	9 cut(s)	93, 304, 495, 886, 1087, 1503, 1958, 2085, 2103
BsmBI	CGTCTC	1 cut(s)	2085
BsmFI	GGGAC	6 cut(s)	194, 297, 794, 1125, 1260, 2298
Bso31I	GGTCTC	3 cut(s)	495, 1503, 2103
BsoBI	CYCGRG	2 cut(s)	505, 2119
Bsp119I	TTCGAA	1 cut(s)	2269
Bsp1286I	GDGCHC	5 cut(s)	468, 788, 856, 1551, 2126
Bsp19I	CCATGG	1 cut(s)	18
BspCNI	CTCAG	4 cut(s)	373, 510, 1003, 1954
BspDI	ATCGAT	2 cut(s)	682, 1056
BspFNI	CGCG	3 cut(s)	150, 250, 2022
BspMAI	CTGCAG	1 cut(s)	1157
BspMI	ACCTGC	1 cut(s)	959
BspPI	GGATC	4 cut(s)	266, 536, 818, 1120
BspT104I	TTCGAA	1 cut(s)	2269
BspT107I	GGYRCC	3 cut(s)	1755, 1824, 2149
BspTNI	GGTCTC	3 cut(s)	495, 1503, 2103
BsrDI	GCAATG	3 cut(s)	447, 601, 1698
BsrFI	RCCGGY	3 cut(s)	80, 1316, 1979
BsrI	ACTGG	7 cut(s)	613, 671, 1101, 1486, 1613, 2150, 2352
BssAI	RCCGGY	3 cut(s)	80, 1316, 1979
BssNAI	GTATAC	1 cut(s)	1867
BssNI	GRCGYC	1 cut(s)	95
BssT1I	CCWWGG	5 cut(s)	18, 129, 237, 253, 1195
Bst1107I	GTATAC	1 cut(s)	1867
Bst2UI	CCWGG	8 cut(s)	998, 1144, 1369, 1533, 1677, 1731, 1830, 2192
Bst4CI	ACNGT	9 cut(s)	847, 986, 1084, 1220, 1294, 1375, 1912, 1930, 1988
Bst6I	CTCTTC	3 cut(s)	35, 50, 2347
BstACI	GRCGYC	1 cut(s)	95
BstAPI	GCANNNNNTGC	2 cut(s)	566, 953
BstBAI	YACGTR	3 cut(s)	471, 851, 1646
BstBI	TTCGAA	1 cut(s)	2269
BstDEI	CTNAG	6 cut(s)	360, 497, 1011, 1772, 1962, 2243
BstDSI	CCRYGG	3 cut(s)	18, 517, 1219
BstEII	GGTNACC	1 cut(s)	1952
BstF5I	GGATG	8 cut(s)	48, 753, 1021, 1120, 1153, 1568, 1806, 2074
BstFNI	CGCG	3 cut(s)	150, 250, 2022
BstH2I	RGCGCY	1 cut(s)	1923
BstHHI	GCGC	3 cut(s)	252, 1120, 1922
BstMAI	GTCTC	9 cut(s)	93, 304, 495, 886, 1087, 1503, 1958, 2085, 2103
BstMCI	CGRYCG	2 cut(s)	81, 682
BstNI	CCWGG	8 cut(s)	998, 1144, 1369, 1533, 1677, 1731, 1830, 2192
BstNSI	RCATGY	4 cut(s)	170, 1105, 2033, 2189
BstPI	GGTNACC	1 cut(s)	1952
BstSFI	CTRYAG	3 cut(s)	1153, 1840, 1929
BstUI	CGCG	3 cut(s)	150, 250, 2022
BstX2I	RGATCY	3 cut(s)	541, 823, 1112
BstXI	CCANNNNNNTGG	1 cut(s)	19
BstYI	RGATCY	3 cut(s)	541, 823, 1112
BstZ17I	GTATAC	1 cut(s)	1867
BstZI	CGGCCG	1 cut(s)	78
Bsu15I	ATCGAT	2 cut(s)	682, 1056
Bsu36I	CCTNAGG	1 cut(s)	360
BsuI	GTATCC	1 cut(s)	1664
BsuTUI	ATCGAT	2 cut(s)	682, 1056
BtgI	CCRYGG	3 cut(s)	18, 517, 1219
BtgZI	GCGATG	1 cut(s)	1410
BtsCI	GGATG	8 cut(s)	48, 753, 1021, 1120, 1153, 1568, 1806, 2074
BtsI	GCAGTG	3 cut(s)	231, 1026, 1264
BtsIMutI	CAGTG	8 cut(s)	231, 903, 1008, 1026, 1264, 1926, 2143, 2345
BveI	ACCTGC	1 cut(s)	959
CaiI	CAGNNNCTG	2 cut(s)	953, 2135
CfoI	GCGC	3 cut(s)	252, 1120, 1922
Cfr10I	RCCGGY	3 cut(s)	80, 1316, 1979
ClaI	ATCGAT	2 cut(s)	682, 1056
CseI	GACGC	3 cut(s)	128, 742, 1193
Csp6I	GTAC	9 cut(s)	494, 848, 1333, 1756, 1825, 1971, 1989, 2116, 2183
CspCI	CAANNNNNGTGG	4 cut(s)	1201, 1236, 1915, 1950
CviQI	GTAC	9 cut(s)	494, 848, 1333, 1756, 1825, 1971, 1989, 2116, 2183
DdeI	CTNAG	6 cut(s)	360, 497, 1011, 1772, 1962, 2243
DraIII	CACNNNGTG	1 cut(s)	2149
EaeI	YGGCCR	2 cut(s)	78, 607
EagI	CGGCCG	1 cut(s)	78
Eam1104I	CTCTTC	3 cut(s)	35, 50, 2347
EarI	CTCTTC	3 cut(s)	35, 50, 2347
EciI	GGCGGA	1 cut(s)	1514
Ecl136II	GAGCTC	1 cut(s)	1549
EclXI	CGGCCG	1 cut(s)	78
Eco130I	CCWWGG	5 cut(s)	18, 129, 237, 253, 1195
Eco24I	GRGCYC	3 cut(s)	468, 1551, 2126
Eco31I	GGTCTC	3 cut(s)	495, 1503, 2103
Eco47I	GGWCC	4 cut(s)	181, 311, 1139, 1274
Eco52I	CGGCCG	1 cut(s)	78
Eco53kI	GAGCTC	1 cut(s)	1549
Eco81I	CCTNAGG	1 cut(s)	360
Eco88I	CYCGRG	2 cut(s)	505, 2119
Eco91I	GGTNACC	1 cut(s)	1952
EcoICRI	GAGCTC	1 cut(s)	1549
EcoO109I	RGGNCCY	3 cut(s)	181, 311, 1139
EcoO65I	GGTNACC	1 cut(s)	1952
EcoRI	GAATTC	1 cut(s)	2274
EcoRII	CCWGG	8 cut(s)	996, 1142, 1367, 1531, 1675, 1729, 1828, 2190
EcoT14I	CCWWGG	5 cut(s)	18, 129, 237, 253, 1195
EcoT38I	GRGCYC	3 cut(s)	468, 1551, 2126
ErhI	CCWWGG	5 cut(s)	18, 129, 237, 253, 1195
Esp3I	CGTCTC	1 cut(s)	2085
FaqI	GGGAC	6 cut(s)	194, 297, 794, 1125, 1260, 2298
FauI	CCCGC	4 cut(s)	120, 323, 455, 1253
FauNDI	CATATG	2 cut(s)	562, 1126
FbaI	TGATCA	2 cut(s)	1048, 2308
FblI	GTMKAC	1 cut(s)	1866
FokI	GGATG	8 cut(s)	35, 760, 1028, 1107, 1160, 1555, 1813, 2081
FriOI	GRGCYC	3 cut(s)	468, 1551, 2126
FspBI	CTAG	5 cut(s)	66, 699, 1968, 1974, 2111
FspI	TGCGCA	1 cut(s)	1119
GlaI	GCGC	3 cut(s)	251, 1119, 1921
GsaI	CCCAGC	1 cut(s)	1163
GsuI	CTGGAG	1 cut(s)	2112
HaeII	RGCGCY	1 cut(s)	1923
HgaI	GACGC	3 cut(s)	128, 742, 1193
HhaI	GCGC	3 cut(s)	252, 1120, 1922
Hin1I	GRCGYC	1 cut(s)	95
Hin6I	GCGC	3 cut(s)	250, 1118, 1920
HinP1I	GCGC	3 cut(s)	250, 1118, 1920
HindIII	AAGCTT	1 cut(s)	2045
HphI	GGTGA	7 cut(s)	226, 238, 361, 1157, 1370, 1946, 2318
Hpy188III	TCNNGA	7 cut(s)	745, 838, 1181, 1213, 1597, 1946, 2370
Hpy99I	CGWCG	2 cut(s)	97, 737
HpyAV	CCTTC	7 cut(s)	186, 205, 394, 613, 1021, 1236, 2005
HpyCH4III	ACNGT	9 cut(s)	847, 986, 1084, 1220, 1294, 1375, 1912, 1930, 1988
HpyCH4IV	ACGT	8 cut(s)	95, 200, 470, 492, 576, 735, 850, 1645
HpyF3I	CTNAG	6 cut(s)	360, 497, 1011, 1772, 1962, 2243
HpySE526I	ACGT	8 cut(s)	95, 200, 470, 492, 576, 735, 850, 1645
Hsp92I	GRCGYC	1 cut(s)	95
HspAI	GCGC	3 cut(s)	250, 1118, 1920
KflI	GGGWCCC	1 cut(s)	311
KpnI	GGTACC	2 cut(s)	1759, 1828
KroI	GCCGGC	1 cut(s)	80
KroNI	GCCGGC	1 cut(s)	82
Ksp22I	TGATCA	2 cut(s)	1048, 2308
LmnI	GCTCC	2 cut(s)	344, 557
LweI	GCATC	5 cut(s)	1006, 1129, 1429, 1447, 1577
MaeI	CTAG	5 cut(s)	66, 699, 1968, 1974, 2111
MaeII	ACGT	8 cut(s)	95, 200, 470, 492, 576, 735, 850, 1645
MaeIII	GTNAC	8 cut(s)	1288, 1294, 1307, 1621, 1889, 1952, 1982, 2023
MboII	GAAGA	9 cut(s)	22, 37, 551, 953, 1036, 1199, 1845, 2283, 2334
MfeI	CAATTG	2 cut(s)	1938, 2162
MflI	RGATCY	3 cut(s)	541, 823, 1112
MhlI	GDGCHC	5 cut(s)	468, 788, 856, 1551, 2126
MluCI	AATT	8 cut(s)	1132, 1279, 1777, 1898, 1938, 2016, 2162, 2274
MlyI	GAGTC	6 cut(s)	95, 306, 487, 684, 2194, 2220
MmeI	TCCRAC	3 cut(s)	116, 261, 535
MroNI	GCCGGC	1 cut(s)	80
MslI	CAYNNNNRTG	7 cut(s)	17, 1125, 1257, 1799, 2228, 2244, 2304
MspA1I	CMGCKG	4 cut(s)	377, 606, 950, 2132
MunI	CAATTG	2 cut(s)	1938, 2162
MvaI	CCWGG	8 cut(s)	998, 1144, 1369, 1533, 1677, 1731, 1830, 2192
MvnI	CGCG	3 cut(s)	150, 250, 2022
NaeI	GCCGGC	1 cut(s)	82
NciI	CCSGG	4 cut(s)	315, 342, 534, 2008
NcoI	CCATGG	1 cut(s)	18
NdeI	CATATG	2 cut(s)	562, 1126
NgoMIV	GCCGGC	1 cut(s)	80
NmuCI	GTSAC	2 cut(s)	1952, 2023
NsbI	TGCGCA	1 cut(s)	1119
NspI	RCATGY	4 cut(s)	170, 1105, 2033, 2189
NspV	TTCGAA	1 cut(s)	2269
OliI	CACNNNNGTG	3 cut(s)	1257, 2244, 2304
PaeR7I	CTCGAG	1 cut(s)	2119
PasI	CCCWGGG	1 cut(s)	1143
PciI	ACATGT	2 cut(s)	166, 2185
PdiI	GCCGGC	1 cut(s)	82
PfeI	GAWTC	5 cut(s)	893, 1845, 2217, 2266, 2358
PfoI	TCCNGGA	3 cut(s)	313, 340, 1729
Ple19I	CGATCG	1 cut(s)	682
PleI	GAGTC	6 cut(s)	94, 305, 486, 683, 2194, 2219
PpsI	GAGTC	6 cut(s)	94, 305, 486, 683, 2194, 2219
Ppu21I	YACGTR	3 cut(s)	471, 851, 1646
PpuMI	RGGWCCY	3 cut(s)	181, 311, 1139
PscI	ACATGT	2 cut(s)	166, 2185
Psp124BI	GAGCTC	1 cut(s)	1551
Psp5II	RGGWCCY	3 cut(s)	181, 311, 1139
Psp6I	CCWGG	8 cut(s)	996, 1142, 1367, 1531, 1675, 1729, 1828, 2190
PspEI	GGTNACC	1 cut(s)	1952
PspFI	CCCAGC	1 cut(s)	1159
PspGI	CCWGG	8 cut(s)	996, 1142, 1367, 1531, 1675, 1729, 1828, 2190
PspPPI	RGGWCCY	3 cut(s)	181, 311, 1139
PspXI	VCTCGAGB	1 cut(s)	2119
PstI	CTGCAG	1 cut(s)	1157
PstNI	CAGNNNCTG	2 cut(s)	953, 2135
PsuI	RGATCY	3 cut(s)	541, 823, 1112
PvuI	CGATCG	1 cut(s)	682
PvuII	CAGCTG	2 cut(s)	950, 2132
RsaI	GTAC	9 cut(s)	495, 849, 1334, 1757, 1826, 1972, 1990, 2117, 2184
RsaNI	GTAC	9 cut(s)	494, 848, 1333, 1756, 1825, 1971, 1989, 2116, 2183
RseI	CAYNNNNRTG	7 cut(s)	17, 1125, 1257, 1799, 2228, 2244, 2304
SacI	GAGCTC	1 cut(s)	1551
ScaI	AGTACT	1 cut(s)	1972
SchI	GAGTC	6 cut(s)	95, 306, 487, 684, 2194, 2220
SduI	GDGCHC	5 cut(s)	468, 788, 856, 1551, 2126
SfaNI	GCATC	5 cut(s)	1006, 1129, 1429, 1447, 1577
SfcI	CTRYAG	3 cut(s)	1153, 1840, 1929
Sfr274I	CTCGAG	1 cut(s)	2119
SfuI	TTCGAA	1 cut(s)	2269
SinI	GGWCC	4 cut(s)	181, 311, 1139, 1274
SlaI	CTCGAG	1 cut(s)	2119
SmiMI	CAYNNNNRTG	7 cut(s)	17, 1125, 1257, 1799, 2228, 2244, 2304
SmlI	CTYRAG	3 cut(s)	836, 1211, 2119
SmoI	CTYRAG	3 cut(s)	836, 1211, 2119
SpeI	ACTAGT	2 cut(s)	1967, 1973
Sse9I	AATT	8 cut(s)	1132, 1279, 1777, 1898, 1938, 2016, 2162, 2274
SspMI	CTAG	5 cut(s)	66, 699, 1968, 1974, 2111
SstI	GAGCTC	1 cut(s)	1551
StyI	CCWWGG	5 cut(s)	18, 129, 237, 253, 1195
TaaI	ACNGT	9 cut(s)	847, 986, 1084, 1220, 1294, 1375, 1912, 1930, 1988
TaiI	ACGT	8 cut(s)	98, 203, 473, 495, 579, 738, 853, 1648
TaqI	TCGA	9 cut(s)	678, 682, 732, 1056, 1482, 1494, 2061, 2120, 2269
TaqII	GACCGA	1 cut(s)	1527
TasI	AATT	8 cut(s)	1132, 1279, 1777, 1898, 1938, 2016, 2162, 2274
TatI	WGTACW	3 cut(s)	1970, 2115, 2182
TauI	GCSGC	5 cut(s)	80, 150, 250, 609, 1251
TfiI	GAWTC	5 cut(s)	893, 1845, 2217, 2266, 2358
TscAI	CASTG	8 cut(s)	238, 903, 1015, 1026, 1264, 1933, 2150, 2352
TseFI	GTSAC	2 cut(s)	1952, 2023
Tsp45I	GTSAC	2 cut(s)	1952, 2023
TspDTI	ATGAA	5 cut(s)	37, 281, 285, 1983, 2216
TspGWI	ACGGA	1 cut(s)	2320
TspRI	CASTG	8 cut(s)	238, 903, 1015, 1026, 1264, 1933, 2150, 2352
VpaK11BI	GGWCC	4 cut(s)	181, 311, 1139, 1274
XapI	RAATTY	3 cut(s)	1777, 1898, 2274
XceI	RCATGY	4 cut(s)	170, 1105, 2033, 2189
XcmI	CCANNNNNNNNNTGG	2 cut(s)	16, 1471
XhoI	CTCGAG	1 cut(s)	2119
XmiI	GTMKAC	1 cut(s)	1866
XspI	CTAG	5 cut(s)	66, 699, 1968, 1974, 2111
ZraI	GACGTC	1 cut(s)	96
ZrmI	AGTACT	1 cut(s)	1972

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.