Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0008307

Physical Location & Seq

Genomic Coordinates Forward (+)

42780270 .. 42786684

Gene Structure 6415 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1315900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2367 bp

ATGGCTACTTCCATGCCCGCCATTATTAAGCTCTGCAGCCTTCTCCTCCTTTTAGTTTTAGTTTGGGGTGTTTTCGAACAACCCAAAACTTGCGAAGGCGGTGAGTCCTTTGCTTGCAACCCAAAAGATGCGGCGACCAAGGACTTGCCGTTTTGCCGTGCTACATTGCCCGTGCAAGACAGAGTGAGGGACCTTCTCGGAAGGTTGACATTGCAAGAGAAGGTTAAGCTGCTGGTGAACACTGCTCTGCCTGTCCCACGCCTTGGCATCAAGGGCTACGAGTGGTGGTCCGAGGCACTTCACGGAGTTTCCAATGTGGGCCCCGGGACTAAGTTTGGTGGGGTCTTCCCTGGTGCCACTAGCTTCCCTCAAGTCATCACAACCGCTGCTTCCTTCAATGCTTCTTTGTGGGAAGCCATCGGACGGGTCGTGTCAGACGAAGCAAGAGCAATGTACAACGGGGGTGTGGCTGGCCTTACGTATTGGAGCCCAAATGTGAACATACTAAGAGACCCACGGTGGGGCCGCGCACAAGAGACTCCCGGTGAAGACCCAACCGTAGTGGGTAGATATGCTGCCAGCTACGTTAGGGGGTTGCAGGGAGATGACAGTAACCGGTTGAAGGTGGCGGCCTGTTGTAAGCACTTCACCGCTTATGACCTCGACAACTGGAACGGAGTTGATCGTTTTCACTTTAATGCTAGGGTGAGCAAGCAGGACATGGAGGACACGTTCGATGTGCCTTTCAGGATGTGCGTCAAGGAAGGAAAGGTGGCCAGTGTCATGTGTTCTTACAATCAGGTCAATGGTGTCCCAACCTGTGCCGACCCTAATCTCCTAAAAAAAACTGTACGCGGTGCATGGCGCCTCGACGGCTACATTGTGTCTGATTGCGACTCAGTGGGGGTGTTCTATAATAGCCAACACTACACCTCAACTCCGGAAGAAGCCGCTGCCGCTGCCATTAAAGCAGGTTTGGATTTAGACTGTGGGCCATTCTTGGCCGTGCACTCGGAAGAAGCAGTGAACAAGGGGTTGTTGAAGGAGGTTGACATCAACAATGCATTGGTGAATACTGTGACAGTCCAAATGAGACTCGGGATGTTTGATGGAGACCGCGGGTCCTATGCCAGATTGGGCCCCAAAGACGTATGCAGCCCACCTCATCAGGATCTCGCCCTTGAGGCCGCCGCGCAAGGCATCGTCCTCCTCAAGAATCACGGGCCCTCGCTCCCTTTGTCCACCCGTCGTCACCGTACTGTTGCTGTCATCGGCCCCAATTCTGATGTTACTCTCACTATGATTGGCAACTATGCCGGTGTGGCATGTGGATACACTACGCCACTACAAGGGCTTGGGAATTTTGCAAGGACAATTCACCAGGCGGGTTGTGCGGACGTCGCTTGTGCCAACGACACGCTATTCCAAGCAGCCATCGACGCATCCCGGGAAGCGGATGCGAGTGTTCTGGTGATGGGGCTTGATCAGTCCATTGAGGCTGAATTCAGAGACCGAGCTGGGCTGCTTTTACCTGGACGACAGCAGGACCTCATATCAAAGGTTGCTGCAGCATCCAAGGGCCCAACTATTCTGGTATTGATGTCTGGTGGGTCAATTGACGTGTCTTTTGCTAACAATGACCCGCGCATCGCTGGAATTGTATGGGCCGGCTACCCGGGCCAGGCTGGTGGAGCAGCCATTGCTCGTGTTTTGTTTGGAGTCACAAACCCAGGAGGAAAGCTTCCTATGACGTGGTACCCAGAGGAGTATCTTAACAATTTGGCAATGACATCGATGGACATGAGGTCAAGCCCATCAAAAGGGTACCCTGGAAGAACCTATAGATTCTACAGAGGGCCAGTGTTATACCCGTTTGGGCACGGATTGAGCTACACCAAATTTGTTCTTAGCATAGCAGCCGGTACCGCACCCACTGTGGTTGGAATCCAGCTTGATGGCCGGCATCGCGCCTCTCAAAACACAACCGCAACTATTGATTCGGACAATGTCAAGGCGATCCGAGTAACGCATGCAAAATGCAACGGGGTTTCAGTGGGTTTTGACGTTGACGTGAGAAATGTGGGTAACAGAGACGGGTCTCACACTTTGCTAGTGTTCTCTACCCCTCCAGCACAGGACTGGGCACCTCACAAGCAGCTGGTTACCTTTGAGAAAGTGTATGTTCCGGCCAGGGCTCAACGGCGGTTGCGAATCAACATTCATGTGTGCAAGTCCCTTAGCGTGGTGGACAGGTTTGGAATTCGAAGAATTCCAATGGGACAACACAATCTTCACATCGGTGACCTCATGCACTCTGTCTCCCTCAAACCCGCCAGTTCATTAGGGTTGGGGGTCATCAAATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

789

Amino Acids

84.94

Weight (kDa)

8.68

Isoelectric Point (pI)

33.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	115 - 356	4.7e-35	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	401 - 631	2.2e-49	Glycosyl hydrolase family 3 C-terminal domain
Fn3-like	PF14310	706 - 768	4.2e-07	Fibronectin type III-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008307)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02640
fragaria_vesca	FvH4_5g39430 FvH4_5g39430 FvH4_5g39430 FvH4_5g39430
malus_domestica	MD03G1296500.v1.1 MD11G1315900.v1.1
prunus_persica	Prupe.8G270800_v2.0.a1 Prupe.8G270800_v2.0.a1
pyrus_communis	pycom03g23490 pycom11g27580
rosa_chinensis	RchiOBHm_Chr7g0241741
rosa_laevigata	RLG00000000548
rosa_multiflora	Rmu_co8482979.1_g000001 Rmu_sc0010736.1_g000003 Rmu_sc0029163.1_g000002
rosa_roxburghii	Rroxscaffold_3G00218710
rosa_rugosa	Rorug07G0346900
rosa_samantha	Rh7AG501300 Rh7BG472600 Rh7CG517600 Rh7DG486300
rosa_wichuraiana	Rw7G042370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1401
Acc36I	ACCTGC	1 cut(s)	962
Acc65I	GGTACC	3 cut(s)	1755, 1824, 1922
AccB1I	GGYRCC	6 cut(s)	353, 864, 1755, 1824, 1922, 2143
AccB7I	CCANNNNNTGG	1 cut(s)	263
AccII	CGCG	6 cut(s)	528, 855, 1119, 1193, 1645, 1968
AccIII	TCCGGA	1 cut(s)	940
AclWI	GGATC	2 cut(s)	1179, 2011
AcoI	YGGCCR	4 cut(s)	774, 1002, 1957, 2187
AcsI	RAATTY	5 cut(s)	1360, 1502, 1898, 2259, 2268
AcyI	GRCGYC	2 cut(s)	865, 1398
AfaI	GTAC	6 cut(s)	455, 852, 1258, 1757, 1826, 1924
AflIII	ACRYGT	2 cut(s)	729, 1620
AgeI	ACCGGT	1 cut(s)	615
AgsI	TTSAA	3 cut(s)	397, 622, 1042
AhdI	GACNNNNNGTC	2 cut(s)	1120, 1804
AjiI	CACGTC	3 cut(s)	1621, 1752, 2071
AjnI	CCWGG	7 cut(s)	349, 1380, 1531, 1680, 1729, 1828, 2189
AleI	CACNNNNGTG	1 cut(s)	2298
AloI	GAACNNNNNNTCC	2 cut(s)	716, 748
AluBI	AGCT	9 cut(s)	31, 229, 363, 582, 1517, 1741, 1890, 1951, 2158
AluI	AGCT	9 cut(s)	31, 229, 363, 582, 1517, 1741, 1890, 1951, 2158
Alw21I	GWGCWC	1 cut(s)	1011
Alw26I	GTCTC	8 cut(s)	504, 530, 1087, 1107, 1503, 2085, 2103, 2323
Alw44I	GTGCAC	1 cut(s)	1007
AlwI	GGATC	2 cut(s)	1179, 2011
Ama87I	CYCGRG	4 cut(s)	323, 1097, 1446, 1675
Aor13HI	TCCGGA	1 cut(s)	940
ApaI	GGGCCC	4 cut(s)	323, 1142, 1227, 1583
ApaLI	GTGCAC	1 cut(s)	1007
ApoI	RAATTY	5 cut(s)	1360, 1502, 1898, 2259, 2268
ArsI	GACNNNNNNTTYG	2 cut(s)	1610, 1642
AsiGI	ACCGGT	1 cut(s)	615
Asp718I	GGTACC	3 cut(s)	1755, 1824, 1922
AspLEI	GCGC	5 cut(s)	530, 867, 1195, 1647, 1970
AsuC2I	CCSGG	7 cut(s)	324, 325, 543, 1447, 1448, 1676, 1677
AsuII	TTCGAA	2 cut(s)	75, 2263
AvaI	CYCGRG	4 cut(s)	323, 1097, 1446, 1675
AvaII	GGWCC	4 cut(s)	190, 288, 1122, 1546
BaeGI	GKGCMC	7 cut(s)	323, 1011, 1142, 1227, 1583, 1881, 2146
BalI	TGGCCA	1 cut(s)	776
BanI	GGYRCC	6 cut(s)	353, 864, 1755, 1824, 1922, 2143
BanII	GRGCYC	6 cut(s)	323, 491, 1142, 1227, 1583, 2197
BauI	CACGAG	1 cut(s)	1704
BbsI	GAAGAC	2 cut(s)	337, 555
Bbv12I	GWGCWC	1 cut(s)	1011
BccI	CCATC	7 cut(s)	425, 1103, 1442, 1468, 1789, 1822, 1949
BceAI	ACGGC	5 cut(s)	133, 141, 889, 989, 2216
BcgI	CGANNNNNNTGC	2 cut(s)	1773, 1807
BciT130I	CCWGG	7 cut(s)	351, 1382, 1533, 1682, 1731, 1830, 2191
BciVI	GTATCC	1 cut(s)	1325
BclI	TGATCA	1 cut(s)	1483
BcnI	CCSGG	7 cut(s)	324, 325, 543, 1447, 1448, 1676, 1677
BcoDI	GTCTC	8 cut(s)	504, 530, 1087, 1107, 1503, 2085, 2103, 2323
BfaI	CTAG	3 cut(s)	360, 702, 2111
BfmI	CTRYAG	4 cut(s)	34, 1566, 1840, 1849
BfoI	RGCGCY	1 cut(s)	868
BfuAI	ACCTGC	1 cut(s)	962
BfuI	GTATCC	1 cut(s)	1325
BglI	GCCNNNNNGGC	3 cut(s)	873, 1184, 1322
Bme18I	GGWCC	4 cut(s)	190, 288, 1122, 1546
BmeRI	GACNNNNNGTC	2 cut(s)	1120, 1804
BmeT110I	CYCGRG	4 cut(s)	323, 1097, 1446, 1675
BmgBI	CACGTC	3 cut(s)	1621, 1752, 2071
BmrI	ACTGGG	1 cut(s)	2149
BmsI	GCATC	8 cut(s)	118, 276, 1209, 1447, 1451, 1580, 1656, 1972
BmuI	ACTGGG	1 cut(s)	2149
BpiI	GAAGAC	2 cut(s)	337, 555
BpmI	CTGGAG	1 cut(s)	2112
Bpu10I	CCTNAGC	1 cut(s)	2237
Bpu14I	TTCGAA	2 cut(s)	75, 2263
BpuEI	CTTGAG	3 cut(s)	354, 1196, 1202
BpuMI	CCSGG	7 cut(s)	324, 325, 543, 1447, 1448, 1676, 1677
Bsa29I	ATCGAT	1 cut(s)	1793
BsaAI	YACGTR	1 cut(s)	480
BsaHI	GRCGYC	2 cut(s)	865, 1398
BsaI	GGTCTC	4 cut(s)	504, 1107, 1503, 2103
BsaWI	WCCGGW	2 cut(s)	615, 940
BsaXI	ACNNNNNCTCC	6 cut(s)	716, 746, 922, 952, 2303, 2333
Bse118I	RCCGGY	5 cut(s)	615, 1316, 1667, 1919, 1959
Bse1I	ACTGG	5 cut(s)	674, 777, 1859, 2144, 2334
Bse3DI	GCAATG	5 cut(s)	164, 209, 456, 1698, 1791
BseAI	TCCGGA	1 cut(s)	940
BseBI	CCWGG	7 cut(s)	351, 1382, 1533, 1682, 1731, 1830, 2191
BseCI	ATCGAT	1 cut(s)	1793
BseGI	GGATG	5 cut(s)	756, 1107, 1442, 1462, 1571
BseMI	GCAATG	5 cut(s)	164, 209, 456, 1698, 1791
BseMII	CTCAG	1 cut(s)	912
BseNI	ACTGG	5 cut(s)	674, 777, 1859, 2144, 2334
BseRI	GAGGAG	3 cut(s)	35, 1199, 1778
BseSI	GKGCMC	7 cut(s)	323, 1011, 1142, 1227, 1583, 1881, 2146
BseYI	CCCAGC	1 cut(s)	1517
Bsh1236I	CGCG	6 cut(s)	528, 855, 1119, 1193, 1645, 1968
BshNI	GGYRCC	6 cut(s)	353, 864, 1755, 1824, 1922, 2143
BshTI	ACCGGT	1 cut(s)	615
BshVI	ATCGAT	1 cut(s)	1793
BsiHKAI	GWGCWC	1 cut(s)	1011
BsiHKCI	CYCGRG	4 cut(s)	323, 1097, 1446, 1675
BslFI	GGGAC	6 cut(s)	203, 239, 340, 797, 2218, 2292
BsmAI	GTCTC	8 cut(s)	504, 530, 1087, 1107, 1503, 2085, 2103, 2323
BsmBI	CGTCTC	1 cut(s)	2085
BsmFI	GGGAC	6 cut(s)	203, 239, 340, 797, 2218, 2292
Bso31I	GGTCTC	4 cut(s)	504, 1107, 1503, 2103
BsoBI	CYCGRG	4 cut(s)	323, 1097, 1446, 1675
Bsp119I	TTCGAA	2 cut(s)	75, 2263
Bsp120I	GGGCCC	4 cut(s)	319, 1138, 1223, 1579
Bsp1286I	GDGCHC	9 cut(s)	323, 491, 1011, 1142, 1227, 1583, 1881, 2146, 2197
Bsp13I	TCCGGA	1 cut(s)	940
Bsp1407I	TGTACA	1 cut(s)	453
Bsp143I	GATC	4 cut(s)	682, 1171, 1483, 2016
BspCNI	CTCAG	1 cut(s)	911
BspDI	ATCGAT	1 cut(s)	1793
BspEI	TCCGGA	1 cut(s)	940
BspFNI	CGCG	6 cut(s)	528, 855, 1119, 1193, 1645, 1968
BspMAI	CTGCAG	2 cut(s)	38, 1570
BspMI	ACCTGC	1 cut(s)	962
BspPI	GGATC	2 cut(s)	1179, 2011
BspT104I	TTCGAA	2 cut(s)	75, 2263
BspT107I	GGYRCC	6 cut(s)	353, 864, 1755, 1824, 1922, 2143
BspTNI	GGTCTC	4 cut(s)	504, 1107, 1503, 2103
BsrDI	GCAATG	5 cut(s)	164, 209, 456, 1698, 1791
BsrFI	RCCGGY	5 cut(s)	615, 1316, 1667, 1919, 1959
BsrGI	TGTACA	1 cut(s)	453
BsrI	ACTGG	5 cut(s)	674, 777, 1859, 2144, 2334
BssAI	RCCGGY	5 cut(s)	615, 1316, 1667, 1919, 1959
BssMI	GATC	4 cut(s)	682, 1171, 1483, 2016
BssNI	GRCGYC	2 cut(s)	865, 1398
BssSI	CACGAG	1 cut(s)	1704
BssT1I	CCWWGG	3 cut(s)	138, 262, 1575
Bst2BI	CACGAG	1 cut(s)	1704
Bst2UI	CCWGG	7 cut(s)	351, 1382, 1533, 1682, 1731, 1830, 2191
BstACI	GRCGYC	2 cut(s)	865, 1398
BstAPI	GCANNNNNTGC	1 cut(s)	1700
BstAUI	TGTACA	1 cut(s)	453
BstBAI	YACGTR	1 cut(s)	480
BstBI	TTCGAA	2 cut(s)	75, 2263
BstC8I	GCNNGC	8 cut(s)	18, 115, 472, 580, 713, 1669, 1961, 2031
BstDEI	CTNAG	5 cut(s)	330, 506, 898, 1907, 2237
BstDSI	CCRYGG	2 cut(s)	515, 1117
BstEII	GGTNACC	2 cut(s)	2161, 2300
BstF5I	GGATG	5 cut(s)	756, 1107, 1442, 1462, 1571
BstFNI	CGCG	6 cut(s)	528, 855, 1119, 1193, 1645, 1968
BstH2I	RGCGCY	1 cut(s)	868
BstHHI	GCGC	5 cut(s)	530, 867, 1195, 1647, 1970
BstKTI	GATC	4 cut(s)	685, 1174, 1486, 2019
BstMAI	GTCTC	8 cut(s)	504, 530, 1087, 1107, 1503, 2085, 2103, 2323
BstMBI	GATC	4 cut(s)	682, 1171, 1483, 2016
BstNI	CCWGG	7 cut(s)	351, 1382, 1533, 1682, 1731, 1830, 2191
BstNSI	RCATGY	2 cut(s)	1329, 2033
BstPI	GGTNACC	2 cut(s)	2161, 2300
BstSFI	CTRYAG	4 cut(s)	34, 1566, 1840, 1849
BstSLI	GKGCMC	7 cut(s)	323, 1011, 1142, 1227, 1583, 1881, 2146
BstSNI	TACGTA	1 cut(s)	480
BstUI	CGCG	6 cut(s)	528, 855, 1119, 1193, 1645, 1968
BstV2I	GAAGAC	2 cut(s)	337, 555
BstX2I	RGATCY	1 cut(s)	1171
BstXI	CCANNNNNNTGG	2 cut(s)	1688, 1955
BstYI	RGATCY	1 cut(s)	1171
Bsu15I	ATCGAT	1 cut(s)	1793
BsuI	GTATCC	1 cut(s)	1325
BsuTUI	ATCGAT	1 cut(s)	1793
BtgI	CCRYGG	2 cut(s)	515, 1117
BtgZI	GCGATG	2 cut(s)	1633, 1949
BtrI	CACGTC	3 cut(s)	1621, 1752, 2071
BtsCI	GGATG	5 cut(s)	756, 1107, 1442, 1462, 1571
BtsI	GCAGTG	2 cut(s)	240, 1029
BtsIMutI	CAGTG	7 cut(s)	240, 784, 906, 1029, 1866, 1932, 2058
BveI	ACCTGC	1 cut(s)	962
Cac8I	GCNNGC	8 cut(s)	18, 115, 472, 580, 713, 1669, 1961, 2031
CfoI	GCGC	5 cut(s)	530, 867, 1195, 1647, 1970
Cfr10I	RCCGGY	5 cut(s)	615, 1316, 1667, 1919, 1959
Cfr42I	CCGCGG	1 cut(s)	1120
Cfr9I	CCCGGG	3 cut(s)	323, 1446, 1675
ClaI	ATCGAT	1 cut(s)	1793
CseI	GACGC	2 cut(s)	745, 1448
Csp6I	GTAC	6 cut(s)	454, 851, 1257, 1756, 1825, 1923
CspAI	ACCGGT	1 cut(s)	615
CspCI	CAANNNNNGTGG	4 cut(s)	543, 578, 1921, 1956
CviAII	CATG	9 cut(s)	13, 721, 784, 861, 1326, 1801, 2030, 2222, 2308
CviQI	GTAC	6 cut(s)	454, 851, 1257, 1756, 1825, 1923
DdeI	CTNAG	5 cut(s)	330, 506, 898, 1907, 2237
DinI	GGCGCC	1 cut(s)	866
DpnI	GATC	4 cut(s)	684, 1173, 1485, 2018
DpnII	GATC	4 cut(s)	682, 1171, 1483, 2016
DriI	GACNNNNNGTC	2 cut(s)	1120, 1804
EaeI	YGGCCR	4 cut(s)	774, 1002, 1957, 2187
Eam1105I	GACNNNNNGTC	2 cut(s)	1120, 1804
Eco105I	TACGTA	1 cut(s)	480
Eco130I	CCWWGG	3 cut(s)	138, 262, 1575
Eco24I	GRGCYC	6 cut(s)	323, 491, 1142, 1227, 1583, 2197
Eco31I	GGTCTC	4 cut(s)	504, 1107, 1503, 2103
Eco47I	GGWCC	4 cut(s)	190, 288, 1122, 1546
Eco88I	CYCGRG	4 cut(s)	323, 1097, 1446, 1675
Eco91I	GGTNACC	2 cut(s)	2161, 2300
EcoO109I	RGGNCCY	7 cut(s)	190, 320, 1122, 1139, 1224, 1546, 1579
EcoO65I	GGTNACC	2 cut(s)	2161, 2300
EcoRI	GAATTC	3 cut(s)	1502, 2259, 2268
EcoRII	CCWGG	7 cut(s)	349, 1380, 1531, 1680, 1729, 1828, 2189
EcoT14I	CCWWGG	3 cut(s)	138, 262, 1575
EcoT22I	ATGCAT	1 cut(s)	1066
EcoT38I	GRGCYC	6 cut(s)	323, 491, 1142, 1227, 1583, 2197
EgeI	GGCGCC	1 cut(s)	866
EheI	GGCGCC	1 cut(s)	866
ErhI	CCWWGG	3 cut(s)	138, 262, 1575
Esp3I	CGTCTC	1 cut(s)	2085
FaeI	CATG	9 cut(s)	16, 724, 787, 864, 1329, 1804, 2033, 2225, 2311
FaqI	GGGAC	6 cut(s)	203, 239, 340, 797, 2218, 2292
FatI	CATG	9 cut(s)	12, 720, 783, 860, 1325, 1800, 2029, 2221, 2307
FauI	CCCGC	5 cut(s)	25, 1112, 1378, 1650, 2338
FbaI	TGATCA	1 cut(s)	1483
FokI	GGATG	5 cut(s)	763, 1114, 1429, 1469, 1558
FriOI	GRGCYC	6 cut(s)	323, 491, 1142, 1227, 1583, 2197
FspBI	CTAG	3 cut(s)	360, 702, 2111
GlaI	GCGC	5 cut(s)	529, 866, 1194, 1646, 1969
GsaI	CCCAGC	1 cut(s)	1521
GsuI	CTGGAG	1 cut(s)	2112
HaeII	RGCGCY	1 cut(s)	868
HgaI	GACGC	2 cut(s)	745, 1448
HhaI	GCGC	5 cut(s)	530, 867, 1195, 1647, 1970
Hin1I	GRCGYC	2 cut(s)	865, 1398
Hin1II	CATG	9 cut(s)	16, 724, 787, 864, 1329, 1804, 2033, 2225, 2311
Hin6I	GCGC	5 cut(s)	528, 865, 1193, 1645, 1968
HinP1I	GCGC	5 cut(s)	528, 865, 1193, 1645, 1968
HincII	GTYRAC	3 cut(s)	207, 1051, 2068
HindII	GTYRAC	3 cut(s)	207, 1051, 2068
HindIII	AAGCTT	1 cut(s)	1739
Hpy166II	GTNNAC	9 cut(s)	207, 238, 499, 1009, 1027, 1051, 1242, 2068, 2248
Hpy188III	TCNNGA	5 cut(s)	748, 941, 1099, 1169, 1213
Hpy8I	GTNNAC	9 cut(s)	207, 238, 499, 1009, 1027, 1051, 1242, 2068, 2248
Hpy99I	CGWCG	4 cut(s)	875, 1251, 1403, 1442
HpyAV	CCTTC	9 cut(s)	50, 89, 195, 203, 214, 403, 616, 758, 1036
HpyCH4IV	ACGT	9 cut(s)	479, 585, 731, 1149, 1398, 1620, 1751, 2064, 2070
HpyF3I	CTNAG	5 cut(s)	330, 506, 898, 1907, 2237
HpySE526I	ACGT	9 cut(s)	479, 585, 731, 1149, 1398, 1620, 1751, 2064, 2070
Hsp92I	GRCGYC	2 cut(s)	865, 1398
Hsp92II	CATG	9 cut(s)	16, 724, 787, 864, 1329, 1804, 2033, 2225, 2311
HspAI	GCGC	5 cut(s)	528, 865, 1193, 1645, 1968
KasI	GGCGCC	1 cut(s)	864
Kpn2I	TCCGGA	1 cut(s)	940
KpnI	GGTACC	3 cut(s)	1759, 1828, 1926
KroI	GCCGGC	2 cut(s)	1667, 1959
KroNI	GCCGGC	2 cut(s)	1669, 1961
Ksp22I	TGATCA	1 cut(s)	1483
KspI	CCGCGG	1 cut(s)	1120
Kzo9I	GATC	4 cut(s)	682, 1171, 1483, 2016
LmnI	GCTCC	3 cut(s)	486, 1236, 1691
LweI	GCATC	8 cut(s)	118, 276, 1209, 1447, 1451, 1580, 1656, 1972
MaeI	CTAG	3 cut(s)	360, 702, 2111
MaeII	ACGT	9 cut(s)	479, 585, 731, 1149, 1398, 1620, 1751, 2064, 2070
MaeIII	GTNAC	9 cut(s)	611, 1078, 1250, 1288, 1720, 2023, 2084, 2161, 2300
MalI	GATC	4 cut(s)	684, 1173, 1485, 2018
MboI	GATC	4 cut(s)	682, 1171, 1483, 2016
MboII	GAAGA	7 cut(s)	337, 560, 956, 1028, 1845, 2277, 2282
MfeI	CAATTG	1 cut(s)	1614
MflI	RGATCY	1 cut(s)	1171
MhlI	GDGCHC	9 cut(s)	323, 491, 1011, 1142, 1227, 1583, 1881, 2146, 2197
MlsI	TGGCCA	1 cut(s)	776
MluNI	TGGCCA	1 cut(s)	776
Mly113I	GGCGCC	1 cut(s)	865
MlyI	GAGTC	5 cut(s)	113, 532, 890, 1089, 1728
MmeI	TCCRAC	1 cut(s)	1921
Mox20I	TGGCCA	1 cut(s)	776
Mph1103I	ATGCAT	1 cut(s)	1066
MroI	TCCGGA	1 cut(s)	940
MroNI	GCCGGC	2 cut(s)	1667, 1959
MscI	TGGCCA	1 cut(s)	776
MseI	TTAA	6 cut(s)	27, 225, 696, 966, 1773, 2365
MslI	CAYNNNNRTG	3 cut(s)	696, 2222, 2298
Msp20I	TGGCCA	1 cut(s)	776
MspA1I	CMGCKG	5 cut(s)	386, 953, 959, 1119, 2158
MunI	CAATTG	1 cut(s)	1614
MvaI	CCWGG	7 cut(s)	351, 1382, 1533, 1682, 1731, 1830, 2191
MvnI	CGCG	6 cut(s)	528, 855, 1119, 1193, 1645, 1968
NaeI	GCCGGC	2 cut(s)	1669, 1961
NarI	GGCGCC	1 cut(s)	865
NciI	CCSGG	7 cut(s)	324, 325, 543, 1447, 1448, 1676, 1677
NdeII	GATC	4 cut(s)	682, 1171, 1483, 2016
NgoMIV	GCCGGC	2 cut(s)	1667, 1959
NlaIII	CATG	9 cut(s)	16, 724, 787, 864, 1329, 1804, 2033, 2225, 2311
NmuCI	GTSAC	4 cut(s)	1078, 1250, 1720, 2300
NsiI	ATGCAT	1 cut(s)	1066
NspI	RCATGY	2 cut(s)	1329, 2033
NspV	TTCGAA	2 cut(s)	75, 2263
OliI	CACNNNNGTG	1 cut(s)	2298
PaeI	GCATGC	1 cut(s)	2033
PcsI	WCGNNNNNNNCGW	3 cut(s)	426, 435, 2206
PdiI	GCCGGC	2 cut(s)	1669, 1961
PfeI	GAWTC	5 cut(s)	1216, 1845, 1944, 1997, 2211
PflFI	GACNNNGTC	1 cut(s)	2006
PflMI	CCANNNNNTGG	1 cut(s)	263
PinAI	ACCGGT	1 cut(s)	615
PleI	GAGTC	5 cut(s)	112, 532, 890, 1089, 1727
PluTI	GGCGCC	1 cut(s)	868
PpsI	GAGTC	5 cut(s)	112, 532, 890, 1089, 1727
Ppu21I	YACGTR	1 cut(s)	480
PpuMI	RGGWCCY	3 cut(s)	190, 1122, 1546
Psp5II	RGGWCCY	3 cut(s)	190, 1122, 1546
Psp6I	CCWGG	7 cut(s)	349, 1380, 1531, 1680, 1729, 1828, 2189
PspEI	GGTNACC	2 cut(s)	2161, 2300
PspFI	CCCAGC	1 cut(s)	1517
PspGI	CCWGG	7 cut(s)	349, 1380, 1531, 1680, 1729, 1828, 2189
PspOMI	GGGCCC	4 cut(s)	319, 1138, 1223, 1579
PspPPI	RGGWCCY	3 cut(s)	190, 1122, 1546
PstI	CTGCAG	2 cut(s)	38, 1570
PsuI	RGATCY	1 cut(s)	1171
PsyI	GACNNNGTC	1 cut(s)	2006
PvuII	CAGCTG	1 cut(s)	2158
RsaI	GTAC	6 cut(s)	455, 852, 1258, 1757, 1826, 1924
RsaNI	GTAC	6 cut(s)	454, 851, 1257, 1756, 1825, 1923
RseI	CAYNNNNRTG	3 cut(s)	696, 2222, 2298
SacII	CCGCGG	1 cut(s)	1120
SaqAI	TTAA	6 cut(s)	27, 225, 696, 966, 1773, 2365
Sau3AI	GATC	4 cut(s)	682, 1171, 1483, 2016
SchI	GAGTC	5 cut(s)	113, 532, 890, 1089, 1728
SduI	GDGCHC	9 cut(s)	323, 491, 1011, 1142, 1227, 1583, 1881, 2146, 2197
SfaNI	GCATC	8 cut(s)	118, 276, 1209, 1447, 1451, 1580, 1656, 1972
SfcI	CTRYAG	4 cut(s)	34, 1566, 1840, 1849
SfoI	GGCGCC	1 cut(s)	866
Sfr303I	CCGCGG	1 cut(s)	1120
SfuI	TTCGAA	2 cut(s)	75, 2263
SgrBI	CCGCGG	1 cut(s)	1120
SinI	GGWCC	4 cut(s)	190, 288, 1122, 1546
SmaI	CCCGGG	3 cut(s)	325, 1448, 1677
SmiMI	CAYNNNNRTG	3 cut(s)	696, 2222, 2298
SmlI	CTYRAG	3 cut(s)	369, 1181, 1211
SmoI	CTYRAG	3 cut(s)	369, 1181, 1211
SnaBI	TACGTA	1 cut(s)	480
SphI	GCATGC	1 cut(s)	2033
SspDI	GGCGCC	1 cut(s)	864
SspMI	CTAG	3 cut(s)	360, 702, 2111
StyI	CCWWGG	3 cut(s)	138, 262, 1575
TaiI	ACGT	9 cut(s)	482, 588, 734, 1152, 1401, 1623, 1754, 2067, 2073
TaqI	TCGA	7 cut(s)	75, 663, 735, 870, 1437, 1793, 2263
TaqII	GACCGA	1 cut(s)	1527
TatI	WGTACW	1 cut(s)	453
TauI	GCSGC	7 cut(s)	134, 528, 632, 953, 959, 1190, 1193
TfiI	GAWTC	5 cut(s)	1216, 1845, 1944, 1997, 2211
Tru1I	TTAA	6 cut(s)	27, 225, 696, 966, 1773, 2365
Tru9I	TTAA	6 cut(s)	27, 225, 696, 966, 1773, 2365
TscAI	CASTG	7 cut(s)	247, 784, 906, 1029, 1866, 1939, 2058
TseFI	GTSAC	4 cut(s)	1078, 1250, 1720, 2300
Tsp45I	GTSAC	4 cut(s)	1078, 1250, 1720, 2300
TspDTI	ATGAA	2 cut(s)	2210, 2328
TspGWI	ACGGA	3 cut(s)	318, 690, 1896
TspMI	CCCGGG	3 cut(s)	323, 1446, 1675
TspRI	CASTG	7 cut(s)	247, 784, 906, 1029, 1866, 1939, 2058
Tth111I	GACNNNGTC	1 cut(s)	2006
Van91I	CCANNNNNTGG	1 cut(s)	263
VneI	GTGCAC	1 cut(s)	1007
VpaK11BI	GGWCC	4 cut(s)	190, 288, 1122, 1546
XapI	RAATTY	5 cut(s)	1360, 1502, 1898, 2259, 2268
XceI	RCATGY	2 cut(s)	1329, 2033
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2136
XmaI	CCCGGG	3 cut(s)	323, 1446, 1675
XspI	CTAG	3 cut(s)	360, 702, 2111
ZraI	GACGTC	1 cut(s)	1399
Zsp2I	ATGCAT	1 cut(s)	1066

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.