FvH4_6g00030

Type II inositol 1,4,5-trisphosphate 5-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010730
Physical Location & Seq
Reverse (-)
25084 .. 34343
9260 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g00030.t2

Sequence Viewer

Length: 3216 bp
ATGGAAGAAAACGACGATTTCTTATCGTCTCCGATCGTCTTCGTGGACTCGTCACCGTCTTCATCGGAAGACGAAAATGACGCCGTCTCCCTCCATTCCACTTCAAAACGCCTCGACTATATGCTCCAATTCCTCGATCGCAAGCTCTCCGTCGACCACAGCGGCAACACGAGCAACGCCTCCCCGCTCCCTGAGTTCGAGGCCAAGGGCGGACGTACGGGAATGTTCAAAGTCCCGGTTCGGGCTGCGGTCCACCCGAACCGGCCTCCCAAGCTGGAACTGAGGCCCCAACCGCTGAGGGAGACTCAAATAGGATGCTTCTTGCGGACTATGGTCAGCACCGAGTCCCAATTGTGGGCTGGGACCGAGTGCGCCGTCAGGGTGTGGAACTTTAACGATTTGTACTCGGCGGCGGCCGCTGGACAAGGGAGAGGCGATGAGGAAACGGCACCGTTTAGGGAGTCCGTGTCCACATCCGCGGTCATGTGTTTAGTTGGGGACGAGGGGAATAGGGTGGTGTGGAGTGGGCATAGGGATGGTAGGATCAGGTGTTGGAGTATGGACTCCATAACTGCTCCTTTTAAGGACTGCTTGTCTTGGCAGGCTTATAGAGGCCCCGTTCTTTCTCTTGTTATTTCTTGTTATGGGGATCTCTGGTCAGGCTCAGAGGGTGGTGTCATCAAGATATGGCCATGGGAGGCTATTGAAAAAGCTCTCTCTCTCACCATTGAAGAAAGACTCGTGTCTTCTTTGTTGGTGGAGAGGTCTTACATTGATCCTTGGACCCAAGTTGCCGTAAATGGTTTCACTAATGTACTAACTTGGGATGTTAGGTACTTATTATCAGATAGTTCCAGTGCAAAGGTGTGGAGTGCTGGTTATCTCTCATTTGCACTCTGGGATGCGCGTACAAGAGAATTACTGAAAGTATTCAATACCGACGGTCTGAATGAGAATCGAGTGGACATTTCATTGGCACAGGAGTTGCCAGTAGAATTAATTTCCGGAGCAAAAAAAGATAAAACACAAAGTTCTTTTGGTTTCTTTCAGCGATCACGTAATGCATTAATGGGCGCAGCAGATGCTGTTCGTCGAGTTGCAGTGAAGGGTTCATTTGGAGATGATAATCGAAGAACTGAAGCATTGGTAATTGCAGTAGATACAATGATTTGGACGGGGTGTACAAATGGCCTACTTGTGCAGTGGGATAGAAATGGCAACCGTATGCAAGAATTCCATTACCATTCTTCTGCTGTTCAATGCTTTTGTACTTTTGGGCTACGTATATGGGTTGGTTATGCAAGTGGCACTGTCCAAGTATTGGACCTTGACGGTAATCTGCTCGGAGGATGGGTTGCTGAAAACTGTTCTATTATTAAAATAGCTACAGGAGCTGGTTATGTATTTACCTTGGCCAATCATGGTGGTATATGTGGATGGAATATTACATCACCGGGACCTCTTGACAGCATAGTGCGTTCAGAATTAGCTGGCAAAGAATTTCTATATACAAGGATAGAAAACATGAAAATTTTGACTGGTACATGGAATGTTGGACAAGGAAGAGCATCCCAGGACTCACTTATATCCTGGCTAGGCTCTGTGGCTTCTAATGTTGGCATTGTTGTTGTTGGTCTGCAAGAAGTTGAAATGGGCGCTGGTTTTCTTGCTATGTCTGCAGCCAAAGAAACTGTAGGTCTTGAGGGGAGCTCTGTTGGGCAGTGGTGGCTGGATATGATTGGAAAAACATTGGATGAAGGATCAACCTTTGAACGTGTTGGATCTAGACAGTTGGCAGGATTGCTCATTGCTATGTGGGTGAGACATAATCTCAAAGCTCACGTTGGGGATGTTGATGCTGCAGCAGTTCCATGTGGTTTTGGGCGTGCTATTGGTAACAAGGGAGCTGTTGGTTTGAGGATTAGATTGTATGGCAGGACAATGTGCTTTGTTAATTGTCACTTTGCTGCGCATTTGGAAGCCGTTAATCGCCGCAATGCAGATTTCGATCATGTATATCGAACCATGACCTTCTCTCGGCCGAATTATCTCAATTGTGCAGCTGCTACTGCTTCATCTGCTGTCCAATTGCCTCGTGGTACAAATGCTATTGGTAACAATTCTGTAGAAGGGATGCCTGAGTTATCTGAAGCAGACATGGTCATATTCCTTGGTGATTTTAACTATCGGCTTGATGGTATATCTTATGATGAAGCTAGAGATTTTGTTTCTCAAAGATGTTTTGATTGGCTTAGAGAACGGGATCAGCTCCGAGTGGAAATGACAGCCGGAAATGTCTTTCAAGGAATGCGGGAGGCAGAGATTACATTTCCTCCCACATACAAGTTTGAAAGGCATCAAGCTGGTTTAGCAGGGTATGATTCTGGTGAAAAGAAGCGCATTCCAGCTTGGTGTGATAGAATTCTGTATCGTGATAGCCGCTCAGCGTTGGTATCAGAATGCTGCTTAGGATGTCCTGTTGTCTCTTCAATATCACGGTACGAGGCTTCCATGGATGTAACTGACAGTGATCACAAGCCAGTCCGGTGCATTTTTACTCTTGATATTGCTCGAGTTGATGAGTCAATAAGGAGACAAGAGCTTGGAGATATCCTGGAATCAAATGGGAAGCTCAAATGTATGCTTGAAGAACTATCCAAGATTCCTGAAACTATTGTCAGTACAAACAAAATAATCCTTCAAAACCAGGATACATCCATTTTGCGTATTACAAATAAATCTGGCCAAAAAGATGCTTTTTTCGAAATAATTTGTGAAGGGCAATCTGTCATTAAGGAGGATGGACATGCATCAGATCATTGTCCCAGAGGTTCCTTTGGCTTTCCGCGATGGCTTCAGGTCACTCCTGCAGCTGGCATTATCAGACCAGATCATATAGCAGAGGTGTCTGTTCATCATGAGGAACACCAAACTCTAAAAGAGTTTGTCGATGGTGTCCCCCAGAACCGTTGGTGTGAAAACACCAGAGACAAGGAAGTTATACTGGTGGTTAAGGTACATGGGAGGTACACTAATAACACCAAAAGCCATCGCGTCTGTGTGCGTCACTGCTGTTCAGCCAATACAAAGCAAAGAGAACCCCCCGAGCACGACACCAGACAAACCCAAGGAACTGCTCTGCTCCGGTCTAACTTCCAACACCTTAGCAGTTCCTATGACGTGGTTGACCACCTGTGGGGTATGAATAGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

1072

Amino Acids

118.91

Weight (kDa)

5.91

Isoelectric Point (pI)

38.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_IP5PC_F PF23754 99 - 480 2.3e-180 IP5P C-F beta-propeller
Exo_endo_phos2 PF22669 514 - 849 2.2e-66 Endonuclease/Exonuclease/phosphatase family 2
Exo_endo_phos PF03372 514 - 845 3.7e-07 Endonuclease/Exonuclease/phosphatase family
Ig-like_IP5PC_F PF23755 888 - 1024 6.1e-70 IP5P C-F Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010730)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65580 AT1G65580
fragaria_vesca FvH4_6g00030 FvH4_6g00030
malus_domestica MD12G1260800.v1.1
prunus_persica Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1
pyrus_communis pycom12g24280
rosa_chinensis RchiOBHm_Chr3g0447351
rosa_laevigata RLG00000026007
rosa_multiflora Rmu_ssc0000473.1_g000002
rosa_roxburghii Rroxscaffold_6G00425530
rosa_rugosa Rorug02G0600200 Rorug02G0600300
rosa_samantha Rh3AG000500 Rh3BG000300 Rh3CG000300 Rh3DG000500
rosa_wichuraiana Rw3G000030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1971
AccB1I GGYRCC 1 cut(s) 448
AccB7I CCANNNNNTGG 1 cut(s) 1321
AccBSI CCGCTC 2 cut(s) 187, 2442
AccI GTMKAC 1 cut(s) 153
AccII CGCG 4 cut(s) 479, 907, 2848, 3054
AccIII TCCGGA 1 cut(s) 1004
AclWI GGATC 6 cut(s) 551, 657, 770, 1768, 1789, 2271
AcoI YGGCCR 5 cut(s) 414, 689, 1413, 2039, 2743
AcsI RAATTY 4 cut(s) 1232, 1499, 1530, 2421
AcuI CTGAAG 3 cut(s) 1158, 2169, 2840
AcyI GRCGYC 1 cut(s) 81
AfiI CCNNNNNNNGG 7 cut(s) 241, 354, 355, 1321, 2037, 2873, 3196
AflIII ACRYGT 1 cut(s) 1774
AhdI GACNNNNNGTC 1 cut(s) 592
AjiI CACGTC 1 cut(s) 3181
AjnI CCWGG 4 cut(s) 1572, 1589, 2613, 2706
Alw21I GWGCWC 2 cut(s) 1712, 3111
Alw26I GTCTC 7 cut(s) 33, 91, 296, 1816, 2488, 2587, 2982
AlwI GGATC 6 cut(s) 551, 657, 770, 1768, 1789, 2271
AlwNI CAGNNNCTG 2 cut(s) 1085, 1394
Ama87I CYCGRG 2 cut(s) 2571, 3104
Aor13HI TCCGGA 1 cut(s) 1004
ApoI RAATTY 4 cut(s) 1232, 1499, 1530, 2421
AseI ATTAAT 2 cut(s) 998, 1067
Asp700I GAANNNNTTC 1 cut(s) 929
AspLEI GCGC 6 cut(s) 374, 907, 1076, 1658, 1972, 2400
AspS9I GGNCC 7 cut(s) 250, 285, 363, 614, 783, 1324, 1457
AsuC2I CCSGG 2 cut(s) 236, 1455
AsuHPI GGTGA 6 cut(s) 45, 715, 1443, 1831, 2186, 2399
AsuII TTCGAA 1 cut(s) 2763
AvaI CYCGRG 2 cut(s) 2571, 3104
AvaII GGWCC 5 cut(s) 250, 363, 783, 1324, 1457
BalI TGGCCA 3 cut(s) 691, 1415, 2745
BanI GGYRCC 1 cut(s) 448
BanII GRGCYC 1 cut(s) 1712
BauI CACGAG 3 cut(s) 169, 740, 2094
BbsI GAAGAC 4 cut(s) 31, 51, 75, 738
Bbv12I GWGCWC 2 cut(s) 1712, 3111
BbvCI CCTCAGC 1 cut(s) 296
BccI CCATC 8 cut(s) 530, 1344, 1431, 2189, 2795, 2844, 2945, 3057
BceAI ACGGC 5 cut(s) 68, 359, 462, 779, 1967
BciT130I CCWGG 4 cut(s) 1574, 1591, 2615, 2708
BciVI GTATCC 1 cut(s) 2704
BclI TGATCA 1 cut(s) 2530
BcnI CCSGG 2 cut(s) 236, 1455
BcoDI GTCTC 7 cut(s) 33, 91, 296, 1816, 2488, 2587, 2982
BfaI CTAG 3 cut(s) 1595, 1785, 2217
BfmI CTRYAG 6 cut(s) 1386, 1677, 1692, 1860, 2124, 2868
BfoI RGCGCY 1 cut(s) 1659
BfuI GTATCC 1 cut(s) 2704
BlpI GCTNAGC 1 cut(s) 2443
Bme1390I CCNGG 6 cut(s) 236, 1455, 1574, 1591, 2615, 2708
Bme18I GGWCC 5 cut(s) 250, 363, 783, 1324, 1457
BmeRI GACNNNNNGTC 1 cut(s) 592
BmeT110I CYCGRG 2 cut(s) 2571, 3104
BmgBI CACGTC 1 cut(s) 3181
BmgT120I GGNCC 7 cut(s) 250, 285, 363, 614, 783, 1324, 1457
BmiI GGNNCC 7 cut(s) 287, 364, 450, 616, 785, 1458, 2833
BmrFI CCNGG 6 cut(s) 236, 1455, 1574, 1591, 2615, 2708
BmsI GCATC 9 cut(s) 305, 892, 1072, 1577, 1846, 2124, 2365, 2743, 2819
BoxI GACNNNNGTC 2 cut(s) 332, 742
BpiI GAAGAC 4 cut(s) 31, 51, 75, 738
BplI GAGNNNNNCTC 4 cut(s) 289, 321, 1694, 1726
Bpu10I CCTNAGC 3 cut(s) 296, 2467, 3164
Bpu1102I GCTNAGC 1 cut(s) 2443
Bpu14I TTCGAA 1 cut(s) 2763
BpuEI CTTGAG 1 cut(s) 1721
BpuMI CCSGG 2 cut(s) 236, 1455
BsaAI YACGTR 2 cut(s) 1058, 1283
BsaBI GATNNNNATC 2 cut(s) 1125, 2007
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 9 cut(s) 204, 477, 692, 779, 1410, 1572, 2170, 2511, 3127
BsaWI WCCGGW 3 cut(s) 1004, 2544, 3144
Bsc4I CCNNNNNNNGG 7 cut(s) 241, 354, 355, 1321, 2037, 2873, 3196
Bse118I RCCGGY 1 cut(s) 261
Bse1I ACTGG 5 cut(s) 855, 989, 1543, 2540, 3009
Bse3DI GCAATG 2 cut(s) 1806, 2002
Bse8I GATNNNNATC 2 cut(s) 1125, 2007
BseAI TCCGGA 1 cut(s) 1004
BseBI CCWGG 4 cut(s) 1574, 1591, 2615, 2708
BseDI CCNNGG 9 cut(s) 204, 477, 692, 779, 1410, 1572, 2170, 2511, 3127
BseJI GATNNNNATC 2 cut(s) 1125, 2007
BseLI CCNNNNNNNGG 7 cut(s) 241, 354, 355, 1321, 2037, 2873, 3196
BseMI GCAATG 2 cut(s) 1806, 2002
BseMII CTCAG 6 cut(s) 183, 272, 287, 678, 2130, 2457
BseNI ACTGG 5 cut(s) 855, 989, 1543, 2540, 3009
BseX3I CGGCCG 2 cut(s) 414, 2039
BseYI CCCAGC 1 cut(s) 359
BsgI GTGCAG 2 cut(s) 1220, 2079
Bsh1236I CGCG 4 cut(s) 479, 907, 2848, 3054
Bsh1285I CGRYCG 4 cut(s) 36, 139, 417, 2042
BshNI GGYRCC 1 cut(s) 448
BsiEI CGRYCG 4 cut(s) 36, 139, 417, 2042
BsiHKAI GWGCWC 2 cut(s) 1712, 3111
BsiHKCI CYCGRG 2 cut(s) 2571, 3104
BsiSI CCGG 7 cut(s) 236, 262, 1005, 1454, 2289, 2545, 3145
BsiWI CGTACG 1 cut(s) 215
BslFI GGGAC 7 cut(s) 218, 331, 376, 512, 1470, 2808, 2942
BslI CCNNNNNNNGG 7 cut(s) 241, 354, 355, 1321, 2037, 2873, 3196
BsmAI GTCTC 7 cut(s) 33, 91, 296, 1816, 2488, 2587, 2982
BsmBI CGTCTC 2 cut(s) 33, 91
BsmFI GGGAC 7 cut(s) 218, 331, 376, 512, 1470, 2808, 2942
BsmI GAATGC 3 cut(s) 2313, 2400, 2465
BsoBI CYCGRG 2 cut(s) 2571, 3104
Bsp119I TTCGAA 1 cut(s) 2763
Bsp1286I GDGCHC 2 cut(s) 1712, 3111
Bsp13I TCCGGA 1 cut(s) 1004
Bsp1407I TGTACA 1 cut(s) 1181
Bsp1720I GCTNAGC 1 cut(s) 2443
Bsp19I CCATGG 2 cut(s) 692, 2511
BspCNI CTCAG 6 cut(s) 184, 273, 288, 677, 2131, 2456
BspEI TCCGGA 1 cut(s) 1004
BspFNI CGCG 4 cut(s) 479, 907, 2848, 3054
BspHI TCATGA 1 cut(s) 2917
BspLI GGNNCC 7 cut(s) 287, 364, 450, 616, 785, 1458, 2833
BspMAI CTGCAG 3 cut(s) 1681, 1864, 2872
BspPI GGATC 6 cut(s) 551, 657, 770, 1768, 1789, 2271
BspQI GCTCTTC 1 cut(s) 1558
BspT104I TTCGAA 1 cut(s) 2763
BspT107I GGYRCC 1 cut(s) 448
BsrBI CCGCTC 2 cut(s) 187, 2442
BsrDI GCAATG 2 cut(s) 1806, 2002
BsrFI RCCGGY 1 cut(s) 261
BsrGI TGTACA 1 cut(s) 1181
BsrI ACTGG 5 cut(s) 855, 989, 1543, 2540, 3009
BssAI RCCGGY 1 cut(s) 261
BssECI CCNNGG 9 cut(s) 204, 477, 692, 779, 1410, 1572, 2170, 2511, 3127
BssNI GRCGYC 1 cut(s) 81
BssSI CACGAG 3 cut(s) 169, 740, 2094
BssT1I CCWWGG 7 cut(s) 204, 692, 779, 1410, 2170, 2511, 3127
Bst2BI CACGAG 3 cut(s) 169, 740, 2094
Bst2UI CCWGG 4 cut(s) 1574, 1591, 2615, 2708
Bst6I CTCTTC 2 cut(s) 1558, 2491
BstACI GRCGYC 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 1082
BstAUI TGTACA 1 cut(s) 1181
BstBAI YACGTR 2 cut(s) 1058, 1283
BstBI TTCGAA 1 cut(s) 2763
BstC8I GCNNGC 5 cut(s) 143, 603, 1492, 1887, 2875
BstDEI CTNAG 9 cut(s) 192, 281, 296, 664, 2139, 2252, 2443, 2467, 3164
BstDSI CCRYGG 3 cut(s) 477, 692, 2511
BstFNI CGCG 4 cut(s) 479, 907, 2848, 3054
BstH2I RGCGCY 1 cut(s) 1659
BstHHI GCGC 6 cut(s) 374, 907, 1076, 1658, 1972, 2400
BstMAI GTCTC 7 cut(s) 33, 91, 296, 1816, 2488, 2587, 2982
BstMCI CGRYCG 4 cut(s) 36, 139, 417, 2042
BstNI CCWGG 4 cut(s) 1574, 1591, 2615, 2708
BstNSI RCATGY 1 cut(s) 2810
BstPAI GACNNNNGTC 2 cut(s) 332, 742
BstSCI CCNGG 6 cut(s) 234, 1453, 1572, 1589, 2613, 2706
BstSFI CTRYAG 6 cut(s) 1386, 1677, 1692, 1860, 2124, 2868
BstSNI TACGTA 1 cut(s) 1283
BstUI CGCG 4 cut(s) 479, 907, 2848, 3054
BstV2I GAAGAC 4 cut(s) 31, 51, 75, 738
BstX2I RGATCY 2 cut(s) 649, 1781
BstYI RGATCY 2 cut(s) 649, 1781
BstZI CGGCCG 2 cut(s) 414, 2039
BsuI GTATCC 1 cut(s) 2704
BtgI CCRYGG 3 cut(s) 477, 692, 2511
BtgZI GCGATG 3 cut(s) 450, 2863, 3035
BtrI CACGTC 1 cut(s) 3181
BtsI GCAGTG 4 cut(s) 1107, 1208, 1727, 3067
BtsIMutI CAGTG 7 cut(s) 862, 1107, 1208, 1308, 1727, 2533, 3067
Cac8I GCNNGC 5 cut(s) 143, 603, 1492, 1887, 2875
CaiI CAGNNNCTG 2 cut(s) 1085, 1394
CciI TCATGA 1 cut(s) 2917
CciNI GCGGCCGC 1 cut(s) 414
CfoI GCGC 6 cut(s) 374, 907, 1076, 1658, 1972, 2400
Cfr10I RCCGGY 1 cut(s) 261
Cfr13I GGNCC 7 cut(s) 250, 285, 363, 614, 783, 1324, 1457
Cfr42I CCGCGG 1 cut(s) 480
CseI GACGC 3 cut(s) 89, 3043, 3053
CspCI CAANNNNNGTGG 2 cut(s) 1405, 1440
DdeI CTNAG 9 cut(s) 192, 281, 296, 664, 2139, 2252, 2443, 2467, 3164
DriI GACNNNNNGTC 1 cut(s) 592
EaeI YGGCCR 5 cut(s) 414, 689, 1413, 2039, 2743
EagI CGGCCG 2 cut(s) 414, 2039
Eam1104I CTCTTC 2 cut(s) 1558, 2491
Eam1105I GACNNNNNGTC 1 cut(s) 592
EarI CTCTTC 2 cut(s) 1558, 2491
EciI GGCGGA 1 cut(s) 225
Ecl136II GAGCTC 1 cut(s) 1710
EclXI CGGCCG 2 cut(s) 414, 2039
Eco105I TACGTA 1 cut(s) 1283
Eco130I CCWWGG 7 cut(s) 204, 692, 779, 1410, 2170, 2511, 3127
Eco24I GRGCYC 1 cut(s) 1712
Eco32I GATATC 1 cut(s) 2611
Eco47I GGWCC 5 cut(s) 250, 363, 783, 1324, 1457
Eco52I CGGCCG 2 cut(s) 414, 2039
Eco53kI GAGCTC 1 cut(s) 1710
Eco57I CTGAAG 3 cut(s) 1158, 2169, 2840
Eco88I CYCGRG 2 cut(s) 2571, 3104
EcoICRI GAGCTC 1 cut(s) 1710
EcoO109I RGGNCCY 3 cut(s) 285, 614, 1457
EcoRI GAATTC 2 cut(s) 1232, 2421
EcoRII CCWGG 4 cut(s) 1572, 1589, 2613, 2706
EcoRV GATATC 1 cut(s) 2611
EcoT14I CCWWGG 7 cut(s) 204, 692, 779, 1410, 2170, 2511, 3127
EcoT22I ATGCAT 2 cut(s) 1066, 2812
EcoT38I GRGCYC 1 cut(s) 1712
ErhI CCWWGG 7 cut(s) 204, 692, 779, 1410, 2170, 2511, 3127
Esp3I CGTCTC 2 cut(s) 33, 91
FalI AAGNNNNNCTT 2 cut(s) 575, 607
FaqI GGGAC 7 cut(s) 218, 331, 376, 512, 1470, 2808, 2942
FauI CCCGC 2 cut(s) 192, 2304
FbaI TGATCA 1 cut(s) 2530
FblI GTMKAC 1 cut(s) 153
FriOI GRGCYC 1 cut(s) 1712
FspBI CTAG 3 cut(s) 1595, 1785, 2217
FspI TGCGCA 1 cut(s) 1971
GlaI GCGC 6 cut(s) 373, 906, 1075, 1657, 1971, 2399
GsaI CCCAGC 1 cut(s) 363
HaeII RGCGCY 1 cut(s) 1659
HapII CCGG 7 cut(s) 236, 262, 1005, 1454, 2289, 2545, 3145
HgaI GACGC 3 cut(s) 89, 3043, 3053
HhaI GCGC 6 cut(s) 374, 907, 1076, 1658, 1972, 2400
Hin1I GRCGYC 1 cut(s) 81
Hin6I GCGC 6 cut(s) 372, 905, 1074, 1656, 1970, 2398
HinP1I GCGC 6 cut(s) 372, 905, 1074, 1656, 1970, 2398
HincII GTYRAC 2 cut(s) 154, 3187
HindII GTYRAC 2 cut(s) 154, 3187
HpaII CCGG 7 cut(s) 236, 262, 1005, 1454, 2289, 2545, 3145
HphI GGTGA 6 cut(s) 45, 715, 1443, 1831, 2186, 2399
Hpy166II GTNNAC 8 cut(s) 46, 154, 253, 471, 964, 1182, 3030, 3187
Hpy188III TCNNGA 9 cut(s) 682, 1005, 1463, 1700, 1785, 2432, 2561, 2666, 2918
Hpy8I GTNNAC 8 cut(s) 46, 154, 253, 471, 964, 1182, 3030, 3187
Hpy99I CGWCG 4 cut(s) 17, 155, 944, 1095
HpyAV CCTTC 6 cut(s) 1099, 1751, 2041, 2123, 2708, 2771
HpyCH4IV ACGT 6 cut(s) 214, 1057, 1282, 1774, 1842, 3180
HpyF3I CTNAG 9 cut(s) 192, 281, 296, 664, 2139, 2252, 2443, 2467, 3164
HpySE526I ACGT 6 cut(s) 214, 1057, 1282, 1774, 1842, 3180
Hsp92I GRCGYC 1 cut(s) 81
HspAI GCGC 6 cut(s) 372, 905, 1074, 1656, 1970, 2398
Kpn2I TCCGGA 1 cut(s) 1004
Ksp22I TGATCA 1 cut(s) 2530
KspI CCGCGG 1 cut(s) 480
LguI GCTCTTC 1 cut(s) 1558
LmnI GCTCC 9 cut(s) 129, 192, 580, 1007, 1391, 1707, 1904, 2274, 3147
LweI GCATC 9 cut(s) 305, 892, 1072, 1577, 1846, 2124, 2365, 2743, 2819
MaeI CTAG 3 cut(s) 1595, 1785, 2217
MaeII ACGT 6 cut(s) 214, 1057, 1282, 1774, 1842, 3180
MaeIII GTNAC 7 cut(s) 51, 1895, 1958, 2114, 2518, 2860, 3065
MbiI CCGCTC 2 cut(s) 187, 2442
MfeI CAATTG 3 cut(s) 350, 2053, 2087
MflI RGATCY 2 cut(s) 649, 1781
MhlI GDGCHC 2 cut(s) 1712, 3111
MlsI TGGCCA 3 cut(s) 691, 1415, 2745
MluNI TGGCCA 3 cut(s) 691, 1415, 2745
MlyI GAGTC 8 cut(s) 41, 298, 353, 470, 557, 732, 1571, 2591
MmeI TCCRAC 4 cut(s) 533, 1534, 1759, 3181
Mox20I TGGCCA 3 cut(s) 691, 1415, 2745
Mph1103I ATGCAT 2 cut(s) 1066, 2812
MroI TCCGGA 1 cut(s) 1004
MroXI GAANNNNTTC 1 cut(s) 929
MscI TGGCCA 3 cut(s) 691, 1415, 2745
MslI CAYNNNNRTG 2 cut(s) 534, 1811
Msp20I TGGCCA 3 cut(s) 691, 1415, 2745
MspA1I CMGCKG 6 cut(s) 162, 295, 419, 479, 2063, 2873
MspI CCGG 7 cut(s) 236, 262, 1005, 1454, 2289, 2545, 3145
MspR9I CCNGG 6 cut(s) 236, 1455, 1574, 1591, 2615, 2708
MunI CAATTG 3 cut(s) 350, 2053, 2087
Mva1269I GAATGC 3 cut(s) 2313, 2400, 2465
MvaI CCWGG 4 cut(s) 1574, 1591, 2615, 2708
MvnI CGCG 4 cut(s) 479, 907, 2848, 3054
NciI CCSGG 2 cut(s) 236, 1455
NcoI CCATGG 2 cut(s) 692, 2511
NlaIV GGNNCC 7 cut(s) 287, 364, 450, 616, 785, 1458, 2833
NmeAIII GCCGAG 2 cut(s) 386, 2017
NmuCI GTSAC 4 cut(s) 51, 1958, 2860, 3065
NotI GCGGCCGC 1 cut(s) 414
NsbI TGCGCA 1 cut(s) 1971
NsiI ATGCAT 2 cut(s) 1066, 2812
NspI RCATGY 1 cut(s) 2810
NspV TTCGAA 1 cut(s) 2763
PaeR7I CTCGAG 1 cut(s) 2571
PagI TCATGA 1 cut(s) 2917
PciSI GCTCTTC 1 cut(s) 1558
PctI GAATGC 3 cut(s) 2313, 2400, 2465
PdmI GAANNNNTTC 1 cut(s) 929
PfeI GAWTC 4 cut(s) 955, 2381, 2618, 2662
Pfl23II CGTACG 1 cut(s) 215
PflFI GACNNNGTC 2 cut(s) 83, 2159
PflMI CCANNNNNTGG 1 cut(s) 1321
PfoI TCCNGGA 1 cut(s) 2613
Ple19I CGATCG 2 cut(s) 36, 139
PleI GAGTC 8 cut(s) 41, 298, 352, 469, 557, 732, 1571, 2590
PpsI GAGTC 8 cut(s) 41, 298, 352, 469, 557, 732, 1571, 2590
Ppu21I YACGTR 2 cut(s) 1058, 1283
PpuMI RGGWCCY 1 cut(s) 1457
PshAI GACNNNNGTC 2 cut(s) 332, 742
PshBI ATTAAT 2 cut(s) 998, 1067
Psp124BI GAGCTC 1 cut(s) 1712
Psp5II RGGWCCY 1 cut(s) 1457
Psp6I CCWGG 4 cut(s) 1572, 1589, 2613, 2706
PspFI CCCAGC 1 cut(s) 359
PspGI CCWGG 4 cut(s) 1572, 1589, 2613, 2706
PspLI CGTACG 1 cut(s) 215
PspN4I GGNNCC 7 cut(s) 287, 364, 450, 616, 785, 1458, 2833
PspPI GGNCC 7 cut(s) 250, 285, 363, 614, 783, 1324, 1457
PspPPI RGGWCCY 1 cut(s) 1457
PspXI VCTCGAGB 1 cut(s) 2571
PstI CTGCAG 3 cut(s) 1681, 1864, 2872
PstNI CAGNNNCTG 2 cut(s) 1085, 1394
PsuI RGATCY 2 cut(s) 649, 1781
PsyI GACNNNGTC 2 cut(s) 83, 2159
PvuI CGATCG 2 cut(s) 36, 139
PvuII CAGCTG 2 cut(s) 2063, 2873
RseI CAYNNNNRTG 2 cut(s) 534, 1811
SacI GAGCTC 1 cut(s) 1712
SacII CCGCGG 1 cut(s) 480
SalI GTCGAC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 1558
Sau96I GGNCC 7 cut(s) 250, 285, 363, 614, 783, 1324, 1457
SchI GAGTC 8 cut(s) 41, 298, 353, 470, 557, 732, 1571, 2591
ScrFI CCNGG 6 cut(s) 236, 1455, 1574, 1591, 2615, 2708
SduI GDGCHC 2 cut(s) 1712, 3111
SfaNI GCATC 9 cut(s) 305, 892, 1072, 1577, 1846, 2124, 2365, 2743, 2819
SfcI CTRYAG 6 cut(s) 1386, 1677, 1692, 1860, 2124, 2868
Sfr274I CTCGAG 1 cut(s) 2571
Sfr303I CCGCGG 1 cut(s) 480
SfuI TTCGAA 1 cut(s) 2763
SgrBI CCGCGG 1 cut(s) 480
SinI GGWCC 5 cut(s) 250, 363, 783, 1324, 1457
SlaI CTCGAG 1 cut(s) 2571
SmiMI CAYNNNNRTG 2 cut(s) 534, 1811
SmlI CTYRAG 2 cut(s) 1700, 2571
SmoI CTYRAG 2 cut(s) 1700, 2571
SnaBI TACGTA 1 cut(s) 1283
SspI AATATT 1 cut(s) 1444
SspMI CTAG 3 cut(s) 1595, 1785, 2217
SstI GAGCTC 1 cut(s) 1712
StyD4I CCNGG 6 cut(s) 234, 1453, 1572, 1589, 2613, 2706
StyI CCWWGG 7 cut(s) 204, 692, 779, 1410, 2170, 2511, 3127
TaiI ACGT 6 cut(s) 217, 1060, 1285, 1777, 1845, 3183
TaqII GACCGA 1 cut(s) 380
TatI WGTACW 5 cut(s) 402, 814, 1181, 1268, 2681
TauI GCSGC 6 cut(s) 165, 413, 416, 419, 1995, 2442
TfiI GAWTC 4 cut(s) 955, 2381, 2618, 2662
TscAI CASTG 7 cut(s) 862, 1107, 1208, 1315, 1727, 2533, 3074
TseFI GTSAC 4 cut(s) 51, 1958, 2860, 3065
Tsp45I GTSAC 4 cut(s) 51, 1958, 2860, 3065
TspDTI ATGAA 8 cut(s) 51, 960, 1101, 1541, 1770, 2064, 2226, 2903
TspGWI ACGGA 2 cut(s) 139, 454
TspRI CASTG 7 cut(s) 862, 1107, 1208, 1315, 1727, 2533, 3074
Tth111I GACNNNGTC 2 cut(s) 83, 2159
Van91I CCANNNNNTGG 1 cut(s) 1321
VpaK11BI GGWCC 5 cut(s) 250, 363, 783, 1324, 1457
VspI ATTAAT 2 cut(s) 998, 1067
XapI RAATTY 4 cut(s) 1232, 1499, 1530, 2421
XbaI TCTAGA 1 cut(s) 1784
XceI RCATGY 1 cut(s) 2810
XcmI CCANNNNNNNNNTGG 2 cut(s) 356, 2093
XhoI CTCGAG 1 cut(s) 2571
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 929
XspI CTAG 3 cut(s) 1595, 1785, 2217
Zsp2I ATGCAT 2 cut(s) 1066, 2812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.