Rh3AG000500

Type II inositol 1,4,5-trisphosphate 5-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0010730
Physical Location & Seq
Reverse (-)
61860 .. 71958
10099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG000500.1

Sequence Viewer

Length: 2640 bp
ATGGAAGAAAACGACGGTTTCTTATCGTCTCTGAGCCTCCGTAACTCCTCCGCGCCAATACCAAGACCAAACCAAAACGATGGTCTCGACGGCGGCAAACTCTTCGTGGACTCTTCACCATCTTCATCGGAAGATGAAAATGACGCCGCCTCCCTCCACTCCACTTCAAAACGCCTCGACTATATGCTCCAATTCCTCGACCGCAAGCTCTCCGTTGCCAACGTCAACGTCGTCGACCACATCAACAATATCAATATGAGCGACGCCGCCGACACTGCTCACGGCTCCTCCTCGCTCCCTGAGTTCGAGGCCAAGGGCGGAGGTACCGGAATGTTCAAAGTCCCGGTTCGGGGGGCGGTCCACCCGAACCGGCCGCCAAGGCTGGAACTGAGGCCCCAACCGCTGAGGGAGACTCAGATAGGATGCTTCTTGCGGACTATGGTCAGCACCGAGTCCCAATTGTGGGCTGGGACGGAGTGCGCCGTGAGGGTTTGGAATTTTAAAGATTTGTACTCGCCGGCGGCGGCTGAGGCTGGACGAGGGGAAGGCGATTCGGAAACGGCACCGTTTAGGGAGTCCGTGTGCACATCCGCGGTGATGTGTTTAGTTGGGGATGATGGGAATAGGGTGGTGTGGAGTGGACATAGGGATGGTAAAATTAGGTGTTGGAGGATGGACTCCTCCTCCTCAGCACTAGTAACTGCTCCTTTTAAGGAGGGCTTGTCTTGGCAGGCTCATAGAGGCCCCGTTCTTTCACTTGTTATTTCTTGTTACGGGGATCTCTGGTCAGGTTCAGAGGGTGGTGTCATCAAGATATGGCCATGGGAAGCTATTGAAAAAGCTCTGTCTCTTACCATTGAAGAAAGGCACATGTCTTCTTTGTTGGTGGAGAGGTCTTACATTGATCCCTGGACCCAAGTTGCCGTCAATGGTTTCACTAATGTATTAACTTCAGATGTTAGGTACTTATTATCTGATAGCTCCAGTGCAAAGGTGTGGAGTGCTGGTTATCTCTCATTCGCACTGTGGGATGCGCGCACAAGAGAATTACTGAAAGTGTTCAATACCGATGGTCTGAATGAGAATCGAGTGGACATTTCATCAGCACAGGACTTGTCAGTGGAATTTATTTCTGGAGCAAAAAAAGACAAAACACAAAGTTCTTTTGGTTTCTTTCAGCGATCACGTAATGCTTTAATGGGAGCAGCAGATGCTGTTCGTCGAGTTGCAGTGAAGGGTGCCTTTGGAGATGATAATCGAAGAACTGAAGCATTGGTAATTGCAGTAGATACAATGATTTGGACGGGGTGTGCAAATGGCCTACTTGTGCAGTGGGATAGAAATGGCAACCGTATGCAAGAATTCCATTACCATTCTTCTGCTGTCCAATGCTTTTGTACTTTTGGGCTACGTATATGGGTGGGTTATGCAAGTGGCACTGTCCAGGTATTGGACCTCAACGGTAATCTGCTTGGAGGATGGGTAGCTGACAACAGTCCTATTATTAAAATAGCTACAGGAGCTGGTTATGTATTTACCTTGGCCAATCATGGTGGTATATGTGGATGGAATATTACATCACCGGGACCTCTTGACAGCATAGTGCGTTCAGAATTAGCTGGCAAAGAATTTCTATATACAAGGATAGAAAACTTGAAAATATTGACTGGTACATGGAACGTTGGACAAGGACGAGCGTCTCAGGACTCACTTATATCCTGGCTGGGCTCTGTGGCTTCTGATGTTGGCATTGTTGTTGTTGGTTTGCAAGAAGTTGAAATGGGTGCTGGTTTTCTTGCTATGTCTGCAGCCAAAGAAACTGTAGGTCTTGAGGGGAGCTCTGTTGGGCAGTGGTGGCTGGATATGATTGGAAAAGCATTGGATGAAGGGTCAACCTTTGAACGTGTTGGATCCAGACAGTTGGCAGGATTGCTCATTGCTATGTGGGTGAGACATAATCTCAAAGCTCATGTTGGAGATGTCGATGCTGCAGCAGTTCCGTGTGGTTTTGGGCGCGCTATTGGTAACAAGGGAGCTGTTGGTTTGAGGATTAGATTGTATGACAGGATAATGTGCTTTGTTAATTGTCACTTTGCTGCGCATTTGGAAGCTGTTAATCGCCGCAATGCAGATTTCGATCATGTATATCGAACCATGACCTTCTCCCGACCTAATTTTCTCAATTGTGCAGCTGCTAGTGCTTCATCTGCTGTTCAAATGCTTCGCGGTACAAATGCTATTGGTAACAATTCTGTAGAAGGGATGCCGGAGTTATCTGAAGCAGACATGGTCATATTTCTTGGTGATTTTAACTATCGGCTTGATGGTATATCTTATGATGAAGCTAGAGATTTTGTTTCTCAAAGATGCTTTGATTGGCTTAGAGAACGGGATCAGCTCCGAGTGGAAATGGCAGCTGGAAATGTCTTTCAAGGAATGCGGGAGGCAGATATTACATTTCCTCCCACATACAAGTTTGAAAGGCATCAAGCTGGTTTAGCAGGTGCGTTTAAACGAGTACGGCATACCCAGAATGCCGGCGCCGATGATGGCGATGAAGACATTGGCGAAGGTCTTGGCTTGGGACGACAGCGGCTTCGGGACTCCAATCAGCAGCGTCTTCTAGGTCGCCGGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

879

Amino Acids

96.26

Weight (kDa)

5.87

Isoelectric Point (pI)

37.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_IP5PC_F PF23754 135 - 523 1.3e-187 IP5P C-F beta-propeller
Exo_endo_phos2 PF22669 557 - 830 3.8e-49 Endonuclease/Exonuclease/phosphatase family 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010730)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65580 AT1G65580
fragaria_vesca FvH4_6g00030 FvH4_6g00030
malus_domestica MD12G1260800.v1.1
prunus_persica Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1
pyrus_communis pycom12g24280
rosa_chinensis RchiOBHm_Chr3g0447351
rosa_laevigata RLG00000026007
rosa_multiflora Rmu_ssc0000473.1_g000002
rosa_roxburghii Rroxscaffold_6G00425530
rosa_rugosa Rorug02G0600200 Rorug02G0600300
rosa_samantha Rh3AG000500 Rh3BG000300 Rh3CG000300 Rh3DG000500
rosa_wichuraiana Rw3G000030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2492
Acc16I TGCGCA 1 cut(s) 2100
Acc36I ACCTGC 1 cut(s) 2492
Acc65I GGTACC 1 cut(s) 323
AccB1I GGYRCC 4 cut(s) 323, 562, 1238, 2540
AccB7I CCANNNNNTGG 1 cut(s) 1450
AccI GTMKAC 1 cut(s) 234
AccII CGCG 5 cut(s) 53, 593, 1036, 2016, 2226
AclI AACGTT 1 cut(s) 1680
AclWI GGATC 5 cut(s) 786, 899, 1905, 1918, 2400
AcoI YGGCCR 3 cut(s) 371, 818, 1542
AcsI RAATTY 4 cut(s) 496, 1124, 1361, 1628
AcuI CTGAAG 3 cut(s) 936, 1287, 2298
AcyI GRCGYC 3 cut(s) 144, 264, 2541
AfaI GTAC 7 cut(s) 325, 512, 965, 1399, 1672, 2230, 2520
AfiI CCNNNNNNNGG 5 cut(s) 349, 350, 462, 463, 1450
AflIII ACRYGT 2 cut(s) 870, 1903
AhlI ACTAGT 1 cut(s) 694
AjnI CCWGG 3 cut(s) 908, 1443, 1718
Alw21I GWGCWC 2 cut(s) 587, 1841
Alw26I GTCTC 6 cut(s) 33, 89, 404, 852, 1704, 1945
Alw44I GTGCAC 1 cut(s) 583
AlwI GGATC 5 cut(s) 786, 899, 1905, 1918, 2400
AlwNI CAGNNNCTG 2 cut(s) 1214, 1523
AoxI GGCC 7 cut(s) 309, 371, 392, 742, 818, 1318, 1542
ApaLI GTGCAC 1 cut(s) 583
ApeKI GCWGC 9 cut(s) 1205, 1808, 1988, 1991, 2096, 2189, 2192, 2414, 2614
ApoI RAATTY 4 cut(s) 496, 1124, 1361, 1628
ArsI GACNNNNNNTTYG 4 cut(s) 67, 99, 2580, 2612
Asp700I GAANNNNTTC 1 cut(s) 1058
Asp718I GGTACC 1 cut(s) 323
AspLEI GCGC 8 cut(s) 55, 482, 1036, 1038, 2016, 2018, 2101, 2543
AspS9I GGNCC 6 cut(s) 358, 393, 743, 912, 1453, 1586
AsuC2I CCSGG 2 cut(s) 344, 1584
AsuHPI GGTGA 5 cut(s) 108, 607, 1572, 1960, 2315
AvaII GGWCC 4 cut(s) 358, 912, 1453, 1586
BaeGI GKGCMC 1 cut(s) 587
BalI TGGCCA 2 cut(s) 820, 1544
BamHI GGATCC 1 cut(s) 1910
BanI GGYRCC 4 cut(s) 323, 562, 1238, 2540
BanII GRGCYC 2 cut(s) 1730, 1841
BbsI GAAGAC 3 cut(s) 867, 2565, 2612
Bbv12I GWGCWC 2 cut(s) 587, 1841
BbvCI CCTCAGC 3 cut(s) 404, 528, 688
BbvI GCAGC 9 cut(s) 1217, 1820, 1975, 2003, 2083, 2179, 2201, 2426, 2626
BceAI ACGGC 6 cut(s) 106, 298, 467, 576, 908, 2537
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 3 cut(s) 910, 1445, 1720
BcnI CCSGG 2 cut(s) 344, 1584
BcoDI GTCTC 6 cut(s) 33, 89, 404, 852, 1704, 1945
BcuI ACTAGT 1 cut(s) 694
BfaI CTAG 4 cut(s) 695, 2196, 2346, 2624
BfmI CTRYAG 5 cut(s) 1515, 1806, 1821, 1989, 2253
BfoI RGCGCY 1 cut(s) 2544
BfuAI ACCTGC 1 cut(s) 2492
BglI GCCNNNNNGGC 1 cut(s) 379
Bme1390I CCNGG 5 cut(s) 344, 910, 1445, 1584, 1720
Bme18I GGWCC 4 cut(s) 358, 912, 1453, 1586
BmgT120I GGNCC 6 cut(s) 358, 393, 743, 912, 1453, 1586
BmrFI CCNGG 5 cut(s) 344, 910, 1445, 1584, 1720
BmsI GCATC 7 cut(s) 413, 1021, 1201, 1975, 2253, 2357, 2494
BoxI GACNNNNGTC 3 cut(s) 440, 1494, 1696
BpiI GAAGAC 3 cut(s) 867, 2565, 2612
BplI GAGNNNNNCTC 4 cut(s) 397, 429, 1823, 1855
BpmI CTGGAG 2 cut(s) 967, 1155
Bpu10I CCTNAGC 3 cut(s) 404, 528, 688
BpuEI CTTGAG 1 cut(s) 1850
BpuMI CCSGG 2 cut(s) 344, 1584
BsaAI YACGTR 2 cut(s) 1187, 1412
BsaBI GATNNNNATC 2 cut(s) 1254, 2136
BsaHI GRCGYC 3 cut(s) 144, 264, 2541
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 6 cut(s) 312, 377, 591, 821, 908, 1539
BsaWI WCCGGW 1 cut(s) 326
Bsc4I CCNNNNNNNGG 5 cut(s) 349, 350, 462, 463, 1450
Bse118I RCCGGY 4 cut(s) 369, 517, 2537, 2631
Bse1I ACTGG 2 cut(s) 984, 1672
Bse3DI GCAATG 2 cut(s) 1935, 2131
Bse8I GATNNNNATC 2 cut(s) 1254, 2136
BseBI CCWGG 3 cut(s) 910, 1445, 1720
BseDI CCNNGG 6 cut(s) 312, 377, 591, 821, 908, 1539
BseJI GATNNNNATC 2 cut(s) 1254, 2136
BseLI CCNNNNNNNGG 5 cut(s) 349, 350, 462, 463, 1450
BseMI GCAATG 2 cut(s) 1935, 2131
BseMII CTCAG 8 cut(s) 23, 291, 380, 395, 428, 519, 702, 1715
BseNI ACTGG 2 cut(s) 984, 1672
BsePI GCGCGC 2 cut(s) 1034, 2014
BseRI GAGGAG 6 cut(s) 37, 277, 280, 670, 673, 676
BseSI GKGCMC 1 cut(s) 587
BseX3I CGGCCG 1 cut(s) 371
BseXI GCAGC 9 cut(s) 1217, 1820, 1975, 2003, 2083, 2179, 2201, 2426, 2626
BseYI CCCAGC 2 cut(s) 467, 1723
BsgI GTGCAG 2 cut(s) 1349, 2208
Bsh1236I CGCG 5 cut(s) 53, 593, 1036, 2016, 2226
Bsh1285I CGRYCG 2 cut(s) 202, 374
BshFI GGCC 7 cut(s) 311, 373, 394, 744, 820, 1320, 1544
BshNI GGYRCC 4 cut(s) 323, 562, 1238, 2540
BsiEI CGRYCG 2 cut(s) 202, 374
BsiHKAI GWGCWC 2 cut(s) 587, 1841
BsiSI CCGG 8 cut(s) 327, 344, 370, 518, 1583, 2267, 2538, 2632
BslFI GGGAC 6 cut(s) 326, 439, 484, 1599, 2598, 2615
BslI CCNNNNNNNGG 5 cut(s) 349, 350, 462, 463, 1450
BsmAI GTCTC 6 cut(s) 33, 89, 404, 852, 1704, 1945
BsmBI CGTCTC 2 cut(s) 33, 1704
BsmFI GGGAC 6 cut(s) 326, 439, 484, 1599, 2598, 2615
BsmI GAATGC 2 cut(s) 2442, 2539
BsnI GGCC 7 cut(s) 311, 373, 394, 744, 820, 1320, 1544
Bso31I GGTCTC 1 cut(s) 89
Bsp1286I GDGCHC 3 cut(s) 587, 1730, 1841
Bsp143I GATC 6 cut(s) 778, 904, 1181, 1910, 2137, 2392
Bsp19I CCATGG 1 cut(s) 821
BspANI GGCC 7 cut(s) 311, 373, 394, 744, 820, 1320, 1544
BspCNI CTCAG 8 cut(s) 24, 292, 381, 396, 427, 520, 701, 1714
BspFNI CGCG 5 cut(s) 53, 593, 1036, 2016, 2226
BspMAI CTGCAG 2 cut(s) 1810, 1993
BspMI ACCTGC 1 cut(s) 2492
BspPI GGATC 5 cut(s) 786, 899, 1905, 1918, 2400
BspT107I GGYRCC 4 cut(s) 323, 562, 1238, 2540
BspTNI GGTCTC 1 cut(s) 89
BsrDI GCAATG 2 cut(s) 1935, 2131
BsrFI RCCGGY 4 cut(s) 369, 517, 2537, 2631
BsrI ACTGG 2 cut(s) 984, 1672
BssAI RCCGGY 4 cut(s) 369, 517, 2537, 2631
BssECI CCNNGG 6 cut(s) 312, 377, 591, 821, 908, 1539
BssHII GCGCGC 2 cut(s) 1034, 2014
BssMI GATC 6 cut(s) 778, 904, 1181, 1910, 2137, 2392
BssNI GRCGYC 3 cut(s) 144, 264, 2541
BssT1I CCWWGG 4 cut(s) 312, 377, 821, 1539
Bst2UI CCWGG 3 cut(s) 910, 1445, 1720
Bst4CI ACNGT 9 cut(s) 17, 567, 1026, 1352, 1441, 1463, 1496, 1822, 1920
Bst6I CTCTTC 2 cut(s) 107, 118
BstACI GRCGYC 3 cut(s) 144, 264, 2541
BstAPI GCANNNNNTGC 1 cut(s) 1211
BstBAI YACGTR 2 cut(s) 1187, 1412
BstC8I GCNNGC 8 cut(s) 206, 519, 732, 1036, 1621, 2016, 2539, 2633
BstDEI CTNAG 9 cut(s) 32, 300, 389, 404, 414, 528, 688, 1701, 2381
BstDSI CCRYGG 2 cut(s) 591, 821
BstFNI CGCG 5 cut(s) 53, 593, 1036, 2016, 2226
BstH2I RGCGCY 1 cut(s) 2544
BstHHI GCGC 8 cut(s) 55, 482, 1036, 1038, 2016, 2018, 2101, 2543
BstKTI GATC 6 cut(s) 781, 907, 1184, 1913, 2140, 2395
BstMAI GTCTC 6 cut(s) 33, 89, 404, 852, 1704, 1945
BstMBI GATC 6 cut(s) 778, 904, 1181, 1910, 2137, 2392
BstMCI CGRYCG 2 cut(s) 202, 374
BstNI CCWGG 3 cut(s) 910, 1445, 1720
BstNSI RCATGY 1 cut(s) 874
BstPAI GACNNNNGTC 3 cut(s) 440, 1494, 1696
BstSCI CCNGG 5 cut(s) 342, 908, 1443, 1582, 1718
BstSFI CTRYAG 5 cut(s) 1515, 1806, 1821, 1989, 2253
BstSLI GKGCMC 1 cut(s) 587
BstSNI TACGTA 1 cut(s) 1412
BstUI CGCG 5 cut(s) 53, 593, 1036, 2016, 2226
BstV1I GCAGC 9 cut(s) 1217, 1820, 1975, 2003, 2083, 2179, 2201, 2426, 2626
BstV2I GAAGAC 3 cut(s) 867, 2565, 2612
BstX2I RGATCY 2 cut(s) 778, 1910
BstXI CCANNNNNNTGG 2 cut(s) 80, 1921
BstYI RGATCY 2 cut(s) 778, 1910
BstZI CGGCCG 1 cut(s) 371
BsuRI GGCC 7 cut(s) 311, 373, 394, 744, 820, 1320, 1544
BtgI CCRYGG 2 cut(s) 591, 821
BtgZI GCGATG 1 cut(s) 2568
BtsI GCAGTG 4 cut(s) 273, 1236, 1337, 1856
BtsIMutI CAGTG 8 cut(s) 273, 991, 1022, 1125, 1236, 1337, 1437, 1856
BveI ACCTGC 1 cut(s) 2492
Cac8I GCNNGC 8 cut(s) 206, 519, 732, 1036, 1621, 2016, 2539, 2633
CaiI CAGNNNCTG 2 cut(s) 1214, 1523
CfoI GCGC 8 cut(s) 55, 482, 1036, 1038, 2016, 2018, 2101, 2543
Cfr10I RCCGGY 4 cut(s) 369, 517, 2537, 2631
Cfr13I GGNCC 6 cut(s) 358, 393, 743, 912, 1453, 1586
Cfr42I CCGCGG 1 cut(s) 594
CseI GACGC 4 cut(s) 152, 272, 1686, 2606
Csp6I GTAC 7 cut(s) 324, 511, 964, 1398, 1671, 2229, 2519
CspCI CAANNNNNGTGG 2 cut(s) 1534, 1569
CviAII CATG 8 cut(s) 822, 871, 1550, 1674, 1970, 2141, 2155, 2287
CviQI GTAC 7 cut(s) 324, 511, 964, 1398, 1671, 2229, 2519
DdeI CTNAG 9 cut(s) 32, 300, 389, 404, 414, 528, 688, 1701, 2381
DinI GGCGCC 1 cut(s) 2542
DpnI GATC 6 cut(s) 780, 906, 1183, 1912, 2139, 2394
DpnII GATC 6 cut(s) 778, 904, 1181, 1910, 2137, 2392
DraI TTTAAA 2 cut(s) 502, 2512
EaeI YGGCCR 3 cut(s) 371, 818, 1542
EagI CGGCCG 1 cut(s) 371
Eam1104I CTCTTC 2 cut(s) 107, 118
EarI CTCTTC 2 cut(s) 107, 118
EciI GGCGGA 1 cut(s) 333
Ecl136II GAGCTC 1 cut(s) 1839
EclXI CGGCCG 1 cut(s) 371
Eco105I TACGTA 1 cut(s) 1412
Eco130I CCWWGG 4 cut(s) 312, 377, 821, 1539
Eco24I GRGCYC 2 cut(s) 1730, 1841
Eco31I GGTCTC 1 cut(s) 89
Eco47I GGWCC 4 cut(s) 358, 912, 1453, 1586
Eco52I CGGCCG 1 cut(s) 371
Eco53kI GAGCTC 1 cut(s) 1839
Eco57I CTGAAG 3 cut(s) 936, 1287, 2298
EcoICRI GAGCTC 1 cut(s) 1839
EcoO109I RGGNCCY 3 cut(s) 393, 743, 1586
EcoRI GAATTC 1 cut(s) 1361
EcoRII CCWGG 3 cut(s) 908, 1443, 1718
EcoT14I CCWWGG 4 cut(s) 312, 377, 821, 1539
EcoT38I GRGCYC 2 cut(s) 1730, 1841
EgeI GGCGCC 1 cut(s) 2542
EheI GGCGCC 1 cut(s) 2542
ErhI CCWWGG 4 cut(s) 312, 377, 821, 1539
Esp3I CGTCTC 2 cut(s) 33, 1704
FaeI CATG 8 cut(s) 825, 874, 1553, 1677, 1973, 2144, 2158, 2290
FalI AAGNNNNNCTT 4 cut(s) 704, 736, 1226, 1258
FaqI GGGAC 6 cut(s) 326, 439, 484, 1599, 2598, 2615
FatI CATG 8 cut(s) 821, 870, 1549, 1673, 1969, 2140, 2154, 2286
FauI CCCGC 1 cut(s) 2433
FblI GTMKAC 1 cut(s) 234
FriOI GRGCYC 2 cut(s) 1730, 1841
FspBI CTAG 4 cut(s) 695, 2196, 2346, 2624
FspI TGCGCA 1 cut(s) 2100
GlaI GCGC 8 cut(s) 54, 481, 1035, 1037, 2015, 2017, 2100, 2542
GsaI CCCAGC 2 cut(s) 471, 1727
GsuI CTGGAG 2 cut(s) 967, 1155
HaeII RGCGCY 1 cut(s) 2544
HaeIII GGCC 7 cut(s) 311, 373, 394, 744, 820, 1320, 1544
HapII CCGG 8 cut(s) 327, 344, 370, 518, 1583, 2267, 2538, 2632
HgaI GACGC 4 cut(s) 152, 272, 1686, 2606
HhaI GCGC 8 cut(s) 55, 482, 1036, 1038, 2016, 2018, 2101, 2543
Hin1I GRCGYC 3 cut(s) 144, 264, 2541
Hin1II CATG 8 cut(s) 825, 874, 1553, 1677, 1973, 2144, 2158, 2290
Hin6I GCGC 8 cut(s) 53, 480, 1034, 1036, 2014, 2016, 2099, 2541
HinP1I GCGC 8 cut(s) 53, 480, 1034, 1036, 2014, 2016, 2099, 2541
HincII GTYRAC 3 cut(s) 226, 235, 1893
HindII GTYRAC 3 cut(s) 226, 235, 1893
HinfI GANTC 9 cut(s) 110, 412, 452, 551, 575, 677, 1084, 1706, 2603
HpaII CCGG 8 cut(s) 327, 344, 370, 518, 1583, 2267, 2538, 2632
HphI GGTGA 5 cut(s) 108, 607, 1572, 1960, 2315
Hpy166II GTNNAC 8 cut(s) 109, 226, 235, 361, 585, 641, 1093, 1893
Hpy188III TCNNGA 9 cut(s) 86, 811, 1134, 1592, 1703, 1829, 1914, 2166, 2600
Hpy8I GTNNAC 8 cut(s) 109, 226, 235, 361, 585, 641, 1093, 1893
Hpy99I CGWCG 6 cut(s) 17, 92, 233, 236, 266, 1224
HpyAV CCTTC 6 cut(s) 539, 1228, 1880, 2170, 2252, 2564
HpyCH4III ACNGT 9 cut(s) 17, 567, 1026, 1352, 1441, 1463, 1496, 1822, 1920
HpyCH4IV ACGT 6 cut(s) 222, 228, 1186, 1411, 1680, 1903
HpyF3I CTNAG 9 cut(s) 32, 300, 389, 404, 414, 528, 688, 1701, 2381
HpySE526I ACGT 6 cut(s) 222, 228, 1186, 1411, 1680, 1903
Hsp92I GRCGYC 3 cut(s) 144, 264, 2541
Hsp92II CATG 8 cut(s) 825, 874, 1553, 1677, 1973, 2144, 2158, 2290
HspAI GCGC 8 cut(s) 53, 480, 1034, 1036, 2014, 2016, 2099, 2541
KasI GGCGCC 1 cut(s) 2540
KpnI GGTACC 1 cut(s) 327
KroI GCCGGC 3 cut(s) 517, 2537, 2631
KroNI GCCGGC 3 cut(s) 519, 2539, 2633
KspI CCGCGG 1 cut(s) 594
Kzo9I GATC 6 cut(s) 778, 904, 1181, 1910, 2137, 2392
Lsp1109I GCAGC 9 cut(s) 1217, 1820, 1975, 2003, 2083, 2179, 2201, 2426, 2626
LweI GCATC 7 cut(s) 413, 1021, 1201, 1975, 2253, 2357, 2494
MaeI CTAG 4 cut(s) 695, 2196, 2346, 2624
MaeII ACGT 6 cut(s) 222, 228, 1186, 1411, 1680, 1903
MaeIII GTNAC 6 cut(s) 41, 697, 770, 2024, 2087, 2243
MalI GATC 6 cut(s) 780, 906, 1183, 1912, 2139, 2394
MboI GATC 6 cut(s) 778, 904, 1181, 1910, 2137, 2392
MfeI CAATTG 2 cut(s) 458, 2182
MflI RGATCY 2 cut(s) 778, 1910
MhlI GDGCHC 3 cut(s) 587, 1730, 1841
MlsI TGGCCA 2 cut(s) 820, 1544
MluNI TGGCCA 2 cut(s) 820, 1544
Mly113I GGCGCC 1 cut(s) 2541
MlyI GAGTC 7 cut(s) 104, 406, 461, 584, 671, 1700, 2597
MmeI TCCRAC 4 cut(s) 647, 1663, 1888, 1954
Mox20I TGGCCA 2 cut(s) 820, 1544
MreI CGCCGGCG 2 cut(s) 517, 2631
MroNI GCCGGC 3 cut(s) 517, 2537, 2631
MroXI GAANNNNTTC 1 cut(s) 1058
MscI TGGCCA 2 cut(s) 820, 1544
MseI TTAA 9 cut(s) 501, 711, 947, 1196, 1506, 2082, 2115, 2310, 2511
MslI CAYNNNNRTG 2 cut(s) 648, 1940
Msp20I TGGCCA 2 cut(s) 820, 1544
MspA1I CMGCKG 5 cut(s) 403, 593, 2192, 2417, 2593
MspI CCGG 8 cut(s) 327, 344, 370, 518, 1583, 2267, 2538, 2632
MspR9I CCNGG 5 cut(s) 344, 910, 1445, 1584, 1720
MssI GTTTAAAC 1 cut(s) 2512
MunI CAATTG 2 cut(s) 458, 2182
Mva1269I GAATGC 2 cut(s) 2442, 2539
MvaI CCWGG 3 cut(s) 910, 1445, 1720
MvnI CGCG 5 cut(s) 53, 593, 1036, 2016, 2226
NaeI GCCGGC 3 cut(s) 519, 2539, 2633
NarI GGCGCC 1 cut(s) 2541
NciI CCSGG 2 cut(s) 344, 1584
NcoI CCATGG 1 cut(s) 821
NdeII GATC 6 cut(s) 778, 904, 1181, 1910, 2137, 2392
NgoMIV GCCGGC 3 cut(s) 517, 2537, 2631
NlaIII CATG 8 cut(s) 825, 874, 1553, 1677, 1973, 2144, 2158, 2290
NmuCI GTSAC 1 cut(s) 2087
NsbI TGCGCA 1 cut(s) 2100
NspI RCATGY 1 cut(s) 874
PaqCI CACCTGC 1 cut(s) 2492
PauI GCGCGC 2 cut(s) 1034, 2014
PciI ACATGT 1 cut(s) 870
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 228
PctI GAATGC 2 cut(s) 2442, 2539
PdiI GCCGGC 3 cut(s) 519, 2539, 2633
PdmI GAANNNNTTC 1 cut(s) 1058
PfeI GAWTC 2 cut(s) 551, 1084
PflFI GACNNNGTC 1 cut(s) 2288
PflMI CCANNNNNTGG 1 cut(s) 1450
PleI GAGTC 7 cut(s) 104, 406, 460, 583, 671, 1700, 2597
PluTI GGCGCC 1 cut(s) 2544
PmeI GTTTAAAC 1 cut(s) 2512
PpsI GAGTC 7 cut(s) 104, 406, 460, 583, 671, 1700, 2597
Ppu21I YACGTR 2 cut(s) 1187, 1412
PpuMI RGGWCCY 1 cut(s) 1586
PscI ACATGT 1 cut(s) 870
PshAI GACNNNNGTC 3 cut(s) 440, 1494, 1696
Psp124BI GAGCTC 1 cut(s) 1841
Psp1406I AACGTT 1 cut(s) 1680
Psp5II RGGWCCY 1 cut(s) 1586
Psp6I CCWGG 3 cut(s) 908, 1443, 1718
PspFI CCCAGC 2 cut(s) 467, 1723
PspGI CCWGG 3 cut(s) 908, 1443, 1718
PspPI GGNCC 6 cut(s) 358, 393, 743, 912, 1453, 1586
PspPPI RGGWCCY 1 cut(s) 1586
PstI CTGCAG 2 cut(s) 1810, 1993
PstNI CAGNNNCTG 2 cut(s) 1214, 1523
PsuI RGATCY 2 cut(s) 778, 1910
PsyI GACNNNGTC 1 cut(s) 2288
PteI GCGCGC 2 cut(s) 1034, 2014
PvuII CAGCTG 2 cut(s) 2192, 2417
RsaI GTAC 7 cut(s) 325, 512, 965, 1399, 1672, 2230, 2520
RsaNI GTAC 7 cut(s) 324, 511, 964, 1398, 1671, 2229, 2519
RseI CAYNNNNRTG 2 cut(s) 648, 1940
SacI GAGCTC 1 cut(s) 1841
SacII CCGCGG 1 cut(s) 594
SalI GTCGAC 1 cut(s) 233
SaqAI TTAA 9 cut(s) 501, 711, 947, 1196, 1506, 2082, 2115, 2310, 2511
Sau3AI GATC 6 cut(s) 778, 904, 1181, 1910, 2137, 2392
Sau96I GGNCC 6 cut(s) 358, 393, 743, 912, 1453, 1586
SchI GAGTC 7 cut(s) 104, 406, 461, 584, 671, 1700, 2597
ScrFI CCNGG 5 cut(s) 344, 910, 1445, 1584, 1720
SduI GDGCHC 3 cut(s) 587, 1730, 1841
SfaNI GCATC 7 cut(s) 413, 1021, 1201, 1975, 2253, 2357, 2494
SfcI CTRYAG 5 cut(s) 1515, 1806, 1821, 1989, 2253
SfoI GGCGCC 1 cut(s) 2542
Sfr303I CCGCGG 1 cut(s) 594
SgrAI CRCCGGYG 2 cut(s) 517, 2631
SgrBI CCGCGG 1 cut(s) 594
SinI GGWCC 4 cut(s) 358, 912, 1453, 1586
SmiMI CAYNNNNRTG 2 cut(s) 648, 1940
SmlI CTYRAG 1 cut(s) 1829
SmoI CTYRAG 1 cut(s) 1829
SnaBI TACGTA 1 cut(s) 1412
SpeI ACTAGT 1 cut(s) 694
SspDI GGCGCC 1 cut(s) 2540
SspI AATATT 2 cut(s) 1573, 1662
SspMI CTAG 4 cut(s) 695, 2196, 2346, 2624
SstI GAGCTC 1 cut(s) 1841
StyD4I CCNGG 5 cut(s) 342, 908, 1443, 1582, 1718
StyI CCWWGG 4 cut(s) 312, 377, 821, 1539
TaaI ACNGT 9 cut(s) 17, 567, 1026, 1352, 1441, 1463, 1496, 1822, 1920
TaiI ACGT 6 cut(s) 225, 231, 1189, 1414, 1683, 1906
TatI WGTACW 2 cut(s) 510, 1397
TauI GCSGC 8 cut(s) 96, 149, 269, 376, 524, 527, 2124, 2596
TfiI GAWTC 2 cut(s) 551, 1084
Tru1I TTAA 9 cut(s) 501, 711, 947, 1196, 1506, 2082, 2115, 2310, 2511
Tru9I TTAA 9 cut(s) 501, 711, 947, 1196, 1506, 2082, 2115, 2310, 2511
TscAI CASTG 8 cut(s) 280, 991, 1029, 1125, 1236, 1337, 1444, 1856
TseFI GTSAC 1 cut(s) 2087
TseI GCWGC 9 cut(s) 1205, 1808, 1988, 1991, 2096, 2189, 2192, 2414, 2614
Tsp45I GTSAC 1 cut(s) 2087
TspDTI ATGAA 7 cut(s) 114, 150, 1089, 1899, 2193, 2355, 2571
TspGWI ACGGA 5 cut(s) 29, 202, 488, 568, 1989
TspRI CASTG 8 cut(s) 280, 991, 1029, 1125, 1236, 1337, 1444, 1856
Tth111I GACNNNGTC 1 cut(s) 2288
Van91I CCANNNNNTGG 1 cut(s) 1450
VneI GTGCAC 1 cut(s) 583
VpaK11BI GGWCC 4 cut(s) 358, 912, 1453, 1586
XapI RAATTY 4 cut(s) 496, 1124, 1361, 1628
XceI RCATGY 1 cut(s) 874
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 234
XmnI GAANNNNTTC 1 cut(s) 1058
XspI CTAG 4 cut(s) 695, 2196, 2346, 2624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.