MD12G1260800.v1.1

Type II inositol 1,4,5-trisphosphate 5-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010730
Physical Location & Seq
Forward (+)
32708099 .. 32717394
9296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1260800.v1.1.491

Sequence Viewer

Length: 3369 bp
ATGGATGAAAACGAGGACCTGTTTTCGTCCCCGAGCTTCTCCGGTAACCCACCACCAAGTGACCTCCGCAAATTCAGCCTCCTCGACCAGACTCGCGCTGACACACTTACTCCTTCCGCTTCAAAGCTATTCCCGGACTCTTCCTCTTCCTCATCGGAAGATGAGGACAACGTCGTCTCCCTCCACTCCACCTCCAAACGCCTCGACTACATGCTCCAATTCCTTGACCGCAAGCTCTCCGTCATCAACGACGACGACTACAACAAGAACAACAACAGCAGCAGTAATTATAACAATAATAATAATAAGAACAACGCCTCCGAGGGGAACTGCTCGTCTTCGCTTCCGGAGTTCTTGGCCAGTGGGGGAGGGACTGGGATCTTCAAAGTCCCGATTCGGGCGGCTGTCCATCCGAACCGGCCGCCGAGGCTGGAAGTGAGGCCCCACCCGCTGAGGGAGACTCAGATAGGGTGCTTCTTGCGGACAATGGTGAGCACCGCGTCCCAATTGTGGGTTGGGACGGAGTGCGCCGTTAGGGTTTGGAACTTGAGCGATCTGTACTCGGCGGCAGGACAAGGCGAATCAGGGGATGAGGAGGCGGTGCCGTATAGGGAGTCGGTATGCACATCGGCTGTGATATGCTTGGTTGGGGATGAGGGGAATAAGATGGTGTGGAGCGGGCATAGGGATGGAAGGATCAGGTGTTGGAAGATGGATTCGGCTCCTACTCCGACGAATCCGTTTAAGGAGGGCTTATCTTGGCAGGCTCACAGAGGCCCCGTTCTTTCAATTGTCATCTCCTGCTATGGGGATCTATGGTCAGGCTCAGAGGGTGGTTCCATCAAGATATGGCCACGGGAAGCTCTTGAAAAAGCTCTCTCCCTAACAGCCGAAGAAAGGCACATGTCTTCTTTGTTGGTCGAGAGATCTTACATTGAACCTTGGACCCAAGTTGCCGTCAATGGTTTCACTAACATATTAACTTCAGATGTTCGCTACTTATTATCTGATCGTTCTGGAGCCAAGCTCTGGACTGCTGGTTATCTCTCATTCGCATTGTGGGATGCTCGTACAAGGGAGTTATTGAAGCTTTTCAGTACAGATGGTCAGATTGAGAATCGAGTGGACATTTCATCAGCACAGGACTTCTCAGCTGAACCTATTGCTGGATCAAAGAAAGACAAAATACAAAGTTCTTTTGGTTTCTTTCAGCGGTCACGTAATGCCATAATGGGAGCAGCAGATGCTGTTCGTCGAGTTGCAGTGAAGGGTGCCTTTGGAGATGATAATCGAAGAACTGAAGCACTGGTGATAGCAATGGATGGAATGATTTGGACTGGGTGTACAAATGGCCTACTTGTACAATGGGATAGAAATGGCAATCGTATACAAGAATACCACTATCATTCTTCTGCTGTCCAATGCTTTTGTACATTTGGGCTACGTATATGGGTTGGTTATGCAAGTGGTACTGTCCATGTATTGGACCTTGAGGGTAATCTGCTTGGAGGATGGGTGGCTCACAGCAGTCCTGTCATAAAAATGGCTGCAGGAGCGGGTTTTATATTTACCTTGGCTAATCAAGGTGGTATATGTGGATGGAATATCACGTCCCCTGGACCTCTTGACAATATATTGTGCTCAGAATTAGCCGGCAAAGAATTTACATATACAAGGATAGAAAATTTGAAAATATTGACCGGTACATGGAATGTTGGACAGGGAAGAGCATCTCAGGACTCACTTATATCCTGGCTGGGCTCTGTTGCTGCTACTGTTGGAATTGTTGTTGTTGGGTTGCAAGAAGTTGAAATGGGCGCTGGTTTTCTTGCCATGTCTGCAGCCAAAGAAACTGTAGGGCTTGAGGGCAGCTCTGTTGGGCAGTGGTGGCTGGATATGATCGGAAAAACATTGGATGAAGGTTTGACATTTGAGCGCGTTGGCTCCAGGCAATTGGCAGGATTGCTCATTGCTGTGTGGGTGAGAAACAATCTTAGGACTCATGTTGGGGATGTTGATGCTGCAGCAGTTCCATGTGGTTTTGGGCGTGCCATTGGTAACAAGGGAGCTGTTGGTTTGAGGATTAGAATATATGGCCGGGTAATGTGCTTTGTTAATTGTCACTTTGCTGCACACTTAGAAGCCGTTAATCGCCGCAATGGAGACTTTGATCATGTATATCGAACAATGACCTTCTGCCGACCTAACTTTCTCAATTGTGCAGCTGCTAGTGCTTCATCTGCAGTTCAAATGCTTCGTGGCACACATGCTATTGGTAACAATTCTGCAGAAGGGATGCCTGAGTTATCTGAGGCAGACATGATCATATTTCTTGGCGATTTTAACTATCGACTTGATGGTATATCTTACGATGAAGCTAGAGATTTTGTTTCTCAAAGATGTTTTGATTGGCTTAGAGAAAGGGATCAGCTCCGAGTGGAAATGGAAGCTGGAAATGTCTTCCAAGGAATGCGTGAGGCAGATATTAAGTTTCCTCCCACGTATAAGTTTGAAAGGCATCAAGCTGGTTTAGCAGGATATGATTCTGGTGAGAAGAAGCGTACTCCAGCTTGGTGCGATAGAATCTTGTACCGTGACAGCCGCTCAGCGTCAGTATCAGAGTGCAGCTTAGAATGCCCTGTAGTGTCTTCAATCTCACAGTACGAGGCCTGCATGGATGTGACAGACAGTGATCACAAGCCTGTCCGTTGCATTTTTACTGTTGATATTGCTCGAGTTGATGAGTCTCTAAGGAGACAAGAGTTTGGAGAAATCCTTAAATCAAATGAGAAAATCAAATGTATTATCGAAGAACAATGCAAGATTCCAGAAACTATTGTCAGTACAAACAACATAATCCTTCAAAACCAGGACACCTCCATTTTGCGTATTACAAATAAATGTGGCGATAAAGATGCTTTTTTTGATATTATTTGCGAAGGACAGTCAATCATTAAGGAGGATGGACCCACATCAGACTATTGTTCCTTTGGCTTTCCACGATGGCTTGAGGTTACTCCTTCAGCTGGCATTATCAGACCAGATCATATAGCTGAGGTGACAGTTCATCACGAGGAGCACCAAACCCTAGAAGAGTTTCTTGATGGTGTTCCACAAAATTGGTGGTGTGAAGACACCAGAGACAAGGAAGTCATATTGGTGGTTAAGGTGCGTGGCAGTTACACTACTGACACAAGACATCACCGTGTCTGTGTGCGCCAATGCTGTTCAGCCAAGACAAACCAAAATGAACCCACTGGTGACAGCACCAGACAAGCACAAGGGACGGTTCTTCGCCGGTCTGATTTTCAACACCTGAGCAGTTCCTATGATGTGGTTGATCATCTCTGGAGTTCGCGTAGTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

1123

Amino Acids

124.34

Weight (kDa)

5.57

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_IP5PC_F PF23754 151 - 534 2.7e-182 IP5P C-F beta-propeller
Exo_endo_phos2 PF22669 568 - 903 8.3e-65 Endonuclease/Exonuclease/phosphatase family 2
Exo_endo_phos PF03372 568 - 899 1.7e-07 Endonuclease/Exonuclease/phosphatase family
Ig-like_IP5PC_F PF23755 942 - 1075 5.9e-67 IP5P C-F Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010730)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65580 AT1G65580
fragaria_vesca FvH4_6g00030 FvH4_6g00030
malus_domestica MD12G1260800.v1.1
prunus_persica Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1
pyrus_communis pycom12g24280
rosa_chinensis RchiOBHm_Chr3g0447351
rosa_laevigata RLG00000026007
rosa_multiflora Rmu_ssc0000473.1_g000002
rosa_roxburghii Rroxscaffold_6G00425530
rosa_rugosa Rorug02G0600200 Rorug02G0600300
rosa_samantha Rh3AG000500 Rh3BG000300 Rh3CG000300 Rh3DG000500
rosa_wichuraiana Rw3G000030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 291
AasI GACNNNNNNGTC 2 cut(s) 173, 3149
AccB1I GGYRCC 2 cut(s) 601, 1271
AccB7I CCANNNNNTGG 2 cut(s) 1029, 1483
AccBSI CCGCTC 3 cut(s) 678, 1556, 2604
AccI GTMKAC 1 cut(s) 1387
AccII CGCG 4 cut(s) 96, 500, 1938, 3358
AccIII TCCGGA 1 cut(s) 346
AclWI GGATC 5 cut(s) 386, 704, 819, 1177, 2433
AcoI YGGCCR 4 cut(s) 357, 419, 851, 2095
AcsI RAATTY 3 cut(s) 71, 1661, 1684
AcuI CTGAAG 3 cut(s) 969, 1320, 3006
AdeI CACNNNGTG 1 cut(s) 59
AflIII ACRYGT 1 cut(s) 903
AgeI ACCGGT 1 cut(s) 1700
AjiI CACGTC 1 cut(s) 1611
AjnI CCWGG 4 cut(s) 1615, 1751, 1946, 2868
AloI GAACNNNNNNTCC 2 cut(s) 302, 334
Alw21I GWGCWC 3 cut(s) 497, 1643, 3081
Alw26I GTCTC 6 cut(s) 181, 452, 2157, 2749, 2751, 3135
AlwI GGATC 5 cut(s) 386, 704, 819, 1177, 2433
AlwNI CAGNNNCTG 1 cut(s) 1247
Ama87I CYCGRG 2 cut(s) 31, 2733
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 8 cut(s) 357, 419, 440, 775, 851, 1351, 2095, 2667
ApoI RAATTY 3 cut(s) 71, 1661, 1684
AsiGI ACCGGT 1 cut(s) 1700
Asp700I GAANNNNTTC 2 cut(s) 1091, 3096
AspLEI GCGC 5 cut(s) 98, 530, 1820, 1938, 3219
AspS9I GGNCC 7 cut(s) 16, 441, 776, 945, 1486, 1619, 2966
AsuC2I CCSGG 2 cut(s) 134, 2099
AsuHPI GGTGA 7 cut(s) 502, 1321, 1993, 2561, 3070, 3194, 3272
AvaI CYCGRG 2 cut(s) 31, 2733
AvaII GGWCC 5 cut(s) 16, 945, 1486, 1619, 2966
BalI TGGCCA 2 cut(s) 359, 853
BanI GGYRCC 2 cut(s) 601, 1271
BanII GRGCYC 1 cut(s) 1763
BauI CACGAG 1 cut(s) 3071
BbsI GAAGAC 5 cut(s) 330, 900, 2452, 2640, 3138
Bbv12I GWGCWC 3 cut(s) 497, 1643, 3081
BbvCI CCTCAGC 2 cut(s) 452, 3054
BceAI ACGGC 4 cut(s) 515, 589, 941, 2129
BcgI CGANNNNNNTGC 2 cut(s) 2897, 2931
BciT130I CCWGG 4 cut(s) 1617, 1753, 1948, 2870
BclI TGATCA 4 cut(s) 2170, 2322, 2692, 3340
BcnI CCSGG 2 cut(s) 134, 2099
BcoDI GTCTC 6 cut(s) 181, 452, 2157, 2749, 2751, 3135
BfaI CTAG 3 cut(s) 2229, 2379, 3089
BfmI CTRYAG 7 cut(s) 1548, 1839, 1854, 2022, 2241, 2286, 2640
BfoI RGCGCY 1 cut(s) 1821
BglI GCCNNNNNGGC 1 cut(s) 427
BglII AGATCT 1 cut(s) 926
BlpI GCTNAGC 1 cut(s) 2605
Bme1390I CCNGG 6 cut(s) 134, 1617, 1753, 1948, 2099, 2870
Bme18I GGWCC 5 cut(s) 16, 945, 1486, 1619, 2966
BmeT110I CYCGRG 2 cut(s) 31, 2733
BmgBI CACGTC 1 cut(s) 1611
BmgT120I GGNCC 7 cut(s) 16, 441, 776, 945, 1486, 1619, 2966
BmrFI CCNGG 6 cut(s) 134, 1617, 1753, 1948, 2099, 2870
BmrI ACTGGG 2 cut(s) 384, 1347
BmsI GCATC 7 cut(s) 1054, 1234, 1739, 2008, 2286, 2527, 2905
BmuI ACTGGG 2 cut(s) 384, 1347
BpiI GAAGAC 5 cut(s) 330, 900, 2452, 2640, 3138
BplI GAGNNNNNCTC 6 cut(s) 445, 477, 1011, 1043, 1856, 1888
BpmI CTGGAG 3 cut(s) 1038, 1930, 2550
Bpu10I CCTNAGC 3 cut(s) 452, 3054, 3317
Bpu1102I GCTNAGC 1 cut(s) 2605
BpuEI CTTGAG 4 cut(s) 568, 1511, 1883, 3029
BpuMI CCSGG 2 cut(s) 134, 2099
BsaAI YACGTR 3 cut(s) 1220, 1445, 2502
BsaBI GATNNNNATC 1 cut(s) 1287
BsaJI CCNNGG 7 cut(s) 321, 425, 854, 941, 1572, 1615, 2464
BsaWI WCCGGW 3 cut(s) 41, 346, 1700
Bse118I RCCGGY 4 cut(s) 417, 1652, 1700, 3297
Bse1I ACTGG 5 cut(s) 360, 379, 1311, 1342, 3262
Bse3DI GCAATG 3 cut(s) 1323, 1968, 2164
Bse8I GATNNNNATC 1 cut(s) 1287
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 4 cut(s) 1617, 1753, 1948, 2870
BseDI CCNNGG 7 cut(s) 321, 425, 854, 941, 1572, 1615, 2464
BseJI GATNNNNATC 1 cut(s) 1287
BseMI GCAATG 3 cut(s) 1323, 1968, 2164
BseNI ACTGG 5 cut(s) 360, 379, 1311, 1342, 3262
BseRI GAGGAG 3 cut(s) 71, 608, 3089
BseX3I CGGCCG 1 cut(s) 419
BseYI CCCAGC 1 cut(s) 1756
BsgI GTGCAG 3 cut(s) 2115, 2241, 2644
Bsh1236I CGCG 4 cut(s) 96, 500, 1938, 3358
Bsh1285I CGRYCG 1 cut(s) 422
BshFI GGCC 8 cut(s) 359, 421, 442, 777, 853, 1353, 2097, 2669
BshNI GGYRCC 2 cut(s) 601, 1271
BshTI ACCGGT 1 cut(s) 1700
BsiEI CGRYCG 1 cut(s) 422
BsiHKAI GWGCWC 3 cut(s) 497, 1643, 3081
BsiHKCI CYCGRG 2 cut(s) 31, 2733
BsiSI CCGG 8 cut(s) 42, 134, 347, 418, 1653, 1701, 2098, 3298
BslFI GGGAC 7 cut(s) 13, 374, 385, 487, 532, 1597, 3298
BsmAI GTCTC 6 cut(s) 181, 452, 2157, 2749, 2751, 3135
BsmBI CGTCTC 1 cut(s) 181
BsmFI GGGAC 7 cut(s) 13, 374, 385, 487, 532, 1597, 3298
BsmI GAATGC 2 cut(s) 2475, 2639
BsnI GGCC 8 cut(s) 359, 421, 442, 777, 853, 1353, 2097, 2669
BsoBI CYCGRG 2 cut(s) 31, 2733
Bsp1286I GDGCHC 4 cut(s) 497, 1643, 1763, 3081
Bsp13I TCCGGA 1 cut(s) 346
Bsp1407I TGTACA 3 cut(s) 1343, 1360, 1430
Bsp1720I GCTNAGC 1 cut(s) 2605
BspANI GGCC 8 cut(s) 359, 421, 442, 777, 853, 1353, 2097, 2669
BspEI TCCGGA 1 cut(s) 346
BspFNI CGCG 4 cut(s) 96, 500, 1938, 3358
BspMAI CTGCAG 5 cut(s) 1552, 1843, 2026, 2245, 2290
BspPI GGATC 5 cut(s) 386, 704, 819, 1177, 2433
BspQI GCTCTTC 1 cut(s) 1720
BspT107I GGYRCC 2 cut(s) 601, 1271
BsrBI CCGCTC 3 cut(s) 678, 1556, 2604
BsrDI GCAATG 3 cut(s) 1323, 1968, 2164
BsrFI RCCGGY 4 cut(s) 417, 1652, 1700, 3297
BsrGI TGTACA 3 cut(s) 1343, 1360, 1430
BsrI ACTGG 5 cut(s) 360, 379, 1311, 1342, 3262
BssAI RCCGGY 4 cut(s) 417, 1652, 1700, 3297
BssECI CCNNGG 7 cut(s) 321, 425, 854, 941, 1572, 1615, 2464
BssNAI GTATAC 1 cut(s) 1388
BssSI CACGAG 1 cut(s) 3071
BssT1I CCWWGG 3 cut(s) 941, 1572, 2464
Bst1107I GTATAC 1 cut(s) 1388
Bst2BI CACGAG 1 cut(s) 3071
Bst2UI CCWGG 4 cut(s) 1617, 1753, 1948, 2870
Bst6I CTCTTC 4 cut(s) 145, 151, 1720, 3087
BstAPI GCANNNNNTGC 1 cut(s) 1244
BstAUI TGTACA 3 cut(s) 1343, 1360, 1430
BstBAI YACGTR 3 cut(s) 1220, 1445, 2502
BstC8I GCNNGC 7 cut(s) 233, 680, 765, 1654, 2049, 2671, 3028
BstDSI CCRYGG 1 cut(s) 854
BstEII GGTNACC 1 cut(s) 44
BstFNI CGCG 4 cut(s) 96, 500, 1938, 3358
BstH2I RGCGCY 1 cut(s) 1821
BstHHI GCGC 5 cut(s) 98, 530, 1820, 1938, 3219
BstMAI GTCTC 6 cut(s) 181, 452, 2157, 2749, 2751, 3135
BstMCI CGRYCG 1 cut(s) 422
BstNI CCWGG 4 cut(s) 1617, 1753, 1948, 2870
BstNSI RCATGY 3 cut(s) 214, 907, 2270
BstPI GGTNACC 1 cut(s) 44
BstSCI CCNGG 6 cut(s) 132, 1615, 1751, 1946, 2097, 2868
BstSFI CTRYAG 7 cut(s) 1548, 1839, 1854, 2022, 2241, 2286, 2640
BstSNI TACGTA 1 cut(s) 1445
BstUI CGCG 4 cut(s) 96, 500, 1938, 3358
BstV2I GAAGAC 5 cut(s) 330, 900, 2452, 2640, 3138
BstX2I RGATCY 3 cut(s) 378, 811, 926
BstXI CCANNNNNNTGG 2 cut(s) 1954, 3120
BstYI RGATCY 3 cut(s) 378, 811, 926
BstZ17I GTATAC 1 cut(s) 1388
BstZI CGGCCG 1 cut(s) 419
BsuRI GGCC 8 cut(s) 359, 421, 442, 777, 853, 1353, 2097, 2669
BtgI CCRYGG 1 cut(s) 854
BtrI CACGTC 1 cut(s) 1611
BtsI GCAGTG 2 cut(s) 1269, 1889
BtsIMutI CAGTG 6 cut(s) 367, 1269, 1304, 1889, 2695, 3255
Cac8I GCNNGC 7 cut(s) 233, 680, 765, 1654, 2049, 2671, 3028
CaiI CAGNNNCTG 1 cut(s) 1247
CfoI GCGC 5 cut(s) 98, 530, 1820, 1938, 3219
Cfr10I RCCGGY 4 cut(s) 417, 1652, 1700, 3297
Cfr13I GGNCC 7 cut(s) 16, 441, 776, 945, 1486, 1619, 2966
CseI GACGC 2 cut(s) 489, 2598
CspAI ACCGGT 1 cut(s) 1700
CspCI CAANNNNNGTGG 2 cut(s) 3104, 3139
DraIII CACNNNGTG 1 cut(s) 59
DrdI GACNNNNNNGTC 2 cut(s) 173, 3149
DseDI GACNNNNNNGTC 2 cut(s) 173, 3149
EaeI YGGCCR 4 cut(s) 357, 419, 851, 2095
EagI CGGCCG 1 cut(s) 419
Eam1104I CTCTTC 4 cut(s) 145, 151, 1720, 3087
EarI CTCTTC 4 cut(s) 145, 151, 1720, 3087
EclXI CGGCCG 1 cut(s) 419
Eco105I TACGTA 1 cut(s) 1445
Eco130I CCWWGG 3 cut(s) 941, 1572, 2464
Eco147I AGGCCT 1 cut(s) 2669
Eco24I GRGCYC 1 cut(s) 1763
Eco47I GGWCC 5 cut(s) 16, 945, 1486, 1619, 2966
Eco52I CGGCCG 1 cut(s) 419
Eco57I CTGAAG 3 cut(s) 969, 1320, 3006
Eco88I CYCGRG 2 cut(s) 31, 2733
Eco91I GGTNACC 1 cut(s) 44
EcoO109I RGGNCCY 3 cut(s) 16, 441, 776
EcoO65I GGTNACC 1 cut(s) 44
EcoRII CCWGG 4 cut(s) 1615, 1751, 1946, 2868
EcoT14I CCWWGG 3 cut(s) 941, 1572, 2464
EcoT38I GRGCYC 1 cut(s) 1763
ErhI CCWWGG 3 cut(s) 941, 1572, 2464
Esp3I CGTCTC 1 cut(s) 181
FalI AAGNNNNNCTT 6 cut(s) 737, 769, 1259, 1291, 3084, 3116
FaqI GGGAC 7 cut(s) 13, 374, 385, 487, 532, 1597, 3298
FauI CCCGC 3 cut(s) 456, 671, 1549
FbaI TGATCA 4 cut(s) 2170, 2322, 2692, 3340
FblI GTMKAC 1 cut(s) 1387
FriOI GRGCYC 1 cut(s) 1763
FspBI CTAG 3 cut(s) 2229, 2379, 3089
GlaI GCGC 5 cut(s) 97, 529, 1819, 1937, 3218
GsaI CCCAGC 1 cut(s) 1760
GsuI CTGGAG 3 cut(s) 1038, 1930, 2550
HaeII RGCGCY 1 cut(s) 1821
HaeIII GGCC 8 cut(s) 359, 421, 442, 777, 853, 1353, 2097, 2669
HapII CCGG 8 cut(s) 42, 134, 347, 418, 1653, 1701, 2098, 3298
HgaI GACGC 2 cut(s) 489, 2598
HhaI GCGC 5 cut(s) 98, 530, 1820, 1938, 3219
Hin6I GCGC 5 cut(s) 96, 528, 1818, 1936, 3217
HinP1I GCGC 5 cut(s) 96, 528, 1818, 1936, 3217
HindIII AAGCTT 1 cut(s) 1088
HpaII CCGG 8 cut(s) 42, 134, 347, 418, 1653, 1701, 2098, 3298
HphI GGTGA 7 cut(s) 502, 1321, 1993, 2561, 3070, 3194, 3272
Hpy166II GTNNAC 3 cut(s) 1126, 1344, 1388
Hpy8I GTNNAC 3 cut(s) 1126, 1344, 1388
Hpy99I CGWCG 5 cut(s) 176, 254, 257, 736, 1257
HpyAV CCTTC 9 cut(s) 123, 687, 1261, 1913, 2203, 2285, 2870, 2933, 3030
HpyCH4IV ACGT 5 cut(s) 171, 1219, 1444, 1610, 2501
HpySE526I ACGT 5 cut(s) 171, 1219, 1444, 1610, 2501
HspAI GCGC 5 cut(s) 96, 528, 1818, 1936, 3217
Kpn2I TCCGGA 1 cut(s) 346
KroI GCCGGC 1 cut(s) 1652
KroNI GCCGGC 1 cut(s) 1654
Ksp22I TGATCA 4 cut(s) 2170, 2322, 2692, 3340
LguI GCTCTTC 1 cut(s) 1720
LweI GCATC 7 cut(s) 1054, 1234, 1739, 2008, 2286, 2527, 2905
MaeI CTAG 3 cut(s) 2229, 2379, 3089
MaeII ACGT 5 cut(s) 171, 1219, 1444, 1610, 2501
MbiI CCGCTC 3 cut(s) 678, 1556, 2604
MfeI CAATTG 4 cut(s) 506, 789, 1952, 2215
MflI RGATCY 3 cut(s) 378, 811, 926
MhlI GDGCHC 4 cut(s) 497, 1643, 1763, 3081
MlsI TGGCCA 2 cut(s) 359, 853
MluNI TGGCCA 2 cut(s) 359, 853
MlyI GAGTC 7 cut(s) 85, 131, 454, 623, 1733, 1993, 2753
MmeI TCCRAC 4 cut(s) 686, 755, 1696, 1759
Mox20I TGGCCA 2 cut(s) 359, 853
MroI TCCGGA 1 cut(s) 346
MroNI GCCGGC 1 cut(s) 1652
MroXI GAANNNNTTC 2 cut(s) 1091, 3096
MscI TGGCCA 2 cut(s) 359, 853
MslI CAYNNNNRTG 6 cut(s) 687, 1541, 1973, 2678, 3158, 3204
Msp20I TGGCCA 2 cut(s) 359, 853
MspA1I CMGCKG 5 cut(s) 451, 1154, 1213, 2225, 3026
MspI CCGG 8 cut(s) 42, 134, 347, 418, 1653, 1701, 2098, 3298
MspR9I CCNGG 6 cut(s) 134, 1617, 1753, 1948, 2099, 2870
MunI CAATTG 4 cut(s) 506, 789, 1952, 2215
Mva1269I GAATGC 2 cut(s) 2475, 2639
MvaI CCWGG 4 cut(s) 1617, 1753, 1948, 2870
MvnI CGCG 4 cut(s) 96, 500, 1938, 3358
NaeI GCCGGC 1 cut(s) 1654
NciI CCSGG 2 cut(s) 134, 2099
NgoMIV GCCGGC 1 cut(s) 1652
NmeAIII GCCGAG 2 cut(s) 450, 542
NmuCI GTSAC 7 cut(s) 59, 1215, 2120, 2594, 2680, 3058, 3260
NspI RCATGY 3 cut(s) 214, 907, 2270
PaeR7I CTCGAG 1 cut(s) 2733
PceI AGGCCT 1 cut(s) 2669
PciI ACATGT 1 cut(s) 903
PciSI GCTCTTC 1 cut(s) 1720
PctI GAATGC 2 cut(s) 2475, 2639
PdiI GCCGGC 1 cut(s) 1654
PdmI GAANNNNTTC 2 cut(s) 1091, 3096
PfeI GAWTC 8 cut(s) 394, 581, 716, 736, 1117, 2543, 2583, 2824
PflFI GACNNNGTC 1 cut(s) 170
PflMI CCANNNNNTGG 2 cut(s) 1029, 1483
PfoI TCCNGGA 1 cut(s) 132
PinAI ACCGGT 1 cut(s) 1700
PleI GAGTC 7 cut(s) 85, 131, 454, 622, 1733, 1993, 2752
PpsI GAGTC 7 cut(s) 85, 131, 454, 622, 1733, 1993, 2752
Ppu21I YACGTR 3 cut(s) 1220, 1445, 2502
PpuMI RGGWCCY 1 cut(s) 16
PscI ACATGT 1 cut(s) 903
PsiI TTATAA 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 16
Psp6I CCWGG 4 cut(s) 1615, 1751, 1946, 2868
PspEI GGTNACC 1 cut(s) 44
PspFI CCCAGC 1 cut(s) 1756
PspGI CCWGG 4 cut(s) 1615, 1751, 1946, 2868
PspPI GGNCC 7 cut(s) 16, 441, 776, 945, 1486, 1619, 2966
PspPPI RGGWCCY 1 cut(s) 16
PspXI VCTCGAGB 1 cut(s) 2733
PstI CTGCAG 5 cut(s) 1552, 1843, 2026, 2245, 2290
PstNI CAGNNNCTG 1 cut(s) 1247
PsuI RGATCY 3 cut(s) 378, 811, 926
PsyI GACNNNGTC 1 cut(s) 170
PvuII CAGCTG 3 cut(s) 1154, 2225, 3026
RseI CAYNNNNRTG 6 cut(s) 687, 1541, 1973, 2678, 3158, 3204
SapI GCTCTTC 1 cut(s) 1720
Sau96I GGNCC 7 cut(s) 16, 441, 776, 945, 1486, 1619, 2966
SchI GAGTC 7 cut(s) 85, 131, 454, 623, 1733, 1993, 2753
ScrFI CCNGG 6 cut(s) 134, 1617, 1753, 1948, 2099, 2870
SduI GDGCHC 4 cut(s) 497, 1643, 1763, 3081
SfaNI GCATC 7 cut(s) 1054, 1234, 1739, 2008, 2286, 2527, 2905
SfcI CTRYAG 7 cut(s) 1548, 1839, 1854, 2022, 2241, 2286, 2640
Sfr274I CTCGAG 1 cut(s) 2733
SinI GGWCC 5 cut(s) 16, 945, 1486, 1619, 2966
SlaI CTCGAG 1 cut(s) 2733
SmiMI CAYNNNNRTG 6 cut(s) 687, 1541, 1973, 2678, 3158, 3204
SmlI CTYRAG 5 cut(s) 547, 1490, 1862, 2733, 3008
SmoI CTYRAG 5 cut(s) 547, 1490, 1862, 2733, 3008
SnaBI TACGTA 1 cut(s) 1445
SseBI AGGCCT 1 cut(s) 2669
SspI AATATT 1 cut(s) 1695
SspMI CTAG 3 cut(s) 2229, 2379, 3089
StuI AGGCCT 1 cut(s) 2669
StyD4I CCNGG 6 cut(s) 132, 1615, 1751, 1946, 2097, 2868
StyI CCWWGG 3 cut(s) 941, 1572, 2464
TaiI ACGT 5 cut(s) 174, 1222, 1447, 1613, 2504
TatI WGTACW 6 cut(s) 558, 1097, 1343, 1360, 1430, 2843
TauI GCSGC 5 cut(s) 404, 424, 569, 2157, 2604
TfiI GAWTC 8 cut(s) 394, 581, 716, 736, 1117, 2543, 2583, 2824
TscAI CASTG 6 cut(s) 367, 1269, 1311, 1889, 2695, 3262
TseFI GTSAC 7 cut(s) 59, 1215, 2120, 2594, 2680, 3058, 3260
Tsp45I GTSAC 7 cut(s) 59, 1215, 2120, 2594, 2680, 3058, 3260
TspDTI ATGAA 7 cut(s) 21, 1122, 1932, 2226, 2388, 3056, 3264
TspGWI ACGGA 4 cut(s) 229, 536, 729, 2696
TspRI CASTG 6 cut(s) 367, 1269, 1311, 1889, 2695, 3262
Tth111I GACNNNGTC 1 cut(s) 170
Van91I CCANNNNNTGG 2 cut(s) 1029, 1483
VpaK11BI GGWCC 5 cut(s) 16, 945, 1486, 1619, 2966
XapI RAATTY 3 cut(s) 71, 1661, 1684
XceI RCATGY 3 cut(s) 214, 907, 2270
XcmI CCANNNNNNNNNTGG 2 cut(s) 512, 3120
XhoI CTCGAG 1 cut(s) 2733
XmiI GTMKAC 1 cut(s) 1387
XmnI GAANNNNTTC 2 cut(s) 1091, 3096
XspI CTAG 3 cut(s) 2229, 2379, 3089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.