Rh3BG000300

Type II inositol 1,4,5-trisphosphate 5-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0010730
Physical Location & Seq
Reverse (-)
61860 .. 71958
10099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG000300.1

Sequence Viewer

Length: 3345 bp
ATGGAAGAAAACGACGGTTTCTTATCGTCTCTGAGCCTCCGTAACTCCTCCGCGCCAATACCAAGACCAAACCAAAACGATGGTCTCGACGGCGGCAAACTCTTCGTGGACTCTTCACCATCTTCATCGGAAGATGAAAATGACGCCGCCTCCCTCCACTCCACTTCAAAACGCCTCGACTATATGCTCCAATTCCTCGACCGCAAGCTCTCCGTTGCCAACGTCAACGTCGTCGACCACATCAACAATATCAATATGAGCGACGCCGCCGACACTGCTCACGGCTCCTCCTCGCTCCCTGAGTTCGAGGCCAAGGGCGGAGGTACCGGAATGTTCAAAGTCCCGGTTCGGGGGGCGGTCCACCCGAACCGGCCGCCAAGGCTGGAACTGAGGCCCCAACCGCTGAGGGAGACTCAGATAGGATGCTTCTTGCGGACTATGGTCAGCACCGAGTCCCAATTGTGGGCTGGGACGGAGTGCGCCGTGAGGGTTTGGAATTTTAAAGATTTGTACTCGCCGGCGGCGGCTGAGGCTGGACGAGGGGAAGGCGATTCGGAAACGGCACCGTTTAGGGAGTCCGTGTGCACATCCGCGGTGATGTGTTTAGTTGGGGATGATGGGAATAGGGTGGTGTGGAGTGGACATAGGGATGGTAAAATTAGGTGTTGGAGGATGGACTCCTCCTCCTCAGCACTAGTAACTGCTCCTTTTAAGGAGGGCTTGTCTTGGCAGGCTCATAGAGGCCCCGTTCTTTCACTTGTTATTTCTTGTTACGGGGATCTCTGGTCAGGTTCAGAGGGTGGTGTCATCAAGATATGGCCATGGGAAGCTATTGAAAAAGCTCTGTCTCTTACCATTGAAGAAAGGCACATGTCTTCTTTGTTGGTGGAGAGGTCTTACATTGATCCCTGGACCCAAGTTGCCGTCAATGGTTTCACTAATGTATTAACTTCAGATGTTAGGTACTTATTATCTGATAGCTCCAGTGCAAAGGTGTGGAGTGCTGGTTATCTCTCATTCGCACTGTGGGATGCGCGCACAAGAGAATTACTGAAAGTGTTCAATACCGATGGTCTGAATGAGAATCGAGTGGACATTTCATCAGCACAGGACTTGTCAGTGGAATTTATTTCTGGAGCAAAAAAAGACAAAACACAAAGTTCTTTTGGTTTCTTTCAGCGATCACGTAATGCTTTAATGGGAGCAGCAGATGCTGTTCGTCGAGTTGCAGTGAAGGGTGCCTTTGGAGATGATAATCGAAGAACTGAAGCATTGGTAATTGCAGTAGATACAATGATTTGGACGGGGTGTGCAAATGGCCTACTTGTGCAGTGGGATAGAAATGGCAACCGTATGCAAGAATTCCATTACCATTCTTCTGCTGTCCAATGCTTTTGTACTTTTGGGCTACGTATATGGGTGGGTTATGCAAGTGGCACTGTCCAGGTATTGGACCTCAACGGTAATCTGCTTGGAGGATGGGTAGCTGACAACAGTCCTATTATTAAAATAGCTACAGGAGCTGGTTATGTATTTACCTTGGCCAATCATGGTGGTATATGTGGATGGAATATTACATCACCGGGACCTCTTGACAGCATAGTGCGTTCAGAATTAGCTGGCAAAGAATTTCTATATACAAGGATAGAAAACTTGAAAATATTGACTGGTACATGGAACGTTGGACAAGGACGAGCGTCTCAGGACTCACTTATATCCTGGCTGGGCTCTGTGGCTTCTGATGTTGGCATTGTTGTTGTTGGTTTGCAAGAAGTTGAAATGGGTGCTGGTTTTCTTGCTATGTCTGCAGCCAAAGAAACTGTAGGTCTTGAGGGGAGCTCTGTTGGGCAGTGGTGGCTGGATATGATTGGAAAAGCATTGGATGAAGGGTCAACCTTTGAACGTGTTGGATCCAGACAGTTGGCAGGATTGCTCATTGCTATGTGGGTGAGACATAATCTCAAAGCTCATGTTGGAGATGTCGATGCTGCAGCAGTTCCGTGTGGTTTTGGGCGCGCTATTGGTAACAAGGGAGCTGTTGGTTTGAGGATTAGATTGTATGACAGGATAATGTGCTTTGTTAATTGTCACTTTGCTGCGCATTTGGAAGCTGTTAATCGCCGCAATGCAGATTTCGATCATGTATATCGAACCATGACCTTCTCCCGACCTAATTTTCTCAATTGTGCAGCTGCTAGTGCTTCATCTGCTGTTCAAATGCTTCGCGGTACAAATGCTATTGGTAACAATTCTGTAGAAGGGATGCCGGAGTTATCTGAAGCAGACATGGTCATATTTCTTGGTGATTTTAACTATCGGCTTGATGGTATATCTTATGATGAAGCTAGAGATTTTGTTTCTCAAAGATGCTTTGATTGGCTTAGAGAACGGGATCAGCTCCGAGTGGAAATGGCAGCTGGAAATGTCTTTCAAGGAATGCGGGAGGCAGATATTACATTTCCTCCCACATACAAGTTTGAAAGGCATCAAGCTGGTTTAGCAGGGTATGATTCCGGTGAAAAGAAGCGCATTCCAGCTTGGTGTGATAGAATCCTGCATCGTGATAGCCGCTCAGCGTTGGTATCAGAATGCTGCTTAGAATGTCCTGTAGTCTCTTCAATATCACAGTACGAGGCTTCCATGGATGTAACCGACAGCGATCACAAGCCTGTCCGCTGCATTTTTACTCTTGATATTGCTCGAGTTGATGAGTCAATAAGGAGACAAGAGCTTGGAGATATCCTTGAATCAAATGGGAAACTCAAATGTATGCTTGAAGAACTATGTAGGATTCCTGAAACAATTGTCAGTACAAACAAAATAATCCTTCAAATCCAGGACACATCCATTTTGCGTATTACAAATAAATCTGGCCAAAAAGATGCTTTATTTGAAATAATTTGTGAAGGACAATCTATCATTAAGGAGGATGGACATGCATCAGATCATTGTCCCAGAGGTTCCTTTGGCTTTCCGCGATGGCTTCAGGTCACTCCTGCAGCTGGCATAATCAGACCAGATCATATAGCAGAGGTGTCTGTTCATCATGAGGAACACCAAACCCTTGAAGAGTTTGTTGATGGTGTCCCGCAGAACTGTTGGTGTGAAAACACCAGAGACAAGGAAGTTATACTGGTGGTTAAGGTACACGGGAGGTACAGTAATAACACCAAAAGCCATCGCGTCTGTGTGCGTCACTGCTGTTCAGCCAAGACAAAGCAAAGTGACCCCCCAGAGCACAACACCAGACAAACCCAAGGAACCGCTCTTCACCGTTCCAACTTCCAACACCTTAGCAGTTCCTATGACGTGGTTGACCACCTGTGGAGTATGAATAGTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

1114

Amino Acids

122.93

Weight (kDa)

5.82

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_IP5PC_F PF23754 135 - 523 2.2e-187 IP5P C-F beta-propeller
Exo_endo_phos2 PF22669 557 - 892 1.3e-65 Endonuclease/Exonuclease/phosphatase family 2
Exo_endo_phos PF03372 557 - 888 6.5e-07 Endonuclease/Exonuclease/phosphatase family
Ig-like_IP5PC_F PF23755 931 - 1067 3e-70 IP5P C-F Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010730)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65580 AT1G65580
fragaria_vesca FvH4_6g00030 FvH4_6g00030
malus_domestica MD12G1260800.v1.1
prunus_persica Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1
pyrus_communis pycom12g24280
rosa_chinensis RchiOBHm_Chr3g0447351
rosa_laevigata RLG00000026007
rosa_multiflora Rmu_ssc0000473.1_g000002
rosa_roxburghii Rroxscaffold_6G00425530
rosa_rugosa Rorug02G0600200 Rorug02G0600300
rosa_samantha Rh3AG000500 Rh3BG000300 Rh3CG000300 Rh3DG000500
rosa_wichuraiana Rw3G000030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2100
Acc65I GGTACC 1 cut(s) 323
AccB1I GGYRCC 3 cut(s) 323, 562, 1238
AccB7I CCANNNNNTGG 1 cut(s) 1450
AccBSI CCGCTC 2 cut(s) 2571, 3266
AccI GTMKAC 1 cut(s) 234
AccII CGCG 7 cut(s) 53, 593, 1036, 2016, 2226, 2977, 3183
AclI AACGTT 1 cut(s) 1680
AclWI GGATC 5 cut(s) 786, 899, 1905, 1918, 2400
AcoI YGGCCR 4 cut(s) 371, 818, 1542, 2872
AcsI RAATTY 4 cut(s) 496, 1124, 1361, 1628
AcuI CTGAAG 4 cut(s) 936, 1287, 2298, 2969
AcyI GRCGYC 2 cut(s) 144, 264
AfiI CCNNNNNNNGG 6 cut(s) 349, 350, 462, 463, 1450, 3002
AflIII ACRYGT 2 cut(s) 870, 1903
AhlI ACTAGT 1 cut(s) 694
AjiI CACGTC 1 cut(s) 3310
AjnI CCWGG 4 cut(s) 908, 1443, 1718, 2835
Alw21I GWGCWC 3 cut(s) 587, 1841, 3240
Alw26I GTCTC 9 cut(s) 33, 89, 404, 852, 1704, 1945, 2617, 2716, 3111
Alw44I GTGCAC 1 cut(s) 583
AlwI GGATC 5 cut(s) 786, 899, 1905, 1918, 2400
AlwNI CAGNNNCTG 2 cut(s) 1214, 1523
Ama87I CYCGRG 1 cut(s) 2700
AoxI GGCC 8 cut(s) 309, 371, 392, 742, 818, 1318, 1542, 2872
ApaLI GTGCAC 1 cut(s) 583
ApoI RAATTY 4 cut(s) 496, 1124, 1361, 1628
ArsI GACNNNNNNTTYG 2 cut(s) 67, 99
Asp700I GAANNNNTTC 1 cut(s) 1058
Asp718I GGTACC 1 cut(s) 323
AspLEI GCGC 8 cut(s) 55, 482, 1036, 1038, 2016, 2018, 2101, 2529
AspS9I GGNCC 6 cut(s) 358, 393, 743, 912, 1453, 1586
AsuC2I CCSGG 2 cut(s) 344, 1584
AsuHPI GGTGA 7 cut(s) 108, 607, 1572, 1960, 2315, 2528, 3263
AvaI CYCGRG 1 cut(s) 2700
AvaII GGWCC 4 cut(s) 358, 912, 1453, 1586
BaeGI GKGCMC 1 cut(s) 587
BalI TGGCCA 3 cut(s) 820, 1544, 2874
BamHI GGATCC 1 cut(s) 1910
BanI GGYRCC 3 cut(s) 323, 562, 1238
BanII GRGCYC 2 cut(s) 1730, 1841
BbsI GAAGAC 1 cut(s) 867
Bbv12I GWGCWC 3 cut(s) 587, 1841, 3240
BbvCI CCTCAGC 3 cut(s) 404, 528, 688
BceAI ACGGC 5 cut(s) 106, 298, 467, 576, 908
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 4 cut(s) 910, 1445, 1720, 2837
BcnI CCSGG 2 cut(s) 344, 1584
BcoDI GTCTC 9 cut(s) 33, 89, 404, 852, 1704, 1945, 2617, 2716, 3111
BcuI ACTAGT 1 cut(s) 694
BfaI CTAG 3 cut(s) 695, 2196, 2346
BfmI CTRYAG 7 cut(s) 1515, 1806, 1821, 1989, 2253, 2607, 2997
BglI GCCNNNNNGGC 1 cut(s) 379
BlpI GCTNAGC 1 cut(s) 2572
Bme1390I CCNGG 6 cut(s) 344, 910, 1445, 1584, 1720, 2837
Bme18I GGWCC 4 cut(s) 358, 912, 1453, 1586
BmeT110I CYCGRG 1 cut(s) 2700
BmgBI CACGTC 1 cut(s) 3310
BmgT120I GGNCC 6 cut(s) 358, 393, 743, 912, 1453, 1586
BmrFI CCNGG 6 cut(s) 344, 910, 1445, 1584, 1720, 2837
BoxI GACNNNNGTC 3 cut(s) 440, 1494, 1696
BpiI GAAGAC 1 cut(s) 867
BplI GAGNNNNNCTC 4 cut(s) 397, 429, 1823, 1855
BpmI CTGGAG 2 cut(s) 967, 1155
Bpu10I CCTNAGC 4 cut(s) 404, 528, 688, 3293
Bpu1102I GCTNAGC 1 cut(s) 2572
BpuEI CTTGAG 1 cut(s) 1850
BpuMI CCSGG 2 cut(s) 344, 1584
BsaAI YACGTR 2 cut(s) 1187, 1412
BsaBI GATNNNNATC 2 cut(s) 1254, 2136
BsaHI GRCGYC 2 cut(s) 144, 264
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 8 cut(s) 312, 377, 591, 821, 908, 1539, 2640, 3256
BsaWI WCCGGW 2 cut(s) 326, 2513
Bsc4I CCNNNNNNNGG 6 cut(s) 349, 350, 462, 463, 1450, 3002
Bse118I RCCGGY 2 cut(s) 369, 517
Bse1I ACTGG 3 cut(s) 984, 1672, 3138
Bse3DI GCAATG 2 cut(s) 1935, 2131
Bse8I GATNNNNATC 2 cut(s) 1254, 2136
BseBI CCWGG 4 cut(s) 910, 1445, 1720, 2837
BseDI CCNNGG 8 cut(s) 312, 377, 591, 821, 908, 1539, 2640, 3256
BseJI GATNNNNATC 2 cut(s) 1254, 2136
BseLI CCNNNNNNNGG 6 cut(s) 349, 350, 462, 463, 1450, 3002
BseMI GCAATG 2 cut(s) 1935, 2131
BseMII CTCAG 9 cut(s) 23, 291, 380, 395, 428, 519, 702, 1715, 2586
BseNI ACTGG 3 cut(s) 984, 1672, 3138
BsePI GCGCGC 2 cut(s) 1034, 2014
BseRI GAGGAG 6 cut(s) 37, 277, 280, 670, 673, 676
BseSI GKGCMC 1 cut(s) 587
BseX3I CGGCCG 1 cut(s) 371
BseYI CCCAGC 2 cut(s) 467, 1723
BsgI GTGCAG 2 cut(s) 1349, 2208
Bsh1236I CGCG 7 cut(s) 53, 593, 1036, 2016, 2226, 2977, 3183
Bsh1285I CGRYCG 2 cut(s) 202, 374
BshFI GGCC 8 cut(s) 311, 373, 394, 744, 820, 1320, 1544, 2874
BshNI GGYRCC 3 cut(s) 323, 562, 1238
BsiEI CGRYCG 2 cut(s) 202, 374
BsiHKAI GWGCWC 3 cut(s) 587, 1841, 3240
BsiHKCI CYCGRG 1 cut(s) 2700
BsiSI CCGG 7 cut(s) 327, 344, 370, 518, 1583, 2267, 2514
BslFI GGGAC 6 cut(s) 326, 439, 484, 1599, 2937, 3071
BslI CCNNNNNNNGG 6 cut(s) 349, 350, 462, 463, 1450, 3002
BsmAI GTCTC 9 cut(s) 33, 89, 404, 852, 1704, 1945, 2617, 2716, 3111
BsmBI CGTCTC 2 cut(s) 33, 1704
BsmFI GGGAC 6 cut(s) 326, 439, 484, 1599, 2937, 3071
BsmI GAATGC 3 cut(s) 2442, 2529, 2594
BsnI GGCC 8 cut(s) 311, 373, 394, 744, 820, 1320, 1544, 2874
Bso31I GGTCTC 1 cut(s) 89
BsoBI CYCGRG 1 cut(s) 2700
Bsp1286I GDGCHC 4 cut(s) 587, 1730, 1841, 3240
Bsp143I GATC 9 cut(s) 778, 904, 1181, 1910, 2137, 2392, 2659, 2944, 3019
Bsp1720I GCTNAGC 1 cut(s) 2572
Bsp19I CCATGG 2 cut(s) 821, 2640
BspANI GGCC 8 cut(s) 311, 373, 394, 744, 820, 1320, 1544, 2874
BspCNI CTCAG 9 cut(s) 24, 292, 381, 396, 427, 520, 701, 1714, 2585
BspFNI CGCG 7 cut(s) 53, 593, 1036, 2016, 2226, 2977, 3183
BspHI TCATGA 1 cut(s) 3046
BspMAI CTGCAG 3 cut(s) 1810, 1993, 3001
BspPI GGATC 5 cut(s) 786, 899, 1905, 1918, 2400
BspQI GCTCTTC 1 cut(s) 3273
BspT107I GGYRCC 3 cut(s) 323, 562, 1238
BspTNI GGTCTC 1 cut(s) 89
BsrBI CCGCTC 2 cut(s) 2571, 3266
BsrDI GCAATG 2 cut(s) 1935, 2131
BsrFI RCCGGY 2 cut(s) 369, 517
BsrI ACTGG 3 cut(s) 984, 1672, 3138
BssAI RCCGGY 2 cut(s) 369, 517
BssECI CCNNGG 8 cut(s) 312, 377, 591, 821, 908, 1539, 2640, 3256
BssHII GCGCGC 2 cut(s) 1034, 2014
BssMI GATC 9 cut(s) 778, 904, 1181, 1910, 2137, 2392, 2659, 2944, 3019
BssNI GRCGYC 2 cut(s) 144, 264
BssT1I CCWWGG 6 cut(s) 312, 377, 821, 1539, 2640, 3256
Bst2UI CCWGG 4 cut(s) 910, 1445, 1720, 2837
Bst6I CTCTTC 5 cut(s) 107, 118, 2620, 3063, 3273
BstACI GRCGYC 2 cut(s) 144, 264
BstAPI GCANNNNNTGC 1 cut(s) 1211
BstBAI YACGTR 2 cut(s) 1187, 1412
BstC8I GCNNGC 7 cut(s) 206, 519, 732, 1036, 1621, 2016, 3004
BstDSI CCRYGG 3 cut(s) 591, 821, 2640
BstFNI CGCG 7 cut(s) 53, 593, 1036, 2016, 2226, 2977, 3183
BstHHI GCGC 8 cut(s) 55, 482, 1036, 1038, 2016, 2018, 2101, 2529
BstKTI GATC 9 cut(s) 781, 907, 1184, 1913, 2140, 2395, 2662, 2947, 3022
BstMAI GTCTC 9 cut(s) 33, 89, 404, 852, 1704, 1945, 2617, 2716, 3111
BstMBI GATC 9 cut(s) 778, 904, 1181, 1910, 2137, 2392, 2659, 2944, 3019
BstMCI CGRYCG 2 cut(s) 202, 374
BstNI CCWGG 4 cut(s) 910, 1445, 1720, 2837
BstNSI RCATGY 2 cut(s) 874, 2939
BstPAI GACNNNNGTC 3 cut(s) 440, 1494, 1696
BstSCI CCNGG 6 cut(s) 342, 908, 1443, 1582, 1718, 2835
BstSFI CTRYAG 7 cut(s) 1515, 1806, 1821, 1989, 2253, 2607, 2997
BstSLI GKGCMC 1 cut(s) 587
BstSNI TACGTA 1 cut(s) 1412
BstUI CGCG 7 cut(s) 53, 593, 1036, 2016, 2226, 2977, 3183
BstV2I GAAGAC 1 cut(s) 867
BstX2I RGATCY 2 cut(s) 778, 1910
BstXI CCANNNNNNTGG 2 cut(s) 80, 1921
BstYI RGATCY 2 cut(s) 778, 1910
BstZI CGGCCG 1 cut(s) 371
BsuRI GGCC 8 cut(s) 311, 373, 394, 744, 820, 1320, 1544, 2874
BtgI CCRYGG 3 cut(s) 591, 821, 2640
BtgZI GCGATG 2 cut(s) 2992, 3164
BtrI CACGTC 1 cut(s) 3310
BtsI GCAGTG 5 cut(s) 273, 1236, 1337, 1856, 3196
BtsIMutI CAGTG 9 cut(s) 273, 991, 1022, 1125, 1236, 1337, 1437, 1856, 3196
Cac8I GCNNGC 7 cut(s) 206, 519, 732, 1036, 1621, 2016, 3004
CaiI CAGNNNCTG 2 cut(s) 1214, 1523
CciI TCATGA 1 cut(s) 3046
CfoI GCGC 8 cut(s) 55, 482, 1036, 1038, 2016, 2018, 2101, 2529
Cfr10I RCCGGY 2 cut(s) 369, 517
Cfr13I GGNCC 6 cut(s) 358, 393, 743, 912, 1453, 1586
Cfr42I CCGCGG 1 cut(s) 594
CseI GACGC 5 cut(s) 152, 272, 1686, 3172, 3182
CspCI CAANNNNNGTGG 2 cut(s) 1534, 1569
DpnI GATC 9 cut(s) 780, 906, 1183, 1912, 2139, 2394, 2661, 2946, 3021
DpnII GATC 9 cut(s) 778, 904, 1181, 1910, 2137, 2392, 2659, 2944, 3019
DraI TTTAAA 1 cut(s) 502
EaeI YGGCCR 4 cut(s) 371, 818, 1542, 2872
EagI CGGCCG 1 cut(s) 371
Eam1104I CTCTTC 5 cut(s) 107, 118, 2620, 3063, 3273
EarI CTCTTC 5 cut(s) 107, 118, 2620, 3063, 3273
EciI GGCGGA 1 cut(s) 333
Ecl136II GAGCTC 1 cut(s) 1839
EclXI CGGCCG 1 cut(s) 371
Eco105I TACGTA 1 cut(s) 1412
Eco130I CCWWGG 6 cut(s) 312, 377, 821, 1539, 2640, 3256
Eco24I GRGCYC 2 cut(s) 1730, 1841
Eco31I GGTCTC 1 cut(s) 89
Eco32I GATATC 1 cut(s) 2740
Eco47I GGWCC 4 cut(s) 358, 912, 1453, 1586
Eco52I CGGCCG 1 cut(s) 371
Eco53kI GAGCTC 1 cut(s) 1839
Eco57I CTGAAG 4 cut(s) 936, 1287, 2298, 2969
Eco88I CYCGRG 1 cut(s) 2700
EcoICRI GAGCTC 1 cut(s) 1839
EcoO109I RGGNCCY 3 cut(s) 393, 743, 1586
EcoRI GAATTC 1 cut(s) 1361
EcoRII CCWGG 4 cut(s) 908, 1443, 1718, 2835
EcoRV GATATC 1 cut(s) 2740
EcoT14I CCWWGG 6 cut(s) 312, 377, 821, 1539, 2640, 3256
EcoT22I ATGCAT 1 cut(s) 2941
EcoT38I GRGCYC 2 cut(s) 1730, 1841
ErhI CCWWGG 6 cut(s) 312, 377, 821, 1539, 2640, 3256
Esp3I CGTCTC 2 cut(s) 33, 1704
FalI AAGNNNNNCTT 4 cut(s) 704, 736, 1226, 1258
FaqI GGGAC 6 cut(s) 326, 439, 484, 1599, 2937, 3071
FauI CCCGC 2 cut(s) 2433, 3096
FblI GTMKAC 1 cut(s) 234
FriOI GRGCYC 2 cut(s) 1730, 1841
FspBI CTAG 3 cut(s) 695, 2196, 2346
FspI TGCGCA 1 cut(s) 2100
GlaI GCGC 8 cut(s) 54, 481, 1035, 1037, 2015, 2017, 2100, 2528
GsaI CCCAGC 2 cut(s) 471, 1727
GsuI CTGGAG 2 cut(s) 967, 1155
HaeIII GGCC 8 cut(s) 311, 373, 394, 744, 820, 1320, 1544, 2874
HapII CCGG 7 cut(s) 327, 344, 370, 518, 1583, 2267, 2514
HgaI GACGC 5 cut(s) 152, 272, 1686, 3172, 3182
HhaI GCGC 8 cut(s) 55, 482, 1036, 1038, 2016, 2018, 2101, 2529
Hin1I GRCGYC 2 cut(s) 144, 264
Hin6I GCGC 8 cut(s) 53, 480, 1034, 1036, 2014, 2016, 2099, 2527
HinP1I GCGC 8 cut(s) 53, 480, 1034, 1036, 2014, 2016, 2099, 2527
HincII GTYRAC 4 cut(s) 226, 235, 1893, 3316
HindII GTYRAC 4 cut(s) 226, 235, 1893, 3316
HpaII CCGG 7 cut(s) 327, 344, 370, 518, 1583, 2267, 2514
HphI GGTGA 7 cut(s) 108, 607, 1572, 1960, 2315, 2528, 3263
Hpy99I CGWCG 6 cut(s) 17, 92, 233, 236, 266, 1224
HpyAV CCTTC 7 cut(s) 539, 1228, 1880, 2170, 2252, 2837, 2900
HpyCH4IV ACGT 7 cut(s) 222, 228, 1186, 1411, 1680, 1903, 3309
HpySE526I ACGT 7 cut(s) 222, 228, 1186, 1411, 1680, 1903, 3309
Hsp92I GRCGYC 2 cut(s) 144, 264
HspAI GCGC 8 cut(s) 53, 480, 1034, 1036, 2014, 2016, 2099, 2527
KpnI GGTACC 1 cut(s) 327
KroI GCCGGC 1 cut(s) 517
KroNI GCCGGC 1 cut(s) 519
KspI CCGCGG 1 cut(s) 594
Kzo9I GATC 9 cut(s) 778, 904, 1181, 1910, 2137, 2392, 2659, 2944, 3019
LguI GCTCTTC 1 cut(s) 3273
MaeI CTAG 3 cut(s) 695, 2196, 2346
MaeII ACGT 7 cut(s) 222, 228, 1186, 1411, 1680, 1903, 3309
MalI GATC 9 cut(s) 780, 906, 1183, 1912, 2139, 2394, 2661, 2946, 3021
MbiI CCGCTC 2 cut(s) 2571, 3266
MboI GATC 9 cut(s) 778, 904, 1181, 1910, 2137, 2392, 2659, 2944, 3019
MfeI CAATTG 3 cut(s) 458, 2182, 2802
MflI RGATCY 2 cut(s) 778, 1910
MhlI GDGCHC 4 cut(s) 587, 1730, 1841, 3240
MlsI TGGCCA 3 cut(s) 820, 1544, 2874
MluNI TGGCCA 3 cut(s) 820, 1544, 2874
MlyI GAGTC 7 cut(s) 104, 406, 461, 584, 671, 1700, 2720
MmeI TCCRAC 6 cut(s) 647, 1663, 1888, 1954, 3303, 3310
Mox20I TGGCCA 3 cut(s) 820, 1544, 2874
Mph1103I ATGCAT 1 cut(s) 2941
MreI CGCCGGCG 1 cut(s) 517
MroNI GCCGGC 1 cut(s) 517
MroXI GAANNNNTTC 1 cut(s) 1058
MscI TGGCCA 3 cut(s) 820, 1544, 2874
MslI CAYNNNNRTG 2 cut(s) 648, 1940
Msp20I TGGCCA 3 cut(s) 820, 1544, 2874
MspA1I CMGCKG 6 cut(s) 403, 593, 2192, 2417, 2676, 3002
MspI CCGG 7 cut(s) 327, 344, 370, 518, 1583, 2267, 2514
MspR9I CCNGG 6 cut(s) 344, 910, 1445, 1584, 1720, 2837
MunI CAATTG 3 cut(s) 458, 2182, 2802
Mva1269I GAATGC 3 cut(s) 2442, 2529, 2594
MvaI CCWGG 4 cut(s) 910, 1445, 1720, 2837
MvnI CGCG 7 cut(s) 53, 593, 1036, 2016, 2226, 2977, 3183
NaeI GCCGGC 1 cut(s) 519
NciI CCSGG 2 cut(s) 344, 1584
NcoI CCATGG 2 cut(s) 821, 2640
NdeII GATC 9 cut(s) 778, 904, 1181, 1910, 2137, 2392, 2659, 2944, 3019
NgoMIV GCCGGC 1 cut(s) 517
NmuCI GTSAC 4 cut(s) 2087, 2989, 3194, 3224
NsbI TGCGCA 1 cut(s) 2100
NsiI ATGCAT 1 cut(s) 2941
NspI RCATGY 2 cut(s) 874, 2939
PaeR7I CTCGAG 1 cut(s) 2700
PagI TCATGA 1 cut(s) 3046
PauI GCGCGC 2 cut(s) 1034, 2014
PciI ACATGT 1 cut(s) 870
PciSI GCTCTTC 1 cut(s) 3273
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 228
PctI GAATGC 3 cut(s) 2442, 2529, 2594
PdiI GCCGGC 1 cut(s) 519
PdmI GAANNNNTTC 1 cut(s) 1058
PfeI GAWTC 6 cut(s) 551, 1084, 2510, 2550, 2747, 2791
PflFI GACNNNGTC 1 cut(s) 2288
PflMI CCANNNNNTGG 1 cut(s) 1450
PfoI TCCNGGA 1 cut(s) 2835
PleI GAGTC 7 cut(s) 104, 406, 460, 583, 671, 1700, 2719
PpsI GAGTC 7 cut(s) 104, 406, 460, 583, 671, 1700, 2719
Ppu21I YACGTR 2 cut(s) 1187, 1412
PpuMI RGGWCCY 1 cut(s) 1586
PscI ACATGT 1 cut(s) 870
PshAI GACNNNNGTC 3 cut(s) 440, 1494, 1696
Psp124BI GAGCTC 1 cut(s) 1841
Psp1406I AACGTT 1 cut(s) 1680
Psp5II RGGWCCY 1 cut(s) 1586
Psp6I CCWGG 4 cut(s) 908, 1443, 1718, 2835
PspFI CCCAGC 2 cut(s) 467, 1723
PspGI CCWGG 4 cut(s) 908, 1443, 1718, 2835
PspPI GGNCC 6 cut(s) 358, 393, 743, 912, 1453, 1586
PspPPI RGGWCCY 1 cut(s) 1586
PspXI VCTCGAGB 1 cut(s) 2700
PstI CTGCAG 3 cut(s) 1810, 1993, 3001
PstNI CAGNNNCTG 2 cut(s) 1214, 1523
PsuI RGATCY 2 cut(s) 778, 1910
PsyI GACNNNGTC 1 cut(s) 2288
PteI GCGCGC 2 cut(s) 1034, 2014
PvuII CAGCTG 3 cut(s) 2192, 2417, 3002
RseI CAYNNNNRTG 2 cut(s) 648, 1940
SacI GAGCTC 1 cut(s) 1841
SacII CCGCGG 1 cut(s) 594
SalI GTCGAC 1 cut(s) 233
SapI GCTCTTC 1 cut(s) 3273
Sau3AI GATC 9 cut(s) 778, 904, 1181, 1910, 2137, 2392, 2659, 2944, 3019
Sau96I GGNCC 6 cut(s) 358, 393, 743, 912, 1453, 1586
SchI GAGTC 7 cut(s) 104, 406, 461, 584, 671, 1700, 2720
ScrFI CCNGG 6 cut(s) 344, 910, 1445, 1584, 1720, 2837
SduI GDGCHC 4 cut(s) 587, 1730, 1841, 3240
SfcI CTRYAG 7 cut(s) 1515, 1806, 1821, 1989, 2253, 2607, 2997
Sfr274I CTCGAG 1 cut(s) 2700
Sfr303I CCGCGG 1 cut(s) 594
SgrAI CRCCGGYG 1 cut(s) 517
SgrBI CCGCGG 1 cut(s) 594
SinI GGWCC 4 cut(s) 358, 912, 1453, 1586
SlaI CTCGAG 1 cut(s) 2700
SmiMI CAYNNNNRTG 2 cut(s) 648, 1940
SmlI CTYRAG 2 cut(s) 1829, 2700
SmoI CTYRAG 2 cut(s) 1829, 2700
SnaBI TACGTA 1 cut(s) 1412
SpeI ACTAGT 1 cut(s) 694
SspI AATATT 2 cut(s) 1573, 1662
SspMI CTAG 3 cut(s) 695, 2196, 2346
SstI GAGCTC 1 cut(s) 1841
StyD4I CCNGG 6 cut(s) 342, 908, 1443, 1582, 1718, 2835
StyI CCWWGG 6 cut(s) 312, 377, 821, 1539, 2640, 3256
TaiI ACGT 7 cut(s) 225, 231, 1189, 1414, 1683, 1906, 3312
TatI WGTACW 3 cut(s) 510, 1397, 2810
TauI GCSGC 8 cut(s) 96, 149, 269, 376, 524, 527, 2124, 2571
TfiI GAWTC 6 cut(s) 551, 1084, 2510, 2550, 2747, 2791
TscAI CASTG 9 cut(s) 280, 991, 1029, 1125, 1236, 1337, 1444, 1856, 3203
TseFI GTSAC 4 cut(s) 2087, 2989, 3194, 3224
Tsp45I GTSAC 4 cut(s) 2087, 2989, 3194, 3224
TspDTI ATGAA 7 cut(s) 114, 150, 1089, 1899, 2193, 2355, 3032
TspGWI ACGGA 5 cut(s) 29, 202, 488, 568, 1989
TspRI CASTG 9 cut(s) 280, 991, 1029, 1125, 1236, 1337, 1444, 1856, 3203
Tth111I GACNNNGTC 1 cut(s) 2288
Van91I CCANNNNNTGG 1 cut(s) 1450
VneI GTGCAC 1 cut(s) 583
VpaK11BI GGWCC 4 cut(s) 358, 912, 1453, 1586
XapI RAATTY 4 cut(s) 496, 1124, 1361, 1628
XceI RCATGY 2 cut(s) 874, 2939
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XhoI CTCGAG 1 cut(s) 2700
XmiI GTMKAC 1 cut(s) 234
XmnI GAANNNNTTC 1 cut(s) 1058
XspI CTAG 3 cut(s) 695, 2196, 2346
Zsp2I ATGCAT 1 cut(s) 2941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.