Rw3G000030

Type II inositol 1,4,5-trisphosphate 5-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0010730
Physical Location & Seq
Reverse (-)
66316 .. 76010
9695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G000030.1

Sequence Viewer

Length: 3351 bp
ATGGAAGAAAACGACGGTTTCTTATCGTCTCTGAGCCTCCGTAACTCCTCCGCGCCAATACCAAGACCAAACCAAAACGATGGTCTCGACGGCGGCAAACTCTTCGTGGACTCTTCACCATCTTCATCGGAAGATGAAAATGACGCCGTCTCCCTCCACTCCACTTCAAAACGCCTCGACTATATGCTCCAATTCCTCGACCGCAAGCTCTCCGTTGCCAACGTCAACGTCAACGTCGTCGACCACATCAACAATATCAATATGAGCGACGCCGCCGACACTGCTCAGGGCTCCTCCTCGCTCCCTGAGTTCGAGGCCAAGGGCGGAGGTACCGGAATGTTCAAAGTCCCGGTTCGGGGGGCGGTCCACCCGAACCGGCCGCCAAGGCTGGAACTGAGGCCCCAACCGCTGAGGGAGACTCAGATAGGATGCTTCTTGCGGACTATGGTCAGCACCGAGTCCCAATTGTGGGCTGGGACGGAGTGCGCCGTCAGGGTTTGGAATTTTAAAGATTTGTACTCGCCGGCGGCGGCTGAGGCTGGACGAGGGGAAGGCGATTCGGAAACGGCACCGTTTAGGGAGTCCGTGTGCACATCCGCGGTGATGTGTTTAGTTGGGGATGATGGGAATAGGGTGGTGTGGAGTGGACATAGGGATGGTAAAATTAGGTGTTGGAGGATGGACTCCTCCTCCTCAGCACTAGTAACTGCTCCTTTTAAGGAGGGCTTGTCTTGGCAGGCTCATAGAGGCCCCGTTCTTTCACTTGTTATTTCTTGTTACGGGGATCTCTGGTCAGGTTCAGAGGGTGGTGTCATCAAGATATGGCCATGGGAAGCTATTGAAAAAGCTCTGTCTCTTACCATTGAAGAAAGGCACATGTCTTCTTTGTTGGTGGAGAGGTCTTACATTGATCCCTGGACCCAAGTTGCTGTCAATGGTTTCACTAATGTATTAACTTCAGATGTTAGGTATTTATTATCTGATAGCTCCAGTGCAAAGGTGTGGAGTGCTGGTTATCTCTCATTCGCACTGTGGGATGCGCGCACAAGAGAATTACTAAAAGTGTTCAATACCGATGGTCTGAATGAGAATCGAGTGGACATTTCATCAGCACAGGACTTGTCAGTGGAATTTATTTCTGGAGCAAAAAAAGACAAAACACAAAGTTCTTTTGGTTTCTTTCAGCGATCACGTAATGCTTTAATGGGAGCAGCAGATGCTGTTCGTCGAGTTGCAGTGAAGGGTGCCTTTGGAGATGATAATCGAAGAACTGAAGCATTGGTAATTGCAGTAGATACAATGATTTGGACGGGGTGTGCAAATGGCCTACTTGTGCAGTGGGATAGAAATGGCAACCGTATGCAAGAATTCCATTACCATTCTTCTGCTGTCCAATGCTTTTGTACTTTTGGGCTACGTATATGGGTGGGTTATGCAAGTGGCACTGTCCAGGTATTGGACCTCGACGGTAATCTGCTTGGAGGATGGGTAGCTGACAACAGTCCTATTATTAAAATAGCTACAGGAGCTGGTTATGTATTTACCTTGGCCAATCATGGTGGTATATGTGGATGGAATATTACATCACCGGGACCTCTTGACAGCATAGTGCGTTCAGAATTAGCTGGCAAAGAATTTCTATATACAAGGATAGAAAACTTGAAAATATTGACTGGTACATGGAACGTTGGACAAGGACGAGCGTCACAGGACTCACTTATATCCTGGCTGGGCTCTGTGGCTTCTGATGTTGGCATTGTTGTTGTTGGTTTGCAAGAAGTTGAAATGGGTGCTGGTTTTCTTGCTATGTCTGCAGCCAAAGAAACTGTAGGTCTTGAGGGGAGCTCTGTTGGGCAGTGGTGGCTGGATATGATTGGAAAAACATTGGATGAAGGGTCAACCTTTGAACGTGTTGGATCCAGACAGTTGGCAGGATTGCTCATTGCTATGTGGGTGAGACATAATCTCAAAGCTCATGTCGGAGATGTCGATGCTGCAGCAGTTCCGTGTGGTTTTGGGCGCGCTATTGGTAACAAGGGAGCTGTTGGTTTGAGGATTAGATTGTATGACAGGATAATGTGCTTTGTTAATTGTCACTTTGCTGCGCATTTGGAAGCTGTTAATCGCCGCAATGCAGATTTCGATCATGTATATCGAACCATGACCTTCTCCCGACCTAATTTTCTCAATTGTGCAGCTGCTAGTGCTTCATCTGCTGTTCAAATGCTTCGCGGTACAAATGCTATTGGTAACAATTCTGTAGAAGGGATGCCGGAGTTATCTGAAGCAGACATGGTCATATTTCTTGGTGATTTTAACTATCGGCTTGATGGTATATCTTATGATGAAGCTAGAGATTTTGTTTCTCAAAGATGCTTTGATTGGCTTAGAGAACGGGATCAGCTCCGAGGGGAAATGGCAGCTGGAAATGTCTTTCAAGGAATGCGGGAGGCAAATATTACATTTCCTCCCACATACAAGTTTGAAAGGCATCAAGCTGGTTTAGCAGGGTATGATTCTGGTGAAAAGAAGCGCATTCCAGCTTGGTGTGATAGAATCCTGCATCGTGATAGCCGCTCAGCGTTGGTATCAGAATGCTGCTTAGAATGTCCTGTAGTCTCTTCAATATCACAGTACGAGGCTTCCATGGATGTAACTGACAGCGATCACAAGCCTGTCCGCTGCATTTTTACTCTTGATATTGCTCGAGTTGATGAGTCAATAAGGAGACAAGAGCTTGGAGATATCCTTGAATCAAATGGGAAACTCAAATGTATGCTTGAAGAACTATGTAGGATTCCTGAAACAATTGTCAGTACAAACAAAATAATCCTTCAAATCCAGGACACATCCATTTTGCGTATTACAAATAAATCTAGCCAAAAAGATGCTTTATTTGAAATAATTTGTGAAGGACAATCTATCATTAAGGAGGATGGACATGCATCAGATCATTGTCCCAGAGGTTCCTTTGGCTTTCCGCGATGGCTTCAGGTCACTCCTGCAGCTGGCATAATCAGACCAGATCATATAGCAGAGGTGTCTGTTCATCATGAGGAACACCAAACCCTAGAAGAGTTTGTTGATGGTGTCCCGCAGAACTGTTGGTGTGAAAACACCAGAGACAAGGAAGTTATACTGGTGGTTAAGGTACACGGGAGGTACAGTAATAACACCAAAAGCCATCGCGTCTGTGTGCGTCACTGCTGTTCAGCCAAGACAAAGCAAAGTGACCCCCCCGAGCACAACACCAGACAAACCCAAGGAACCGCTCTTCACCGTTCTAACTTCCAACACCTTAGCAGTTCCTATGACGTGGTTGACCACCTGTGGAGTATGAATAGTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

1116

Amino Acids

123.18

Weight (kDa)

5.8

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_IP5PC_F PF23754 137 - 525 1.2e-187 IP5P C-F beta-propeller
Exo_endo_phos2 PF22669 559 - 894 8.5e-66 Endonuclease/Exonuclease/phosphatase family 2
Exo_endo_phos PF03372 559 - 890 6.6e-07 Endonuclease/Exonuclease/phosphatase family
Ig-like_IP5PC_F PF23755 933 - 1069 3.3e-70 IP5P C-F Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010730)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65580 AT1G65580
fragaria_vesca FvH4_6g00030 FvH4_6g00030
malus_domestica MD12G1260800.v1.1
prunus_persica Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1
pyrus_communis pycom12g24280
rosa_chinensis RchiOBHm_Chr3g0447351
rosa_laevigata RLG00000026007
rosa_multiflora Rmu_ssc0000473.1_g000002
rosa_roxburghii Rroxscaffold_6G00425530
rosa_rugosa Rorug02G0600200 Rorug02G0600300
rosa_samantha Rh3AG000500 Rh3BG000300 Rh3CG000300 Rh3DG000500
rosa_wichuraiana Rw3G000030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2106
Acc65I GGTACC 1 cut(s) 329
AccB1I GGYRCC 3 cut(s) 329, 568, 1244
AccB7I CCANNNNNTGG 1 cut(s) 1456
AccBSI CCGCTC 2 cut(s) 2577, 3272
AccI GTMKAC 1 cut(s) 240
AccII CGCG 7 cut(s) 53, 599, 1042, 2022, 2232, 2983, 3189
AclI AACGTT 1 cut(s) 1686
AclWI GGATC 5 cut(s) 792, 905, 1911, 1924, 2406
AcoI YGGCCR 3 cut(s) 377, 824, 1548
AcsI RAATTY 4 cut(s) 502, 1130, 1367, 1634
AcuI CTGAAG 4 cut(s) 942, 1293, 2304, 2975
AcyI GRCGYC 2 cut(s) 144, 270
AfaI GTAC 9 cut(s) 331, 518, 1405, 1678, 2236, 2636, 2818, 3153, 3164
AfiI CCNNNNNNNGG 6 cut(s) 355, 356, 468, 469, 1456, 3008
AflIII ACRYGT 2 cut(s) 876, 1909
AhlI ACTAGT 1 cut(s) 700
AjiI CACGTC 1 cut(s) 3316
AjnI CCWGG 4 cut(s) 914, 1449, 1724, 2841
Alw21I GWGCWC 3 cut(s) 593, 1847, 3246
Alw26I GTCTC 9 cut(s) 33, 89, 154, 410, 858, 1951, 2623, 2722, 3117
Alw44I GTGCAC 1 cut(s) 589
AlwI GGATC 5 cut(s) 792, 905, 1911, 1924, 2406
AlwNI CAGNNNCTG 2 cut(s) 1220, 1529
Ama87I CYCGRG 2 cut(s) 2706, 3239
AoxI GGCC 7 cut(s) 315, 377, 398, 748, 824, 1324, 1548
ApaLI GTGCAC 1 cut(s) 589
ApoI RAATTY 4 cut(s) 502, 1130, 1367, 1634
ArsI GACNNNNNNTTYG 4 cut(s) 67, 99, 1962, 1994
Asp718I GGTACC 1 cut(s) 329
AspLEI GCGC 8 cut(s) 55, 488, 1042, 1044, 2022, 2024, 2107, 2535
AspS9I GGNCC 6 cut(s) 364, 399, 749, 918, 1459, 1592
AsuC2I CCSGG 2 cut(s) 350, 1590
AsuHPI GGTGA 7 cut(s) 108, 613, 1578, 1966, 2321, 2534, 3269
AvaI CYCGRG 2 cut(s) 2706, 3239
AvaII GGWCC 4 cut(s) 364, 918, 1459, 1592
BaeGI GKGCMC 1 cut(s) 593
BalI TGGCCA 2 cut(s) 826, 1550
BamHI GGATCC 1 cut(s) 1916
BanI GGYRCC 3 cut(s) 329, 568, 1244
BanII GRGCYC 3 cut(s) 293, 1736, 1847
BbsI GAAGAC 1 cut(s) 873
Bbv12I GWGCWC 3 cut(s) 593, 1847, 3246
BbvCI CCTCAGC 3 cut(s) 410, 534, 694
BceAI ACGGC 4 cut(s) 106, 131, 473, 582
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 4 cut(s) 916, 1451, 1726, 2843
BcnI CCSGG 2 cut(s) 350, 1590
BcoDI GTCTC 9 cut(s) 33, 89, 154, 410, 858, 1951, 2623, 2722, 3117
BcuI ACTAGT 1 cut(s) 700
BfaI CTAG 5 cut(s) 701, 2202, 2352, 2877, 3071
BfmI CTRYAG 7 cut(s) 1521, 1812, 1827, 1995, 2259, 2613, 3003
BglI GCCNNNNNGGC 1 cut(s) 385
BlpI GCTNAGC 1 cut(s) 2578
Bme1390I CCNGG 6 cut(s) 350, 916, 1451, 1590, 1726, 2843
Bme18I GGWCC 4 cut(s) 364, 918, 1459, 1592
BmeT110I CYCGRG 2 cut(s) 2706, 3239
BmgBI CACGTC 1 cut(s) 3316
BmgT120I GGNCC 6 cut(s) 364, 399, 749, 918, 1459, 1592
BmrFI CCNGG 6 cut(s) 350, 916, 1451, 1590, 1726, 2843
BoxI GACNNNNGTC 3 cut(s) 446, 1500, 1702
BpiI GAAGAC 1 cut(s) 873
BplI GAGNNNNNCTC 4 cut(s) 403, 435, 1829, 1861
BpmI CTGGAG 2 cut(s) 973, 1161
Bpu10I CCTNAGC 5 cut(s) 285, 410, 534, 694, 3299
Bpu1102I GCTNAGC 1 cut(s) 2578
BpuEI CTTGAG 1 cut(s) 1856
BpuMI CCSGG 2 cut(s) 350, 1590
BsaAI YACGTR 2 cut(s) 1193, 1418
BsaBI GATNNNNATC 2 cut(s) 1260, 2142
BsaHI GRCGYC 2 cut(s) 144, 270
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 9 cut(s) 318, 383, 597, 827, 914, 1545, 2407, 2646, 3262
BsaWI WCCGGW 1 cut(s) 332
Bsc4I CCNNNNNNNGG 6 cut(s) 355, 356, 468, 469, 1456, 3008
Bse118I RCCGGY 2 cut(s) 375, 523
Bse1I ACTGG 3 cut(s) 990, 1678, 3144
Bse3DI GCAATG 2 cut(s) 1941, 2137
Bse8I GATNNNNATC 2 cut(s) 1260, 2142
BseBI CCWGG 4 cut(s) 916, 1451, 1726, 2843
BseDI CCNNGG 9 cut(s) 318, 383, 597, 827, 914, 1545, 2407, 2646, 3262
BseJI GATNNNNATC 2 cut(s) 1260, 2142
BseLI CCNNNNNNNGG 6 cut(s) 355, 356, 468, 469, 1456, 3008
BseMI GCAATG 2 cut(s) 1941, 2137
BseMII CTCAG 9 cut(s) 23, 297, 299, 386, 401, 434, 525, 708, 2592
BseNI ACTGG 3 cut(s) 990, 1678, 3144
BsePI GCGCGC 2 cut(s) 1040, 2020
BseRI GAGGAG 6 cut(s) 37, 283, 286, 676, 679, 682
BseSI GKGCMC 1 cut(s) 593
BseX3I CGGCCG 1 cut(s) 377
BseYI CCCAGC 2 cut(s) 473, 1729
BsgI GTGCAG 2 cut(s) 1355, 2214
Bsh1236I CGCG 7 cut(s) 53, 599, 1042, 2022, 2232, 2983, 3189
Bsh1285I CGRYCG 2 cut(s) 202, 380
BshFI GGCC 7 cut(s) 317, 379, 400, 750, 826, 1326, 1550
BshNI GGYRCC 3 cut(s) 329, 568, 1244
BsiEI CGRYCG 2 cut(s) 202, 380
BsiHKAI GWGCWC 3 cut(s) 593, 1847, 3246
BsiHKCI CYCGRG 2 cut(s) 2706, 3239
BsiSI CCGG 6 cut(s) 333, 350, 376, 524, 1589, 2273
BslFI GGGAC 6 cut(s) 332, 445, 490, 1605, 2943, 3077
BslI CCNNNNNNNGG 6 cut(s) 355, 356, 468, 469, 1456, 3008
BsmAI GTCTC 9 cut(s) 33, 89, 154, 410, 858, 1951, 2623, 2722, 3117
BsmBI CGTCTC 2 cut(s) 33, 154
BsmFI GGGAC 6 cut(s) 332, 445, 490, 1605, 2943, 3077
BsmI GAATGC 3 cut(s) 2448, 2535, 2600
BsnI GGCC 7 cut(s) 317, 379, 400, 750, 826, 1326, 1550
Bso31I GGTCTC 1 cut(s) 89
BsoBI CYCGRG 2 cut(s) 2706, 3239
Bsp1286I GDGCHC 5 cut(s) 293, 593, 1736, 1847, 3246
Bsp143I GATC 9 cut(s) 784, 910, 1187, 1916, 2143, 2398, 2665, 2950, 3025
Bsp1720I GCTNAGC 1 cut(s) 2578
Bsp19I CCATGG 2 cut(s) 827, 2646
BspANI GGCC 7 cut(s) 317, 379, 400, 750, 826, 1326, 1550
BspCNI CTCAG 9 cut(s) 24, 298, 298, 387, 402, 433, 526, 707, 2591
BspFNI CGCG 7 cut(s) 53, 599, 1042, 2022, 2232, 2983, 3189
BspHI TCATGA 1 cut(s) 3052
BspMAI CTGCAG 3 cut(s) 1816, 1999, 3007
BspPI GGATC 5 cut(s) 792, 905, 1911, 1924, 2406
BspQI GCTCTTC 1 cut(s) 3279
BspT107I GGYRCC 3 cut(s) 329, 568, 1244
BspTNI GGTCTC 1 cut(s) 89
BsrBI CCGCTC 2 cut(s) 2577, 3272
BsrDI GCAATG 2 cut(s) 1941, 2137
BsrFI RCCGGY 2 cut(s) 375, 523
BsrI ACTGG 3 cut(s) 990, 1678, 3144
BssAI RCCGGY 2 cut(s) 375, 523
BssECI CCNNGG 9 cut(s) 318, 383, 597, 827, 914, 1545, 2407, 2646, 3262
BssHII GCGCGC 2 cut(s) 1040, 2020
BssMI GATC 9 cut(s) 784, 910, 1187, 1916, 2143, 2398, 2665, 2950, 3025
BssNI GRCGYC 2 cut(s) 144, 270
BssT1I CCWWGG 6 cut(s) 318, 383, 827, 1545, 2646, 3262
Bst2UI CCWGG 4 cut(s) 916, 1451, 1726, 2843
Bst6I CTCTTC 5 cut(s) 107, 118, 2626, 3069, 3279
BstACI GRCGYC 2 cut(s) 144, 270
BstAPI GCANNNNNTGC 1 cut(s) 1217
BstBAI YACGTR 2 cut(s) 1193, 1418
BstC8I GCNNGC 7 cut(s) 206, 525, 738, 1042, 1627, 2022, 3010
BstDSI CCRYGG 3 cut(s) 597, 827, 2646
BstFNI CGCG 7 cut(s) 53, 599, 1042, 2022, 2232, 2983, 3189
BstHHI GCGC 8 cut(s) 55, 488, 1042, 1044, 2022, 2024, 2107, 2535
BstKTI GATC 9 cut(s) 787, 913, 1190, 1919, 2146, 2401, 2668, 2953, 3028
BstMAI GTCTC 9 cut(s) 33, 89, 154, 410, 858, 1951, 2623, 2722, 3117
BstMBI GATC 9 cut(s) 784, 910, 1187, 1916, 2143, 2398, 2665, 2950, 3025
BstMCI CGRYCG 2 cut(s) 202, 380
BstNI CCWGG 4 cut(s) 916, 1451, 1726, 2843
BstNSI RCATGY 2 cut(s) 880, 2945
BstPAI GACNNNNGTC 3 cut(s) 446, 1500, 1702
BstSCI CCNGG 6 cut(s) 348, 914, 1449, 1588, 1724, 2841
BstSFI CTRYAG 7 cut(s) 1521, 1812, 1827, 1995, 2259, 2613, 3003
BstSLI GKGCMC 1 cut(s) 593
BstSNI TACGTA 1 cut(s) 1418
BstUI CGCG 7 cut(s) 53, 599, 1042, 2022, 2232, 2983, 3189
BstV2I GAAGAC 1 cut(s) 873
BstX2I RGATCY 2 cut(s) 784, 1916
BstXI CCANNNNNNTGG 2 cut(s) 80, 1927
BstYI RGATCY 2 cut(s) 784, 1916
BstZI CGGCCG 1 cut(s) 377
BsuRI GGCC 7 cut(s) 317, 379, 400, 750, 826, 1326, 1550
BtgI CCRYGG 3 cut(s) 597, 827, 2646
BtgZI GCGATG 2 cut(s) 2998, 3170
BtrI CACGTC 1 cut(s) 3316
BtsI GCAGTG 5 cut(s) 279, 1242, 1343, 1862, 3202
BtsIMutI CAGTG 9 cut(s) 279, 997, 1028, 1131, 1242, 1343, 1443, 1862, 3202
Cac8I GCNNGC 7 cut(s) 206, 525, 738, 1042, 1627, 2022, 3010
CaiI CAGNNNCTG 2 cut(s) 1220, 1529
CciI TCATGA 1 cut(s) 3052
CfoI GCGC 8 cut(s) 55, 488, 1042, 1044, 2022, 2024, 2107, 2535
Cfr10I RCCGGY 2 cut(s) 375, 523
Cfr13I GGNCC 6 cut(s) 364, 399, 749, 918, 1459, 1592
Cfr42I CCGCGG 1 cut(s) 600
CseI GACGC 5 cut(s) 152, 278, 1692, 3178, 3188
Csp6I GTAC 9 cut(s) 330, 517, 1404, 1677, 2235, 2635, 2817, 3152, 3163
CspCI CAANNNNNGTGG 2 cut(s) 1540, 1575
CviQI GTAC 9 cut(s) 330, 517, 1404, 1677, 2235, 2635, 2817, 3152, 3163
DpnI GATC 9 cut(s) 786, 912, 1189, 1918, 2145, 2400, 2667, 2952, 3027
DpnII GATC 9 cut(s) 784, 910, 1187, 1916, 2143, 2398, 2665, 2950, 3025
DraI TTTAAA 1 cut(s) 508
EaeI YGGCCR 3 cut(s) 377, 824, 1548
EagI CGGCCG 1 cut(s) 377
Eam1104I CTCTTC 5 cut(s) 107, 118, 2626, 3069, 3279
EarI CTCTTC 5 cut(s) 107, 118, 2626, 3069, 3279
EciI GGCGGA 1 cut(s) 339
Ecl136II GAGCTC 1 cut(s) 1845
EclXI CGGCCG 1 cut(s) 377
Eco105I TACGTA 1 cut(s) 1418
Eco130I CCWWGG 6 cut(s) 318, 383, 827, 1545, 2646, 3262
Eco24I GRGCYC 3 cut(s) 293, 1736, 1847
Eco31I GGTCTC 1 cut(s) 89
Eco32I GATATC 1 cut(s) 2746
Eco47I GGWCC 4 cut(s) 364, 918, 1459, 1592
Eco52I CGGCCG 1 cut(s) 377
Eco53kI GAGCTC 1 cut(s) 1845
Eco57I CTGAAG 4 cut(s) 942, 1293, 2304, 2975
Eco88I CYCGRG 2 cut(s) 2706, 3239
EcoICRI GAGCTC 1 cut(s) 1845
EcoO109I RGGNCCY 3 cut(s) 399, 749, 1592
EcoRI GAATTC 1 cut(s) 1367
EcoRII CCWGG 4 cut(s) 914, 1449, 1724, 2841
EcoRV GATATC 1 cut(s) 2746
EcoT14I CCWWGG 6 cut(s) 318, 383, 827, 1545, 2646, 3262
EcoT22I ATGCAT 1 cut(s) 2947
EcoT38I GRGCYC 3 cut(s) 293, 1736, 1847
ErhI CCWWGG 6 cut(s) 318, 383, 827, 1545, 2646, 3262
Esp3I CGTCTC 2 cut(s) 33, 154
FalI AAGNNNNNCTT 4 cut(s) 710, 742, 1232, 1264
FaqI GGGAC 6 cut(s) 332, 445, 490, 1605, 2943, 3077
FauI CCCGC 2 cut(s) 2439, 3102
FblI GTMKAC 1 cut(s) 240
FriOI GRGCYC 3 cut(s) 293, 1736, 1847
FspBI CTAG 5 cut(s) 701, 2202, 2352, 2877, 3071
FspI TGCGCA 1 cut(s) 2106
GlaI GCGC 8 cut(s) 54, 487, 1041, 1043, 2021, 2023, 2106, 2534
GsaI CCCAGC 2 cut(s) 477, 1733
GsuI CTGGAG 2 cut(s) 973, 1161
HaeIII GGCC 7 cut(s) 317, 379, 400, 750, 826, 1326, 1550
HapII CCGG 6 cut(s) 333, 350, 376, 524, 1589, 2273
HgaI GACGC 5 cut(s) 152, 278, 1692, 3178, 3188
HhaI GCGC 8 cut(s) 55, 488, 1042, 1044, 2022, 2024, 2107, 2535
Hin1I GRCGYC 2 cut(s) 144, 270
Hin6I GCGC 8 cut(s) 53, 486, 1040, 1042, 2020, 2022, 2105, 2533
HinP1I GCGC 8 cut(s) 53, 486, 1040, 1042, 2020, 2022, 2105, 2533
HincII GTYRAC 5 cut(s) 226, 232, 241, 1899, 3322
HindII GTYRAC 5 cut(s) 226, 232, 241, 1899, 3322
HpaII CCGG 6 cut(s) 333, 350, 376, 524, 1589, 2273
HphI GGTGA 7 cut(s) 108, 613, 1578, 1966, 2321, 2534, 3269
Hpy99I CGWCG 7 cut(s) 17, 92, 239, 242, 272, 1230, 1469
HpyAV CCTTC 7 cut(s) 545, 1234, 1886, 2176, 2258, 2843, 2906
HpyCH4IV ACGT 8 cut(s) 222, 228, 234, 1192, 1417, 1686, 1909, 3315
HpySE526I ACGT 8 cut(s) 222, 228, 234, 1192, 1417, 1686, 1909, 3315
Hsp92I GRCGYC 2 cut(s) 144, 270
HspAI GCGC 8 cut(s) 53, 486, 1040, 1042, 2020, 2022, 2105, 2533
KpnI GGTACC 1 cut(s) 333
KroI GCCGGC 1 cut(s) 523
KroNI GCCGGC 1 cut(s) 525
KspI CCGCGG 1 cut(s) 600
Kzo9I GATC 9 cut(s) 784, 910, 1187, 1916, 2143, 2398, 2665, 2950, 3025
LguI GCTCTTC 1 cut(s) 3279
MaeI CTAG 5 cut(s) 701, 2202, 2352, 2877, 3071
MaeII ACGT 8 cut(s) 222, 228, 234, 1192, 1417, 1686, 1909, 3315
MalI GATC 9 cut(s) 786, 912, 1189, 1918, 2145, 2400, 2667, 2952, 3027
MbiI CCGCTC 2 cut(s) 2577, 3272
MboI GATC 9 cut(s) 784, 910, 1187, 1916, 2143, 2398, 2665, 2950, 3025
MfeI CAATTG 3 cut(s) 464, 2188, 2808
MflI RGATCY 2 cut(s) 784, 1916
MhlI GDGCHC 5 cut(s) 293, 593, 1736, 1847, 3246
MlsI TGGCCA 2 cut(s) 826, 1550
MluNI TGGCCA 2 cut(s) 826, 1550
MlyI GAGTC 7 cut(s) 104, 412, 467, 590, 677, 1706, 2726
MmeI TCCRAC 5 cut(s) 653, 1669, 1894, 1960, 3316
Mox20I TGGCCA 2 cut(s) 826, 1550
Mph1103I ATGCAT 1 cut(s) 2947
MreI CGCCGGCG 1 cut(s) 523
MroNI GCCGGC 1 cut(s) 523
MscI TGGCCA 2 cut(s) 826, 1550
MslI CAYNNNNRTG 2 cut(s) 654, 1946
Msp20I TGGCCA 2 cut(s) 826, 1550
MspA1I CMGCKG 6 cut(s) 409, 599, 2198, 2423, 2682, 3008
MspI CCGG 6 cut(s) 333, 350, 376, 524, 1589, 2273
MspR9I CCNGG 6 cut(s) 350, 916, 1451, 1590, 1726, 2843
MunI CAATTG 3 cut(s) 464, 2188, 2808
Mva1269I GAATGC 3 cut(s) 2448, 2535, 2600
MvaI CCWGG 4 cut(s) 916, 1451, 1726, 2843
MvnI CGCG 7 cut(s) 53, 599, 1042, 2022, 2232, 2983, 3189
NaeI GCCGGC 1 cut(s) 525
NciI CCSGG 2 cut(s) 350, 1590
NcoI CCATGG 2 cut(s) 827, 2646
NdeII GATC 9 cut(s) 784, 910, 1187, 1916, 2143, 2398, 2665, 2950, 3025
NgoMIV GCCGGC 1 cut(s) 523
NmuCI GTSAC 5 cut(s) 1704, 2093, 2995, 3200, 3230
NsbI TGCGCA 1 cut(s) 2106
NsiI ATGCAT 1 cut(s) 2947
NspI RCATGY 2 cut(s) 880, 2945
PaeR7I CTCGAG 1 cut(s) 2706
PagI TCATGA 1 cut(s) 3052
PauI GCGCGC 2 cut(s) 1040, 2020
PciI ACATGT 1 cut(s) 876
PciSI GCTCTTC 1 cut(s) 3279
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 234, 1986
PctI GAATGC 3 cut(s) 2448, 2535, 2600
PdiI GCCGGC 1 cut(s) 525
PfeI GAWTC 6 cut(s) 557, 1090, 2516, 2556, 2753, 2797
PflFI GACNNNGTC 2 cut(s) 146, 2294
PflMI CCANNNNNTGG 1 cut(s) 1456
PfoI TCCNGGA 1 cut(s) 2841
PleI GAGTC 7 cut(s) 104, 412, 466, 589, 677, 1706, 2725
PpsI GAGTC 7 cut(s) 104, 412, 466, 589, 677, 1706, 2725
Ppu21I YACGTR 2 cut(s) 1193, 1418
PpuMI RGGWCCY 1 cut(s) 1592
PscI ACATGT 1 cut(s) 876
PshAI GACNNNNGTC 3 cut(s) 446, 1500, 1702
Psp124BI GAGCTC 1 cut(s) 1847
Psp1406I AACGTT 1 cut(s) 1686
Psp5II RGGWCCY 1 cut(s) 1592
Psp6I CCWGG 4 cut(s) 914, 1449, 1724, 2841
PspFI CCCAGC 2 cut(s) 473, 1729
PspGI CCWGG 4 cut(s) 914, 1449, 1724, 2841
PspPI GGNCC 6 cut(s) 364, 399, 749, 918, 1459, 1592
PspPPI RGGWCCY 1 cut(s) 1592
PspXI VCTCGAGB 1 cut(s) 2706
PstI CTGCAG 3 cut(s) 1816, 1999, 3007
PstNI CAGNNNCTG 2 cut(s) 1220, 1529
PsuI RGATCY 2 cut(s) 784, 1916
PsyI GACNNNGTC 2 cut(s) 146, 2294
PteI GCGCGC 2 cut(s) 1040, 2020
PvuII CAGCTG 3 cut(s) 2198, 2423, 3008
RsaI GTAC 9 cut(s) 331, 518, 1405, 1678, 2236, 2636, 2818, 3153, 3164
RsaNI GTAC 9 cut(s) 330, 517, 1404, 1677, 2235, 2635, 2817, 3152, 3163
RseI CAYNNNNRTG 2 cut(s) 654, 1946
SacI GAGCTC 1 cut(s) 1847
SacII CCGCGG 1 cut(s) 600
SalI GTCGAC 1 cut(s) 239
SapI GCTCTTC 1 cut(s) 3279
Sau3AI GATC 9 cut(s) 784, 910, 1187, 1916, 2143, 2398, 2665, 2950, 3025
Sau96I GGNCC 6 cut(s) 364, 399, 749, 918, 1459, 1592
SchI GAGTC 7 cut(s) 104, 412, 467, 590, 677, 1706, 2726
ScrFI CCNGG 6 cut(s) 350, 916, 1451, 1590, 1726, 2843
SduI GDGCHC 5 cut(s) 293, 593, 1736, 1847, 3246
SfcI CTRYAG 7 cut(s) 1521, 1812, 1827, 1995, 2259, 2613, 3003
Sfr274I CTCGAG 1 cut(s) 2706
Sfr303I CCGCGG 1 cut(s) 600
SgrAI CRCCGGYG 1 cut(s) 523
SgrBI CCGCGG 1 cut(s) 600
SinI GGWCC 4 cut(s) 364, 918, 1459, 1592
SlaI CTCGAG 1 cut(s) 2706
SmiMI CAYNNNNRTG 2 cut(s) 654, 1946
SmlI CTYRAG 2 cut(s) 1835, 2706
SmoI CTYRAG 2 cut(s) 1835, 2706
SnaBI TACGTA 1 cut(s) 1418
SpeI ACTAGT 1 cut(s) 700
SspI AATATT 3 cut(s) 1579, 1668, 2458
SspMI CTAG 5 cut(s) 701, 2202, 2352, 2877, 3071
SstI GAGCTC 1 cut(s) 1847
StyD4I CCNGG 6 cut(s) 348, 914, 1449, 1588, 1724, 2841
StyI CCWWGG 6 cut(s) 318, 383, 827, 1545, 2646, 3262
TaiI ACGT 8 cut(s) 225, 231, 237, 1195, 1420, 1689, 1912, 3318
TatI WGTACW 3 cut(s) 516, 1403, 2816
TauI GCSGC 7 cut(s) 96, 275, 382, 530, 533, 2130, 2577
TfiI GAWTC 6 cut(s) 557, 1090, 2516, 2556, 2753, 2797
TscAI CASTG 9 cut(s) 286, 997, 1035, 1131, 1242, 1343, 1450, 1862, 3209
TseFI GTSAC 5 cut(s) 1704, 2093, 2995, 3200, 3230
Tsp45I GTSAC 5 cut(s) 1704, 2093, 2995, 3200, 3230
TspDTI ATGAA 7 cut(s) 114, 150, 1095, 1905, 2199, 2361, 3038
TspGWI ACGGA 5 cut(s) 29, 202, 494, 574, 1995
TspRI CASTG 9 cut(s) 286, 997, 1035, 1131, 1242, 1343, 1450, 1862, 3209
Tth111I GACNNNGTC 2 cut(s) 146, 2294
Van91I CCANNNNNTGG 1 cut(s) 1456
VneI GTGCAC 1 cut(s) 589
VpaK11BI GGWCC 4 cut(s) 364, 918, 1459, 1592
XapI RAATTY 4 cut(s) 502, 1130, 1367, 1634
XceI RCATGY 2 cut(s) 880, 2945
XcmI CCANNNNNNNNNTGG 1 cut(s) 470
XhoI CTCGAG 1 cut(s) 2706
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 5 cut(s) 701, 2202, 2352, 2877, 3071
Zsp2I ATGCAT 1 cut(s) 2947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.