FvH4_6g03981

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007420
Physical Location & Seq
Forward (+)
2162261 .. 2171267
9007 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g03981.t1

Sequence Viewer

Length: 2283 bp
ATGCACCGAGTGGGTAGTGCTGGGAACACATCCAATTCTACTCGCCCTCGTAAAGAGAAGAGATTAACTTATGTGTTGAGTGATGCTGATGACAGAAAGCATTGCGCAGGCATAAATTGTTTGGCTGTATTGAAGGCATCAGAGGCTGGGTCTGATTACCTATTTACTGGAAGCCGCGATGGAACACTAAAAAGATGGGCTGTGGCAGAAGATGCTGCTACCTGCTCAGCAACCTTCGAGTCTCATGTGGATTGGGTTAATGATGCTGTTCTTGCAGGGGATAATACACTTGTTTCCTGTTCTTCAGATACCACCCTCAAGACGTGGGATTGCTTGTCAGATGGAACTTGTACCAGAACTCTTCGTCAGCATTCTGACTACGTCACTTGTCTTGCTGCCGCAGATAAAAATAGCAATATTGTTGCCTCTGGTGGACTTGGAGGGGAGGTATTCATTTGGGATCTCGAAGCAGCCCTTACTCCGGTCTCAAAGTCGGGTGATTCTATGGAAGATGATAGTTCAAATGGCACCAATGGGTCTGGAAATGCACAACCGACTACAAGTTTACGTACTATCAGCTCTAGCAACAGCATTTCTATGCACACAACTCCAGCACATGGATATGTTCCAATCTCTGCCAAAGGCCATAAGGAATCAGTTTATGCATTGGCAATGAATGATAGTGGATCACTTCTTGTCTCTGGTGGAACGGAGAAGGTCGTGCGTGTTTGGGACCCAAGAAGTGGCTCAAAGACTATGAAACTAAGAGGGCATACCGATAATATCCGGGCTCTGCTTCTTGATTCTACAGGCAGGTTTTGCTTATCAGGATCTTCTGATTCAATGATTAGACTATGGGATCTTGGTCAGCAAAGATGCGTACATTCTTATGCGGTGCACACAGACTCTGTTTGGGCACTTGCCAGTACGCCAACGTTTAGTCATGTATATAGTGGTGGGAGAGACCTTTCTTTGTACCTGACAGACTTGGCCACAAGAGAGAGTCTTTTGCTTTGCACAGGAGAACACCCCATTCTGCAGTTGGCTTTAGATGATGATAGCATATGGGTTGCAACAACAGATTCTTCAGTTAATAGATGGCCTGCTGAGGGGCGCAATCCTCAGAAAATTATCCAAAGAGGCGGTGCTTTCTTGGCCGGAAATTTGTCATTCTCAAGAGCAAGGGTGTCCTTAGAAGGATCTACCCTAGTCCCTGTTTATAAAGAGCCAGTCTTTACAATTCCTGGAACTCCTGGAATAGTTCAACATGAAATATTAAATAATAGACGTCATGTCTTGACAAAGGATACTGCTTCCTCAGTGAAGCTGTGGGAGATTACTAGAGGTGTTATTATTGAGGATTTCGGAAAGGTGTCTTTTGAGGAGAAAAAGGAAGAGCTTTTTGAGACTGTAAGCATTCCTGCATGGTTCACTGTGGATACCAGGCTTGGAAGTTTATCTGTACATATGGATACCCCACAATGCTTTTCAGCAGAGATGTACTCTGCAGACCTTAATATTGTAGGAAAGCCTGAAGATGACAAGGTTAATCTAGCACGAGAAACTCTTAAAGGGCTCTTGGCTCATTGGTTGGGGAAAAGAAAGCAAAGATCAGGAACACAAGCCTCAACGAACGGGGATGTGTCATTGGGAAAGGAAATTACTCCTAGGAGTATTACCCAATCAAGACTTGAGGTGGATAGTAATACCGAAAATGATTCAATGGTCTATCCTCCCTTTGAATTTTCTACAACATCCCCACCTTCCATCATCACTGAGGGCTCTCAGGGAGGACCATGGCGAAAGAAGATCACTGACCTAGATGGAACTGAAGATGAGAAGGACTTTCCATGGTGGGTTCTGGATTGTGTGTTGAACAATCGCCTACCTCCGAGAGAAAATACCAAGTGCAGTTTCTATCTACATCCTTGTGAAGGTTCAACTGTTCAGATCCTCACACAAGGAAAACTAAGTGCACCTCGTATATTACGAATTCATAAGGTGGTTAATTATGTAGTTGAAAAGATGGTCCTGGACAAACCATTAGATAGTGCAAATCCTGATGCAACATTTGCTCCTGGTCTTGCTGGAGGGCCACTGTCTGCCATTGGAGATGGATCTTTTCGGTCTGGAATGAAGCCATGGAAGCTTAAACCTTCTATAGAGATATTGTGCAACAATCAGGTCTTATCGCCAGAAATGAGCTTAGCCACAGTGCGGGCTTATGTATGGAAGAAATCCGAGGACCTGGTTCTAAATTATAGGGTGGTTCAGGGCAGGTGA

Protein Analysis

761

Amino Acids

82.93

Weight (kDa)

6.17

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 26 - 157 1.4e-07 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 27 - 112 4.5e-07 TEP-1 second beta-propeller
WD40_Prp19 PF24814 36 - 160 2.5e-11 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 42 - 157 8.2e-18 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 45 - 155 9e-13 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 45 - 161 7.4e-08 CDC20/Fizzy WD40 domain
WDR55 PF24796 49 - 153 3.5e-07 WDR55
Beta-prop_THOC3 PF25174 52 - 158 1.5e-11 THOC3 beta-propeller domain
Beta-prop_EML PF23409 98 - 276 9.4e-08 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 115 - 154 6.7e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 120 - 265 6.8e-13 CDC20/Fizzy WD40 domain
WDR55 PF24796 188 - 292 4.9e-10 WDR55
WD40_WDHD1_1st PF24817 189 - 300 3.1e-13 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 195 - 325 9.8e-27 WDR3 first beta-propeller domain
EIF3I PF24805 210 - 301 1.3e-10 EIF3I
Beta-prop_WDR3_2nd PF25172 212 - 287 1.2e-09 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 214 - 346 4.5e-08 TEP-1 second beta-propeller
WD40_Gbeta PF25391 214 - 341 3.8e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 214 - 367 4.8e-20 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 214 - 367 5.5e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 214 - 297 3.9e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR41 PF25178 214 - 330 8e-06 WDR41
Beta-prop_THOC3 PF25174 216 - 369 9.1e-21 THOC3 beta-propeller domain
WD40_Prp19 PF24814 217 - 342 1.8e-18 Prp19 WD40 domain
WD40 PF00400 251 - 287 4.8e-07 WD domain, G-beta repeat
DUF3337 PF11816 612 - 755 1.1e-30 Domain of unknown function (DUF3337)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1221
AarI CACCTGC 1 cut(s) 2268
AatII GACGTC 1 cut(s) 1291
Acc16I TGCGCA 1 cut(s) 106
Acc36I ACCTGC 3 cut(s) 230, 804, 2268
AccB1I GGYRCC 1 cut(s) 527
AccB7I CCANNNNNTGG 2 cut(s) 617, 743
AccII CGCG 1 cut(s) 177
AciI CCGC 5 cut(s) 175, 399, 893, 1143, 2218
AclI AACGTT 1 cut(s) 935
AclWI GGATC 7 cut(s) 468, 694, 838, 867, 1207, 1945, 2125
AcoI YGGCCR 2 cut(s) 990, 1155
AcsI RAATTY 3 cut(s) 1162, 1742, 1992
AcuI CTGAAG 4 cut(s) 288, 1071, 1554, 1851
AcyI GRCGYC 1 cut(s) 1288
AdeI CACNNNGTG 1 cut(s) 10
AfaI GTAC 7 cut(s) 352, 571, 882, 928, 977, 1464, 1502
AfiI CCNNNNNNNGG 6 cut(s) 481, 617, 743, 1109, 1934, 2217
AgsI TTSAA 9 cut(s) 133, 522, 843, 1265, 1722, 1742, 1876, 1941, 2021
AhdI GACNNNNNGTC 1 cut(s) 1292
AjiI CACGTC 1 cut(s) 324
AjnI CCWGG 6 cut(s) 1243, 1252, 1442, 2031, 2077, 2247
AjuI GAANNNNNNNTTGG 2 cut(s) 1898, 1930
AluBI AGCT 5 cut(s) 579, 1327, 1399, 2149, 2205
AluI AGCT 5 cut(s) 579, 1327, 1399, 2149, 2205
Alw21I GWGCWC 2 cut(s) 900, 1978
Alw26I GTCTC 5 cut(s) 246, 490, 703, 957, 1400
Alw44I GTGCAC 2 cut(s) 896, 1974
AlwI GGATC 7 cut(s) 468, 694, 838, 867, 1207, 1945, 2125
AlwNI CAGNNNCTG 2 cut(s) 146, 908
AoxI GGCC 5 cut(s) 643, 990, 1100, 1155, 2093
ApaLI GTGCAC 2 cut(s) 896, 1974
ApeKI GCWGC 3 cut(s) 215, 395, 470
ApoI RAATTY 3 cut(s) 1162, 1742, 1992
AspA2I CCTAGG 1 cut(s) 1667
AspLEI GCGC 2 cut(s) 107, 1116
AspS9I GGNCC 5 cut(s) 733, 1793, 2029, 2093, 2245
AsuC2I CCSGG 1 cut(s) 788
AsuHPI GGTGA 1 cut(s) 509
AvaII GGWCC 4 cut(s) 733, 1793, 2029, 2245
AvrII CCTAGG 1 cut(s) 1667
BaeGI GKGCMC 3 cut(s) 900, 919, 1978
BalI TGGCCA 1 cut(s) 992
BanI GGYRCC 1 cut(s) 527
BanII GRGCYC 3 cut(s) 793, 1578, 1784
BauI CACGAG 1 cut(s) 1557
Bbv12I GWGCWC 2 cut(s) 900, 1978
BbvCI CCTCAGC 1 cut(s) 1107
BbvI GCAGC 3 cut(s) 202, 382, 482
BccI CCATC 8 cut(s) 173, 189, 335, 1092, 1775, 1817, 2020, 2108
BciT130I CCWGG 6 cut(s) 1245, 1254, 1444, 2033, 2079, 2249
BciVI GTATCC 3 cut(s) 1300, 1432, 1465
BcnI CCSGG 1 cut(s) 788
BcoDI GTCTC 5 cut(s) 246, 490, 703, 957, 1400
BfaI CTAG 6 cut(s) 582, 1208, 1341, 1553, 1668, 1820
BfmI CTRYAG 4 cut(s) 807, 1037, 1506, 2160
BfuAI ACCTGC 3 cut(s) 230, 804, 2268
BfuI GTATCC 3 cut(s) 1300, 1432, 1465
BisI GCNGC 5 cut(s) 175, 216, 396, 399, 471
BlnI CCTAGG 1 cut(s) 1667
BlpI GCTNAGC 2 cut(s) 226, 2206
BlsI GCNGC 5 cut(s) 176, 217, 397, 400, 472
Bme1390I CCNGG 7 cut(s) 788, 1245, 1254, 1444, 2033, 2079, 2249
Bme18I GGWCC 4 cut(s) 733, 1793, 2029, 2245
BmeRI GACNNNNNGTC 1 cut(s) 1292
BmgBI CACGTC 1 cut(s) 324
BmgT120I GGNCC 5 cut(s) 733, 1793, 2029, 2093, 2245
BmiI GGNNCC 3 cut(s) 529, 734, 735
BmrFI CCNGG 7 cut(s) 788, 1245, 1254, 1444, 2033, 2079, 2249
BmsI GCATC 6 cut(s) 73, 146, 202, 253, 866, 2053
BplI GAGNNNNNCTC 2 cut(s) 1487, 1519
BpmI CTGGAG 2 cut(s) 594, 2109
Bpu10I CCTNAGC 1 cut(s) 1107
Bpu1102I GCTNAGC 2 cut(s) 226, 2206
BpuEI CTTGAG 3 cut(s) 302, 1159, 1712
BpuMI CCSGG 1 cut(s) 788
BsaAI YACGTR 1 cut(s) 569
BsaHI GRCGYC 1 cut(s) 1288
BsaI GGTCTC 2 cut(s) 490, 957
BsaJI CCNNGG 5 cut(s) 1667, 1796, 1850, 2141, 2241
BsaWI WCCGGW 1 cut(s) 481
BsaXI ACNNNNNCTCC 4 cut(s) 432, 462, 2059, 2089
Bsc4I CCNNNNNNNGG 6 cut(s) 481, 617, 743, 1109, 1934, 2217
Bse1I ACTGG 3 cut(s) 172, 924, 1229
Bse3DI GCAATG 2 cut(s) 100, 678
BseBI CCWGG 6 cut(s) 1245, 1254, 1444, 2033, 2079, 2249
BseDI CCNNGG 5 cut(s) 1667, 1796, 1850, 2141, 2241
BseGI GGATG 4 cut(s) 29, 1645, 1754, 1924
BseLI CCNNNNNNNGG 6 cut(s) 481, 617, 743, 1109, 1934, 2217
BseMI GCAATG 2 cut(s) 100, 678
BseMII CTCAG 6 cut(s) 240, 1098, 1136, 1332, 1767, 1799
BseNI ACTGG 3 cut(s) 172, 924, 1229
BseRI GAGGAG 1 cut(s) 1397
BseSI GKGCMC 3 cut(s) 900, 919, 1978
BseXI GCAGC 3 cut(s) 202, 382, 482
BseYI CCCAGC 2 cut(s) 20, 146
BsgI GTGCAG 1 cut(s) 1930
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 5 cut(s) 645, 992, 1102, 1157, 2095
BshNI GGYRCC 1 cut(s) 527
BsiHKAI GWGCWC 2 cut(s) 900, 1978
BsiSI CCGG 3 cut(s) 482, 787, 1158
BslFI GGGAC 2 cut(s) 746, 1196
BslI CCNNNNNNNGG 6 cut(s) 481, 617, 743, 1109, 1934, 2217
BsmAI GTCTC 5 cut(s) 246, 490, 703, 957, 1400
BsmFI GGGAC 2 cut(s) 746, 1196
BsmI GAATGC 2 cut(s) 370, 1416
BsnI GGCC 5 cut(s) 645, 992, 1102, 1157, 2095
Bso31I GGTCTC 2 cut(s) 490, 957
Bsp1286I GDGCHC 6 cut(s) 793, 900, 919, 1578, 1784, 1978
Bsp1407I TGTACA 1 cut(s) 1462
Bsp143I GATC 9 cut(s) 460, 686, 830, 859, 1199, 1610, 1809, 1950, 2117
Bsp1720I GCTNAGC 2 cut(s) 226, 2206
Bsp19I CCATGG 3 cut(s) 1796, 1850, 2141
BspACI CCGC 5 cut(s) 175, 399, 893, 1143, 2218
BspANI GGCC 5 cut(s) 645, 992, 1102, 1157, 2095
BspCNI CTCAG 6 cut(s) 239, 1099, 1135, 1331, 1768, 1798
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 3 cut(s) 529, 734, 735
BspMAI CTGCAG 2 cut(s) 1041, 1510
BspMI ACCTGC 3 cut(s) 230, 804, 2268
BspPI GGATC 7 cut(s) 468, 694, 838, 867, 1207, 1945, 2125
BspQI GCTCTTC 1 cut(s) 1389
BspT107I GGYRCC 1 cut(s) 527
BspTNI GGTCTC 2 cut(s) 490, 957
BsrDI GCAATG 2 cut(s) 100, 678
BsrGI TGTACA 1 cut(s) 1462
BsrI ACTGG 3 cut(s) 172, 924, 1229
BssECI CCNNGG 5 cut(s) 1667, 1796, 1850, 2141, 2241
BssMI GATC 9 cut(s) 460, 686, 830, 859, 1199, 1610, 1809, 1950, 2117
BssNI GRCGYC 1 cut(s) 1288
BssSI CACGAG 1 cut(s) 1557
BssT1I CCWWGG 4 cut(s) 1667, 1796, 1850, 2141
Bst2BI CACGAG 1 cut(s) 1557
Bst2UI CCWGG 6 cut(s) 1245, 1254, 1444, 2033, 2079, 2249
Bst4CI ACNGT 5 cut(s) 1411, 1435, 1945, 2100, 2215
Bst6I CTCTTC 3 cut(s) 53, 366, 1389
BstACI GRCGYC 1 cut(s) 1288
BstAPI GCANNNNNTGC 3 cut(s) 212, 819, 2072
BstAUI TGTACA 1 cut(s) 1462
BstBAI YACGTR 1 cut(s) 569
BstC8I GCNNGC 3 cut(s) 109, 1104, 2220
BstDSI CCRYGG 3 cut(s) 1796, 1850, 2141
BstENI CCTNNNNNAGG 1 cut(s) 1932
BstF5I GGATG 4 cut(s) 29, 1645, 1754, 1924
BstFNI CGCG 1 cut(s) 177
BstHHI GCGC 2 cut(s) 107, 1116
BstKTI GATC 9 cut(s) 463, 689, 833, 862, 1202, 1613, 1812, 1953, 2120
BstMAI GTCTC 5 cut(s) 246, 490, 703, 957, 1400
BstMBI GATC 9 cut(s) 460, 686, 830, 859, 1199, 1610, 1809, 1950, 2117
BstMWI GCNNNNNNNGC 7 cut(s) 143, 212, 272, 819, 1154, 2072, 2146
BstNI CCWGG 6 cut(s) 1245, 1254, 1444, 2033, 2079, 2249
BstSCI CCNGG 7 cut(s) 786, 1243, 1252, 1442, 2031, 2077, 2247
BstSFI CTRYAG 4 cut(s) 807, 1037, 1506, 2160
BstSLI GKGCMC 3 cut(s) 900, 919, 1978
BstSNI TACGTA 1 cut(s) 569
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 3 cut(s) 202, 382, 482
BstX2I RGATCY 6 cut(s) 460, 830, 859, 1199, 1950, 2117
BstYI RGATCY 6 cut(s) 460, 830, 859, 1199, 1950, 2117
BsuI GTATCC 3 cut(s) 1300, 1432, 1465
BsuRI GGCC 5 cut(s) 645, 992, 1102, 1157, 2095
BtgI CCRYGG 3 cut(s) 1796, 1850, 2141
BtgZI GCGATG 1 cut(s) 192
BtrI CACGTC 1 cut(s) 324
BtsCI GGATG 4 cut(s) 29, 1645, 1754, 1924
BtsIMutI CAGTG 6 cut(s) 1326, 1431, 1773, 1812, 2096, 2220
BveI ACCTGC 3 cut(s) 230, 804, 2268
Cac8I GCNNGC 3 cut(s) 109, 1104, 2220
CaiI CAGNNNCTG 2 cut(s) 146, 908
CfoI GCGC 2 cut(s) 107, 1116
Cfr13I GGNCC 5 cut(s) 733, 1793, 2029, 2093, 2245
CsiI ACCWGGT 1 cut(s) 2247
Csp6I GTAC 7 cut(s) 351, 570, 881, 927, 976, 1463, 1501
CviAII CATG 9 cut(s) 245, 617, 944, 1268, 1292, 1425, 1797, 1851, 2142
CviQI GTAC 7 cut(s) 351, 570, 881, 927, 976, 1463, 1501
DpnI GATC 9 cut(s) 462, 688, 832, 861, 1201, 1612, 1811, 1952, 2119
DpnII GATC 9 cut(s) 460, 686, 830, 859, 1199, 1610, 1809, 1950, 2117
DraIII CACNNNGTG 1 cut(s) 10
DriI GACNNNNNGTC 1 cut(s) 1292
EaeI YGGCCR 2 cut(s) 990, 1155
Eam1104I CTCTTC 3 cut(s) 53, 366, 1389
Eam1105I GACNNNNNGTC 1 cut(s) 1292
EarI CTCTTC 3 cut(s) 53, 366, 1389
Eco105I TACGTA 1 cut(s) 569
Eco130I CCWWGG 4 cut(s) 1667, 1796, 1850, 2141
Eco24I GRGCYC 3 cut(s) 793, 1578, 1784
Eco31I GGTCTC 2 cut(s) 490, 957
Eco47I GGWCC 4 cut(s) 733, 1793, 2029, 2245
Eco57I CTGAAG 4 cut(s) 288, 1071, 1554, 1851
EcoNI CCTNNNNNAGG 1 cut(s) 1932
EcoO109I RGGNCCY 2 cut(s) 733, 2245
EcoRI GAATTC 1 cut(s) 1992
EcoRII CCWGG 6 cut(s) 1243, 1252, 1442, 2031, 2077, 2247
EcoT14I CCWWGG 4 cut(s) 1667, 1796, 1850, 2141
EcoT22I ATGCAT 1 cut(s) 667
EcoT38I GRGCYC 3 cut(s) 793, 1578, 1784
ErhI CCWWGG 4 cut(s) 1667, 1796, 1850, 2141
FaeI CATG 9 cut(s) 248, 620, 947, 1271, 1295, 1428, 1800, 1854, 2145
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FaqI GGGAC 2 cut(s) 746, 1196
FatI CATG 9 cut(s) 244, 616, 943, 1267, 1291, 1424, 1796, 1850, 2141
FauI CCCGC 1 cut(s) 2211
FauNDI CATATG 2 cut(s) 1064, 1467
Fnu4HI GCNGC 5 cut(s) 175, 216, 396, 399, 471
FokI GGATG 4 cut(s) 16, 1652, 1741, 1911
FriOI GRGCYC 3 cut(s) 793, 1578, 1784
Fsp4HI GCNGC 5 cut(s) 175, 216, 396, 399, 471
FspBI CTAG 6 cut(s) 582, 1208, 1341, 1553, 1668, 1820
FspI TGCGCA 1 cut(s) 106
GlaI GCGC 2 cut(s) 106, 1115
GluI GCNGC 5 cut(s) 175, 216, 396, 399, 471
GsaI CCCAGC 2 cut(s) 24, 150
GsuI CTGGAG 2 cut(s) 594, 2109
HaeIII GGCC 5 cut(s) 645, 992, 1102, 1157, 2095
HapII CCGG 3 cut(s) 482, 787, 1158
HhaI GCGC 2 cut(s) 107, 1116
Hin1I GRCGYC 1 cut(s) 1288
Hin1II CATG 9 cut(s) 248, 620, 947, 1271, 1295, 1428, 1800, 1854, 2145
Hin6I GCGC 2 cut(s) 105, 1114
HinP1I GCGC 2 cut(s) 105, 1114
HindIII AAGCTT 1 cut(s) 2147
HinfI GANTC 9 cut(s) 239, 500, 653, 803, 839, 905, 1003, 1082, 1718
HpaII CCGG 3 cut(s) 482, 787, 1158
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 5 cut(s) 434, 566, 898, 1431, 1976
Hpy8I GTNNAC 5 cut(s) 434, 566, 898, 1431, 1976
HpyAV CCTTC 8 cut(s) 127, 244, 709, 1190, 1773, 1834, 1928, 2166
HpyCH4III ACNGT 5 cut(s) 1411, 1435, 1945, 2100, 2215
HpyCH4IV ACGT 5 cut(s) 323, 381, 568, 935, 1288
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 212, 272, 819, 1154, 2072, 2146
HpySE526I ACGT 5 cut(s) 323, 381, 568, 935, 1288
Hsp92I GRCGYC 1 cut(s) 1288
Hsp92II CATG 9 cut(s) 248, 620, 947, 1271, 1295, 1428, 1800, 1854, 2145
HspAI GCGC 2 cut(s) 105, 1114
KflI GGGWCCC 1 cut(s) 733
Kzo9I GATC 9 cut(s) 460, 686, 830, 859, 1199, 1610, 1809, 1950, 2117
LguI GCTCTTC 1 cut(s) 1389
LmnI GCTCC 1 cut(s) 2080
Lsp1109I GCAGC 3 cut(s) 202, 382, 482
LweI GCATC 6 cut(s) 73, 146, 202, 253, 866, 2053
MabI ACCWGGT 1 cut(s) 2247
MaeI CTAG 6 cut(s) 582, 1208, 1341, 1553, 1668, 1820
MaeII ACGT 5 cut(s) 323, 381, 568, 935, 1288
MaeIII GTNAC 1 cut(s) 382
MalI GATC 9 cut(s) 462, 688, 832, 861, 1201, 1612, 1811, 1952, 2119
MboI GATC 9 cut(s) 460, 686, 830, 859, 1199, 1610, 1809, 1950, 2117
MflI RGATCY 6 cut(s) 460, 830, 859, 1199, 1950, 2117
MhlI GDGCHC 6 cut(s) 793, 900, 919, 1578, 1784, 1978
MlsI TGGCCA 1 cut(s) 992
MluNI TGGCCA 1 cut(s) 992
MlyI GAGTC 3 cut(s) 248, 899, 1012
Mox20I TGGCCA 1 cut(s) 992
Mph1103I ATGCAT 1 cut(s) 667
MscI TGGCCA 1 cut(s) 992
MseI TTAA 9 cut(s) 65, 258, 1092, 1277, 1515, 1548, 1569, 2007, 2151
MslI CAYNNNNRTG 4 cut(s) 596, 621, 888, 1929
Msp20I TGGCCA 1 cut(s) 992
MspI CCGG 3 cut(s) 482, 787, 1158
MspR9I CCNGG 7 cut(s) 788, 1245, 1254, 1444, 2033, 2079, 2249
Mva1269I GAATGC 2 cut(s) 370, 1416
MvaI CCWGG 6 cut(s) 1245, 1254, 1444, 2033, 2079, 2249
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 7 cut(s) 143, 212, 272, 819, 1154, 2072, 2146
NciI CCSGG 1 cut(s) 788
NcoI CCATGG 3 cut(s) 1796, 1850, 2141
NdeI CATATG 2 cut(s) 1064, 1467
NdeII GATC 9 cut(s) 460, 686, 830, 859, 1199, 1610, 1809, 1950, 2117
NlaIII CATG 9 cut(s) 248, 620, 947, 1271, 1295, 1428, 1800, 1854, 2145
NlaIV GGNNCC 3 cut(s) 529, 734, 735
NmuCI GTSAC 1 cut(s) 382
NsbI TGCGCA 1 cut(s) 106
NsiI ATGCAT 1 cut(s) 667
PaqCI CACCTGC 1 cut(s) 2268
PciSI GCTCTTC 1 cut(s) 1389
PcsI WCGNNNNNNNCGW 1 cut(s) 1987
PctI GAATGC 2 cut(s) 370, 1416
PfeI GAWTC 6 cut(s) 500, 653, 803, 839, 1082, 1718
PflFI GACNNNGTC 1 cut(s) 380
PflMI CCANNNNNTGG 2 cut(s) 617, 743
PfoI TCCNGGA 3 cut(s) 1243, 1252, 2031
PkrI GCNGC 5 cut(s) 176, 217, 397, 400, 472
PleI GAGTC 3 cut(s) 247, 899, 1011
PpsI GAGTC 3 cut(s) 247, 899, 1011
Ppu21I YACGTR 1 cut(s) 569
PpuMI RGGWCCY 2 cut(s) 733, 2245
PsiI TTATAA 1 cut(s) 1221
Psp1406I AACGTT 1 cut(s) 935
Psp5II RGGWCCY 2 cut(s) 733, 2245
Psp6I CCWGG 6 cut(s) 1243, 1252, 1442, 2031, 2077, 2247
PspFI CCCAGC 2 cut(s) 20, 146
PspGI CCWGG 6 cut(s) 1243, 1252, 1442, 2031, 2077, 2247
PspN4I GGNNCC 3 cut(s) 529, 734, 735
PspPI GGNCC 5 cut(s) 733, 1793, 2029, 2093, 2245
PspPPI RGGWCCY 2 cut(s) 733, 2245
PstI CTGCAG 2 cut(s) 1041, 1510
PstNI CAGNNNCTG 2 cut(s) 146, 908
PsuI RGATCY 6 cut(s) 460, 830, 859, 1199, 1950, 2117
PsyI GACNNNGTC 1 cut(s) 380
RsaI GTAC 7 cut(s) 352, 571, 882, 928, 977, 1464, 1502
RsaNI GTAC 7 cut(s) 351, 570, 881, 927, 976, 1463, 1501
RseI CAYNNNNRTG 4 cut(s) 596, 621, 888, 1929
SapI GCTCTTC 1 cut(s) 1389
SaqAI TTAA 9 cut(s) 65, 258, 1092, 1277, 1515, 1548, 1569, 2007, 2151
SatI GCNGC 5 cut(s) 175, 216, 396, 399, 471
Sau3AI GATC 9 cut(s) 460, 686, 830, 859, 1199, 1610, 1809, 1950, 2117
Sau96I GGNCC 5 cut(s) 733, 1793, 2029, 2093, 2245
SchI GAGTC 3 cut(s) 248, 899, 1012
ScrFI CCNGG 7 cut(s) 788, 1245, 1254, 1444, 2033, 2079, 2249
SduI GDGCHC 6 cut(s) 793, 900, 919, 1578, 1784, 1978
SexAI ACCWGGT 1 cut(s) 2247
SfaNI GCATC 6 cut(s) 73, 146, 202, 253, 866, 2053
SfcI CTRYAG 4 cut(s) 807, 1037, 1506, 2160
SinI GGWCC 4 cut(s) 733, 1793, 2029, 2245
SmiMI CAYNNNNRTG 4 cut(s) 596, 621, 888, 1929
SmlI CTYRAG 3 cut(s) 317, 1174, 1691
SmoI CTYRAG 3 cut(s) 317, 1174, 1691
SnaBI TACGTA 1 cut(s) 569
SsiI CCGC 5 cut(s) 175, 399, 893, 1143, 2218
SspI AATATT 3 cut(s) 418, 1275, 1519
SspMI CTAG 6 cut(s) 582, 1208, 1341, 1553, 1668, 1820
StyD4I CCNGG 7 cut(s) 786, 1243, 1252, 1442, 2031, 2077, 2247
StyI CCWWGG 4 cut(s) 1667, 1796, 1850, 2141
TaaI ACNGT 5 cut(s) 1411, 1435, 1945, 2100, 2215
TaiI ACGT 5 cut(s) 326, 384, 571, 938, 1291
TaqI TCGA 2 cut(s) 237, 465
TaqII GACCGA 1 cut(s) 2115
TatI WGTACW 2 cut(s) 1462, 1500
TauI GCSGC 2 cut(s) 177, 401
TfiI GAWTC 6 cut(s) 500, 653, 803, 839, 1082, 1718
Tru1I TTAA 9 cut(s) 65, 258, 1092, 1277, 1515, 1548, 1569, 2007, 2151
Tru9I TTAA 9 cut(s) 65, 258, 1092, 1277, 1515, 1548, 1569, 2007, 2151
TscAI CASTG 6 cut(s) 1326, 1438, 1780, 1819, 2103, 2220
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 3 cut(s) 215, 395, 470
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 6 cut(s) 442, 689, 773, 1284, 1985, 2150
TspGWI ACGGA 1 cut(s) 725
TspRI CASTG 6 cut(s) 1326, 1438, 1780, 1819, 2103, 2220
Tth111I GACNNNGTC 1 cut(s) 380
Van91I CCANNNNNTGG 2 cut(s) 617, 743
VneI GTGCAC 2 cut(s) 896, 1974
VpaK11BI GGWCC 4 cut(s) 733, 1793, 2029, 2245
XagI CCTNNNNNAGG 1 cut(s) 1932
XapI RAATTY 3 cut(s) 1162, 1742, 1992
XcmI CCANNNNNNNNNTGG 1 cut(s) 1039
XmaJI CCTAGG 1 cut(s) 1667
XspI CTAG 6 cut(s) 582, 1208, 1341, 1553, 1668, 1820
ZraI GACGTC 1 cut(s) 1289
Zsp2I ATGCAT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.