Rh3BG046000

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0007420
Physical Location & Seq
Forward (+)
2975969 .. 2985474
9506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG046000.1

Sequence Viewer

Length: 2067 bp
ATGCACCGAGTGGGTAGTGCTGGGAACACATCCAATTCTAGTCGCCCTCGTAAAGAGAAGAGATTAACATATGTATTGAATGATGCTGATGACAAAAAGCATTGTGCAGGCATAAATTGCTTGACTGTATTGAAGGCATCAGCGTCTGGATCTGATCACCTATTTACTGGGAGCCGCGATGGAACACTAAAAAGATGGGCTGTGGCCGAAGATGCCGCTACCTGCTCAGCAACCTTTGAGTCTCATGTGGATTGGGTTAATGATGCTGTTCTTGCAGGGGATAATACACTTGTTTCCTGTTCTTCAGATACCACCCTCAAGACGTGGGATTGCTTGTCAGATGGAACTTGTACCAGAACTCTTCGTCAGCATTCTGACTATGTCACTTGTCTTGCTGCTGCAGAAAAAAATAGCAATATTGTTGCCTCTGGTGGACTTGGAGGGGAGGTATTCATTTGGGATCTCGAAGCAGCCCTTGCTCCGGTCTCAAAGTCAGGTGATTCTGTGGAAGATGATTGTTCAAATGCCACCAATGGTTCTGGAAATGCACAACCGATTACAAGTTTACGTACTATCAGCTCTAGCAACAGCATTTCTATGCACACAACTCAGGCACATGGATATGTTCCAATCTCTGCCAAAGGCCATAAAGAATCAGTTTATGCACTGGCAATGCATGATAGTGGATCACTTCTTGTCTCTGGTGGAACAGAGAAGGTCGTGCGTGTTTGGGACCCAAGAAGTGGCTCGAAGACTATGAAACTAAGAGGGCATACAGATAATATCAGGGCTCTGCTTCTTGATTCTACTGGCAGGTTTTGCTTATCAGGATCTTCTGATTCAATGATTAGACTATGGGATCTTGGTCAGCAAAGATGTGTGCATTCTTATGCGGTGCACACAGATTCTGTTTGGGCACTTGCCAGTACGCCAACGTTTAGTCATGTATATAGTGGTGGGAGAGACCTTTCTTTGTACTTGACAGACTTGACCACAAGAGAGAGTCTTTTGCTTTGCCAAGGAGAACACCCCATTCTGCAGTTGGCTCTAGATGATGATAGCATATGGGTTGCAACAACAGATTCTTCAGTTAATAGATGGCCTGCTGAGGGGCGCAATCCTCAGAAAATTATCCAAAGAGGTGGCGCTTTCTTGGCTGGAAATTTGTCATTCTCAAGAGCAAGGGTGTCCTTAGAAGGATCTACCCTAGTCCCTGTTTATAAAGAACCAGTCTTTACAATTCCTGGAACTCCTGGAATAGTGCAACATGAAATATTAAATAATAGAAGGCATGTCTTGACAAAGGATACTGCTTCTTCGGTGAAGCTGTGGGAGATTACTAGAGGTGTTGTTATTGAGGATTACGGAAAGGTCTCGTTTGAGGAGAAAAAGGAAGAGCTTTTTGAGACTGTAAGCATTCCTGCATGGTTCACTGTGGATACCAGGCTTGGAAGTTTATCTGTGCATATGGATACCCCACAATGCTTTTCAGCGGAGATGTATTCGGCAGACCTTAATATTGTAGGAAAGCCTGAAGATGACAAGGTTAATCTAGCGCGAGAAACGCTTAAAGGGCTCTTGGCTCATTGGTTGGGGAAAAGAAAGCAAAGATCGGGAACACAAGCATCAGTGAACGGGGATGTGTCATCCGGAAAGGAAATCACTCCTAGAAGTATTACCCACTCAAGAATGGAGGTGGATAGTAATGCCGAAAATGATTCAATGGTCTATCCTCCCTTTGAATTTTCTACAGTTTCCCCTCCTTCTATCATCACTGAGGGCTCTCAGGGAGGACCATGGAGAAAGAAGATCACTGACCTAGATGGAACTGAAGATGAGAAGGACTTTCCATGGTGGTGTCTGGATTGTGTGTTGAATAATCGCCTACCTCCTAGAGAAAATACCAAGTGCAGTTTTTATCTACATCCATGTGAAGGTTCAACTGTTCAGATCCTCACACAAGGAAAACTAAGTGCACCTCGTATATTACGAATTCATAAGGTAAAGTTTTGGATTCATGGTATTTTGTGCTGCACGATGAAAAATATTGTTCTGTTGCTTTTATGA

Protein Analysis

688

Amino Acids

75.26

Weight (kDa)

6.35

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 26 - 157 7.1e-08 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 31 - 112 6.6e-06 TEP-1 second beta-propeller
WDR55 PF24796 32 - 154 1.1e-07 WDR55
WD40_Prp19 PF24814 36 - 158 6.3e-11 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 40 - 157 1.7e-17 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 40 - 157 6.5e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 42 - 155 4.8e-13 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 52 - 245 8.2e-19 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 121 - 265 1.1e-12 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 122 - 363 2.1e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 150 - 325 3.9e-27 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 180 - 340 1.3e-10 G protein beta WD-40 repeat protein
WDR55 PF24796 188 - 293 6.2e-11 WDR55
WD40_WDHD1_1st PF24817 195 - 300 2.3e-12 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 208 - 287 8.2e-10 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 209 - 303 2.5e-06 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 210 - 301 1.1e-09 EIF3I
Beta-prop_TEP1_2nd PF25047 213 - 336 8e-08 TEP-1 second beta-propeller
Beta-prop_WDR41 PF25178 213 - 330 3.8e-06 WDR41
WD40 PF00400 214 - 245 4.4e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 214 - 370 4.2e-20 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 214 - 296 4.5e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 216 - 370 4.4e-20 THOC3 beta-propeller domain
WD40_Prp19 PF24814 217 - 343 5.6e-19 Prp19 WD40 domain
WD40 PF00400 251 - 287 4.2e-07 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 254 - 367 1e-05 WDR3 second beta-propeller domain
DUF3337 PF11816 612 - 672 1.3e-08 Domain of unknown function (DUF3337)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1221
Acc36I ACCTGC 2 cut(s) 230, 804
AccB7I CCANNNNNTGG 1 cut(s) 743
AccII CGCG 2 cut(s) 177, 1558
AccIII TCCGGA 1 cut(s) 1649
AciI CCGC 4 cut(s) 175, 216, 893, 1493
AclI AACGTT 1 cut(s) 935
AclWI GGATC 7 cut(s) 157, 468, 694, 838, 867, 1207, 1945
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 3 cut(s) 1162, 1742, 1992
AcuI CTGAAG 4 cut(s) 288, 1071, 1554, 1851
AdeI CACNNNGTG 1 cut(s) 10
AfaI GTAC 4 cut(s) 352, 571, 928, 977
AfiI CCNNNNNNNGG 4 cut(s) 481, 743, 1109, 1934
AgsI TTSAA 8 cut(s) 79, 133, 522, 843, 1722, 1742, 1876, 1941
AjiI CACGTC 1 cut(s) 324
AjnI CCWGG 3 cut(s) 1243, 1252, 1442
AluBI AGCT 3 cut(s) 579, 1327, 1399
AluI AGCT 3 cut(s) 579, 1327, 1399
Alw21I GWGCWC 2 cut(s) 900, 1978
Alw26I GTCTC 6 cut(s) 246, 490, 703, 957, 1378, 1400
Alw44I GTGCAC 2 cut(s) 896, 1974
AlwI GGATC 7 cut(s) 157, 468, 694, 838, 867, 1207, 1945
AlwNI CAGNNNCTG 2 cut(s) 146, 908
Aor13HI TCCGGA 1 cut(s) 1649
AoxI GGCC 3 cut(s) 204, 643, 1100
ApaLI GTGCAC 2 cut(s) 896, 1974
ApeKI GCWGC 4 cut(s) 395, 398, 470, 2031
ApoI RAATTY 3 cut(s) 1162, 1742, 1992
AspLEI GCGC 3 cut(s) 1116, 1148, 1558
AspS9I GGNCC 2 cut(s) 733, 1793
AsuHPI GGTGA 3 cut(s) 149, 509, 1333
AvaII GGWCC 2 cut(s) 733, 1793
BaeGI GKGCMC 3 cut(s) 900, 919, 1978
BanII GRGCYC 3 cut(s) 793, 1578, 1784
BbsI GAAGAC 1 cut(s) 758
Bbv12I GWGCWC 2 cut(s) 900, 1978
BbvCI CCTCAGC 1 cut(s) 1107
BbvI GCAGC 4 cut(s) 382, 385, 482, 2018
BccI CCATC 5 cut(s) 173, 189, 335, 1092, 1817
BciT130I CCWGG 3 cut(s) 1245, 1254, 1444
BciVI GTATCC 3 cut(s) 1300, 1432, 1465
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 6 cut(s) 246, 490, 703, 957, 1378, 1400
BfaI CTAG 9 cut(s) 39, 582, 1049, 1208, 1341, 1553, 1668, 1820, 1893
BfmI CTRYAG 3 cut(s) 399, 1037, 1749
BfoI RGCGCY 1 cut(s) 1149
BfuAI ACCTGC 2 cut(s) 230, 804
BfuI GTATCC 3 cut(s) 1300, 1432, 1465
BisI GCNGC 6 cut(s) 175, 216, 396, 399, 471, 2032
BlpI GCTNAGC 1 cut(s) 226
BlsI GCNGC 6 cut(s) 176, 217, 397, 400, 472, 2033
Bme1390I CCNGG 3 cut(s) 1245, 1254, 1444
Bme18I GGWCC 2 cut(s) 733, 1793
BmgBI CACGTC 1 cut(s) 324
BmgT120I GGNCC 2 cut(s) 733, 1793
BmiI GGNNCC 3 cut(s) 173, 734, 735
BmrFI CCNGG 3 cut(s) 1245, 1254, 1444
BmrI ACTGGG 1 cut(s) 177
BmsI GCATC 5 cut(s) 73, 146, 202, 253, 1634
BmuI ACTGGG 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 758
Bpu10I CCTNAGC 1 cut(s) 1107
Bpu1102I GCTNAGC 1 cut(s) 226
BpuEI CTTGAG 3 cut(s) 302, 1159, 1669
BsaAI YACGTR 1 cut(s) 569
BsaI GGTCTC 3 cut(s) 490, 957, 1378
BsaJI CCNNGG 3 cut(s) 1018, 1796, 1850
BsaWI WCCGGW 2 cut(s) 481, 1649
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 4 cut(s) 481, 743, 1109, 1934
Bse1I ACTGG 5 cut(s) 172, 672, 814, 924, 1229
Bse3DI GCAATG 1 cut(s) 678
BseAI TCCGGA 1 cut(s) 1649
BseBI CCWGG 3 cut(s) 1245, 1254, 1444
BseDI CCNNGG 3 cut(s) 1018, 1796, 1850
BseGI GGATG 4 cut(s) 29, 1645, 1646, 1924
BseLI CCNNNNNNNGG 4 cut(s) 481, 743, 1109, 1934
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 6 cut(s) 240, 623, 1098, 1136, 1767, 1799
BseNI ACTGG 5 cut(s) 172, 672, 814, 924, 1229
BseRI GAGGAG 1 cut(s) 1397
BseSI GKGCMC 3 cut(s) 900, 919, 1978
BseXI GCAGC 4 cut(s) 382, 385, 482, 2018
BseYI CCCAGC 1 cut(s) 20
BsgI GTGCAG 3 cut(s) 126, 1930, 2017
Bsh1236I CGCG 2 cut(s) 177, 1558
BshFI GGCC 3 cut(s) 206, 645, 1102
BsiHKAI GWGCWC 2 cut(s) 900, 1978
BsiSI CCGG 2 cut(s) 482, 1650
BslFI GGGAC 2 cut(s) 746, 1196
BslI CCNNNNNNNGG 4 cut(s) 481, 743, 1109, 1934
BsmAI GTCTC 6 cut(s) 246, 490, 703, 957, 1378, 1400
BsmFI GGGAC 2 cut(s) 746, 1196
BsmI GAATGC 3 cut(s) 370, 883, 1416
BsnI GGCC 3 cut(s) 206, 645, 1102
Bso31I GGTCTC 3 cut(s) 490, 957, 1378
Bsp1286I GDGCHC 6 cut(s) 793, 900, 919, 1578, 1784, 1978
Bsp13I TCCGGA 1 cut(s) 1649
Bsp1720I GCTNAGC 1 cut(s) 226
Bsp19I CCATGG 2 cut(s) 1796, 1850
BspACI CCGC 4 cut(s) 175, 216, 893, 1493
BspANI GGCC 3 cut(s) 206, 645, 1102
BspCNI CTCAG 6 cut(s) 239, 622, 1099, 1135, 1768, 1798
BspEI TCCGGA 1 cut(s) 1649
BspFNI CGCG 2 cut(s) 177, 1558
BspLI GGNNCC 3 cut(s) 173, 734, 735
BspMAI CTGCAG 2 cut(s) 403, 1041
BspMI ACCTGC 2 cut(s) 230, 804
BspPI GGATC 7 cut(s) 157, 468, 694, 838, 867, 1207, 1945
BspQI GCTCTTC 1 cut(s) 1389
BspTNI GGTCTC 3 cut(s) 490, 957, 1378
BsrDI GCAATG 1 cut(s) 678
BsrI ACTGG 5 cut(s) 172, 672, 814, 924, 1229
BssECI CCNNGG 3 cut(s) 1018, 1796, 1850
BssT1I CCWWGG 3 cut(s) 1018, 1796, 1850
Bst2UI CCWGG 3 cut(s) 1245, 1254, 1444
Bst4CI ACNGT 5 cut(s) 127, 1411, 1435, 1753, 1945
Bst6I CTCTTC 3 cut(s) 53, 366, 1389
BstAPI GCANNNNNTGC 3 cut(s) 117, 476, 819
BstBAI YACGTR 1 cut(s) 569
BstC8I GCNNGC 2 cut(s) 109, 1104
BstDEI CTNAG 9 cut(s) 226, 609, 764, 1107, 1122, 1192, 1776, 1785, 1970
BstDSI CCRYGG 2 cut(s) 1796, 1850
BstF5I GGATG 4 cut(s) 29, 1645, 1646, 1924
BstFNI CGCG 2 cut(s) 177, 1558
BstH2I RGCGCY 1 cut(s) 1149
BstHHI GCGC 3 cut(s) 1116, 1148, 1558
BstMAI GTCTC 6 cut(s) 246, 490, 703, 957, 1378, 1400
BstMWI GCNNNNNNNGC 8 cut(s) 117, 212, 272, 476, 819, 1154, 1564, 1573
BstNI CCWGG 3 cut(s) 1245, 1254, 1444
BstNSI RCATGY 1 cut(s) 1295
BstSCI CCNGG 3 cut(s) 1243, 1252, 1442
BstSFI CTRYAG 3 cut(s) 399, 1037, 1749
BstSLI GKGCMC 3 cut(s) 900, 919, 1978
BstSNI TACGTA 1 cut(s) 569
BstUI CGCG 2 cut(s) 177, 1558
BstV1I GCAGC 4 cut(s) 382, 385, 482, 2018
BstV2I GAAGAC 1 cut(s) 758
BstX2I RGATCY 6 cut(s) 149, 460, 830, 859, 1199, 1950
BstXI CCANNNNNNTGG 1 cut(s) 1142
BstYI RGATCY 6 cut(s) 149, 460, 830, 859, 1199, 1950
BsuI GTATCC 3 cut(s) 1300, 1432, 1465
BsuRI GGCC 3 cut(s) 206, 645, 1102
BtgI CCRYGG 2 cut(s) 1796, 1850
BtgZI GCGATG 1 cut(s) 192
BtrI CACGTC 1 cut(s) 324
BtsCI GGATG 4 cut(s) 29, 1645, 1646, 1924
BtsIMutI CAGTG 5 cut(s) 665, 1431, 1635, 1773, 1812
BveI ACCTGC 2 cut(s) 230, 804
Cac8I GCNNGC 2 cut(s) 109, 1104
CaiI CAGNNNCTG 2 cut(s) 146, 908
CfoI GCGC 3 cut(s) 1116, 1148, 1558
Cfr13I GGNCC 2 cut(s) 733, 1793
CseI GACGC 1 cut(s) 132
Csp6I GTAC 4 cut(s) 351, 570, 927, 976
CviQI GTAC 4 cut(s) 351, 570, 927, 976
DdeI CTNAG 9 cut(s) 226, 609, 764, 1107, 1122, 1192, 1776, 1785, 1970
DraIII CACNNNGTG 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 3 cut(s) 53, 366, 1389
EarI CTCTTC 3 cut(s) 53, 366, 1389
Eco105I TACGTA 1 cut(s) 569
Eco130I CCWWGG 3 cut(s) 1018, 1796, 1850
Eco24I GRGCYC 3 cut(s) 793, 1578, 1784
Eco31I GGTCTC 3 cut(s) 490, 957, 1378
Eco47I GGWCC 2 cut(s) 733, 1793
Eco57I CTGAAG 4 cut(s) 288, 1071, 1554, 1851
EcoO109I RGGNCCY 1 cut(s) 733
EcoRI GAATTC 1 cut(s) 1992
EcoRII CCWGG 3 cut(s) 1243, 1252, 1442
EcoT14I CCWWGG 3 cut(s) 1018, 1796, 1850
EcoT22I ATGCAT 1 cut(s) 678
EcoT38I GRGCYC 3 cut(s) 793, 1578, 1784
ErhI CCWWGG 3 cut(s) 1018, 1796, 1850
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FaqI GGGAC 2 cut(s) 746, 1196
FauNDI CATATG 3 cut(s) 70, 1064, 1467
FbaI TGATCA 1 cut(s) 154
Fnu4HI GCNGC 6 cut(s) 175, 216, 396, 399, 471, 2032
FokI GGATG 4 cut(s) 16, 1633, 1652, 1911
FriOI GRGCYC 3 cut(s) 793, 1578, 1784
Fsp4HI GCNGC 6 cut(s) 175, 216, 396, 399, 471, 2032
FspBI CTAG 9 cut(s) 39, 582, 1049, 1208, 1341, 1553, 1668, 1820, 1893
GlaI GCGC 3 cut(s) 1115, 1147, 1557
GluI GCNGC 6 cut(s) 175, 216, 396, 399, 471, 2032
GsaI CCCAGC 1 cut(s) 24
HaeII RGCGCY 1 cut(s) 1149
HaeIII GGCC 3 cut(s) 206, 645, 1102
HapII CCGG 2 cut(s) 482, 1650
HgaI GACGC 1 cut(s) 132
HhaI GCGC 3 cut(s) 1116, 1148, 1558
Hin6I GCGC 3 cut(s) 1114, 1146, 1556
HinP1I GCGC 3 cut(s) 1114, 1146, 1556
HpaII CCGG 2 cut(s) 482, 1650
HphI GGTGA 3 cut(s) 149, 509, 1333
Hpy166II GTNNAC 6 cut(s) 434, 566, 898, 1431, 1633, 1976
Hpy188I TCNGA 7 cut(s) 154, 307, 340, 376, 838, 1125, 1950
Hpy8I GTNNAC 6 cut(s) 434, 566, 898, 1431, 1633, 1976
HpyAV CCTTC 7 cut(s) 127, 709, 1190, 1281, 1773, 1834, 1928
HpyCH4III ACNGT 5 cut(s) 127, 1411, 1435, 1753, 1945
HpyCH4IV ACGT 3 cut(s) 323, 568, 935
HpyF10VI GCNNNNNNNGC 8 cut(s) 117, 212, 272, 476, 819, 1154, 1564, 1573
HpyF3I CTNAG 9 cut(s) 226, 609, 764, 1107, 1122, 1192, 1776, 1785, 1970
HpySE526I ACGT 3 cut(s) 323, 568, 935
HspAI GCGC 3 cut(s) 1114, 1146, 1556
KflI GGGWCCC 1 cut(s) 733
Kpn2I TCCGGA 1 cut(s) 1649
Ksp22I TGATCA 1 cut(s) 154
LguI GCTCTTC 1 cut(s) 1389
LmnI GCTCC 2 cut(s) 171, 484
Lsp1109I GCAGC 4 cut(s) 382, 385, 482, 2018
LweI GCATC 5 cut(s) 73, 146, 202, 253, 1634
MaeI CTAG 9 cut(s) 39, 582, 1049, 1208, 1341, 1553, 1668, 1820, 1893
MaeII ACGT 3 cut(s) 323, 568, 935
MaeIII GTNAC 1 cut(s) 382
MflI RGATCY 6 cut(s) 149, 460, 830, 859, 1199, 1950
MhlI GDGCHC 6 cut(s) 793, 900, 919, 1578, 1784, 1978
MluCI AATT 7 cut(s) 34, 115, 1128, 1162, 1239, 1742, 1992
MlyI GAGTC 2 cut(s) 248, 1012
Mph1103I ATGCAT 1 cut(s) 678
MroI TCCGGA 1 cut(s) 1649
MseI TTAA 7 cut(s) 65, 258, 1092, 1277, 1515, 1548, 1569
MslI CAYNNNNRTG 6 cut(s) 596, 621, 681, 888, 1855, 1929
MspA1I CMGCKG 1 cut(s) 1493
MspI CCGG 2 cut(s) 482, 1650
MspR9I CCNGG 3 cut(s) 1245, 1254, 1444
Mva1269I GAATGC 3 cut(s) 370, 883, 1416
MvaI CCWGG 3 cut(s) 1245, 1254, 1444
MvnI CGCG 2 cut(s) 177, 1558
MwoI GCNNNNNNNGC 8 cut(s) 117, 212, 272, 476, 819, 1154, 1564, 1573
NcoI CCATGG 2 cut(s) 1796, 1850
NdeI CATATG 3 cut(s) 70, 1064, 1467
NlaIV GGNNCC 3 cut(s) 173, 734, 735
NmuCI GTSAC 1 cut(s) 382
NsiI ATGCAT 1 cut(s) 678
NspI RCATGY 1 cut(s) 1295
PciSI GCTCTTC 1 cut(s) 1389
PcsI WCGNNNNNNNCGW 1 cut(s) 1987
PctI GAATGC 3 cut(s) 370, 883, 1416
PfeI GAWTC 8 cut(s) 500, 653, 803, 839, 905, 1082, 1718, 2014
PflFI GACNNNGTC 1 cut(s) 380
PflMI CCANNNNNTGG 1 cut(s) 743
PfoI TCCNGGA 2 cut(s) 1243, 1252
PkrI GCNGC 6 cut(s) 176, 217, 397, 400, 472, 2033
PleI GAGTC 2 cut(s) 247, 1011
PpsI GAGTC 2 cut(s) 247, 1011
Ppu21I YACGTR 1 cut(s) 569
PpuMI RGGWCCY 1 cut(s) 733
PsiI TTATAA 1 cut(s) 1221
Psp1406I AACGTT 1 cut(s) 935
Psp5II RGGWCCY 1 cut(s) 733
Psp6I CCWGG 3 cut(s) 1243, 1252, 1442
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 3 cut(s) 1243, 1252, 1442
PspN4I GGNNCC 3 cut(s) 173, 734, 735
PspPI GGNCC 2 cut(s) 733, 1793
PspPPI RGGWCCY 1 cut(s) 733
PstI CTGCAG 2 cut(s) 403, 1041
PstNI CAGNNNCTG 2 cut(s) 146, 908
PsuI RGATCY 6 cut(s) 149, 460, 830, 859, 1199, 1950
PsyI GACNNNGTC 1 cut(s) 380
RsaI GTAC 4 cut(s) 352, 571, 928, 977
RsaNI GTAC 4 cut(s) 351, 570, 927, 976
RseI CAYNNNNRTG 6 cut(s) 596, 621, 681, 888, 1855, 1929
SapI GCTCTTC 1 cut(s) 1389
SaqAI TTAA 7 cut(s) 65, 258, 1092, 1277, 1515, 1548, 1569
SatI GCNGC 6 cut(s) 175, 216, 396, 399, 471, 2032
Sau96I GGNCC 2 cut(s) 733, 1793
SchI GAGTC 2 cut(s) 248, 1012
ScrFI CCNGG 3 cut(s) 1245, 1254, 1444
SduI GDGCHC 6 cut(s) 793, 900, 919, 1578, 1784, 1978
SfaNI GCATC 5 cut(s) 73, 146, 202, 253, 1634
SfcI CTRYAG 3 cut(s) 399, 1037, 1749
SinI GGWCC 2 cut(s) 733, 1793
SmiMI CAYNNNNRTG 6 cut(s) 596, 621, 681, 888, 1855, 1929
SmlI CTYRAG 3 cut(s) 317, 1174, 1684
SmoI CTYRAG 3 cut(s) 317, 1174, 1684
SnaBI TACGTA 1 cut(s) 569
Sse9I AATT 7 cut(s) 34, 115, 1128, 1162, 1239, 1742, 1992
SsiI CCGC 4 cut(s) 175, 216, 893, 1493
SspI AATATT 4 cut(s) 418, 1275, 1519, 2047
SspMI CTAG 9 cut(s) 39, 582, 1049, 1208, 1341, 1553, 1668, 1820, 1893
StyD4I CCNGG 3 cut(s) 1243, 1252, 1442
StyI CCWWGG 3 cut(s) 1018, 1796, 1850
TaaI ACNGT 5 cut(s) 127, 1411, 1435, 1753, 1945
TaiI ACGT 3 cut(s) 326, 571, 938
TaqI TCGA 2 cut(s) 465, 749
TasI AATT 7 cut(s) 34, 115, 1128, 1162, 1239, 1742, 1992
TatI WGTACW 1 cut(s) 975
TauI GCSGC 2 cut(s) 177, 218
TfiI GAWTC 8 cut(s) 500, 653, 803, 839, 905, 1082, 1718, 2014
Tru1I TTAA 7 cut(s) 65, 258, 1092, 1277, 1515, 1548, 1569
Tru9I TTAA 7 cut(s) 65, 258, 1092, 1277, 1515, 1548, 1569
TscAI CASTG 5 cut(s) 672, 1438, 1635, 1780, 1819
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 4 cut(s) 395, 398, 470, 2031
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 6 cut(s) 442, 773, 1284, 1985, 2006, 2054
TspGWI ACGGA 1 cut(s) 1380
TspRI CASTG 5 cut(s) 672, 1438, 1635, 1780, 1819
Tth111I GACNNNGTC 1 cut(s) 380
Van91I CCANNNNNTGG 1 cut(s) 743
VneI GTGCAC 2 cut(s) 896, 1974
VpaK11BI GGWCC 2 cut(s) 733, 1793
XapI RAATTY 3 cut(s) 1162, 1742, 1992
XbaI TCTAGA 1 cut(s) 1048
XceI RCATGY 1 cut(s) 1295
XcmI CCANNNNNNNNNTGG 1 cut(s) 1039
XspI CTAG 9 cut(s) 39, 582, 1049, 1208, 1341, 1553, 1668, 1820, 1893
Zsp2I ATGCAT 1 cut(s) 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.