Rroxscaffold_6G00429610

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007420
Physical Location & Seq
Forward (+)
49838282 .. 49847127
8846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00429610.1

Sequence Viewer

Length: 2421 bp
ATGCACCGAGTGGGTAGTGCTGGGAACACATCCAATTCTAGTCGCCCTCGTAAAGAGAAGAGATTAACATATGTATTGAATGATGCTGATGACAAAAAGCATTGTGCAGGCATAAATTGTTTGACTGTATTGAAGGCATCAGCGTCTGGATCTGATCACCTATTTACTGGGAGCCGCGATGGAACATTAAAAAGATGGGCTGTGGCCGAAGATGCTGCTACCTGCTCAGCAACCTTTGAGTCTCATGTGGATTGGGTTAATGATGCTGTTCTTGCAGGGGATAATACACTTGTTTCCTGTTCTTCAGATACCACCCTCAAGACATGGGATTGCTTGTCAGATGGAACTTGTACCAGAACTCTTCGTCAGCATTCTGACTATGTCACTTGTCTTGCTGCCGCAGAAAAAAATAGCAATATTGTTGCCTCTGGTGGACTTGGAGGGGAGGTATTCATTTGGGATCTCGAAGCAGCCCTTGCTCCGGTCTCAAAGTCAGGTGATTCTGTGGAAGATGATTGTTCAAATGCCACCAATGGTTCTGGAAATGCACAACCGATTACAAGTTTACGTACTATCAGCTCTAGCAACAGCATTTCTATGCACACAACTCAGGCACATGGATATGTTCCAATCTCTGCCAAAGGCCATAAAGAATCAGTTTATGCACTGGCAATGCATGATAGTGGATCACTTCTTGTCTCTGGTGGAACAGAGAAGGTCGTGCGTGTTTGGGACCCAAGAAGTGGCTCGAAGACTATGAAACTAAGAGGGCATACAGATAATATCAGGGCTCTGCTTCTTGATTCTACTGGCAGGTTTTGCTTGTCAGGATCTTCTGATTCAATGATTAGACTATGGGATCTTGGTCAGCAAAGATGCGTGCATTCTTATGCGGTGCACACAGATTCTGTTTGGGCACTTGCCAGTACGCCAACGTTTAGTCATGTATATAGTGGTGGGAGGGACCTTTCTTTGTACTTGACAGACTTGACCACAAGAGAGAGTCTTTTGCTTTGCCAAGGAGAACACCCCATTCTACAGTTGGCTCTAGATGATGATAGCATATGGGTTGCAACAACAGATTCTTCAGTTAATAGATGGCCTGCTGAGGGGCGCAATCCTCAGAAAATTATCCAAAGAGGTGGCGCTTTCTTGGCCGGAAATTTGTCATTCTCAAGAGCAAGGGTGTCCTTAGAAGGATCTACCCTAGTCCCTGTTTATAAAGAACCAGTTTTTACAATTCCTGGAACTCCTGGAATAGTGCAACATGAAATATTAAATAATAGAAGGCATGTCTTGACAAAGGTCTCATTTGAGGAGAAAAAGGAAGAGCTTTTTGAGACTGTAAGCATTCCTGCATGGTTCACTGTGGATACCAGGCTTGGAAGTTTATCTGTACATATGGATACGCCGCAATGCTTTTCAGCAGAGATGTATTCGGCAGACCTTAATATTGTAGGAAAGCCTGAAGATGACAAGCGCGAGAAACACTTAAAGGGCTCTTGGCTCATTGGTTGGGGAAAAGAAAGAAAGCAAAGATCGGGAACACAAGCATCAGTGAACGGGGATGTGTCATCCGGAAAGGAAATCACTCCTAGAAGTATTACCCACTCAAGAATGGAGGTGGATAGTAATGCCGAAAATGATTCAATGGTCTATCCTCCCTTTGAATTTTCTACTGTTTCCCCTCCTTCTATCATCACTGAGGGCTCTCAGGGAGGAACATGGAGAAAGAAGATCACTGACCTAGATGGAACTGAAGATGAGAAGGACTTTCCATGGTGCAGTTTTTATCTACATCCATGTGAAGGTTCAACTGTTCAGATCCTCACACAAGGAAAACTAAGTGCTCCTCGTATATTACGAATTCATAAGGTTGTTAACTATGTAGTTGAAAAGATGGTCCTGGACAAACCATTAGATAGTGCAAATCCTGATGCAACATTTGCTCCTGGTCTTGCTGGAGGGCCAGGACAACTCTCTGCCATTGGAGATGGATCTTTTCGGTCTGGATTGAAGCCATGGCAGAAGCTTAAACCTTCTATAGAGATATTGTGCAACAATCAGGCAAGTCTTGTCGCCAGAAATGAGCTTAGCCACAGTGCGGGCTTATGTATGGAAGAAACCCGAGGACCTGGTTCTCAATTACAGGGTGGTTCAGGGCAGGTGATTCTGCACAATTTTGTGGAGGTGAGATCGTCCCTCTCGTATGATTTCTCCTTCACCCCCATTTTAACGTATTCGGCGCTTTTGTTAGATTGGTATACCTTGTACGTTTTTGAGCGGGAGAATTCCTACTGCAAGACTGAAGCATATGCTGCATATTCCAATGGGGTCCCCAATAGTTACCCACTCATACTTTTGATTGGTTCTCTGTACTTCTTTAGTGCCGTATGGGACTCTGGAGTTGAGCCCAACTGA

Protein Analysis

806

Amino Acids

87.81

Weight (kDa)

5.88

Isoelectric Point (pI)

38.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 26 - 157 9.5e-08 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 31 - 112 8.1e-06 TEP-1 second beta-propeller
WDR55 PF24796 32 - 154 1.3e-07 WDR55
WD40_Prp19 PF24814 36 - 158 9e-11 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 40 - 157 2.1e-17 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 41 - 157 8.3e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 42 - 155 6.1e-13 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 52 - 245 9.2e-19 THOC3 beta-propeller domain
WD40_Gbeta PF25391 119 - 340 4.9e-12 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 123 - 363 2.8e-08 Echinoderm microtubule-associated protein first beta-propeller
WDR55 PF24796 188 - 292 9.9e-11 WDR55
WD40_CDC20-Fz PF24807 188 - 265 1.9e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 194 - 325 1.5e-26 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 196 - 300 3.1e-12 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 210 - 287 9.9e-10 WDR3 second beta-propeller domain
EIF3I PF24805 210 - 301 1.4e-09 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 211 - 303 3.4e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 213 - 335 1.2e-07 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 213 - 296 5.6e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR41 PF25178 213 - 330 4.9e-06 WDR41
WD40 PF00400 214 - 245 5.4e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 214 - 370 6e-20 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 216 - 370 5.7e-20 THOC3 beta-propeller domain
WD40_Prp19 PF24814 217 - 342 7.3e-19 Prp19 WD40 domain
WD40 PF00400 251 - 287 5.1e-07 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 254 - 367 9.8e-06 WDR3 second beta-propeller domain
DUF3337 PF11816 590 - 690 7.8e-06 Domain of unknown function (DUF3337)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1221
AarI CACCTGC 1 cut(s) 2155
Acc36I ACCTGC 3 cut(s) 230, 804, 2155
AccB7I CCANNNNNTGG 1 cut(s) 743
AccBSI CCGCTC 1 cut(s) 2284
AccI GTMKAC 1 cut(s) 2264
AccII CGCG 2 cut(s) 177, 1482
AccIII TCCGGA 1 cut(s) 1577
AciI CCGC 6 cut(s) 175, 399, 893, 1412, 2105, 2284
AclI AACGTT 1 cut(s) 935
AclWI GGATC 8 cut(s) 157, 468, 694, 838, 867, 1207, 1819, 2005
AcoI YGGCCR 2 cut(s) 204, 1155
AcsI RAATTY 4 cut(s) 1162, 1670, 1866, 2290
AcuI CTGAAG 5 cut(s) 288, 1071, 1488, 1779, 2328
AdeI CACNNNGTG 1 cut(s) 10
AfaI GTAC 7 cut(s) 352, 571, 928, 977, 1398, 2273, 2378
AfiI CCNNNNNNNGG 5 cut(s) 481, 743, 1109, 1808, 2104
AgsI TTSAA 9 cut(s) 79, 133, 522, 843, 1650, 1670, 1815, 1895, 2017
AjnI CCWGG 7 cut(s) 1243, 1252, 1376, 1905, 1951, 1969, 2134
AluBI AGCT 4 cut(s) 579, 1333, 2032, 2092
AluI AGCT 4 cut(s) 579, 1333, 2032, 2092
Alw21I GWGCWC 2 cut(s) 900, 1852
Alw26I GTCTC 5 cut(s) 246, 490, 703, 1312, 1334
Alw44I GTGCAC 1 cut(s) 896
AlwI GGATC 8 cut(s) 157, 468, 694, 838, 867, 1207, 1819, 2005
AlwNI CAGNNNCTG 2 cut(s) 146, 908
Ama87I CYCGRG 1 cut(s) 2127
Aor13HI TCCGGA 1 cut(s) 1577
AoxI GGCC 5 cut(s) 204, 643, 1100, 1155, 1967
ApaLI GTGCAC 1 cut(s) 896
ApeKI GCWGC 4 cut(s) 215, 395, 470, 2318
ApoI RAATTY 4 cut(s) 1162, 1670, 1866, 2290
AspLEI GCGC 4 cut(s) 1116, 1148, 1482, 2248
AspS9I GGNCC 6 cut(s) 733, 964, 1903, 1967, 2132, 2335
AsuHPI GGTGA 5 cut(s) 149, 509, 2179, 2203, 2215
AvaI CYCGRG 1 cut(s) 2127
AvaII GGWCC 5 cut(s) 733, 964, 1903, 2132, 2335
BaeGI GKGCMC 2 cut(s) 900, 919
BanII GRGCYC 4 cut(s) 793, 1502, 1712, 2415
BbsI GAAGAC 1 cut(s) 758
Bbv12I GWGCWC 2 cut(s) 900, 1852
BbvCI CCTCAGC 1 cut(s) 1107
BbvI GCAGC 4 cut(s) 202, 382, 482, 2305
BccI CCATC 7 cut(s) 173, 189, 335, 1092, 1745, 1894, 1988
BceAI ACGGC 1 cut(s) 2375
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 7 cut(s) 1245, 1254, 1378, 1907, 1953, 1971, 2136
BciVI GTATCC 2 cut(s) 1366, 1399
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 5 cut(s) 246, 490, 703, 1312, 1334
BfaI CTAG 6 cut(s) 39, 582, 1049, 1208, 1596, 1748
BfmI CTRYAG 2 cut(s) 1037, 2043
BfoI RGCGCY 2 cut(s) 1149, 2249
BfuAI ACCTGC 3 cut(s) 230, 804, 2155
BfuI GTATCC 2 cut(s) 1366, 1399
BisI GCNGC 7 cut(s) 175, 216, 396, 399, 471, 1412, 2319
BlpI GCTNAGC 2 cut(s) 226, 2093
BlsI GCNGC 7 cut(s) 176, 217, 397, 400, 472, 1413, 2320
Bme1390I CCNGG 7 cut(s) 1245, 1254, 1378, 1907, 1953, 1971, 2136
Bme18I GGWCC 5 cut(s) 733, 964, 1903, 2132, 2335
BmeT110I CYCGRG 1 cut(s) 2127
BmgT120I GGNCC 6 cut(s) 733, 964, 1903, 1967, 2132, 2335
BmiI GGNNCC 6 cut(s) 173, 734, 735, 965, 2336, 2337
BmrFI CCNGG 7 cut(s) 1245, 1254, 1378, 1907, 1953, 1971, 2136
BmrI ACTGGG 1 cut(s) 177
BmsI GCATC 7 cut(s) 73, 146, 202, 253, 866, 1562, 1927
BmuI ACTGGG 1 cut(s) 177
BoxI GACNNNNGTC 1 cut(s) 1304
BpiI GAAGAC 1 cut(s) 758
BpmI CTGGAG 1 cut(s) 1983
Bpu10I CCTNAGC 1 cut(s) 1107
Bpu1102I GCTNAGC 2 cut(s) 226, 2093
BpuEI CTTGAG 3 cut(s) 302, 1159, 1597
BsaAI YACGTR 1 cut(s) 569
BsaI GGTCTC 2 cut(s) 490, 1312
BsaJI CCNNGG 4 cut(s) 1018, 1778, 2021, 2128
BsaWI WCCGGW 2 cut(s) 481, 1577
BsaXI ACNNNNNCTCC 4 cut(s) 432, 462, 1933, 1963
Bsc4I CCNNNNNNNGG 5 cut(s) 481, 743, 1109, 1808, 2104
Bse1I ACTGG 5 cut(s) 172, 672, 814, 924, 1229
Bse3DI GCAATG 2 cut(s) 678, 1421
BseAI TCCGGA 1 cut(s) 1577
BseBI CCWGG 7 cut(s) 1245, 1254, 1378, 1907, 1953, 1971, 2136
BseDI CCNNGG 4 cut(s) 1018, 1778, 2021, 2128
BseGI GGATG 4 cut(s) 29, 1573, 1574, 1798
BseLI CCNNNNNNNGG 5 cut(s) 481, 743, 1109, 1808, 2104
BseMI GCAATG 2 cut(s) 678, 1421
BseMII CTCAG 6 cut(s) 240, 623, 1098, 1136, 1695, 1727
BseNI ACTGG 5 cut(s) 172, 672, 814, 924, 1229
BseRI GAGGAG 2 cut(s) 1331, 1842
BseSI GKGCMC 2 cut(s) 900, 919
BseXI GCAGC 4 cut(s) 202, 382, 482, 2305
BseYI CCCAGC 1 cut(s) 20
BsgI GTGCAG 3 cut(s) 126, 1804, 2159
Bsh1236I CGCG 2 cut(s) 177, 1482
BshFI GGCC 5 cut(s) 206, 645, 1102, 1157, 1969
BsiHKAI GWGCWC 2 cut(s) 900, 1852
BsiHKCI CYCGRG 1 cut(s) 2127
BsiSI CCGG 3 cut(s) 482, 1158, 1578
BslFI GGGAC 6 cut(s) 746, 977, 1196, 2185, 2321, 2411
BslI CCNNNNNNNGG 5 cut(s) 481, 743, 1109, 1808, 2104
BsmAI GTCTC 5 cut(s) 246, 490, 703, 1312, 1334
BsmFI GGGAC 6 cut(s) 746, 977, 1196, 2185, 2321, 2411
BsmI GAATGC 3 cut(s) 370, 883, 1350
BsnI GGCC 5 cut(s) 206, 645, 1102, 1157, 1969
Bso31I GGTCTC 2 cut(s) 490, 1312
BsoBI CYCGRG 1 cut(s) 2127
Bsp1286I GDGCHC 7 cut(s) 793, 900, 919, 1502, 1712, 1852, 2415
Bsp13I TCCGGA 1 cut(s) 1577
Bsp1407I TGTACA 1 cut(s) 1396
Bsp1720I GCTNAGC 2 cut(s) 226, 2093
Bsp19I CCATGG 2 cut(s) 1778, 2021
BspACI CCGC 6 cut(s) 175, 399, 893, 1412, 2105, 2284
BspANI GGCC 5 cut(s) 206, 645, 1102, 1157, 1969
BspCNI CTCAG 6 cut(s) 239, 622, 1099, 1135, 1696, 1726
BspEI TCCGGA 1 cut(s) 1577
BspFNI CGCG 2 cut(s) 177, 1482
BspLI GGNNCC 6 cut(s) 173, 734, 735, 965, 2336, 2337
BspMI ACCTGC 3 cut(s) 230, 804, 2155
BspPI GGATC 8 cut(s) 157, 468, 694, 838, 867, 1207, 1819, 2005
BspQI GCTCTTC 1 cut(s) 1323
BspTNI GGTCTC 2 cut(s) 490, 1312
BsrBI CCGCTC 1 cut(s) 2284
BsrDI GCAATG 2 cut(s) 678, 1421
BsrGI TGTACA 1 cut(s) 1396
BsrI ACTGG 5 cut(s) 172, 672, 814, 924, 1229
BssECI CCNNGG 4 cut(s) 1018, 1778, 2021, 2128
BssNAI GTATAC 1 cut(s) 2265
BssT1I CCWWGG 3 cut(s) 1018, 1778, 2021
Bst1107I GTATAC 1 cut(s) 2265
Bst2UI CCWGG 7 cut(s) 1245, 1254, 1378, 1907, 1953, 1971, 2136
Bst4CI ACNGT 7 cut(s) 127, 1041, 1345, 1369, 1681, 1819, 2102
Bst6I CTCTTC 3 cut(s) 53, 366, 1323
BstAPI GCANNNNNTGC 4 cut(s) 476, 819, 1946, 2318
BstAUI TGTACA 1 cut(s) 1396
BstBAI YACGTR 1 cut(s) 569
BstC8I GCNNGC 4 cut(s) 109, 881, 1104, 2107
BstDSI CCRYGG 2 cut(s) 1778, 2021
BstF5I GGATG 4 cut(s) 29, 1573, 1574, 1798
BstFNI CGCG 2 cut(s) 177, 1482
BstH2I RGCGCY 2 cut(s) 1149, 2249
BstHHI GCGC 4 cut(s) 1116, 1148, 1482, 2248
BstMAI GTCTC 5 cut(s) 246, 490, 703, 1312, 1334
BstMWI GCNNNNNNNGC 7 cut(s) 212, 272, 476, 819, 1154, 1946, 2318
BstNI CCWGG 7 cut(s) 1245, 1254, 1378, 1907, 1953, 1971, 2136
BstNSI RCATGY 1 cut(s) 1295
BstPAI GACNNNNGTC 1 cut(s) 1304
BstSCI CCNGG 7 cut(s) 1243, 1252, 1376, 1905, 1951, 1969, 2134
BstSFI CTRYAG 2 cut(s) 1037, 2043
BstSLI GKGCMC 2 cut(s) 900, 919
BstSNI TACGTA 1 cut(s) 569
BstUI CGCG 2 cut(s) 177, 1482
BstV1I GCAGC 4 cut(s) 202, 382, 482, 2305
BstV2I GAAGAC 1 cut(s) 758
BstX2I RGATCY 7 cut(s) 149, 460, 830, 859, 1199, 1824, 1997
BstXI CCANNNNNNTGG 1 cut(s) 1142
BstYI RGATCY 7 cut(s) 149, 460, 830, 859, 1199, 1824, 1997
BstZ17I GTATAC 1 cut(s) 2265
BsuI GTATCC 2 cut(s) 1366, 1399
BsuRI GGCC 5 cut(s) 206, 645, 1102, 1157, 1969
BtgI CCRYGG 2 cut(s) 1778, 2021
BtgZI GCGATG 1 cut(s) 192
BtsCI GGATG 4 cut(s) 29, 1573, 1574, 1798
BtsIMutI CAGTG 6 cut(s) 665, 1365, 1563, 1701, 1740, 2107
BveI ACCTGC 3 cut(s) 230, 804, 2155
Cac8I GCNNGC 4 cut(s) 109, 881, 1104, 2107
CaiI CAGNNNCTG 2 cut(s) 146, 908
CfoI GCGC 4 cut(s) 1116, 1148, 1482, 2248
Cfr13I GGNCC 6 cut(s) 733, 964, 1903, 1967, 2132, 2335
CseI GACGC 1 cut(s) 132
CsiI ACCWGGT 1 cut(s) 2134
Csp6I GTAC 7 cut(s) 351, 570, 927, 976, 1397, 2272, 2377
CviQI GTAC 7 cut(s) 351, 570, 927, 976, 1397, 2272, 2377
DraIII CACNNNGTG 1 cut(s) 10
EaeI YGGCCR 2 cut(s) 204, 1155
Eam1104I CTCTTC 3 cut(s) 53, 366, 1323
EarI CTCTTC 3 cut(s) 53, 366, 1323
Eco105I TACGTA 1 cut(s) 569
Eco130I CCWWGG 3 cut(s) 1018, 1778, 2021
Eco24I GRGCYC 4 cut(s) 793, 1502, 1712, 2415
Eco31I GGTCTC 2 cut(s) 490, 1312
Eco47I GGWCC 5 cut(s) 733, 964, 1903, 2132, 2335
Eco57I CTGAAG 5 cut(s) 288, 1071, 1488, 1779, 2328
Eco88I CYCGRG 1 cut(s) 2127
EcoO109I RGGNCCY 4 cut(s) 733, 964, 2132, 2335
EcoRI GAATTC 2 cut(s) 1866, 2290
EcoRII CCWGG 7 cut(s) 1243, 1252, 1376, 1905, 1951, 1969, 2134
EcoT14I CCWWGG 3 cut(s) 1018, 1778, 2021
EcoT22I ATGCAT 1 cut(s) 678
EcoT38I GRGCYC 4 cut(s) 793, 1502, 1712, 2415
ErhI CCWWGG 3 cut(s) 1018, 1778, 2021
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FaqI GGGAC 6 cut(s) 746, 977, 1196, 2185, 2321, 2411
FauI CCCGC 2 cut(s) 2098, 2277
FauNDI CATATG 4 cut(s) 70, 1064, 1401, 2314
FbaI TGATCA 1 cut(s) 154
FblI GTMKAC 1 cut(s) 2264
Fnu4HI GCNGC 7 cut(s) 175, 216, 396, 399, 471, 1412, 2319
FokI GGATG 4 cut(s) 16, 1561, 1580, 1785
FriOI GRGCYC 4 cut(s) 793, 1502, 1712, 2415
Fsp4HI GCNGC 7 cut(s) 175, 216, 396, 399, 471, 1412, 2319
FspBI CTAG 6 cut(s) 39, 582, 1049, 1208, 1596, 1748
GlaI GCGC 4 cut(s) 1115, 1147, 1481, 2247
GluI GCNGC 7 cut(s) 175, 216, 396, 399, 471, 1412, 2319
GsaI CCCAGC 1 cut(s) 24
GsuI CTGGAG 1 cut(s) 1983
HaeII RGCGCY 2 cut(s) 1149, 2249
HaeIII GGCC 5 cut(s) 206, 645, 1102, 1157, 1969
HapII CCGG 3 cut(s) 482, 1158, 1578
HgaI GACGC 1 cut(s) 132
HhaI GCGC 4 cut(s) 1116, 1148, 1482, 2248
Hin6I GCGC 4 cut(s) 1114, 1146, 1480, 2246
HinP1I GCGC 4 cut(s) 1114, 1146, 1480, 2246
HincII GTYRAC 1 cut(s) 1882
HindII GTYRAC 1 cut(s) 1882
HindIII AAGCTT 1 cut(s) 2030
HpaI GTTAAC 1 cut(s) 1882
HpaII CCGG 3 cut(s) 482, 1158, 1578
HphI GGTGA 5 cut(s) 149, 509, 2179, 2203, 2215
Hpy166II GTNNAC 7 cut(s) 434, 566, 898, 1365, 1561, 1882, 2265
Hpy188I TCNGA 7 cut(s) 154, 307, 340, 376, 838, 1125, 1824
Hpy8I GTNNAC 7 cut(s) 434, 566, 898, 1365, 1561, 1882, 2265
HpyAV CCTTC 9 cut(s) 127, 709, 1190, 1281, 1701, 1762, 1802, 2049, 2230
HpyCH4III ACNGT 7 cut(s) 127, 1041, 1345, 1369, 1681, 1819, 2102
HpyCH4IV ACGT 4 cut(s) 568, 935, 2237, 2274
HpyF10VI GCNNNNNNNGC 7 cut(s) 212, 272, 476, 819, 1154, 1946, 2318
HpySE526I ACGT 4 cut(s) 568, 935, 2237, 2274
HspAI GCGC 4 cut(s) 1114, 1146, 1480, 2246
KflI GGGWCCC 2 cut(s) 733, 2335
Kpn2I TCCGGA 1 cut(s) 1577
Ksp22I TGATCA 1 cut(s) 154
KspAI GTTAAC 1 cut(s) 1882
LguI GCTCTTC 1 cut(s) 1323
LmnI GCTCC 4 cut(s) 171, 484, 1855, 1954
Lsp1109I GCAGC 4 cut(s) 202, 382, 482, 2305
LweI GCATC 7 cut(s) 73, 146, 202, 253, 866, 1562, 1927
MabI ACCWGGT 1 cut(s) 2134
MaeI CTAG 6 cut(s) 39, 582, 1049, 1208, 1596, 1748
MaeII ACGT 4 cut(s) 568, 935, 2237, 2274
MaeIII GTNAC 2 cut(s) 382, 2345
MbiI CCGCTC 1 cut(s) 2284
MflI RGATCY 7 cut(s) 149, 460, 830, 859, 1199, 1824, 1997
MhlI GDGCHC 7 cut(s) 793, 900, 919, 1502, 1712, 1852, 2415
MlyI GAGTC 3 cut(s) 248, 1012, 2393
Mph1103I ATGCAT 1 cut(s) 678
MroI TCCGGA 1 cut(s) 1577
MslI CAYNNNNRTG 5 cut(s) 596, 621, 681, 888, 1803
MspI CCGG 3 cut(s) 482, 1158, 1578
MspR9I CCNGG 7 cut(s) 1245, 1254, 1378, 1907, 1953, 1971, 2136
Mva1269I GAATGC 3 cut(s) 370, 883, 1350
MvaI CCWGG 7 cut(s) 1245, 1254, 1378, 1907, 1953, 1971, 2136
MvnI CGCG 2 cut(s) 177, 1482
MwoI GCNNNNNNNGC 7 cut(s) 212, 272, 476, 819, 1154, 1946, 2318
NcoI CCATGG 2 cut(s) 1778, 2021
NdeI CATATG 4 cut(s) 70, 1064, 1401, 2314
NlaIV GGNNCC 6 cut(s) 173, 734, 735, 965, 2336, 2337
NmuCI GTSAC 1 cut(s) 382
NsiI ATGCAT 1 cut(s) 678
NspI RCATGY 1 cut(s) 1295
PaqCI CACCTGC 1 cut(s) 2155
PciSI GCTCTTC 1 cut(s) 1323
PcsI WCGNNNNNNNCGW 2 cut(s) 1861, 2204
PctI GAATGC 3 cut(s) 370, 883, 1350
PfeI GAWTC 8 cut(s) 500, 653, 803, 839, 905, 1082, 1646, 2170
PflFI GACNNNGTC 1 cut(s) 380
PflMI CCANNNNNTGG 1 cut(s) 743
PfoI TCCNGGA 3 cut(s) 1243, 1252, 1905
PkrI GCNGC 7 cut(s) 176, 217, 397, 400, 472, 1413, 2320
PleI GAGTC 3 cut(s) 247, 1011, 2393
PpsI GAGTC 3 cut(s) 247, 1011, 2393
Ppu21I YACGTR 1 cut(s) 569
PpuMI RGGWCCY 4 cut(s) 733, 964, 2132, 2335
PshAI GACNNNNGTC 1 cut(s) 1304
PsiI TTATAA 1 cut(s) 1221
Psp1406I AACGTT 1 cut(s) 935
Psp5II RGGWCCY 4 cut(s) 733, 964, 2132, 2335
Psp6I CCWGG 7 cut(s) 1243, 1252, 1376, 1905, 1951, 1969, 2134
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 7 cut(s) 1243, 1252, 1376, 1905, 1951, 1969, 2134
PspN4I GGNNCC 6 cut(s) 173, 734, 735, 965, 2336, 2337
PspPI GGNCC 6 cut(s) 733, 964, 1903, 1967, 2132, 2335
PspPPI RGGWCCY 4 cut(s) 733, 964, 2132, 2335
PstNI CAGNNNCTG 2 cut(s) 146, 908
PsuI RGATCY 7 cut(s) 149, 460, 830, 859, 1199, 1824, 1997
PsyI GACNNNGTC 1 cut(s) 380
RsaI GTAC 7 cut(s) 352, 571, 928, 977, 1398, 2273, 2378
RsaNI GTAC 7 cut(s) 351, 570, 927, 976, 1397, 2272, 2377
RseI CAYNNNNRTG 5 cut(s) 596, 621, 681, 888, 1803
SapI GCTCTTC 1 cut(s) 1323
SatI GCNGC 7 cut(s) 175, 216, 396, 399, 471, 1412, 2319
Sau96I GGNCC 6 cut(s) 733, 964, 1903, 1967, 2132, 2335
SchI GAGTC 3 cut(s) 248, 1012, 2393
ScrFI CCNGG 7 cut(s) 1245, 1254, 1378, 1907, 1953, 1971, 2136
SduI GDGCHC 7 cut(s) 793, 900, 919, 1502, 1712, 1852, 2415
SexAI ACCWGGT 1 cut(s) 2134
SfaNI GCATC 7 cut(s) 73, 146, 202, 253, 866, 1562, 1927
SfcI CTRYAG 2 cut(s) 1037, 2043
SinI GGWCC 5 cut(s) 733, 964, 1903, 2132, 2335
SmiMI CAYNNNNRTG 5 cut(s) 596, 621, 681, 888, 1803
SmlI CTYRAG 3 cut(s) 317, 1174, 1612
SmoI CTYRAG 3 cut(s) 317, 1174, 1612
SnaBI TACGTA 1 cut(s) 569
SsiI CCGC 6 cut(s) 175, 399, 893, 1412, 2105, 2284
SspI AATATT 3 cut(s) 418, 1275, 1453
SspMI CTAG 6 cut(s) 39, 582, 1049, 1208, 1596, 1748
StyD4I CCNGG 7 cut(s) 1243, 1252, 1376, 1905, 1951, 1969, 2134
StyI CCWWGG 3 cut(s) 1018, 1778, 2021
TaaI ACNGT 7 cut(s) 127, 1041, 1345, 1369, 1681, 1819, 2102
TaiI ACGT 4 cut(s) 571, 938, 2240, 2277
TaqI TCGA 2 cut(s) 465, 749
TaqII GACCGA 1 cut(s) 1995
TatI WGTACW 3 cut(s) 975, 1396, 2376
TauI GCSGC 3 cut(s) 177, 401, 1414
TfiI GAWTC 8 cut(s) 500, 653, 803, 839, 905, 1082, 1646, 2170
TscAI CASTG 6 cut(s) 672, 1372, 1563, 1708, 1747, 2107
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 4 cut(s) 215, 395, 470, 2318
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 4 cut(s) 442, 773, 1284, 1859
TspRI CASTG 6 cut(s) 672, 1372, 1563, 1708, 1747, 2107
Tth111I GACNNNGTC 1 cut(s) 380
Van91I CCANNNNNTGG 1 cut(s) 743
VneI GTGCAC 1 cut(s) 896
VpaK11BI GGWCC 5 cut(s) 733, 964, 1903, 2132, 2335
XapI RAATTY 4 cut(s) 1162, 1670, 1866, 2290
XbaI TCTAGA 1 cut(s) 1048
XceI RCATGY 1 cut(s) 1295
XcmI CCANNNNNNNNNTGG 1 cut(s) 1039
XmiI GTMKAC 1 cut(s) 2264
XspI CTAG 6 cut(s) 39, 582, 1049, 1208, 1596, 1748
Zsp2I ATGCAT 1 cut(s) 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.