Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007420

Physical Location & Seq

Genomic Coordinates Reverse (-)

30939401 .. 30947428

Gene Structure 8028 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1233700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2331 bp

ATGGAAATTCTGAGACTAATCGAAGTTGTATTGGTTGTTTATGCGATGCACCGAGTGGGTAGTTCAGGGAACACAAACAATTCCACTCGCCCTCGTAAAGAGAAGAGATTAACATATGTGTTGAGTGATGCCAACGACACGAAGCATTGTGCCGGCATAAATTCTTTGTCTGTACTTAAGGGATCAGTGTCTGGGTGTGATTACCTGTTTACTGGAAGCCGTGATGGCACACTAAAAAGATGGACGCTGGCCGAAGATGCTGCTACGTGTTCAGCAACCTTTGAGTCTCATGTGGATTGGGTTAATGATGCTGTTCTTGCAGGTTATAATACGCTTGTTTCTTGTTCTTCAGATACCACCATTAAGATGTGGAATTGCATGTCAGACGGAGCTTGTACCAAAACTCTTCGTCAGCATTCTGACTATGTCACATGCCTTGCTTCCGCAGAGAAAAATAGCAATATTGTTGCCTCTGGTGGACTTGGAGGGGAGGTTTTTGTATGGGATCTTGAAGCAGCTCTTACTCCAGTTGCAAAGTCAGGTGAATCAATGGAAGATGATAGTTCAAATGCTGTCAATGGTTCTGGAAATGCAATTACAAGTTTACGTACTATCAGCTCTAGCAACTGCATTTCTATGCACACAACTCAGCCACATGGATATGTTCCAATCTCTGCTAAAGGTCATAAGGAATCAGTTTATGCATTGGCAATGAATGATAGTGGATCGCTTCTGGTCTCTGGTGGAACAGAGAAGGTTGTTCGTGTTTGGGATCCAAGAACAGGTTCAAAGAAAATGAAACTAAGAGGGCATACAGATAATATCAGGGCTCTGCTGATTGATTCTACTGGAAGATTTTGCTTATCAGGATCTTCTGATTCAATGATCAGGCTATGGGATCTTGGTCAGCAGCGCTGTGTGCATTCTTATGCTGTGCATACAGATTCTGTTTGGGCACTTGCCAGTACCCCAACATTTAGTCATGTATATAGTGGTGGGAGAGATCTTTCTTTATACTTGACAGACTTGGCCACAAGAGAGAGTCTCTTGCTTTCCACAGGGGAACATCCCATTCTGCAGTTAGCTTTACAAGATGATAGTATATGGGTTGCAACAACAGATTCTTCAGTTCATAGATGGCCTGCTGAAGGGAGTAATCCCCAGAAGATAATTCAAAGAGGTGGTGCATTCTTGGCTGGAAATTTGTCCTTTTCAAGAGCAAGGGTGTCATTAGAAGGATCAACCCTAGTCCCTGTTAATAAAGAACCTATTTTTACGATTCCTGGAATTCCTGGAATAGTGCAACATGAAATATTAAACAATAGAAGGCATGTCTTGACAAGGGACACTGCTGATTCAGTGAAGTTGTGGGAGATTACGAGGGGTGTTGTCATTGAGGATTATGGAAAGGTCAAATTTGAGGAGAAAAAGGAAGAGCTTTTTGAGATGGTGAGCATTCCTGCATGGTTCACTGTGGATACCAGACTTGGAAGTTTATCCGTACATTTAGATACCCCACAATGCTTTTCGGCAGAGATGTATTCAGCAGACCTGAACATTGTGGGGAAGCCTGAAGATGACAAGGTTAATCTAGCACGAGAAACCCTTAAAGGGCTCTTGGCTCATTGGTTGGGGAAAAGAAAGCAAAGATTTGGATCACAAGCATCAGCTAATGGGGATGTGTCATCCGGAAAGGATATTACTACTAGAAGTATTACCCATTCAAGAATTGAGGTGGATAGTAATAATGAAAATGATTCAATGGTCTATCCTCCCTTCGAATTTTCTACAACTTCCCCTCCTTCTATTATTACTGAGGGCTCTCAGGGGGGACCATGGAGGAAGAAAATCACTGACTTAGATGGTACTGAAGATGAGAAGGACTTTCCCTGGTGGTGTCTCGATTGCGTGTTGAATAATCGCCTACCCCCTAGAGAAAATACCAAGTGCAGTTTTTATCTACATCCATGTGAAGGTTCAACTGTCCAGATCCTCACACAAGGGAAACTAAGCGCCCCTCGTATTTTACGAATCCATAAAGTAGTTAATTATGTTGTTGAGAAGATGGTCCTTGACAAACCATTGGATAGTGCCAATCCTGATGCAACATTTGCTCCTGGTCTTGCTGGAGGGCCAGTGCAGCTTTCATCGGTAGGAGATGGATCCTTCCGATCTGGATTGAAGCAATGGCAAAAGCTTAAGCCTTCTATAGAGATATTGTGTAACAATCAGGTCTTGTCCTCAGACATGAGCTTAGCCACGGTGCGGGCTTATATATGGAAGAAATCTGAGGATCTGATTCTCAATTACAGGGTGATTCAGGGCAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016579 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0070646 GO:0070647 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03460 map03460

Pfam Domains

Protein Families

DUF3337 WD40

Protein Analysis

777

Amino Acids

85.09

Weight (kDa)

6.48

Isoelectric Point (pI)

41.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_WDHD1_1st	PF24817	43 - 172	5.9e-07	WDHD1 first WD40 domain
WDR55	PF24796	46 - 169	2.3e-06	WDR55
WD40_Prp19	PF24814	51 - 175	1.2e-10	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	55 - 172	5.5e-17	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	66 - 160	1.8e-06	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	68 - 170	7.1e-12	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	68 - 175	5.2e-12	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	69 - 178	4.4e-08	TEP-1 second beta-propeller
WD40_CDC20-Fz	PF24807	135 - 278	3e-13	CDC20/Fizzy WD40 domain
Beta-prop_EML	PF23409	138 - 377	3.9e-09	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st	PF25173	165 - 338	1e-26	WDR3 first beta-propeller domain
WDR55	PF24796	201 - 305	8.8e-10	WDR55
EIF3I	PF24805	223 - 314	1.4e-10	EIF3I
Beta-prop_WDR3_2nd	PF25172	225 - 300	1.1e-09	WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	226 - 362	2e-08	TEP-1 second beta-propeller
WD40_Gbeta	PF25391	226 - 353	1.2e-10	G protein beta WD-40 repeat protein
Beta-prop_WDR5	PF25175	226 - 392	2.2e-20	WDR5 beta-propeller domain
Beta-prop_WDR41	PF25178	226 - 343	1e-06	WDR41
Beta-prop_CAF1B_HIR1	PF24105	227 - 310	6.4e-08	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	229 - 387	9.8e-22	THOC3 beta-propeller domain
WD40_Prp19	PF24814	230 - 380	2.7e-18	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	231 - 313	2.2e-12	WDHD1 first WD40 domain
WD40	PF00400	264 - 300	4.1e-07	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	267 - 380	5.2e-06	WDR3 second beta-propeller domain
DUF3337	PF11816	625 - 771	2.6e-29	Domain of unknown function (DUF3337)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007420)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05090 AT3G05090 AT3G05090
fragaria_vesca	FvH4_6g03981
malus_domestica	MD04G1217000.v1.1 MD12G1233700.v1.1
prunus_persica	Prupe.6G336900_v2.0.a1 Prupe.6G336900_v2.0.a1 Prupe.6G336900_v2.0.a1 Prupe.6G336900_v2.0.a1 Prupe.6G336900_v2.0.a1 Prupe.6G336900_v2.0.a1 Prupe.6G336900_v2.0.a1 Prupe.6G336900_v2.0.a1
pyrus_communis	pycom04g19310
rosa_chinensis	RchiOBHm_Chr3g0452571
rosa_laevigata	RLG00000025583
rosa_multiflora	Rmu_sc0000608.1_g000022
rosa_roxburghii	Rroxscaffold_6G00429610
rosa_rugosa	Rorug02G0641300
rosa_samantha	Rh3AG043900 Rh3BG046000 Rh3CG044700 Rh3DG045000
rosa_wichuraiana	Rw3G003400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	327
AarI	CACCTGC	1 cut(s)	2316
Acc36I	ACCTGC	2 cut(s)	311, 2316
AccIII	TCCGGA	1 cut(s)	1688
AciI	CCGC	2 cut(s)	444, 2266
AcoI	YGGCCR	2 cut(s)	249, 1029
AcsI	RAATTY	6 cut(s)	6, 160, 1201, 1287, 1415, 1781
AcuI	CTGAAG	5 cut(s)	333, 1110, 1167, 1593, 1890
AdeI	CACNNNGTG	1 cut(s)	55
AfaI	GTAC	6 cut(s)	174, 397, 610, 967, 1503, 1867
AfeI	AGCGCT	1 cut(s)	914
AfiI	CCNNNNNNNGG	5 cut(s)	782, 1148, 1611, 1973, 2265
AflII	CTTAAG	2 cut(s)	176, 2198
AflIII	ACRYGT	1 cut(s)	266
AjnI	CCWGG	4 cut(s)	1282, 1291, 1889, 2116
AjuI	GAANNNNNNNTTGG	2 cut(s)	769, 801
AluBI	AGCT	9 cut(s)	392, 518, 618, 1085, 1438, 1670, 2143, 2197, 2253
AluI	AGCT	9 cut(s)	392, 518, 618, 1085, 1438, 1670, 2143, 2197, 2253
Alw26I	GTCTC	5 cut(s)	7, 291, 742, 1049, 1904
AlwNI	CAGNNNCTG	2 cut(s)	191, 947
Aor13HI	TCCGGA	1 cut(s)	1688
Aor51HI	AGCGCT	1 cut(s)	914
AoxI	GGCC	4 cut(s)	249, 1029, 1139, 2132
ApeKI	GCWGC	4 cut(s)	260, 515, 910, 2140
ApoI	RAATTY	6 cut(s)	6, 160, 1201, 1287, 1415, 1781
Asp700I	GAANNNNTTC	1 cut(s)	784
AspLEI	GCGC	2 cut(s)	915, 2015
AspS9I	GGNCC	3 cut(s)	1832, 2068, 2132
AsuHPI	GGTGA	3 cut(s)	554, 1462, 2326
AsuII	TTCGAA	1 cut(s)	1779
AvaII	GGWCC	2 cut(s)	1832, 2068
BaeGI	GKGCMC	1 cut(s)	958
BalI	TGGCCA	1 cut(s)	1031
BamHI	GGATCC	2 cut(s)	772, 2162
BanII	GRGCYC	3 cut(s)	832, 1617, 1823
BauI	CACGAG	1 cut(s)	1596
BbvI	GCAGC	4 cut(s)	247, 527, 922, 2152
BccI	CCATC	7 cut(s)	218, 234, 1131, 1441, 1856, 2059, 2153
BceAI	ACGGC	1 cut(s)	204
BcgI	CGANNNNNNTGC	2 cut(s)	242, 276
BciT130I	CCWGG	4 cut(s)	1284, 1293, 1891, 2118
BciVI	GTATCC	1 cut(s)	1471
BclI	TGATCA	1 cut(s)	885
BcoDI	GTCTC	5 cut(s)	7, 291, 742, 1049, 1904
BfaI	CTAG	5 cut(s)	621, 1247, 1592, 1707, 1932
BfmI	CTRYAG	2 cut(s)	1076, 2208
BfoI	RGCGCY	2 cut(s)	916, 2016
BfrI	CTTAAG	2 cut(s)	176, 2198
BfuAI	ACCTGC	2 cut(s)	311, 2316
BfuI	GTATCC	1 cut(s)	1471
BglI	GCCNNNNNGGC	1 cut(s)	225
BglII	AGATCT	1 cut(s)	1003
BisI	GCNGC	4 cut(s)	261, 516, 911, 2141
BlpI	GCTNAGC	1 cut(s)	2254
BlsI	GCNGC	4 cut(s)	262, 517, 912, 2142
Bme1390I	CCNGG	4 cut(s)	1284, 1293, 1891, 2118
Bme18I	GGWCC	2 cut(s)	1832, 2068
BmgT120I	GGNCC	3 cut(s)	1832, 2068, 2132
BmiI	GGNNCC	3 cut(s)	774, 1833, 2164
BmrFI	CCNGG	4 cut(s)	1284, 1293, 1891, 2118
BmsI	GCATC	6 cut(s)	36, 118, 247, 298, 1673, 2092
BplI	GAGNNNNNCTC	2 cut(s)	1029, 1061
BpmI	CTGGAG	2 cut(s)	510, 2148
Bpu1102I	GCTNAGC	1 cut(s)	2254
Bpu14I	TTCGAA	1 cut(s)	1779
BsaAI	YACGTR	2 cut(s)	267, 608
BsaBI	GATNNNNATC	1 cut(s)	1654
BsaI	GGTCTC	1 cut(s)	742
BsaJI	CCNNGG	3 cut(s)	1835, 1889, 2259
BsaWI	WCCGGW	1 cut(s)	1688
BsaXI	ACNNNNNCTCC	8 cut(s)	477, 507, 1783, 1813, 2098, 2121, 2128, 2151
Bsc4I	CCNNNNNNNGG	5 cut(s)	782, 1148, 1611, 1973, 2265
Bse118I	RCCGGY	1 cut(s)	152
Bse1I	ACTGG	5 cut(s)	217, 527, 853, 963, 2135
Bse3DI	GCAATG	2 cut(s)	717, 2192
Bse8I	GATNNNNATC	1 cut(s)	1654
BseAI	TCCGGA	1 cut(s)	1688
BseBI	CCWGG	4 cut(s)	1284, 1293, 1891, 2118
BseDI	CCNNGG	3 cut(s)	1835, 1889, 2259
BseGI	GGATG	4 cut(s)	1066, 1684, 1685, 1963
BseJI	GATNNNNATC	1 cut(s)	1654
BseLI	CCNNNNNNNGG	5 cut(s)	782, 1148, 1611, 1973, 2265
BseMI	GCAATG	2 cut(s)	717, 2192
BseMII	CTCAG	5 cut(s)	662, 1806, 1838, 2256, 2280
BseNI	ACTGG	5 cut(s)	217, 527, 853, 963, 2135
BseRI	GAGGAG	1 cut(s)	1436
BseSI	GKGCMC	1 cut(s)	958
BseXI	GCAGC	4 cut(s)	247, 527, 922, 2152
BsgI	GTGCAG	2 cut(s)	1969, 2159
BshFI	GGCC	4 cut(s)	251, 1031, 1141, 2134
BsiSI	CCGG	2 cut(s)	153, 1689
BslFI	GGGAC	3 cut(s)	1235, 1358, 1845
BslI	CCNNNNNNNGG	5 cut(s)	782, 1148, 1611, 1973, 2265
BsmAI	GTCTC	5 cut(s)	7, 291, 742, 1049, 1904
BsmFI	GGGAC	3 cut(s)	1235, 1358, 1845
BsmI	GAATGC	4 cut(s)	415, 922, 1187, 1455
BsnI	GGCC	4 cut(s)	251, 1031, 1141, 2134
Bso31I	GGTCTC	1 cut(s)	742
Bsp119I	TTCGAA	1 cut(s)	1779
Bsp1286I	GDGCHC	4 cut(s)	832, 958, 1617, 1823
Bsp13I	TCCGGA	1 cut(s)	1688
Bsp1720I	GCTNAGC	1 cut(s)	2254
Bsp19I	CCATGG	1 cut(s)	1835
BspACI	CCGC	2 cut(s)	444, 2266
BspANI	GGCC	4 cut(s)	251, 1031, 1141, 2134
BspCNI	CTCAG	5 cut(s)	661, 1807, 1837, 2255, 2281
BspEI	TCCGGA	1 cut(s)	1688
BspLI	GGNNCC	3 cut(s)	774, 1833, 2164
BspMAI	CTGCAG	1 cut(s)	1080
BspMI	ACCTGC	2 cut(s)	311, 2316
BspQI	GCTCTTC	1 cut(s)	1428
BspT104I	TTCGAA	1 cut(s)	1779
BspTI	CTTAAG	2 cut(s)	176, 2198
BspTNI	GGTCTC	1 cut(s)	742
BsrDI	GCAATG	2 cut(s)	717, 2192
BsrFI	RCCGGY	1 cut(s)	152
BsrI	ACTGG	5 cut(s)	217, 527, 853, 963, 2135
BssAI	RCCGGY	1 cut(s)	152
BssECI	CCNNGG	3 cut(s)	1835, 1889, 2259
BssSI	CACGAG	1 cut(s)	1596
BssT1I	CCWWGG	1 cut(s)	1835
Bst2BI	CACGAG	1 cut(s)	1596
Bst2UI	CCWGG	4 cut(s)	1284, 1293, 1891, 2118
Bst4CI	ACNGT	3 cut(s)	1474, 1984, 2263
Bst6I	CTCTTC	3 cut(s)	98, 411, 1428
BstAFI	CTTAAG	2 cut(s)	176, 2198
BstAPI	GCANNNNNTGC	1 cut(s)	2111
BstBAI	YACGTR	2 cut(s)	267, 608
BstBI	TTCGAA	1 cut(s)	1779
BstC8I	GCNNGC	4 cut(s)	154, 249, 1143, 2268
BstDSI	CCRYGG	2 cut(s)	1835, 2259
BstENI	CCTNNNNNAGG	1 cut(s)	1146
BstF5I	GGATG	4 cut(s)	1066, 1684, 1685, 1963
BstH2I	RGCGCY	2 cut(s)	916, 2016
BstHHI	GCGC	2 cut(s)	915, 2015
BstMAI	GTCTC	5 cut(s)	7, 291, 742, 1049, 1904
BstMWI	GCNNNNNNNGC	7 cut(s)	225, 257, 317, 919, 1193, 2111, 2140
BstNI	CCWGG	4 cut(s)	1284, 1293, 1891, 2118
BstNSI	RCATGY	3 cut(s)	382, 435, 1334
BstSCI	CCNGG	4 cut(s)	1282, 1291, 1889, 2116
BstSFI	CTRYAG	2 cut(s)	1076, 2208
BstSLI	GKGCMC	1 cut(s)	958
BstSNI	TACGTA	1 cut(s)	608
BstV1I	GCAGC	4 cut(s)	247, 527, 922, 2152
BstX2I	RGATCY	8 cut(s)	505, 772, 869, 898, 1003, 1989, 2162, 2293
BstYI	RGATCY	8 cut(s)	505, 772, 869, 898, 1003, 1989, 2162, 2293
BsuI	GTATCC	1 cut(s)	1471
BsuRI	GGCC	4 cut(s)	251, 1031, 1141, 2134
BtgI	CCRYGG	2 cut(s)	1835, 2259
BtgZI	GCGATG	1 cut(s)	59
BtsCI	GGATG	4 cut(s)	1066, 1684, 1685, 1963
BtsI	GCAGTG	1 cut(s)	1347
BtsIMutI	CAGTG	6 cut(s)	192, 1347, 1365, 1470, 1851, 2142
BveI	ACCTGC	2 cut(s)	311, 2316
Cac8I	GCNNGC	4 cut(s)	154, 249, 1143, 2268
CaiI	CAGNNNCTG	2 cut(s)	191, 947
CfoI	GCGC	2 cut(s)	915, 2015
Cfr10I	RCCGGY	1 cut(s)	152
Cfr13I	GGNCC	3 cut(s)	1832, 2068, 2132
CseI	GACGC	1 cut(s)	253
Csp6I	GTAC	6 cut(s)	173, 396, 609, 966, 1502, 1866
CviQI	GTAC	6 cut(s)	173, 396, 609, 966, 1502, 1866
DraIII	CACNNNGTG	1 cut(s)	55
EaeI	YGGCCR	2 cut(s)	249, 1029
Eam1104I	CTCTTC	3 cut(s)	98, 411, 1428
EarI	CTCTTC	3 cut(s)	98, 411, 1428
Eco105I	TACGTA	1 cut(s)	608
Eco130I	CCWWGG	1 cut(s)	1835
Eco24I	GRGCYC	3 cut(s)	832, 1617, 1823
Eco31I	GGTCTC	1 cut(s)	742
Eco47I	GGWCC	2 cut(s)	1832, 2068
Eco47III	AGCGCT	1 cut(s)	914
Eco57I	CTGAAG	5 cut(s)	333, 1110, 1167, 1593, 1890
EcoNI	CCTNNNNNAGG	1 cut(s)	1146
EcoRI	GAATTC	1 cut(s)	1287
EcoRII	CCWGG	4 cut(s)	1282, 1291, 1889, 2116
EcoT14I	CCWWGG	1 cut(s)	1835
EcoT22I	ATGCAT	1 cut(s)	706
EcoT38I	GRGCYC	3 cut(s)	832, 1617, 1823
ErhI	CCWWGG	1 cut(s)	1835
FalI	AAGNNNNNCTT	2 cut(s)	504, 536
FaqI	GGGAC	3 cut(s)	1235, 1358, 1845
FauI	CCCGC	1 cut(s)	2259
FauNDI	CATATG	1 cut(s)	115
FbaI	TGATCA	1 cut(s)	885
Fnu4HI	GCNGC	4 cut(s)	261, 516, 911, 2141
FokI	GGATG	4 cut(s)	1053, 1672, 1691, 1950
FriOI	GRGCYC	3 cut(s)	832, 1617, 1823
Fsp4HI	GCNGC	4 cut(s)	261, 516, 911, 2141
FspBI	CTAG	5 cut(s)	621, 1247, 1592, 1707, 1932
GlaI	GCGC	2 cut(s)	914, 2014
GluI	GCNGC	4 cut(s)	261, 516, 911, 2141
GsuI	CTGGAG	2 cut(s)	510, 2148
HaeII	RGCGCY	2 cut(s)	916, 2016
HaeIII	GGCC	4 cut(s)	251, 1031, 1141, 2134
HapII	CCGG	2 cut(s)	153, 1689
HgaI	GACGC	1 cut(s)	253
HhaI	GCGC	2 cut(s)	915, 2015
Hin6I	GCGC	2 cut(s)	913, 2013
HinP1I	GCGC	2 cut(s)	913, 2013
HindIII	AAGCTT	1 cut(s)	2195
HpaII	CCGG	2 cut(s)	153, 1689
HphI	GGTGA	3 cut(s)	554, 1462, 2326
Hpy166II	GTNNAC	4 cut(s)	210, 479, 605, 1470
Hpy188I	TCNGA	9 cut(s)	12, 352, 385, 421, 877, 2171, 2245, 2290, 2298
Hpy8I	GTNNAC	4 cut(s)	210, 479, 605, 1470
HpyCH4III	ACNGT	3 cut(s)	1474, 1984, 2263
HpyCH4IV	ACGT	2 cut(s)	266, 607
HpyF10VI	GCNNNNNNNGC	7 cut(s)	225, 257, 317, 919, 1193, 2111, 2140
HpySE526I	ACGT	2 cut(s)	266, 607
HspAI	GCGC	2 cut(s)	913, 2013
Kpn2I	TCCGGA	1 cut(s)	1688
KroI	GCCGGC	1 cut(s)	152
KroNI	GCCGGC	1 cut(s)	154
Ksp22I	TGATCA	1 cut(s)	885
LguI	GCTCTTC	1 cut(s)	1428
LmnI	GCTCC	2 cut(s)	389, 2119
Lsp1109I	GCAGC	4 cut(s)	247, 527, 922, 2152
LweI	GCATC	6 cut(s)	36, 118, 247, 298, 1673, 2092
MaeI	CTAG	5 cut(s)	621, 1247, 1592, 1707, 1932
MaeII	ACGT	2 cut(s)	266, 607
MaeIII	GTNAC	2 cut(s)	427, 2222
MflI	RGATCY	8 cut(s)	505, 772, 869, 898, 1003, 1989, 2162, 2293
MhlI	GDGCHC	4 cut(s)	832, 958, 1617, 1823
MlsI	TGGCCA	1 cut(s)	1031
MluNI	TGGCCA	1 cut(s)	1031
MlyI	GAGTC	2 cut(s)	293, 1051
Mox20I	TGGCCA	1 cut(s)	1031
Mph1103I	ATGCAT	1 cut(s)	706
MroI	TCCGGA	1 cut(s)	1688
MroNI	GCCGGC	1 cut(s)	152
MroXI	GAANNNNTTC	1 cut(s)	784
MscI	TGGCCA	1 cut(s)	1031
MslI	CAYNNNNRTG	5 cut(s)	365, 635, 660, 927, 1968
Msp20I	TGGCCA	1 cut(s)	1031
MspCI	CTTAAG	2 cut(s)	176, 2198
MspI	CCGG	2 cut(s)	153, 1689
MspR9I	CCNGG	4 cut(s)	1284, 1293, 1891, 2118
Mva1269I	GAATGC	4 cut(s)	415, 922, 1187, 1455
MvaI	CCWGG	4 cut(s)	1284, 1293, 1891, 2118
MwoI	GCNNNNNNNGC	7 cut(s)	225, 257, 317, 919, 1193, 2111, 2140
NaeI	GCCGGC	1 cut(s)	154
NcoI	CCATGG	1 cut(s)	1835
NdeI	CATATG	1 cut(s)	115
NgoMIV	GCCGGC	1 cut(s)	152
NlaIV	GGNNCC	3 cut(s)	774, 1833, 2164
NmuCI	GTSAC	1 cut(s)	427
NsiI	ATGCAT	1 cut(s)	706
NspI	RCATGY	3 cut(s)	382, 435, 1334
NspV	TTCGAA	1 cut(s)	1779
PaqCI	CACCTGC	1 cut(s)	2316
PciSI	GCTCTTC	1 cut(s)	1428
PcsI	WCGNNNNNNNCGW	1 cut(s)	2026
PctI	GAATGC	4 cut(s)	415, 922, 1187, 1455
PdiI	GCCGGC	1 cut(s)	154
PdmI	GAANNNNTTC	1 cut(s)	784
PflFI	GACNNNGTC	1 cut(s)	425
PfoI	TCCNGGA	2 cut(s)	1282, 1291
PkrI	GCNGC	4 cut(s)	262, 517, 912, 2142
PleI	GAGTC	2 cut(s)	292, 1050
PpsI	GAGTC	2 cut(s)	292, 1050
Ppu21I	YACGTR	2 cut(s)	267, 608
PsiI	TTATAA	1 cut(s)	327
Psp6I	CCWGG	4 cut(s)	1282, 1291, 1889, 2116
PspGI	CCWGG	4 cut(s)	1282, 1291, 1889, 2116
PspN4I	GGNNCC	3 cut(s)	774, 1833, 2164
PspPI	GGNCC	3 cut(s)	1832, 2068, 2132
PstI	CTGCAG	1 cut(s)	1080
PstNI	CAGNNNCTG	2 cut(s)	191, 947
PsuI	RGATCY	8 cut(s)	505, 772, 869, 898, 1003, 1989, 2162, 2293
PsyI	GACNNNGTC	1 cut(s)	425
RsaI	GTAC	6 cut(s)	174, 397, 610, 967, 1503, 1867
RsaNI	GTAC	6 cut(s)	173, 396, 609, 966, 1502, 1866
RseI	CAYNNNNRTG	5 cut(s)	365, 635, 660, 927, 1968
SapI	GCTCTTC	1 cut(s)	1428
SatI	GCNGC	4 cut(s)	261, 516, 911, 2141
Sau96I	GGNCC	3 cut(s)	1832, 2068, 2132
SchI	GAGTC	2 cut(s)	293, 1051
ScrFI	CCNGG	4 cut(s)	1284, 1293, 1891, 2118
SduI	GDGCHC	4 cut(s)	832, 958, 1617, 1823
SfaNI	GCATC	6 cut(s)	36, 118, 247, 298, 1673, 2092
SfcI	CTRYAG	2 cut(s)	1076, 2208
SfuI	TTCGAA	1 cut(s)	1779
SinI	GGWCC	2 cut(s)	1832, 2068
SmiMI	CAYNNNNRTG	5 cut(s)	365, 635, 660, 927, 1968
SmlI	CTYRAG	2 cut(s)	176, 2198
SmoI	CTYRAG	2 cut(s)	176, 2198
SnaBI	TACGTA	1 cut(s)	608
SsiI	CCGC	2 cut(s)	444, 2266
SspI	AATATT	2 cut(s)	463, 1314
SspMI	CTAG	5 cut(s)	621, 1247, 1592, 1707, 1932
StyD4I	CCNGG	4 cut(s)	1282, 1291, 1889, 2116
StyI	CCWWGG	1 cut(s)	1835
TaaI	ACNGT	3 cut(s)	1474, 1984, 2263
TaiI	ACGT	2 cut(s)	269, 610
TaqI	TCGA	3 cut(s)	21, 1779, 1902
TatI	WGTACW	1 cut(s)	172
TscAI	CASTG	6 cut(s)	192, 1354, 1365, 1477, 1858, 2142
TseFI	GTSAC	1 cut(s)	427
TseI	GCWGC	4 cut(s)	260, 515, 910, 2140
Tsp45I	GTSAC	1 cut(s)	427
TspDTI	ATGAA	6 cut(s)	728, 812, 1121, 1323, 1764, 2136
TspGWI	ACGGA	2 cut(s)	402, 1489
TspRI	CASTG	6 cut(s)	192, 1354, 1365, 1477, 1858, 2142
Tth111I	GACNNNGTC	1 cut(s)	425
Vha464I	CTTAAG	2 cut(s)	176, 2198
VpaK11BI	GGWCC	2 cut(s)	1832, 2068
XagI	CCTNNNNNAGG	1 cut(s)	1146
XapI	RAATTY	6 cut(s)	6, 160, 1201, 1287, 1415, 1781
XceI	RCATGY	3 cut(s)	382, 435, 1334
XmnI	GAANNNNTTC	1 cut(s)	784
XspI	CTAG	5 cut(s)	621, 1247, 1592, 1707, 1932
Zsp2I	ATGCAT	1 cut(s)	706

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.