RLG00000025583

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0007420
Physical Location & Seq
Reverse (-)
46860338 .. 46867574
7237 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025583

Sequence Viewer

Length: 2301 bp
ATGTACTTATTGGCATACTTAATAACTGATCGAAGTTTGGTTTCAGTTGTAGCTGCATTAGTTGTTTCTGCAATGCACCGAGTGGGTAGTGCTGGGAACACATCCAATTCTAGTCGCCCTCGTAAAGAGAAGAGATTAACATATGTATTGAATGATGCTGATGACAAAAAGCATTGTGCAGGCATAAATTGTTTGACTGTATTGAAGGCATCAGCGTCTGGATCTGATCACCTATTTACTGGGAGCCGCGATGGAACACTAAAAAGATGGGCTGTGGCCGAAGATGCCGCTACCTGCTCAGCAACCTTCGAGTCTCATGTGGATTGGTACTTCTTGACTGCATGCATAAATGGAACTTGTACCAGAACTCTTCGTCAGCATTCTGACTATGTCACTTGTCTTGCTACCGCAGAAAAAAATAGCAATATTGTTGCCTCTGGTGGACTTGGAGGGGAGGTATTCATTTGGGATCTCGAAGCAGCCCTTGCTCCGGTCTCAAAGTCAGGTGATTCTGTGGAAGATGATTGTTCAAATGCCACCAATGGTTCTGGAAATGCACAACCGATTACAAGTTTACGTACTATCAGCTCTAGCAACAGCATTTCTATGCACTCAACTCAGGCACATGGATATGTTCCAATCTCTGCCAAAGGCCATAAAGAATCAGTTTATGCACTGGCAATGCATGATAGTGGATCACTTCTTGTCTCTGGTGGAACAGAGAAGGTCGTGCGTGTTTGGGACCCAAGAAGTGGCTCGAAGACTATGAAACTAAGAGGGCATACAGACAATATCAGGGCTCTGCTTCTTGATTCTACTGGCAGGTTTTGCTTGTCAGGATCTTCTGATTCAATGATTAGACTATGGGATCTTGGTCAGCAAAGATGCGTGCATTCTTATGCGGTGCACACAGATTCTGTTTGGGCACTTGCCAGTACGCCAACGTTTAGTCATGTATATAGTGGTGGGAGAGACCTTTCTTTGTACTTGACAGACTTGACCACGAGAGAGAGTCTTTTGCTTTGCCAAGGAGAACACCCCATTCTGCAGTTGGCTCTAGATGATGATAGCATATGGGTTGCAACAACAGATTCTTCAGTTAATAGATGGCCTGCTGAGGGGCGCAATCCTCAGAAAATTATCCAAAGAGGTGGAGCTTTCTTGGCTGGAAATTTGTCATTCTCAAGAGCAAGGGTGTCCTTGGAAGGATCTACCCTAGTCCCTGTTTATAAAGAGCCAGTCTTTACAATTCCTGGAACTCCTGGAATAGTGCAACATGAAATATTAAATAATAGAAGGCATGTCTTGACAAAGGATACTGCTTCTTCGGTGAAGCTGTGGGAGATTACTAGAGGTGTTGTTATTGAGGATTACGGAAAGGTCCCGTTTGAGGAGAAAAAGGAAGAGCTTTTTGAGACTGTAAGCATTCCTGCATGGTTCACTGTGGATACCAGGCTTGGAAGTTTATCTGTACATATGGATACCCCACAATGCTTTTCAGCAGAGATGTATTCGGCAGACCTTAATATTGTAGGAAAGCCTGAAGATGACAAGGTTAATCTAGCGCGAGAAACGCTTAAAGGGCTCTTGGCTCATTGGTTGGGGAAAAGAAAGCAAAGATTGGGAACACAAGCATCAGTGAACGGGGATGTGTCATCCGGAAAGGAAATCACTCCTAGAAGTATTACCCACTCAAGAATGGAGGTGGATAGTAATGCCGAAAATGATTCAATGGTTTATCCTCCCTTTGAATTTTCTACAGTTTCCCCTCCTTCTATCATCACTGAGGGCTCTCAGGGAGGACCATGGAGAAAGAAGATCACTGACCTGGATGGAACTGAAGATGAGAAGGACTTTCCATGGTGGTGTCTGGATTGTGTGTTGAATAATCGCCTACCTCCTAGAGAAAATACCAAGTGCAGTTTTTATCTACATCCATGTGAAGGTTCAACTGTTCAGATCCTCACACAAGGAAAACTAAGTGCACCTCGTATATTACGAATTCATAAGGTTGTCAACTATGTAGTTGAAAAGATGGTCCTGGACAAACCATTAGATAGTGCAAATCCTGATGCAACATTTGCTCCTGGTCTTGCTGGAGGGCCAGGACAACTCTCTGGCATTGGAGATGGATCTTTTCGGTCTGGATTGAAGCCATGGCAGAAGCTTAAACCGTCTATAGAGATATTGTGCAACAATCAGGTCTTGTCGCCAGAAATGAGCTTAGCCACAGTGCGGGCTTATGTATGGAAGAAACCCGAGGACCTGGTTCTCAATTACAGGGTGGTTCAGGGCAGGTGA

Protein Analysis

767

Amino Acids

83.75

Weight (kDa)

6.73

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 57 - 160 3.1e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 76 - 352 5.2e-25 THOC3 beta-propeller domain
WD40_Prp19 PF24814 118 - 268 1.7e-10 Prp19 WD40 domain
WD40_Gbeta PF25391 119 - 343 4.2e-12 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 123 - 268 6.1e-13 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 125 - 366 2.7e-08 Echinoderm microtubule-associated protein first beta-propeller
WDR55 PF24796 191 - 296 4.5e-11 WDR55
Beta-prop_WDR3_1st PF25173 197 - 328 1e-26 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 210 - 290 1e-09 WDR3 second beta-propeller domain
EIF3I PF24805 213 - 304 1.3e-09 EIF3I
Beta-prop_TEP1_2nd PF25047 216 - 338 1.1e-07 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 216 - 299 5.3e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR41 PF25178 216 - 333 5e-06 WDR41
WD40 PF00400 217 - 248 5e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 217 - 371 5.1e-20 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 218 - 306 3.8e-06 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 220 - 345 7.4e-19 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 220 - 302 4e-12 WDHD1 first WD40 domain
WD40 PF00400 254 - 290 4.8e-07 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 256 - 370 7.8e-06 WDR3 second beta-propeller domain
DUF3337 PF11816 615 - 761 9.8e-29 Domain of unknown function (DUF3337)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1230
AarI CACCTGC 1 cut(s) 2286
Acc36I ACCTGC 3 cut(s) 302, 813, 2286
AccB7I CCANNNNNTGG 1 cut(s) 752
AccII CGCG 2 cut(s) 249, 1567
AccIII TCCGGA 1 cut(s) 1658
AciI CCGC 5 cut(s) 247, 288, 408, 902, 2236
AclI AACGTT 1 cut(s) 944
AclWI GGATC 8 cut(s) 229, 477, 703, 847, 876, 1216, 1954, 2140
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 3 cut(s) 1171, 1751, 2001
AcuI CTGAAG 3 cut(s) 1080, 1563, 1860
AdeI CACNNNGTG 1 cut(s) 82
AfaI GTAC 7 cut(s) 5, 329, 361, 580, 937, 986, 1473
AfiI CCNNNNNNNGG 6 cut(s) 490, 752, 1118, 1390, 1943, 2235
AjnI CCWGG 8 cut(s) 1252, 1261, 1451, 1827, 2040, 2086, 2104, 2265
AluBI AGCT 7 cut(s) 53, 588, 1157, 1336, 1408, 2167, 2223
AluI AGCT 7 cut(s) 53, 588, 1157, 1336, 1408, 2167, 2223
Alw21I GWGCWC 2 cut(s) 909, 1987
Alw26I GTCTC 5 cut(s) 318, 499, 712, 966, 1409
Alw44I GTGCAC 2 cut(s) 905, 1983
AlwI GGATC 8 cut(s) 229, 477, 703, 847, 876, 1216, 1954, 2140
AlwNI CAGNNNCTG 2 cut(s) 218, 917
Ama87I CYCGRG 1 cut(s) 2258
Aor13HI TCCGGA 1 cut(s) 1658
AoxI GGCC 4 cut(s) 276, 652, 1109, 2102
ApaLI GTGCAC 2 cut(s) 905, 1983
ApeKI GCWGC 2 cut(s) 53, 479
ApoI RAATTY 3 cut(s) 1171, 1751, 2001
AspLEI GCGC 2 cut(s) 1125, 1567
AspS9I GGNCC 6 cut(s) 742, 1381, 1802, 2038, 2102, 2263
AsuHPI GGTGA 3 cut(s) 221, 518, 1342
AvaI CYCGRG 1 cut(s) 2258
AvaII GGWCC 5 cut(s) 742, 1381, 1802, 2038, 2263
BaeGI GKGCMC 3 cut(s) 909, 928, 1987
BanII GRGCYC 3 cut(s) 802, 1587, 1793
BauI CACGAG 1 cut(s) 1003
BbsI GAAGAC 1 cut(s) 767
Bbv12I GWGCWC 2 cut(s) 909, 1987
BbvCI CCTCAGC 1 cut(s) 1116
BbvI GCAGC 2 cut(s) 40, 491
BccI CCATC 6 cut(s) 245, 261, 1101, 1826, 2029, 2123
BciT130I CCWGG 8 cut(s) 1254, 1263, 1453, 1829, 2042, 2088, 2106, 2267
BciVI GTATCC 3 cut(s) 1309, 1441, 1474
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 5 cut(s) 318, 499, 712, 966, 1409
BfaI CTAG 8 cut(s) 111, 591, 1058, 1217, 1350, 1562, 1677, 1902
BfmI CTRYAG 3 cut(s) 1046, 1758, 2178
BfuAI ACCTGC 3 cut(s) 302, 813, 2286
BfuI GTATCC 3 cut(s) 1309, 1441, 1474
BisI GCNGC 4 cut(s) 54, 247, 288, 480
BlpI GCTNAGC 2 cut(s) 298, 2224
BlsI GCNGC 4 cut(s) 55, 248, 289, 481
Bme1390I CCNGG 8 cut(s) 1254, 1263, 1453, 1829, 2042, 2088, 2106, 2267
Bme18I GGWCC 5 cut(s) 742, 1381, 1802, 2038, 2263
BmeT110I CYCGRG 1 cut(s) 2258
BmgT120I GGNCC 6 cut(s) 742, 1381, 1802, 2038, 2102, 2263
BmiI GGNNCC 4 cut(s) 245, 743, 744, 1383
BmrFI CCNGG 8 cut(s) 1254, 1263, 1453, 1829, 2042, 2088, 2106, 2267
BmrI ACTGGG 1 cut(s) 249
BmsI GCATC 6 cut(s) 145, 218, 274, 875, 1643, 2062
BmuI ACTGGG 1 cut(s) 249
BpiI GAAGAC 1 cut(s) 767
BpmI CTGGAG 1 cut(s) 2118
Bpu10I CCTNAGC 1 cut(s) 1116
Bpu1102I GCTNAGC 2 cut(s) 298, 2224
BpuEI CTTGAG 2 cut(s) 1168, 1678
BsaAI YACGTR 1 cut(s) 578
BsaI GGTCTC 2 cut(s) 499, 966
BsaJI CCNNGG 6 cut(s) 1027, 1200, 1805, 1859, 2156, 2259
BsaWI WCCGGW 2 cut(s) 490, 1658
BsaXI ACNNNNNCTCC 4 cut(s) 441, 471, 2068, 2098
Bsc4I CCNNNNNNNGG 6 cut(s) 490, 752, 1118, 1390, 1943, 2235
Bse1I ACTGG 5 cut(s) 244, 681, 823, 933, 1238
Bse3DI GCAATG 2 cut(s) 78, 687
BseAI TCCGGA 1 cut(s) 1658
BseBI CCWGG 8 cut(s) 1254, 1263, 1453, 1829, 2042, 2088, 2106, 2267
BseDI CCNNGG 6 cut(s) 1027, 1200, 1805, 1859, 2156, 2259
BseGI GGATG 5 cut(s) 101, 1654, 1655, 1837, 1933
BseLI CCNNNNNNNGG 6 cut(s) 490, 752, 1118, 1390, 1943, 2235
BseMI GCAATG 2 cut(s) 78, 687
BseMII CTCAG 6 cut(s) 312, 632, 1107, 1145, 1776, 1808
BseNI ACTGG 5 cut(s) 244, 681, 823, 933, 1238
BseRI GAGGAG 1 cut(s) 1406
BseSI GKGCMC 3 cut(s) 909, 928, 1987
BseXI GCAGC 2 cut(s) 40, 491
BseYI CCCAGC 1 cut(s) 92
BsgI GTGCAG 2 cut(s) 198, 1939
Bsh1236I CGCG 2 cut(s) 249, 1567
BshFI GGCC 4 cut(s) 278, 654, 1111, 2104
BsiHKAI GWGCWC 2 cut(s) 909, 1987
BsiHKCI CYCGRG 1 cut(s) 2258
BsiSI CCGG 2 cut(s) 491, 1659
BslFI GGGAC 3 cut(s) 755, 1205, 1367
BslI CCNNNNNNNGG 6 cut(s) 490, 752, 1118, 1390, 1943, 2235
BsmAI GTCTC 5 cut(s) 318, 499, 712, 966, 1409
BsmFI GGGAC 3 cut(s) 755, 1205, 1367
BsmI GAATGC 3 cut(s) 379, 892, 1425
BsnI GGCC 4 cut(s) 278, 654, 1111, 2104
Bso31I GGTCTC 2 cut(s) 499, 966
BsoBI CYCGRG 1 cut(s) 2258
Bsp1286I GDGCHC 6 cut(s) 802, 909, 928, 1587, 1793, 1987
Bsp13I TCCGGA 1 cut(s) 1658
Bsp1407I TGTACA 1 cut(s) 1471
Bsp1720I GCTNAGC 2 cut(s) 298, 2224
Bsp19I CCATGG 3 cut(s) 1805, 1859, 2156
BspACI CCGC 5 cut(s) 247, 288, 408, 902, 2236
BspANI GGCC 4 cut(s) 278, 654, 1111, 2104
BspCNI CTCAG 6 cut(s) 311, 631, 1108, 1144, 1777, 1807
BspEI TCCGGA 1 cut(s) 1658
BspFNI CGCG 2 cut(s) 249, 1567
BspLI GGNNCC 4 cut(s) 245, 743, 744, 1383
BspMAI CTGCAG 1 cut(s) 1050
BspMI ACCTGC 3 cut(s) 302, 813, 2286
BspPI GGATC 8 cut(s) 229, 477, 703, 847, 876, 1216, 1954, 2140
BspQI GCTCTTC 1 cut(s) 1398
BspTNI GGTCTC 2 cut(s) 499, 966
BsrDI GCAATG 2 cut(s) 78, 687
BsrGI TGTACA 1 cut(s) 1471
BsrI ACTGG 5 cut(s) 244, 681, 823, 933, 1238
BssECI CCNNGG 6 cut(s) 1027, 1200, 1805, 1859, 2156, 2259
BssSI CACGAG 1 cut(s) 1003
BssT1I CCWWGG 5 cut(s) 1027, 1200, 1805, 1859, 2156
Bst2BI CACGAG 1 cut(s) 1003
Bst2UI CCWGG 8 cut(s) 1254, 1263, 1453, 1829, 2042, 2088, 2106, 2267
Bst4CI ACNGT 7 cut(s) 199, 1420, 1444, 1762, 1954, 2175, 2233
Bst6I CTCTTC 3 cut(s) 125, 375, 1398
BstAPI GCANNNNNTGC 3 cut(s) 485, 828, 2081
BstAUI TGTACA 1 cut(s) 1471
BstBAI YACGTR 1 cut(s) 578
BstC8I GCNNGC 5 cut(s) 181, 343, 890, 1113, 2238
BstDEI CTNAG 9 cut(s) 298, 618, 773, 1116, 1131, 1785, 1794, 1979, 2224
BstDSI CCRYGG 3 cut(s) 1805, 1859, 2156
BstF5I GGATG 5 cut(s) 101, 1654, 1655, 1837, 1933
BstFNI CGCG 2 cut(s) 249, 1567
BstHHI GCGC 2 cut(s) 1125, 1567
BstMAI GTCTC 5 cut(s) 318, 499, 712, 966, 1409
BstMWI GCNNNNNNNGC 7 cut(s) 284, 485, 828, 1163, 1573, 1582, 2081
BstNI CCWGG 8 cut(s) 1254, 1263, 1453, 1829, 2042, 2088, 2106, 2267
BstNSI RCATGY 2 cut(s) 345, 1304
BstSCI CCNGG 8 cut(s) 1252, 1261, 1451, 1827, 2040, 2086, 2104, 2265
BstSFI CTRYAG 3 cut(s) 1046, 1758, 2178
BstSLI GKGCMC 3 cut(s) 909, 928, 1987
BstSNI TACGTA 1 cut(s) 578
BstUI CGCG 2 cut(s) 249, 1567
BstV1I GCAGC 2 cut(s) 40, 491
BstV2I GAAGAC 1 cut(s) 767
BstX2I RGATCY 7 cut(s) 221, 469, 839, 868, 1208, 1959, 2132
BstXI CCANNNNNNTGG 1 cut(s) 1151
BstYI RGATCY 7 cut(s) 221, 469, 839, 868, 1208, 1959, 2132
BsuI GTATCC 3 cut(s) 1309, 1441, 1474
BsuRI GGCC 4 cut(s) 278, 654, 1111, 2104
BtgI CCRYGG 3 cut(s) 1805, 1859, 2156
BtgZI GCGATG 1 cut(s) 264
BtsCI GGATG 5 cut(s) 101, 1654, 1655, 1837, 1933
BtsIMutI CAGTG 6 cut(s) 674, 1440, 1644, 1782, 1821, 2238
BveI ACCTGC 3 cut(s) 302, 813, 2286
Cac8I GCNNGC 5 cut(s) 181, 343, 890, 1113, 2238
CaiI CAGNNNCTG 2 cut(s) 218, 917
CfoI GCGC 2 cut(s) 1125, 1567
Cfr13I GGNCC 6 cut(s) 742, 1381, 1802, 2038, 2102, 2263
CseI GACGC 1 cut(s) 204
CsiI ACCWGGT 1 cut(s) 2265
Csp6I GTAC 7 cut(s) 4, 328, 360, 579, 936, 985, 1472
CviQI GTAC 7 cut(s) 4, 328, 360, 579, 936, 985, 1472
DdeI CTNAG 9 cut(s) 298, 618, 773, 1116, 1131, 1785, 1794, 1979, 2224
DraIII CACNNNGTG 1 cut(s) 82
EaeI YGGCCR 1 cut(s) 276
Eam1104I CTCTTC 3 cut(s) 125, 375, 1398
EarI CTCTTC 3 cut(s) 125, 375, 1398
Eco105I TACGTA 1 cut(s) 578
Eco130I CCWWGG 5 cut(s) 1027, 1200, 1805, 1859, 2156
Eco24I GRGCYC 3 cut(s) 802, 1587, 1793
Eco31I GGTCTC 2 cut(s) 499, 966
Eco47I GGWCC 5 cut(s) 742, 1381, 1802, 2038, 2263
Eco57I CTGAAG 3 cut(s) 1080, 1563, 1860
Eco88I CYCGRG 1 cut(s) 2258
EcoO109I RGGNCCY 3 cut(s) 742, 1381, 2263
EcoRI GAATTC 1 cut(s) 2001
EcoRII CCWGG 8 cut(s) 1252, 1261, 1451, 1827, 2040, 2086, 2104, 2265
EcoT14I CCWWGG 5 cut(s) 1027, 1200, 1805, 1859, 2156
EcoT22I ATGCAT 2 cut(s) 347, 687
EcoT38I GRGCYC 3 cut(s) 802, 1587, 1793
ErhI CCWWGG 5 cut(s) 1027, 1200, 1805, 1859, 2156
FalI AAGNNNNNCTT 2 cut(s) 468, 500
FaqI GGGAC 3 cut(s) 755, 1205, 1367
FauI CCCGC 1 cut(s) 2229
FauNDI CATATG 3 cut(s) 142, 1073, 1476
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 4 cut(s) 54, 247, 288, 480
FokI GGATG 5 cut(s) 88, 1642, 1661, 1844, 1920
FriOI GRGCYC 3 cut(s) 802, 1587, 1793
Fsp4HI GCNGC 4 cut(s) 54, 247, 288, 480
FspBI CTAG 8 cut(s) 111, 591, 1058, 1217, 1350, 1562, 1677, 1902
GlaI GCGC 2 cut(s) 1124, 1566
GluI GCNGC 4 cut(s) 54, 247, 288, 480
GsaI CCCAGC 1 cut(s) 96
GsuI CTGGAG 1 cut(s) 2118
HaeIII GGCC 4 cut(s) 278, 654, 1111, 2104
HapII CCGG 2 cut(s) 491, 1659
HgaI GACGC 1 cut(s) 204
HhaI GCGC 2 cut(s) 1125, 1567
Hin6I GCGC 2 cut(s) 1123, 1565
HinP1I GCGC 2 cut(s) 1123, 1565
HincII GTYRAC 1 cut(s) 2017
HindII GTYRAC 1 cut(s) 2017
HindIII AAGCTT 1 cut(s) 2165
HinfI GANTC 9 cut(s) 311, 509, 662, 812, 848, 914, 1012, 1091, 1727
HpaII CCGG 2 cut(s) 491, 1659
HphI GGTGA 3 cut(s) 221, 518, 1342
Hpy166II GTNNAC 7 cut(s) 443, 575, 907, 1440, 1642, 1985, 2017
Hpy188I TCNGA 5 cut(s) 226, 385, 847, 1134, 1959
Hpy8I GTNNAC 7 cut(s) 443, 575, 907, 1440, 1642, 1985, 2017
HpyAV CCTTC 8 cut(s) 199, 316, 718, 1199, 1290, 1782, 1843, 1937
HpyCH4III ACNGT 7 cut(s) 199, 1420, 1444, 1762, 1954, 2175, 2233
HpyCH4IV ACGT 2 cut(s) 577, 944
HpyF10VI GCNNNNNNNGC 7 cut(s) 284, 485, 828, 1163, 1573, 1582, 2081
HpyF3I CTNAG 9 cut(s) 298, 618, 773, 1116, 1131, 1785, 1794, 1979, 2224
HpySE526I ACGT 2 cut(s) 577, 944
HspAI GCGC 2 cut(s) 1123, 1565
KflI GGGWCCC 1 cut(s) 742
Kpn2I TCCGGA 1 cut(s) 1658
Ksp22I TGATCA 1 cut(s) 226
LguI GCTCTTC 1 cut(s) 1398
LmnI GCTCC 4 cut(s) 243, 493, 1154, 2089
Lsp1109I GCAGC 2 cut(s) 40, 491
LweI GCATC 6 cut(s) 145, 218, 274, 875, 1643, 2062
MabI ACCWGGT 1 cut(s) 2265
MaeI CTAG 8 cut(s) 111, 591, 1058, 1217, 1350, 1562, 1677, 1902
MaeII ACGT 2 cut(s) 577, 944
MaeIII GTNAC 1 cut(s) 391
MflI RGATCY 7 cut(s) 221, 469, 839, 868, 1208, 1959, 2132
MhlI GDGCHC 6 cut(s) 802, 909, 928, 1587, 1793, 1987
MluCI AATT 8 cut(s) 106, 187, 1137, 1171, 1248, 1751, 2001, 2275
MlyI GAGTC 2 cut(s) 320, 1021
Mph1103I ATGCAT 2 cut(s) 347, 687
MroI TCCGGA 1 cut(s) 1658
MseI TTAA 8 cut(s) 20, 137, 1101, 1286, 1524, 1557, 1578, 2169
MslI CAYNNNNRTG 6 cut(s) 605, 630, 690, 897, 1864, 1938
MspI CCGG 2 cut(s) 491, 1659
MspR9I CCNGG 8 cut(s) 1254, 1263, 1453, 1829, 2042, 2088, 2106, 2267
Mva1269I GAATGC 3 cut(s) 379, 892, 1425
MvaI CCWGG 8 cut(s) 1254, 1263, 1453, 1829, 2042, 2088, 2106, 2267
MvnI CGCG 2 cut(s) 249, 1567
MwoI GCNNNNNNNGC 7 cut(s) 284, 485, 828, 1163, 1573, 1582, 2081
NcoI CCATGG 3 cut(s) 1805, 1859, 2156
NdeI CATATG 3 cut(s) 142, 1073, 1476
NlaIV GGNNCC 4 cut(s) 245, 743, 744, 1383
NmuCI GTSAC 1 cut(s) 391
NsiI ATGCAT 2 cut(s) 347, 687
NspI RCATGY 2 cut(s) 345, 1304
PaeI GCATGC 1 cut(s) 345
PaqCI CACCTGC 1 cut(s) 2286
PciSI GCTCTTC 1 cut(s) 1398
PcsI WCGNNNNNNNCGW 1 cut(s) 1996
PctI GAATGC 3 cut(s) 379, 892, 1425
PfeI GAWTC 7 cut(s) 509, 662, 812, 848, 914, 1091, 1727
PflFI GACNNNGTC 1 cut(s) 389
PflMI CCANNNNNTGG 1 cut(s) 752
PfoI TCCNGGA 3 cut(s) 1252, 1261, 2040
PkrI GCNGC 4 cut(s) 55, 248, 289, 481
PleI GAGTC 2 cut(s) 319, 1020
PpsI GAGTC 2 cut(s) 319, 1020
Ppu21I YACGTR 1 cut(s) 578
PpuMI RGGWCCY 3 cut(s) 742, 1381, 2263
PsiI TTATAA 1 cut(s) 1230
Psp1406I AACGTT 1 cut(s) 944
Psp5II RGGWCCY 3 cut(s) 742, 1381, 2263
Psp6I CCWGG 8 cut(s) 1252, 1261, 1451, 1827, 2040, 2086, 2104, 2265
PspFI CCCAGC 1 cut(s) 92
PspGI CCWGG 8 cut(s) 1252, 1261, 1451, 1827, 2040, 2086, 2104, 2265
PspN4I GGNNCC 4 cut(s) 245, 743, 744, 1383
PspPI GGNCC 6 cut(s) 742, 1381, 1802, 2038, 2102, 2263
PspPPI RGGWCCY 3 cut(s) 742, 1381, 2263
PstI CTGCAG 1 cut(s) 1050
PstNI CAGNNNCTG 2 cut(s) 218, 917
PsuI RGATCY 7 cut(s) 221, 469, 839, 868, 1208, 1959, 2132
PsyI GACNNNGTC 1 cut(s) 389
RsaI GTAC 7 cut(s) 5, 329, 361, 580, 937, 986, 1473
RsaNI GTAC 7 cut(s) 4, 328, 360, 579, 936, 985, 1472
RseI CAYNNNNRTG 6 cut(s) 605, 630, 690, 897, 1864, 1938
SapI GCTCTTC 1 cut(s) 1398
SaqAI TTAA 8 cut(s) 20, 137, 1101, 1286, 1524, 1557, 1578, 2169
SatI GCNGC 4 cut(s) 54, 247, 288, 480
Sau96I GGNCC 6 cut(s) 742, 1381, 1802, 2038, 2102, 2263
SchI GAGTC 2 cut(s) 320, 1021
ScrFI CCNGG 8 cut(s) 1254, 1263, 1453, 1829, 2042, 2088, 2106, 2267
SduI GDGCHC 6 cut(s) 802, 909, 928, 1587, 1793, 1987
SexAI ACCWGGT 1 cut(s) 2265
SfaNI GCATC 6 cut(s) 145, 218, 274, 875, 1643, 2062
SfcI CTRYAG 3 cut(s) 1046, 1758, 2178
SinI GGWCC 5 cut(s) 742, 1381, 1802, 2038, 2263
SmiMI CAYNNNNRTG 6 cut(s) 605, 630, 690, 897, 1864, 1938
SmlI CTYRAG 2 cut(s) 1183, 1693
SmoI CTYRAG 2 cut(s) 1183, 1693
SnaBI TACGTA 1 cut(s) 578
SphI GCATGC 1 cut(s) 345
Sse9I AATT 8 cut(s) 106, 187, 1137, 1171, 1248, 1751, 2001, 2275
SsiI CCGC 5 cut(s) 247, 288, 408, 902, 2236
SspI AATATT 3 cut(s) 427, 1284, 1528
SspMI CTAG 8 cut(s) 111, 591, 1058, 1217, 1350, 1562, 1677, 1902
StyD4I CCNGG 8 cut(s) 1252, 1261, 1451, 1827, 2040, 2086, 2104, 2265
StyI CCWWGG 5 cut(s) 1027, 1200, 1805, 1859, 2156
TaaI ACNGT 7 cut(s) 199, 1420, 1444, 1762, 1954, 2175, 2233
TaiI ACGT 2 cut(s) 580, 947
TaqI TCGA 4 cut(s) 31, 309, 474, 758
TaqII GACCGA 1 cut(s) 2130
TasI AATT 8 cut(s) 106, 187, 1137, 1171, 1248, 1751, 2001, 2275
TatI WGTACW 3 cut(s) 3, 984, 1471
TauI GCSGC 2 cut(s) 249, 290
TfiI GAWTC 7 cut(s) 509, 662, 812, 848, 914, 1091, 1727
Tru1I TTAA 8 cut(s) 20, 137, 1101, 1286, 1524, 1557, 1578, 2169
Tru9I TTAA 8 cut(s) 20, 137, 1101, 1286, 1524, 1557, 1578, 2169
TscAI CASTG 6 cut(s) 681, 1447, 1644, 1789, 1828, 2238
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 2 cut(s) 53, 479
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 4 cut(s) 451, 782, 1293, 1994
TspGWI ACGGA 1 cut(s) 1389
TspRI CASTG 6 cut(s) 681, 1447, 1644, 1789, 1828, 2238
Tth111I GACNNNGTC 1 cut(s) 389
Van91I CCANNNNNTGG 1 cut(s) 752
VneI GTGCAC 2 cut(s) 905, 1983
VpaK11BI GGWCC 5 cut(s) 742, 1381, 1802, 2038, 2263
XapI RAATTY 3 cut(s) 1171, 1751, 2001
XbaI TCTAGA 1 cut(s) 1057
XceI RCATGY 2 cut(s) 345, 1304
XcmI CCANNNNNNNNNTGG 1 cut(s) 1048
XspI CTAG 8 cut(s) 111, 591, 1058, 1217, 1350, 1562, 1677, 1902
Zsp2I ATGCAT 2 cut(s) 347, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.