RchiOBHm_Chr3g0452571

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007420
Physical Location & Seq
Forward (+)
3200107 .. 3209309
9203 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41971

Sequence Viewer

Length: 2292 bp
ATGCACCGAGTGGGTAGTGCTGGGAACACATCCAATTCTAGTCGCCCTCGTAAAGAGAAGAGATTAACATATGTATTGAATGATGCTGATGACAAAAAGCATTGTGCAGGCATAAATTGTTTGACTGTATTGAAGGCATCAGCGTCTGGATCTGATCACCTATTTACTGGGAGCCGCGATGGAACACTAAAAAGATGGGCTGTGGCCGAAGATGCCGCTACCTGCTCAGCAACCTTTGAGTCTCATGTGGATTGGGTTAATGATGCTGTTCTTGCAGGGGATAATACACTTGTTTCCTGTTCTTCAGATACCACCCTTAAGACGTGGGATTGCTTGTCAGATGGAACTTGTACCAGAACTCTTCGTCAGCATTCTGACTATGTCACTTGTCTTGCTGCCGCAGAAAAAAATAGCAATATTGTTGCCTCTGGTGGACTTGGAGGGGAGGTATTCATTTGGGATCTCGAAGCAGCCCTTGCTCCGGTCTCAAAGTCAGGTGATTCTGTGGAAGATGATTGTTCAAATGCCACCAATGGTTCTGGAAATGCACAACCGATTACAAGTTTACGTACTATCAGCTCTAGCAACAGCATTTCTATGCACACAACTCAGGCACATGGATATGTTCCAATCTCTGCCAAAGGCCATAAAGAATCAGTTTATGCACTGGCAATGCATGATAGTGGATCACTTCTTGTCTCTGGTGGAACAGAGAAGGTCGTGCGTGTTTGGGACCCAAGAAGTGGCTCGAAGACTATGAAACTAAGAGGGCATACAGATAATATCAGGGCTCTGCTTCTTGATTCTACTGGCAGGTTTTGCTTATCAGGATCTTCTGATTCAATGATTAGACTATGGGATCTTGGTCAGCAAAGATGCGTGCATTCTTATGCGGTGCACACAGATTCTGTTTGGGCACTTGCCAGTACGCCAACGTTTAGTCATGTATATAGTGGTGGGAGAGACCTTTCTTTGTACTTGACAGACTTGACCACAAGAGAGAGTCTTTTGCTTTGCCAAGGAGAACACCCCATTCTGCAGTTGGCTGTAGATGATGATAGCATATGGGTTGCAACAACAGATTCTTCAGTTAATAGATGGCCTGCTGAGGGGCGCAATCCTCAGAAAATTATCCAAAGAGGTGGTGCTTTCTTGGCTGGAAATTTGTCATTCTCAAGAGCAAGGGTGTCCTTAGAAGGATCTACCCTAGTCCCTGTTTATAAAGAACCAGTCTTTACAATTCCTGGAACTCCTGGAATAGTGCAACATGAAATATTAAATAATAGAAGGCATGTCTTGACAAAGGATACTGCTTCTTCGGTGAAGCTGTGGGAGATTACTAGAGGTGTTGTTATTGAGGATTACGGAAAGGTCTCGTTTGAGGAGAAAAAGGAAGAGCTTTTTGAGACTGTAAGCATTCCTGCATGGTTCACTGTGGATACCAGGCTTGGAAGTTTATCTGTACATATGGATACCCCACAATGCTTTTCAGCAGAGATGTATTCGGCAGACCTTAATATTGTAGGAAAGCCTGAAGATGACAAGGTTAATCTAGCGCGAGAAACGCTTAAAGGGCTCTTGGCTCATTGGTTGGGGAAAAGAAAGCAAAGATCGGGAACACAAGCATCAGTGAACGGGGATGTGTCATCCGGAAAGGAAATCACTCCTAGAAGTATTACCCACTCAAGAATGGAGGTGGATAGTAATGCCGAAAATGATTCAATGGTCTATCCTCCCTTTGAATTTTCTACAGTTTCCCCTCCTTCTATCATCACTGAGGGCTCTCAGGGAGGACCATGGAGAAAGAAGATCACTGACCTAGATGGAACTGAAGATGAGAAGGACTTTCCATGGTGGTGTCTGGATTGTGTGTTGAATAATCGCCTACCTCCTAGAGAAAATACCAAGTGCAGTTTTTATCTACATCCATGTGAAGGTTCAACTGTTCAGATCCTCACACAAGGAAAACTAAGTGCACCTCGTATATTACGAATTCATAAGGTTGTTAACTATGTAGTTGAAAAGATGGTCCTGGACAAACCATTAGATAGTGCAAATCCTGATGCAACATTTGCTCCTGGTCTTGCTGGAGGGCCAGGACAACTCTCTGCCATTGGAGATGGATCTTTTCGGTCTGGATTGAAGCCATGGCAGAAGCTTAAACCTTCTATAGAGATATTGTGCAACAATCAGGTCTTGTCGCCAGAAATGAGCTTAGCCACAGTGCGGGCTTATGTATGGAAGAAACCTGAGGACCTGGTTCTCAATTACAGGGTGGTTCAGGGCAGGTGA

Protein Analysis

763

Amino Acids

83.25

Weight (kDa)

6.33

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 26 - 157 8e-08 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 31 - 112 7.5e-06 TEP-1 second beta-propeller
WDR55 PF24796 32 - 154 1.2e-07 WDR55
WD40_Prp19 PF24814 36 - 158 7.4e-11 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 40 - 157 1.9e-17 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 40 - 157 7.4e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 42 - 155 5.8e-13 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 52 - 245 9.8e-19 THOC3 beta-propeller domain
WD40_Gbeta PF25391 119 - 340 6.9e-12 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 121 - 265 1.4e-12 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 123 - 363 2.2e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 149 - 325 2.8e-27 WDR3 first beta-propeller domain
WDR55 PF24796 188 - 292 9.1e-11 WDR55
WD40_WDHD1_1st PF24817 195 - 300 2.6e-12 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 210 - 287 9.8e-10 WDR3 second beta-propeller domain
EIF3I PF24805 210 - 301 1.2e-09 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 211 - 303 3e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 213 - 337 8.8e-08 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 213 - 297 4.6e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR41 PF25178 213 - 330 4.5e-06 WDR41
WD40 PF00400 214 - 245 5e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 214 - 369 6.1e-20 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 216 - 370 6.5e-20 THOC3 beta-propeller domain
WD40_Prp19 PF24814 217 - 343 5e-19 Prp19 WD40 domain
WD40 PF00400 251 - 287 4.8e-07 WD domain, G-beta repeat
DUF3337 PF11816 612 - 758 8.9e-29 Domain of unknown function (DUF3337)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1221
AarI CACCTGC 1 cut(s) 2277
Acc36I ACCTGC 3 cut(s) 230, 804, 2277
AccB7I CCANNNNNTGG 1 cut(s) 743
AccII CGCG 2 cut(s) 177, 1558
AccIII TCCGGA 1 cut(s) 1649
AciI CCGC 5 cut(s) 175, 216, 399, 893, 2227
AclI AACGTT 1 cut(s) 935
AclWI GGATC 8 cut(s) 157, 468, 694, 838, 867, 1207, 1945, 2131
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 3 cut(s) 1162, 1742, 1992
AcuI CTGAAG 4 cut(s) 288, 1071, 1554, 1851
AdeI CACNNNGTG 1 cut(s) 10
AfaI GTAC 5 cut(s) 352, 571, 928, 977, 1464
AfiI CCNNNNNNNGG 5 cut(s) 481, 743, 1109, 1934, 2226
AflII CTTAAG 1 cut(s) 317
AjiI CACGTC 1 cut(s) 324
AjnI CCWGG 7 cut(s) 1243, 1252, 1442, 2031, 2077, 2095, 2256
AluBI AGCT 5 cut(s) 579, 1327, 1399, 2158, 2214
AluI AGCT 5 cut(s) 579, 1327, 1399, 2158, 2214
Alw21I GWGCWC 2 cut(s) 900, 1978
Alw26I GTCTC 6 cut(s) 246, 490, 703, 957, 1378, 1400
Alw44I GTGCAC 2 cut(s) 896, 1974
AlwI GGATC 8 cut(s) 157, 468, 694, 838, 867, 1207, 1945, 2131
AlwNI CAGNNNCTG 2 cut(s) 146, 908
Aor13HI TCCGGA 1 cut(s) 1649
AoxI GGCC 4 cut(s) 204, 643, 1100, 2093
ApaLI GTGCAC 2 cut(s) 896, 1974
ApeKI GCWGC 2 cut(s) 395, 470
ApoI RAATTY 3 cut(s) 1162, 1742, 1992
AspLEI GCGC 2 cut(s) 1116, 1558
AspS9I GGNCC 5 cut(s) 733, 1793, 2029, 2093, 2254
AsuHPI GGTGA 3 cut(s) 149, 509, 1333
AvaII GGWCC 4 cut(s) 733, 1793, 2029, 2254
AxyI CCTNAGG 1 cut(s) 2250
BaeGI GKGCMC 3 cut(s) 900, 919, 1978
BanII GRGCYC 3 cut(s) 793, 1578, 1784
BbsI GAAGAC 1 cut(s) 758
Bbv12I GWGCWC 2 cut(s) 900, 1978
BbvCI CCTCAGC 1 cut(s) 1107
BbvI GCAGC 2 cut(s) 382, 482
BccI CCATC 7 cut(s) 173, 189, 335, 1092, 1817, 2020, 2114
BciT130I CCWGG 7 cut(s) 1245, 1254, 1444, 2033, 2079, 2097, 2258
BciVI GTATCC 3 cut(s) 1300, 1432, 1465
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 6 cut(s) 246, 490, 703, 957, 1378, 1400
BfaI CTAG 8 cut(s) 39, 582, 1208, 1341, 1553, 1668, 1820, 1893
BfmI CTRYAG 4 cut(s) 1037, 1047, 1749, 2169
BfrI CTTAAG 1 cut(s) 317
BfuAI ACCTGC 3 cut(s) 230, 804, 2277
BfuI GTATCC 3 cut(s) 1300, 1432, 1465
BisI GCNGC 5 cut(s) 175, 216, 396, 399, 471
BlpI GCTNAGC 2 cut(s) 226, 2215
BlsI GCNGC 5 cut(s) 176, 217, 397, 400, 472
Bme1390I CCNGG 7 cut(s) 1245, 1254, 1444, 2033, 2079, 2097, 2258
Bme18I GGWCC 4 cut(s) 733, 1793, 2029, 2254
BmgBI CACGTC 1 cut(s) 324
BmgT120I GGNCC 5 cut(s) 733, 1793, 2029, 2093, 2254
BmiI GGNNCC 3 cut(s) 173, 734, 735
BmrFI CCNGG 7 cut(s) 1245, 1254, 1444, 2033, 2079, 2097, 2258
BmrI ACTGGG 1 cut(s) 177
BmsI GCATC 7 cut(s) 73, 146, 202, 253, 866, 1634, 2053
BmuI ACTGGG 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 758
BpmI CTGGAG 1 cut(s) 2109
Bpu10I CCTNAGC 1 cut(s) 1107
Bpu1102I GCTNAGC 2 cut(s) 226, 2215
BpuEI CTTGAG 2 cut(s) 1159, 1669
BsaAI YACGTR 1 cut(s) 569
BsaI GGTCTC 3 cut(s) 490, 957, 1378
BsaJI CCNNGG 4 cut(s) 1018, 1796, 1850, 2147
BsaWI WCCGGW 2 cut(s) 481, 1649
BsaXI ACNNNNNCTCC 4 cut(s) 432, 462, 2059, 2089
Bsc4I CCNNNNNNNGG 5 cut(s) 481, 743, 1109, 1934, 2226
Bse1I ACTGG 5 cut(s) 172, 672, 814, 924, 1229
Bse21I CCTNAGG 1 cut(s) 2250
Bse3DI GCAATG 1 cut(s) 678
BseAI TCCGGA 1 cut(s) 1649
BseBI CCWGG 7 cut(s) 1245, 1254, 1444, 2033, 2079, 2097, 2258
BseDI CCNNGG 4 cut(s) 1018, 1796, 1850, 2147
BseGI GGATG 4 cut(s) 29, 1645, 1646, 1924
BseLI CCNNNNNNNGG 5 cut(s) 481, 743, 1109, 1934, 2226
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 7 cut(s) 240, 623, 1098, 1136, 1767, 1799, 2241
BseNI ACTGG 5 cut(s) 172, 672, 814, 924, 1229
BseRI GAGGAG 1 cut(s) 1397
BseSI GKGCMC 3 cut(s) 900, 919, 1978
BseXI GCAGC 2 cut(s) 382, 482
BseYI CCCAGC 1 cut(s) 20
BsgI GTGCAG 2 cut(s) 126, 1930
Bsh1236I CGCG 2 cut(s) 177, 1558
BshFI GGCC 4 cut(s) 206, 645, 1102, 2095
BsiHKAI GWGCWC 2 cut(s) 900, 1978
BsiSI CCGG 2 cut(s) 482, 1650
BslFI GGGAC 2 cut(s) 746, 1196
BslI CCNNNNNNNGG 5 cut(s) 481, 743, 1109, 1934, 2226
BsmAI GTCTC 6 cut(s) 246, 490, 703, 957, 1378, 1400
BsmFI GGGAC 2 cut(s) 746, 1196
BsmI GAATGC 3 cut(s) 370, 883, 1416
BsnI GGCC 4 cut(s) 206, 645, 1102, 2095
Bso31I GGTCTC 3 cut(s) 490, 957, 1378
Bsp1286I GDGCHC 6 cut(s) 793, 900, 919, 1578, 1784, 1978
Bsp13I TCCGGA 1 cut(s) 1649
Bsp1407I TGTACA 1 cut(s) 1462
Bsp1720I GCTNAGC 2 cut(s) 226, 2215
Bsp19I CCATGG 3 cut(s) 1796, 1850, 2147
BspACI CCGC 5 cut(s) 175, 216, 399, 893, 2227
BspANI GGCC 4 cut(s) 206, 645, 1102, 2095
BspCNI CTCAG 7 cut(s) 239, 622, 1099, 1135, 1768, 1798, 2242
BspEI TCCGGA 1 cut(s) 1649
BspFNI CGCG 2 cut(s) 177, 1558
BspLI GGNNCC 3 cut(s) 173, 734, 735
BspMAI CTGCAG 1 cut(s) 1041
BspMI ACCTGC 3 cut(s) 230, 804, 2277
BspPI GGATC 8 cut(s) 157, 468, 694, 838, 867, 1207, 1945, 2131
BspQI GCTCTTC 1 cut(s) 1389
BspTI CTTAAG 1 cut(s) 317
BspTNI GGTCTC 3 cut(s) 490, 957, 1378
BsrDI GCAATG 1 cut(s) 678
BsrGI TGTACA 1 cut(s) 1462
BsrI ACTGG 5 cut(s) 172, 672, 814, 924, 1229
BssECI CCNNGG 4 cut(s) 1018, 1796, 1850, 2147
BssT1I CCWWGG 4 cut(s) 1018, 1796, 1850, 2147
Bst2UI CCWGG 7 cut(s) 1245, 1254, 1444, 2033, 2079, 2097, 2258
Bst4CI ACNGT 6 cut(s) 127, 1411, 1435, 1753, 1945, 2224
Bst6I CTCTTC 3 cut(s) 53, 366, 1389
BstAFI CTTAAG 1 cut(s) 317
BstAPI GCANNNNNTGC 3 cut(s) 476, 819, 2072
BstAUI TGTACA 1 cut(s) 1462
BstBAI YACGTR 1 cut(s) 569
BstC8I GCNNGC 4 cut(s) 109, 881, 1104, 2229
BstDSI CCRYGG 3 cut(s) 1796, 1850, 2147
BstF5I GGATG 4 cut(s) 29, 1645, 1646, 1924
BstFNI CGCG 2 cut(s) 177, 1558
BstHHI GCGC 2 cut(s) 1116, 1558
BstMAI GTCTC 6 cut(s) 246, 490, 703, 957, 1378, 1400
BstMWI GCNNNNNNNGC 8 cut(s) 212, 272, 476, 819, 1154, 1564, 1573, 2072
BstNI CCWGG 7 cut(s) 1245, 1254, 1444, 2033, 2079, 2097, 2258
BstNSI RCATGY 1 cut(s) 1295
BstSCI CCNGG 7 cut(s) 1243, 1252, 1442, 2031, 2077, 2095, 2256
BstSFI CTRYAG 4 cut(s) 1037, 1047, 1749, 2169
BstSLI GKGCMC 3 cut(s) 900, 919, 1978
BstSNI TACGTA 1 cut(s) 569
BstUI CGCG 2 cut(s) 177, 1558
BstV1I GCAGC 2 cut(s) 382, 482
BstV2I GAAGAC 1 cut(s) 758
BstX2I RGATCY 7 cut(s) 149, 460, 830, 859, 1199, 1950, 2123
BstXI CCANNNNNNTGG 1 cut(s) 1142
BstYI RGATCY 7 cut(s) 149, 460, 830, 859, 1199, 1950, 2123
Bsu36I CCTNAGG 1 cut(s) 2250
BsuI GTATCC 3 cut(s) 1300, 1432, 1465
BsuRI GGCC 4 cut(s) 206, 645, 1102, 2095
BtgI CCRYGG 3 cut(s) 1796, 1850, 2147
BtgZI GCGATG 1 cut(s) 192
BtrI CACGTC 1 cut(s) 324
BtsCI GGATG 4 cut(s) 29, 1645, 1646, 1924
BtsIMutI CAGTG 6 cut(s) 665, 1431, 1635, 1773, 1812, 2229
BveI ACCTGC 3 cut(s) 230, 804, 2277
Cac8I GCNNGC 4 cut(s) 109, 881, 1104, 2229
CaiI CAGNNNCTG 2 cut(s) 146, 908
CfoI GCGC 2 cut(s) 1116, 1558
Cfr13I GGNCC 5 cut(s) 733, 1793, 2029, 2093, 2254
CseI GACGC 1 cut(s) 132
CsiI ACCWGGT 1 cut(s) 2256
Csp6I GTAC 5 cut(s) 351, 570, 927, 976, 1463
CviQI GTAC 5 cut(s) 351, 570, 927, 976, 1463
DraIII CACNNNGTG 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 3 cut(s) 53, 366, 1389
EarI CTCTTC 3 cut(s) 53, 366, 1389
Eco105I TACGTA 1 cut(s) 569
Eco130I CCWWGG 4 cut(s) 1018, 1796, 1850, 2147
Eco24I GRGCYC 3 cut(s) 793, 1578, 1784
Eco31I GGTCTC 3 cut(s) 490, 957, 1378
Eco47I GGWCC 4 cut(s) 733, 1793, 2029, 2254
Eco57I CTGAAG 4 cut(s) 288, 1071, 1554, 1851
Eco81I CCTNAGG 1 cut(s) 2250
EcoO109I RGGNCCY 2 cut(s) 733, 2254
EcoRI GAATTC 1 cut(s) 1992
EcoRII CCWGG 7 cut(s) 1243, 1252, 1442, 2031, 2077, 2095, 2256
EcoT14I CCWWGG 4 cut(s) 1018, 1796, 1850, 2147
EcoT22I ATGCAT 1 cut(s) 678
EcoT38I GRGCYC 3 cut(s) 793, 1578, 1784
ErhI CCWWGG 4 cut(s) 1018, 1796, 1850, 2147
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FaqI GGGAC 2 cut(s) 746, 1196
FauI CCCGC 1 cut(s) 2220
FauNDI CATATG 3 cut(s) 70, 1064, 1467
FbaI TGATCA 1 cut(s) 154
Fnu4HI GCNGC 5 cut(s) 175, 216, 396, 399, 471
FokI GGATG 4 cut(s) 16, 1633, 1652, 1911
FriOI GRGCYC 3 cut(s) 793, 1578, 1784
Fsp4HI GCNGC 5 cut(s) 175, 216, 396, 399, 471
FspBI CTAG 8 cut(s) 39, 582, 1208, 1341, 1553, 1668, 1820, 1893
GlaI GCGC 2 cut(s) 1115, 1557
GluI GCNGC 5 cut(s) 175, 216, 396, 399, 471
GsaI CCCAGC 1 cut(s) 24
GsuI CTGGAG 1 cut(s) 2109
HaeIII GGCC 4 cut(s) 206, 645, 1102, 2095
HapII CCGG 2 cut(s) 482, 1650
HgaI GACGC 1 cut(s) 132
HhaI GCGC 2 cut(s) 1116, 1558
Hin6I GCGC 2 cut(s) 1114, 1556
HinP1I GCGC 2 cut(s) 1114, 1556
HincII GTYRAC 1 cut(s) 2008
HindII GTYRAC 1 cut(s) 2008
HindIII AAGCTT 1 cut(s) 2156
HinfI GANTC 9 cut(s) 239, 500, 653, 803, 839, 905, 1003, 1082, 1718
HpaI GTTAAC 1 cut(s) 2008
HpaII CCGG 2 cut(s) 482, 1650
HphI GGTGA 3 cut(s) 149, 509, 1333
Hpy166II GTNNAC 7 cut(s) 434, 566, 898, 1431, 1633, 1976, 2008
Hpy188I TCNGA 7 cut(s) 154, 307, 340, 376, 838, 1125, 1950
Hpy8I GTNNAC 7 cut(s) 434, 566, 898, 1431, 1633, 1976, 2008
HpyAV CCTTC 8 cut(s) 127, 709, 1190, 1281, 1773, 1834, 1928, 2175
HpyCH4III ACNGT 6 cut(s) 127, 1411, 1435, 1753, 1945, 2224
HpyCH4IV ACGT 3 cut(s) 323, 568, 935
HpyF10VI GCNNNNNNNGC 8 cut(s) 212, 272, 476, 819, 1154, 1564, 1573, 2072
HpySE526I ACGT 3 cut(s) 323, 568, 935
HspAI GCGC 2 cut(s) 1114, 1556
KflI GGGWCCC 1 cut(s) 733
Kpn2I TCCGGA 1 cut(s) 1649
Ksp22I TGATCA 1 cut(s) 154
KspAI GTTAAC 1 cut(s) 2008
LguI GCTCTTC 1 cut(s) 1389
LmnI GCTCC 3 cut(s) 171, 484, 2080
Lsp1109I GCAGC 2 cut(s) 382, 482
LweI GCATC 7 cut(s) 73, 146, 202, 253, 866, 1634, 2053
MabI ACCWGGT 1 cut(s) 2256
MaeI CTAG 8 cut(s) 39, 582, 1208, 1341, 1553, 1668, 1820, 1893
MaeII ACGT 3 cut(s) 323, 568, 935
MaeIII GTNAC 1 cut(s) 382
MflI RGATCY 7 cut(s) 149, 460, 830, 859, 1199, 1950, 2123
MhlI GDGCHC 6 cut(s) 793, 900, 919, 1578, 1784, 1978
MluCI AATT 8 cut(s) 34, 115, 1128, 1162, 1239, 1742, 1992, 2266
MlyI GAGTC 2 cut(s) 248, 1012
Mph1103I ATGCAT 1 cut(s) 678
MroI TCCGGA 1 cut(s) 1649
MslI CAYNNNNRTG 6 cut(s) 596, 621, 681, 888, 1855, 1929
MspCI CTTAAG 1 cut(s) 317
MspI CCGG 2 cut(s) 482, 1650
MspR9I CCNGG 7 cut(s) 1245, 1254, 1444, 2033, 2079, 2097, 2258
Mva1269I GAATGC 3 cut(s) 370, 883, 1416
MvaI CCWGG 7 cut(s) 1245, 1254, 1444, 2033, 2079, 2097, 2258
MvnI CGCG 2 cut(s) 177, 1558
MwoI GCNNNNNNNGC 8 cut(s) 212, 272, 476, 819, 1154, 1564, 1573, 2072
NcoI CCATGG 3 cut(s) 1796, 1850, 2147
NdeI CATATG 3 cut(s) 70, 1064, 1467
NlaIV GGNNCC 3 cut(s) 173, 734, 735
NmuCI GTSAC 1 cut(s) 382
NsiI ATGCAT 1 cut(s) 678
NspI RCATGY 1 cut(s) 1295
PaqCI CACCTGC 1 cut(s) 2277
PciSI GCTCTTC 1 cut(s) 1389
PcsI WCGNNNNNNNCGW 1 cut(s) 1987
PctI GAATGC 3 cut(s) 370, 883, 1416
PfeI GAWTC 7 cut(s) 500, 653, 803, 839, 905, 1082, 1718
PflFI GACNNNGTC 1 cut(s) 380
PflMI CCANNNNNTGG 1 cut(s) 743
PfoI TCCNGGA 3 cut(s) 1243, 1252, 2031
PkrI GCNGC 5 cut(s) 176, 217, 397, 400, 472
PleI GAGTC 2 cut(s) 247, 1011
PpsI GAGTC 2 cut(s) 247, 1011
Ppu21I YACGTR 1 cut(s) 569
PpuMI RGGWCCY 2 cut(s) 733, 2254
PsiI TTATAA 1 cut(s) 1221
Psp1406I AACGTT 1 cut(s) 935
Psp5II RGGWCCY 2 cut(s) 733, 2254
Psp6I CCWGG 7 cut(s) 1243, 1252, 1442, 2031, 2077, 2095, 2256
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 7 cut(s) 1243, 1252, 1442, 2031, 2077, 2095, 2256
PspN4I GGNNCC 3 cut(s) 173, 734, 735
PspPI GGNCC 5 cut(s) 733, 1793, 2029, 2093, 2254
PspPPI RGGWCCY 2 cut(s) 733, 2254
PstI CTGCAG 1 cut(s) 1041
PstNI CAGNNNCTG 2 cut(s) 146, 908
PsuI RGATCY 7 cut(s) 149, 460, 830, 859, 1199, 1950, 2123
PsyI GACNNNGTC 1 cut(s) 380
RsaI GTAC 5 cut(s) 352, 571, 928, 977, 1464
RsaNI GTAC 5 cut(s) 351, 570, 927, 976, 1463
RseI CAYNNNNRTG 6 cut(s) 596, 621, 681, 888, 1855, 1929
SapI GCTCTTC 1 cut(s) 1389
SatI GCNGC 5 cut(s) 175, 216, 396, 399, 471
Sau96I GGNCC 5 cut(s) 733, 1793, 2029, 2093, 2254
SchI GAGTC 2 cut(s) 248, 1012
ScrFI CCNGG 7 cut(s) 1245, 1254, 1444, 2033, 2079, 2097, 2258
SduI GDGCHC 6 cut(s) 793, 900, 919, 1578, 1784, 1978
SexAI ACCWGGT 1 cut(s) 2256
SfaNI GCATC 7 cut(s) 73, 146, 202, 253, 866, 1634, 2053
SfcI CTRYAG 4 cut(s) 1037, 1047, 1749, 2169
SinI GGWCC 4 cut(s) 733, 1793, 2029, 2254
SmiMI CAYNNNNRTG 6 cut(s) 596, 621, 681, 888, 1855, 1929
SmlI CTYRAG 3 cut(s) 317, 1174, 1684
SmoI CTYRAG 3 cut(s) 317, 1174, 1684
SnaBI TACGTA 1 cut(s) 569
Sse9I AATT 8 cut(s) 34, 115, 1128, 1162, 1239, 1742, 1992, 2266
SsiI CCGC 5 cut(s) 175, 216, 399, 893, 2227
SspI AATATT 3 cut(s) 418, 1275, 1519
SspMI CTAG 8 cut(s) 39, 582, 1208, 1341, 1553, 1668, 1820, 1893
StyD4I CCNGG 7 cut(s) 1243, 1252, 1442, 2031, 2077, 2095, 2256
StyI CCWWGG 4 cut(s) 1018, 1796, 1850, 2147
TaaI ACNGT 6 cut(s) 127, 1411, 1435, 1753, 1945, 2224
TaiI ACGT 3 cut(s) 326, 571, 938
TaqI TCGA 2 cut(s) 465, 749
TaqII GACCGA 1 cut(s) 2121
TasI AATT 8 cut(s) 34, 115, 1128, 1162, 1239, 1742, 1992, 2266
TatI WGTACW 2 cut(s) 975, 1462
TauI GCSGC 3 cut(s) 177, 218, 401
TfiI GAWTC 7 cut(s) 500, 653, 803, 839, 905, 1082, 1718
TscAI CASTG 6 cut(s) 672, 1438, 1635, 1780, 1819, 2229
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 2 cut(s) 395, 470
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 4 cut(s) 442, 773, 1284, 1985
TspGWI ACGGA 1 cut(s) 1380
TspRI CASTG 6 cut(s) 672, 1438, 1635, 1780, 1819, 2229
Tth111I GACNNNGTC 1 cut(s) 380
Van91I CCANNNNNTGG 1 cut(s) 743
Vha464I CTTAAG 1 cut(s) 317
VneI GTGCAC 2 cut(s) 896, 1974
VpaK11BI GGWCC 4 cut(s) 733, 1793, 2029, 2254
XapI RAATTY 3 cut(s) 1162, 1742, 1992
XceI RCATGY 1 cut(s) 1295
XcmI CCANNNNNNNNNTGG 1 cut(s) 1039
XspI CTAG 8 cut(s) 39, 582, 1208, 1341, 1553, 1668, 1820, 1893
Zsp2I ATGCAT 1 cut(s) 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.