FvH4_6g04050

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016866
Physical Location & Seq
Forward (+)
2202301 .. 2205361
3061 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04050.t1

Sequence Viewer

Length: 2442 bp
ATGCGTTCTCGCTTCAAGCTCATCTTCTCATCTTCCTACTCGGCTTATGCTTATGCTTCTGCTTCTTCTTCTTCAATCAAAGTTACCAACTTTAACCAATCACAAGCTAAAGTTCACAACTTTTTGTCACTCTTACAAAGATTTTCGAAAACTCTCATCTGGGTCAACTCCATTCATGCCCAGATCATCACCAATGGGTTATTCAGAGACCAAAGTTTGGTCAGAAATCTCGTTGGGGTGTACTGTGATCTGGGTAGTTTGGTTTATGCACGCCAGGTGTTCGATGAATGTCCTCACCCAAAAACCATTCTTTACAATTCTATGATTTATGGGTATTTGAGAAAAGAGAGGTACAATGAGGCCCATGAGCTGTTTAGATTGATGGGTTTTCGTAATGTGGAGGTGGATAGTTATACATGTAACTATGCGCTCAAGGCATGTATGAGGCTTTCGGATTATGAAACGGGTGTGGAGGTTATTAAGAGAGCTGTGGATAGAGGAGTTGAGAGCAATGGGTTCTTTGGGAGTTCAATGATCAACTTCTTGATGAAATTTGGTAATGTAGATGAGGCGCGGAGGTGTTTTGATAGGATGGGGGAAAGAGATGTTGTTTGTTGGAATTCGATGATTGGTGGCTATGTGCAGGCTGCTCAGTTCAGCAAGGCATTTGATTTGTTTCATGAAATGTGGAGTTGCGGCATTAGGCCAAGTGCAGTAAGTATGCTGAGTATGATTCGAGTGTGCATTGATACTGCGGATTTCAAGCTTGCAAAATGTGTTCATGGTAGCATAGTTGGACTGGGAATGAGCAATGAGGTTTTGGTGCTTACCTCGTTGGTTGATATGTATAGTAATATTGGTGACATGCGTAGTGCATGTTGGGTTTTTGAGACCATGCCGAAAAGAAATTTGGTTTCATGGAATGTCATGGTCTCGGGATGTGTTCAAAATGGTATGGTGCATGAATCGTTTGCCTTCTTTGATAGATTAGTTGCAAGTGGTGTTAGGTTTGATTCAGGGACTATGGTGAGCCTAATTCAGGGTTGTTCTCAAATAGCTGCTTTGAATGGTGGAAAAATCCTACACGGTTGTTCATTAAGGAGGGGTTTTGAGTTAAACCCTATTTTATCCACTGCCATTGTTGATCTGTACTCTAAATGTGGTGCAGTAAAGAAAGCAACTTTTGTCTTTGACAGAATGAAACAGAGGAACGTTATTACCTGGACTGCTATGCTTGTGGGACTAACACAAAATGGGCATGCAGAAGAAGCCTTGAAGCTATTCTCTCAGATGCAAAAAGAGGGAGTTGCTGCCACTTCAGTGACACTGGTTAGTTTAGTTCATTCTTGTGCCCATTTGGGCTCTTTGAAGAAAGGAAAGGGTGTTCATGCTCACTTTATTCGGCAGGGCTATTCATTTGACGTAGTTAACATGACAGCCTTGATTGATATGTATGCCAAGTGTGGCACAATAGAGTATGCTGAGCGGATATTTAACAAGGGATCCATTTATAGAGATGTTATCCTATGGAACTCTATGATAACAAGCTATGGTATTCATGGCCATGGACAGCAAGCTCTTGATATGTATAAAAAAATGATGGAGGAGGGATTTGAGCCAAACGAAACCTCCGTTCTTTCTCTTCTAACTGCTTGTAGTCACTCGGGACTTCTTGAAGAAGGGATCGAGTTTTTCCACAGCATGGAGAGAAACCGTACTATTAGGCTGATTGAAAAACACTATGCTAGTTTTGTGGATCTCCTAAGCAGAGCAGGCAGATTTAAAGAAGCCGAGGCTTTGATTGAACAAATGCCTTTTAAACCAGGAAGTTCTGTTTTTGAAGCATTGCTGAGTGGATGTCAAGCTCACAAGAACATTGGACTGGGTATCAAGACGGCAGATAAATTACTTTGTATTGAATCCATGAATCCTAGTATTTATGTTGTGCTATCAAATATTTATGCTCAAGCAAGGAGATGGGATGATGTGAGCTACATTCGAAACCTGATGAGGACAAGAGGGCTAAGGAAAACACCCGGGTATAGCTTGATTGAAGTGGAAAATCAGGTCCACACATTCTTCGCTGGAGACAATTCGCATCCGCATTGGGCAGAGATTTATCAGCATTTAGAGAACATAAGAGCAGCAGTGGAGGCTTCTGGTTATATTCCTGATACAAGCAGTGTTCTTCGCAATGTCGACGAGCCAATGAAGATTAGGCTGCTATGGGGGCACAGCGAGAGACTAGCCATTGCATTTGGGCTCTTAAGTACACCAGCTGGGAGCTTGATTAGGATCACAAAGAATCTAAGAGTGTGTGTTGATTGTCACAATGTGACTAAACTTGTATCAAAGATGATGAAGAGGGAGCTTATTGTGAGAGATGCCAATCGTTTTCATCGCTTTGTGAATGGGAAGTGCTCTTGTAACGATTACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

814

Amino Acids

91.64

Weight (kDa)

8.93

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 100 - 148 3.5e-10 PPR repeat family
PPR_2 PF13041 201 - 248 4e-11 PPR repeat family
PPR PF01535 204 - 234 2.8e-07 PPR repeat
PPR_2 PF13041 303 - 350 6e-07 PPR repeat family
PPR PF01535 305 - 335 6.6e-06 PPR repeat
PPR_2 PF13041 403 - 452 7.7e-11 PPR repeat family
PPR PF01535 406 - 436 3.5e-07 PPR repeat
PPR_2 PF13041 505 - 553 1.5e-09 PPR repeat family
PPR PF01535 509 - 538 4.5e-06 PPR repeat
E_motif PF20431 623 - 685 1.4e-16 E motif
Eplus_motif PF20430 690 - 713 7.6e-08 E+ motif
DYW_deaminase PF14432 721 - 813 7.7e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016866)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04050
malus_domestica MD04G1216800.v1.1
prunus_persica Prupe.6G336500_v2.0.a1
pyrus_communis pycom04g19280
rosa_chinensis RchiOBHm_Chr3g0452611
rosa_laevigata RLG00000025578
rosa_multiflora Rmu_sc0011540.1_g000002 Rmu_sc0034042.1_g000001
rosa_roxburghii Rroxscaffold_6G00429650
rosa_rugosa Rorug02G0641700
rosa_samantha Rh3AG044200 Rh3BG046500 Rh3DG045500
rosa_wichuraiana Rw3G003440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 217, 1702
AccBSI CCGCTC 1 cut(s) 1486
AccI GTMKAC 1 cut(s) 2199
AccII CGCG 1 cut(s) 574
AciI CCGC 5 cut(s) 574, 696, 755, 1486, 2102
AclI AACGTT 1 cut(s) 1212
AclWI GGATC 5 cut(s) 1497, 1510, 1691, 1764, 2303
AcoI YGGCCR 1 cut(s) 1561
AcsI RAATTY 3 cut(s) 551, 619, 907
AcuI CTGAAG 1 cut(s) 1302
AdeI CACNNNGTG 1 cut(s) 2335
AfaI GTAC 5 cut(s) 242, 353, 1151, 1717, 2272
AfiI CCNNNNNNNGG 3 cut(s) 217, 1039, 1702
AflII CTTAAG 1 cut(s) 2266
AflIII ACRYGT 1 cut(s) 416
AjnI CCWGG 3 cut(s) 273, 1220, 1822
AjuI GAANNNNNNNTTGG 4 cut(s) 185, 217, 893, 925
AleI CACNNNNGTG 1 cut(s) 1319
AloI GAACNNNNNNTCC 2 cut(s) 1368, 1400
Alw21I GWGCWC 1 cut(s) 2423
Alw26I GTCTC 5 cut(s) 201, 884, 937, 2082, 2236
AlwI GGATC 5 cut(s) 1497, 1510, 1691, 1764, 2303
Ama87I CYCGRG 3 cut(s) 934, 1663, 2036
AoxI GGCC 3 cut(s) 360, 704, 1561
ApeKI GCWGC 5 cut(s) 647, 1058, 1310, 2144, 2221
ApoI RAATTY 3 cut(s) 551, 619, 907
Asp700I GAANNNNTTC 1 cut(s) 1280
AspLEI GCGC 2 cut(s) 430, 574
AspS9I GGNCC 2 cut(s) 361, 2068
AsuC2I CCSGG 2 cut(s) 2037, 2038
AsuHPI GGTGA 4 cut(s) 181, 287, 872, 1039
AsuII TTCGAA 2 cut(s) 146, 1999
AvaI CYCGRG 3 cut(s) 934, 1663, 2036
AvaII GGWCC 1 cut(s) 2068
BaeGI GKGCMC 2 cut(s) 1354, 2235
BalI TGGCCA 1 cut(s) 1563
BamHI GGATCC 1 cut(s) 1502
BanII GRGCYC 2 cut(s) 1364, 2265
Bbv12I GWGCWC 1 cut(s) 2423
BbvI GCAGC 5 cut(s) 634, 1045, 1297, 2156, 2208
BccI CCATC 4 cut(s) 376, 586, 1594, 1971
BceAI ACGGC 1 cut(s) 1911
BciT130I CCWGG 3 cut(s) 275, 1222, 1824
BclI TGATCA 1 cut(s) 534
BcnI CCSGG 2 cut(s) 2037, 2038
BcoDI GTCTC 5 cut(s) 201, 884, 937, 2082, 2236
BfaI CTAG 3 cut(s) 1746, 1932, 2246
BfrI CTTAAG 1 cut(s) 2266
BisI GCNGC 6 cut(s) 648, 697, 1059, 1311, 2145, 2222
BlpI GCTNAGC 1 cut(s) 1482
BlsI GCNGC 6 cut(s) 649, 698, 1060, 1312, 2146, 2223
Bme1390I CCNGG 5 cut(s) 275, 1222, 1824, 2037, 2038
Bme18I GGWCC 1 cut(s) 2068
BmeT110I CYCGRG 3 cut(s) 934, 1663, 2036
BmgT120I GGNCC 2 cut(s) 361, 2068
BmiI GGNNCC 1 cut(s) 1504
BmrFI CCNGG 5 cut(s) 275, 1222, 1824, 2037, 2038
BmrI ACTGGG 2 cut(s) 809, 1892
BmsI GCATC 3 cut(s) 1281, 2107, 2374
BmuI ACTGGG 2 cut(s) 809, 1892
BpmI CTGGAG 1 cut(s) 2106
Bpu10I CCTNAGC 2 cut(s) 1763, 2024
Bpu1102I GCTNAGC 1 cut(s) 1482
Bpu14I TTCGAA 2 cut(s) 146, 1999
BpuEI CTTGAG 2 cut(s) 416, 1950
BpuMI CCSGG 2 cut(s) 2037, 2038
BsaBI GATNNNNATC 2 cut(s) 2294, 2388
BsaI GGTCTC 3 cut(s) 201, 884, 937
BsaJI CCNNGG 3 cut(s) 1564, 1791, 2036
BsaXI ACNNNNNCTCC 2 cut(s) 1613, 1643
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 1039, 1702
Bse1I ACTGG 4 cut(s) 804, 1332, 1887, 2441
Bse3DI GCAATG 5 cut(s) 517, 817, 1844, 2200, 2250
Bse8I GATNNNNATC 2 cut(s) 2294, 2388
BseBI CCWGG 3 cut(s) 275, 1222, 1824
BseDI CCNNGG 3 cut(s) 1564, 1791, 2036
BseGI GGATG 5 cut(s) 597, 944, 1862, 1987, 2098
BseJI GATNNNNATC 2 cut(s) 2294, 2388
BseLI CCNNNNNNNGG 3 cut(s) 217, 1039, 1702
BseMI GCAATG 5 cut(s) 517, 817, 1844, 2200, 2250
BseMII CTCAG 5 cut(s) 665, 716, 1301, 1473, 1841
BseNI ACTGG 4 cut(s) 804, 1332, 1887, 2441
BseRI GAGGAG 2 cut(s) 513, 1619
BseSI GKGCMC 2 cut(s) 1354, 2235
BseXI GCAGC 5 cut(s) 634, 1045, 1297, 2156, 2208
BseYI CCCAGC 1 cut(s) 2279
BsgI GTGCAG 3 cut(s) 662, 732, 1185
Bsh1236I CGCG 1 cut(s) 574
BshFI GGCC 3 cut(s) 362, 706, 1563
BsiHKAI GWGCWC 1 cut(s) 2423
BsiHKCI CYCGRG 3 cut(s) 934, 1663, 2036
BsiSI CCGG 1 cut(s) 2037
BslFI GGGAC 3 cut(s) 1033, 1254, 1680
BslI CCNNNNNNNGG 3 cut(s) 217, 1039, 1702
BsmAI GTCTC 5 cut(s) 201, 884, 937, 2082, 2236
BsmFI GGGAC 3 cut(s) 1033, 1254, 1680
BsnI GGCC 3 cut(s) 362, 706, 1563
Bso31I GGTCTC 3 cut(s) 201, 884, 937
BsoBI CYCGRG 3 cut(s) 934, 1663, 2036
Bsp119I TTCGAA 2 cut(s) 146, 1999
Bsp1286I GDGCHC 5 cut(s) 1354, 1364, 2235, 2265, 2423
Bsp143I GATC 8 cut(s) 183, 247, 534, 1144, 1502, 1683, 1756, 2295
Bsp1720I GCTNAGC 1 cut(s) 1482
Bsp19I CCATGG 1 cut(s) 1564
BspACI CCGC 5 cut(s) 574, 696, 755, 1486, 2102
BspANI GGCC 3 cut(s) 362, 706, 1563
BspCNI CTCAG 5 cut(s) 664, 717, 1300, 1474, 1842
BspFNI CGCG 1 cut(s) 574
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 1 cut(s) 1504
BspPI GGATC 5 cut(s) 1497, 1510, 1691, 1764, 2303
BspT104I TTCGAA 2 cut(s) 146, 1999
BspTI CTTAAG 1 cut(s) 2266
BspTNI GGTCTC 3 cut(s) 201, 884, 937
BsrBI CCGCTC 1 cut(s) 1486
BsrDI GCAATG 5 cut(s) 517, 817, 1844, 2200, 2250
BsrI ACTGG 4 cut(s) 804, 1332, 1887, 2441
BssECI CCNNGG 3 cut(s) 1564, 1791, 2036
BssMI GATC 8 cut(s) 183, 247, 534, 1144, 1502, 1683, 1756, 2295
BssT1I CCWWGG 1 cut(s) 1564
Bst2UI CCWGG 3 cut(s) 275, 1222, 1824
Bst4CI ACNGT 3 cut(s) 245, 1088, 1715
Bst6I CTCTTC 2 cut(s) 1647, 2357
BstAFI CTTAAG 1 cut(s) 2266
BstBI TTCGAA 2 cut(s) 146, 1999
BstC8I GCNNGC 6 cut(s) 271, 645, 768, 1260, 1575, 1774
BstDEI CTNAG 8 cut(s) 651, 725, 1287, 1482, 1763, 1850, 2024, 2309
BstDSI CCRYGG 1 cut(s) 1564
BstENI CCTNNNNNAGG 1 cut(s) 1037
BstF5I GGATG 5 cut(s) 597, 944, 1862, 1987, 2098
BstFNI CGCG 1 cut(s) 574
BstHHI GCGC 2 cut(s) 430, 574
BstKTI GATC 8 cut(s) 186, 250, 537, 1147, 1505, 1686, 1759, 2298
BstMAI GTCTC 5 cut(s) 201, 884, 937, 2082, 2236
BstMBI GATC 8 cut(s) 183, 247, 534, 1144, 1502, 1683, 1756, 2295
BstMWI GCNNNNNNNGC 5 cut(s) 434, 1268, 1773, 2153, 2230
BstNI CCWGG 3 cut(s) 275, 1222, 1824
BstNSI RCATGY 5 cut(s) 420, 441, 868, 879, 1262
BstSCI CCNGG 5 cut(s) 273, 1220, 1822, 2035, 2036
BstSLI GKGCMC 2 cut(s) 1354, 2235
BstUI CGCG 1 cut(s) 574
BstV1I GCAGC 5 cut(s) 634, 1045, 1297, 2156, 2208
BstX2I RGATCY 2 cut(s) 1502, 1756
BstYI RGATCY 2 cut(s) 1502, 1756
BsuRI GGCC 3 cut(s) 362, 706, 1563
BtgI CCRYGG 1 cut(s) 1564
BtgZI GCGATG 1 cut(s) 2384
BtsCI GGATG 5 cut(s) 597, 944, 1862, 1987, 2098
BtsI GCAGTG 3 cut(s) 1131, 2154, 2188
BtsIMutI CAGTG 5 cut(s) 1131, 1325, 1326, 2154, 2188
Cac8I GCNNGC 6 cut(s) 271, 645, 768, 1260, 1575, 1774
CciI TCATGA 1 cut(s) 679
CfoI GCGC 2 cut(s) 430, 574
Cfr13I GGNCC 2 cut(s) 361, 2068
Cfr9I CCCGGG 1 cut(s) 2036
Csp6I GTAC 5 cut(s) 241, 352, 1150, 1716, 2271
CspCI CAANNNNNGTGG 2 cut(s) 1120, 1155
CviQI GTAC 5 cut(s) 241, 352, 1150, 1716, 2271
DdeI CTNAG 8 cut(s) 651, 725, 1287, 1482, 1763, 1850, 2024, 2309
DpnI GATC 8 cut(s) 185, 249, 536, 1146, 1504, 1685, 1758, 2297
DpnII GATC 8 cut(s) 183, 247, 534, 1144, 1502, 1683, 1756, 2295
DraI TTTAAA 2 cut(s) 1783, 1819
DraIII CACNNNGTG 1 cut(s) 2335
EaeI YGGCCR 1 cut(s) 1561
Eam1104I CTCTTC 2 cut(s) 1647, 2357
EarI CTCTTC 2 cut(s) 1647, 2357
Eco130I CCWWGG 1 cut(s) 1564
Eco24I GRGCYC 2 cut(s) 1364, 2265
Eco31I GGTCTC 3 cut(s) 201, 884, 937
Eco47I GGWCC 1 cut(s) 2068
Eco57I CTGAAG 1 cut(s) 1302
Eco88I CYCGRG 3 cut(s) 934, 1663, 2036
EcoNI CCTNNNNNAGG 1 cut(s) 1037
EcoRI GAATTC 1 cut(s) 619
EcoRII CCWGG 3 cut(s) 273, 1220, 1822
EcoT14I CCWWGG 1 cut(s) 1564
EcoT38I GRGCYC 2 cut(s) 1364, 2265
ErhI CCWWGG 1 cut(s) 1564
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FaqI GGGAC 3 cut(s) 1033, 1254, 1680
FbaI TGATCA 1 cut(s) 534
FblI GTMKAC 1 cut(s) 2199
Fnu4HI GCNGC 6 cut(s) 648, 697, 1059, 1311, 2145, 2222
FokI GGATG 5 cut(s) 604, 951, 1869, 1994, 2085
FriOI GRGCYC 2 cut(s) 1364, 2265
Fsp4HI GCNGC 6 cut(s) 648, 697, 1059, 1311, 2145, 2222
FspBI CTAG 3 cut(s) 1746, 1932, 2246
GlaI GCGC 2 cut(s) 429, 573
GluI GCNGC 6 cut(s) 648, 697, 1059, 1311, 2145, 2222
GsaI CCCAGC 1 cut(s) 2283
GsuI CTGGAG 1 cut(s) 2106
HaeIII GGCC 3 cut(s) 362, 706, 1563
HapII CCGG 1 cut(s) 2037
HhaI GCGC 2 cut(s) 430, 574
Hin6I GCGC 2 cut(s) 428, 572
HinP1I GCGC 2 cut(s) 428, 572
HincII GTYRAC 3 cut(s) 166, 1429, 2200
HindII GTYRAC 3 cut(s) 166, 1429, 2200
HindIII AAGCTT 1 cut(s) 764
HinfI GANTC 6 cut(s) 733, 965, 1013, 1919, 1927, 2305
HpaI GTTAAC 1 cut(s) 1429
HpaII CCGG 1 cut(s) 2037
HphI GGTGA 4 cut(s) 181, 287, 872, 1039
Hpy166II GTNNAC 7 cut(s) 115, 166, 241, 1429, 2071, 2200, 2273
Hpy188I TCNGA 4 cut(s) 206, 224, 454, 1290
Hpy188III TCNNGA 8 cut(s) 544, 680, 936, 1580, 1665, 1673, 1891, 2171
Hpy8I GTNNAC 7 cut(s) 115, 166, 241, 1429, 2071, 2200, 2273
Hpy99I CGWCG 1 cut(s) 2204
HpyAV CCTTC 2 cut(s) 985, 1673
HpyCH4III ACNGT 3 cut(s) 245, 1088, 1715
HpyCH4IV ACGT 2 cut(s) 1212, 1422
HpyF10VI GCNNNNNNNGC 5 cut(s) 434, 1268, 1773, 2153, 2230
HpyF3I CTNAG 8 cut(s) 651, 725, 1287, 1482, 1763, 1850, 2024, 2309
HpySE526I ACGT 2 cut(s) 1212, 1422
HspAI GCGC 2 cut(s) 428, 572
Ksp22I TGATCA 1 cut(s) 534
KspAI GTTAAC 1 cut(s) 1429
Kzo9I GATC 8 cut(s) 183, 247, 534, 1144, 1502, 1683, 1756, 2295
LmnI GCTCC 2 cut(s) 2283, 2368
Lsp1109I GCAGC 5 cut(s) 634, 1045, 1297, 2156, 2208
LweI GCATC 3 cut(s) 1281, 2107, 2374
MaeI CTAG 3 cut(s) 1746, 1932, 2246
MaeII ACGT 2 cut(s) 1212, 1422
MaeIII GTNAC 9 cut(s) 82, 126, 419, 860, 1321, 1658, 2327, 2335, 2426
MalI GATC 8 cut(s) 185, 249, 536, 1146, 1504, 1685, 1758, 2297
MbiI CCGCTC 1 cut(s) 1486
MboI GATC 8 cut(s) 183, 247, 534, 1144, 1502, 1683, 1756, 2295
MflI RGATCY 2 cut(s) 1502, 1756
MhlI GDGCHC 5 cut(s) 1354, 1364, 2235, 2265, 2423
MlsI TGGCCA 1 cut(s) 1563
MluCI AATT 7 cut(s) 316, 551, 619, 907, 1035, 1904, 2092
MluNI TGGCCA 1 cut(s) 1563
MmeI TCCRAC 2 cut(s) 596, 775
Mox20I TGGCCA 1 cut(s) 1563
MroXI GAANNNNTTC 1 cut(s) 1280
MscI TGGCCA 1 cut(s) 1563
MseI TTAA 9 cut(s) 93, 480, 1097, 1115, 1428, 1494, 1782, 1818, 2267
MslI CAYNNNNRTG 3 cut(s) 1319, 1347, 1563
Msp20I TGGCCA 1 cut(s) 1563
MspA1I CMGCKG 1 cut(s) 2279
MspCI CTTAAG 1 cut(s) 2266
MspI CCGG 1 cut(s) 2037
MspR9I CCNGG 5 cut(s) 275, 1222, 1824, 2037, 2038
MvaI CCWGG 3 cut(s) 275, 1222, 1824
MvnI CGCG 1 cut(s) 574
MwoI GCNNNNNNNGC 5 cut(s) 434, 1268, 1773, 2153, 2230
NciI CCSGG 2 cut(s) 2037, 2038
NcoI CCATGG 1 cut(s) 1564
NdeII GATC 8 cut(s) 183, 247, 534, 1144, 1502, 1683, 1756, 2295
NlaIV GGNNCC 1 cut(s) 1504
NmeAIII GCCGAG 2 cut(s) 20, 1816
NmuCI GTSAC 6 cut(s) 126, 860, 1321, 1658, 2327, 2335
NspI RCATGY 5 cut(s) 420, 441, 868, 879, 1262
NspV TTCGAA 2 cut(s) 146, 1999
OliI CACNNNNGTG 1 cut(s) 1319
PaeI GCATGC 1 cut(s) 1262
PagI TCATGA 1 cut(s) 679
PciI ACATGT 1 cut(s) 416
PcsI WCGNNNNNNNCGW 1 cut(s) 1629
PdmI GAANNNNTTC 1 cut(s) 1280
PfeI GAWTC 6 cut(s) 733, 965, 1013, 1919, 1927, 2305
PflMI CCANNNNNTGG 2 cut(s) 217, 1702
PkrI GCNGC 6 cut(s) 649, 698, 1060, 1312, 2146, 2223
PscI ACATGT 1 cut(s) 416
Psp1406I AACGTT 1 cut(s) 1212
Psp6I CCWGG 3 cut(s) 273, 1220, 1822
PspFI CCCAGC 1 cut(s) 2279
PspGI CCWGG 3 cut(s) 273, 1220, 1822
PspN4I GGNNCC 1 cut(s) 1504
PspPI GGNCC 2 cut(s) 361, 2068
PsuI RGATCY 2 cut(s) 1502, 1756
PvuII CAGCTG 1 cut(s) 2279
RsaI GTAC 5 cut(s) 242, 353, 1151, 1717, 2272
RsaNI GTAC 5 cut(s) 241, 352, 1150, 1716, 2271
RseI CAYNNNNRTG 3 cut(s) 1319, 1347, 1563
SalI GTCGAC 1 cut(s) 2198
SaqAI TTAA 9 cut(s) 93, 480, 1097, 1115, 1428, 1494, 1782, 1818, 2267
SatI GCNGC 6 cut(s) 648, 697, 1059, 1311, 2145, 2222
Sau3AI GATC 8 cut(s) 183, 247, 534, 1144, 1502, 1683, 1756, 2295
Sau96I GGNCC 2 cut(s) 361, 2068
ScrFI CCNGG 5 cut(s) 275, 1222, 1824, 2037, 2038
SduI GDGCHC 5 cut(s) 1354, 1364, 2235, 2265, 2423
SfaNI GCATC 3 cut(s) 1281, 2107, 2374
SfuI TTCGAA 2 cut(s) 146, 1999
SinI GGWCC 1 cut(s) 2068
SmaI CCCGGG 1 cut(s) 2038
SmiMI CAYNNNNRTG 3 cut(s) 1319, 1347, 1563
SmlI CTYRAG 3 cut(s) 431, 1965, 2266
SmoI CTYRAG 3 cut(s) 431, 1965, 2266
SphI GCATGC 1 cut(s) 1262
Sse9I AATT 7 cut(s) 316, 551, 619, 907, 1035, 1904, 2092
SsiI CCGC 5 cut(s) 574, 696, 755, 1486, 2102
SspI AATATT 2 cut(s) 856, 1957
SspMI CTAG 3 cut(s) 1746, 1932, 2246
StyD4I CCNGG 5 cut(s) 273, 1220, 1822, 2035, 2036
StyI CCWWGG 1 cut(s) 1564
TaaI ACNGT 3 cut(s) 245, 1088, 1715
TaiI ACGT 2 cut(s) 1215, 1425
TaqI TCGA 7 cut(s) 146, 282, 623, 736, 1686, 1999, 2199
TasI AATT 7 cut(s) 316, 551, 619, 907, 1035, 1904, 2092
TatI WGTACW 3 cut(s) 240, 1149, 2270
TauI GCSGC 1 cut(s) 699
TfiI GAWTC 6 cut(s) 733, 965, 1013, 1919, 1927, 2305
Tru1I TTAA 9 cut(s) 93, 480, 1097, 1115, 1428, 1494, 1782, 1818, 2267
Tru9I TTAA 9 cut(s) 93, 480, 1097, 1115, 1428, 1494, 1782, 1818, 2267
TscAI CASTG 5 cut(s) 1138, 1326, 1332, 2154, 2188
TseFI GTSAC 6 cut(s) 126, 860, 1321, 1658, 2327, 2335
TseI GCWGC 5 cut(s) 647, 1058, 1310, 2144, 2221
Tsp45I GTSAC 6 cut(s) 126, 860, 1321, 1658, 2327, 2335
TspGWI ACGGA 1 cut(s) 1621
TspMI CCCGGG 1 cut(s) 2036
TspRI CASTG 5 cut(s) 1138, 1326, 1332, 2154, 2188
Van91I CCANNNNNTGG 2 cut(s) 217, 1702
Vha464I CTTAAG 1 cut(s) 2266
VpaK11BI GGWCC 1 cut(s) 2068
XagI CCTNNNNNAGG 1 cut(s) 1037
XapI RAATTY 3 cut(s) 551, 619, 907
XceI RCATGY 5 cut(s) 420, 441, 868, 879, 1262
XmaI CCCGGG 1 cut(s) 2036
XmiI GTMKAC 1 cut(s) 2199
XmnI GAANNNNTTC 1 cut(s) 1280
XspI CTAG 3 cut(s) 1746, 1932, 2246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.