pycom04g19280

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0016866
Physical Location & Seq
Reverse (-)
21048909 .. 21051350
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g19280.1

Sequence Viewer

Length: 2442 bp
ATGCGTAATCTGTTCCTCCATATCCACGGATTATTTCCCAAAATACCCTTCAATCTCAGCCCCTCGTCTTCCTTCTCAGTCTACGCTTCTTGTTCTTATTCTTCTGCAAATGTTCACGAGTTCTCGTCACTCTTACAACAATTTGCACAAAAGCTTATCTGGGTCAAATCCATTCATGCCCAGATCATCATTAATTCTATATCTAATGACCAAATTCTGGGTACAAAACTCGTTAAAGTGTACTCGGATTTGGGAAGTTTGGATAATGCCCGCCAAGTGTTCGATCAATTTCCTCAACCAGAAACCATTCTATGCAATGCCATGGTTTATGGGTATTTGAGAAAAGAGAGGTACAGTGAGAGCCTTGAGTTGTTTAATTTGATGGGTTCTTATCATTTGGAGATTGATAGTTACACGTGTAATTATGCGCTGAAGGCATGCATGGGCTTATTGGATTACAGAAGGGGGATGGAAGTTATTAAGAGAGCTGTGGATAAAGGAATTGAAAGTGATCGATTTTTGGGGAGTTCGATGATTCATTTCTTGGTGAAGTTTGATAAGGTTGATTGTGCGCAAAGGTTTTTCGATAGGATGCCAGAAAGAGATGTTGTTTGTTGGAATTCCATGATTGGTGGCTATGTGCAATCTGGTCAGTTTAGTAAGGCAGTTGATATGTTTGTAGAAATGTGTAGTTGTGGGGTTAAGCCGAGTCTGGTTACTATGCTAAGCTTGATTCAAGTTTGCATTAAAAGTGGGAATTTCAAGCTTGCAAAATGTGTTCATGGATGCATAATTAGACTAGGAATGGGTAATGAGGTTTTACTACGTACCTCGCTAGTGGATATGTATAATAATATGGGTGACATGCGAAGTTCCTTTTCTGTTTTTGAGACCATGCCCATCAGAAGTTTGATTTCGTGGAATGTCATGATATCGGGGTGCATTCAAAATGGTCTGGTACACGAGTCTTTTGGTTTCTTCCACAGATTGGTCACAGATGGTGGTAGGTTTGATTCGGGAACCATGGTGAGTCTTGTTCAGGGATGTTCTCAAACAGCTGATTTGGAAACTGGGAAAATCCTACATTGTTGTGTTTTTAGAATGGGTTTTGATCCAAATCCTATTTTGTCTACTGCCATTGTTGATTTGTACTCTAAATGTGGTGCAATAAAGCTAGCAACATTTGTCTTTGATAGAATGAAAGAGAAGAACGTGATTACATGGACGGCCATGCTGGTAGGATTGACGCAAAATGGGCATGCAGAAGAAGCCTTGAAACTGTTTAGTCAGATGCAAGAAGAGGGAATTGCTGCTAATTTGGTAACTCTTGTTAGTTTAGTCCATTCCTGTTCACATTTGGGCTCTCTGAAGAAAGGCAGGAGTGTTCATGCTCATTTGATCCGGAATGGGCATGAAGTTGATGTAGTTTCCATGACAGCCTTGATTGATATGTATGCAAAGTGTGGAAAAATAAAGTATGCTGAGAGAACATTTGAAAATGGCGCCTTTTGTAGAGATGTTATCCTATGGAATTCTATGATAACTAGCTACGGTATTCACGGATATGGACTGCAAGCTCTTGGTATCTATAGAAGAATGAAGGAGGAGGGGTATAAGCCAAATGAAACCTCCCTTCTTTCTCTTCTAACTGCTTGTAGTCACTCCGGACTTGTGGAAGAGGGTATCAAACTATTCCACAGCATGGAGAGAGATCGTGGCATTAGGCTGACTGAAAAACACTACGCTAGTTTTGTGGATCTTTTATGCCGAGCAGGCAGACTTGAAGAAGCTGAGGCATTGATTAAGCAAATACCTTTTGAACCAGGAAGTTCGGTTCTTGAAGCATTGCTTAATGGATGTCAGGCTCACAAGAACATTGATATCGGCATAAAGACAGCTGACAGATTGCTTTGTATAGATTCCATGAATCCTTCAATTTATGTTGTGATATCAAACATTTATGCTCAAGCACGGAGATGGGATGCTGTAAATTATATCCGGAGCCTCATGAGGACAAGGGGACTCAGAAAAATACCGGGTTATAGCTTGATTGAAGTCGGAAACCAGGTTCATACATTTTTCGCCGGAGACAACTCCCATCCGAATTGGGCAGAGATATATCAGCATTTAGAGGATATAAGAGCAGCAGTGGAGGCTTCTGGTTATGTCCCGGATACAAGTTGTGTTCTTCGTGACGTGGATGAGCCGATGAAGGTCAGGTTGCTATGGGGACATAGTGAGAGATTAGCCATTGCATTCGGACTTTTAAACACGCCGGCAGGAAGCTTGATTAGGATCACCAAGAATCTGCGTGTGTGTGTTGACTGTCACAATGTGACTAAATATATATCCAAGATAGTGAGGAGGGAACTTATTGTGAGAGATGCCAATCGTTTCCATCACTTTGTCGATGGGAAATGTTCTTGCAATGATTACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

814

Amino Acids

91.5

Weight (kDa)

8.32

Isoelectric Point (pI)

30.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 201 - 249 2.9e-12 PPR repeat family
PPR PF01535 204 - 234 2.1e-08 PPR repeat
PPR_2 PF13041 403 - 451 2.8e-10 PPR repeat family
PPR PF01535 406 - 436 2.5e-07 PPR repeat
PPR_2 PF13041 505 - 553 2.3e-07 PPR repeat family
E_motif PF20431 623 - 685 2.7e-16 E motif
Eplus_motif PF20430 690 - 713 5.6e-08 E+ motif
DYW_deaminase PF14432 721 - 813 4.8e-43 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016866)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04050
malus_domestica MD04G1216800.v1.1
prunus_persica Prupe.6G336500_v2.0.a1
pyrus_communis pycom04g19280
rosa_chinensis RchiOBHm_Chr3g0452611
rosa_laevigata RLG00000025578
rosa_multiflora Rmu_sc0011540.1_g000002 Rmu_sc0034042.1_g000001
rosa_roxburghii Rroxscaffold_6G00429650
rosa_rugosa Rorug02G0641700
rosa_samantha Rh3AG044200 Rh3BG046500 Rh3DG045500
rosa_wichuraiana Rw3G003440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 573
AccB1I GGYRCC 1 cut(s) 1502
AccB7I CCANNNNNTGG 3 cut(s) 217, 988, 1702
AccI GTMKAC 2 cut(s) 81, 1130
AccIII TCCGGA 3 cut(s) 1401, 1664, 1998
AciI CCGC 1 cut(s) 271
AclWI GGATC 4 cut(s) 1106, 1393, 1764, 2303
AcoI YGGCCR 1 cut(s) 1227
AcsI RAATTY 4 cut(s) 213, 619, 757, 1531
AcuI CTGAAG 2 cut(s) 452, 1388
AcvI CACGTG 1 cut(s) 417
AcyI GRCGYC 1 cut(s) 1503
AdeI CACNNNGTG 1 cut(s) 2335
AfaI GTAC 6 cut(s) 223, 242, 353, 829, 960, 1151
AfiI CCNNNNNNNGG 3 cut(s) 217, 988, 1702
AflIII ACRYGT 2 cut(s) 414, 416
AjiI CACGTC 1 cut(s) 2197
AjnI CCWGG 2 cut(s) 1822, 2064
AjuI GAANNNNNNNTTGG 2 cut(s) 32, 64
AloI GAACNNNNNNTCC 4 cut(s) 1818, 1850, 2052, 2084
Alw26I GTCTC 2 cut(s) 884, 2082
AlwI GGATC 4 cut(s) 1106, 1393, 1764, 2303
Aor13HI TCCGGA 3 cut(s) 1401, 1664, 1998
AoxI GGCC 1 cut(s) 1227
ApeKI GCWGC 2 cut(s) 1310, 2144
ApoI RAATTY 4 cut(s) 213, 619, 757, 1531
AseI ATTAAT 1 cut(s) 192
Asp700I GAANNNNTTC 1 cut(s) 306
AspLEI GCGC 3 cut(s) 430, 574, 1505
AsuC2I CCSGG 2 cut(s) 2037, 2171
AsuHPI GGTGA 4 cut(s) 559, 872, 1039, 2290
AsuNHI GCTAGC 1 cut(s) 1174
BaeI ACNNNNGTAYC 2 cut(s) 950, 983
BanI GGYRCC 1 cut(s) 1502
BanII GRGCYC 1 cut(s) 1364
BauI CACGAG 2 cut(s) 116, 962
BbrPI CACGTG 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 60
BbvCI CCTCAGC 1 cut(s) 1791
BbvI GCAGC 2 cut(s) 1297, 2156
BccI CCATC 8 cut(s) 376, 463, 908, 992, 1971, 2106, 2405, 2406
BceAI ACGGC 1 cut(s) 1242
BciT130I CCWGG 2 cut(s) 1824, 2066
BciVI GTATCC 1 cut(s) 2167
BcnI CCSGG 2 cut(s) 2037, 2171
BcoDI GTCTC 2 cut(s) 884, 2082
BfaI CTAG 5 cut(s) 800, 836, 1175, 1545, 1746
BfmI CTRYAG 1 cut(s) 1588
BfoI RGCGCY 1 cut(s) 1506
BfuI GTATCC 1 cut(s) 2167
BglI GCCNNNNNGGC 1 cut(s) 1773
BisI GCNGC 2 cut(s) 1311, 2145
BlpI GCTNAGC 1 cut(s) 725
BlsI GCNGC 2 cut(s) 1312, 2146
Bme1390I CCNGG 4 cut(s) 1824, 2037, 2066, 2171
BmgBI CACGTC 1 cut(s) 2197
BmiI GGNNCC 3 cut(s) 1021, 1504, 2003
BmrFI CCNGG 4 cut(s) 1824, 2037, 2066, 2171
BmrI ACTGGG 1 cut(s) 1080
BmsI GCATC 5 cut(s) 582, 776, 1281, 1972, 2374
BmtI GCTAGC 1 cut(s) 1178
BmuI ACTGGG 1 cut(s) 1080
BpiI GAAGAC 1 cut(s) 60
Bpu10I CCTNAGC 1 cut(s) 1791
Bpu1102I GCTNAGC 1 cut(s) 725
BpuEI CTTGAG 2 cut(s) 386, 1950
BpuMI CCSGG 2 cut(s) 2037, 2171
Bsa29I ATCGAT 1 cut(s) 514
BsaAI YACGTR 2 cut(s) 417, 827
BsaBI GATNNNNATC 3 cut(s) 1116, 2294, 2388
BsaHI GRCGYC 1 cut(s) 1503
BsaI GGTCTC 1 cut(s) 884
BsaJI CCNNGG 3 cut(s) 25, 321, 1023
BsaWI WCCGGW 3 cut(s) 1401, 1664, 1998
BsaXI ACNNNNNCTCC 2 cut(s) 1595, 1625
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 988, 1702
Bse118I RCCGGY 1 cut(s) 2275
Bse1I ACTGG 2 cut(s) 1075, 2441
Bse3DI GCAATG 4 cut(s) 322, 1844, 2250, 2434
Bse8I GATNNNNATC 3 cut(s) 1116, 2294, 2388
BseAI TCCGGA 3 cut(s) 1401, 1664, 1998
BseBI CCWGG 2 cut(s) 1824, 2066
BseCI ATCGAT 1 cut(s) 514
BseDI CCNNGG 3 cut(s) 25, 321, 1023
BseGI GGATG 8 cut(s) 474, 597, 791, 1049, 1862, 1987, 2098, 2206
BseJI GATNNNNATC 3 cut(s) 1116, 2294, 2388
BseLI CCNNNNNNNGG 3 cut(s) 217, 988, 1702
BseMI GCAATG 4 cut(s) 322, 1844, 2250, 2434
BseMII CTCAG 5 cut(s) 70, 90, 1473, 1782, 2038
BseNI ACTGG 2 cut(s) 1075, 2441
BseRI GAGGAG 2 cut(s) 1619, 2377
BseXI GCAGC 2 cut(s) 1297, 2156
BshFI GGCC 1 cut(s) 1229
BshNI GGYRCC 1 cut(s) 1502
BshVI ATCGAT 1 cut(s) 514
BsiSI CCGG 7 cut(s) 1402, 1665, 1999, 2036, 2085, 2171, 2276
BslFI GGGAC 3 cut(s) 2034, 2153, 2244
BslI CCNNNNNNNGG 3 cut(s) 217, 988, 1702
BsmAI GTCTC 2 cut(s) 884, 2082
BsmFI GGGAC 3 cut(s) 2034, 2153, 2244
BsmI GAATGC 2 cut(s) 942, 2255
BsnI GGCC 1 cut(s) 1229
Bso31I GGTCTC 1 cut(s) 884
Bsp1286I GDGCHC 1 cut(s) 1364
Bsp13I TCCGGA 3 cut(s) 1401, 1664, 1998
Bsp143I GATC 8 cut(s) 183, 283, 511, 1111, 1398, 1711, 1756, 2295
Bsp1720I GCTNAGC 1 cut(s) 725
Bsp19I CCATGG 2 cut(s) 321, 1023
BspACI CCGC 1 cut(s) 271
BspANI GGCC 1 cut(s) 1229
BspCNI CTCAG 5 cut(s) 69, 89, 1474, 1783, 2037
BspDI ATCGAT 1 cut(s) 514
BspEI TCCGGA 3 cut(s) 1401, 1664, 1998
BspHI TCATGA 2 cut(s) 927, 2007
BspLI GGNNCC 3 cut(s) 1021, 1504, 2003
BspOI GCTAGC 1 cut(s) 1178
BspPI GGATC 4 cut(s) 1106, 1393, 1764, 2303
BspT107I GGYRCC 1 cut(s) 1502
BspTNI GGTCTC 1 cut(s) 884
BsrDI GCAATG 4 cut(s) 322, 1844, 2250, 2434
BsrFI RCCGGY 1 cut(s) 2275
BsrI ACTGG 2 cut(s) 1075, 2441
BssAI RCCGGY 1 cut(s) 2275
BssECI CCNNGG 3 cut(s) 25, 321, 1023
BssMI GATC 8 cut(s) 183, 283, 511, 1111, 1398, 1711, 1756, 2295
BssNI GRCGYC 1 cut(s) 1503
BssSI CACGAG 2 cut(s) 116, 962
BssT1I CCWWGG 2 cut(s) 321, 1023
Bst2BI CACGAG 2 cut(s) 116, 962
Bst2UI CCWGG 2 cut(s) 1824, 2066
Bst4CI ACNGT 4 cut(s) 356, 1281, 1553, 2327
Bst6I CTCTTC 3 cut(s) 1293, 1647, 1671
BstACI GRCGYC 1 cut(s) 1503
BstBAI YACGTR 2 cut(s) 417, 827
BstC8I GCNNGC 8 cut(s) 271, 439, 768, 1176, 1260, 1575, 1774, 2277
BstDEI CTNAG 6 cut(s) 56, 76, 725, 1482, 1791, 2024
BstDSI CCRYGG 3 cut(s) 25, 321, 1023
BstF5I GGATG 8 cut(s) 474, 597, 791, 1049, 1862, 1987, 2098, 2206
BstH2I RGCGCY 1 cut(s) 1506
BstHHI GCGC 3 cut(s) 430, 574, 1505
BstKTI GATC 8 cut(s) 186, 286, 514, 1114, 1401, 1714, 1759, 2298
BstMAI GTCTC 2 cut(s) 884, 2082
BstMBI GATC 8 cut(s) 183, 283, 511, 1111, 1398, 1711, 1756, 2295
BstMWI GCNNNNNNNGC 5 cut(s) 434, 1255, 1268, 1773, 2153
BstNI CCWGG 2 cut(s) 1824, 2066
BstNSI RCATGY 3 cut(s) 441, 868, 1262
BstSCI CCNGG 4 cut(s) 1822, 2035, 2064, 2169
BstSFI CTRYAG 1 cut(s) 1588
BstSNI TACGTA 1 cut(s) 827
BstV1I GCAGC 2 cut(s) 1297, 2156
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 1756
BstYI RGATCY 1 cut(s) 1756
Bsu15I ATCGAT 1 cut(s) 514
BsuI GTATCC 1 cut(s) 2167
BsuRI GGCC 1 cut(s) 1229
BsuTUI ATCGAT 1 cut(s) 514
BtgI CCRYGG 3 cut(s) 25, 321, 1023
BtrI CACGTC 1 cut(s) 2197
BtsCI GGATG 8 cut(s) 474, 597, 791, 1049, 1862, 1987, 2098, 2206
BtsI GCAGTG 1 cut(s) 2154
BtsIMutI CAGTG 2 cut(s) 361, 2154
Cac8I GCNNGC 8 cut(s) 271, 439, 768, 1176, 1260, 1575, 1774, 2277
CciI TCATGA 2 cut(s) 927, 2007
CfoI GCGC 3 cut(s) 430, 574, 1505
Cfr10I RCCGGY 1 cut(s) 2275
ClaI ATCGAT 1 cut(s) 514
CseI GACGC 1 cut(s) 1255
CsiI ACCWGGT 1 cut(s) 2064
Csp6I GTAC 6 cut(s) 222, 241, 352, 828, 959, 1150
CviQI GTAC 6 cut(s) 222, 241, 352, 828, 959, 1150
DdeI CTNAG 6 cut(s) 56, 76, 725, 1482, 1791, 2024
DinI GGCGCC 1 cut(s) 1504
DpnI GATC 8 cut(s) 185, 285, 513, 1113, 1400, 1713, 1758, 2297
DpnII GATC 8 cut(s) 183, 283, 511, 1111, 1398, 1711, 1756, 2295
DraI TTTAAA 1 cut(s) 2268
DraIII CACNNNGTG 1 cut(s) 2335
EaeI YGGCCR 1 cut(s) 1227
Eam1104I CTCTTC 3 cut(s) 1293, 1647, 1671
EarI CTCTTC 3 cut(s) 1293, 1647, 1671
Eco105I TACGTA 1 cut(s) 827
Eco130I CCWWGG 2 cut(s) 321, 1023
Eco24I GRGCYC 1 cut(s) 1364
Eco31I GGTCTC 1 cut(s) 884
Eco32I GATATC 3 cut(s) 933, 1882, 1950
Eco57I CTGAAG 2 cut(s) 452, 1388
Eco72I CACGTG 1 cut(s) 417
EcoRI GAATTC 2 cut(s) 619, 1531
EcoRII CCWGG 2 cut(s) 1822, 2064
EcoRV GATATC 3 cut(s) 933, 1882, 1950
EcoT14I CCWWGG 2 cut(s) 321, 1023
EcoT22I ATGCAT 2 cut(s) 443, 791
EcoT38I GRGCYC 1 cut(s) 1364
EgeI GGCGCC 1 cut(s) 1504
EheI GGCGCC 1 cut(s) 1504
ErhI CCWWGG 2 cut(s) 321, 1023
FalI AAGNNNNNCTT 2 cut(s) 1833, 1865
FaqI GGGAC 3 cut(s) 2034, 2153, 2244
FauI CCCGC 1 cut(s) 278
FblI GTMKAC 2 cut(s) 81, 1130
Fnu4HI GCNGC 2 cut(s) 1311, 2145
FokI GGATG 8 cut(s) 481, 604, 798, 1056, 1869, 1994, 2085, 2213
FriOI GRGCYC 1 cut(s) 1364
Fsp4HI GCNGC 2 cut(s) 1311, 2145
FspBI CTAG 5 cut(s) 800, 836, 1175, 1545, 1746
FspI TGCGCA 1 cut(s) 573
GlaI GCGC 3 cut(s) 429, 573, 1504
GluI GCNGC 2 cut(s) 1311, 2145
HaeII RGCGCY 1 cut(s) 1506
HaeIII GGCC 1 cut(s) 1229
HapII CCGG 7 cut(s) 1402, 1665, 1999, 2036, 2085, 2171, 2276
HgaI GACGC 1 cut(s) 1255
HhaI GCGC 3 cut(s) 430, 574, 1505
Hin1I GRCGYC 1 cut(s) 1503
Hin6I GCGC 3 cut(s) 428, 572, 1503
HinP1I GCGC 3 cut(s) 428, 572, 1503
HincII GTYRAC 1 cut(s) 2323
HindII GTYRAC 1 cut(s) 2323
HindIII AAGCTT 4 cut(s) 152, 727, 764, 2284
HpaII CCGG 7 cut(s) 1402, 1665, 1999, 2036, 2085, 2171, 2276
HphI GGTGA 4 cut(s) 559, 872, 1039, 2290
Hpy166II GTNNAC 7 cut(s) 82, 115, 241, 961, 1131, 1352, 2323
Hpy188I TCNGA 8 cut(s) 247, 905, 1290, 1368, 2027, 2060, 2103, 2261
Hpy188III TCNNGA 9 cut(s) 116, 928, 1017, 1402, 1665, 1838, 1999, 2008, 2192
Hpy8I GTNNAC 7 cut(s) 82, 115, 241, 961, 1131, 1352, 2323
HpyAV CCTTC 8 cut(s) 58, 82, 427, 456, 1594, 1643, 1941, 2206
HpyCH4III ACNGT 4 cut(s) 356, 1281, 1553, 2327
HpyCH4IV ACGT 4 cut(s) 416, 826, 1212, 2196
HpyF10VI GCNNNNNNNGC 5 cut(s) 434, 1255, 1268, 1773, 2153
HpyF3I CTNAG 6 cut(s) 56, 76, 725, 1482, 1791, 2024
HpySE526I ACGT 4 cut(s) 416, 826, 1212, 2196
Hsp92I GRCGYC 1 cut(s) 1503
HspAI GCGC 3 cut(s) 428, 572, 1503
KasI GGCGCC 1 cut(s) 1502
Kpn2I TCCGGA 3 cut(s) 1401, 1664, 1998
KroI GCCGGC 1 cut(s) 2275
KroNI GCCGGC 1 cut(s) 2277
Kzo9I GATC 8 cut(s) 183, 283, 511, 1111, 1398, 1711, 1756, 2295
LmnI GCTCC 1 cut(s) 2001
Lsp1109I GCAGC 2 cut(s) 1297, 2156
LweI GCATC 5 cut(s) 582, 776, 1281, 1972, 2374
MabI ACCWGGT 1 cut(s) 2064
MaeI CTAG 5 cut(s) 800, 836, 1175, 1545, 1746
MaeII ACGT 4 cut(s) 416, 826, 1212, 2196
MalI GATC 8 cut(s) 185, 285, 513, 1113, 1400, 1713, 1758, 2297
MboI GATC 8 cut(s) 183, 283, 511, 1111, 1398, 1711, 1756, 2295
MflI RGATCY 1 cut(s) 1756
MhlI GDGCHC 1 cut(s) 1364
Mly113I GGCGCC 1 cut(s) 1503
MlyI GAGTC 4 cut(s) 718, 974, 1039, 2016
MmeI TCCRAC 2 cut(s) 596, 2038
Mph1103I ATGCAT 2 cut(s) 443, 791
MroI TCCGGA 3 cut(s) 1401, 1664, 1998
MroNI GCCGGC 1 cut(s) 2275
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 9 cut(s) 192, 234, 375, 480, 702, 747, 1803, 1851, 2267
MslI CAYNNNNRTG 3 cut(s) 1089, 1563, 1975
MspA1I CMGCKG 2 cut(s) 1058, 1898
MspI CCGG 7 cut(s) 1402, 1665, 1999, 2036, 2085, 2171, 2276
MspR9I CCNGG 4 cut(s) 1824, 2037, 2066, 2171
Mva1269I GAATGC 2 cut(s) 942, 2255
MvaI CCWGG 2 cut(s) 1824, 2066
MwoI GCNNNNNNNGC 5 cut(s) 434, 1255, 1268, 1773, 2153
NaeI GCCGGC 1 cut(s) 2277
NarI GGCGCC 1 cut(s) 1503
NciI CCSGG 2 cut(s) 2037, 2171
NcoI CCATGG 2 cut(s) 321, 1023
NdeII GATC 8 cut(s) 183, 283, 511, 1111, 1398, 1711, 1756, 2295
NgoMIV GCCGGC 1 cut(s) 2275
NheI GCTAGC 1 cut(s) 1174
NlaIV GGNNCC 3 cut(s) 1021, 1504, 2003
NmeAIII GCCGAG 2 cut(s) 732, 1793
NmuCI GTSAC 7 cut(s) 126, 860, 991, 1658, 2192, 2327, 2335
NsbI TGCGCA 1 cut(s) 573
NsiI ATGCAT 2 cut(s) 443, 791
NspI RCATGY 3 cut(s) 441, 868, 1262
PaeI GCATGC 2 cut(s) 441, 1262
PagI TCATGA 2 cut(s) 927, 2007
PctI GAATGC 2 cut(s) 942, 2255
PdiI GCCGGC 1 cut(s) 2277
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 6 cut(s) 535, 733, 1013, 1919, 1927, 2305
PflMI CCANNNNNTGG 3 cut(s) 217, 988, 1702
PfoI TCCNGGA 1 cut(s) 2169
PkrI GCNGC 2 cut(s) 1312, 2146
PleI GAGTC 4 cut(s) 717, 973, 1038, 2016
PluTI GGCGCC 1 cut(s) 1506
PmaCI CACGTG 1 cut(s) 417
PmlI CACGTG 1 cut(s) 417
PpsI GAGTC 4 cut(s) 717, 973, 1038, 2016
Ppu21I YACGTR 2 cut(s) 417, 827
PshBI ATTAAT 1 cut(s) 192
Psp6I CCWGG 2 cut(s) 1822, 2064
PspCI CACGTG 1 cut(s) 417
PspGI CCWGG 2 cut(s) 1822, 2064
PspN4I GGNNCC 3 cut(s) 1021, 1504, 2003
PsuI RGATCY 1 cut(s) 1756
PvuII CAGCTG 2 cut(s) 1058, 1898
RsaI GTAC 6 cut(s) 223, 242, 353, 829, 960, 1151
RsaNI GTAC 6 cut(s) 222, 241, 352, 828, 959, 1150
RseI CAYNNNNRTG 3 cut(s) 1089, 1563, 1975
SaqAI TTAA 9 cut(s) 192, 234, 375, 480, 702, 747, 1803, 1851, 2267
SatI GCNGC 2 cut(s) 1311, 2145
Sau3AI GATC 8 cut(s) 183, 283, 511, 1111, 1398, 1711, 1756, 2295
SchI GAGTC 4 cut(s) 718, 974, 1039, 2016
ScrFI CCNGG 4 cut(s) 1824, 2037, 2066, 2171
SduI GDGCHC 1 cut(s) 1364
SexAI ACCWGGT 1 cut(s) 2064
SfaNI GCATC 5 cut(s) 582, 776, 1281, 1972, 2374
SfcI CTRYAG 1 cut(s) 1588
SfoI GGCGCC 1 cut(s) 1504
SmiMI CAYNNNNRTG 3 cut(s) 1089, 1563, 1975
SmlI CTYRAG 2 cut(s) 365, 1965
SmoI CTYRAG 2 cut(s) 365, 1965
SnaBI TACGTA 1 cut(s) 827
SphI GCATGC 2 cut(s) 441, 1262
SsiI CCGC 1 cut(s) 271
SspDI GGCGCC 1 cut(s) 1502
SspMI CTAG 5 cut(s) 800, 836, 1175, 1545, 1746
StyD4I CCNGG 4 cut(s) 1822, 2035, 2064, 2169
StyI CCWWGG 2 cut(s) 321, 1023
TaaI ACNGT 4 cut(s) 356, 1281, 1553, 2327
TaiI ACGT 4 cut(s) 419, 829, 1215, 2199
TaqI TCGA 5 cut(s) 282, 514, 530, 585, 2409
TatI WGTACW 2 cut(s) 240, 1149
TfiI GAWTC 6 cut(s) 535, 733, 1013, 1919, 1927, 2305
Tru1I TTAA 9 cut(s) 192, 234, 375, 480, 702, 747, 1803, 1851, 2267
Tru9I TTAA 9 cut(s) 192, 234, 375, 480, 702, 747, 1803, 1851, 2267
TscAI CASTG 2 cut(s) 361, 2154
TseFI GTSAC 7 cut(s) 126, 860, 991, 1658, 2192, 2327, 2335
TseI GCWGC 2 cut(s) 1310, 2144
Tsp45I GTSAC 7 cut(s) 126, 860, 991, 1658, 2192, 2327, 2335
TspGWI ACGGA 3 cut(s) 42, 1575, 1987
TspRI CASTG 2 cut(s) 361, 2154
Van91I CCANNNNNTGG 3 cut(s) 217, 988, 1702
VspI ATTAAT 1 cut(s) 192
XapI RAATTY 4 cut(s) 213, 619, 757, 1531
XceI RCATGY 3 cut(s) 441, 868, 1262
XmiI GTMKAC 2 cut(s) 81, 1130
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 5 cut(s) 800, 836, 1175, 1545, 1746
Zsp2I ATGCAT 2 cut(s) 443, 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.