Rw3G003440

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0016866
Physical Location & Seq
Forward (+)
2936417 .. 2938883
2467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G003440.1

Sequence Viewer

Length: 2430 bp
ATGCGTTGTCTCTTCCTTCTTCTCCATCGCTTTGTGCCCAATACACCCTTCAAGCTCAGCCCCTCATCTTCCTTCTCAGCCTATGCTTCTTCTTCTTCAATCAAATTTCCCAACTTCAACCAATCCCAACCTAAAGTTCAAAACTTTTTGTCACTCTTGCAACGATTTTCGAAAAATCTCATCTGGGTCAACTCCATTCATGCCCAGATCATCACCAATGCGCTATTCACAGACCAAAGTTTGGTCTCAAAGCTTGTTAGAGTGTACTGCGACTTGGGTAGTTTGGTTTATGCACGCCACGTGTTTGATGAATGTCCTCACCCAAAAACCATTCTTTACAATTCTATGATTTATGGGTATTTGAGAAATGAGAGGTACAATGAGGCCCTTGAGTTGTTTAGATTTATGGGTTCTTGTAATGTGGAGGTGGATAGTTATACATGTAACTATGCGCTCAAGGCGTGCATGGGGCTTTCGGATTATGATACGGGTGTGGAGGTTATGAAGAGAGCTGTGGATAAAGGGGTTGAAAGCAATGGGTTCTTGGGGAGTTCAATGATAAACTTTTTGATGAAATTCGGTAATGTTGATGAGGCGCAGAGGTGTTTTGATAGGATGCCTGAAAGAGATGTTGTTTGTTGGAATTCGATGATTGGTGGCTATGTGCAAGCTGGTCAGTTCGGCAAGGCATTTGATTTGTTTCATGAAATGTGTGATTGCGGCATTAGGCCTAGTTCAGTGACTATGCTGAGCATGATTCAAGTATGCATTAAAACTGCGGATTTCAAGCTTGCGAAATGTGTTCATGGTAGCATAGTTGGACTGGGAATGAGCAATGAGGTTTCGGTGCTTACCTCGTTGGTTGATATGTATAGTAATATTGGTGACGTGCTTAGTGCAGGTTGGGTTTTTGAGACCATGCCCAAAAGAAATTTGGTTTCGTGGAATGTCATGATCTCAGGATGTGTTCAAAATGGTATGGTGCATGAATCGTTTGCCTTCTTTGGTAGATTAGTTGCAAGTGGTGTTAGGTTTGATTCAGGGACCATGGTGAGCCTAATTCAGGGTTGTTCCCAAACAGCTGATTTGAAAGGTGGAAAAATCCTACACTGTTGTTCATTAAGGAGGGGTTTTGACTTAAATCCCATTTTGTCCACTGCCACTGTTGATCTTTACTCTAAATGTGGTGCAGTAAAGCAGGCAACTTTTGTCTTTGACAGAATGGAAGAGAGGAATGTTATTACATGGACCGCTATGCTTGTGGGACTGACACAAAATGGGCATGCAGAAGAAGCCTTGAAGCTATTCTGTCAGATGCAAAAAGAGGGAGTTGCTGCCAATTCAGTGACACTGGTTAGTTTAGTCCATTCTTGTGCCCATTTGGGCTCTCTGAAGAAAGGAAAGAGTGTTCATGCTCACATAATCCGGCAGGGCCATTCATTTGACATAGTTAACATGACATCCTTAATTGATATGTATGCCAAGTGTGGAACAATAAAGTATGCTGAGAGGATATTTAACAATGGCTCCATTTGTAGAGATGTTATCCTATGGAACTCTATGATAACAAGCTATGGTATTCATGGTCATGGACAGCAAGCTCTTGGTATGTATAGAAAAATGAAGGAGGAGGGATTTGAGCCAAACGAAACCTCCTTTCTTTCTCTTCTAACTGCTTGTAGTCACTCAGGACTTCTTGAAGAGGGCATCAAGTTTTTCCACAGCATGGAGAGAAACCATAACATTAGGCTGACAGAAAAACACTATGCAAGTTTTGTGGATCTTCTAAGCAGAGCAGGCAGATTTGAAGAAGCTGAGGCATTGATTGAACAAATGCCTTTTAAACCAGGAAGCTCTGTTTTTGAAGCATTGCTCAGTGGATGTCAAGCTCACAAGAACATTGAACTGGGTCTAAAGACGGCAGATAGATTACTTTGTATTGATTCCATGAATCCTAGTGTTTATGTCGTTGTTTCAAATATTTATGCTCAAGCAAGGAGATGGGATGATGTGAACTACATTCGAAACCTCATGAGGACAAGAGGACTAAGGAAAACACCCGGGTGTAGCTTGATTGAAGTGGGAAACCAGGTCCACACATTCTTCGCTGGAGACAATTCACATCCTAATTGGGCAGAGATTTATCAGCATTTAGAGAACATAAGAGTAGAAGTGGAGGCTTCTGGTTATATCCCCAATACAAGGCAGCTGCTATGGGGGCACAGCGAGAGATTAGCCATTGCATTTGGGCTCTTAAGTACACCAGCTGGGAGCTTGATTAGAATCACAAAGAATCTAAGAGTGTGTGTTGATTGTCACAATGTGACTAAACTTATATCAAAGATGATGAGGAGGGAGCTTATTGTGAGAGATGCCAATCGTTTTCATCACTTTGTGAATGGGAAGTGCTCTTGTAATGATTACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

809

Amino Acids

90.83

Weight (kDa)

8.51

Isoelectric Point (pI)

35.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 108 - 155 1.1e-09 PPR repeat family
PPR_2 PF13041 209 - 257 4.5e-14 PPR repeat family
PPR PF01535 212 - 242 5.6e-09 PPR repeat
PPR_2 PF13041 311 - 358 3.4e-07 PPR repeat family
PPR PF01535 313 - 343 3e-06 PPR repeat
PPR_2 PF13041 411 - 460 1.6e-11 PPR repeat family
PPR PF01535 414 - 444 2.8e-07 PPR repeat
PPR_2 PF13041 513 - 561 1.2e-10 PPR repeat family
PPR PF01535 517 - 546 3.8e-06 PPR repeat
E_motif PF20431 631 - 693 6.7e-20 E motif
Eplus_motif PF20430 698 - 721 4e-07 E+ motif
DYW_deaminase PF14432 735 - 809 2.9e-36 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016866)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04050
malus_domestica MD04G1216800.v1.1
prunus_persica Prupe.6G336500_v2.0.a1
pyrus_communis pycom04g19280
rosa_chinensis RchiOBHm_Chr3g0452611
rosa_laevigata RLG00000025578
rosa_multiflora Rmu_sc0011540.1_g000002 Rmu_sc0034042.1_g000001
rosa_roxburghii Rroxscaffold_6G00429650
rosa_rugosa Rorug02G0641700
rosa_samantha Rh3AG044200 Rh3BG046500 Rh3DG045500
rosa_wichuraiana Rw3G003440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 890
AccB7I CCANNNNNTGG 2 cut(s) 241, 1726
AciI CCGC 3 cut(s) 720, 779, 1251
AclWI GGATC 1 cut(s) 1788
AcsI RAATTY 4 cut(s) 104, 575, 643, 931
AcuI CTGAAG 1 cut(s) 1412
AcvI CACGTG 1 cut(s) 301
AdeI CACNNNGTG 2 cut(s) 2323, 2395
AfaI GTAC 3 cut(s) 266, 377, 2260
AfiI CCNNNNNNNGG 4 cut(s) 241, 1063, 1726, 2202
AflII CTTAAG 1 cut(s) 2254
AflIII ACRYGT 2 cut(s) 300, 440
AjiI CACGTC 1 cut(s) 889
AjnI CCWGG 2 cut(s) 1846, 2088
AjuI GAANNNNNNNTTGG 6 cut(s) 32, 64, 120, 152, 209, 241
AleI CACNNNNGTG 1 cut(s) 2062
AloI GAACNNNNNNTCC 2 cut(s) 1392, 1424
Alw21I GWGCWC 1 cut(s) 2411
Alw26I GTCTC 4 cut(s) 14, 250, 908, 2106
AlwI GGATC 1 cut(s) 1788
Ama87I CYCGRG 1 cut(s) 2060
AoxI GGCC 3 cut(s) 384, 728, 1432
ApeKI GCWGC 3 cut(s) 1334, 2206, 2209
ApoI RAATTY 4 cut(s) 104, 575, 643, 931
Asp700I GAANNNNTTC 1 cut(s) 1304
AspLEI GCGC 3 cut(s) 223, 454, 598
AspS9I GGNCC 5 cut(s) 385, 1044, 1248, 1432, 2092
AsuC2I CCSGG 2 cut(s) 2061, 2062
AsuHPI GGTGA 4 cut(s) 205, 311, 896, 1063
AsuII TTCGAA 2 cut(s) 170, 2023
AvaI CYCGRG 1 cut(s) 2060
AvaII GGWCC 3 cut(s) 1044, 1248, 2092
BaeGI GKGCMC 3 cut(s) 39, 1378, 2223
BaeI ACNNNNGTAYC 2 cut(s) 477, 510
BanII GRGCYC 2 cut(s) 1388, 2253
BbrPI CACGTG 1 cut(s) 301
Bbv12I GWGCWC 1 cut(s) 2411
BbvCI CCTCAGC 1 cut(s) 1815
BbvI GCAGC 3 cut(s) 1321, 2196, 2218
BccI CCATC 2 cut(s) 33, 1995
BceAI ACGGC 1 cut(s) 1935
BciT130I CCWGG 2 cut(s) 1848, 2090
BcnI CCSGG 2 cut(s) 2061, 2062
BcoDI GTCTC 4 cut(s) 14, 250, 908, 2106
BfaI CTAG 2 cut(s) 732, 1956
BfrI CTTAAG 1 cut(s) 2254
BfuAI ACCTGC 1 cut(s) 890
BisI GCNGC 4 cut(s) 721, 1335, 2207, 2210
BlpI GCTNAGC 2 cut(s) 56, 749
BlsI GCNGC 4 cut(s) 722, 1336, 2208, 2211
Bme1390I CCNGG 4 cut(s) 1848, 2061, 2062, 2090
Bme18I GGWCC 3 cut(s) 1044, 1248, 2092
BmeT110I CYCGRG 1 cut(s) 2060
BmgBI CACGTC 1 cut(s) 889
BmgT120I GGNCC 5 cut(s) 385, 1044, 1248, 1432, 2092
BmiI GGNNCC 2 cut(s) 1045, 1528
BmrFI CCNGG 4 cut(s) 1848, 2061, 2062, 2090
BmrI ACTGGG 2 cut(s) 833, 1916
BmsI GCATC 4 cut(s) 606, 1305, 1716, 2362
BmuI ACTGGG 2 cut(s) 833, 1916
BpmI CTGGAG 1 cut(s) 2130
Bpu10I CCTNAGC 1 cut(s) 1815
Bpu1102I GCTNAGC 2 cut(s) 56, 749
Bpu14I TTCGAA 2 cut(s) 170, 2023
BpuEI CTTGAG 3 cut(s) 410, 440, 1974
BpuMI CCSGG 2 cut(s) 2061, 2062
BsaAI YACGTR 1 cut(s) 301
BsaBI GATNNNNATC 2 cut(s) 2282, 2376
BsaI GGTCTC 2 cut(s) 250, 908
BsaJI CCNNGG 2 cut(s) 1047, 2060
BsaXI ACNNNNNCTCC 4 cut(s) 1511, 1541, 1637, 1667
Bsc4I CCNNNNNNNGG 4 cut(s) 241, 1063, 1726, 2202
Bse1I ACTGG 4 cut(s) 828, 1356, 1911, 2429
Bse3DI GCAATG 4 cut(s) 541, 841, 1868, 2238
Bse8I GATNNNNATC 2 cut(s) 2282, 2376
BseBI CCWGG 2 cut(s) 1848, 2090
BseDI CCNNGG 2 cut(s) 1047, 2060
BseGI GGATG 6 cut(s) 621, 968, 1460, 1886, 2011, 2122
BseJI GATNNNNATC 2 cut(s) 2282, 2376
BseLI CCNNNNNNNGG 4 cut(s) 241, 1063, 1726, 2202
BseMI GCAATG 4 cut(s) 541, 841, 1868, 2238
BseMII CTCAG 8 cut(s) 70, 90, 740, 972, 1497, 1701, 1806, 1888
BseNI ACTGG 4 cut(s) 828, 1356, 1911, 2429
BseRI GAGGAG 2 cut(s) 1643, 2365
BseSI GKGCMC 3 cut(s) 39, 1378, 2223
BseXI GCAGC 3 cut(s) 1321, 2196, 2218
BseYI CCCAGC 1 cut(s) 2267
BsgI GTGCAG 2 cut(s) 918, 1209
BshFI GGCC 3 cut(s) 386, 730, 1434
BsiHKAI GWGCWC 1 cut(s) 2411
BsiHKCI CYCGRG 1 cut(s) 2060
BsiSI CCGG 2 cut(s) 1426, 2061
BslFI GGGAC 2 cut(s) 1057, 1278
BslI CCNNNNNNNGG 4 cut(s) 241, 1063, 1726, 2202
BsmAI GTCTC 4 cut(s) 14, 250, 908, 2106
BsmFI GGGAC 2 cut(s) 1057, 1278
BsnI GGCC 3 cut(s) 386, 730, 1434
Bso31I GGTCTC 2 cut(s) 250, 908
BsoBI CYCGRG 1 cut(s) 2060
Bsp119I TTCGAA 2 cut(s) 170, 2023
Bsp1286I GDGCHC 6 cut(s) 39, 1378, 1388, 2223, 2253, 2411
Bsp143I GATC 4 cut(s) 207, 954, 1168, 1780
Bsp1720I GCTNAGC 2 cut(s) 56, 749
Bsp19I CCATGG 1 cut(s) 1047
BspACI CCGC 3 cut(s) 720, 779, 1251
BspANI GGCC 3 cut(s) 386, 730, 1434
BspCNI CTCAG 8 cut(s) 69, 89, 741, 971, 1498, 1700, 1807, 1887
BspHI TCATGA 3 cut(s) 703, 951, 2031
BspLI GGNNCC 2 cut(s) 1045, 1528
BspMI ACCTGC 1 cut(s) 890
BspPI GGATC 1 cut(s) 1788
BspT104I TTCGAA 2 cut(s) 170, 2023
BspTI CTTAAG 1 cut(s) 2254
BspTNI GGTCTC 2 cut(s) 250, 908
BsrDI GCAATG 4 cut(s) 541, 841, 1868, 2238
BsrI ACTGG 4 cut(s) 828, 1356, 1911, 2429
BssECI CCNNGG 2 cut(s) 1047, 2060
BssMI GATC 4 cut(s) 207, 954, 1168, 1780
BssT1I CCWWGG 1 cut(s) 1047
Bst2UI CCWGG 2 cut(s) 1848, 2090
Bst4CI ACNGT 2 cut(s) 1112, 1165
Bst6I CTCTTC 5 cut(s) 17, 500, 1221, 1671, 1695
BstAFI CTTAAG 1 cut(s) 2254
BstBAI YACGTR 1 cut(s) 301
BstBI TTCGAA 2 cut(s) 170, 2023
BstC8I GCNNGC 8 cut(s) 295, 463, 669, 792, 1200, 1284, 1599, 1798
BstDSI CCRYGG 1 cut(s) 1047
BstENI CCTNNNNNAGG 1 cut(s) 1061
BstF5I GGATG 6 cut(s) 621, 968, 1460, 1886, 2011, 2122
BstHHI GCGC 3 cut(s) 223, 454, 598
BstKTI GATC 4 cut(s) 210, 957, 1171, 1783
BstMAI GTCTC 4 cut(s) 14, 250, 908, 2106
BstMBI GATC 4 cut(s) 207, 954, 1168, 1780
BstMWI GCNNNNNNNGC 4 cut(s) 458, 1292, 1797, 2218
BstNI CCWGG 2 cut(s) 1848, 2090
BstNSI RCATGY 2 cut(s) 444, 1286
BstSCI CCNGG 4 cut(s) 1846, 2059, 2060, 2088
BstSLI GKGCMC 3 cut(s) 39, 1378, 2223
BstV1I GCAGC 3 cut(s) 1321, 2196, 2218
BstX2I RGATCY 1 cut(s) 1780
BstYI RGATCY 1 cut(s) 1780
BsuRI GGCC 3 cut(s) 386, 730, 1434
BtgI CCRYGG 1 cut(s) 1047
BtgZI GCGATG 1 cut(s) 11
BtrI CACGTC 1 cut(s) 889
BtsCI GGATG 6 cut(s) 621, 968, 1460, 1886, 2011, 2122
BtsI GCAGTG 1 cut(s) 1155
BtsIMutI CAGTG 7 cut(s) 744, 1108, 1155, 1161, 1349, 1350, 1882
BveI ACCTGC 1 cut(s) 890
Cac8I GCNNGC 8 cut(s) 295, 463, 669, 792, 1200, 1284, 1599, 1798
CciI TCATGA 3 cut(s) 703, 951, 2031
CfoI GCGC 3 cut(s) 223, 454, 598
Cfr13I GGNCC 5 cut(s) 385, 1044, 1248, 1432, 2092
Cfr9I CCCGGG 1 cut(s) 2060
CsiI ACCWGGT 1 cut(s) 2088
Csp6I GTAC 3 cut(s) 265, 376, 2259
CspCI CAANNNNNGTGG 2 cut(s) 1758, 1793
CviQI GTAC 3 cut(s) 265, 376, 2259
DpnI GATC 4 cut(s) 209, 956, 1170, 1782
DpnII GATC 4 cut(s) 207, 954, 1168, 1780
DraI TTTAAA 1 cut(s) 1843
DraIII CACNNNGTG 2 cut(s) 2323, 2395
Eam1104I CTCTTC 5 cut(s) 17, 500, 1221, 1671, 1695
EarI CTCTTC 5 cut(s) 17, 500, 1221, 1671, 1695
Eco130I CCWWGG 1 cut(s) 1047
Eco147I AGGCCT 1 cut(s) 730
Eco24I GRGCYC 2 cut(s) 1388, 2253
Eco31I GGTCTC 2 cut(s) 250, 908
Eco47I GGWCC 3 cut(s) 1044, 1248, 2092
Eco57I CTGAAG 1 cut(s) 1412
Eco72I CACGTG 1 cut(s) 301
Eco88I CYCGRG 1 cut(s) 2060
EcoNI CCTNNNNNAGG 1 cut(s) 1061
EcoO109I RGGNCCY 1 cut(s) 385
EcoRI GAATTC 1 cut(s) 643
EcoRII CCWGG 2 cut(s) 1846, 2088
EcoT14I CCWWGG 1 cut(s) 1047
EcoT22I ATGCAT 1 cut(s) 770
EcoT38I GRGCYC 2 cut(s) 1388, 2253
ErhI CCWWGG 1 cut(s) 1047
FaqI GGGAC 2 cut(s) 1057, 1278
Fnu4HI GCNGC 4 cut(s) 721, 1335, 2207, 2210
FokI GGATG 6 cut(s) 628, 975, 1447, 1893, 2018, 2109
FriOI GRGCYC 2 cut(s) 1388, 2253
Fsp4HI GCNGC 4 cut(s) 721, 1335, 2207, 2210
FspBI CTAG 2 cut(s) 732, 1956
GlaI GCGC 3 cut(s) 222, 453, 597
GluI GCNGC 4 cut(s) 721, 1335, 2207, 2210
GsaI CCCAGC 1 cut(s) 2271
GsuI CTGGAG 1 cut(s) 2130
HaeIII GGCC 3 cut(s) 386, 730, 1434
HapII CCGG 2 cut(s) 1426, 2061
HhaI GCGC 3 cut(s) 223, 454, 598
Hin6I GCGC 3 cut(s) 221, 452, 596
HinP1I GCGC 3 cut(s) 221, 452, 596
HincII GTYRAC 2 cut(s) 190, 1453
HindII GTYRAC 2 cut(s) 190, 1453
HindIII AAGCTT 2 cut(s) 251, 788
HinfI GANTC 7 cut(s) 757, 989, 1037, 1943, 1951, 2283, 2293
HpaI GTTAAC 1 cut(s) 1453
HpaII CCGG 2 cut(s) 1426, 2061
HphI GGTGA 4 cut(s) 205, 311, 896, 1063
Hpy166II GTNNAC 7 cut(s) 190, 265, 1155, 1453, 2014, 2095, 2261
Hpy188I TCNGA 3 cut(s) 478, 1314, 1392
Hpy188III TCNNGA 6 cut(s) 704, 952, 960, 1689, 1697, 2032
Hpy8I GTNNAC 7 cut(s) 190, 265, 1155, 1453, 2014, 2095, 2261
HpyAV CCTTC 5 cut(s) 26, 58, 82, 1009, 1618
HpyCH4III ACNGT 2 cut(s) 1112, 1165
HpyCH4IV ACGT 2 cut(s) 300, 888
HpyF10VI GCNNNNNNNGC 4 cut(s) 458, 1292, 1797, 2218
HpySE526I ACGT 2 cut(s) 300, 888
HspAI GCGC 3 cut(s) 221, 452, 596
KspAI GTTAAC 1 cut(s) 1453
Kzo9I GATC 4 cut(s) 207, 954, 1168, 1780
LmnI GCTCC 3 cut(s) 1532, 2271, 2356
Lsp1109I GCAGC 3 cut(s) 1321, 2196, 2218
LweI GCATC 4 cut(s) 606, 1305, 1716, 2362
MabI ACCWGGT 1 cut(s) 2088
MaeI CTAG 2 cut(s) 732, 1956
MaeII ACGT 2 cut(s) 300, 888
MaeIII GTNAC 8 cut(s) 150, 443, 739, 884, 1345, 1682, 2315, 2323
MalI GATC 4 cut(s) 209, 956, 1170, 1782
MboI GATC 4 cut(s) 207, 954, 1168, 1780
MflI RGATCY 1 cut(s) 1780
MhlI GDGCHC 6 cut(s) 39, 1378, 1388, 2223, 2253, 2411
MmeI TCCRAC 2 cut(s) 620, 799
Mph1103I ATGCAT 1 cut(s) 770
MroXI GAANNNNTTC 1 cut(s) 1304
MseI TTAA 8 cut(s) 771, 1121, 1139, 1452, 1466, 1518, 1842, 2255
MslI CAYNNNNRTG 3 cut(s) 1371, 1587, 2062
MspA1I CMGCKG 3 cut(s) 1082, 2209, 2267
MspCI CTTAAG 1 cut(s) 2254
MspI CCGG 2 cut(s) 1426, 2061
MspR9I CCNGG 4 cut(s) 1848, 2061, 2062, 2090
MvaI CCWGG 2 cut(s) 1848, 2090
MwoI GCNNNNNNNGC 4 cut(s) 458, 1292, 1797, 2218
NciI CCSGG 2 cut(s) 2061, 2062
NcoI CCATGG 1 cut(s) 1047
NdeII GATC 4 cut(s) 207, 954, 1168, 1780
NlaIV GGNNCC 2 cut(s) 1045, 1528
NmuCI GTSAC 7 cut(s) 150, 739, 884, 1345, 1682, 2315, 2323
NsiI ATGCAT 1 cut(s) 770
NspI RCATGY 2 cut(s) 444, 1286
NspV TTCGAA 2 cut(s) 170, 2023
OliI CACNNNNGTG 1 cut(s) 2062
PaeI GCATGC 1 cut(s) 1286
PagI TCATGA 3 cut(s) 703, 951, 2031
PceI AGGCCT 1 cut(s) 730
PciI ACATGT 1 cut(s) 440
PdmI GAANNNNTTC 1 cut(s) 1304
PfeI GAWTC 7 cut(s) 757, 989, 1037, 1943, 1951, 2283, 2293
PflMI CCANNNNNTGG 2 cut(s) 241, 1726
PkrI GCNGC 4 cut(s) 722, 1336, 2208, 2211
PmaCI CACGTG 1 cut(s) 301
PmlI CACGTG 1 cut(s) 301
Ppu21I YACGTR 1 cut(s) 301
PscI ACATGT 1 cut(s) 440
Psp6I CCWGG 2 cut(s) 1846, 2088
PspCI CACGTG 1 cut(s) 301
PspFI CCCAGC 1 cut(s) 2267
PspGI CCWGG 2 cut(s) 1846, 2088
PspN4I GGNNCC 2 cut(s) 1045, 1528
PspPI GGNCC 5 cut(s) 385, 1044, 1248, 1432, 2092
PsuI RGATCY 1 cut(s) 1780
PvuII CAGCTG 3 cut(s) 1082, 2209, 2267
RsaI GTAC 3 cut(s) 266, 377, 2260
RsaNI GTAC 3 cut(s) 265, 376, 2259
RseI CAYNNNNRTG 3 cut(s) 1371, 1587, 2062
SaqAI TTAA 8 cut(s) 771, 1121, 1139, 1452, 1466, 1518, 1842, 2255
SatI GCNGC 4 cut(s) 721, 1335, 2207, 2210
Sau3AI GATC 4 cut(s) 207, 954, 1168, 1780
Sau96I GGNCC 5 cut(s) 385, 1044, 1248, 1432, 2092
ScrFI CCNGG 4 cut(s) 1848, 2061, 2062, 2090
SduI GDGCHC 6 cut(s) 39, 1378, 1388, 2223, 2253, 2411
SexAI ACCWGGT 1 cut(s) 2088
SfaNI GCATC 4 cut(s) 606, 1305, 1716, 2362
SfuI TTCGAA 2 cut(s) 170, 2023
SinI GGWCC 3 cut(s) 1044, 1248, 2092
SmaI CCCGGG 1 cut(s) 2062
SmiMI CAYNNNNRTG 3 cut(s) 1371, 1587, 2062
SmlI CTYRAG 4 cut(s) 389, 455, 1989, 2254
SmoI CTYRAG 4 cut(s) 389, 455, 1989, 2254
SphI GCATGC 1 cut(s) 1286
SseBI AGGCCT 1 cut(s) 730
SsiI CCGC 3 cut(s) 720, 779, 1251
SspI AATATT 2 cut(s) 880, 1981
SspMI CTAG 2 cut(s) 732, 1956
StuI AGGCCT 1 cut(s) 730
StyD4I CCNGG 4 cut(s) 1846, 2059, 2060, 2088
StyI CCWWGG 1 cut(s) 1047
TaaI ACNGT 2 cut(s) 1112, 1165
TaiI ACGT 2 cut(s) 303, 891
TaqI TCGA 3 cut(s) 170, 647, 2023
TatI WGTACW 2 cut(s) 264, 2258
TauI GCSGC 1 cut(s) 723
TfiI GAWTC 7 cut(s) 757, 989, 1037, 1943, 1951, 2283, 2293
Tru1I TTAA 8 cut(s) 771, 1121, 1139, 1452, 1466, 1518, 1842, 2255
Tru9I TTAA 8 cut(s) 771, 1121, 1139, 1452, 1466, 1518, 1842, 2255
TscAI CASTG 7 cut(s) 744, 1115, 1162, 1168, 1350, 1356, 1882
TseFI GTSAC 7 cut(s) 150, 739, 884, 1345, 1682, 2315, 2323
TseI GCWGC 3 cut(s) 1334, 2206, 2209
Tsp45I GTSAC 7 cut(s) 150, 739, 884, 1345, 1682, 2315, 2323
TspMI CCCGGG 1 cut(s) 2060
TspRI CASTG 7 cut(s) 744, 1115, 1162, 1168, 1350, 1356, 1882
Van91I CCANNNNNTGG 2 cut(s) 241, 1726
Vha464I CTTAAG 1 cut(s) 2254
VpaK11BI GGWCC 3 cut(s) 1044, 1248, 2092
XagI CCTNNNNNAGG 1 cut(s) 1061
XapI RAATTY 4 cut(s) 104, 575, 643, 931
XceI RCATGY 2 cut(s) 444, 1286
XcmI CCANNNNNNNNNTGG 1 cut(s) 931
XmaI CCCGGG 1 cut(s) 2060
XmnI GAANNNNTTC 1 cut(s) 1304
XspI CTAG 2 cut(s) 732, 1956
Zsp2I ATGCAT 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.