MD04G1216800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016866
Physical Location & Seq
Reverse (-)
29932730 .. 29935171
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1216800.v1.1.491

Sequence Viewer

Length: 2442 bp
ATGCGTAATCTGTTCCTCCATATCCACGGATTATTTCCCAAAATACCCTTCAATCTCAGCCCCTCGTCTTCCTTCTCAGCCTACGCTTCTTATTCTTATTCTTCTGCAAATGTTCACGAATTCTCGTCACTCTTAAAACAATTTGCACAAAACCTTATCTGGGTCAAATCCATTCATGCTCAGATCATCATTAATTCTATATCTAATGACCAAATTCTGGGTACAAAGCTCGTTAAAGCGTACTCGGATTTGGGAAGTTTGGATGATGCCCGCCAAGTGTTCGATCAATTTCCTCAACCAGAAACCATTCTATGCAATGCCATGGTTTATGGGTATTTGAGAAAAGAGAGGTACAATGAGAGCCTTGAGTTGTTTAGTTTGATGGGTTCTTGTCATTTGGAGATTGATAGTTATACGTGTAATTATGCGCTCAAGGCGTGCATGGGCTTATTGGATTACAGAAGGGGGATGGAAGTTATTAAGAGAGCTGTGGATAAAGGAATTGAAAGTGATCGATTCCTGGGGAGTTCAATGATTCATTTCTTGGTGAAGTTTGATAAGATTGATTGCGCGCAAAGGTTTTTCGATAGGATGCCCGAAAGAGATGTTGTTTGTTGGAATTCCATGATTGGTGGCTATGTGCAATCTGGTCAGTTTAGTAAGGCAGTTGATATGTTCATAGAAATGTGTAGTTGTGGGGTTAAGCCAAGTCTGGTTACTATGTTAAGCTTGATTCAAGTTTGCATTAAAAGTGGGGATTTCAAGCTTGCAAAATGTGTTCATGGATGCATAGTTAGACTGGGAATGGGTAATGAGGTTTTACTACATACCTCGCTGGTGGATATGTATAGTAATATGGGTGACATGCAAAGTTCCTTTTCTGTTTTTGAGACCATGCCCATCAGAAGTTTGGTTTCGTGGAATGTCATGATATCGGGGTGCATTCAAAATGGTCTGGTACACGAGTCTTTTGGTTTCTTCCACAGATTGGTCACAGATGGTGGTACGTTTGATTCGGGAACCATGGTGAGTCTTGTTCAGGGATGTTCTCAAACAGCTGATTTGGAAACTGGGAAAATCCTGCATTGTTGTGTTTTTAGGATGGGTTTTGATCCAAATCCTATTTTGTCTACTGCCATTGTTGATTTGTACTCTAAATGTGGTGCAATAAAGCACGCAACATTTGTCTTTGATAGAATGAAAGAGAAGAACGTGATTACATGGACGGCCATGCTGGTAGGATTGACGCAAAATGGGCATGCAGAAGAAGCCTTGAAACTGTTTAGTCAGATGCGAGAAGAGGGAATTGCTGCTAATTCAGTGACTCTTGTTAGTTTAGTCCATTCCTGTTCACATTTGGGCTCTCTGAAGAAAGGCAGGAGTGTTCATGCTCATTTGATCCGGAATGGGCATGAAGTTGATGTAGTTACCATGACAGCCTTGATTGACATGTATGCGAAGTGTGGAAAAATAAAGTATGCTGAGAGAACATTTGAAAATGGCGCCTTTTGTAGAGATGTTATCCTATGGAATTCTATGATAACTAGCTACGGTATTCATGGATGTGGACTGCAAGCTCTTGGTATCTATAGAAGAATGAAGGAGGAGGGGTATAAGCCAAATGGAACCTCCCTTCTTTCTCTTCTAACTGCTTGTAATCACTCTGGACTTGTGGAAGAGGGTATCGAATTATTCCACAGCGTGGAGAGAGACTGTGGCATTAGGCTGACTGAAAAACACTATGCTAGTTTTGTGGATCTTTTATGCCGAGCAGGCAGACTTGACGAAGCCGAGGCATTGATTAAACAAATGCCTTTCGAACCAGGAAGTTCTGTTTTTGAAGCATTGCTGAATGGATGTCAGGTTCACAAGGACATTGATATTGGTATAAAGACAGCGGATAGATTACTTTGTATAGATTCCATGAATCCTTCAATTTATGTTGTGATATCAAACATTTATGCTCAAGCACGGAGATGGGATGCTGTAAATTATATCCGAAACCTCATGAGGACAAGGGGACTCAGAAAAATACCGGGGTATAGCTTGATTGAAGTCGGAAACCAGGTTCATACATTTTTTGCAGGAGACAACTCTCATCCGAACTGGGCAGAGATATATCAGCATTTAGAGGATTTAAGAGCAGCAGTGGAGGCTTCTGGTTATGTCCCGGATACAAGTTGTGTTCTTCGTGACGTGGATGAGCCGATGAAGGTCAGGTTGCTATGGGGACATAGTGAGAGATTAGCCATTGCATTCGGACTTTTAAACACGCCCGCGGGAAGCTTGATTAGGATCACCAAGAATCTGCGTGTGTGTGTTGACTGTCACAACGTGACTAAATATATATCCAAGATAGTGAGGAGGGAACTTATTGTGAGAGATGCCAATCGTTTCCATCACTTTGTCGATGGGAAATGCTCTTGCAATGATTACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

814

Amino Acids

91.24

Weight (kDa)

7.31

Isoelectric Point (pI)

31.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 201 - 249 2.4e-12 PPR repeat family
PPR PF01535 204 - 234 1.4e-08 PPR repeat
PPR_2 PF13041 403 - 451 7.1e-11 PPR repeat family
PPR PF01535 406 - 436 2.2e-07 PPR repeat
PPR_2 PF13041 505 - 552 4.2e-06 PPR repeat family
E_motif PF20431 623 - 685 2.8e-16 E motif
Eplus_motif PF20430 690 - 713 3.8e-08 E+ motif
DYW_deaminase PF14432 721 - 813 4.8e-43 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016866)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04050
malus_domestica MD04G1216800.v1.1
prunus_persica Prupe.6G336500_v2.0.a1
pyrus_communis pycom04g19280
rosa_chinensis RchiOBHm_Chr3g0452611
rosa_laevigata RLG00000025578
rosa_multiflora Rmu_sc0011540.1_g000002 Rmu_sc0034042.1_g000001
rosa_roxburghii Rroxscaffold_6G00429650
rosa_rugosa Rorug02G0641700
rosa_samantha Rh3AG044200 Rh3BG046500 Rh3DG045500
rosa_wichuraiana Rw3G003440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1502
AccB7I CCANNNNNTGG 3 cut(s) 217, 988, 1702
AccI GTMKAC 1 cut(s) 1130
AccII CGCG 2 cut(s) 572, 2279
AccIII TCCGGA 1 cut(s) 1401
AciI CCGC 4 cut(s) 271, 1898, 2277, 2279
AclWI GGATC 4 cut(s) 1106, 1393, 1764, 2303
AcoI YGGCCR 1 cut(s) 1227
AcsI RAATTY 4 cut(s) 119, 213, 619, 1531
AcuI CTGAAG 1 cut(s) 1388
AcyI GRCGYC 1 cut(s) 1503
AdeI CACNNNGTG 2 cut(s) 1702, 2335
AfaI GTAC 6 cut(s) 223, 242, 353, 960, 1006, 1151
AfiI CCNNNNNNNGG 4 cut(s) 160, 217, 988, 1702
AflIII ACRYGT 2 cut(s) 416, 1449
AjiI CACGTC 1 cut(s) 2197
AjnI CCWGG 3 cut(s) 519, 1822, 2064
AjuI GAANNNNNNNTTGG 2 cut(s) 32, 64
AloI GAACNNNNNNTCC 4 cut(s) 1848, 1880, 2052, 2084
AluBI AGCT 9 cut(s) 229, 488, 729, 766, 1058, 1548, 1577, 2046, 2286
AluI AGCT 9 cut(s) 229, 488, 729, 766, 1058, 1548, 1577, 2046, 2286
Alw26I GTCTC 3 cut(s) 884, 1704, 2082
AlwI GGATC 4 cut(s) 1106, 1393, 1764, 2303
Aor13HI TCCGGA 1 cut(s) 1401
AoxI GGCC 1 cut(s) 1227
ApeKI GCWGC 2 cut(s) 1310, 2144
ApoI RAATTY 4 cut(s) 119, 213, 619, 1531
AseI ATTAAT 1 cut(s) 192
Asp700I GAANNNNTTC 1 cut(s) 306
AspLEI GCGC 4 cut(s) 430, 572, 574, 1505
AsuC2I CCSGG 2 cut(s) 2037, 2171
AsuHPI GGTGA 4 cut(s) 559, 872, 1039, 2290
AsuII TTCGAA 1 cut(s) 1818
BaeI ACNNNNGTAYC 2 cut(s) 950, 983
BanI GGYRCC 1 cut(s) 1502
BanII GRGCYC 1 cut(s) 1364
BauI CACGAG 1 cut(s) 962
BbsI GAAGAC 1 cut(s) 60
BbvI GCAGC 2 cut(s) 1297, 2156
BccI CCATC 8 cut(s) 376, 463, 908, 992, 1096, 1971, 2405, 2406
BceAI ACGGC 1 cut(s) 1242
BciT130I CCWGG 3 cut(s) 521, 1824, 2066
BciVI GTATCC 1 cut(s) 2167
BcnI CCSGG 2 cut(s) 2037, 2171
BcoDI GTCTC 3 cut(s) 884, 1704, 2082
BfaI CTAG 2 cut(s) 1545, 1746
BfmI CTRYAG 1 cut(s) 1588
BfoI RGCGCY 1 cut(s) 1506
BfuI GTATCC 1 cut(s) 2167
BglI GCCNNNNNGGC 1 cut(s) 1773
BisI GCNGC 2 cut(s) 1311, 2145
BlsI GCNGC 2 cut(s) 1312, 2146
Bme1390I CCNGG 5 cut(s) 521, 1824, 2037, 2066, 2171
BmgBI CACGTC 1 cut(s) 2197
BmiI GGNNCC 3 cut(s) 1021, 1504, 1627
BmrFI CCNGG 5 cut(s) 521, 1824, 2037, 2066, 2171
BmrI ACTGGG 3 cut(s) 809, 1080, 2116
BmsI GCATC 6 cut(s) 256, 582, 776, 1281, 1972, 2374
BmuI ACTGGG 3 cut(s) 809, 1080, 2116
BpiI GAAGAC 1 cut(s) 60
Bpu14I TTCGAA 1 cut(s) 1818
BpuEI CTTGAG 3 cut(s) 386, 416, 1950
BpuMI CCSGG 2 cut(s) 2037, 2171
Bsa29I ATCGAT 1 cut(s) 514
BsaAI YACGTR 1 cut(s) 417
BsaBI GATNNNNATC 3 cut(s) 1116, 2294, 2388
BsaHI GRCGYC 1 cut(s) 1503
BsaI GGTCTC 1 cut(s) 884
BsaJI CCNNGG 7 cut(s) 25, 321, 520, 1023, 1791, 2036, 2277
BsaWI WCCGGW 1 cut(s) 1401
BsaXI ACNNNNNCTCC 2 cut(s) 1595, 1625
Bsc4I CCNNNNNNNGG 4 cut(s) 160, 217, 988, 1702
Bse1I ACTGG 4 cut(s) 804, 1075, 2111, 2441
Bse3DI GCAATG 4 cut(s) 322, 1844, 2250, 2434
Bse8I GATNNNNATC 3 cut(s) 1116, 2294, 2388
BseAI TCCGGA 1 cut(s) 1401
BseBI CCWGG 3 cut(s) 521, 1824, 2066
BseCI ATCGAT 1 cut(s) 514
BseDI CCNNGG 7 cut(s) 25, 321, 520, 1023, 1791, 2036, 2277
BseJI GATNNNNATC 3 cut(s) 1116, 2294, 2388
BseLI CCNNNNNNNGG 4 cut(s) 160, 217, 988, 1702
BseMI GCAATG 4 cut(s) 322, 1844, 2250, 2434
BseMII CTCAG 5 cut(s) 70, 90, 194, 1473, 2038
BseNI ACTGG 4 cut(s) 804, 1075, 2111, 2441
BsePI GCGCGC 1 cut(s) 570
BseRI GAGGAG 2 cut(s) 1619, 2377
BseXI GCAGC 2 cut(s) 1297, 2156
Bsh1236I CGCG 2 cut(s) 572, 2279
BshFI GGCC 1 cut(s) 1229
BshNI GGYRCC 1 cut(s) 1502
BshVI ATCGAT 1 cut(s) 514
BsiSI CCGG 3 cut(s) 1402, 2036, 2171
BslFI GGGAC 3 cut(s) 2034, 2153, 2244
BslI CCNNNNNNNGG 4 cut(s) 160, 217, 988, 1702
BsmAI GTCTC 3 cut(s) 884, 1704, 2082
BsmFI GGGAC 3 cut(s) 2034, 2153, 2244
BsmI GAATGC 2 cut(s) 942, 2255
BsnI GGCC 1 cut(s) 1229
Bso31I GGTCTC 1 cut(s) 884
Bsp119I TTCGAA 1 cut(s) 1818
Bsp1286I GDGCHC 1 cut(s) 1364
Bsp13I TCCGGA 1 cut(s) 1401
Bsp143I GATC 7 cut(s) 183, 283, 511, 1111, 1398, 1756, 2295
Bsp19I CCATGG 2 cut(s) 321, 1023
BspACI CCGC 4 cut(s) 271, 1898, 2277, 2279
BspANI GGCC 1 cut(s) 1229
BspCNI CTCAG 5 cut(s) 69, 89, 193, 1474, 2037
BspDI ATCGAT 1 cut(s) 514
BspEI TCCGGA 1 cut(s) 1401
BspFNI CGCG 2 cut(s) 572, 2279
BspHI TCATGA 2 cut(s) 927, 2007
BspLI GGNNCC 3 cut(s) 1021, 1504, 1627
BspPI GGATC 4 cut(s) 1106, 1393, 1764, 2303
BspT104I TTCGAA 1 cut(s) 1818
BspT107I GGYRCC 1 cut(s) 1502
BspTNI GGTCTC 1 cut(s) 884
BsrDI GCAATG 4 cut(s) 322, 1844, 2250, 2434
BsrI ACTGG 4 cut(s) 804, 1075, 2111, 2441
BssECI CCNNGG 7 cut(s) 25, 321, 520, 1023, 1791, 2036, 2277
BssHII GCGCGC 1 cut(s) 570
BssMI GATC 7 cut(s) 183, 283, 511, 1111, 1398, 1756, 2295
BssNI GRCGYC 1 cut(s) 1503
BssSI CACGAG 1 cut(s) 962
BssT1I CCWWGG 2 cut(s) 321, 1023
Bst2BI CACGAG 1 cut(s) 962
Bst2UI CCWGG 3 cut(s) 521, 1824, 2066
Bst4CI ACNGT 4 cut(s) 1281, 1553, 1715, 2327
Bst6I CTCTTC 3 cut(s) 1293, 1647, 1671
BstACI GRCGYC 1 cut(s) 1503
BstBAI YACGTR 1 cut(s) 417
BstBI TTCGAA 1 cut(s) 1818
BstC8I GCNNGC 9 cut(s) 271, 439, 572, 768, 1176, 1260, 1575, 1774, 2277
BstDEI CTNAG 5 cut(s) 56, 76, 180, 1482, 2024
BstDSI CCRYGG 4 cut(s) 25, 321, 1023, 2277
BstFNI CGCG 2 cut(s) 572, 2279
BstH2I RGCGCY 1 cut(s) 1506
BstHHI GCGC 4 cut(s) 430, 572, 574, 1505
BstKTI GATC 7 cut(s) 186, 286, 514, 1114, 1401, 1759, 2298
BstMAI GTCTC 3 cut(s) 884, 1704, 2082
BstMBI GATC 7 cut(s) 183, 283, 511, 1111, 1398, 1756, 2295
BstMWI GCNNNNNNNGC 5 cut(s) 434, 1255, 1268, 1773, 2153
BstNI CCWGG 3 cut(s) 521, 1824, 2066
BstNSI RCATGY 3 cut(s) 868, 1262, 1453
BstSCI CCNGG 5 cut(s) 519, 1822, 2035, 2064, 2169
BstSFI CTRYAG 1 cut(s) 1588
BstUI CGCG 2 cut(s) 572, 2279
BstV1I GCAGC 2 cut(s) 1297, 2156
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 1756
BstYI RGATCY 1 cut(s) 1756
Bsu15I ATCGAT 1 cut(s) 514
BsuI GTATCC 1 cut(s) 2167
BsuRI GGCC 1 cut(s) 1229
BsuTUI ATCGAT 1 cut(s) 514
BtgI CCRYGG 4 cut(s) 25, 321, 1023, 2277
BtrI CACGTC 1 cut(s) 2197
BtsI GCAGTG 1 cut(s) 2154
BtsIMutI CAGTG 2 cut(s) 1326, 2154
Cac8I GCNNGC 9 cut(s) 271, 439, 572, 768, 1176, 1260, 1575, 1774, 2277
CciI TCATGA 2 cut(s) 927, 2007
CfoI GCGC 4 cut(s) 430, 572, 574, 1505
Cfr42I CCGCGG 1 cut(s) 2280
ClaI ATCGAT 1 cut(s) 514
CseI GACGC 1 cut(s) 1255
CsiI ACCWGGT 1 cut(s) 2064
Csp6I GTAC 6 cut(s) 222, 241, 352, 959, 1005, 1150
CviQI GTAC 6 cut(s) 222, 241, 352, 959, 1005, 1150
DdeI CTNAG 5 cut(s) 56, 76, 180, 1482, 2024
DinI GGCGCC 1 cut(s) 1504
DpnI GATC 7 cut(s) 185, 285, 513, 1113, 1400, 1758, 2297
DpnII GATC 7 cut(s) 183, 283, 511, 1111, 1398, 1756, 2295
DraI TTTAAA 1 cut(s) 2268
DraIII CACNNNGTG 2 cut(s) 1702, 2335
EaeI YGGCCR 1 cut(s) 1227
Eam1104I CTCTTC 3 cut(s) 1293, 1647, 1671
EarI CTCTTC 3 cut(s) 1293, 1647, 1671
Eco130I CCWWGG 2 cut(s) 321, 1023
Eco24I GRGCYC 1 cut(s) 1364
Eco31I GGTCTC 1 cut(s) 884
Eco32I GATATC 2 cut(s) 933, 1950
Eco57I CTGAAG 1 cut(s) 1388
EcoRI GAATTC 3 cut(s) 119, 619, 1531
EcoRII CCWGG 3 cut(s) 519, 1822, 2064
EcoRV GATATC 2 cut(s) 933, 1950
EcoT14I CCWWGG 2 cut(s) 321, 1023
EcoT22I ATGCAT 1 cut(s) 791
EcoT38I GRGCYC 1 cut(s) 1364
EgeI GGCGCC 1 cut(s) 1504
EheI GGCGCC 1 cut(s) 1504
ErhI CCWWGG 2 cut(s) 321, 1023
FaqI GGGAC 3 cut(s) 2034, 2153, 2244
FauI CCCGC 3 cut(s) 278, 2272, 2284
FblI GTMKAC 1 cut(s) 1130
Fnu4HI GCNGC 2 cut(s) 1311, 2145
FriOI GRGCYC 1 cut(s) 1364
Fsp4HI GCNGC 2 cut(s) 1311, 2145
FspBI CTAG 2 cut(s) 1545, 1746
GlaI GCGC 4 cut(s) 429, 571, 573, 1504
GluI GCNGC 2 cut(s) 1311, 2145
HaeII RGCGCY 1 cut(s) 1506
HaeIII GGCC 1 cut(s) 1229
HapII CCGG 3 cut(s) 1402, 2036, 2171
HgaI GACGC 1 cut(s) 1255
HhaI GCGC 4 cut(s) 430, 572, 574, 1505
Hin1I GRCGYC 1 cut(s) 1503
Hin6I GCGC 4 cut(s) 428, 570, 572, 1503
HinP1I GCGC 4 cut(s) 428, 570, 572, 1503
HincII GTYRAC 1 cut(s) 2323
HindII GTYRAC 1 cut(s) 2323
HindIII AAGCTT 3 cut(s) 727, 764, 2284
HpaII CCGG 3 cut(s) 1402, 2036, 2171
HphI GGTGA 4 cut(s) 559, 872, 1039, 2290
Hpy166II GTNNAC 7 cut(s) 115, 961, 1131, 1352, 1568, 1867, 2323
Hpy188III TCNNGA 7 cut(s) 116, 928, 1017, 1402, 1665, 2008, 2192
Hpy8I GTNNAC 7 cut(s) 115, 961, 1131, 1352, 1568, 1867, 2323
HpyAV CCTTC 7 cut(s) 58, 82, 456, 1594, 1643, 1941, 2206
HpyCH4III ACNGT 4 cut(s) 1281, 1553, 1715, 2327
HpyCH4IV ACGT 5 cut(s) 416, 1007, 1212, 2196, 2334
HpyF10VI GCNNNNNNNGC 5 cut(s) 434, 1255, 1268, 1773, 2153
HpyF3I CTNAG 5 cut(s) 56, 76, 180, 1482, 2024
HpySE526I ACGT 5 cut(s) 416, 1007, 1212, 2196, 2334
Hsp92I GRCGYC 1 cut(s) 1503
HspAI GCGC 4 cut(s) 428, 570, 572, 1503
KasI GGCGCC 1 cut(s) 1502
Kpn2I TCCGGA 1 cut(s) 1401
KspI CCGCGG 1 cut(s) 2280
Kzo9I GATC 7 cut(s) 183, 283, 511, 1111, 1398, 1756, 2295
Lsp1109I GCAGC 2 cut(s) 1297, 2156
LweI GCATC 6 cut(s) 256, 582, 776, 1281, 1972, 2374
MabI ACCWGGT 1 cut(s) 2064
MaeI CTAG 2 cut(s) 1545, 1746
MaeII ACGT 5 cut(s) 416, 1007, 1212, 2196, 2334
MaeIII GTNAC 9 cut(s) 126, 715, 860, 991, 1321, 1426, 2192, 2327, 2335
MalI GATC 7 cut(s) 185, 285, 513, 1113, 1400, 1758, 2297
MboI GATC 7 cut(s) 183, 283, 511, 1111, 1398, 1756, 2295
MflI RGATCY 1 cut(s) 1756
MhlI GDGCHC 1 cut(s) 1364
Mly113I GGCGCC 1 cut(s) 1503
MlyI GAGTC 4 cut(s) 974, 1039, 1318, 2016
MmeI TCCRAC 2 cut(s) 596, 2038
Mph1103I ATGCAT 1 cut(s) 791
MroI TCCGGA 1 cut(s) 1401
MroXI GAANNNNTTC 1 cut(s) 306
MslI CAYNNNNRTG 4 cut(s) 683, 1089, 1563, 1975
MspA1I CMGCKG 3 cut(s) 1058, 1898, 2279
MspI CCGG 3 cut(s) 1402, 2036, 2171
MspR9I CCNGG 5 cut(s) 521, 1824, 2037, 2066, 2171
Mva1269I GAATGC 2 cut(s) 942, 2255
MvaI CCWGG 3 cut(s) 521, 1824, 2066
MvnI CGCG 2 cut(s) 572, 2279
MwoI GCNNNNNNNGC 5 cut(s) 434, 1255, 1268, 1773, 2153
NarI GGCGCC 1 cut(s) 1503
NciI CCSGG 2 cut(s) 2037, 2171
NcoI CCATGG 2 cut(s) 321, 1023
NdeII GATC 7 cut(s) 183, 283, 511, 1111, 1398, 1756, 2295
NlaIV GGNNCC 3 cut(s) 1021, 1504, 1627
NmeAIII GCCGAG 2 cut(s) 1793, 1816
NmuCI GTSAC 7 cut(s) 126, 860, 991, 1321, 2192, 2327, 2335
NsiI ATGCAT 1 cut(s) 791
NspI RCATGY 3 cut(s) 868, 1262, 1453
NspV TTCGAA 1 cut(s) 1818
PaeI GCATGC 1 cut(s) 1262
PagI TCATGA 2 cut(s) 927, 2007
PauI GCGCGC 1 cut(s) 570
PciI ACATGT 1 cut(s) 1449
PctI GAATGC 2 cut(s) 942, 2255
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 7 cut(s) 516, 535, 733, 1013, 1919, 1927, 2305
PflMI CCANNNNNTGG 3 cut(s) 217, 988, 1702
PfoI TCCNGGA 1 cut(s) 2169
PkrI GCNGC 2 cut(s) 1312, 2146
PleI GAGTC 4 cut(s) 973, 1038, 1318, 2016
PluTI GGCGCC 1 cut(s) 1506
PpsI GAGTC 4 cut(s) 973, 1038, 1318, 2016
Ppu21I YACGTR 1 cut(s) 417
PscI ACATGT 1 cut(s) 1449
PshBI ATTAAT 1 cut(s) 192
Psp6I CCWGG 3 cut(s) 519, 1822, 2064
PspGI CCWGG 3 cut(s) 519, 1822, 2064
PspN4I GGNNCC 3 cut(s) 1021, 1504, 1627
PsuI RGATCY 1 cut(s) 1756
PteI GCGCGC 1 cut(s) 570
PvuII CAGCTG 1 cut(s) 1058
RsaI GTAC 6 cut(s) 223, 242, 353, 960, 1006, 1151
RsaNI GTAC 6 cut(s) 222, 241, 352, 959, 1005, 1150
RseI CAYNNNNRTG 4 cut(s) 683, 1089, 1563, 1975
SacII CCGCGG 1 cut(s) 2280
SatI GCNGC 2 cut(s) 1311, 2145
Sau3AI GATC 7 cut(s) 183, 283, 511, 1111, 1398, 1756, 2295
SchI GAGTC 4 cut(s) 974, 1039, 1318, 2016
ScrFI CCNGG 5 cut(s) 521, 1824, 2037, 2066, 2171
SduI GDGCHC 1 cut(s) 1364
SexAI ACCWGGT 1 cut(s) 2064
SfaNI GCATC 6 cut(s) 256, 582, 776, 1281, 1972, 2374
SfcI CTRYAG 1 cut(s) 1588
SfoI GGCGCC 1 cut(s) 1504
Sfr303I CCGCGG 1 cut(s) 2280
SfuI TTCGAA 1 cut(s) 1818
SgrBI CCGCGG 1 cut(s) 2280
SmiMI CAYNNNNRTG 4 cut(s) 683, 1089, 1563, 1975
SmlI CTYRAG 3 cut(s) 365, 431, 1965
SmoI CTYRAG 3 cut(s) 365, 431, 1965
SphI GCATGC 1 cut(s) 1262
SsiI CCGC 4 cut(s) 271, 1898, 2277, 2279
SspDI GGCGCC 1 cut(s) 1502
SspMI CTAG 2 cut(s) 1545, 1746
StyD4I CCNGG 5 cut(s) 519, 1822, 2035, 2064, 2169
StyI CCWWGG 2 cut(s) 321, 1023
TaaI ACNGT 4 cut(s) 1281, 1553, 1715, 2327
TaiI ACGT 5 cut(s) 419, 1010, 1215, 2199, 2337
TaqI TCGA 6 cut(s) 282, 514, 585, 1686, 1818, 2409
TatI WGTACW 1 cut(s) 1149
TfiI GAWTC 7 cut(s) 516, 535, 733, 1013, 1919, 1927, 2305
TscAI CASTG 2 cut(s) 1326, 2154
TseFI GTSAC 7 cut(s) 126, 860, 991, 1321, 2192, 2327, 2335
TseI GCWGC 2 cut(s) 1310, 2144
Tsp45I GTSAC 7 cut(s) 126, 860, 991, 1321, 2192, 2327, 2335
TspGWI ACGGA 2 cut(s) 42, 1987
TspRI CASTG 2 cut(s) 1326, 2154
Van91I CCANNNNNTGG 3 cut(s) 217, 988, 1702
VspI ATTAAT 1 cut(s) 192
XapI RAATTY 4 cut(s) 119, 213, 619, 1531
XceI RCATGY 3 cut(s) 868, 1262, 1453
XcmI CCANNNNNNNNNTGG 1 cut(s) 907
XmiI GTMKAC 1 cut(s) 1130
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 2 cut(s) 1545, 1746
Zsp2I ATGCAT 1 cut(s) 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.