RLG00000025578

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016866
Physical Location & Seq
Reverse (-)
46817564 .. 46828271
10708 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025578

Sequence Viewer

Length: 4737 bp
ATGCGTTGTCTCTTCCTTCTTCTCCATCGCTTTGTGCCCAATACACCCTTCAAGCTCAGCCCCTCATCTTCTTTCTCAGCCTATGTTTCTTCTTCTTCAATCAAATTTCCCAACTTCAACCAATCCCAACCTAAAGTTCAAATTTTTTTGTCACTCTTGCAACGATTTTCGAAAAATCTCATCTGGGTCAACTCCATTCATGCCCAGATCATCACCAATGCGCTTTTCACAGACCAAAGTTTGGTCTCAAAGCTTGTCAGGGTGTACTGTGATCTGGGTAGTTTGGTTTATGCACGCCACATGTTTGATGAATGTCCTCACCCAAAAACCATTCTTTACAATGCTATGATTTATGGGTATTTGAGAAATGAGAGGTACAATGAGGCCCTTGAGTTGTTTAGATTTATGGGTTCTTGTAATGTGGAGGTGGATAGTTACACATGTAACTATGCGCTCAAGGCGTGCATGGGGCTTTCGGATTATGATACGGGTCTGGAAGTTATGAAGAGAGCTATGGATAAAGGGGTTGAAAGCAATGGGTTCTTGGGGAGTTCAATGATAAACTTTTTGATGAAATTCGGTAATGTTGATGAGGCGCAGAGGTGTTTTGATAGGATGCCGGAAAGAGATGTTGTTTGTTGGAATTCGATGATTGGTGGCTATGTGCAAGCTGGTCAGTTCGGCAAGGCATTTGATTTGTTTCATGAAATGTGTGATTGCGGCATTAGGCCGAGTTCAGTAACTATGCTGAGCATGATTCAAGTATGCATTAAAACTGCGGATTTCAAGCTTGCGAAATGTGTTCATGGTAGCATAGTTGGACTGGGAATGAGCAATGAGGTTTCGGTGCTTACCTCGTTGGTTGATATGTATGGTAATATTGGTGACGTGCTTAGTGCATATCGGGTTTTTGAGACCATGCCCAAAAGAAATTTGGTTTCGTGGAATGTCATGATCTCAGGATGTGTTCAAAATGGTATGGTGCATGAATCGTTTGCCTTCTTTGGTAGATTAGTTGCAAGTGGTGTTAGGTTTGATTCAGGGACCATGGTGAGCCTAATTCAGGGTTGTTCCCGAACAGCTGATTTGAAAGGTGGAAAAATCCTACACTGTTGTTCATTAAGGAGGGGTTTTGACTTAAATCCCATTTTGTCCACTGCCACTGTTGATCTTTACTCTAAATGTGGTGCAGTAAAGCAGGCAACTTTTGTCTTTGACAGAATGGAAGAGAGGAATGTTATTACATGGACCGCTATGCTTGTGGGATTAACACAAAATGGGCATGCAGAAGAAGCCTTGAAGCTATTCTGTCAGATGCAAAAAGAGGGAGTTGCTGCCAATTCAGTGACACTGGTTAGTTTAGTCCATTCTTGTGCCCATTTGGGCTCTCTGAAGAAAGGAAAGAGTGTTCATGCTCACATAATCCGGCAGGGCCATTCATTTGACATAGTTAACATGACATCCTTAATTGATATGTATGCCAAGTGTGGAACAATAAAGTATGCTGAGAGGATATTTAACAATGGATCCATTTGTAGAGATGTTATCCTATGGAACTCTATGATAACAAGCTATGGTATTCATGGTCATGGACAACAAGCTCTTGGTATGTATAGAAAAATGAAGGAGGAGGGATTTGAGCCAAACGAAACCTCCTTTCTCTCTCTTCTAACTGCTTGTAGTCACTCAGGACTTCTTGAAGAGGGGATCAAGTATTTCCACAGCATGGAGAGAAACCATAACATTAGGCTGACGGAAAAACACTATGCTAGTTTTGTGGATCTTCTAAGCAGAGCAGGCCGATTTGAAGAAGCTGAGGCATTGATTGAACAAATGCCTTTTAAACCAGGAAGCTCTGTTTTTGAAGCATTGCTTAGTGGATGTCACGCTCACAAGAACATTGAACTGGGTATAAAGACGGCAGATAGATTACTTTGTATTGATTCCATGAATCCTAGTGTTTATGTCGTTGTTTCAAATATTTATGCTCAAGCAAGGAGATGGGATGATGTGAACTACATTCGAACCCTCATGAGGACAAGAGGACTAAGGAAAACTCCGGGGTGTAGCTTGATTGAAGTGGGAAACCAGGTCCACAAATTCTTCGCTGGAGACAATTCACATCCTGACTGGGCAGAGATTTATCAGCATTTAGAGAACATAAGAGTAGAAGTGGAGGCTTCTGGTTATATCCCCGATACAAGCAGTGTTCTTCGCAATGTGGATGAGCCAATGAAGATAAGGCTGCTATGGGGGCACAGCGAGAGATTAGCCATTGCATTTGGGCTCTTAAGTACACCAGCTGGGAGCTTGATTAGAATCACAAAGAATCTAAGAGTGTGTGTTGATTGTCACAATGTGACTAAACTTATATCAAAGATGATGAGGAGGGAGCTTATTGTGAGAGATGCCAATCGTTTTCATCACTTTGTGAATGGGAAGTGCTCTTGTAATGATTACTGGTCCTCAGTCTATCCAGTTGAAAGTGAAATAAGGAAGATAGTTGAACACCACATGTATAAGAACCGATTGCAGGAGGAGGCTCAGAAAAGACGTTTAAATTTTCCCTCGTATTCATCCACCCGGAAAGGACCGGATCATGCCCCACGATTCAAAGCAACCGTCAACTTCGATGGAAAAACTTTTGATAGTCCTACCTTTTACCCTACTCTTCGAGAGGCAGAGAATGCTGCAGCAGAAGAAGCATTGAACAAACGTCAAAAAGGAGGGCATAAGGCCGCAGTACTGGTGAGTAGCAAAAACCTTGGTGCATACATGTTCAAGAACCGATTGCAAGAGAAGGCTCAGAAAAGTCGTTTAAATTTACCCTCGTATTCATCCACCCGGAAAGGACCGGATCATGCCCCACAATTCAAAGCAACCGTCAACTTCAATGGAAAAACTTACAAAAGTCCTACCTTTTACCCTACGCTTAGACAGGCAGAGAATGCTGCGGCAGAAGTAGCATTGAACACAGTTGGAAAAGGAGGGCATAACCTAGCATTGGCCGCAGTACTGGCTATCCTATTCCTATTTGTACATGAATGGCTCTTTTTCTTTCTTTCTCGGGCTCTGGAAAATTGGTATCTCAGCCTCTGGAAATTGAGCTACCCCCACGAAGGCATGTATAAGAACCAATTGCAAGAGTTGGCTCAGAGAAGTTGTTTCAATTTGCCCTCTTATGCGTGCATCCGGGAAGGTCCAGATCATGCCCCTCGATTCAAAGCAACTGTCAACTTCAATGGAGATACTTTTGAAAGTCCTACATTTTGCACTACCCTTAGACAGGCAGAACATGCTGCAGCAGAGGTAGCATTGAACACACTTGCAAGAAGAGGACCTTCCAGAGCATTGGCTGCAAGAGTATTGGATGAAACTGGAGTTTACAAGAACTTGCTTCAGGAGACTGCTCACAGAGCTGGTTTGAACCTTCCTGTTTATACTACTGTTCGATCTGGACCAGGCCACGTTCCTGTTTTCTCATGTACTGTTGAGCTAGGAGGAATGAATTTTATTGGGGATCCAGCTAGGACAAAGAAACAAGCTCAGAAAAATGCTGCCATAGCTGCTTGGACATCCCTGAAAAGACTGGCTCAACGTGATTCATCTTCTCCCTCAATGGAGTCCCAAGGCATTGAAGAACAGGAACAAGTCATTATTTCTCGTATTCTTGGACATTTGCGCCCATCTGATCCTAAGAACCCTCGAAATGATCGCAGACATGGACAACATGGATACACTCCCATCTGCAGCAATTCTACCCTGCCAACGCCAAGCCTATATCCGATGCAATTCCATGACTGGGCTTATTCTAATTATTCCCCTGAACTAGCCATGTATCAATTGTGGCAGCAAGAGCCATTACTACAGCAACAAAACCGATTGTTGGCGCTTCCTGTTTTACCAGCTGTTCCATCCATTCCTCAAATATATCCATTTATGCAATCCATGCTTCACCCAGATCACCATCTCTACTTTCCATCAAGAGGGGAAGAGTCAACCTCTGTTGGACCGAAATTTACAATCTCTACCTCTGGCCCATCATTCTGCTTCTCAAACCATTTGGTTCCTAATCCGATCAGAGGCAAGTCCACAGTGACTATTCAAGAGATACAGGAAGAAAAATCAGAAGAATCAGCTGAGTACTCTCCATCTGTAGTGCCAGTTCCCTTTGGTCTTGGAAACAGTACAACTGAACTAAGAGTCCAGGAACAAGTGCGGGATGACAAACAAAAGCTTGCCGAGTTGGGAAGCAGAATTGAAAACCTTCAGCTGGAAGGGAACCAAACTGGGCAATCTGAATGGGGTAACCCAGGTAACCCAGGGACCATGGATTCTAGTTTCAAGCCGGTGGATTTTGGGTTACAAAATCCTCCAAGAATGAGTCCTCCTAGAAGTCTTAGACCACCGTCTGCTGCAGATCCTGTACTGATCAGAACTGTAAGACCGACTTCCTCACTAGGATCTAGACTGTCTAGACCACAGAATTTGCCAACCAGGTTTCCTGCTCCACCAAGAATGAGAGCTGGAATCCCTTCATATTCAGCCACACCCACGCTTCAAAGAATGGAATTTGGCAGACCAAGACCTAGCTTCATGGCACCAGCTGTTCGAATTAGGTCAGTTGTACCAGTTTGTTCAGCTCCACCAGCAAGAAGCATGCAAAGTTCCAGCCAGGAGACTGTGATACCCAACATGGAGAAGAAAGATACCAGTGACATGTCAAAATCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

1579

Amino Acids

176.76

Weight (kDa)

9.08

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 108 - 155 2.1e-09 PPR repeat family
PPR_2 PF13041 209 - 257 9.8e-14 PPR repeat family
PPR PF01535 212 - 242 1.2e-08 PPR repeat
PPR_2 PF13041 311 - 359 5.6e-07 PPR repeat family
PPR PF01535 313 - 343 6.4e-06 PPR repeat
PPR_2 PF13041 411 - 460 3.5e-11 PPR repeat family
PPR PF01535 414 - 444 6.1e-07 PPR repeat
PPR_2 PF13041 513 - 561 2.7e-10 PPR repeat family
PPR PF01535 517 - 546 8.2e-06 PPR repeat
E_motif PF20431 631 - 693 1.8e-19 E motif
Eplus_motif PF20430 698 - 721 2.7e-06 E+ motif
DYW_deaminase PF14432 729 - 821 6.9e-41 DYW family of nucleic acid deaminases
dsrm PF00035 840 - 905 9.6e-13 Double-stranded RNA binding motif
dsrm PF00035 928 - 991 1.1e-12 Double-stranded RNA binding motif
dsrm PF00035 1043 - 1109 6.9e-14 Double-stranded RNA binding motif
dsrm PF00035 1129 - 1193 7.2e-15 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016866)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04050
malus_domestica MD04G1216800.v1.1
prunus_persica Prupe.6G336500_v2.0.a1
pyrus_communis pycom04g19280
rosa_chinensis RchiOBHm_Chr3g0452611
rosa_laevigata RLG00000025578
rosa_multiflora Rmu_sc0011540.1_g000002 Rmu_sc0034042.1_g000001
rosa_roxburghii Rroxscaffold_6G00429650
rosa_rugosa Rorug02G0641700
rosa_samantha Rh3AG044200 Rh3BG046500 Rh3DG045500
rosa_wichuraiana Rw3G003440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4600
AccB7I CCANNNNNTGG 2 cut(s) 241, 1726
AciI CCGC 7 cut(s) 720, 779, 1251, 2739, 2954, 3009, 4219
AcoI YGGCCR 1 cut(s) 3006
AcuI CTGAAG 3 cut(s) 1412, 3383, 4253
AdeI CACNNNGTG 2 cut(s) 2359, 2431
AflII CTTAAG 1 cut(s) 2290
AflIII ACRYGT 5 cut(s) 300, 440, 2514, 2775, 4719
AjiI CACGTC 1 cut(s) 889
AjnI CCWGG 8 cut(s) 1846, 2088, 3460, 4206, 4312, 4321, 4496, 4674
AjuI GAANNNNNNNTTGG 6 cut(s) 32, 64, 120, 152, 209, 241
AloI GAACNNNNNNTCC 4 cut(s) 1392, 1424, 4188, 4220
Alw21I GWGCWC 1 cut(s) 2447
Alw26I GTCTC 6 cut(s) 14, 250, 908, 2106, 3398, 4673
AlwNI CAGNNNCTG 2 cut(s) 3096, 4424
Ama87I CYCGRG 1 cut(s) 3066
AoxI GGCC 8 cut(s) 384, 728, 1432, 1798, 2736, 3006, 3463, 4036
ArsI GACNNNNNNTTYG 6 cut(s) 2607, 2639, 2868, 2900, 3216, 3248
Asp700I GAANNNNTTC 3 cut(s) 1304, 4266, 4534
AspLEI GCGC 5 cut(s) 223, 454, 598, 3684, 3892
AsuC2I CCSGG 4 cut(s) 2061, 2584, 2845, 3194
AsuHPI GGTGA 7 cut(s) 205, 311, 896, 1063, 2761, 3947, 3956
AsuII TTCGAA 3 cut(s) 170, 2023, 4612
AvaI CYCGRG 1 cut(s) 3066
BaeGI GKGCMC 3 cut(s) 39, 1378, 2259
BamHI GGATCC 2 cut(s) 1526, 3520
BanI GGYRCC 1 cut(s) 4600
BanII GRGCYC 3 cut(s) 1388, 2289, 3073
Bbv12I GWGCWC 1 cut(s) 2447
BbvCI CCTCAGC 1 cut(s) 1815
BceAI ACGGC 1 cut(s) 1935
BciT130I CCWGG 8 cut(s) 1848, 2090, 3462, 4208, 4314, 4323, 4498, 4676
BciVI GTATCC 1 cut(s) 3728
BclI TGATCA 1 cut(s) 4431
BcnI CCSGG 4 cut(s) 2061, 2584, 2845, 3194
BcoDI GTCTC 6 cut(s) 14, 250, 908, 2106, 3398, 4673
BfmI CTRYAG 6 cut(s) 2691, 3300, 3748, 3866, 4155, 4416
BfoI RGCGCY 1 cut(s) 3893
BfrI CTTAAG 1 cut(s) 2290
BfuI GTATCC 1 cut(s) 3728
BlpI GCTNAGC 2 cut(s) 56, 749
BmcAI AGTACT 3 cut(s) 2745, 3015, 4145
BmeT110I CYCGRG 1 cut(s) 3066
BmgBI CACGTC 1 cut(s) 889
BmiI GGNNCC 7 cut(s) 1045, 1528, 3522, 4068, 4283, 4327, 4602
BmrI ACTGGG 5 cut(s) 833, 1916, 2140, 3811, 4299
BmsI GCATC 5 cut(s) 606, 1305, 2398, 3198, 3777
BmuI ACTGGG 5 cut(s) 833, 1916, 2140, 3811, 4299
BoxI GACNNNNGTC 1 cut(s) 4408
BplI GAGNNNNNCTC 2 cut(s) 3986, 4018
BpmI CTGGAG 2 cut(s) 2130, 3399
Bpu10I CCTNAGC 1 cut(s) 1815
Bpu1102I GCTNAGC 2 cut(s) 56, 749
Bpu14I TTCGAA 3 cut(s) 170, 2023, 4612
BpuEI CTTGAG 3 cut(s) 410, 440, 1974
BpuMI CCSGG 4 cut(s) 2061, 2584, 2845, 3194
BsaBI GATNNNNATC 3 cut(s) 2318, 2412, 3966
BsaI GGTCTC 2 cut(s) 250, 908
BsaJI CCNNGG 8 cut(s) 1047, 2060, 2764, 3628, 4312, 4321, 4322, 4329
BsaWI WCCGGW 2 cut(s) 2593, 2854
BsaXI ACNNNNNCTCC 2 cut(s) 1637, 1667
Bse118I RCCGGY 1 cut(s) 4348
Bse3DI GCAATG 5 cut(s) 541, 841, 1868, 2224, 2274
Bse8I GATNNNNATC 3 cut(s) 2318, 2412, 3966
BseBI CCWGG 8 cut(s) 1848, 2090, 3462, 4208, 4314, 4323, 4498, 4676
BseDI CCNNGG 8 cut(s) 1047, 2060, 2764, 3628, 4312, 4321, 4322, 4329
BseJI GATNNNNATC 3 cut(s) 2318, 2412, 3966
BseMI GCAATG 5 cut(s) 541, 841, 1868, 2224, 2274
BseRI GAGGAG 3 cut(s) 1643, 2401, 2552
BseSI GKGCMC 3 cut(s) 39, 1378, 2259
BseYI CCCAGC 1 cut(s) 2303
BsgI GTGCAG 1 cut(s) 1209
BshFI GGCC 8 cut(s) 386, 730, 1434, 1800, 2738, 3008, 3465, 4038
BshNI GGYRCC 1 cut(s) 4600
BsiHKAI GWGCWC 1 cut(s) 2447
BsiHKCI CYCGRG 1 cut(s) 3066
BsiSI CCGG 9 cut(s) 620, 1426, 2060, 2584, 2594, 2845, 2855, 3193, 4349
BslFI GGGAC 3 cut(s) 1057, 3610, 4339
BsmAI GTCTC 6 cut(s) 14, 250, 908, 2106, 3398, 4673
BsmFI GGGAC 3 cut(s) 1057, 3610, 4339
BsmI GAATGC 2 cut(s) 2692, 2953
BsnI GGCC 8 cut(s) 386, 730, 1434, 1800, 2738, 3008, 3465, 4038
Bso31I GGTCTC 2 cut(s) 250, 908
BsoBI CYCGRG 1 cut(s) 3066
Bsp119I TTCGAA 3 cut(s) 170, 2023, 4612
Bsp1286I GDGCHC 7 cut(s) 39, 1378, 1388, 2259, 2289, 2447, 3073
Bsp1407I TGTACA 1 cut(s) 3037
Bsp1720I GCTNAGC 2 cut(s) 56, 749
Bsp19I CCATGG 2 cut(s) 1047, 4329
BspACI CCGC 7 cut(s) 720, 779, 1251, 2739, 2954, 3009, 4219
BspANI GGCC 8 cut(s) 386, 730, 1434, 1800, 2738, 3008, 3465, 4038
BspHI TCATGA 3 cut(s) 703, 951, 2031
BspLI GGNNCC 7 cut(s) 1045, 1528, 3522, 4068, 4283, 4327, 4602
BspMAI CTGCAG 4 cut(s) 2695, 3304, 3752, 4420
BspT104I TTCGAA 3 cut(s) 170, 2023, 4612
BspT107I GGYRCC 1 cut(s) 4600
BspTI CTTAAG 1 cut(s) 2290
BspTNI GGTCTC 2 cut(s) 250, 908
BsrDI GCAATG 5 cut(s) 541, 841, 1868, 2224, 2274
BsrFI RCCGGY 1 cut(s) 4348
BsrGI TGTACA 1 cut(s) 3037
BssAI RCCGGY 1 cut(s) 4348
BssECI CCNNGG 8 cut(s) 1047, 2060, 2764, 3628, 4312, 4321, 4322, 4329
BssT1I CCWWGG 4 cut(s) 1047, 2764, 3628, 4329
Bst2UI CCWGG 8 cut(s) 1848, 2090, 3462, 4208, 4314, 4323, 4498, 4676
Bst6I CTCTTC 8 cut(s) 17, 500, 1221, 1671, 1695, 2676, 3328, 3987
BstAFI CTTAAG 1 cut(s) 2290
BstAPI GCANNNNNTGC 5 cut(s) 2687, 2948, 3296, 3356, 3949
BstAUI TGTACA 1 cut(s) 3037
BstBI TTCGAA 3 cut(s) 170, 2023, 4612
BstDSI CCRYGG 2 cut(s) 1047, 4329
BstEII GGTNACC 2 cut(s) 4307, 4316
BstENI CCTNNNNNAGG 2 cut(s) 1061, 3284
BstH2I RGCGCY 1 cut(s) 3893
BstHHI GCGC 5 cut(s) 223, 454, 598, 3684, 3892
BstMAI GTCTC 6 cut(s) 14, 250, 908, 2106, 3398, 4673
BstNI CCWGG 8 cut(s) 1848, 2090, 3462, 4208, 4314, 4323, 4498, 4676
BstNSI RCATGY 9 cut(s) 304, 444, 1286, 2518, 2779, 3127, 3299, 4663, 4723
BstPAI GACNNNNGTC 1 cut(s) 4408
BstPI GGTNACC 2 cut(s) 4307, 4316
BstSFI CTRYAG 6 cut(s) 2691, 3300, 3748, 3866, 4155, 4416
BstSLI GKGCMC 3 cut(s) 39, 1378, 2259
BstX2I RGATCY 5 cut(s) 1526, 1780, 3520, 4420, 4463
BstXI CCANNNNNNTGG 1 cut(s) 3352
BstYI RGATCY 5 cut(s) 1526, 1780, 3520, 4420, 4463
BsuI GTATCC 1 cut(s) 3728
BsuRI GGCC 8 cut(s) 386, 730, 1434, 1800, 2738, 3008, 3465, 4038
BtgI CCRYGG 2 cut(s) 1047, 4329
BtgZI GCGATG 1 cut(s) 11
BtrI CACGTC 1 cut(s) 889
BtsI GCAGTG 2 cut(s) 1155, 2212
BtsIMutI CAGTG 8 cut(s) 1108, 1155, 1161, 1349, 1350, 2212, 4101, 4720
CaiI CAGNNNCTG 2 cut(s) 3096, 4424
CciI TCATGA 3 cut(s) 703, 951, 2031
CfoI GCGC 5 cut(s) 223, 454, 598, 3684, 3892
Cfr10I RCCGGY 1 cut(s) 4348
CsiI ACCWGGT 2 cut(s) 2088, 4496
CspCI CAANNNNNGTGG 2 cut(s) 4470, 4505
DraI TTTAAA 3 cut(s) 1843, 2559, 2820
DraIII CACNNNGTG 2 cut(s) 2359, 2431
EaeI YGGCCR 1 cut(s) 3006
Eam1104I CTCTTC 8 cut(s) 17, 500, 1221, 1671, 1695, 2676, 3328, 3987
EarI CTCTTC 8 cut(s) 17, 500, 1221, 1671, 1695, 2676, 3328, 3987
Eco130I CCWWGG 4 cut(s) 1047, 2764, 3628, 4329
Eco24I GRGCYC 3 cut(s) 1388, 2289, 3073
Eco31I GGTCTC 2 cut(s) 250, 908
Eco57I CTGAAG 3 cut(s) 1412, 3383, 4253
Eco88I CYCGRG 1 cut(s) 3066
Eco91I GGTNACC 2 cut(s) 4307, 4316
EcoNI CCTNNNNNAGG 2 cut(s) 1061, 3284
EcoO109I RGGNCCY 2 cut(s) 385, 3338
EcoO65I GGTNACC 2 cut(s) 4307, 4316
EcoRI GAATTC 1 cut(s) 643
EcoRII CCWGG 8 cut(s) 1846, 2088, 3460, 4206, 4312, 4321, 4496, 4674
EcoT14I CCWWGG 4 cut(s) 1047, 2764, 3628, 4329
EcoT22I ATGCAT 1 cut(s) 770
EcoT38I GRGCYC 3 cut(s) 1388, 2289, 3073
ErhI CCWWGG 4 cut(s) 1047, 2764, 3628, 4329
FalI AAGNNNNNCTT 6 cut(s) 1857, 1889, 3325, 3357, 4435, 4467
FaqI GGGAC 3 cut(s) 1057, 3610, 4339
FauI CCCGC 1 cut(s) 4212
FbaI TGATCA 1 cut(s) 4431
FriOI GRGCYC 3 cut(s) 1388, 2289, 3073
GlaI GCGC 5 cut(s) 222, 453, 597, 3683, 3891
GsaI CCCAGC 1 cut(s) 2307
GsuI CTGGAG 2 cut(s) 2130, 3399
HaeII RGCGCY 1 cut(s) 3893
HaeIII GGCC 8 cut(s) 386, 730, 1434, 1800, 2738, 3008, 3465, 4038
HapII CCGG 9 cut(s) 620, 1426, 2060, 2584, 2594, 2845, 2855, 3193, 4349
HhaI GCGC 5 cut(s) 223, 454, 598, 3684, 3892
Hin6I GCGC 5 cut(s) 221, 452, 596, 3682, 3890
HinP1I GCGC 5 cut(s) 221, 452, 596, 3682, 3890
HincII GTYRAC 6 cut(s) 190, 1453, 2626, 2887, 3235, 3999
HindII GTYRAC 6 cut(s) 190, 1453, 2626, 2887, 3235, 3999
HindIII AAGCTT 3 cut(s) 251, 788, 4235
HpaI GTTAAC 1 cut(s) 1453
HpaII CCGG 9 cut(s) 620, 1426, 2060, 2584, 2594, 2845, 2855, 3193, 4349
HphI GGTGA 7 cut(s) 205, 311, 896, 1063, 2761, 3947, 3956
HpyCH4IV ACGT 5 cut(s) 888, 2554, 2716, 3468, 3598
HpySE526I ACGT 5 cut(s) 888, 2554, 2716, 3468, 3598
HspAI GCGC 5 cut(s) 221, 452, 596, 3682, 3890
Ksp22I TGATCA 1 cut(s) 4431
KspAI GTTAAC 1 cut(s) 1453
LmnI GCTCC 4 cut(s) 2307, 2392, 4513, 4648
LweI GCATC 5 cut(s) 606, 1305, 2398, 3198, 3777
MabI ACCWGGT 2 cut(s) 2088, 4496
MaeII ACGT 5 cut(s) 888, 2554, 2716, 3468, 3598
MfeI CAATTG 2 cut(s) 3137, 3842
MflI RGATCY 5 cut(s) 1526, 1780, 3520, 4420, 4463
MhlI GDGCHC 7 cut(s) 39, 1378, 1388, 2259, 2289, 2447, 3073
MlyI GAGTC 4 cut(s) 3632, 4004, 4212, 4393
MmeI TCCRAC 4 cut(s) 620, 799, 2959, 3988
Mph1103I ATGCAT 1 cut(s) 770
MroXI GAANNNNTTC 3 cut(s) 1304, 4266, 4534
MslI CAYNNNNRTG 2 cut(s) 1371, 1587
MspA1I CMGCKG 6 cut(s) 1082, 2303, 3908, 4139, 4273, 4607
MspCI CTTAAG 1 cut(s) 2290
MspI CCGG 9 cut(s) 620, 1426, 2060, 2584, 2594, 2845, 2855, 3193, 4349
MunI CAATTG 2 cut(s) 3137, 3842
Mva1269I GAATGC 2 cut(s) 2692, 2953
MvaI CCWGG 8 cut(s) 1848, 2090, 3462, 4208, 4314, 4323, 4498, 4676
NciI CCSGG 4 cut(s) 2061, 2584, 2845, 3194
NcoI CCATGG 2 cut(s) 1047, 4329
NlaIV GGNNCC 7 cut(s) 1045, 1528, 3522, 4068, 4283, 4327, 4602
NmeAIII GCCGAG 2 cut(s) 756, 4267
NmuCI GTSAC 9 cut(s) 150, 884, 1345, 1682, 1883, 2351, 2359, 4096, 4715
NsiI ATGCAT 1 cut(s) 770
NspI RCATGY 9 cut(s) 304, 444, 1286, 2518, 2779, 3127, 3299, 4663, 4723
NspV TTCGAA 3 cut(s) 170, 2023, 4612
PaeI GCATGC 2 cut(s) 1286, 4663
PagI TCATGA 3 cut(s) 703, 951, 2031
PasI CCCWGGG 1 cut(s) 4322
PciI ACATGT 5 cut(s) 300, 440, 2514, 2775, 4719
PctI GAATGC 2 cut(s) 2692, 2953
PdmI GAANNNNTTC 3 cut(s) 1304, 4266, 4534
PflMI CCANNNNNTGG 2 cut(s) 241, 1726
PfoI TCCNGGA 2 cut(s) 3192, 4206
PleI GAGTC 4 cut(s) 3631, 4003, 4211, 4392
PpsI GAGTC 4 cut(s) 3631, 4003, 4211, 4392
PpuMI RGGWCCY 1 cut(s) 3338
PscI ACATGT 5 cut(s) 300, 440, 2514, 2775, 4719
PshAI GACNNNNGTC 1 cut(s) 4408
Psp5II RGGWCCY 1 cut(s) 3338
Psp6I CCWGG 8 cut(s) 1846, 2088, 3460, 4206, 4312, 4321, 4496, 4674
PspEI GGTNACC 2 cut(s) 4307, 4316
PspFI CCCAGC 1 cut(s) 2303
PspGI CCWGG 8 cut(s) 1846, 2088, 3460, 4206, 4312, 4321, 4496, 4674
PspN4I GGNNCC 7 cut(s) 1045, 1528, 3522, 4068, 4283, 4327, 4602
PspPPI RGGWCCY 1 cut(s) 3338
PsrI GAACNNNNNNTAC 2 cut(s) 4149, 4181
PstI CTGCAG 4 cut(s) 2695, 3304, 3752, 4420
PstNI CAGNNNCTG 2 cut(s) 3096, 4424
PsuI RGATCY 5 cut(s) 1526, 1780, 3520, 4420, 4463
PvuII CAGCTG 6 cut(s) 1082, 2303, 3908, 4139, 4273, 4607
RseI CAYNNNNRTG 2 cut(s) 1371, 1587
ScaI AGTACT 3 cut(s) 2745, 3015, 4145
SchI GAGTC 4 cut(s) 3632, 4004, 4212, 4393
SduI GDGCHC 7 cut(s) 39, 1378, 1388, 2259, 2289, 2447, 3073
SexAI ACCWGGT 2 cut(s) 2088, 4496
SfaNI GCATC 5 cut(s) 606, 1305, 2398, 3198, 3777
SfcI CTRYAG 6 cut(s) 2691, 3300, 3748, 3866, 4155, 4416
SfuI TTCGAA 3 cut(s) 170, 2023, 4612
SmiMI CAYNNNNRTG 2 cut(s) 1371, 1587
SmlI CTYRAG 4 cut(s) 389, 455, 1989, 2290
SmoI CTYRAG 4 cut(s) 389, 455, 1989, 2290
SphI GCATGC 2 cut(s) 1286, 4663
SsiI CCGC 7 cut(s) 720, 779, 1251, 2739, 2954, 3009, 4219
SspI AATATT 2 cut(s) 880, 1981
StyI CCWWGG 4 cut(s) 1047, 2764, 3628, 4329
TaiI ACGT 5 cut(s) 891, 2557, 2719, 3471, 3601
TaqI TCGA 9 cut(s) 170, 647, 2023, 2631, 2674, 3217, 3451, 3706, 4612
TaqII GACCGA 2 cut(s) 4027, 4462
TatI WGTACW 9 cut(s) 264, 2294, 2743, 3013, 3037, 3485, 4143, 4187, 4426
TauI GCSGC 4 cut(s) 723, 2741, 2957, 3011
TscAI CASTG 8 cut(s) 1115, 1162, 1168, 1350, 1356, 2212, 4101, 4720
TseFI GTSAC 9 cut(s) 150, 884, 1345, 1682, 1883, 2351, 2359, 4096, 4715
Tsp45I GTSAC 9 cut(s) 150, 884, 1345, 1682, 1883, 2351, 2359, 4096, 4715
TspGWI ACGGA 1 cut(s) 1769
TspRI CASTG 8 cut(s) 1115, 1162, 1168, 1350, 1356, 2212, 4101, 4720
Van91I CCANNNNNTGG 2 cut(s) 241, 1726
Vha464I CTTAAG 1 cut(s) 2290
XagI CCTNNNNNAGG 2 cut(s) 1061, 3284
XbaI TCTAGA 2 cut(s) 4466, 4475
XceI RCATGY 9 cut(s) 304, 444, 1286, 2518, 2779, 3127, 3299, 4663, 4723
XcmI CCANNNNNNNNNTGG 1 cut(s) 931
XmnI GAANNNNTTC 3 cut(s) 1304, 4266, 4534
ZrmI AGTACT 3 cut(s) 2745, 3015, 4145
Zsp2I ATGCAT 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.