FvH4_6g04220

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013966
Physical Location & Seq
Reverse (-)
2283264 .. 2284294
1031 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04220.t1

Sequence Viewer

Length: 405 bp
ATGTTCGACTTCCGCTCCCTCACCTTCCCCCACTGCTTCGACGACGAGCTCACGCGCCTCCGCTACAACGCCGCCTACTTCCGCATGAACTACGCCATCGTCGTCCTCCTAATCCTCTTCCTCAGCCTCCTCTGGCACCTGATCTCGCTCATCGTCCTCATCGTGATGCTCGCCGCCTGGCTCTTCCTCTACTTCCTCCCAGACGAGCCGCTCGTCCTCTTCGGCAGCACTGTCGTGAAGATTGTGCTTCTCTTTCTGACGTCGGCCACGATGAACATCCTGGTGGCTCTGTCGATCGCAGTGGTGGTGGTGGTGGTGCACGTGATGCTGAGGAAGACAAAGGATTTGCACGTCAATAAGGAGAACACTGGATTGATGACCGGCCCGGCCGGATCTTCAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

135

Amino Acids

15.15

Weight (kDa)

7.94

Isoelectric Point (pI)

32.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 1 - 119 5.2e-29 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013966)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17700
fragaria_vesca FvH4_1g19140 FvH4_6g04220 FvH4_6g33130
malus_domestica MD14G1066400.v1.1 MD17G1045700.v1.1 MD17G1175300.v1.1
prunus_persica Prupe.3G051300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0142821
rosa_laevigata RLG00000019988
rosa_multiflora Rmu_sc0002358.1_g000017
rosa_roxburghii Rroxscaffold_2G00102290 Rroxscaffold_2G00103340
rosa_rugosa Rorug02G0375500
rosa_samantha Rh2AG428600 Rh2CG414200
rosa_wichuraiana Rw2G035050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 263
AccB1I GGYRCC 1 cut(s) 135
AccBSI CCGCTC 2 cut(s) 15, 211
AccII CGCG 1 cut(s) 55
AciI CCGC 6 cut(s) 13, 61, 72, 82, 174, 209
AclWI GGATC 1 cut(s) 400
AcoI YGGCCR 2 cut(s) 264, 387
AcuI CTGAAG 1 cut(s) 381
AcvI CACGTG 1 cut(s) 322
AcyI GRCGYC 1 cut(s) 260
AjiI CACGTC 1 cut(s) 352
AjnI CCWGG 2 cut(s) 176, 279
AleI CACNNNNGTG 1 cut(s) 233
AluBI AGCT 2 cut(s) 49, 401
AluI AGCT 2 cut(s) 49, 401
Alw21I GWGCWC 2 cut(s) 51, 321
Alw44I GTGCAC 1 cut(s) 317
AlwI GGATC 1 cut(s) 400
AoxI GGCC 3 cut(s) 264, 382, 387
ApaLI GTGCAC 1 cut(s) 317
ApeKI GCWGC 1 cut(s) 225
ArsI GACNNNNNNTTYG 2 cut(s) 328, 360
AspLEI GCGC 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 383
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 1 cut(s) 13
BaeGI GKGCMC 1 cut(s) 321
BanI GGYRCC 1 cut(s) 135
BanII GRGCYC 1 cut(s) 51
BbrPI CACGTG 1 cut(s) 322
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 2 cut(s) 51, 321
BbvCI CCTCAGC 2 cut(s) 122, 329
BbvI GCAGC 1 cut(s) 237
BccI CCATC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 214, 248
BciT130I CCWGG 2 cut(s) 178, 281
BcnI CCSGG 1 cut(s) 386
BisI GCNGC 4 cut(s) 72, 174, 209, 226
BlsI GCNGC 4 cut(s) 73, 175, 210, 227
Bme1390I CCNGG 3 cut(s) 178, 281, 386
BmgBI CACGTC 1 cut(s) 352
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 3 cut(s) 178, 281, 386
BmsI GCATC 2 cut(s) 156, 315
BpiI GAAGAC 1 cut(s) 341
Bpu10I CCTNAGC 2 cut(s) 122, 329
BpuMI CCSGG 1 cut(s) 386
BsaAI YACGTR 1 cut(s) 322
BsaBI GATNNNNATC 1 cut(s) 275
BsaHI GRCGYC 1 cut(s) 260
BsaXI ACNNNNNCTCC 1 cut(s) 29
Bse118I RCCGGY 1 cut(s) 380
Bse1I ACTGG 1 cut(s) 373
Bse8I GATNNNNATC 1 cut(s) 275
BseBI CCWGG 2 cut(s) 178, 281
BseGI GGATG 1 cut(s) 276
BseJI GATNNNNATC 1 cut(s) 275
BseMII CTCAG 2 cut(s) 136, 320
BseNI ACTGG 1 cut(s) 373
BseRI GAGGAG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 321
BseX3I CGGCCG 1 cut(s) 387
BseXI GCAGC 1 cut(s) 237
Bsh1236I CGCG 1 cut(s) 55
Bsh1285I CGRYCG 2 cut(s) 297, 390
BshFI GGCC 3 cut(s) 266, 384, 389
BshNI GGYRCC 1 cut(s) 135
BsiEI CGRYCG 2 cut(s) 297, 390
BsiHKAI GWGCWC 2 cut(s) 51, 321
BsiSI CCGG 3 cut(s) 381, 386, 390
BsnI GGCC 3 cut(s) 266, 384, 389
Bsp1286I GDGCHC 2 cut(s) 51, 321
Bsp143I GATC 3 cut(s) 141, 294, 392
BspACI CCGC 6 cut(s) 13, 61, 72, 82, 174, 209
BspANI GGCC 3 cut(s) 266, 384, 389
BspCNI CTCAG 2 cut(s) 135, 321
BspFNI CGCG 1 cut(s) 55
BspLI GGNNCC 1 cut(s) 137
BspPI GGATC 1 cut(s) 400
BspQI GCTCTTC 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 135
BsrBI CCGCTC 2 cut(s) 15, 211
BsrFI RCCGGY 1 cut(s) 380
BsrI ACTGG 1 cut(s) 373
BssAI RCCGGY 1 cut(s) 380
BssMI GATC 3 cut(s) 141, 294, 392
BssNI GRCGYC 1 cut(s) 260
Bst2UI CCWGG 2 cut(s) 178, 281
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 3 cut(s) 122, 188, 224
BstACI GRCGYC 1 cut(s) 260
BstAPI GCANNNNNTGC 1 cut(s) 325
BstBAI YACGTR 1 cut(s) 322
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 3 cut(s) 122, 329, 402
BstF5I GGATG 1 cut(s) 276
BstFNI CGCG 1 cut(s) 55
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 3 cut(s) 144, 297, 395
BstMBI GATC 3 cut(s) 141, 294, 392
BstMCI CGRYCG 2 cut(s) 297, 390
BstMWI GCNNNNNNNGC 1 cut(s) 325
BstNI CCWGG 2 cut(s) 178, 281
BstSCI CCNGG 3 cut(s) 176, 279, 384
BstSLI GKGCMC 1 cut(s) 321
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 237
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 1 cut(s) 392
BstYI RGATCY 1 cut(s) 392
BstZI CGGCCG 1 cut(s) 387
BsuRI GGCC 3 cut(s) 266, 384, 389
BtrI CACGTC 1 cut(s) 352
BtsCI GGATG 1 cut(s) 276
BtsI GCAGTG 2 cut(s) 31, 306
BtsIMutI CAGTG 4 cut(s) 31, 228, 306, 366
Cac8I GCNNGC 1 cut(s) 171
CfoI GCGC 1 cut(s) 57
Cfr10I RCCGGY 1 cut(s) 380
Cfr13I GGNCC 1 cut(s) 383
CviAII CATG 1 cut(s) 85
CviJI RGCY 9 cut(s) 49, 126, 181, 208, 266, 287, 384, 389, 401
CviKI_1 RGCY 9 cut(s) 49, 126, 181, 208, 266, 287, 384, 389, 401
DdeI CTNAG 3 cut(s) 122, 329, 402
DpnI GATC 3 cut(s) 143, 296, 394
DpnII GATC 3 cut(s) 141, 294, 392
EaeI YGGCCR 2 cut(s) 264, 387
EagI CGGCCG 1 cut(s) 387
Eam1104I CTCTTC 3 cut(s) 122, 188, 224
EarI CTCTTC 3 cut(s) 122, 188, 224
Ecl136II GAGCTC 1 cut(s) 49
EclXI CGGCCG 1 cut(s) 387
Eco24I GRGCYC 1 cut(s) 51
Eco52I CGGCCG 1 cut(s) 387
Eco53kI GAGCTC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 381
Eco72I CACGTG 1 cut(s) 322
EcoICRI GAGCTC 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 176, 279
EcoT38I GRGCYC 1 cut(s) 51
FaeI CATG 1 cut(s) 88
FaiI YATR 1 cut(s) 86
FatI CATG 1 cut(s) 84
Fnu4HI GCNGC 4 cut(s) 72, 174, 209, 226
FokI GGATG 1 cut(s) 263
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 4 cut(s) 72, 174, 209, 226
GlaI GCGC 1 cut(s) 56
GluI GCNGC 4 cut(s) 72, 174, 209, 226
HaeIII GGCC 3 cut(s) 266, 384, 389
HapII CCGG 3 cut(s) 381, 386, 390
HhaI GCGC 1 cut(s) 57
Hin1I GRCGYC 1 cut(s) 260
Hin1II CATG 1 cut(s) 88
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HpaII CCGG 3 cut(s) 381, 386, 390
HphI GGTGA 1 cut(s) 13
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 1 cut(s) 258
Hpy188III TCNNGA 2 cut(s) 163, 235
Hpy8I GTNNAC 1 cut(s) 319
Hpy99I CGWCG 4 cut(s) 44, 47, 104, 265
HpyAV CCTTC 1 cut(s) 34
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 3 cut(s) 260, 321, 351
HpyCH4V TGCA 2 cut(s) 319, 349
HpyF10VI GCNNNNNNNGC 1 cut(s) 325
HpyF3I CTNAG 3 cut(s) 122, 329, 402
HpySE526I ACGT 3 cut(s) 260, 321, 351
Hsp92I GRCGYC 1 cut(s) 260
Hsp92II CATG 1 cut(s) 88
HspAI GCGC 1 cut(s) 55
Kzo9I GATC 3 cut(s) 141, 294, 392
LguI GCTCTTC 1 cut(s) 188
LmnI GCTCC 1 cut(s) 20
Lsp1109I GCAGC 1 cut(s) 237
LweI GCATC 2 cut(s) 156, 315
MaeII ACGT 3 cut(s) 260, 321, 351
MalI GATC 3 cut(s) 143, 296, 394
MbiI CCGCTC 2 cut(s) 15, 211
MboI GATC 3 cut(s) 141, 294, 392
MboII GAAGA 6 cut(s) 109, 175, 211, 250, 346, 387
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 2 cut(s) 51, 321
MslI CAYNNNNRTG 3 cut(s) 164, 233, 281
MspI CCGG 3 cut(s) 381, 386, 390
MspR9I CCNGG 3 cut(s) 178, 281, 386
MvaI CCWGG 2 cut(s) 178, 281
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 1 cut(s) 325
NciI CCSGG 1 cut(s) 386
NdeII GATC 3 cut(s) 141, 294, 392
NlaIII CATG 1 cut(s) 88
NlaIV GGNNCC 1 cut(s) 137
OliI CACNNNNGTG 1 cut(s) 233
PciSI GCTCTTC 1 cut(s) 188
PcsI WCGNNNNNNNCGW 4 cut(s) 99, 159, 210, 266
PkrI GCNGC 4 cut(s) 73, 175, 210, 227
Ple19I CGATCG 1 cut(s) 297
PmaCI CACGTG 1 cut(s) 322
PmlI CACGTG 1 cut(s) 322
Ppu21I YACGTR 1 cut(s) 322
Psp124BI GAGCTC 1 cut(s) 51
Psp6I CCWGG 2 cut(s) 176, 279
PspCI CACGTG 1 cut(s) 322
PspGI CCWGG 2 cut(s) 176, 279
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 1 cut(s) 383
PsuI RGATCY 1 cut(s) 392
PvuI CGATCG 1 cut(s) 297
RseI CAYNNNNRTG 3 cut(s) 164, 233, 281
SacI GAGCTC 1 cut(s) 51
SapI GCTCTTC 1 cut(s) 188
SatI GCNGC 4 cut(s) 72, 174, 209, 226
Sau3AI GATC 3 cut(s) 141, 294, 392
Sau96I GGNCC 1 cut(s) 383
ScrFI CCNGG 3 cut(s) 178, 281, 386
SduI GDGCHC 2 cut(s) 51, 321
SetI ASST 7 cut(s) 26, 51, 141, 263, 324, 354, 403
SfaNI GCATC 2 cut(s) 156, 315
SmiMI CAYNNNNRTG 3 cut(s) 164, 233, 281
SsiI CCGC 6 cut(s) 13, 61, 72, 82, 174, 209
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 3 cut(s) 176, 279, 384
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 3 cut(s) 263, 324, 354
TaqI TCGA 3 cut(s) 6, 39, 293
TauI GCSGC 3 cut(s) 74, 176, 211
TscAI CASTG 4 cut(s) 38, 235, 306, 373
TseI GCWGC 1 cut(s) 225
TspDTI ATGAA 2 cut(s) 101, 287
TspRI CASTG 4 cut(s) 38, 235, 306, 373
VneI GTGCAC 1 cut(s) 317
ZraI GACGTC 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.