FvH4_6g33130

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013966
Physical Location & Seq
Reverse (-)
26201635 .. 26202201
567 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g33130.t1

Sequence Viewer

Length: 567 bp
ATGACGACGTACGGCACCATCCCGACCTCGTCGTCGCCGTCGGGTTCCACCTCCGCCTCCGGCGAGTACATCGCCCGAGCCAAGGACCGTATCAAGGCCGGCCTCGGCGCCCGCCGCCCCTGGAAGCTCATGTTCGACTTCCGCTCCCTCAATTTCGCCCACGGCTTCGGCGACGCGCTCACGCGCCTCCGCTACAACGCCTCCTACTTCCGCATGAACTACGCCATCATCGTCCTCCTGATCCTCTTCCTCAGCCTCCTCTGGCACCCGATCTCGCTCATCGTCCTCATCGTGATGCTCGCCGCCTGGCTCTTCCTCTACTTCCTCCGCGACGAGCCGCTCGTCCTCTTCGGCCGCACCGTCGACGACCGCATCGTCCTGATCGTCCTCAGCGTCGTGACGATTGTGCTTCTCTTTCTGACGTCGGCCACGATGAACATCCTGGTGGCGCTGCTGATCTCGGTGGTGGTCGTGGCGGTGCACGCCACGCTGAGGAAGACGGAGGATTTGCACGTTGATGAGGAGAACGCTGGATTGATGACCGGCCCGGCTGGATCTTCAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

189

Amino Acids

20.75

Weight (kDa)

9.39

Isoelectric Point (pI)

29.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 35 - 175 6.5e-51 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013966)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17700
fragaria_vesca FvH4_1g19140 FvH4_6g04220 FvH4_6g33130
malus_domestica MD14G1066400.v1.1 MD17G1045700.v1.1 MD17G1175300.v1.1
prunus_persica Prupe.3G051300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0142821
rosa_laevigata RLG00000019988
rosa_multiflora Rmu_sc0002358.1_g000017
rosa_roxburghii Rroxscaffold_2G00102290 Rroxscaffold_2G00103340
rosa_rugosa Rorug02G0375500
rosa_samantha Rh2AG428600 Rh2CG414200
rosa_wichuraiana Rw2G035050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 31, 374
AatII GACGTC 1 cut(s) 425
AccB1I GGYRCC 3 cut(s) 14, 107, 264
AccBSI CCGCTC 2 cut(s) 144, 340
AccI GTMKAC 1 cut(s) 363
AccII CGCG 3 cut(s) 176, 184, 330
AclWI GGATC 2 cut(s) 235, 562
AcoI YGGCCR 2 cut(s) 352, 426
AcuI CTGAAG 1 cut(s) 543
AcyI GRCGYC 2 cut(s) 108, 422
AfaI GTAC 2 cut(s) 11, 68
AfiI CCNNNNNNNGG 3 cut(s) 82, 94, 492
AjnI CCWGG 3 cut(s) 119, 305, 441
AluBI AGCT 2 cut(s) 127, 563
AluI AGCT 2 cut(s) 127, 563
Alw21I GWGCWC 1 cut(s) 483
Alw44I GTGCAC 1 cut(s) 479
AlwI GGATC 2 cut(s) 235, 562
Ama87I CYCGRG 1 cut(s) 75
AoxI GGCC 5 cut(s) 96, 100, 352, 426, 544
ApaLI GTGCAC 1 cut(s) 479
ApeKI GCWGC 1 cut(s) 451
ArsI GACNNNNNNTTYG 2 cut(s) 490, 522
AspLEI GCGC 4 cut(s) 110, 178, 186, 451
AspS9I GGNCC 2 cut(s) 85, 545
AsuC2I CCSGG 1 cut(s) 548
AvaI CYCGRG 1 cut(s) 75
AvaII GGWCC 1 cut(s) 85
BaeGI GKGCMC 1 cut(s) 483
BanI GGYRCC 3 cut(s) 14, 107, 264
BbsI GAAGAC 1 cut(s) 503
Bbv12I GWGCWC 1 cut(s) 483
BbvCI CCTCAGC 3 cut(s) 251, 389, 491
BbvI GCAGC 1 cut(s) 438
BccI CCATC 2 cut(s) 26, 233
BceAI ACGGC 3 cut(s) 22, 28, 178
BciT130I CCWGG 3 cut(s) 121, 307, 443
BcnI CCSGG 1 cut(s) 548
BfoI RGCGCY 2 cut(s) 111, 452
BisI GCNGC 5 cut(s) 115, 303, 338, 355, 452
BlsI GCNGC 5 cut(s) 116, 304, 339, 356, 453
Bme1390I CCNGG 4 cut(s) 121, 307, 443, 548
Bme18I GGWCC 1 cut(s) 85
BmeT110I CYCGRG 1 cut(s) 75
BmgT120I GGNCC 2 cut(s) 85, 545
BmiI GGNNCC 4 cut(s) 16, 46, 109, 266
BmrFI CCNGG 4 cut(s) 121, 307, 443, 548
BmsI GCATC 2 cut(s) 285, 381
BpiI GAAGAC 1 cut(s) 503
Bpu10I CCTNAGC 3 cut(s) 251, 389, 491
BpuMI CCSGG 1 cut(s) 548
BsaBI GATNNNNATC 1 cut(s) 437
BsaHI GRCGYC 2 cut(s) 108, 422
BsaJI CCNNGG 4 cut(s) 81, 103, 119, 160
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 185, 215
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 94, 492
Bse118I RCCGGY 2 cut(s) 98, 542
Bse8I GATNNNNATC 1 cut(s) 437
BseBI CCWGG 3 cut(s) 121, 307, 443
BseDI CCNNGG 4 cut(s) 81, 103, 119, 160
BseGI GGATG 2 cut(s) 18, 438
BseJI GATNNNNATC 1 cut(s) 437
BseLI CCNNNNNNNGG 3 cut(s) 82, 94, 492
BseMII CTCAG 3 cut(s) 265, 403, 482
BseRI GAGGAG 2 cut(s) 248, 536
BseSI GKGCMC 1 cut(s) 483
BseX3I CGGCCG 1 cut(s) 352
BseXI GCAGC 1 cut(s) 438
Bsh1236I CGCG 3 cut(s) 176, 184, 330
Bsh1285I CGRYCG 2 cut(s) 355, 370
BshFI GGCC 5 cut(s) 98, 102, 354, 428, 546
BshNI GGYRCC 3 cut(s) 14, 107, 264
BsiEI CGRYCG 2 cut(s) 355, 370
BsiHKAI GWGCWC 1 cut(s) 483
BsiHKCI CYCGRG 1 cut(s) 75
BsiSI CCGG 4 cut(s) 60, 99, 543, 548
BsiWI CGTACG 1 cut(s) 9
BslI CCNNNNNNNGG 3 cut(s) 82, 94, 492
BsnI GGCC 5 cut(s) 98, 102, 354, 428, 546
BsoBI CYCGRG 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 483
Bsp143I GATC 5 cut(s) 240, 270, 381, 456, 554
BspANI GGCC 5 cut(s) 98, 102, 354, 428, 546
BspCNI CTCAG 3 cut(s) 264, 402, 483
BspFNI CGCG 3 cut(s) 176, 184, 330
BspLI GGNNCC 4 cut(s) 16, 46, 109, 266
BspPI GGATC 2 cut(s) 235, 562
BspQI GCTCTTC 1 cut(s) 317
BspT107I GGYRCC 3 cut(s) 14, 107, 264
BsrBI CCGCTC 2 cut(s) 144, 340
BsrFI RCCGGY 2 cut(s) 98, 542
BssAI RCCGGY 2 cut(s) 98, 542
BssECI CCNNGG 4 cut(s) 81, 103, 119, 160
BssMI GATC 5 cut(s) 240, 270, 381, 456, 554
BssNI GRCGYC 2 cut(s) 108, 422
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 3 cut(s) 121, 307, 443
Bst4CI ACNGT 2 cut(s) 89, 361
Bst6I CTCTTC 3 cut(s) 251, 317, 353
BstACI GRCGYC 2 cut(s) 108, 422
BstC8I GCNNGC 4 cut(s) 100, 112, 300, 483
BstDEI CTNAG 4 cut(s) 251, 389, 491, 564
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 2 cut(s) 18, 438
BstFNI CGCG 3 cut(s) 176, 184, 330
BstH2I RGCGCY 2 cut(s) 111, 452
BstHHI GCGC 4 cut(s) 110, 178, 186, 451
BstKTI GATC 5 cut(s) 243, 273, 384, 459, 557
BstMBI GATC 5 cut(s) 240, 270, 381, 456, 554
BstMCI CGRYCG 2 cut(s) 355, 370
BstMWI GCNNNNNNNGC 3 cut(s) 114, 482, 487
BstNI CCWGG 3 cut(s) 121, 307, 443
BstSCI CCNGG 4 cut(s) 119, 305, 441, 546
BstSLI GKGCMC 1 cut(s) 483
BstUI CGCG 3 cut(s) 176, 184, 330
BstV1I GCAGC 1 cut(s) 438
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 1 cut(s) 554
BstYI RGATCY 1 cut(s) 554
BstZI CGGCCG 1 cut(s) 352
BsuRI GGCC 5 cut(s) 98, 102, 354, 428, 546
BtgI CCRYGG 1 cut(s) 160
BtgZI GCGATG 1 cut(s) 55
BtsCI GGATG 2 cut(s) 18, 438
Cac8I GCNNGC 4 cut(s) 100, 112, 300, 483
CfoI GCGC 4 cut(s) 110, 178, 186, 451
Cfr10I RCCGGY 2 cut(s) 98, 542
Cfr13I GGNCC 2 cut(s) 85, 545
CseI GACGC 2 cut(s) 182, 382
Csp6I GTAC 2 cut(s) 10, 67
CviAII CATG 2 cut(s) 130, 214
CviQI GTAC 2 cut(s) 10, 67
DdeI CTNAG 4 cut(s) 251, 389, 491, 564
DinI GGCGCC 1 cut(s) 109
DpnI GATC 5 cut(s) 242, 272, 383, 458, 556
DpnII GATC 5 cut(s) 240, 270, 381, 456, 554
DrdI GACNNNNNNGTC 2 cut(s) 31, 374
DseDI GACNNNNNNGTC 2 cut(s) 31, 374
EaeI YGGCCR 2 cut(s) 352, 426
EagI CGGCCG 1 cut(s) 352
Eam1104I CTCTTC 3 cut(s) 251, 317, 353
EarI CTCTTC 3 cut(s) 251, 317, 353
EciI GGCGGA 1 cut(s) 43
EclXI CGGCCG 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 85
Eco52I CGGCCG 1 cut(s) 352
Eco57I CTGAAG 1 cut(s) 543
Eco88I CYCGRG 1 cut(s) 75
EcoRII CCWGG 3 cut(s) 119, 305, 441
EcoT14I CCWWGG 1 cut(s) 81
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 2 cut(s) 133, 217
FaiI YATR 2 cut(s) 131, 215
FatI CATG 2 cut(s) 129, 213
FauI CCCGC 1 cut(s) 119
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 5 cut(s) 115, 303, 338, 355, 452
FokI GGATG 2 cut(s) 5, 425
FseI GGCCGGCC 1 cut(s) 102
Fsp4HI GCNGC 5 cut(s) 115, 303, 338, 355, 452
GlaI GCGC 4 cut(s) 109, 177, 185, 450
GluI GCNGC 5 cut(s) 115, 303, 338, 355, 452
HaeII RGCGCY 2 cut(s) 111, 452
HaeIII GGCC 5 cut(s) 98, 102, 354, 428, 546
HapII CCGG 4 cut(s) 60, 99, 543, 548
HgaI GACGC 2 cut(s) 182, 382
HhaI GCGC 4 cut(s) 110, 178, 186, 451
Hin1I GRCGYC 2 cut(s) 108, 422
Hin1II CATG 2 cut(s) 133, 217
Hin6I GCGC 4 cut(s) 108, 176, 184, 449
HinP1I GCGC 4 cut(s) 108, 176, 184, 449
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HpaII CCGG 4 cut(s) 60, 99, 543, 548
Hpy166II GTNNAC 2 cut(s) 364, 481
Hpy188I TCNGA 1 cut(s) 420
Hpy188III TCNNGA 5 cut(s) 22, 238, 292, 379, 397
Hpy8I GTNNAC 2 cut(s) 364, 481
HpyCH4III ACNGT 2 cut(s) 89, 361
HpyCH4IV ACGT 3 cut(s) 8, 422, 513
HpyCH4V TGCA 2 cut(s) 481, 511
HpyF10VI GCNNNNNNNGC 3 cut(s) 114, 482, 487
HpyF3I CTNAG 4 cut(s) 251, 389, 491, 564
HpySE526I ACGT 3 cut(s) 8, 422, 513
Hsp92I GRCGYC 2 cut(s) 108, 422
Hsp92II CATG 2 cut(s) 133, 217
HspAI GCGC 4 cut(s) 108, 176, 184, 449
KasI GGCGCC 1 cut(s) 107
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Kzo9I GATC 5 cut(s) 240, 270, 381, 456, 554
LguI GCTCTTC 1 cut(s) 317
LmnI GCTCC 1 cut(s) 149
Lsp1109I GCAGC 1 cut(s) 438
LweI GCATC 2 cut(s) 285, 381
MaeII ACGT 3 cut(s) 8, 422, 513
MaeIII GTNAC 1 cut(s) 397
MalI GATC 5 cut(s) 242, 272, 383, 458, 556
MbiI CCGCTC 2 cut(s) 144, 340
MboI GATC 5 cut(s) 240, 270, 381, 456, 554
MboII GAAGA 5 cut(s) 238, 304, 340, 508, 549
MflI RGATCY 1 cut(s) 554
MhlI GDGCHC 1 cut(s) 483
MluCI AATT 1 cut(s) 151
Mly113I GGCGCC 1 cut(s) 108
MroNI GCCGGC 1 cut(s) 98
MslI CAYNNNNRTG 3 cut(s) 293, 443, 516
MspI CCGG 4 cut(s) 60, 99, 543, 548
MspR9I CCNGG 4 cut(s) 121, 307, 443, 548
MvaI CCWGG 3 cut(s) 121, 307, 443
MvnI CGCG 3 cut(s) 176, 184, 330
MwoI GCNNNNNNNGC 3 cut(s) 114, 482, 487
NaeI GCCGGC 1 cut(s) 100
NarI GGCGCC 1 cut(s) 108
NciI CCSGG 1 cut(s) 548
NdeII GATC 5 cut(s) 240, 270, 381, 456, 554
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 2 cut(s) 133, 217
NlaIV GGNNCC 4 cut(s) 16, 46, 109, 266
NmeAIII GCCGAG 1 cut(s) 84
NmuCI GTSAC 1 cut(s) 397
PciSI GCTCTTC 1 cut(s) 317
PdiI GCCGGC 1 cut(s) 100
Pfl23II CGTACG 1 cut(s) 9
PflFI GACNNNGTC 1 cut(s) 28
PkrI GCNGC 5 cut(s) 116, 304, 339, 356, 453
PluTI GGCGCC 1 cut(s) 111
Psp6I CCWGG 3 cut(s) 119, 305, 441
PspGI CCWGG 3 cut(s) 119, 305, 441
PspLI CGTACG 1 cut(s) 9
PspN4I GGNNCC 4 cut(s) 16, 46, 109, 266
PspPI GGNCC 2 cut(s) 85, 545
PsuI RGATCY 1 cut(s) 554
PsyI GACNNNGTC 1 cut(s) 28
RigI GGCCGGCC 1 cut(s) 102
RsaI GTAC 2 cut(s) 11, 68
RsaNI GTAC 2 cut(s) 10, 67
RseI CAYNNNNRTG 3 cut(s) 293, 443, 516
SalI GTCGAC 1 cut(s) 362
SapI GCTCTTC 1 cut(s) 317
SatI GCNGC 5 cut(s) 115, 303, 338, 355, 452
Sau3AI GATC 5 cut(s) 240, 270, 381, 456, 554
Sau96I GGNCC 2 cut(s) 85, 545
ScrFI CCNGG 4 cut(s) 121, 307, 443, 548
SduI GDGCHC 1 cut(s) 483
SetI ASST 7 cut(s) 11, 29, 53, 129, 425, 516, 565
SfaNI GCATC 2 cut(s) 285, 381
SfoI GGCGCC 1 cut(s) 109
SgrDI CGTCGACG 1 cut(s) 362
SinI GGWCC 1 cut(s) 85
SmiMI CAYNNNNRTG 3 cut(s) 293, 443, 516
Sse9I AATT 1 cut(s) 151
SspDI GGCGCC 1 cut(s) 107
StyD4I CCNGG 4 cut(s) 119, 305, 441, 546
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 2 cut(s) 89, 361
TaiI ACGT 3 cut(s) 11, 425, 516
TaqI TCGA 2 cut(s) 135, 363
TasI AATT 1 cut(s) 151
TatI WGTACW 1 cut(s) 66
TauI GCSGC 4 cut(s) 117, 305, 340, 357
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 1 cut(s) 451
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 2 cut(s) 230, 449
TspGWI ACGGA 1 cut(s) 515
Tth111I GACNNNGTC 1 cut(s) 28
VneI GTGCAC 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 85
XmiI GTMKAC 1 cut(s) 363
ZraI GACGTC 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.