MD17G1175300.v1.1

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013966
Physical Location & Seq
Forward (+)
19638205 .. 19640656
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1175300.v1.1.491

Sequence Viewer

Length: 561 bp
ATGACCACCTACGGCACGATCCCGACCTCCTCCTCGTCTTCCCAAGGCCCCTCCGGCGAGTACATCTCACGCGCCAAGGACCGCATCAGGGCCGGCCTCGGCGCCCGCCGGCCATGGAAGCTGATGTTCAACTTCCGGTCCTTCAACTTCCCCTCCTCCTTCTCCGACGCCTTCGGGCGGGTCCGATCGAACGTCGCCTACTTCCGGATGAACTATGCCATCGTGGTGCTCCTGATCCTGTTCCTCAGCCTGCTCTGGCACCCGATCTCGCTCATCGTCTTCGTCGTCATGATGGCCGCCTGGCTCTTCCTCTACTTCCTCCGCGACGAGCCGCTGTTGCTGTTCGGAAAAACGGTAGACGACCGCGTCGTTTTGATCGTGCTGGCGGTGGTGACGATCGTGCTGCTGTTCTTAACCCACGCGACGGTGAATATTCTGGTGGCGCTGCTGATTGGGGTTGTGTTGGTGATTGCGCACGCGGCGTTGAGGAAGATGGATGACTTGCCGGTGGACGAAGAGAACTCTGCGTTGTTGACTACGCCTGCATCTTCCTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

187

Amino Acids

20.67

Weight (kDa)

9.69

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 33 - 173 2.6e-52 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013966)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17700
fragaria_vesca FvH4_1g19140 FvH4_6g04220 FvH4_6g33130
malus_domestica MD14G1066400.v1.1 MD17G1045700.v1.1 MD17G1175300.v1.1
prunus_persica Prupe.3G051300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0142821
rosa_laevigata RLG00000019988
rosa_multiflora Rmu_sc0002358.1_g000017
rosa_roxburghii Rroxscaffold_2G00102290 Rroxscaffold_2G00103340
rosa_rugosa Rorug02G0375500
rosa_samantha Rh2AG428600 Rh2CG414200
rosa_wichuraiana Rw2G035050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 365
Acc16I TGCGCA 1 cut(s) 474
AccB1I GGYRCC 2 cut(s) 101, 258
AccI GTMKAC 1 cut(s) 357
AccII CGCG 5 cut(s) 72, 324, 366, 422, 479
AccIII TCCGGA 1 cut(s) 204
AciI CCGC 9 cut(s) 82, 106, 178, 297, 322, 332, 364, 386, 479
AclWI GGATC 2 cut(s) 13, 229
AcoI YGGCCR 2 cut(s) 110, 294
AcyI GRCGYC 2 cut(s) 102, 168
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 4 cut(s) 88, 177, 204, 424
AgsI TTSAA 2 cut(s) 130, 145
AjnI CCWGG 1 cut(s) 299
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
Alw21I GWGCWC 1 cut(s) 231
AlwI GGATC 2 cut(s) 13, 229
Aor13HI TCCGGA 1 cut(s) 204
AoxI GGCC 5 cut(s) 46, 90, 94, 110, 294
ApeKI GCWGC 2 cut(s) 403, 445
AspLEI GCGC 4 cut(s) 74, 104, 445, 475
AspS9I GGNCC 5 cut(s) 47, 79, 90, 138, 181
AsuHPI GGTGA 3 cut(s) 403, 439, 478
AvaII GGWCC 3 cut(s) 79, 138, 181
BanI GGYRCC 2 cut(s) 101, 258
BbsI GAAGAC 2 cut(s) 30, 271
Bbv12I GWGCWC 1 cut(s) 231
BbvCI CCTCAGC 1 cut(s) 245
BbvI GCAGC 2 cut(s) 390, 432
BccI CCATC 3 cut(s) 227, 286, 487
BceAI ACGGC 1 cut(s) 28
BcgI CGANNNNNNTGC 2 cut(s) 385, 419
BciT130I CCWGG 1 cut(s) 301
BfoI RGCGCY 2 cut(s) 105, 446
BglI GCCNNNNNGGC 1 cut(s) 54
BisI GCNGC 5 cut(s) 297, 332, 404, 446, 480
BlsI GCNGC 5 cut(s) 298, 333, 405, 447, 481
Bme1390I CCNGG 1 cut(s) 301
Bme18I GGWCC 3 cut(s) 79, 138, 181
BmgT120I GGNCC 5 cut(s) 47, 79, 90, 138, 181
BmiI GGNNCC 4 cut(s) 49, 103, 182, 260
BmrFI CCNGG 1 cut(s) 301
BmsI GCATC 2 cut(s) 93, 554
BpiI GAAGAC 2 cut(s) 30, 271
BplI GAGNNNNNCTC 2 cut(s) 50, 82
Bpu10I CCTNAGC 1 cut(s) 245
BsaHI GRCGYC 2 cut(s) 102, 168
BsaJI CCNNGG 4 cut(s) 43, 75, 97, 113
BsaWI WCCGGW 2 cut(s) 135, 204
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 177, 204, 424
Bse118I RCCGGY 3 cut(s) 92, 108, 505
BseAI TCCGGA 1 cut(s) 204
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 4 cut(s) 43, 75, 97, 113
BseGI GGATG 2 cut(s) 213, 502
BseLI CCNNNNNNNGG 4 cut(s) 88, 177, 204, 424
BseMII CTCAG 1 cut(s) 259
BseRI GAGGAG 3 cut(s) 19, 22, 145
BseXI GCAGC 2 cut(s) 390, 432
Bsh1236I CGCG 5 cut(s) 72, 324, 366, 422, 479
Bsh1285I CGRYCG 3 cut(s) 188, 364, 399
BshFI GGCC 5 cut(s) 48, 92, 96, 112, 296
BshNI GGYRCC 2 cut(s) 101, 258
BsiEI CGRYCG 3 cut(s) 188, 364, 399
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 6 cut(s) 54, 93, 109, 136, 205, 506
BslI CCNNNNNNNGG 4 cut(s) 88, 177, 204, 424
BsnI GGCC 5 cut(s) 48, 92, 96, 112, 296
Bsp1286I GDGCHC 1 cut(s) 231
Bsp13I TCCGGA 1 cut(s) 204
Bsp143I GATC 6 cut(s) 18, 185, 234, 264, 375, 396
Bsp19I CCATGG 1 cut(s) 113
BspACI CCGC 9 cut(s) 82, 106, 178, 297, 322, 332, 364, 386, 479
BspANI GGCC 5 cut(s) 48, 92, 96, 112, 296
BspCNI CTCAG 1 cut(s) 258
BspEI TCCGGA 1 cut(s) 204
BspFNI CGCG 5 cut(s) 72, 324, 366, 422, 479
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 4 cut(s) 49, 103, 182, 260
BspPI GGATC 2 cut(s) 13, 229
BspQI GCTCTTC 1 cut(s) 311
BspT107I GGYRCC 2 cut(s) 101, 258
BsrFI RCCGGY 3 cut(s) 92, 108, 505
BssAI RCCGGY 3 cut(s) 92, 108, 505
BssECI CCNNGG 4 cut(s) 43, 75, 97, 113
BssMI GATC 6 cut(s) 18, 185, 234, 264, 375, 396
BssNI GRCGYC 2 cut(s) 102, 168
BssT1I CCWWGG 3 cut(s) 43, 75, 113
Bst2UI CCWGG 1 cut(s) 301
Bst4CI ACNGT 2 cut(s) 355, 427
Bst6I CTCTTC 2 cut(s) 311, 510
BstACI GRCGYC 2 cut(s) 102, 168
BstC8I GCNNGC 7 cut(s) 94, 106, 110, 251, 384, 477, 543
BstDEI CTNAG 1 cut(s) 245
BstDSI CCRYGG 1 cut(s) 113
BstF5I GGATG 2 cut(s) 213, 502
BstFNI CGCG 5 cut(s) 72, 324, 366, 422, 479
BstH2I RGCGCY 2 cut(s) 105, 446
BstHHI GCGC 4 cut(s) 74, 104, 445, 475
BstKTI GATC 6 cut(s) 21, 188, 237, 267, 378, 399
BstMBI GATC 6 cut(s) 18, 185, 234, 264, 375, 396
BstMCI CGRYCG 3 cut(s) 188, 364, 399
BstMWI GCNNNNNNNGC 4 cut(s) 54, 118, 337, 479
BstNI CCWGG 1 cut(s) 301
BstSCI CCNGG 1 cut(s) 299
BstUI CGCG 5 cut(s) 72, 324, 366, 422, 479
BstV1I GCAGC 2 cut(s) 390, 432
BstV2I GAAGAC 2 cut(s) 30, 271
BsuRI GGCC 5 cut(s) 48, 92, 96, 112, 296
BtgI CCRYGG 1 cut(s) 113
BtsCI GGATG 2 cut(s) 213, 502
Cac8I GCNNGC 7 cut(s) 94, 106, 110, 251, 384, 477, 543
CciI TCATGA 1 cut(s) 288
CfoI GCGC 4 cut(s) 74, 104, 445, 475
Cfr10I RCCGGY 3 cut(s) 92, 108, 505
Cfr13I GGNCC 5 cut(s) 47, 79, 90, 138, 181
CseI GACGC 2 cut(s) 176, 355
Csp6I GTAC 1 cut(s) 61
CviAII CATG 2 cut(s) 114, 289
CviJI RGCY 9 cut(s) 48, 92, 96, 112, 121, 249, 296, 304, 331
CviKI_1 RGCY 9 cut(s) 48, 92, 96, 112, 121, 249, 296, 304, 331
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 1 cut(s) 245
DinI GGCGCC 1 cut(s) 103
DpnI GATC 6 cut(s) 20, 187, 236, 266, 377, 398
DpnII GATC 6 cut(s) 18, 185, 234, 264, 375, 396
DrdI GACNNNNNNGTC 1 cut(s) 365
DseDI GACNNNNNNGTC 1 cut(s) 365
EaeI YGGCCR 2 cut(s) 110, 294
Eam1104I CTCTTC 2 cut(s) 311, 510
EarI CTCTTC 2 cut(s) 311, 510
Eco130I CCWWGG 3 cut(s) 43, 75, 113
Eco47I GGWCC 3 cut(s) 79, 138, 181
EcoO109I RGGNCCY 1 cut(s) 47
EcoRII CCWGG 1 cut(s) 299
EcoT14I CCWWGG 3 cut(s) 43, 75, 113
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
ErhI CCWWGG 3 cut(s) 43, 75, 113
FaeI CATG 2 cut(s) 117, 292
FaiI YATR 3 cut(s) 115, 216, 290
FatI CATG 2 cut(s) 113, 288
FauI CCCGC 2 cut(s) 113, 171
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 5 cut(s) 297, 332, 404, 446, 480
FokI GGATG 2 cut(s) 220, 509
FseI GGCCGGCC 1 cut(s) 96
Fsp4HI GCNGC 5 cut(s) 297, 332, 404, 446, 480
FspI TGCGCA 1 cut(s) 474
GlaI GCGC 4 cut(s) 73, 103, 444, 474
GluI GCNGC 5 cut(s) 297, 332, 404, 446, 480
HaeII RGCGCY 2 cut(s) 105, 446
HaeIII GGCC 5 cut(s) 48, 92, 96, 112, 296
HapII CCGG 6 cut(s) 54, 93, 109, 136, 205, 506
HgaI GACGC 2 cut(s) 176, 355
HhaI GCGC 4 cut(s) 74, 104, 445, 475
Hin1I GRCGYC 2 cut(s) 102, 168
Hin1II CATG 2 cut(s) 117, 292
Hin6I GCGC 4 cut(s) 72, 102, 443, 473
HinP1I GCGC 4 cut(s) 72, 102, 443, 473
HincII GTYRAC 1 cut(s) 534
HindII GTYRAC 1 cut(s) 534
HpaII CCGG 6 cut(s) 54, 93, 109, 136, 205, 506
HphI GGTGA 3 cut(s) 403, 439, 478
Hpy166II GTNNAC 3 cut(s) 358, 511, 534
Hpy188I TCNGA 3 cut(s) 166, 185, 347
Hpy188III TCNNGA 4 cut(s) 22, 205, 232, 289
Hpy8I GTNNAC 3 cut(s) 358, 511, 534
Hpy99I CGWCG 6 cut(s) 170, 197, 287, 329, 371, 427
HpyAV CCTTC 3 cut(s) 151, 169, 181
HpyCH4III ACNGT 2 cut(s) 355, 427
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 1 cut(s) 545
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 118, 337, 479
HpyF3I CTNAG 1 cut(s) 245
HpySE526I ACGT 1 cut(s) 192
Hsp92I GRCGYC 2 cut(s) 102, 168
Hsp92II CATG 2 cut(s) 117, 292
HspAI GCGC 4 cut(s) 72, 102, 443, 473
KasI GGCGCC 1 cut(s) 101
Kpn2I TCCGGA 1 cut(s) 204
KroI GCCGGC 2 cut(s) 92, 108
KroNI GCCGGC 2 cut(s) 94, 110
Kzo9I GATC 6 cut(s) 18, 185, 234, 264, 375, 396
LguI GCTCTTC 1 cut(s) 311
LmnI GCTCC 1 cut(s) 234
Lsp1109I GCAGC 2 cut(s) 390, 432
LweI GCATC 2 cut(s) 93, 554
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 1 cut(s) 391
MalI GATC 6 cut(s) 20, 187, 236, 266, 377, 398
MboI GATC 6 cut(s) 18, 185, 234, 264, 375, 396
MboII GAAGA 6 cut(s) 30, 271, 298, 502, 527, 540
MhlI GDGCHC 1 cut(s) 231
Mly113I GGCGCC 1 cut(s) 102
MmeI TCCRAC 1 cut(s) 189
MroI TCCGGA 1 cut(s) 204
MroNI GCCGGC 2 cut(s) 92, 108
MseI TTAA 2 cut(s) 413, 559
MslI CAYNNNNRTG 1 cut(s) 224
MspA1I CMGCKG 1 cut(s) 334
MspI CCGG 6 cut(s) 54, 93, 109, 136, 205, 506
MspR9I CCNGG 1 cut(s) 301
MvaI CCWGG 1 cut(s) 301
MvnI CGCG 5 cut(s) 72, 324, 366, 422, 479
MwoI GCNNNNNNNGC 4 cut(s) 54, 118, 337, 479
NaeI GCCGGC 2 cut(s) 94, 110
NarI GGCGCC 1 cut(s) 102
NcoI CCATGG 1 cut(s) 113
NdeII GATC 6 cut(s) 18, 185, 234, 264, 375, 396
NgoMIV GCCGGC 2 cut(s) 92, 108
NlaIII CATG 2 cut(s) 117, 292
NlaIV GGNNCC 4 cut(s) 49, 103, 182, 260
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 391
NsbI TGCGCA 1 cut(s) 474
PagI TCATGA 1 cut(s) 288
PciSI GCTCTTC 1 cut(s) 311
PcsI WCGNNNNNNNCGW 3 cut(s) 282, 366, 375
PdiI GCCGGC 2 cut(s) 94, 110
PflFI GACNNNGTC 1 cut(s) 365
PkrI GCNGC 5 cut(s) 298, 333, 405, 447, 481
Ple19I CGATCG 2 cut(s) 188, 399
PluTI GGCGCC 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 4 cut(s) 49, 103, 182, 260
PspPI GGNCC 5 cut(s) 47, 79, 90, 138, 181
PsrI GAACNNNNNNTAC 2 cut(s) 182, 214
PsyI GACNNNGTC 1 cut(s) 365
PvuI CGATCG 2 cut(s) 188, 399
RigI GGCCGGCC 1 cut(s) 96
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
RseI CAYNNNNRTG 1 cut(s) 224
SapI GCTCTTC 1 cut(s) 311
SaqAI TTAA 2 cut(s) 413, 559
SatI GCNGC 5 cut(s) 297, 332, 404, 446, 480
Sau3AI GATC 6 cut(s) 18, 185, 234, 264, 375, 396
Sau96I GGNCC 5 cut(s) 47, 79, 90, 138, 181
ScrFI CCNGG 1 cut(s) 301
SduI GDGCHC 1 cut(s) 231
SetI ASST 4 cut(s) 11, 29, 123, 195
SfaNI GCATC 2 cut(s) 93, 554
SfoI GGCGCC 1 cut(s) 103
SinI GGWCC 3 cut(s) 79, 138, 181
SmiMI CAYNNNNRTG 1 cut(s) 224
SsiI CCGC 9 cut(s) 82, 106, 178, 297, 322, 332, 364, 386, 479
SspDI GGCGCC 1 cut(s) 101
SspI AATATT 1 cut(s) 433
StyD4I CCNGG 1 cut(s) 299
StyI CCWWGG 3 cut(s) 43, 75, 113
TaaI ACNGT 2 cut(s) 355, 427
TaiI ACGT 1 cut(s) 195
TaqI TCGA 1 cut(s) 188
TatI WGTACW 1 cut(s) 60
TauI GCSGC 3 cut(s) 299, 334, 482
Tru1I TTAA 2 cut(s) 413, 559
Tru9I TTAA 2 cut(s) 413, 559
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 2 cut(s) 403, 445
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 1 cut(s) 224
Tth111I GACNNNGTC 1 cut(s) 365
VpaK11BI GGWCC 3 cut(s) 79, 138, 181
XmiI GTMKAC 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.