FvH4_6g05361

Glycine-rich RNA-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013848
Physical Location & Seq
Reverse (-)
3019583 .. 3020606
1024 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g05361.t1

Sequence Viewer

Length: 672 bp
ATGTCGGAAGAGCCTGAGTACCGTTGCTTTATTGGTGGCCTTGCCTGGGCGACATCTGATAGGAGTCTAAAAGAAGCGTTTGACAAGTATGGAAAGCTTATTGAAGCCAAGGTTGTTATGGATAAGTTCTCGGGGCGCTCTCGTGGATTTGGGTTCGTCACTTTTGATGATAAAAAAGCTATGGAAGAGGCAATTGAGGAAATGAATGGTATTGATTTAGATGGACGAACTATCACTGTTGATAAAGCTCAGCCTCACCAAGGTTCAGGTAGAGATTATGATGGCGACCGCGGCCGTGATCGTGGTGGCCGCGATCGTGACCGTGGTCGTGATTACGGAGGTGGTGGACGTGGATCTGGTGGGGGAGGAGATTGCTTCAAGTGTGGCAAGCCAGGACATTTTGCAAGGGAGTGTCCTAGTGAAGTATCAAGAGGAGGTGGTGGTGGCAGGTATGGTGGTAGGGATGATAAGTATAGTGGAGGAGGTGGTGGTGGGGGTGGTGGTAGCCGCTACAGCGGGGGGCCTGATCGAAATGGAGACCGATACAGTGGGAGCGGACGCAGCAGGGAGTCTGGTTCTGGTGGTCGTTCAAGCTCTGGAAGCGATCGTTTTAGCCGTGACCGCTCTGGTCCATATGAGCGTCGGGGGACAGGGAGTTTCCGTTCTGGATAG

Protein Analysis

224

Amino Acids

23.63

Weight (kDa)

9.17

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 10 - 79 5.5e-21 RNA recognition motif
zf-CCHC PF00098 125 - 140 1.1e-07 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26420
fragaria_vesca FvH4_6g05361
malus_domestica MD04G1208800.v1.1 MD12G1223200.v1.1
prunus_persica Prupe.6G329800_v2.0.a1 Prupe.6G329800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0454141
rosa_laevigata RLG00000025479
rosa_multiflora Rmu_sc0018584.1_g000002
rosa_roxburghii Rroxscaffold_165G00436920
rosa_rugosa Rorug02G0653500 Rorug02G0653500
rosa_samantha Rh3AG056200 Rh3BG057600 Rh3CG056900 Rh3DG058100
rosa_wichuraiana Rw3G004320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 438
AccBSI CCGCTC 2 cut(s) 555, 624
AccII CGCG 2 cut(s) 291, 312
AciI CCGC 7 cut(s) 289, 291, 310, 508, 516, 555, 622
AclWI GGATC 1 cut(s) 361
AcoI YGGCCR 2 cut(s) 292, 307
AfaI GTAC 1 cut(s) 20
AfiI CCNNNNNNNGG 2 cut(s) 46, 260
AgsI TTSAA 3 cut(s) 104, 379, 591
AjiI CACGTC 1 cut(s) 350
AjnI CCWGG 2 cut(s) 44, 391
AloI GAACNNNNNNTCC 1 cut(s) 646
AluBI AGCT 4 cut(s) 97, 179, 248, 594
AluI AGCT 4 cut(s) 97, 179, 248, 594
Alw26I GTCTC 1 cut(s) 531
AlwI GGATC 1 cut(s) 361
Ama87I CYCGRG 1 cut(s) 130
AoxI GGCC 4 cut(s) 37, 292, 307, 521
ApeKI GCWGC 1 cut(s) 561
AspLEI GCGC 1 cut(s) 138
AspS9I GGNCC 2 cut(s) 521, 629
AsuHPI GGTGA 1 cut(s) 248
AvaI CYCGRG 1 cut(s) 130
AvaII GGWCC 1 cut(s) 629
BauI CACGAG 1 cut(s) 141
BbvI GCAGC 1 cut(s) 573
BccI CCATC 2 cut(s) 215, 275
BceAI ACGGC 2 cut(s) 279, 600
BciT130I CCWGG 2 cut(s) 46, 393
BcoDI GTCTC 1 cut(s) 531
BfaI CTAG 1 cut(s) 417
BfmI CTRYAG 1 cut(s) 511
BfoI RGCGCY 1 cut(s) 139
BfuAI ACCTGC 1 cut(s) 438
BisI GCNGC 4 cut(s) 292, 310, 508, 562
BlpI GCTNAGC 1 cut(s) 249
BlsI GCNGC 4 cut(s) 293, 311, 509, 563
Bme1390I CCNGG 2 cut(s) 46, 393
Bme18I GGWCC 1 cut(s) 629
BmeT110I CYCGRG 1 cut(s) 130
BmgBI CACGTC 1 cut(s) 350
BmgT120I GGNCC 2 cut(s) 521, 629
BmiI GGNNCC 1 cut(s) 522
BmrFI CCNGG 2 cut(s) 46, 393
BoxI GACNNNNGTC 1 cut(s) 324
Bpu1102I GCTNAGC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 531
BsaJI CCNNGG 5 cut(s) 45, 108, 259, 289, 322
BsaXI ACNNNNNCTCC 1 cut(s) 646
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 260
BseBI CCWGG 2 cut(s) 46, 393
BseDI CCNNGG 5 cut(s) 45, 108, 259, 289, 322
BseGI GGATG 1 cut(s) 469
BseLI CCNNNNNNNGG 2 cut(s) 46, 260
BseMII CTCAG 2 cut(s) 6, 263
BseRI GAGGAG 3 cut(s) 381, 447, 495
BseX3I CGGCCG 1 cut(s) 292
BseXI GCAGC 1 cut(s) 573
Bsh1236I CGCG 2 cut(s) 291, 312
Bsh1285I CGRYCG 4 cut(s) 289, 295, 316, 607
BshFI GGCC 4 cut(s) 39, 294, 309, 523
BsiEI CGRYCG 4 cut(s) 289, 295, 316, 607
BsiHKCI CYCGRG 1 cut(s) 130
BslFI GGGAC 1 cut(s) 661
BslI CCNNNNNNNGG 2 cut(s) 46, 260
BsmAI GTCTC 1 cut(s) 531
BsmFI GGGAC 1 cut(s) 661
BsnI GGCC 4 cut(s) 39, 294, 309, 523
Bso31I GGTCTC 1 cut(s) 531
BsoBI CYCGRG 1 cut(s) 130
Bsp143I GATC 5 cut(s) 298, 313, 353, 526, 604
Bsp1720I GCTNAGC 1 cut(s) 249
BspACI CCGC 7 cut(s) 289, 291, 310, 508, 516, 555, 622
BspANI GGCC 4 cut(s) 39, 294, 309, 523
BspCNI CTCAG 2 cut(s) 7, 262
BspFNI CGCG 2 cut(s) 291, 312
BspLI GGNNCC 1 cut(s) 522
BspMI ACCTGC 1 cut(s) 438
BspPI GGATC 1 cut(s) 361
BspQI GCTCTTC 1 cut(s) 3
BspTNI GGTCTC 1 cut(s) 531
BsrBI CCGCTC 2 cut(s) 555, 624
BssECI CCNNGG 5 cut(s) 45, 108, 259, 289, 322
BssMI GATC 5 cut(s) 298, 313, 353, 526, 604
BssSI CACGAG 1 cut(s) 141
BssT1I CCWWGG 2 cut(s) 108, 259
Bst2BI CACGAG 1 cut(s) 141
Bst2UI CCWGG 2 cut(s) 46, 393
Bst4CI ACNGT 4 cut(s) 23, 238, 323, 548
Bst6I CTCTTC 2 cut(s) 3, 180
BstC8I GCNNGC 1 cut(s) 389
BstDEI CTNAG 2 cut(s) 15, 249
BstDSI CCRYGG 2 cut(s) 289, 322
BstENI CCTNNNNNAGG 1 cut(s) 258
BstF5I GGATG 1 cut(s) 469
BstFNI CGCG 2 cut(s) 291, 312
BstH2I RGCGCY 1 cut(s) 139
BstHHI GCGC 1 cut(s) 138
BstKTI GATC 5 cut(s) 301, 316, 356, 529, 607
BstMAI GTCTC 1 cut(s) 531
BstMBI GATC 5 cut(s) 298, 313, 353, 526, 604
BstMCI CGRYCG 4 cut(s) 289, 295, 316, 607
BstMWI GCNNNNNNNGC 5 cut(s) 291, 513, 561, 600, 621
BstNI CCWGG 2 cut(s) 46, 393
BstPAI GACNNNNGTC 1 cut(s) 324
BstSCI CCNGG 2 cut(s) 44, 391
BstSFI CTRYAG 1 cut(s) 511
BstUI CGCG 2 cut(s) 291, 312
BstV1I GCAGC 1 cut(s) 573
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
BstZI CGGCCG 1 cut(s) 292
BsuRI GGCC 4 cut(s) 39, 294, 309, 523
BtgI CCRYGG 2 cut(s) 289, 322
BtrI CACGTC 1 cut(s) 350
BtsCI GGATG 1 cut(s) 469
BtsIMutI CAGTG 2 cut(s) 234, 553
BveI ACCTGC 1 cut(s) 438
Cac8I GCNNGC 1 cut(s) 389
CfoI GCGC 1 cut(s) 138
Cfr13I GGNCC 2 cut(s) 521, 629
Cfr42I CCGCGG 1 cut(s) 292
CseI GACGC 2 cut(s) 567, 629
Csp6I GTAC 1 cut(s) 19
CviQI GTAC 1 cut(s) 19
DdeI CTNAG 2 cut(s) 15, 249
DpnI GATC 5 cut(s) 300, 315, 355, 528, 606
DpnII GATC 5 cut(s) 298, 313, 353, 526, 604
EaeI YGGCCR 2 cut(s) 292, 307
EagI CGGCCG 1 cut(s) 292
Eam1104I CTCTTC 2 cut(s) 3, 180
EarI CTCTTC 2 cut(s) 3, 180
EclXI CGGCCG 1 cut(s) 292
Eco130I CCWWGG 2 cut(s) 108, 259
Eco31I GGTCTC 1 cut(s) 531
Eco47I GGWCC 1 cut(s) 629
Eco52I CGGCCG 1 cut(s) 292
Eco88I CYCGRG 1 cut(s) 130
EcoNI CCTNNNNNAGG 1 cut(s) 258
EcoO109I RGGNCCY 1 cut(s) 521
EcoRII CCWGG 2 cut(s) 44, 391
EcoT14I CCWWGG 2 cut(s) 108, 259
ErhI CCWWGG 2 cut(s) 108, 259
FaiI YATR 8 cut(s) 90, 119, 182, 279, 453, 474, 634, 636
FaqI GGGAC 1 cut(s) 661
FauI CCCGC 1 cut(s) 509
FauNDI CATATG 1 cut(s) 634
Fnu4HI GCNGC 4 cut(s) 292, 310, 508, 562
FokI GGATG 1 cut(s) 476
Fsp4HI GCNGC 4 cut(s) 292, 310, 508, 562
FspBI CTAG 1 cut(s) 417
GlaI GCGC 1 cut(s) 137
GluI GCNGC 4 cut(s) 292, 310, 508, 562
HaeII RGCGCY 1 cut(s) 139
HaeIII GGCC 4 cut(s) 39, 294, 309, 523
HgaI GACGC 2 cut(s) 567, 629
HhaI GCGC 1 cut(s) 138
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 2 cut(s) 64, 569
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 1 cut(s) 347
Hpy188I TCNGA 2 cut(s) 7, 58
Hpy188III TCNNGA 5 cut(s) 317, 329, 429, 597, 666
Hpy8I GTNNAC 1 cut(s) 347
Hpy99I CGWCG 1 cut(s) 645
HpyCH4III ACNGT 4 cut(s) 23, 238, 323, 548
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 1 cut(s) 404
HpyF10VI GCNNNNNNNGC 5 cut(s) 291, 513, 561, 600, 621
HpyF3I CTNAG 2 cut(s) 15, 249
HpySE526I ACGT 1 cut(s) 349
HspAI GCGC 1 cut(s) 136
KspI CCGCGG 1 cut(s) 292
Kzo9I GATC 5 cut(s) 298, 313, 353, 526, 604
LguI GCTCTTC 1 cut(s) 3
LmnI GCTCC 1 cut(s) 552
Lsp1109I GCAGC 1 cut(s) 573
MaeI CTAG 1 cut(s) 417
MaeII ACGT 1 cut(s) 349
MaeIII GTNAC 3 cut(s) 157, 317, 617
MalI GATC 5 cut(s) 300, 315, 355, 528, 606
MbiI CCGCTC 2 cut(s) 555, 624
MboI GATC 5 cut(s) 298, 313, 353, 526, 604
MboII GAAGA 2 cut(s) 20, 197
MfeI CAATTG 1 cut(s) 192
MflI RGATCY 1 cut(s) 353
MluCI AATT 1 cut(s) 192
MlyI GAGTC 2 cut(s) 73, 578
MnlI CCTC 9 cut(s) 181, 190, 264, 332, 359, 425, 428, 473, 476
MspA1I CMGCKG 2 cut(s) 291, 516
MspR9I CCNGG 2 cut(s) 46, 393
MunI CAATTG 1 cut(s) 192
MvaI CCWGG 2 cut(s) 46, 393
MvnI CGCG 2 cut(s) 291, 312
MwoI GCNNNNNNNGC 5 cut(s) 291, 513, 561, 600, 621
NdeI CATATG 1 cut(s) 634
NdeII GATC 5 cut(s) 298, 313, 353, 526, 604
NlaIV GGNNCC 1 cut(s) 522
NmuCI GTSAC 3 cut(s) 157, 317, 617
PciSI GCTCTTC 1 cut(s) 3
PcsI WCGNNNNNNNCGW 1 cut(s) 613
PkrI GCNGC 4 cut(s) 293, 311, 509, 563
Ple19I CGATCG 2 cut(s) 316, 607
PleI GAGTC 2 cut(s) 72, 577
PpsI GAGTC 2 cut(s) 72, 577
PshAI GACNNNNGTC 1 cut(s) 324
Psp6I CCWGG 2 cut(s) 44, 391
PspGI CCWGG 2 cut(s) 44, 391
PspN4I GGNNCC 1 cut(s) 522
PspPI GGNCC 2 cut(s) 521, 629
PsuI RGATCY 1 cut(s) 353
PvuI CGATCG 2 cut(s) 316, 607
RsaI GTAC 1 cut(s) 20
RsaNI GTAC 1 cut(s) 19
SacII CCGCGG 1 cut(s) 292
SapI GCTCTTC 1 cut(s) 3
SatI GCNGC 4 cut(s) 292, 310, 508, 562
Sau3AI GATC 5 cut(s) 298, 313, 353, 526, 604
Sau96I GGNCC 2 cut(s) 521, 629
SchI GAGTC 2 cut(s) 73, 578
ScrFI CCNGG 2 cut(s) 46, 393
SfcI CTRYAG 1 cut(s) 511
Sfr303I CCGCGG 1 cut(s) 292
SgrBI CCGCGG 1 cut(s) 292
SinI GGWCC 1 cut(s) 629
Sse9I AATT 1 cut(s) 192
SsiI CCGC 7 cut(s) 289, 291, 310, 508, 516, 555, 622
SspMI CTAG 1 cut(s) 417
StyD4I CCNGG 2 cut(s) 44, 391
StyI CCWWGG 2 cut(s) 108, 259
TaaI ACNGT 4 cut(s) 23, 238, 323, 548
TaiI ACGT 1 cut(s) 352
TaqI TCGA 1 cut(s) 529
TaqII GACCGA 1 cut(s) 555
TasI AATT 1 cut(s) 192
TauI GCSGC 3 cut(s) 294, 312, 510
TscAI CASTG 2 cut(s) 241, 553
TseFI GTSAC 3 cut(s) 157, 317, 617
TseI GCWGC 1 cut(s) 561
Tsp45I GTSAC 3 cut(s) 157, 317, 617
TspDTI ATGAA 1 cut(s) 218
TspGWI ACGGA 2 cut(s) 351, 650
TspRI CASTG 2 cut(s) 241, 553
VpaK11BI GGWCC 1 cut(s) 629
XagI CCTNNNNNAGG 1 cut(s) 258
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XspI CTAG 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.