Rroxscaffold_165G00436920

Glycine-rich RNA-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000165
OG0013848
Physical Location & Seq
Reverse (-)
8085 .. 11572
3488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_165G00436920.1

Sequence Viewer

Length: 1032 bp
ATGGCAAGTCGCCGTGTTGCGGGAATGCCGTGTCACAGAGTCACGAGAATGCGGAGTCGTGAGAATGCGAGTCGCGGGCAAGTAGGCCGTGTCGTGGGGGTCATGGTGTCACGAGCGAGTTGCAATGTCGTAGGTCACCGGGGTCGCGGAGTCAAGGGCGTGCGTATTGCGGTGTTGCGGTTGCAAGGGCAAGCGAGCACGCACGAGGGGAGGCGCGCGGGCTTGCGGGCGCTACGGGCGGCGAGGCTAACCCGAAAAGGCCGATACTTGTTTGATATGTTCAAGTTTTATGGTTGTATTGAGATGTATTGTACATATGAACAAAGCCCTGAATTGGTTTATAAAATGTCGGAAGAACCCGAGTATCGTTGCTTTATTGGTGGCCTTGCCTGGTCTACATCTGATAGGAGTCTAAAAGAAGCTTTTGACAAGTATGGGAAGCTTGTTGAAGCCAAGGTTGTTGTTGATAAGTTCTCGGGGCGCTCTCGTGGATTTGGATTTGTCACTTTTGATGACAAAAAAGCAATGGAAGAGGCAATTGAGGAAATGAATGGAATCGATTTAGACGGACGGACTATCACTGTTGATAAAGCTCAGCCTCACCAAGGTTCAGGTAGAGATTATGATGGTGACCGCAGCCGTGATCGTGGCGGCCGCGATCGTGACCGTGGTCGTGATTATGGAGGTGGTGGACGTGGATCTGGTACGGTAGTAGGGATGATAAGTACAGTGGAGGAGGAGGAGGTGGTGGTGGTAGCCGCTATGGAGACCGAAATGGAGGAGGAGGAGGAGGGCCTGATCGAAATGGAGACCGATACAGTGGGCGCAGCAGGGATTCTGGTTCTGGTGGTCGTTCAAGCTCTGGAAGTGATCGTTTCAGTCGTGACCGCTCTGGTCCATATGAGCGTCGGGGACAGGAGGTTTCCGTTCTGGATAGTATCAATACTTATGACAGTGCTTACGATGCAAGGTGCTGCTCAACTGGCTTTCCCCGTCTGCACTGCATTGAAGTTTCTTCTGGATATGTTCTGA

Protein Analysis

343

Amino Acids

37.97

Weight (kDa)

8.17

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 125 - 194 2.5e-21 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26420
fragaria_vesca FvH4_6g05361
malus_domestica MD04G1208800.v1.1 MD12G1223200.v1.1
prunus_persica Prupe.6G329800_v2.0.a1 Prupe.6G329800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0454141
rosa_laevigata RLG00000025479
rosa_multiflora Rmu_sc0018584.1_g000002
rosa_roxburghii Rroxscaffold_165G00436920
rosa_rugosa Rorug02G0653500 Rorug02G0653500
rosa_samantha Rh3AG056200 Rh3BG057600 Rh3CG056900 Rh3DG058100
rosa_wichuraiana Rw3G004320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 342
AccBSI CCGCTC 1 cut(s) 890
AccI GTMKAC 1 cut(s) 395
AccII CGCG 5 cut(s) 75, 147, 216, 218, 657
AclWI GGATC 1 cut(s) 706
AcoI YGGCCR 1 cut(s) 652
AfaI GTAC 3 cut(s) 313, 706, 727
AfiI CCNNNNNNNGG 4 cut(s) 19, 94, 334, 605
AgsI TTSAA 4 cut(s) 283, 449, 857, 1009
AjiI CACGTC 1 cut(s) 695
AjnI CCWGG 1 cut(s) 389
AluBI AGCT 4 cut(s) 422, 442, 593, 860
AluI AGCT 4 cut(s) 422, 442, 593, 860
Alw21I GWGCWC 1 cut(s) 200
Alw26I GTCTC 2 cut(s) 761, 803
AlwI GGATC 1 cut(s) 706
Ama87I CYCGRG 2 cut(s) 359, 475
AoxI GGCC 5 cut(s) 85, 259, 382, 652, 793
ApeKI GCWGC 3 cut(s) 636, 827, 974
AspLEI GCGC 5 cut(s) 216, 218, 232, 483, 827
AspS9I GGNCC 2 cut(s) 793, 895
AsuC2I CCSGG 1 cut(s) 140
AsuHPI GGTGA 3 cut(s) 128, 593, 641
AvaI CYCGRG 2 cut(s) 359, 475
AvaII GGWCC 1 cut(s) 895
BaeI ACNNNNGTAYC 4 cut(s) 347, 380, 696, 729
BauI CACGAG 4 cut(s) 43, 111, 203, 486
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 3 cut(s) 648, 839, 961
BccI CCATC 1 cut(s) 620
BceAI ACGGC 3 cut(s) 13, 72, 624
BcgI CGANNNNNNTGC 2 cut(s) 102, 136
BciT130I CCWGG 1 cut(s) 391
BcnI CCSGG 1 cut(s) 140
BcoDI GTCTC 2 cut(s) 761, 803
BfoI RGCGCY 2 cut(s) 233, 484
BisI GCNGC 7 cut(s) 240, 637, 652, 655, 759, 828, 975
BlpI GCTNAGC 1 cut(s) 594
BlsI GCNGC 7 cut(s) 241, 638, 653, 656, 760, 829, 976
Bme1390I CCNGG 2 cut(s) 140, 391
Bme18I GGWCC 1 cut(s) 895
BmeT110I CYCGRG 2 cut(s) 359, 475
BmgBI CACGTC 1 cut(s) 695
BmgT120I GGNCC 2 cut(s) 793, 895
BmrFI CCNGG 2 cut(s) 140, 391
BmsI GCATC 1 cut(s) 954
BoxI GACNNNNGTC 1 cut(s) 669
Bpu1102I GCTNAGC 1 cut(s) 594
BpuMI CCSGG 1 cut(s) 140
Bsa29I ATCGAT 1 cut(s) 558
BsaI GGTCTC 2 cut(s) 761, 803
BsaJI CCNNGG 4 cut(s) 139, 453, 604, 667
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 94, 334, 605
Bse1I ACTGG 1 cut(s) 987
Bse3DI GCAATG 2 cut(s) 130, 531
BseBI CCWGG 1 cut(s) 391
BseCI ATCGAT 1 cut(s) 558
BseDI CCNNGG 4 cut(s) 139, 453, 604, 667
BseGI GGATG 1 cut(s) 723
BseLI CCNNNNNNNGG 4 cut(s) 19, 94, 334, 605
BseMI GCAATG 2 cut(s) 130, 531
BseMII CTCAG 1 cut(s) 608
BseNI ACTGG 1 cut(s) 987
BsePI GCGCGC 1 cut(s) 214
BseRI GAGGAG 7 cut(s) 749, 752, 755, 794, 797, 800, 803
BseX3I CGGCCG 1 cut(s) 652
BseXI GCAGC 3 cut(s) 648, 839, 961
BsgI GTGCAG 1 cut(s) 982
Bsh1236I CGCG 5 cut(s) 75, 147, 216, 218, 657
Bsh1285I CGRYCG 2 cut(s) 655, 661
BshFI GGCC 5 cut(s) 87, 261, 384, 654, 795
BshVI ATCGAT 1 cut(s) 558
BsiEI CGRYCG 2 cut(s) 655, 661
BsiHKAI GWGCWC 1 cut(s) 200
BsiHKCI CYCGRG 2 cut(s) 359, 475
BsiSI CCGG 1 cut(s) 139
BslFI GGGAC 1 cut(s) 926
BslI CCNNNNNNNGG 4 cut(s) 19, 94, 334, 605
BsmAI GTCTC 2 cut(s) 761, 803
BsmFI GGGAC 1 cut(s) 926
BsmI GAATGC 3 cut(s) 30, 54, 70
BsnI GGCC 5 cut(s) 87, 261, 384, 654, 795
Bso31I GGTCTC 2 cut(s) 761, 803
BsoBI CYCGRG 2 cut(s) 359, 475
Bsp1286I GDGCHC 1 cut(s) 200
Bsp1407I TGTACA 1 cut(s) 311
Bsp143I GATC 5 cut(s) 643, 658, 698, 798, 870
Bsp1720I GCTNAGC 1 cut(s) 594
BspANI GGCC 5 cut(s) 87, 261, 384, 654, 795
BspCNI CTCAG 1 cut(s) 607
BspDI ATCGAT 1 cut(s) 558
BspFNI CGCG 5 cut(s) 75, 147, 216, 218, 657
BspPI GGATC 1 cut(s) 706
BspTNI GGTCTC 2 cut(s) 761, 803
BsrBI CCGCTC 1 cut(s) 890
BsrDI GCAATG 2 cut(s) 130, 531
BsrGI TGTACA 1 cut(s) 311
BsrI ACTGG 1 cut(s) 987
BssECI CCNNGG 4 cut(s) 139, 453, 604, 667
BssHII GCGCGC 1 cut(s) 214
BssMI GATC 5 cut(s) 643, 658, 698, 798, 870
BssSI CACGAG 4 cut(s) 43, 111, 203, 486
BssT1I CCWWGG 2 cut(s) 453, 604
Bst2BI CACGAG 4 cut(s) 43, 111, 203, 486
Bst2UI CCWGG 1 cut(s) 391
Bst4CI ACNGT 6 cut(s) 583, 668, 709, 730, 820, 955
Bst6I CTCTTC 1 cut(s) 525
BstAUI TGTACA 1 cut(s) 311
BstC8I GCNNGC 9 cut(s) 77, 161, 192, 196, 200, 216, 220, 224, 228
BstDEI CTNAG 1 cut(s) 594
BstDSI CCRYGG 1 cut(s) 667
BstEII GGTNACC 2 cut(s) 134, 629
BstENI CCTNNNNNAGG 1 cut(s) 603
BstF5I GGATG 1 cut(s) 723
BstFNI CGCG 5 cut(s) 75, 147, 216, 218, 657
BstH2I RGCGCY 2 cut(s) 233, 484
BstHHI GCGC 5 cut(s) 216, 218, 232, 483, 827
BstKTI GATC 5 cut(s) 646, 661, 701, 801, 873
BstMAI GTCTC 2 cut(s) 761, 803
BstMBI GATC 5 cut(s) 643, 658, 698, 798, 870
BstMCI CGRYCG 2 cut(s) 655, 661
BstMWI GCNNNNNNNGC 3 cut(s) 236, 964, 983
BstNI CCWGG 1 cut(s) 391
BstPAI GACNNNNGTC 1 cut(s) 669
BstPI GGTNACC 2 cut(s) 134, 629
BstSCI CCNGG 2 cut(s) 138, 389
BstUI CGCG 5 cut(s) 75, 147, 216, 218, 657
BstV1I GCAGC 3 cut(s) 648, 839, 961
BstX2I RGATCY 1 cut(s) 698
BstYI RGATCY 1 cut(s) 698
BstZI CGGCCG 1 cut(s) 652
Bsu15I ATCGAT 1 cut(s) 558
BsuRI GGCC 5 cut(s) 87, 261, 384, 654, 795
BsuTUI ATCGAT 1 cut(s) 558
BtgI CCRYGG 1 cut(s) 667
BtrI CACGTC 1 cut(s) 695
BtsCI GGATG 1 cut(s) 723
BtsI GCAGTG 1 cut(s) 999
BtsIMutI CAGTG 5 cut(s) 579, 735, 825, 960, 999
Cac8I GCNNGC 9 cut(s) 77, 161, 192, 196, 200, 216, 220, 224, 228
CciNI GCGGCCGC 1 cut(s) 652
CfoI GCGC 5 cut(s) 216, 218, 232, 483, 827
Cfr13I GGNCC 2 cut(s) 793, 895
ClaI ATCGAT 1 cut(s) 558
CseI GACGC 1 cut(s) 895
Csp6I GTAC 3 cut(s) 312, 705, 726
CviAII CATG 1 cut(s) 103
CviQI GTAC 3 cut(s) 312, 705, 726
DdeI CTNAG 1 cut(s) 594
DpnI GATC 5 cut(s) 645, 660, 700, 800, 872
DpnII GATC 5 cut(s) 643, 658, 698, 798, 870
EaeI YGGCCR 1 cut(s) 652
EagI CGGCCG 1 cut(s) 652
Eam1104I CTCTTC 1 cut(s) 525
EarI CTCTTC 1 cut(s) 525
EclXI CGGCCG 1 cut(s) 652
Eco130I CCWWGG 2 cut(s) 453, 604
Eco31I GGTCTC 2 cut(s) 761, 803
Eco47I GGWCC 1 cut(s) 895
Eco52I CGGCCG 1 cut(s) 652
Eco88I CYCGRG 2 cut(s) 359, 475
Eco91I GGTNACC 2 cut(s) 134, 629
EcoNI CCTNNNNNAGG 1 cut(s) 603
EcoO109I RGGNCCY 1 cut(s) 793
EcoO65I GGTNACC 2 cut(s) 134, 629
EcoRII CCWGG 1 cut(s) 389
EcoT14I CCWWGG 2 cut(s) 453, 604
ErhI CCWWGG 2 cut(s) 453, 604
FaeI CATG 1 cut(s) 106
FaqI GGGAC 1 cut(s) 926
FatI CATG 1 cut(s) 102
FauI CCCGC 4 cut(s) 13, 68, 211, 219
FauNDI CATATG 2 cut(s) 316, 900
FblI GTMKAC 1 cut(s) 395
Fnu4HI GCNGC 7 cut(s) 240, 637, 652, 655, 759, 828, 975
FokI GGATG 1 cut(s) 730
Fsp4HI GCNGC 7 cut(s) 240, 637, 652, 655, 759, 828, 975
GlaI GCGC 5 cut(s) 215, 217, 231, 482, 826
GluI GCNGC 7 cut(s) 240, 637, 652, 655, 759, 828, 975
HaeII RGCGCY 2 cut(s) 233, 484
HaeIII GGCC 5 cut(s) 87, 261, 384, 654, 795
HapII CCGG 1 cut(s) 139
HgaI GACGC 1 cut(s) 895
HhaI GCGC 5 cut(s) 216, 218, 232, 483, 827
Hin1II CATG 1 cut(s) 106
Hin6I GCGC 5 cut(s) 214, 216, 230, 481, 825
HinP1I GCGC 5 cut(s) 214, 216, 230, 481, 825
HindIII AAGCTT 2 cut(s) 420, 440
HinfI GANTC 7 cut(s) 39, 55, 70, 150, 409, 555, 835
HpaII CCGG 1 cut(s) 139
HphI GGTGA 3 cut(s) 128, 593, 641
Hpy166II GTNNAC 2 cut(s) 396, 692
Hpy188I TCNGA 3 cut(s) 352, 403, 1031
Hpy188III TCNNGA 9 cut(s) 43, 59, 111, 662, 674, 863, 883, 931, 1019
Hpy8I GTNNAC 2 cut(s) 396, 692
Hpy99I CGWCG 1 cut(s) 911
HpyCH4III ACNGT 6 cut(s) 583, 668, 709, 730, 820, 955
HpyCH4IV ACGT 1 cut(s) 694
HpyCH4V TGCA 5 cut(s) 123, 184, 967, 999, 1004
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 964, 983
HpyF3I CTNAG 1 cut(s) 594
HpySE526I ACGT 1 cut(s) 694
Hsp92II CATG 1 cut(s) 106
HspAI GCGC 5 cut(s) 214, 216, 230, 481, 825
Kzo9I GATC 5 cut(s) 643, 658, 698, 798, 870
Lsp1109I GCAGC 3 cut(s) 648, 839, 961
LweI GCATC 1 cut(s) 954
MaeII ACGT 1 cut(s) 694
MaeIII GTNAC 8 cut(s) 32, 40, 108, 134, 502, 629, 662, 883
MalI GATC 5 cut(s) 645, 660, 700, 800, 872
MbiI CCGCTC 1 cut(s) 890
MboI GATC 5 cut(s) 643, 658, 698, 798, 870
MboII GAAGA 3 cut(s) 365, 542, 1007
MfeI CAATTG 1 cut(s) 537
MflI RGATCY 1 cut(s) 698
MhlI GDGCHC 1 cut(s) 200
MluCI AATT 2 cut(s) 332, 537
MlyI GAGTC 5 cut(s) 48, 64, 79, 159, 418
MmeI TCCRAC 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 47
MspI CCGG 1 cut(s) 139
MspR9I CCNGG 2 cut(s) 140, 391
MunI CAATTG 1 cut(s) 537
Mva1269I GAATGC 3 cut(s) 30, 54, 70
MvaI CCWGG 1 cut(s) 391
MvnI CGCG 5 cut(s) 75, 147, 216, 218, 657
MwoI GCNNNNNNNGC 3 cut(s) 236, 964, 983
NciI CCSGG 1 cut(s) 140
NdeI CATATG 2 cut(s) 316, 900
NdeII GATC 5 cut(s) 643, 658, 698, 798, 870
NlaIII CATG 1 cut(s) 106
NmuCI GTSAC 8 cut(s) 32, 40, 108, 134, 502, 629, 662, 883
NotI GCGGCCGC 1 cut(s) 652
PauI GCGCGC 1 cut(s) 214
PcsI WCGNNNNNNNCGW 1 cut(s) 879
PctI GAATGC 3 cut(s) 30, 54, 70
PfeI GAWTC 2 cut(s) 555, 835
PkrI GCNGC 7 cut(s) 241, 638, 653, 656, 760, 829, 976
Ple19I CGATCG 1 cut(s) 661
PleI GAGTC 5 cut(s) 47, 63, 78, 158, 417
PpsI GAGTC 5 cut(s) 47, 63, 78, 158, 417
PshAI GACNNNNGTC 1 cut(s) 669
PsiI TTATAA 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 389
PspEI GGTNACC 2 cut(s) 134, 629
PspGI CCWGG 1 cut(s) 389
PspPI GGNCC 2 cut(s) 793, 895
PsuI RGATCY 1 cut(s) 698
PteI GCGCGC 1 cut(s) 214
PvuI CGATCG 1 cut(s) 661
RsaI GTAC 3 cut(s) 313, 706, 727
RsaNI GTAC 3 cut(s) 312, 705, 726
RseI CAYNNNNRTG 1 cut(s) 47
SatI GCNGC 7 cut(s) 240, 637, 652, 655, 759, 828, 975
Sau3AI GATC 5 cut(s) 643, 658, 698, 798, 870
Sau96I GGNCC 2 cut(s) 793, 895
SchI GAGTC 5 cut(s) 48, 64, 79, 159, 418
ScrFI CCNGG 2 cut(s) 140, 391
SduI GDGCHC 1 cut(s) 200
SfaNI GCATC 1 cut(s) 954
SinI GGWCC 1 cut(s) 895
SmiMI CAYNNNNRTG 1 cut(s) 47
Sse9I AATT 2 cut(s) 332, 537
StyD4I CCNGG 2 cut(s) 138, 389
StyI CCWWGG 2 cut(s) 453, 604
TaaI ACNGT 6 cut(s) 583, 668, 709, 730, 820, 955
TaiI ACGT 1 cut(s) 697
TaqI TCGA 2 cut(s) 558, 801
TaqII GACCGA 2 cut(s) 785, 827
TasI AATT 2 cut(s) 332, 537
TatI WGTACW 2 cut(s) 311, 725
TauI GCSGC 4 cut(s) 242, 654, 657, 761
TfiI GAWTC 2 cut(s) 555, 835
TscAI CASTG 5 cut(s) 586, 735, 825, 960, 1006
TseFI GTSAC 8 cut(s) 32, 40, 108, 134, 502, 629, 662, 883
TseI GCWGC 3 cut(s) 636, 827, 974
Tsp45I GTSAC 8 cut(s) 32, 40, 108, 134, 502, 629, 662, 883
TspDTI ATGAA 2 cut(s) 333, 563
TspGWI ACGGA 3 cut(s) 582, 586, 915
TspRI CASTG 5 cut(s) 586, 735, 825, 960, 1006
VpaK11BI GGWCC 1 cut(s) 895
XagI CCTNNNNNAGG 1 cut(s) 603
XmiI GTMKAC 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.