MD12G1223200.v1.1

Glycine-rich RNA-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013848
Physical Location & Seq
Forward (+)
29998573 .. 30001494
2922 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1223200.v1.1.491

Sequence Viewer

Length: 681 bp
ATGCTTGTGTTTGTGTGCTCGCATGGAGTTATGTCAGAAGAGCTGGAGTATCGATGCTTCATCGGTGGCCTTGCATGGTCAACATCTGATAGAAGTCTAAGAGAAGCATTTGAAAAGTTTGGAAAGCTTGTTGAAGCTAAGGTGGTGGTTGATAAGTTTTCTGGACGCTCGCGTGGATTTGGGTTTGTCACCTTTGATGATAAAAAAGCAATGGAAGAGGCCATTGAGGAAATGAATGGATTGGATTTGGATGGACGAACCATCACTGTTGATAAGGCTCAGCCTCACCAAGGTGGTGGAGGTAGAGATTTTGACAGTGACCGTGGTCGTGACCGCGGTCGTGATCGTGATCGTGGTCGCGACTTTGGTGGTGGAGGTGGACGTGGAGGTGGAGGAGACTGCTTTAAGTGTGGCAAGCCAGGACATTTTGCCAGAGAGTGTCCAAGTGAAGGTTCAAGAGGAGGGGGTGGCAGGTATGGTGGTAGGGATGATAGGTATGGTAGCAGCGGCGGCGGCGGCGGTGGTAGCGGTCGTTATGGACCTGAGCGAAATGGAGACCGACCATCTAGTGGGCGCAGCAGGGATTCTGGTAGTCGTGCAGGTTCTGGAAGTGATCGATATAGTCGTGACCGCTCTGGGCCATATGAACGTCGGAGTACAGGAGGCTTCCGTTCAGGATAG

Protein Analysis

227

Amino Acids

24.15

Weight (kDa)

9.18

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 20 - 89 6.2e-22 RNA recognition motif
zf-CCHC PF00098 134 - 149 1.1e-07 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26420
fragaria_vesca FvH4_6g05361
malus_domestica MD04G1208800.v1.1 MD12G1223200.v1.1
prunus_persica Prupe.6G329800_v2.0.a1 Prupe.6G329800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0454141
rosa_laevigata RLG00000025479
rosa_multiflora Rmu_sc0018584.1_g000002
rosa_roxburghii Rroxscaffold_165G00436920
rosa_rugosa Rorug02G0653500 Rorug02G0653500
rosa_samantha Rh3AG056200 Rh3BG057600 Rh3CG056900 Rh3DG058100
rosa_wichuraiana Rw3G004320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 462, 590
AccB7I CCANNNNNTGG 1 cut(s) 569
AccBSI CCGCTC 1 cut(s) 633
AccII CGCG 3 cut(s) 172, 336, 360
AciI CCGC 9 cut(s) 334, 336, 507, 510, 513, 516, 519, 528, 631
AfaI GTAC 1 cut(s) 658
AfiI CCNNNNNNNGG 3 cut(s) 290, 449, 569
AgsI TTSAA 3 cut(s) 113, 134, 456
AjiI CACGTC 1 cut(s) 383
AjnI CCWGG 1 cut(s) 418
AjuI GAANNNNNNNTTGG 2 cut(s) 436, 468
AleI CACNNNNGTG 1 cut(s) 291
AluBI AGCT 3 cut(s) 43, 127, 137
AluI AGCT 3 cut(s) 43, 127, 137
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 2 cut(s) 390, 549
AlwNI CAGNNNCTG 1 cut(s) 605
AoxI GGCC 3 cut(s) 67, 219, 638
ApeKI GCWGC 2 cut(s) 504, 576
AspLEI GCGC 1 cut(s) 576
AspS9I GGNCC 2 cut(s) 539, 638
AsuHPI GGTGA 2 cut(s) 181, 278
AvaII GGWCC 1 cut(s) 539
BarI GAAGNNNNNNTAC 2 cut(s) 41, 73
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 2 cut(s) 516, 588
BccI CCATC 3 cut(s) 245, 269, 571
BciT130I CCWGG 1 cut(s) 420
BcoDI GTCTC 2 cut(s) 390, 549
BfaI CTAG 1 cut(s) 567
BfuAI ACCTGC 2 cut(s) 462, 590
BisI GCNGC 6 cut(s) 505, 508, 511, 514, 517, 577
BlpI GCTNAGC 1 cut(s) 279
BlsI GCNGC 6 cut(s) 506, 509, 512, 515, 518, 578
Bme1390I CCNGG 1 cut(s) 420
Bme18I GGWCC 1 cut(s) 539
BmgBI CACGTC 1 cut(s) 383
BmgT120I GGNCC 2 cut(s) 539, 638
BmrFI CCNGG 1 cut(s) 420
BmsI GCATC 1 cut(s) 44
BoxI GACNNNNGTC 2 cut(s) 324, 336
BpmI CTGGAG 1 cut(s) 65
Bpu10I CCTNAGC 2 cut(s) 138, 543
Bpu1102I GCTNAGC 1 cut(s) 279
Bsa29I ATCGAT 2 cut(s) 52, 616
BsaBI GATNNNNATC 1 cut(s) 348
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 3 cut(s) 289, 322, 334
Bsc4I CCNNNNNNNGG 3 cut(s) 290, 449, 569
Bse3DI GCAATG 1 cut(s) 216
Bse8I GATNNNNATC 1 cut(s) 348
BseBI CCWGG 1 cut(s) 420
BseCI ATCGAT 2 cut(s) 52, 616
BseDI CCNNGG 3 cut(s) 289, 322, 334
BseGI GGATG 2 cut(s) 256, 493
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 3 cut(s) 290, 449, 569
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 2 cut(s) 293, 534
BseRI GAGGAG 2 cut(s) 408, 474
BseXI GCAGC 2 cut(s) 516, 588
BsgI GTGCAG 1 cut(s) 618
Bsh1236I CGCG 3 cut(s) 172, 336, 360
Bsh1285I CGRYCG 2 cut(s) 340, 532
BshFI GGCC 3 cut(s) 69, 221, 640
BshVI ATCGAT 2 cut(s) 52, 616
BsiEI CGRYCG 2 cut(s) 340, 532
BsiHKAI GWGCWC 1 cut(s) 20
BslI CCNNNNNNNGG 3 cut(s) 290, 449, 569
BsmAI GTCTC 2 cut(s) 390, 549
BsnI GGCC 3 cut(s) 69, 221, 640
Bso31I GGTCTC 1 cut(s) 549
Bsp1286I GDGCHC 1 cut(s) 20
Bsp143I GATC 3 cut(s) 343, 349, 613
Bsp1720I GCTNAGC 1 cut(s) 279
Bsp68I TCGCGA 1 cut(s) 360
BspACI CCGC 9 cut(s) 334, 336, 507, 510, 513, 516, 519, 528, 631
BspANI GGCC 3 cut(s) 69, 221, 640
BspCNI CTCAG 2 cut(s) 292, 535
BspDI ATCGAT 2 cut(s) 52, 616
BspFNI CGCG 3 cut(s) 172, 336, 360
BspMI ACCTGC 2 cut(s) 462, 590
BspQI GCTCTTC 1 cut(s) 33
BspTNI GGTCTC 1 cut(s) 549
BsrBI CCGCTC 1 cut(s) 633
BsrDI GCAATG 1 cut(s) 216
BssECI CCNNGG 3 cut(s) 289, 322, 334
BssMI GATC 3 cut(s) 343, 349, 613
BssT1I CCWWGG 1 cut(s) 289
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 3 cut(s) 268, 317, 323
Bst6I CTCTTC 2 cut(s) 33, 210
BstC8I GCNNGC 3 cut(s) 20, 170, 416
BstDEI CTNAG 4 cut(s) 98, 138, 279, 543
BstDSI CCRYGG 2 cut(s) 322, 334
BstENI CCTNNNNNAGG 1 cut(s) 288
BstF5I GGATG 2 cut(s) 256, 493
BstFNI CGCG 3 cut(s) 172, 336, 360
BstHHI GCGC 1 cut(s) 576
BstKTI GATC 3 cut(s) 346, 352, 616
BstMAI GTCTC 2 cut(s) 390, 549
BstMBI GATC 3 cut(s) 343, 349, 613
BstMCI CGRYCG 2 cut(s) 340, 532
BstMWI GCNNNNNNNGC 4 cut(s) 510, 513, 516, 525
BstNI CCWGG 1 cut(s) 420
BstPAI GACNNNNGTC 2 cut(s) 324, 336
BstSCI CCNGG 1 cut(s) 418
BstUI CGCG 3 cut(s) 172, 336, 360
BstV1I GCAGC 2 cut(s) 516, 588
BstXI CCANNNNNNTGG 1 cut(s) 296
Bsu15I ATCGAT 2 cut(s) 52, 616
BsuRI GGCC 3 cut(s) 69, 221, 640
BsuTUI ATCGAT 2 cut(s) 52, 616
BtgI CCRYGG 2 cut(s) 322, 334
BtrI CACGTC 1 cut(s) 383
BtsCI GGATG 2 cut(s) 256, 493
BtsIMutI CAGTG 2 cut(s) 264, 322
BtuMI TCGCGA 1 cut(s) 360
BveI ACCTGC 2 cut(s) 462, 590
Cac8I GCNNGC 3 cut(s) 20, 170, 416
CaiI CAGNNNCTG 1 cut(s) 605
CfoI GCGC 1 cut(s) 576
Cfr13I GGNCC 2 cut(s) 539, 638
Cfr42I CCGCGG 1 cut(s) 337
ClaI ATCGAT 2 cut(s) 52, 616
CseI GACGC 1 cut(s) 174
Csp6I GTAC 1 cut(s) 657
CviAII CATG 2 cut(s) 23, 75
CviQI GTAC 1 cut(s) 657
DdeI CTNAG 4 cut(s) 98, 138, 279, 543
DpnI GATC 3 cut(s) 345, 351, 615
DpnII GATC 3 cut(s) 343, 349, 613
Eam1104I CTCTTC 2 cut(s) 33, 210
EarI CTCTTC 2 cut(s) 33, 210
Eco130I CCWWGG 1 cut(s) 289
Eco31I GGTCTC 1 cut(s) 549
Eco47I GGWCC 1 cut(s) 539
EcoNI CCTNNNNNAGG 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 289
FaeI CATG 2 cut(s) 26, 78
FaiI YATR 9 cut(s) 24, 32, 76, 477, 498, 537, 621, 643, 645
FatI CATG 2 cut(s) 22, 74
FauNDI CATATG 1 cut(s) 643
Fnu4HI GCNGC 6 cut(s) 505, 508, 511, 514, 517, 577
FokI GGATG 2 cut(s) 263, 500
Fsp4HI GCNGC 6 cut(s) 505, 508, 511, 514, 517, 577
FspBI CTAG 1 cut(s) 567
GlaI GCGC 1 cut(s) 575
GluI GCNGC 6 cut(s) 505, 508, 511, 514, 517, 577
GsuI CTGGAG 1 cut(s) 65
HaeIII GGCC 3 cut(s) 69, 221, 640
HgaI GACGC 1 cut(s) 174
HhaI GCGC 1 cut(s) 576
Hin1II CATG 2 cut(s) 26, 78
Hin6I GCGC 1 cut(s) 574
HinP1I GCGC 1 cut(s) 574
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 1 cut(s) 584
HphI GGTGA 2 cut(s) 181, 278
Hpy166II GTNNAC 2 cut(s) 81, 380
Hpy188I TCNGA 3 cut(s) 37, 88, 654
Hpy188III TCNNGA 9 cut(s) 162, 329, 341, 347, 359, 456, 606, 626, 675
Hpy8I GTNNAC 2 cut(s) 81, 380
Hpy99I CGWCG 1 cut(s) 654
HpyAV CCTTC 1 cut(s) 443
HpyCH4III ACNGT 3 cut(s) 268, 317, 323
HpyCH4IV ACGT 2 cut(s) 382, 649
HpyCH4V TGCA 2 cut(s) 74, 599
HpyF10VI GCNNNNNNNGC 4 cut(s) 510, 513, 516, 525
HpyF3I CTNAG 4 cut(s) 98, 138, 279, 543
HpySE526I ACGT 2 cut(s) 382, 649
Hsp92II CATG 2 cut(s) 26, 78
HspAI GCGC 1 cut(s) 574
KspI CCGCGG 1 cut(s) 337
Kzo9I GATC 3 cut(s) 343, 349, 613
LguI GCTCTTC 1 cut(s) 33
Lsp1109I GCAGC 2 cut(s) 516, 588
LweI GCATC 1 cut(s) 44
MaeI CTAG 1 cut(s) 567
MaeII ACGT 2 cut(s) 382, 649
MaeIII GTNAC 4 cut(s) 187, 317, 329, 626
MalI GATC 3 cut(s) 345, 351, 615
MbiI CCGCTC 1 cut(s) 633
MboI GATC 3 cut(s) 343, 349, 613
MboII GAAGA 2 cut(s) 50, 227
MhlI GDGCHC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 632
MseI TTAA 1 cut(s) 405
MslI CAYNNNNRTG 1 cut(s) 291
MspA1I CMGCKG 2 cut(s) 336, 507
MspR9I CCNGG 1 cut(s) 420
MvaI CCWGG 1 cut(s) 420
MvnI CGCG 3 cut(s) 172, 336, 360
MwoI GCNNNNNNNGC 4 cut(s) 510, 513, 516, 525
NdeI CATATG 1 cut(s) 643
NdeII GATC 3 cut(s) 343, 349, 613
NlaIII CATG 2 cut(s) 26, 78
NmuCI GTSAC 4 cut(s) 187, 317, 329, 626
NruI TCGCGA 1 cut(s) 360
OliI CACNNNNGTG 1 cut(s) 291
PciSI GCTCTTC 1 cut(s) 33
PcsI WCGNNNNNNNCGW 1 cut(s) 622
PfeI GAWTC 1 cut(s) 584
PflMI CCANNNNNTGG 1 cut(s) 569
PkrI GCNGC 6 cut(s) 506, 509, 512, 515, 518, 578
PshAI GACNNNNGTC 2 cut(s) 324, 336
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PspPI GGNCC 2 cut(s) 539, 638
PstNI CAGNNNCTG 1 cut(s) 605
RruI TCGCGA 1 cut(s) 360
RsaI GTAC 1 cut(s) 658
RsaNI GTAC 1 cut(s) 657
RseI CAYNNNNRTG 1 cut(s) 291
SacII CCGCGG 1 cut(s) 337
SapI GCTCTTC 1 cut(s) 33
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 6 cut(s) 505, 508, 511, 514, 517, 577
Sau3AI GATC 3 cut(s) 343, 349, 613
Sau96I GGNCC 2 cut(s) 539, 638
ScrFI CCNGG 1 cut(s) 420
SduI GDGCHC 1 cut(s) 20
SfaNI GCATC 1 cut(s) 44
Sfr303I CCGCGG 1 cut(s) 337
SgrBI CCGCGG 1 cut(s) 337
SinI GGWCC 1 cut(s) 539
SmiMI CAYNNNNRTG 1 cut(s) 291
SsiI CCGC 9 cut(s) 334, 336, 507, 510, 513, 516, 519, 528, 631
SspMI CTAG 1 cut(s) 567
StyD4I CCNGG 1 cut(s) 418
StyI CCWWGG 1 cut(s) 289
TaaI ACNGT 3 cut(s) 268, 317, 323
TaiI ACGT 2 cut(s) 385, 652
TaqI TCGA 2 cut(s) 52, 616
TaqII GACCGA 1 cut(s) 573
TatI WGTACW 1 cut(s) 656
TauI GCSGC 4 cut(s) 510, 513, 516, 519
TfiI GAWTC 1 cut(s) 584
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 2 cut(s) 271, 322
TseFI GTSAC 4 cut(s) 187, 317, 329, 626
TseI GCWGC 2 cut(s) 504, 576
Tsp45I GTSAC 4 cut(s) 187, 317, 329, 626
TspDTI ATGAA 3 cut(s) 49, 248, 660
TspGWI ACGGA 1 cut(s) 659
TspRI CASTG 2 cut(s) 271, 322
Van91I CCANNNNNTGG 1 cut(s) 569
VpaK11BI GGWCC 1 cut(s) 539
XagI CCTNNNNNAGG 1 cut(s) 288
XspI CTAG 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.