MD04G1208800.v1.1

Glycine-rich RNA-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013848
Physical Location & Seq
Forward (+)
29407302 .. 29410228
2927 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1208800.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGGGATGTCAGAAGAGCTGGAGTATCGATGCTTCATCGGTGGCCTTGCATGGTCAACATCTGATAGAAGTCTAAGAGAAGCATTTGAAAAGTTTGGAAAGCTCGTTGAAGCTAAGGTGGTTGTTGATAAATTTTCTGGACGCTCTCGTGGATTTGGGTTTGTGACTTTTGATGATAAAAAAGCAATGGAAGAGGCCATTGAGGAAATGAATGGATTGGATTTAGATGGACGAACTATCACTGTTGATAAAGCTCAGCCTCACCAAGGTGGTGGAGGTAGAGATTTTGATGGTGACCGTGGTGGTGACCGCGGTCGTGATCGTGACCGTGGTCGCGACTTTGGAGGTGGACGTGGACGTGGAGGTGGAGGTGGAGGAGACTGCTTCAAGTGTGGCAAGCCAGGGCATTTTGCCAGGGAGTGTCCAAGTGAAGGTTCAAGAGGAGGGGGTGGCAGGTATGGTGATAGGGATGATAAGTATGGTGGCGGTGGTGGTGGTAGCGGTCGTTATGGACCTGATCGAAATGGAGACCGTTCATCTAGTGGGCGCAGCAGGGATTCAGGTAGTCGTGGAGGTTCTGGAAGTGATCGGTACAGTCGTGACCGCTCTGGCCCATATGAACGTCGGAGTACAGGTGGCTTCCGTTCTGGATAG

Protein Analysis

218

Amino Acids

23.11

Weight (kDa)

9.09

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 12 - 81 5.7e-22 RNA recognition motif
zf-CCHC PF00098 128 - 143 1.1e-07 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26420
fragaria_vesca FvH4_6g05361
malus_domestica MD04G1208800.v1.1 MD12G1223200.v1.1
prunus_persica Prupe.6G329800_v2.0.a1 Prupe.6G329800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0454141
rosa_laevigata RLG00000025479
rosa_multiflora Rmu_sc0018584.1_g000002
rosa_roxburghii Rroxscaffold_165G00436920
rosa_rugosa Rorug02G0653500 Rorug02G0653500
rosa_samantha Rh3AG056200 Rh3BG057600 Rh3CG056900 Rh3DG058100
rosa_wichuraiana Rw3G004320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 444
AccBSI CCGCTC 1 cut(s) 606
AccII CGCG 2 cut(s) 312, 336
AciI CCGC 5 cut(s) 310, 312, 486, 501, 604
AcsI RAATTY 1 cut(s) 131
AfaI GTAC 2 cut(s) 593, 631
AfiI CCNNNNNNNGG 2 cut(s) 266, 431
AgsI TTSAA 4 cut(s) 89, 110, 388, 438
AjiI CACGTC 2 cut(s) 353, 359
AjnI CCWGG 2 cut(s) 400, 413
AjuI GAANNNNNNNTTGG 2 cut(s) 418, 450
AleI CACNNNNGTG 1 cut(s) 267
AluBI AGCT 4 cut(s) 19, 103, 113, 254
AluI AGCT 4 cut(s) 19, 103, 113, 254
Alw26I GTCTC 2 cut(s) 372, 522
AoxI GGCC 3 cut(s) 43, 195, 610
ApeKI GCWGC 1 cut(s) 549
ApoI RAATTY 1 cut(s) 131
AspLEI GCGC 1 cut(s) 549
AspS9I GGNCC 2 cut(s) 512, 611
AsuHPI GGTGA 4 cut(s) 254, 305, 317, 473
AvaII GGWCC 1 cut(s) 512
BaeI ACNNNNGTAYC 2 cut(s) 583, 616
BarI GAAGNNNNNNTAC 2 cut(s) 17, 49
BauI CACGAG 1 cut(s) 147
BbvI GCAGC 1 cut(s) 561
BccI CCATC 2 cut(s) 221, 284
BciT130I CCWGG 2 cut(s) 402, 415
BcoDI GTCTC 2 cut(s) 372, 522
BfaI CTAG 1 cut(s) 540
BfuAI ACCTGC 1 cut(s) 444
BisI GCNGC 1 cut(s) 550
BlpI GCTNAGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 551
Bme1390I CCNGG 2 cut(s) 402, 415
Bme18I GGWCC 1 cut(s) 512
BmgBI CACGTC 2 cut(s) 353, 359
BmgT120I GGNCC 2 cut(s) 512, 611
BmrFI CCNGG 2 cut(s) 402, 415
BmsI GCATC 1 cut(s) 20
BoxI GACNNNNGTC 2 cut(s) 312, 330
BpmI CTGGAG 1 cut(s) 41
Bpu10I CCTNAGC 1 cut(s) 114
Bpu1102I GCTNAGC 1 cut(s) 255
Bsa29I ATCGAT 1 cut(s) 28
BsaI GGTCTC 1 cut(s) 522
BsaJI CCNNGG 6 cut(s) 265, 298, 310, 328, 401, 414
BsaXI ACNNNNNCTCC 2 cut(s) 619, 649
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 431
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 2 cut(s) 402, 415
BseCI ATCGAT 1 cut(s) 28
BseDI CCNNGG 6 cut(s) 265, 298, 310, 328, 401, 414
BseGI GGATG 2 cut(s) 12, 475
BseLI CCNNNNNNNGG 2 cut(s) 266, 431
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 1 cut(s) 269
BseRI GAGGAG 2 cut(s) 390, 456
BseXI GCAGC 1 cut(s) 561
Bsh1236I CGCG 2 cut(s) 312, 336
Bsh1285I CGRYCG 2 cut(s) 316, 505
BshFI GGCC 3 cut(s) 45, 197, 612
BshVI ATCGAT 1 cut(s) 28
BsiEI CGRYCG 2 cut(s) 316, 505
BslI CCNNNNNNNGG 2 cut(s) 266, 431
BsmAI GTCTC 2 cut(s) 372, 522
BsnI GGCC 3 cut(s) 45, 197, 612
Bso31I GGTCTC 1 cut(s) 522
Bsp143I GATC 3 cut(s) 319, 517, 586
Bsp1720I GCTNAGC 1 cut(s) 255
Bsp68I TCGCGA 1 cut(s) 336
BspACI CCGC 5 cut(s) 310, 312, 486, 501, 604
BspANI GGCC 3 cut(s) 45, 197, 612
BspCNI CTCAG 1 cut(s) 268
BspDI ATCGAT 1 cut(s) 28
BspFNI CGCG 2 cut(s) 312, 336
BspMI ACCTGC 1 cut(s) 444
BspQI GCTCTTC 1 cut(s) 9
BspTNI GGTCTC 1 cut(s) 522
BsrBI CCGCTC 1 cut(s) 606
BsrDI GCAATG 1 cut(s) 192
BssECI CCNNGG 6 cut(s) 265, 298, 310, 328, 401, 414
BssMI GATC 3 cut(s) 319, 517, 586
BssSI CACGAG 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 147
Bst2UI CCWGG 2 cut(s) 402, 415
Bst4CI ACNGT 5 cut(s) 244, 299, 329, 533, 596
Bst6I CTCTTC 2 cut(s) 9, 186
BstC8I GCNNGC 1 cut(s) 398
BstDEI CTNAG 3 cut(s) 74, 114, 255
BstDSI CCRYGG 3 cut(s) 298, 310, 328
BstEII GGTNACC 2 cut(s) 293, 305
BstENI CCTNNNNNAGG 1 cut(s) 264
BstF5I GGATG 2 cut(s) 12, 475
BstFNI CGCG 2 cut(s) 312, 336
BstHHI GCGC 1 cut(s) 549
BstKTI GATC 3 cut(s) 322, 520, 589
BstMAI GTCTC 2 cut(s) 372, 522
BstMBI GATC 3 cut(s) 319, 517, 586
BstMCI CGRYCG 2 cut(s) 316, 505
BstNI CCWGG 2 cut(s) 402, 415
BstPAI GACNNNNGTC 2 cut(s) 312, 330
BstPI GGTNACC 2 cut(s) 293, 305
BstSCI CCNGG 2 cut(s) 400, 413
BstUI CGCG 2 cut(s) 312, 336
BstV1I GCAGC 1 cut(s) 561
BstXI CCANNNNNNTGG 1 cut(s) 272
Bsu15I ATCGAT 1 cut(s) 28
BsuRI GGCC 3 cut(s) 45, 197, 612
BsuTUI ATCGAT 1 cut(s) 28
BtgI CCRYGG 3 cut(s) 298, 310, 328
BtrI CACGTC 2 cut(s) 353, 359
BtsCI GGATG 2 cut(s) 12, 475
BtsIMutI CAGTG 1 cut(s) 240
BtuMI TCGCGA 1 cut(s) 336
BveI ACCTGC 1 cut(s) 444
Cac8I GCNNGC 1 cut(s) 398
CfoI GCGC 1 cut(s) 549
Cfr13I GGNCC 2 cut(s) 512, 611
Cfr42I CCGCGG 1 cut(s) 313
ClaI ATCGAT 1 cut(s) 28
CseI GACGC 1 cut(s) 150
Csp6I GTAC 2 cut(s) 592, 630
CviAII CATG 1 cut(s) 51
CviQI GTAC 2 cut(s) 592, 630
DdeI CTNAG 3 cut(s) 74, 114, 255
DpnI GATC 3 cut(s) 321, 519, 588
DpnII GATC 3 cut(s) 319, 517, 586
Eam1104I CTCTTC 2 cut(s) 9, 186
EarI CTCTTC 2 cut(s) 9, 186
Eco130I CCWWGG 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 522
Eco47I GGWCC 1 cut(s) 512
Eco91I GGTNACC 2 cut(s) 293, 305
EcoNI CCTNNNNNAGG 1 cut(s) 264
EcoO65I GGTNACC 2 cut(s) 293, 305
EcoRII CCWGG 2 cut(s) 400, 413
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 1 cut(s) 54
FaiI YATR 6 cut(s) 52, 459, 480, 510, 616, 618
FatI CATG 1 cut(s) 50
FauNDI CATATG 1 cut(s) 616
Fnu4HI GCNGC 1 cut(s) 550
FokI GGATG 2 cut(s) 19, 482
Fsp4HI GCNGC 1 cut(s) 550
FspBI CTAG 1 cut(s) 540
GlaI GCGC 1 cut(s) 548
GluI GCNGC 1 cut(s) 550
GsuI CTGGAG 1 cut(s) 41
HaeIII GGCC 3 cut(s) 45, 197, 612
HgaI GACGC 1 cut(s) 150
HhaI GCGC 1 cut(s) 549
Hin1II CATG 1 cut(s) 54
Hin6I GCGC 1 cut(s) 547
HinP1I GCGC 1 cut(s) 547
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HinfI GANTC 1 cut(s) 557
HphI GGTGA 4 cut(s) 254, 305, 317, 473
Hpy166II GTNNAC 3 cut(s) 57, 350, 356
Hpy188I TCNGA 3 cut(s) 13, 64, 627
Hpy188III TCNNGA 8 cut(s) 138, 317, 323, 335, 438, 579, 599, 648
Hpy8I GTNNAC 3 cut(s) 57, 350, 356
Hpy99I CGWCG 1 cut(s) 627
HpyAV CCTTC 1 cut(s) 425
HpyCH4III ACNGT 5 cut(s) 244, 299, 329, 533, 596
HpyCH4IV ACGT 3 cut(s) 352, 358, 622
HpyCH4V TGCA 1 cut(s) 50
HpyF3I CTNAG 3 cut(s) 74, 114, 255
HpySE526I ACGT 3 cut(s) 352, 358, 622
Hsp92II CATG 1 cut(s) 54
HspAI GCGC 1 cut(s) 547
KspI CCGCGG 1 cut(s) 313
Kzo9I GATC 3 cut(s) 319, 517, 586
LguI GCTCTTC 1 cut(s) 9
Lsp1109I GCAGC 1 cut(s) 561
LweI GCATC 1 cut(s) 20
MaeI CTAG 1 cut(s) 540
MaeII ACGT 3 cut(s) 352, 358, 622
MaeIII GTNAC 5 cut(s) 163, 293, 305, 323, 599
MalI GATC 3 cut(s) 321, 519, 588
MbiI CCGCTC 1 cut(s) 606
MboI GATC 3 cut(s) 319, 517, 586
MboII GAAGA 2 cut(s) 26, 203
MluCI AATT 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 605
MslI CAYNNNNRTG 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 312
MspR9I CCNGG 2 cut(s) 402, 415
MvaI CCWGG 2 cut(s) 402, 415
MvnI CGCG 2 cut(s) 312, 336
NdeI CATATG 1 cut(s) 616
NdeII GATC 3 cut(s) 319, 517, 586
NlaIII CATG 1 cut(s) 54
NmuCI GTSAC 5 cut(s) 163, 293, 305, 323, 599
NruI TCGCGA 1 cut(s) 336
OliI CACNNNNGTG 1 cut(s) 267
PciSI GCTCTTC 1 cut(s) 9
PcsI WCGNNNNNNNCGW 1 cut(s) 595
PfeI GAWTC 1 cut(s) 557
PkrI GCNGC 1 cut(s) 551
PshAI GACNNNNGTC 2 cut(s) 312, 330
Psp6I CCWGG 2 cut(s) 400, 413
PspEI GGTNACC 2 cut(s) 293, 305
PspGI CCWGG 2 cut(s) 400, 413
PspPI GGNCC 2 cut(s) 512, 611
RruI TCGCGA 1 cut(s) 336
RsaI GTAC 2 cut(s) 593, 631
RsaNI GTAC 2 cut(s) 592, 630
RseI CAYNNNNRTG 1 cut(s) 267
SacII CCGCGG 1 cut(s) 313
SapI GCTCTTC 1 cut(s) 9
SatI GCNGC 1 cut(s) 550
Sau3AI GATC 3 cut(s) 319, 517, 586
Sau96I GGNCC 2 cut(s) 512, 611
ScrFI CCNGG 2 cut(s) 402, 415
SfaNI GCATC 1 cut(s) 20
Sfr303I CCGCGG 1 cut(s) 313
SgrBI CCGCGG 1 cut(s) 313
SinI GGWCC 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 267
Sse9I AATT 1 cut(s) 131
SsiI CCGC 5 cut(s) 310, 312, 486, 501, 604
SspMI CTAG 1 cut(s) 540
StyD4I CCNGG 2 cut(s) 400, 413
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 5 cut(s) 244, 299, 329, 533, 596
TaiI ACGT 3 cut(s) 355, 361, 625
TaqI TCGA 2 cut(s) 28, 520
TasI AATT 1 cut(s) 131
TatI WGTACW 1 cut(s) 629
TfiI GAWTC 1 cut(s) 557
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 5 cut(s) 163, 293, 305, 323, 599
TseI GCWGC 1 cut(s) 549
Tsp45I GTSAC 5 cut(s) 163, 293, 305, 323, 599
TspDTI ATGAA 4 cut(s) 25, 224, 525, 633
TspGWI ACGGA 1 cut(s) 632
TspRI CASTG 1 cut(s) 247
VpaK11BI GGWCC 1 cut(s) 512
XagI CCTNNNNNAGG 1 cut(s) 264
XapI RAATTY 1 cut(s) 131
XspI CTAG 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.