Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0007134

Physical Location & Seq

Genomic Coordinates Forward (+)

3627875 .. 3634721

Gene Structure 6847 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g06301.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2019 bp

ATGGCTACTATGGGTCGGGCGAGCTCGGGGCTCCAATACCCTGAGCGGTTCTACGCGGCGGCCTCCTACGCCGGCTTCGACGGATCTCCTCGCTCCTCCTCCAAAGCCGTTCGCGCCAAGTTCAACTCCGAGTCCGCTCTCATTCTCTACGCTCTCTATCAGCAGGCTACTGTAGGACCGTGTAACACACCGGAGCCGAGTGCTTGGAATGCAGTTGAGAAGAGCAAATGGAGCAGCTGGAAGAAGCTTGCGGACATGGCCTCCACTGAAGCAATGCGTCTTTTTGTAAAGATATTGGAGGAAGAAGATCCAGGTTGGTATTCAAGAGCATCAAACTCTGTTTCTGAGCCTGTGGTAGACGTACAAATGAACCAAAATTCAAAAGGTGGGCCAGTTGTTGAGAATGGGAACACTTATCCAGAGACAAAGACAATCTCCACTGAAAATGGGAGCATACAGGAAGCACATGATAAAGATGTTGTCTCCGAAGGCATTGGCTCTGTTGGTGTTTATGATAAGTGGATTGCTCCTCCAGTATCTGGCCAACATCCAAAAGCCCGATATGAGCATGGAGCAGCAGTTATACAAGACAAGATGTATATATATGGTGGAAACCACAATGGTCGTTACCTAAATGATCTCCATGTGTTCGATTTGAGGAGTTGGACTTGGTCAAAGATTGAGGCCAAAGTTGGGGATGAATCACATGGCTCACCATCTCCAGTAACAGTAAGCCCATGTGCTGGTCATTCCTTGATACCATGGGATAATAAACTTCTCTCAGTTGCTGGACATACAAAGGATCCTACTGAGTCTATTCAAGTGAAGGCGTTTGATCTACACACTTCTTCTTGGTCATTCTTAAAGACTTATGGGAAACCACCGACGTCACGCGGAGGTCAATCCGTCACCCTTATTGGAGGTAGCTTGGTGGTATTTGGTGGACAGGATGCGAAGAGAACTTTGTTAAATGATCTCCACATTCTTGACCTAGAAACAATGACATGGGATGAAATTGATGCTGTAGGGGCGTCCCCTTCTCCAAGGTCGGATCATGCTGCTGCTGTTCATGCGGACCGCTACCTCCTTATCTTTGGTGGGGGTTCCCATGCAACTTGCTTCAATGATCTGCATGTCCTTGATTTGCAAACTATGGAGTGGTCAAAACCCACACAACAAGGTGAGATACCAACTCCACGGGCTGGGCATGCAGGTGTGCCAGTTGGTGAAAACTGGTTCATTGTTGGGGGTGGTGACAATAAGAGTGGGGTTTCAGAAACCGTGGTCCTAAATATGTCTACACTAGTTTGGTCTGTTGTAACTACTGTTGAAGGTCGTATTCCTGTTGCTAGTGAGGGATTAAGTTTGGTCGTAGGTTGTTACAATGGTGAAGATGTGCTTTTATCTTTTGGAGGATACAATGGCCGTTACAACAATGAGGTTAACGTTCTCAAACCAAGCCTCAAGTCAACCTTGCAATCAAAAACTGAAACTTTGGTGCCAGACAGTGTTTCTGCAGTGCATGATGCCCCTAATGTTGCCAGAGATGTGCAGTCCGAGTATGAAGCCGGACAAGATGGAAAAATTAGGGAAATAGTTATGGACGCAGAGCCGATGAAATCCAGAGGCAAGAATTTTAATGAGCATCTTGTGGCGACCCTCAAAGCAGATAAAGAAGAATTAGAATCATCACTCAGTAAGGAGAAGGCACAATCTCTCCAGTTAAAGCAAGAGTTATCAGAAGCAGAGACTCGTAACACTGACCTATACAAGGAGCTTCAATCTGTACGGGGACAACTTGCATCCGAGCAATCCAGATGCTTCAAACTGGAGGTCGAACTAGCGGAACTACGACAGAAGCTCCAAAATATGGAGGCATTGCAGAAGGAACTTGAACTCCTCCAGCGACAAAAAGCTGCATCTGAACAAGCAGCCCTGAATGCTAGAAAGCAGGGCTCAGGAGGCGTATGGGGCTGGCTTGCCGGAACACCTGGCAGCGAAAAATCTGATGATGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000062 GO:0000166 GO:0001101 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006810 GO:0006869 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009753 GO:0010033 GO:0010876 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033218 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048037 GO:0050662 GO:0050896 GO:0051179 GO:0051234 GO:0071702 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901567 GO:1901681 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ACBP Kelch_3 Kelch_4 Kelch_5 Kelch_6

Protein Analysis

673

Amino Acids

73.1

Weight (kDa)

5.45

Isoelectric Point (pI)

43.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACBP	PF00887	17 - 98	7.3e-19	Acyl CoA binding protein
Beta-prop_FBX42	PF13415	171 - 230	1.7e-12	FBX42, beta-propeller domain
Kelch_HCF	PF13854	172 - 367	1.8e-19	Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	173 - 230	9.1e-17	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1	PF24981	173 - 303	6.5e-19	Attractin/LZTR1 beta-propeller
Beta-prop_TYW4	PF13418	182 - 374	2.1e-06	tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_1	PF01344	186 - 227	2.6e-10	Kelch motif
Beta-prop_FBX42	PF13415	245 - 368	3.6e-24	FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	249 - 472	2e-56	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1	PF24981	262 - 394	1.2e-17	Attractin/LZTR1 beta-propeller
Beta-prop_ATRN-LZTR1	PF24981	311 - 486	1.5e-21	Attractin/LZTR1 beta-propeller
Beta-prop_FBX42	PF13415	352 - 501	3.7e-21	FBX42, beta-propeller domain
Kelch_HCF	PF13854	361 - 417	1.6e-07	Host cell factor, Kelch-repeats domain
Kelch_HCF	PF13854	393 - 487	9.8e-06	Host cell factor, Kelch-repeats domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007134)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05420 AT3G05420 AT5G27630 AT5G27630 AT5G27630 AT5G27630
fragaria_vesca	FvH4_6g06301 FvH4_6g06301 FvH4_6g06301
malus_domestica	MD04G1202500.v1.1 MD12G1216500.v1.1
prunus_persica	Prupe.6G322800_v2.0.a1 Prupe.6G322800_v2.0.a1
pyrus_communis	pycom04g17870 pycom12g20200
rosa_chinensis	RchiOBHm_Chr3g0455341
rosa_laevigata	RLG00000025368
rosa_multiflora	Rmu_sc0002169.1_g000045 Rmu_sc0024960.1_g000003
rosa_roxburghii	Rroxscaffold_6G00424350
rosa_rugosa	Rorug03G0006600 Rorug03G0006700
rosa_samantha	Rh3CG066800 Rh3DG068300
rosa_wichuraiana	Rw3G005220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1202
AatII	GACGTC	1 cut(s)	890
Acc36I	ACCTGC	1 cut(s)	1202
AccB1I	GGYRCC	1 cut(s)	1498
AccB7I	CCANNNNNTGG	4 cut(s)	539, 743, 1202, 1870
AccBSI	CCGCTC	2 cut(s)	46, 137
AccI	GTMKAC	2 cut(s)	357, 1298
AccII	CGCG	3 cut(s)	56, 114, 894
AciI	CCGC	9 cut(s)	46, 56, 59, 135, 251, 894, 1073, 1078, 1844
AclI	AACGTT	1 cut(s)	1446
AclWI	GGATC	5 cut(s)	91, 302, 797, 810, 1059
AcoI	YGGCCR	2 cut(s)	541, 1423
AcsI	RAATTY	2 cut(s)	376, 1633
AcuI	CTGAAG	1 cut(s)	288
AcyI	GRCGYC	2 cut(s)	887, 1031
AfaI	GTAC	2 cut(s)	363, 1788
AfiI	CCNNNNNNNGG	6 cut(s)	539, 693, 743, 1202, 1771, 1870
AgsI	TTSAA	9 cut(s)	124, 324, 381, 821, 1123, 1331, 1781, 1825, 1895
AhlI	ACTAGT	1 cut(s)	1303
AjnI	CCWGG	2 cut(s)	310, 1990
AloI	GAACNNNNNNTCC	2 cut(s)	1881, 1913
AluBI	AGCT	7 cut(s)	24, 237, 247, 927, 1777, 1861, 1916
AluI	AGCT	7 cut(s)	24, 237, 247, 927, 1777, 1861, 1916
Alw21I	GWGCWC	1 cut(s)	26
Alw26I	GTCTC	3 cut(s)	416, 487, 1742
AlwI	GGATC	5 cut(s)	91, 302, 797, 810, 1059
AlwNI	CAGNNNCTG	2 cut(s)	539, 788
Ama87I	CYCGRG	1 cut(s)	25
AoxI	GGCC	6 cut(s)	60, 258, 389, 541, 684, 1423
ApeKI	GCWGC	7 cut(s)	234, 575, 1058, 1061, 1916, 1931, 1995
ApoI	RAATTY	2 cut(s)	376, 1633
ArsI	GACNNNNNNTTYG	2 cut(s)	1818, 1850
AspLEI	GCGC	1 cut(s)	116
AspS9I	GGNCC	4 cut(s)	176, 389, 1075, 1285
AsuHPI	GGTGA	6 cut(s)	705, 901, 1193, 1238, 1265, 1400
AvaI	CYCGRG	1 cut(s)	25
AvaII	GGWCC	3 cut(s)	176, 1075, 1285
BalI	TGGCCA	1 cut(s)	543
BamHI	GGATCC	1 cut(s)	802
BanI	GGYRCC	1 cut(s)	1498
BanII	GRGCYC	3 cut(s)	26, 33, 1958
Bbv12I	GWGCWC	1 cut(s)	26
BbvI	GCAGC	7 cut(s)	246, 587, 1045, 1048, 1903, 1943, 2007
BccI	CCATC	2 cut(s)	724, 1571
BceAI	ACGGC	2 cut(s)	92, 1410
BciT130I	CCWGG	2 cut(s)	312, 1992
BciVI	GTATCC	1 cut(s)	1409
BcoDI	GTCTC	3 cut(s)	416, 487, 1742
BcuI	ACTAGT	1 cut(s)	1303
BfaI	CTAG	5 cut(s)	992, 1304, 1350, 1841, 1944
BfmI	CTRYAG	3 cut(s)	171, 1023, 1515
BfuAI	ACCTGC	1 cut(s)	1202
BfuI	GTATCC	1 cut(s)	1409
BisI	GCNGC	9 cut(s)	57, 60, 235, 576, 1059, 1062, 1917, 1932, 1996
BlsI	GCNGC	9 cut(s)	58, 61, 236, 577, 1060, 1063, 1918, 1933, 1997
Bme1390I	CCNGG	2 cut(s)	312, 1992
Bme18I	GGWCC	3 cut(s)	176, 1075, 1285
BmeT110I	CYCGRG	1 cut(s)	25
BmgT120I	GGNCC	4 cut(s)	176, 389, 1075, 1285
BmiI	GGNNCC	5 cut(s)	32, 195, 804, 1105, 1500
BmrFI	CCNGG	2 cut(s)	312, 1992
BmsI	GCATC	9 cut(s)	338, 940, 1009, 1516, 1654, 1808, 1811, 1928, 2002
BpmI	CTGGAG	5 cut(s)	516, 705, 1703, 1850, 1886
Bpu10I	CCTNAGC	2 cut(s)	42, 1957
BpuEI	CTTGAG	1 cut(s)	1448
BsaHI	GRCGYC	2 cut(s)	887, 1031
BsaJI	CCNNGG	4 cut(s)	761, 1043, 1196, 1281
BsaWI	WCCGGW	1 cut(s)	190
Bsc4I	CCNNNNNNNGG	6 cut(s)	539, 693, 743, 1202, 1771, 1870
Bse118I	RCCGGY	1 cut(s)	71
Bse1I	ACTGG	7 cut(s)	392, 533, 722, 1220, 1238, 1720, 1833
Bse3DI	GCAATG	2 cut(s)	279, 1877
BseBI	CCWGG	2 cut(s)	312, 1992
BseDI	CCNNGG	4 cut(s)	761, 1043, 1196, 1281
BseGI	GGATG	5 cut(s)	547, 703, 955, 1015, 1802
BseLI	CCNNNNNNNGG	6 cut(s)	539, 693, 743, 1202, 1771, 1870
BseMI	GCAATG	2 cut(s)	279, 1877
BseMII	CTCAG	6 cut(s)	33, 336, 795, 801, 1708, 1971
BseNI	ACTGG	7 cut(s)	392, 533, 722, 1220, 1238, 1720, 1833
BseRI	GAGGAG	6 cut(s)	78, 85, 88, 519, 673, 1889
BseXI	GCAGC	7 cut(s)	246, 587, 1045, 1048, 1903, 1943, 2007
BseYI	CCCAGC	1 cut(s)	1202
BsgI	GTGCAG	1 cut(s)	1571
Bsh1236I	CGCG	3 cut(s)	56, 114, 894
BshFI	GGCC	6 cut(s)	62, 260, 391, 543, 686, 1425
BshNI	GGYRCC	1 cut(s)	1498
BsiHKAI	GWGCWC	1 cut(s)	26
BsiHKCI	CYCGRG	1 cut(s)	25
BsiSI	CCGG	4 cut(s)	72, 191, 1569, 1983
BslFI	GGGAC	2 cut(s)	1018, 1806
BslI	CCNNNNNNNGG	6 cut(s)	539, 693, 743, 1202, 1771, 1870
BsmAI	GTCTC	3 cut(s)	416, 487, 1742
BsmFI	GGGAC	2 cut(s)	1018, 1806
BsmI	GAATGC	2 cut(s)	214, 1945
BsnI	GGCC	6 cut(s)	62, 260, 391, 543, 686, 1425
BsoBI	CYCGRG	1 cut(s)	25
Bsp1286I	GDGCHC	3 cut(s)	26, 33, 1958
Bsp143I	GATC	8 cut(s)	83, 307, 637, 802, 835, 973, 1051, 1126
Bsp19I	CCATGG	1 cut(s)	761
BspACI	CCGC	9 cut(s)	46, 56, 59, 135, 251, 894, 1073, 1078, 1844
BspANI	GGCC	6 cut(s)	62, 260, 391, 543, 686, 1425
BspCNI	CTCAG	6 cut(s)	34, 337, 794, 802, 1707, 1970
BspFNI	CGCG	3 cut(s)	56, 114, 894
BspLI	GGNNCC	5 cut(s)	32, 195, 804, 1105, 1500
BspMAI	CTGCAG	1 cut(s)	1519
BspMI	ACCTGC	1 cut(s)	1202
BspPI	GGATC	5 cut(s)	91, 302, 797, 810, 1059
BspQI	GCTCTTC	1 cut(s)	215
BspT107I	GGYRCC	1 cut(s)	1498
BsrBI	CCGCTC	2 cut(s)	46, 137
BsrDI	GCAATG	2 cut(s)	279, 1877
BsrFI	RCCGGY	1 cut(s)	71
BsrI	ACTGG	7 cut(s)	392, 533, 722, 1220, 1238, 1720, 1833
BssAI	RCCGGY	1 cut(s)	71
BssECI	CCNNGG	4 cut(s)	761, 1043, 1196, 1281
BssMI	GATC	8 cut(s)	83, 307, 637, 802, 835, 973, 1051, 1126
BssNI	GRCGYC	2 cut(s)	887, 1031
BssT1I	CCWWGG	2 cut(s)	761, 1043
Bst2UI	CCWGG	2 cut(s)	312, 1992
Bst4CI	ACNGT	6 cut(s)	172, 180, 730, 1282, 1327, 1508
Bst6I	CTCTTC	2 cut(s)	215, 950
BstACI	GRCGYC	2 cut(s)	887, 1031
BstC8I	GCNNGC	7 cut(s)	22, 73, 165, 249, 1209, 1976, 1980
BstDEI	CTNAG	6 cut(s)	42, 345, 781, 810, 1694, 1957
BstDSI	CCRYGG	3 cut(s)	761, 1196, 1281
BstENI	CCTNNNNNAGG	1 cut(s)	1769
BstF5I	GGATG	5 cut(s)	547, 703, 955, 1015, 1802
BstFNI	CGCG	3 cut(s)	56, 114, 894
BstHHI	GCGC	1 cut(s)	116
BstKTI	GATC	8 cut(s)	86, 310, 640, 805, 838, 976, 1054, 1129
BstMAI	GTCTC	3 cut(s)	416, 487, 1742
BstMBI	GATC	8 cut(s)	83, 307, 637, 802, 835, 973, 1051, 1126
BstNI	CCWGG	2 cut(s)	312, 1992
BstNSI	RCATGY	2 cut(s)	1136, 1211
BstSCI	CCNGG	2 cut(s)	310, 1990
BstSFI	CTRYAG	3 cut(s)	171, 1023, 1515
BstUI	CGCG	3 cut(s)	56, 114, 894
BstV1I	GCAGC	7 cut(s)	246, 587, 1045, 1048, 1903, 1943, 2007
BstX2I	RGATCY	3 cut(s)	83, 307, 802
BstYI	RGATCY	3 cut(s)	83, 307, 802
BsuI	GTATCC	1 cut(s)	1409
BsuRI	GGCC	6 cut(s)	62, 260, 391, 543, 686, 1425
BtgI	CCRYGG	3 cut(s)	761, 1196, 1281
BtsCI	GGATG	5 cut(s)	547, 703, 955, 1015, 1802
BtsI	GCAGTG	1 cut(s)	1524
BtsIMutI	CAGTG	5 cut(s)	264, 438, 1513, 1524, 1758
BveI	ACCTGC	1 cut(s)	1202
Cac8I	GCNNGC	7 cut(s)	22, 73, 165, 249, 1209, 1976, 1980
CaiI	CAGNNNCTG	2 cut(s)	539, 788
CfoI	GCGC	1 cut(s)	116
Cfr10I	RCCGGY	1 cut(s)	71
Cfr13I	GGNCC	4 cut(s)	176, 389, 1075, 1285
CpoI	CGGWCCG	1 cut(s)	1075
CseI	GACGC	3 cut(s)	266, 1020, 1613
Csp6I	GTAC	2 cut(s)	362, 1787
CspCI	CAANNNNNGTGG	2 cut(s)	1246, 1281
CspI	CGGWCCG	1 cut(s)	1075
CviQI	GTAC	2 cut(s)	362, 1787
DdeI	CTNAG	6 cut(s)	42, 345, 781, 810, 1694, 1957
DpnI	GATC	8 cut(s)	85, 309, 639, 804, 837, 975, 1053, 1128
DpnII	GATC	8 cut(s)	83, 307, 637, 802, 835, 973, 1051, 1126
EaeI	YGGCCR	2 cut(s)	541, 1423
Eam1104I	CTCTTC	2 cut(s)	215, 950
EarI	CTCTTC	2 cut(s)	215, 950
Ecl136II	GAGCTC	1 cut(s)	24
Eco130I	CCWWGG	2 cut(s)	761, 1043
Eco24I	GRGCYC	3 cut(s)	26, 33, 1958
Eco47I	GGWCC	3 cut(s)	176, 1075, 1285
Eco53kI	GAGCTC	1 cut(s)	24
Eco57I	CTGAAG	1 cut(s)	288
Eco88I	CYCGRG	1 cut(s)	25
EcoICRI	GAGCTC	1 cut(s)	24
EcoNI	CCTNNNNNAGG	1 cut(s)	1769
EcoRII	CCWGG	2 cut(s)	310, 1990
EcoT14I	CCWWGG	2 cut(s)	761, 1043
EcoT38I	GRGCYC	3 cut(s)	26, 33, 1958
ErhI	CCWWGG	2 cut(s)	761, 1043
FalI	AAGNNNNNCTT	4 cut(s)	1383, 1415, 1457, 1489
FaqI	GGGAC	2 cut(s)	1018, 1806
FblI	GTMKAC	2 cut(s)	357, 1298
Fnu4HI	GCNGC	9 cut(s)	57, 60, 235, 576, 1059, 1062, 1917, 1932, 1996
FokI	GGATG	5 cut(s)	534, 710, 962, 1022, 1789
FriOI	GRGCYC	3 cut(s)	26, 33, 1958
Fsp4HI	GCNGC	9 cut(s)	57, 60, 235, 576, 1059, 1062, 1917, 1932, 1996
FspBI	CTAG	5 cut(s)	992, 1304, 1350, 1841, 1944
GlaI	GCGC	1 cut(s)	115
GluI	GCNGC	9 cut(s)	57, 60, 235, 576, 1059, 1062, 1917, 1932, 1996
GsaI	CCCAGC	1 cut(s)	1206
GsuI	CTGGAG	5 cut(s)	516, 705, 1703, 1850, 1886
HaeIII	GGCC	6 cut(s)	62, 260, 391, 543, 686, 1425
HapII	CCGG	4 cut(s)	72, 191, 1569, 1983
HgaI	GACGC	3 cut(s)	266, 1020, 1613
HhaI	GCGC	1 cut(s)	116
Hin1I	GRCGYC	2 cut(s)	887, 1031
Hin6I	GCGC	1 cut(s)	114
HinP1I	GCGC	1 cut(s)	114
HincII	GTYRAC	2 cut(s)	1444, 1470
HindII	GTYRAC	2 cut(s)	1444, 1470
HindIII	AAGCTT	1 cut(s)	245
HinfI	GANTC	5 cut(s)	131, 701, 812, 1685, 1750
HpaI	GTTAAC	1 cut(s)	1444
HpaII	CCGG	4 cut(s)	72, 191, 1569, 1983
HphI	GGTGA	6 cut(s)	705, 901, 1193, 1238, 1265, 1400
Hpy166II	GTNNAC	5 cut(s)	358, 944, 1299, 1444, 1470
Hpy188III	TCNNGA	6 cut(s)	324, 419, 986, 1623, 1815, 1959
Hpy8I	GTNNAC	5 cut(s)	358, 944, 1299, 1444, 1470
Hpy99I	CGWCG	2 cut(s)	83, 889
HpyAV	CCTTC	6 cut(s)	482, 820, 1047, 1325, 1699, 1879
HpyCH4III	ACNGT	6 cut(s)	172, 180, 730, 1282, 1327, 1508
HpyCH4IV	ACGT	3 cut(s)	360, 887, 1446
HpyF3I	CTNAG	6 cut(s)	42, 345, 781, 810, 1694, 1957
HpySE526I	ACGT	3 cut(s)	360, 887, 1446
Hsp92I	GRCGYC	2 cut(s)	887, 1031
HspAI	GCGC	1 cut(s)	114
KroI	GCCGGC	1 cut(s)	71
KroNI	GCCGGC	1 cut(s)	73
KspAI	GTTAAC	1 cut(s)	1444
Kzo9I	GATC	8 cut(s)	83, 307, 637, 802, 835, 973, 1051, 1126
LguI	GCTCTTC	1 cut(s)	215
LmnI	GCTCC	9 cut(s)	36, 98, 193, 231, 450, 532, 572, 1774, 1866
Lsp1109I	GCAGC	7 cut(s)	246, 587, 1045, 1048, 1903, 1943, 2007
LweI	GCATC	9 cut(s)	338, 940, 1009, 1516, 1654, 1808, 1811, 1928, 2002
MaeI	CTAG	5 cut(s)	992, 1304, 1350, 1841, 1944
MaeII	ACGT	3 cut(s)	360, 887, 1446
MalI	GATC	8 cut(s)	85, 309, 639, 804, 837, 975, 1053, 1128
MbiI	CCGCTC	2 cut(s)	46, 137
MboI	GATC	8 cut(s)	83, 307, 637, 802, 835, 973, 1051, 1126
MboII	GAAGA	8 cut(s)	232, 253, 314, 317, 840, 967, 1403, 1688
MflI	RGATCY	3 cut(s)	83, 307, 802
MhlI	GDGCHC	3 cut(s)	26, 33, 1958
MlsI	TGGCCA	1 cut(s)	543
MluCI	AATT	5 cut(s)	376, 1014, 1584, 1633, 1679
MluNI	TGGCCA	1 cut(s)	543
MlyI	GAGTC	3 cut(s)	140, 821, 1744
MmeI	TCCRAC	2 cut(s)	644, 1029
Mox20I	TGGCCA	1 cut(s)	543
MroNI	GCCGGC	1 cut(s)	71
MscI	TGGCCA	1 cut(s)	543
MseI	TTAA	6 cut(s)	863, 968, 1361, 1443, 1638, 1724
MslI	CAYNNNNRTG	1 cut(s)	1212
Msp20I	TGGCCA	1 cut(s)	543
MspA1I	CMGCKG	1 cut(s)	237
MspI	CCGG	4 cut(s)	72, 191, 1569, 1983
MspR9I	CCNGG	2 cut(s)	312, 1992
Mva1269I	GAATGC	2 cut(s)	214, 1945
MvaI	CCWGG	2 cut(s)	312, 1992
MvnI	CGCG	3 cut(s)	56, 114, 894
NaeI	GCCGGC	1 cut(s)	73
NcoI	CCATGG	1 cut(s)	761
NdeII	GATC	8 cut(s)	83, 307, 637, 802, 835, 973, 1051, 1126
NgoMIV	GCCGGC	1 cut(s)	71
NlaIV	GGNNCC	5 cut(s)	32, 195, 804, 1105, 1500
NmeAIII	GCCGAG	1 cut(s)	222
NmuCI	GTSAC	3 cut(s)	888, 907, 1253
NspI	RCATGY	2 cut(s)	1136, 1211
PaeI	GCATGC	1 cut(s)	1211
PaqCI	CACCTGC	1 cut(s)	1202
PciSI	GCTCTTC	1 cut(s)	215
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	2 cut(s)	214, 1945
PdiI	GCCGGC	1 cut(s)	73
PfeI	GAWTC	2 cut(s)	701, 1685
PflFI	GACNNNGTC	1 cut(s)	670
PflMI	CCANNNNNTGG	4 cut(s)	539, 743, 1202, 1870
PkrI	GCNGC	9 cut(s)	58, 61, 236, 577, 1060, 1063, 1918, 1933, 1997
PleI	GAGTC	3 cut(s)	139, 820, 1744
PpsI	GAGTC	3 cut(s)	139, 820, 1744
Psp124BI	GAGCTC	1 cut(s)	26
Psp1406I	AACGTT	1 cut(s)	1446
Psp6I	CCWGG	2 cut(s)	310, 1990
PspFI	CCCAGC	1 cut(s)	1202
PspGI	CCWGG	2 cut(s)	310, 1990
PspN4I	GGNNCC	5 cut(s)	32, 195, 804, 1105, 1500
PspPI	GGNCC	4 cut(s)	176, 389, 1075, 1285
PstI	CTGCAG	1 cut(s)	1519
PstNI	CAGNNNCTG	2 cut(s)	539, 788
PsuI	RGATCY	3 cut(s)	83, 307, 802
PsyI	GACNNNGTC	1 cut(s)	670
PvuII	CAGCTG	1 cut(s)	237
RsaI	GTAC	2 cut(s)	363, 1788
RsaNI	GTAC	2 cut(s)	362, 1787
RseI	CAYNNNNRTG	1 cut(s)	1212
Rsr2I	CGGWCCG	1 cut(s)	1075
RsrII	CGGWCCG	1 cut(s)	1075
SacI	GAGCTC	1 cut(s)	26
SapI	GCTCTTC	1 cut(s)	215
SaqAI	TTAA	6 cut(s)	863, 968, 1361, 1443, 1638, 1724
SatI	GCNGC	9 cut(s)	57, 60, 235, 576, 1059, 1062, 1917, 1932, 1996
Sau3AI	GATC	8 cut(s)	83, 307, 637, 802, 835, 973, 1051, 1126
Sau96I	GGNCC	4 cut(s)	176, 389, 1075, 1285
SchI	GAGTC	3 cut(s)	140, 821, 1744
ScrFI	CCNGG	2 cut(s)	312, 1992
SduI	GDGCHC	3 cut(s)	26, 33, 1958
SfaNI	GCATC	9 cut(s)	338, 940, 1009, 1516, 1654, 1808, 1811, 1928, 2002
SfcI	CTRYAG	3 cut(s)	171, 1023, 1515
SinI	GGWCC	3 cut(s)	176, 1075, 1285
SmiMI	CAYNNNNRTG	1 cut(s)	1212
SmlI	CTYRAG	1 cut(s)	1463
SmoI	CTYRAG	1 cut(s)	1463
SpeI	ACTAGT	1 cut(s)	1303
SphI	GCATGC	1 cut(s)	1211
Sse9I	AATT	5 cut(s)	376, 1014, 1584, 1633, 1679
SsiI	CCGC	9 cut(s)	46, 56, 59, 135, 251, 894, 1073, 1078, 1844
SspMI	CTAG	5 cut(s)	992, 1304, 1350, 1841, 1944
SstI	GAGCTC	1 cut(s)	26
StyD4I	CCNGG	2 cut(s)	310, 1990
StyI	CCWWGG	2 cut(s)	761, 1043
TaaI	ACNGT	6 cut(s)	172, 180, 730, 1282, 1327, 1508
TaiI	ACGT	3 cut(s)	363, 890, 1449
TaqI	TCGA	3 cut(s)	78, 651, 1836
TasI	AATT	5 cut(s)	376, 1014, 1584, 1633, 1679
TauI	GCSGC	2 cut(s)	59, 62
TfiI	GAWTC	2 cut(s)	701, 1685
Tru1I	TTAA	6 cut(s)	863, 968, 1361, 1443, 1638, 1724
Tru9I	TTAA	6 cut(s)	863, 968, 1361, 1443, 1638, 1724
TscAI	CASTG	5 cut(s)	271, 445, 1513, 1524, 1765
TseFI	GTSAC	3 cut(s)	888, 907, 1253
TseI	GCWGC	7 cut(s)	234, 575, 1058, 1061, 1916, 1931, 1995
Tsp45I	GTSAC	3 cut(s)	888, 907, 1253
TspDTI	ATGAA	7 cut(s)	383, 714, 1026, 1058, 1228, 1578, 1631
TspGWI	ACGGA	2 cut(s)	96, 895
TspRI	CASTG	5 cut(s)	271, 445, 1513, 1524, 1765
Tth111I	GACNNNGTC	1 cut(s)	670
Van91I	CCANNNNNTGG	4 cut(s)	539, 743, 1202, 1870
VpaK11BI	GGWCC	3 cut(s)	176, 1075, 1285
XagI	CCTNNNNNAGG	1 cut(s)	1769
XapI	RAATTY	2 cut(s)	376, 1633
XceI	RCATGY	2 cut(s)	1136, 1211
XmiI	GTMKAC	2 cut(s)	357, 1298
XspI	CTAG	5 cut(s)	992, 1304, 1350, 1841, 1944
ZraI	GACGTC	1 cut(s)	888

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.