Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007134

Physical Location & Seq

Genomic Coordinates Reverse (-)

29411743 .. 29417583

Gene Structure 5841 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1216500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2019 bp

ATGGCTATGGCTCGGGCGAGCTCGGGGCTCCAGTACCCCGAGCGGTTTTACGCGGCGGCCTCCTACGCCGGCTTCGACGGATCTCCTCGCTCCACCTCCAAAGCCGTTCGCGCCAAGTTCAGCTCTGAGTCCGCACTCATACTCTACGCCTTGTACCAGCAGGCTACCGTAGGGCCTTGTAACATACCGGAACCTAGCGCTTGGAATGCTGTTGAGAAAAGCAAATGGAGCAGCTGGAAGAAGATTTCGGACATGGCTTCCACTGAAGCAATGCGTCTTTTTGTGAAAATATTAGAGGAGGAAGATCCAGGTTGGTATTCAAGAGCATCAAACTTTGTTTCAGAGGCTGTGGTAGATGTACAAATGAACCAATATTCAAGTATTCAGCCAGTTGTTGAGAATGGTAACTCTTTTCCCGAGACCAAGACAATCTCCACTGAAAACGGGAGCGTAGTGGAAGCTCAGGATAAAGATGTTGTCTCCGAAGGCTTTGGATCAGTTGTTGTCTATGATCAATGGATTGAACCTCCAGTGTCTGGCCATCGTCCAAAATCCCGATATGAGCATGGAGCGGCGGTTATTCAAGACAAGATGTACATCTATGGTGGAAACCACAATGGTCGTTACCTAAATGATCTCCATGTCTTGGACTTGAGGAGTTGGAGTTGGTCAAAGATTGACGCCAAATCTGTGGCTGAGTCAGCTGAGGCACCCTCACCAGTAACAGTAACCCCATGCGCGGGTCATTCGTTGATACCTTGGGATAATAAACTTATTTCAATTGCTGGACATACAAAGAATCCTACTGATTCTATTCAGGTGAAGGCATTCGATCTACAAACTTGCTCATGGTCATTCTTAAAGACTTATGGGAAACCACCGGCATCACGTGGAGGTCAATCCGTGTCTCTTGTTGGAGGCAGCTTTGTGATTTTCGGGGGACAAGATGCAAAGAGATCTCTCTTGAATGACCTGCATATTCTTGACCTAGAAACAATGACGTGGGATGAGATTGATGCTGTAGGGGTGCCTCCTTCTCCGAGGTCTGATCATACTGCTGCAGTCCATGCTGACCGCTATCTTCTTATATTTGGTGGTGGTTCTCATGCCACTTGTTACAATGATCTCCATGTCCTTGATTTGCAAAGTATGGAGTGGTCAAGACCCACACAACAAGGTGAAATACCAACTCCACGGGCTGGACATGCAGGTGTAACTGTTGGAGAGAATTGGTTCATAGTTGGTGGTGGGGACAACAAGAGTGGTGCTTCAGAAACTGTTGTCCTAAATATGTCCACACTTGTTTGGTCTGTTGTAACTACCGTTGAAGGGCGCATTCCTGTTGCTAGTGAGGGACTAAGTTTGGCCATAGGTTCATATAATGGTGAAGACGTTCTTGTATCATTTGGAGGATACAATGGGTGTTACAACAATGAGGTTAACGTTCTTAAACCAAGCCACAAGTCAACCTTGCAATCGAAGATGATGGAAACTCCTGTTCCGGACAGTGTTTCTGCTGTGCTTAATCAAAGTGCTACCAGAGATGTAGAGTCTGAAACAGGGCAAGAAGGAAAAATTAGGGAAATTGTTATGGACAACGTTGATGCGGATCCCATGAAATCAAGAGGCAAGGTTACCAATGAACATCTTACAGCAACCCTCAAAGCAGAGAAAGAAGAATTAGAGTCATCACTCAGTAAGGAGAAAGTGCAAACGCTCCAATTAAAGGAAGATTTAGCAGAATTAGAGACTCGTAACACTGACCTGTACAAGGAGCTTCAATCAGTACGTGGTCAACTTGGATCTGAGCAGTCAAGATGTTTCAAACTCGAGGTTGAACTTGCAGAACTACGACAGAAGCTCCAAACAATGGAGACATTGCAGAAAGAACTCGAACTCCTCCAGCGACAAAAGGCTGCATCAGAACAAGCAGCGTTAAATGCTAAAAGGCAGGGCTCAGGTGGCGTCTGGGGCTGGCTCGCGGGAACCCCTGCTAATGAAAAAGCTGATGACGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000062 GO:0000166 GO:0001101 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006810 GO:0006869 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009753 GO:0010033 GO:0010876 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033218 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048037 GO:0050662 GO:0050896 GO:0051179 GO:0051234 GO:0071702 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901567 GO:1901681 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ACBP Kelch_3 Kelch_4 Kelch_5 Kelch_6

Protein Analysis

673

Amino Acids

73.37

Weight (kDa)

5.2

Isoelectric Point (pI)

45.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACBP	PF00887	16 - 97	2.5e-18	Acyl CoA binding protein
Beta-prop_FBX42	PF13415	166 - 230	2.8e-12	FBX42, beta-propeller domain
Kelch_HCF	PF13854	168 - 366	1.3e-20	Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1	PF24981	171 - 302	4e-18	Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7	PF24681	172 - 235	2.1e-16	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1	PF01344	186 - 226	7.6e-09	Kelch motif
Beta-prop_FBX42	PF13415	242 - 305	1.9e-07	FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	248 - 471	1e-55	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1	PF24981	261 - 369	1.1e-13	Attractin/LZTR1 beta-propeller
Beta-prop_FBX42	PF13415	298 - 369	5.9e-16	FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1	PF24981	311 - 483	2.5e-20	Attractin/LZTR1 beta-propeller
Beta-prop_FBX42	PF13415	351 - 496	6.8e-21	FBX42, beta-propeller domain
Kelch_HCF	PF13854	360 - 417	1.3e-07	Host cell factor, Kelch-repeats domain
ACBP4_C	PF24922	548 - 651	6.2e-40	Acyl-CoA-binding domain-containing protein 4-6, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007134)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05420 AT3G05420 AT5G27630 AT5G27630 AT5G27630 AT5G27630
fragaria_vesca	FvH4_6g06301 FvH4_6g06301 FvH4_6g06301
malus_domestica	MD04G1202500.v1.1 MD12G1216500.v1.1
prunus_persica	Prupe.6G322800_v2.0.a1 Prupe.6G322800_v2.0.a1
pyrus_communis	pycom04g17870 pycom12g20200
rosa_chinensis	RchiOBHm_Chr3g0455341
rosa_laevigata	RLG00000025368
rosa_multiflora	Rmu_sc0002169.1_g000045 Rmu_sc0024960.1_g000003
rosa_roxburghii	Rroxscaffold_6G00424350
rosa_rugosa	Rorug03G0006600 Rorug03G0006700
rosa_samantha	Rh3CG066800 Rh3DG068300
rosa_wichuraiana	Rw3G005220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1199
Acc36I	ACCTGC	2 cut(s)	981, 1199
AccB1I	GGYRCC	2 cut(s)	709, 1027
AccB7I	CCANNNNNTGG	4 cut(s)	536, 646, 1199, 1870
AccBSI	CCGCTC	2 cut(s)	43, 572
AccII	CGCG	4 cut(s)	53, 111, 740, 1982
AccIII	TCCGGA	1 cut(s)	1501
AclI	AACGTT	2 cut(s)	1443, 1599
AclWI	GGATC	6 cut(s)	88, 299, 502, 1604, 1617, 1810
AcoI	YGGCCR	2 cut(s)	538, 1365
AcuI	CTGAAG	2 cut(s)	285, 1254
AcvI	CACGTG	1 cut(s)	890
AcyI	GRCGYC	3 cut(s)	681, 1965, 2013
AfaI	GTAC	6 cut(s)	35, 155, 360, 596, 1769, 1788
AfeI	AGCGCT	1 cut(s)	199
AfiI	CCNNNNNNNGG	9 cut(s)	536, 646, 739, 740, 1199, 1329, 1726, 1771, 1870
AjiI	CACGTC	1 cut(s)	1002
AjnI	CCWGG	1 cut(s)	307
AloI	GAACNNNNNNTCC	2 cut(s)	1881, 1913
AluBI	AGCT	9 cut(s)	21, 123, 234, 461, 704, 924, 1777, 1861, 2006
AluI	AGCT	9 cut(s)	21, 123, 234, 461, 704, 924, 1777, 1861, 2006
Alw21I	GWGCWC	1 cut(s)	23
Alw26I	GTCTC	5 cut(s)	413, 484, 912, 1742, 1868
AlwI	GGATC	6 cut(s)	88, 299, 502, 1604, 1617, 1810
AlwNI	CAGNNNCTG	3 cut(s)	347, 536, 1277
Ama87I	CYCGRG	5 cut(s)	12, 22, 38, 416, 1829
Aor13HI	TCCGGA	1 cut(s)	1501
Aor51HI	AGCGCT	1 cut(s)	199
AoxI	GGCC	4 cut(s)	57, 173, 538, 1365
ApeKI	GCWGC	5 cut(s)	231, 921, 1058, 1916, 1931
Asp700I	GAANNNNTTC	3 cut(s)	827, 1232, 1392
AspLEI	GCGC	4 cut(s)	113, 200, 740, 1335
AspS9I	GGNCC	1 cut(s)	173
AsuHPI	GGTGA	4 cut(s)	708, 832, 1190, 1397
AvaI	CYCGRG	5 cut(s)	12, 22, 38, 416, 1829
BalI	TGGCCA	2 cut(s)	540, 1367
BamHI	GGATCC	1 cut(s)	1609
BanI	GGYRCC	2 cut(s)	709, 1027
BanII	GRGCYC	3 cut(s)	23, 30, 1958
BbrPI	CACGTG	1 cut(s)	890
BbsI	GAAGAC	1 cut(s)	1395
Bbv12I	GWGCWC	1 cut(s)	23
BbvCI	CCTCAGC	1 cut(s)	705
BbvI	GCAGC	5 cut(s)	243, 933, 1045, 1903, 1943
BccI	CCATC	2 cut(s)	549, 1480
BceAI	ACGGC	1 cut(s)	89
BciT130I	CCWGG	1 cut(s)	309
BciVI	GTATCC	1 cut(s)	1406
BclI	TGATCA	2 cut(s)	511, 1048
BcoDI	GTCTC	5 cut(s)	413, 484, 912, 1742, 1868
BfaI	CTAG	3 cut(s)	195, 989, 1347
BfmI	CTRYAG	2 cut(s)	1020, 1059
BfoI	RGCGCY	1 cut(s)	201
BfuAI	ACCTGC	2 cut(s)	981, 1199
BfuI	GTATCC	1 cut(s)	1406
BglII	AGATCT	1 cut(s)	956
BisI	GCNGC	8 cut(s)	54, 57, 232, 573, 922, 1059, 1917, 1932
BlsI	GCNGC	8 cut(s)	55, 58, 233, 574, 923, 1060, 1918, 1933
Bme1390I	CCNGG	1 cut(s)	309
BmeT110I	CYCGRG	5 cut(s)	12, 22, 38, 416, 1829
BmgBI	CACGTC	1 cut(s)	1002
BmgT120I	GGNCC	1 cut(s)	173
BmiI	GGNNCC	6 cut(s)	29, 192, 711, 1029, 1611, 1987
BmrFI	CCNGG	1 cut(s)	309
BmsI	GCATC	6 cut(s)	335, 893, 937, 1006, 1594, 1928
BpiI	GAAGAC	1 cut(s)	1395
BplI	GAGNNNNNCTC	2 cut(s)	698, 730
BpmI	CTGGAG	3 cut(s)	14, 513, 1886
Bpu10I	CCTNAGC	3 cut(s)	462, 705, 1957
BpuEI	CTTGAG	1 cut(s)	673
BsaAI	YACGTR	2 cut(s)	890, 1790
BsaBI	GATNNNNATC	2 cut(s)	596, 1608
BsaHI	GRCGYC	3 cut(s)	681, 1965, 2013
BsaI	GGTCTC	1 cut(s)	413
BsaJI	CCNNGG	3 cut(s)	758, 1040, 1193
BsaWI	WCCGGW	2 cut(s)	187, 1501
BsaXI	ACNNNNNCTCC	8 cut(s)	561, 591, 649, 679, 1845, 1875, 1881, 1911
Bsc4I	CCNNNNNNNGG	9 cut(s)	536, 646, 739, 740, 1199, 1329, 1726, 1771, 1870
Bse118I	RCCGGY	2 cut(s)	68, 880
Bse1I	ACTGG	4 cut(s)	31, 389, 530, 719
Bse3DI	GCAATG	2 cut(s)	276, 1877
Bse8I	GATNNNNATC	2 cut(s)	596, 1608
BseAI	TCCGGA	1 cut(s)	1501
BseBI	CCWGG	1 cut(s)	309
BseDI	CCNNGG	3 cut(s)	758, 1040, 1193
BseGI	GGATG	1 cut(s)	1012
BseJI	GATNNNNATC	2 cut(s)	596, 1608
BseLI	CCNNNNNNNGG	9 cut(s)	536, 646, 739, 740, 1199, 1329, 1726, 1771, 1870
BseMI	GCAATG	2 cut(s)	276, 1877
BseMII	CTCAG	7 cut(s)	117, 476, 687, 696, 1708, 1797, 1971
BseNI	ACTGG	4 cut(s)	31, 389, 530, 719
BseRI	GAGGAG	4 cut(s)	75, 311, 670, 1889
BseXI	GCAGC	5 cut(s)	243, 933, 1045, 1903, 1943
Bsh1236I	CGCG	4 cut(s)	53, 111, 740, 1982
BshFI	GGCC	4 cut(s)	59, 175, 540, 1367
BshNI	GGYRCC	2 cut(s)	709, 1027
BsiHKAI	GWGCWC	1 cut(s)	23
BsiHKCI	CYCGRG	5 cut(s)	12, 22, 38, 416, 1829
BsiSI	CCGG	4 cut(s)	69, 188, 881, 1502
BslFI	GGGAC	3 cut(s)	954, 1265, 1368
BslI	CCNNNNNNNGG	9 cut(s)	536, 646, 739, 740, 1199, 1329, 1726, 1771, 1870
BsmAI	GTCTC	5 cut(s)	413, 484, 912, 1742, 1868
BsmFI	GGGAC	3 cut(s)	954, 1265, 1368
BsmI	GAATGC	3 cut(s)	211, 827, 1335
BsnI	GGCC	4 cut(s)	59, 175, 540, 1367
Bso31I	GGTCTC	1 cut(s)	413
BsoBI	CYCGRG	5 cut(s)	12, 22, 38, 416, 1829
Bsp1286I	GDGCHC	3 cut(s)	23, 30, 1958
Bsp13I	TCCGGA	1 cut(s)	1501
Bsp1407I	TGTACA	3 cut(s)	358, 594, 1767
BspANI	GGCC	4 cut(s)	59, 175, 540, 1367
BspCNI	CTCAG	7 cut(s)	118, 475, 688, 697, 1707, 1798, 1970
BspEI	TCCGGA	1 cut(s)	1501
BspFNI	CGCG	4 cut(s)	53, 111, 740, 1982
BspLI	GGNNCC	6 cut(s)	29, 192, 711, 1029, 1611, 1987
BspMAI	CTGCAG	1 cut(s)	1063
BspMI	ACCTGC	2 cut(s)	981, 1199
BspPI	GGATC	6 cut(s)	88, 299, 502, 1604, 1617, 1810
BspT107I	GGYRCC	2 cut(s)	709, 1027
BspTNI	GGTCTC	1 cut(s)	413
BsrBI	CCGCTC	2 cut(s)	43, 572
BsrDI	GCAATG	2 cut(s)	276, 1877
BsrFI	RCCGGY	2 cut(s)	68, 880
BsrGI	TGTACA	3 cut(s)	358, 594, 1767
BsrI	ACTGG	4 cut(s)	31, 389, 530, 719
BssAI	RCCGGY	2 cut(s)	68, 880
BssECI	CCNNGG	3 cut(s)	758, 1040, 1193
BssNI	GRCGYC	3 cut(s)	681, 1965, 2013
BssT1I	CCWWGG	1 cut(s)	758
Bst2UI	CCWGG	1 cut(s)	309
Bst4CI	ACNGT	6 cut(s)	169, 727, 1219, 1279, 1324, 1508
BstACI	GRCGYC	3 cut(s)	681, 1965, 2013
BstAPI	GCANNNNNTGC	1 cut(s)	1067
BstAUI	TGTACA	3 cut(s)	358, 594, 1767
BstBAI	YACGTR	2 cut(s)	890, 1790
BstC8I	GCNNGC	5 cut(s)	19, 70, 162, 1976, 1980
BstDEI	CTNAG	8 cut(s)	126, 462, 696, 705, 1358, 1694, 1806, 1957
BstDSI	CCRYGG	1 cut(s)	1193
BstEII	GGTNACC	1 cut(s)	1633
BstENI	CCTNNNNNAGG	1 cut(s)	1769
BstF5I	GGATG	1 cut(s)	1012
BstFNI	CGCG	4 cut(s)	53, 111, 740, 1982
BstH2I	RGCGCY	1 cut(s)	201
BstHHI	GCGC	4 cut(s)	113, 200, 740, 1335
BstMAI	GTCTC	5 cut(s)	413, 484, 912, 1742, 1868
BstNI	CCWGG	1 cut(s)	309
BstNSI	RCATGY	1 cut(s)	1208
BstPI	GGTNACC	1 cut(s)	1633
BstSCI	CCNGG	1 cut(s)	307
BstSFI	CTRYAG	2 cut(s)	1020, 1059
BstUI	CGCG	4 cut(s)	53, 111, 740, 1982
BstV1I	GCAGC	5 cut(s)	243, 933, 1045, 1903, 1943
BstV2I	GAAGAC	1 cut(s)	1395
BstX2I	RGATCY	5 cut(s)	80, 304, 956, 1609, 1802
BstXI	CCANNNNNNTGG	1 cut(s)	691
BstYI	RGATCY	5 cut(s)	80, 304, 956, 1609, 1802
BsuI	GTATCC	1 cut(s)	1406
BsuRI	GGCC	4 cut(s)	59, 175, 540, 1367
BtgI	CCRYGG	1 cut(s)	1193
BtrI	CACGTC	1 cut(s)	1002
BtsCI	GGATG	1 cut(s)	1012
BtsIMutI	CAGTG	5 cut(s)	261, 435, 537, 1513, 1758
BveI	ACCTGC	2 cut(s)	981, 1199
Cac8I	GCNNGC	5 cut(s)	19, 70, 162, 1976, 1980
CaiI	CAGNNNCTG	3 cut(s)	347, 536, 1277
CfoI	GCGC	4 cut(s)	113, 200, 740, 1335
Cfr10I	RCCGGY	2 cut(s)	68, 880
Cfr13I	GGNCC	1 cut(s)	173
CseI	GACGC	3 cut(s)	263, 689, 1954
Csp6I	GTAC	6 cut(s)	34, 154, 359, 595, 1768, 1787
CspCI	CAANNNNNGTGG	2 cut(s)	1243, 1278
CviQI	GTAC	6 cut(s)	34, 154, 359, 595, 1768, 1787
DdeI	CTNAG	8 cut(s)	126, 462, 696, 705, 1358, 1694, 1806, 1957
EaeI	YGGCCR	2 cut(s)	538, 1365
Ecl136II	GAGCTC	1 cut(s)	21
Eco130I	CCWWGG	1 cut(s)	758
Eco24I	GRGCYC	3 cut(s)	23, 30, 1958
Eco31I	GGTCTC	1 cut(s)	413
Eco47III	AGCGCT	1 cut(s)	199
Eco53kI	GAGCTC	1 cut(s)	21
Eco57I	CTGAAG	2 cut(s)	285, 1254
Eco72I	CACGTG	1 cut(s)	890
Eco88I	CYCGRG	5 cut(s)	12, 22, 38, 416, 1829
Eco91I	GGTNACC	1 cut(s)	1633
EcoICRI	GAGCTC	1 cut(s)	21
EcoNI	CCTNNNNNAGG	1 cut(s)	1769
EcoO109I	RGGNCCY	1 cut(s)	173
EcoO65I	GGTNACC	1 cut(s)	1633
EcoRII	CCWGG	1 cut(s)	307
EcoT14I	CCWWGG	1 cut(s)	758
EcoT38I	GRGCYC	3 cut(s)	23, 30, 1958
ErhI	CCWWGG	1 cut(s)	758
FalI	AAGNNNNNCTT	4 cut(s)	1380, 1412, 1454, 1486
FaqI	GGGAC	3 cut(s)	954, 1265, 1368
FauI	CCCGC	2 cut(s)	733, 1975
FbaI	TGATCA	2 cut(s)	511, 1048
Fnu4HI	GCNGC	8 cut(s)	54, 57, 232, 573, 922, 1059, 1917, 1932
FokI	GGATG	1 cut(s)	1019
FriOI	GRGCYC	3 cut(s)	23, 30, 1958
Fsp4HI	GCNGC	8 cut(s)	54, 57, 232, 573, 922, 1059, 1917, 1932
FspBI	CTAG	3 cut(s)	195, 989, 1347
GlaI	GCGC	4 cut(s)	112, 199, 739, 1334
GluI	GCNGC	8 cut(s)	54, 57, 232, 573, 922, 1059, 1917, 1932
GsuI	CTGGAG	3 cut(s)	14, 513, 1886
HaeII	RGCGCY	1 cut(s)	201
HaeIII	GGCC	4 cut(s)	59, 175, 540, 1367
HapII	CCGG	4 cut(s)	69, 188, 881, 1502
HgaI	GACGC	3 cut(s)	263, 689, 1954
HhaI	GCGC	4 cut(s)	113, 200, 740, 1335
Hin1I	GRCGYC	3 cut(s)	681, 1965, 2013
Hin6I	GCGC	4 cut(s)	111, 198, 738, 1333
HinP1I	GCGC	4 cut(s)	111, 198, 738, 1333
HincII	GTYRAC	3 cut(s)	1441, 1467, 1796
HindII	GTYRAC	3 cut(s)	1441, 1467, 1796
HinfI	GANTC	7 cut(s)	128, 698, 799, 809, 1550, 1685, 1750
HpaI	GTTAAC	1 cut(s)	1441
HpaII	CCGG	4 cut(s)	69, 188, 881, 1502
HphI	GGTGA	4 cut(s)	708, 832, 1190, 1397
Hpy166II	GTNNAC	4 cut(s)	1296, 1441, 1467, 1796
Hpy8I	GTNNAC	4 cut(s)	1296, 1441, 1467, 1796
Hpy99I	CGWCG	1 cut(s)	80
HpyAV	CCTTC	5 cut(s)	479, 817, 1044, 1322, 1562
HpyCH4III	ACNGT	6 cut(s)	169, 727, 1219, 1279, 1324, 1508
HpyCH4IV	ACGT	6 cut(s)	889, 1001, 1392, 1443, 1599, 1789
HpyF3I	CTNAG	8 cut(s)	126, 462, 696, 705, 1358, 1694, 1806, 1957
HpySE526I	ACGT	6 cut(s)	889, 1001, 1392, 1443, 1599, 1789
Hsp92I	GRCGYC	3 cut(s)	681, 1965, 2013
HspAI	GCGC	4 cut(s)	111, 198, 738, 1333
Kpn2I	TCCGGA	1 cut(s)	1501
KroI	GCCGGC	1 cut(s)	68
KroNI	GCCGGC	1 cut(s)	70
Ksp22I	TGATCA	2 cut(s)	511, 1048
KspAI	GTTAAC	1 cut(s)	1441
LmnI	GCTCC	8 cut(s)	33, 95, 228, 447, 569, 1722, 1774, 1866
Lsp1109I	GCAGC	5 cut(s)	243, 933, 1045, 1903, 1943
LweI	GCATC	6 cut(s)	335, 893, 937, 1006, 1594, 1928
MaeI	CTAG	3 cut(s)	195, 989, 1347
MaeII	ACGT	6 cut(s)	889, 1001, 1392, 1443, 1599, 1789
MbiI	CCGCTC	2 cut(s)	43, 572
MboII	GAAGA	8 cut(s)	250, 253, 314, 1073, 1400, 1492, 1688, 1742
MfeI	CAATTG	1 cut(s)	780
MflI	RGATCY	5 cut(s)	80, 304, 956, 1609, 1802
MhlI	GDGCHC	3 cut(s)	23, 30, 1958
MlsI	TGGCCA	2 cut(s)	540, 1367
MluCI	AATT	7 cut(s)	780, 1228, 1575, 1584, 1679, 1721, 1742
MluNI	TGGCCA	2 cut(s)	540, 1367
MlyI	GAGTC	5 cut(s)	137, 707, 1559, 1694, 1744
MmeI	TCCRAC	3 cut(s)	641, 895, 1201
Mox20I	TGGCCA	2 cut(s)	540, 1367
MroI	TCCGGA	1 cut(s)	1501
MroNI	GCCGGC	1 cut(s)	68
MroXI	GAANNNNTTC	3 cut(s)	827, 1232, 1392
MscI	TGGCCA	2 cut(s)	540, 1367
MseI	TTAA	6 cut(s)	860, 1440, 1449, 1524, 1724, 1937
MslI	CAYNNNNRTG	1 cut(s)	1209
Msp20I	TGGCCA	2 cut(s)	540, 1367
MspA1I	CMGCKG	2 cut(s)	234, 704
MspI	CCGG	4 cut(s)	69, 188, 881, 1502
MspR9I	CCNGG	1 cut(s)	309
MunI	CAATTG	1 cut(s)	780
Mva1269I	GAATGC	3 cut(s)	211, 827, 1335
MvaI	CCWGG	1 cut(s)	309
MvnI	CGCG	4 cut(s)	53, 111, 740, 1982
NaeI	GCCGGC	1 cut(s)	70
NgoMIV	GCCGGC	1 cut(s)	68
NlaIV	GGNNCC	6 cut(s)	29, 192, 711, 1029, 1611, 1987
NspI	RCATGY	1 cut(s)	1208
PaeR7I	CTCGAG	1 cut(s)	1829
PaqCI	CACCTGC	1 cut(s)	1199
PcsI	WCGNNNNNNNCGW	1 cut(s)	72
PctI	GAATGC	3 cut(s)	211, 827, 1335
PdiI	GCCGGC	1 cut(s)	70
PdmI	GAANNNNTTC	3 cut(s)	827, 1232, 1392
PfeI	GAWTC	2 cut(s)	799, 809
PflMI	CCANNNNNTGG	4 cut(s)	536, 646, 1199, 1870
PkrI	GCNGC	8 cut(s)	55, 58, 233, 574, 923, 1060, 1918, 1933
PleI	GAGTC	5 cut(s)	136, 706, 1558, 1693, 1744
PmaCI	CACGTG	1 cut(s)	890
PmlI	CACGTG	1 cut(s)	890
PpsI	GAGTC	5 cut(s)	136, 706, 1558, 1693, 1744
Ppu21I	YACGTR	2 cut(s)	890, 1790
Psp124BI	GAGCTC	1 cut(s)	23
Psp1406I	AACGTT	2 cut(s)	1443, 1599
Psp6I	CCWGG	1 cut(s)	307
PspCI	CACGTG	1 cut(s)	890
PspEI	GGTNACC	1 cut(s)	1633
PspGI	CCWGG	1 cut(s)	307
PspN4I	GGNNCC	6 cut(s)	29, 192, 711, 1029, 1611, 1987
PspPI	GGNCC	1 cut(s)	173
PspXI	VCTCGAGB	1 cut(s)	1829
PstI	CTGCAG	1 cut(s)	1063
PstNI	CAGNNNCTG	3 cut(s)	347, 536, 1277
PsuI	RGATCY	5 cut(s)	80, 304, 956, 1609, 1802
PvuII	CAGCTG	2 cut(s)	234, 704
RsaI	GTAC	6 cut(s)	35, 155, 360, 596, 1769, 1788
RsaNI	GTAC	6 cut(s)	34, 154, 359, 595, 1768, 1787
RseI	CAYNNNNRTG	1 cut(s)	1209
SacI	GAGCTC	1 cut(s)	23
SaqAI	TTAA	6 cut(s)	860, 1440, 1449, 1524, 1724, 1937
SatI	GCNGC	8 cut(s)	54, 57, 232, 573, 922, 1059, 1917, 1932
Sau96I	GGNCC	1 cut(s)	173
SchI	GAGTC	5 cut(s)	137, 707, 1559, 1694, 1744
ScrFI	CCNGG	1 cut(s)	309
SduI	GDGCHC	3 cut(s)	23, 30, 1958
SfaNI	GCATC	6 cut(s)	335, 893, 937, 1006, 1594, 1928
SfcI	CTRYAG	2 cut(s)	1020, 1059
Sfr274I	CTCGAG	1 cut(s)	1829
SlaI	CTCGAG	1 cut(s)	1829
SmiMI	CAYNNNNRTG	1 cut(s)	1209
SmlI	CTYRAG	2 cut(s)	652, 1829
SmoI	CTYRAG	2 cut(s)	652, 1829
Sse9I	AATT	7 cut(s)	780, 1228, 1575, 1584, 1679, 1721, 1742
SspI	AATATT	2 cut(s)	291, 374
SspMI	CTAG	3 cut(s)	195, 989, 1347
SstI	GAGCTC	1 cut(s)	23
StyD4I	CCNGG	1 cut(s)	307
StyI	CCWWGG	1 cut(s)	758
TaaI	ACNGT	6 cut(s)	169, 727, 1219, 1279, 1324, 1508
TaiI	ACGT	6 cut(s)	892, 1004, 1395, 1446, 1602, 1792
TaqI	TCGA	5 cut(s)	75, 831, 1478, 1830, 1893
TasI	AATT	7 cut(s)	780, 1228, 1575, 1584, 1679, 1721, 1742
TatI	WGTACW	3 cut(s)	358, 594, 1767
TauI	GCSGC	3 cut(s)	56, 59, 575
TfiI	GAWTC	2 cut(s)	799, 809
Tru1I	TTAA	6 cut(s)	860, 1440, 1449, 1524, 1724, 1937
Tru9I	TTAA	6 cut(s)	860, 1440, 1449, 1524, 1724, 1937
TscAI	CASTG	5 cut(s)	268, 442, 537, 1513, 1765
TseI	GCWGC	5 cut(s)	231, 921, 1058, 1916, 1931
TspDTI	ATGAA	6 cut(s)	380, 1225, 1365, 1631, 1656, 2013
TspGWI	ACGGA	2 cut(s)	93, 892
TspRI	CASTG	5 cut(s)	268, 442, 537, 1513, 1765
Van91I	CCANNNNNTGG	4 cut(s)	536, 646, 1199, 1870
XagI	CCTNNNNNAGG	1 cut(s)	1769
XceI	RCATGY	1 cut(s)	1208
XhoI	CTCGAG	1 cut(s)	1829
XmnI	GAANNNNTTC	3 cut(s)	827, 1232, 1392
XspI	CTAG	3 cut(s)	195, 989, 1347

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.