Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0007134

Physical Location & Seq

Genomic Coordinates Reverse (-)

45338197 .. 45345241

Gene Structure 7045 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025368

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGGCTAGCATGGCTCGGGCGAGCTCGGGGCTCCAATATCCTGAGCGATTCTACGCGGCGGCCTCTTACGCCGGCTTCGACGGATCTCCTCGCTCCTCCTCTAAAGCCGTTCGCGCCAAGTTCAACTCCGAGTCCGCTCTCATCCTCTACGCTCTCTACCAGCAGGCTACTGTAGGACCGTGTAACACACCAGAACCGAGTGCTTGGAATGCAGTTGAGAAAAGCAAATGGAGCAGCTGGAAGAAGCTTGCGGACATGGCCTCCACTGAAGCAATGCGTCTTTTTGTGAAGATATTGGAGGAAGAAGATCCAGGTTGGTATTCAAGAGCATCAAACTCTGTTTCTGAGCCTGTGGTAGATGTACAAATGAATCAAAATTCAAAAGTTGGGCCAGTTGTTGAGAATGGGAACTCTTATCCAGAGACAAAGACAATCTCAGCTGAAAATGGGAGCATACAGGAAGCACAGGATAAAGATGTTGTCTCTGAAGGCATTGGTTCTGTTGGTGTTTACGATCAGTGGATTGCACCTCCAGTATCTGGCCAACGTCCGAAAGCCCGATATGAGCATGGAACAGCAGTTACGCAAGACAAGATGTATATATATGGTGGAAACCACAATGGTCGTTACCTAAATGATCTCCATGTCTTCGATTTAAGGAGTTGGACTTGGTCAAAGATTGAGGCCAAAGTTGGGGCTGAATCACCCGACTCGCCGTCTCCAGTAACAATAAGCCCTTGTGCTGGTCATTCCTTGATACCATGGGGCAATAAACTTATCTCAATTGCTGGACATACAAAGGATCCTACTGAGTCTATTCAAGTAAAGGCATTCGATCTACACACTTCTACTTGGTCGTTCTTAAAGACTTATGGGAAACCACCGACATCACGCGGAGGTCAATCCGTCACCCTTATTGGAGGTAGCTTGGTGATTTTTGGTGGACAGGATGCGAAGAGAACTTTGTTAAATGATCTCCACATTCTTGACCTAGAAACAATGACATGGGATGAGATTGATGCTGTAGGGGTGTCCCCTTCTCCAAGGTCTGATCATGCTGCTGCTGTTCATGCTGACCGCTACCTCCTTATCTTTGGTGGGGGTTCCCATGCAACTTGCTTCAATGATCTGCATGTCCTTGATTTGCAAACTATGGAGTGGTCACGACCCACACAACAAGGCGAGATACCAACTCCACGGGCTGGACATGCAGGTGTGCCAGTTGGGGAAAATTGGTTCATTGTTGGTGGTGGTGACAATAAGAGTGGGGTTTCAGAAACTGTTGTCCTAAATATGTCTACACTAGTTTGGTCTGTTGTAACTACTGTTGAAGGGCGTATTCCTGTTGCTAGTGAGGGATTAAGTTTGGTCGTAGGTTCTTACAATGGTGAAGATGTGCTTTTATCTTTTGGAGGATACAATGGGCGTTACAACAATGAGGTTCACGTTCTCAAACCAAGCCACAAGTCAAGCTTGCAATCAAAAATGGAAACTTCTGTCCCTGACAGTGTCTCTGCTGTGCATAATGCCACTACTGCTACCAGAGATGTGGAGTCCGAGTATGGAGCTGGACAAGAAGGAAAAATTAGGGAAATAGTTATGGACAACGTTGACACAGAGGCAATGACTCTCCTGCAGAAATTCAGAGGCAAGAATTTCAATGAGCATCTTATAGCAACACTCAAAGGAGATAAAGAGGAATTAGAATCATCACTCAGTAAGGAGAAGGCGCAATCTCTCCAATTAAAACAAGAGTTATCAGAAGCAGACACTCGTAACACTGACCTATACAAGGAGCTTCAATCTGTACGGGGACAACTTGCATCTGAGCAGTCCAGATGCTTCAAACTTGAGGTTGAACTAGCAGAACTACGACAGAAGCTCCAATCAATGGAGGCATTGCAGAAGGAACTTGAACTCCTCCAGCGACAAAAAGCTGCATCTGAACAAGCTGCTCTGAATGCTAAAAAGCAGGGCTCAGGAGGCGTCTGGGGCTGGCTTGCCGGAACACCTGGCAGTGAAAAATCTGATGATGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000062 GO:0000166 GO:0001101 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006810 GO:0006869 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009753 GO:0010033 GO:0010876 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033218 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048037 GO:0050662 GO:0050896 GO:0051179 GO:0051234 GO:0071702 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901567 GO:1901681 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ACBP Kelch_3 Kelch_4 Kelch_5 Kelch_6

Protein Analysis

680

Amino Acids

73.89

Weight (kDa)

5.46

Isoelectric Point (pI)

44.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACBP	PF00887	17 - 98	9.6e-19	Acyl CoA binding protein
Kelch_HCF	PF13854	171 - 367	9.8e-22	Host cell factor, Kelch-repeats domain
Beta-prop_FBX42	PF13415	172 - 230	1.6e-11	FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1	PF24981	172 - 303	9.9e-19	Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7	PF24681	173 - 234	3.9e-16	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_TYW4	PF13418	184 - 374	6.6e-06	tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_1	PF01344	186 - 227	3.2e-09	Kelch motif
Beta-prop_FBX42	PF13415	198 - 368	4e-35	FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	249 - 458	6.1e-56	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1	PF24981	262 - 369	7.5e-14	Attractin/LZTR1 beta-propeller
Beta-prop_ATRN-LZTR1	PF24981	311 - 493	4.5e-21	Attractin/LZTR1 beta-propeller
Beta-prop_FBX42	PF13415	352 - 503	2.5e-22	FBX42, beta-propeller domain
Kelch_HCF	PF13854	361 - 417	3.6e-07	Host cell factor, Kelch-repeats domain
Kelch_HCF	PF13854	393 - 489	2.9e-06	Host cell factor, Kelch-repeats domain
ACBP4_C	PF24922	555 - 658	1.7e-40	Acyl-CoA-binding domain-containing protein 4-6, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007134)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05420 AT3G05420 AT5G27630 AT5G27630 AT5G27630 AT5G27630
fragaria_vesca	FvH4_6g06301 FvH4_6g06301 FvH4_6g06301
malus_domestica	MD04G1202500.v1.1 MD12G1216500.v1.1
prunus_persica	Prupe.6G322800_v2.0.a1 Prupe.6G322800_v2.0.a1
pyrus_communis	pycom04g17870 pycom12g20200
rosa_chinensis	RchiOBHm_Chr3g0455341
rosa_laevigata	RLG00000025368
rosa_multiflora	Rmu_sc0002169.1_g000045 Rmu_sc0024960.1_g000003
rosa_roxburghii	Rroxscaffold_6G00424350
rosa_rugosa	Rorug03G0006600 Rorug03G0006700
rosa_samantha	Rh3CG066800 Rh3DG068300
rosa_wichuraiana	Rw3G005220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1202
Acc36I	ACCTGC	1 cut(s)	1202
AccB7I	CCANNNNNTGG	3 cut(s)	539, 1202, 1891
AccBSI	CCGCTC	1 cut(s)	137
AccI	GTMKAC	1 cut(s)	1298
AccII	CGCG	3 cut(s)	56, 114, 894
AciI	CCGC	6 cut(s)	56, 59, 135, 251, 894, 1078
AclI	AACGTT	1 cut(s)	1608
AclWI	GGATC	4 cut(s)	91, 302, 797, 810
AcoI	YGGCCR	1 cut(s)	541
AcsI	RAATTY	3 cut(s)	376, 1640, 1654
AcuI	CTGAAG	2 cut(s)	288, 507
AcyI	GRCGYC	1 cut(s)	1986
AfaI	GTAC	2 cut(s)	363, 1809
AfiI	CCNNNNNNNGG	6 cut(s)	539, 693, 743, 1202, 1792, 1891
AhdI	GACNNNNNGTC	1 cut(s)	715
AhlI	ACTAGT	1 cut(s)	1303
AjnI	CCWGG	2 cut(s)	310, 2011
AloI	GAACNNNNNNTCC	4 cut(s)	1220, 1252, 1902, 1934
Alw21I	GWGCWC	1 cut(s)	26
Alw26I	GTCTC	4 cut(s)	416, 487, 723, 1516
AlwI	GGATC	4 cut(s)	91, 302, 797, 810
AlwNI	CAGNNNCTG	2 cut(s)	539, 1280
Ama87I	CYCGRG	2 cut(s)	15, 25
AoxI	GGCC	5 cut(s)	60, 258, 389, 541, 684
ApeKI	GCWGC	5 cut(s)	234, 1058, 1061, 1937, 1952
ApoI	RAATTY	3 cut(s)	376, 1640, 1654
AspLEI	GCGC	2 cut(s)	116, 1732
AspS9I	GGNCC	2 cut(s)	176, 389
AsuHPI	GGTGA	5 cut(s)	696, 901, 943, 1265, 1400
AsuNHI	GCTAGC	1 cut(s)	5
AvaI	CYCGRG	2 cut(s)	15, 25
AvaII	GGWCC	1 cut(s)	176
BalI	TGGCCA	1 cut(s)	543
BamHI	GGATCC	1 cut(s)	802
BanII	GRGCYC	3 cut(s)	26, 33, 1979
BbsI	GAAGAC	1 cut(s)	640
Bbv12I	GWGCWC	1 cut(s)	26
BbvI	GCAGC	5 cut(s)	246, 1045, 1048, 1924, 1939
BceAI	ACGGC	2 cut(s)	92, 700
BciT130I	CCWGG	2 cut(s)	312, 2013
BciVI	GTATCC	1 cut(s)	1409
BclI	TGATCA	1 cut(s)	1051
BcoDI	GTCTC	4 cut(s)	416, 487, 723, 1516
BcuI	ACTAGT	1 cut(s)	1303
BfaI	CTAG	5 cut(s)	6, 992, 1304, 1350, 1862
BfmI	CTRYAG	3 cut(s)	171, 1023, 1634
BfuAI	ACCTGC	1 cut(s)	1202
BfuI	GTATCC	1 cut(s)	1409
BisI	GCNGC	7 cut(s)	57, 60, 235, 1059, 1062, 1938, 1953
BlsI	GCNGC	7 cut(s)	58, 61, 236, 1060, 1063, 1939, 1954
Bme1390I	CCNGG	2 cut(s)	312, 2013
Bme18I	GGWCC	1 cut(s)	176
BmeRI	GACNNNNNGTC	1 cut(s)	715
BmeT110I	CYCGRG	2 cut(s)	15, 25
BmgT120I	GGNCC	2 cut(s)	176, 389
BmiI	GGNNCC	3 cut(s)	32, 804, 1105
BmrFI	CCNGG	2 cut(s)	312, 2013
BmsI	GCATC	8 cut(s)	338, 940, 1009, 1675, 1829, 1832, 1949, 2023
BmtI	GCTAGC	1 cut(s)	9
BpiI	GAAGAC	1 cut(s)	640
BpmI	CTGGAG	3 cut(s)	516, 705, 1907
Bpu10I	CCTNAGC	2 cut(s)	42, 1978
BpuEI	CTTGAG	1 cut(s)	1871
BsaHI	GRCGYC	1 cut(s)	1986
BsaJI	CCNNGG	3 cut(s)	761, 1043, 1196
BsaXI	ACNNNNNCTCC	8 cut(s)	245, 275, 1544, 1574, 1866, 1896, 1902, 1932
Bsc4I	CCNNNNNNNGG	6 cut(s)	539, 693, 743, 1202, 1792, 1891
Bse118I	RCCGGY	1 cut(s)	71
Bse1I	ACTGG	4 cut(s)	392, 533, 722, 1220
Bse3DI	GCAATG	3 cut(s)	279, 1629, 1898
BseBI	CCWGG	2 cut(s)	312, 2013
BseDI	CCNNGG	3 cut(s)	761, 1043, 1196
BseGI	GGATG	3 cut(s)	141, 955, 1015
BseLI	CCNNNNNNNGG	6 cut(s)	539, 693, 743, 1202, 1792, 1891
BseMI	GCAATG	3 cut(s)	279, 1629, 1898
BseMII	CTCAG	7 cut(s)	33, 336, 450, 801, 1729, 1818, 1992
BseNI	ACTGG	4 cut(s)	392, 533, 722, 1220
BseRI	GAGGAG	4 cut(s)	78, 85, 88, 1910
BseXI	GCAGC	5 cut(s)	246, 1045, 1048, 1924, 1939
Bsh1236I	CGCG	3 cut(s)	56, 114, 894
BshFI	GGCC	5 cut(s)	62, 260, 391, 543, 686
BsiHKAI	GWGCWC	1 cut(s)	26
BsiHKCI	CYCGRG	2 cut(s)	15, 25
BsiSI	CCGG	2 cut(s)	72, 2004
BslFI	GGGAC	3 cut(s)	1018, 1484, 1827
BslI	CCNNNNNNNGG	6 cut(s)	539, 693, 743, 1202, 1792, 1891
BsmAI	GTCTC	4 cut(s)	416, 487, 723, 1516
BsmBI	CGTCTC	1 cut(s)	723
BsmFI	GGGAC	3 cut(s)	1018, 1484, 1827
BsmI	GAATGC	3 cut(s)	214, 830, 1966
BsnI	GGCC	5 cut(s)	62, 260, 391, 543, 686
BsoBI	CYCGRG	2 cut(s)	15, 25
Bsp1286I	GDGCHC	3 cut(s)	26, 33, 1979
Bsp1407I	TGTACA	1 cut(s)	361
Bsp143I	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
Bsp19I	CCATGG	1 cut(s)	761
BspACI	CCGC	6 cut(s)	56, 59, 135, 251, 894, 1078
BspANI	GGCC	5 cut(s)	62, 260, 391, 543, 686
BspCNI	CTCAG	7 cut(s)	34, 337, 449, 802, 1728, 1819, 1991
BspFNI	CGCG	3 cut(s)	56, 114, 894
BspLI	GGNNCC	3 cut(s)	32, 804, 1105
BspMAI	CTGCAG	1 cut(s)	1638
BspMI	ACCTGC	1 cut(s)	1202
BspOI	GCTAGC	1 cut(s)	9
BspPI	GGATC	4 cut(s)	91, 302, 797, 810
BsrBI	CCGCTC	1 cut(s)	137
BsrDI	GCAATG	3 cut(s)	279, 1629, 1898
BsrFI	RCCGGY	1 cut(s)	71
BsrGI	TGTACA	1 cut(s)	361
BsrI	ACTGG	4 cut(s)	392, 533, 722, 1220
BssAI	RCCGGY	1 cut(s)	71
BssECI	CCNNGG	3 cut(s)	761, 1043, 1196
BssMI	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
BssNI	GRCGYC	1 cut(s)	1986
BssT1I	CCWWGG	2 cut(s)	761, 1043
Bst2UI	CCWGG	2 cut(s)	312, 2013
Bst4CI	ACNGT	5 cut(s)	172, 180, 1282, 1327, 1508
Bst6I	CTCTTC	1 cut(s)	950
BstACI	GRCGYC	1 cut(s)	1986
BstAUI	TGTACA	1 cut(s)	361
BstC8I	GCNNGC	8 cut(s)	7, 22, 73, 165, 249, 1475, 1997, 2001
BstDEI	CTNAG	7 cut(s)	42, 345, 436, 810, 1715, 1827, 1978
BstDSI	CCRYGG	2 cut(s)	761, 1196
BstENI	CCTNNNNNAGG	1 cut(s)	1790
BstF5I	GGATG	3 cut(s)	141, 955, 1015
BstFNI	CGCG	3 cut(s)	56, 114, 894
BstHHI	GCGC	2 cut(s)	116, 1732
BstKTI	GATC	9 cut(s)	86, 310, 517, 640, 805, 838, 976, 1054, 1129
BstMAI	GTCTC	4 cut(s)	416, 487, 723, 1516
BstMBI	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
BstNI	CCWGG	2 cut(s)	312, 2013
BstNSI	RCATGY	2 cut(s)	1136, 1211
BstSCI	CCNGG	2 cut(s)	310, 2011
BstSFI	CTRYAG	3 cut(s)	171, 1023, 1634
BstUI	CGCG	3 cut(s)	56, 114, 894
BstV1I	GCAGC	5 cut(s)	246, 1045, 1048, 1924, 1939
BstV2I	GAAGAC	1 cut(s)	640
BstX2I	RGATCY	3 cut(s)	83, 307, 802
BstXI	CCANNNNNNTGG	1 cut(s)	1549
BstYI	RGATCY	3 cut(s)	83, 307, 802
BsuI	GTATCC	1 cut(s)	1409
BsuRI	GGCC	5 cut(s)	62, 260, 391, 543, 686
BtgI	CCRYGG	2 cut(s)	761, 1196
BtsCI	GGATG	3 cut(s)	141, 955, 1015
BtsI	GCAGTG	1 cut(s)	2023
BtsIMutI	CAGTG	5 cut(s)	264, 524, 1513, 1779, 2023
BveI	ACCTGC	1 cut(s)	1202
Cac8I	GCNNGC	8 cut(s)	7, 22, 73, 165, 249, 1475, 1997, 2001
CaiI	CAGNNNCTG	2 cut(s)	539, 1280
CfoI	GCGC	2 cut(s)	116, 1732
Cfr10I	RCCGGY	1 cut(s)	71
Cfr13I	GGNCC	2 cut(s)	176, 389
CseI	GACGC	2 cut(s)	266, 1975
Csp6I	GTAC	2 cut(s)	362, 1808
CspCI	CAANNNNNGTGG	2 cut(s)	1246, 1281
CviQI	GTAC	2 cut(s)	362, 1808
DdeI	CTNAG	7 cut(s)	42, 345, 436, 810, 1715, 1827, 1978
DpnI	GATC	9 cut(s)	85, 309, 516, 639, 804, 837, 975, 1053, 1128
DpnII	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
DriI	GACNNNNNGTC	1 cut(s)	715
EaeI	YGGCCR	1 cut(s)	541
Eam1104I	CTCTTC	1 cut(s)	950
Eam1105I	GACNNNNNGTC	1 cut(s)	715
EarI	CTCTTC	1 cut(s)	950
Ecl136II	GAGCTC	1 cut(s)	24
Eco130I	CCWWGG	2 cut(s)	761, 1043
Eco24I	GRGCYC	3 cut(s)	26, 33, 1979
Eco47I	GGWCC	1 cut(s)	176
Eco53kI	GAGCTC	1 cut(s)	24
Eco57I	CTGAAG	2 cut(s)	288, 507
Eco88I	CYCGRG	2 cut(s)	15, 25
EcoICRI	GAGCTC	1 cut(s)	24
EcoNI	CCTNNNNNAGG	1 cut(s)	1790
EcoRII	CCWGG	2 cut(s)	310, 2011
EcoT14I	CCWWGG	2 cut(s)	761, 1043
EcoT38I	GRGCYC	3 cut(s)	26, 33, 1979
ErhI	CCWWGG	2 cut(s)	761, 1043
Esp3I	CGTCTC	1 cut(s)	723
FalI	AAGNNNNNCTT	4 cut(s)	1383, 1415, 1457, 1489
FaqI	GGGAC	3 cut(s)	1018, 1484, 1827
FbaI	TGATCA	1 cut(s)	1051
FblI	GTMKAC	1 cut(s)	1298
Fnu4HI	GCNGC	7 cut(s)	57, 60, 235, 1059, 1062, 1938, 1953
FokI	GGATG	3 cut(s)	128, 962, 1022
FriOI	GRGCYC	3 cut(s)	26, 33, 1979
Fsp4HI	GCNGC	7 cut(s)	57, 60, 235, 1059, 1062, 1938, 1953
FspBI	CTAG	5 cut(s)	6, 992, 1304, 1350, 1862
GlaI	GCGC	2 cut(s)	115, 1731
GluI	GCNGC	7 cut(s)	57, 60, 235, 1059, 1062, 1938, 1953
GsuI	CTGGAG	3 cut(s)	516, 705, 1907
HaeIII	GGCC	5 cut(s)	62, 260, 391, 543, 686
HapII	CCGG	2 cut(s)	72, 2004
HgaI	GACGC	2 cut(s)	266, 1975
HhaI	GCGC	2 cut(s)	116, 1732
Hin1I	GRCGYC	1 cut(s)	1986
Hin6I	GCGC	2 cut(s)	114, 1730
HinP1I	GCGC	2 cut(s)	114, 1730
HincII	GTYRAC	1 cut(s)	1612
HindII	GTYRAC	1 cut(s)	1612
HindIII	AAGCTT	2 cut(s)	245, 1471
HinfI	GANTC	9 cut(s)	48, 131, 370, 701, 710, 812, 1553, 1627, 1706
HpaII	CCGG	2 cut(s)	72, 2004
HphI	GGTGA	5 cut(s)	696, 901, 943, 1265, 1400
Hpy166II	GTNNAC	5 cut(s)	511, 944, 1299, 1444, 1612
Hpy188III	TCNNGA	7 cut(s)	41, 324, 419, 986, 1164, 1836, 1980
Hpy8I	GTNNAC	5 cut(s)	511, 944, 1299, 1444, 1612
Hpy99I	CGWCG	1 cut(s)	83
HpyAV	CCTTC	6 cut(s)	482, 1047, 1325, 1571, 1720, 1900
HpyCH4III	ACNGT	5 cut(s)	172, 180, 1282, 1327, 1508
HpyCH4IV	ACGT	3 cut(s)	547, 1446, 1608
HpyF3I	CTNAG	7 cut(s)	42, 345, 436, 810, 1715, 1827, 1978
HpySE526I	ACGT	3 cut(s)	547, 1446, 1608
Hsp92I	GRCGYC	1 cut(s)	1986
HspAI	GCGC	2 cut(s)	114, 1730
KroI	GCCGGC	1 cut(s)	71
KroNI	GCCGGC	1 cut(s)	73
Ksp22I	TGATCA	1 cut(s)	1051
Kzo9I	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
LmnI	GCTCC	7 cut(s)	36, 98, 231, 450, 1565, 1795, 1887
Lsp1109I	GCAGC	5 cut(s)	246, 1045, 1048, 1924, 1939
LweI	GCATC	8 cut(s)	338, 940, 1009, 1675, 1829, 1832, 1949, 2023
MaeI	CTAG	5 cut(s)	6, 992, 1304, 1350, 1862
MaeII	ACGT	3 cut(s)	547, 1446, 1608
MalI	GATC	9 cut(s)	85, 309, 516, 639, 804, 837, 975, 1053, 1128
MbiI	CCGCTC	1 cut(s)	137
MboI	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
MboII	GAAGA	7 cut(s)	253, 301, 314, 317, 640, 967, 1403
MfeI	CAATTG	1 cut(s)	783
MflI	RGATCY	3 cut(s)	83, 307, 802
MhlI	GDGCHC	3 cut(s)	26, 33, 1979
MlsI	TGGCCA	1 cut(s)	543
MluCI	AATT	8 cut(s)	376, 783, 1231, 1584, 1640, 1654, 1700, 1742
MluNI	TGGCCA	1 cut(s)	543
MlyI	GAGTC	5 cut(s)	140, 704, 821, 1562, 1621
MmeI	TCCRAC	1 cut(s)	644
Mox20I	TGGCCA	1 cut(s)	543
MroNI	GCCGGC	1 cut(s)	71
MscI	TGGCCA	1 cut(s)	543
MseI	TTAA	5 cut(s)	656, 863, 968, 1361, 1745
MslI	CAYNNNNRTG	1 cut(s)	1212
Msp20I	TGGCCA	1 cut(s)	543
MspA1I	CMGCKG	2 cut(s)	237, 440
MspI	CCGG	2 cut(s)	72, 2004
MspR9I	CCNGG	2 cut(s)	312, 2013
MunI	CAATTG	1 cut(s)	783
Mva1269I	GAATGC	3 cut(s)	214, 830, 1966
MvaI	CCWGG	2 cut(s)	312, 2013
MvnI	CGCG	3 cut(s)	56, 114, 894
NaeI	GCCGGC	1 cut(s)	73
NcoI	CCATGG	1 cut(s)	761
NdeII	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
NgoMIV	GCCGGC	1 cut(s)	71
NheI	GCTAGC	1 cut(s)	5
NlaIV	GGNNCC	3 cut(s)	32, 804, 1105
NmuCI	GTSAC	3 cut(s)	907, 1161, 1253
NspI	RCATGY	2 cut(s)	1136, 1211
PaqCI	CACCTGC	1 cut(s)	1202
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	3 cut(s)	214, 830, 1966
PdiI	GCCGGC	1 cut(s)	73
PfeI	GAWTC	4 cut(s)	48, 370, 701, 1706
PflFI	GACNNNGTC	2 cut(s)	670, 1508
PflMI	CCANNNNNTGG	3 cut(s)	539, 1202, 1891
PkrI	GCNGC	7 cut(s)	58, 61, 236, 1060, 1063, 1939, 1954
PleI	GAGTC	5 cut(s)	139, 704, 820, 1561, 1621
PpsI	GAGTC	5 cut(s)	139, 704, 820, 1561, 1621
Psp124BI	GAGCTC	1 cut(s)	26
Psp1406I	AACGTT	1 cut(s)	1608
Psp6I	CCWGG	2 cut(s)	310, 2011
PspGI	CCWGG	2 cut(s)	310, 2011
PspN4I	GGNNCC	3 cut(s)	32, 804, 1105
PspPI	GGNCC	2 cut(s)	176, 389
PsrI	GAACNNNNNNTAC	2 cut(s)	565, 597
PstI	CTGCAG	1 cut(s)	1638
PstNI	CAGNNNCTG	2 cut(s)	539, 1280
PsuI	RGATCY	3 cut(s)	83, 307, 802
PsyI	GACNNNGTC	2 cut(s)	670, 1508
PvuII	CAGCTG	2 cut(s)	237, 440
RsaI	GTAC	2 cut(s)	363, 1809
RsaNI	GTAC	2 cut(s)	362, 1808
RseI	CAYNNNNRTG	1 cut(s)	1212
SacI	GAGCTC	1 cut(s)	26
SaqAI	TTAA	5 cut(s)	656, 863, 968, 1361, 1745
SatI	GCNGC	7 cut(s)	57, 60, 235, 1059, 1062, 1938, 1953
Sau3AI	GATC	9 cut(s)	83, 307, 514, 637, 802, 835, 973, 1051, 1126
Sau96I	GGNCC	2 cut(s)	176, 389
SchI	GAGTC	5 cut(s)	140, 704, 821, 1562, 1621
ScrFI	CCNGG	2 cut(s)	312, 2013
SduI	GDGCHC	3 cut(s)	26, 33, 1979
SfaNI	GCATC	8 cut(s)	338, 940, 1009, 1675, 1829, 1832, 1949, 2023
SfcI	CTRYAG	3 cut(s)	171, 1023, 1634
SinI	GGWCC	1 cut(s)	176
SmiMI	CAYNNNNRTG	1 cut(s)	1212
SmlI	CTYRAG	1 cut(s)	1850
SmoI	CTYRAG	1 cut(s)	1850
SpeI	ACTAGT	1 cut(s)	1303
Sse9I	AATT	8 cut(s)	376, 783, 1231, 1584, 1640, 1654, 1700, 1742
SsiI	CCGC	6 cut(s)	56, 59, 135, 251, 894, 1078
SspMI	CTAG	5 cut(s)	6, 992, 1304, 1350, 1862
SstI	GAGCTC	1 cut(s)	26
StyD4I	CCNGG	2 cut(s)	310, 2011
StyI	CCWWGG	2 cut(s)	761, 1043
TaaI	ACNGT	5 cut(s)	172, 180, 1282, 1327, 1508
TaiI	ACGT	3 cut(s)	550, 1449, 1611
TaqI	TCGA	3 cut(s)	78, 651, 834
TasI	AATT	8 cut(s)	376, 783, 1231, 1584, 1640, 1654, 1700, 1742
TatI	WGTACW	1 cut(s)	361
TauI	GCSGC	2 cut(s)	59, 62
TfiI	GAWTC	4 cut(s)	48, 370, 701, 1706
Tru1I	TTAA	5 cut(s)	656, 863, 968, 1361, 1745
Tru9I	TTAA	5 cut(s)	656, 863, 968, 1361, 1745
TscAI	CASTG	5 cut(s)	271, 524, 1513, 1786, 2023
TseFI	GTSAC	3 cut(s)	907, 1161, 1253
TseI	GCWGC	5 cut(s)	234, 1058, 1061, 1937, 1952
Tsp45I	GTSAC	3 cut(s)	907, 1161, 1253
TspDTI	ATGAA	3 cut(s)	383, 1058, 1228
TspGWI	ACGGA	2 cut(s)	96, 895
TspRI	CASTG	5 cut(s)	271, 524, 1513, 1786, 2023
Tth111I	GACNNNGTC	2 cut(s)	670, 1508
Van91I	CCANNNNNTGG	3 cut(s)	539, 1202, 1891
VpaK11BI	GGWCC	1 cut(s)	176
XagI	CCTNNNNNAGG	1 cut(s)	1790
XapI	RAATTY	3 cut(s)	376, 1640, 1654
XceI	RCATGY	2 cut(s)	1136, 1211
XmiI	GTMKAC	1 cut(s)	1298
XspI	CTAG	5 cut(s)	6, 992, 1304, 1350, 1862

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.