FvH4_6g06310

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010008
Physical Location & Seq
Forward (+)
3635274 .. 3640041
4768 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g06310.t2

Sequence Viewer

Length: 3033 bp
ATGAACAACGATTTCGAATCCGACGCGAAATCCGATTCCGGAGACGCCGGTAAGGCTTTGAACGGCGGCGCCGGTATCTCCGAGGATGATGCTAGGGTTTTGCCGACGGAGGAGAGCGAGGGCGGCGGGAGATCTGAGGAGGATAAGGTTAGGGTTAGCGAGGAGAGTGGCGGTGGAAATGTAGATAAGGTAACGGAGTCGAATCCGAGCGGCGGCGAGGGGTTGGATTACGACGAAGAGGATGACGTGTTTGATATCGGGAGGGTTGAGCTTGATGAGGATCAGGAGGAGGATAGCTCCGAGGCTCAGAACGATGGGGATAGAGATGAGAGTGTGGATTTGTTATCGGAGTTTGATGAGTTTGTGGCTAATGAGAAGGACGGTATGGCTTTAGGGACTTCCAGAGCTCTGAGCTACGGTTTCCAAGTCGGAGACATGGTGTGGGGGAAGGTGAAGTCTCATCCGTGGTGGCCAGGGCATATATTCAATGAGGCCTTCGCTACGTCTCAGGTGCGGCGGACTAGAAGAGAAGGTCATGTCTTGGTGGCCTTCTTTGGTGATAGTAGCTACGGCTGGTTCGACCCGGCTGAGCTTATCCCATTCGAGCCTCATTTTGCTGAGAAGTCTCGGCAGACGAATTACAGGAATTTCGCCAGGGCTGTGGAGGAGGCTGTGGATGAGGCGAGCCGGAGGTGTGGCGTTGGGTTTGTGTGTAAATGTAGGAATCCGTATAATTTTCGGGGTACTAGTGTGCCTGGGTACTTTGTTGTTGATGTGCCGGATTATGAGCATGGGGCGGTTTATTCTACGGATCAGATTAAGAAGGCCAGGGATGGTTTTAATCCTGCTGAGCTTGTGTCTCTGGTAAAGCAGTTGGCGAAGTCACCAGTCCAAGGGGATCAGAAGAGCCTTAGCTTCATTAAGAATAAGGCTACTATGTTTGCTTATCGGAAGGCAGTGTTTGAGGAGTATGATGAGACCTATGCTCAGGCATTTGGGGCTCGGTCTTCACGACCTGCTGTGCCAGATCAGCCTGTTAAGCCTCGAGCACCTTTGAGCGGTCCTTTGGTGATTGCTGAAGTCCTGGGTGGACGGAAGAGTGCCACAAAACCCATGAAAGTCAAGGACCATTCAAAAAAAGACAAATACCTTTTCAAGCGGAGAGATGAAGCTAGTAACGTGAAACCCCACCAAACTACGCAGGGCCAAGCTAGTTCCTCAGCCGCATCTACTTACTTGGAGGGATCAGTAGCATTAGGAGATGGAGACTATAAGTTGCAAAAGAGGGCACCGTCTATCTCTATGAAGCCTCAGGTTTTAAAACATGAGCAGACTGAGAATATGAGCAGGGATGCTTCTGGAAAAGAACCTGTAAACATAAATCAGGTACCAGCAAACAGCTCTGTTGCCAGCCAAGGTGTTACCACCGGTTCAAAGCTATCTCTGAAGCTTTCTTTTGACAAGGAAACTGGAGCCCTGCAGGAAGTGAAAGATGCATTGACACAGAATGTAGCTGAGGGTCATTCAAGCACAGGTCACTCTGAATTATTCAGTCAGGGGACCAAGCAATGTATAAAAGATGAACCATCACAATCTTTGAAGCAGGAAGGGGAGGGTCCAATGGAGGTTGAAGGGAGTGCCAAATTGTCTGGGTTAAAGGAAGACAATGAATTGTCTGGTCACACTGTTGGAGATTCTTCACTGATCGAGGCAAAGAGTTCAGCTGGAAAGAAGGCTGTTGGTGGAGTGAAAAAGGCAAAGTTTCTTAAACGGCCACGGGGTGACATGAACCCTGCCATATCTGTTATGGAAGATAAAAAGAAAAAGAAGAAGAAGAGACAGTTAGGTTCCGATATAGGCTTCAGAGATCCACAGAGGATTGTGACTTCCGGAAAAGTGGGTTCCGTGGTGGATAGGGATGCAGGAAATGACAATCATGCAGGTTTGTCTCCAGAGGAAGATTTCAAGGTAGAACATCATAAGAAAGATGTTACTGTCAAGAAGGCCTTGTCTGAATCTGCTGGATTGTTGCCAATTCTTACAGAGGTTGAATTACCGCAACTAGTAAGCGATTTGCAAGCCCTTGCCCTTGATCCTTTTCATGGTCGTGAAACAAACAACCCTACCATTGTTCGGCAGTTCTTTCTGCAATTCCGGGCCCTCGTCTATCAGAAAAGCTTGGTCCTGTCACCACCATCAGAGACAGAGCCTCTTGAAGGTCACATCGCTAAAAATCCATCTGGTGTCAAAACCTCAGAAATTTCTCCTCCGGAACCAGTAAGAGATGTTCCCTCCTCGAAGTCGGCAAAACCCTTGTTCAGATCTGGTGATCGCACCATAGCTGGGCGGAAACGTGCTCCGTCCGATCGTCAAGGAGAGATTGCAGCTAAGAAGTCAAAGAAAATGAGTGATTTAAAATTATTGCATGCAGAGAGGAAGATTGGGCAAAAAAGTCAAGAGACCCAGCGTGGAGAGGTAAAAGAATCAGCGGTGCCCATTCCACGGAGGGCACCAAAGCCAGGTCTTGTGAAGAAAATGGAACCTCCGTCCAAGGTTGTTGAGCCCACCATGCTCGTGATGAAATTCCCTCCCACAATCTCTCTGCCTTCACCAGCAGAACTGAAGGCAAAGTTTGCTCGTTTTGGACCTACGGATCAGTCTGGTCTCCGTGTGTTCTACAAGTCCTCAACATGTCGAGTTGTATTTCTGTACAAATCTGATGCACAAGCAGCATTTAAGTTTGCAAGTTCAAACAAATCCTTTCTTGGCAACGTCAATGTGAGGTTCCAGCTTCGAGAGGTGGACGGTCCTGAAGTTCCTGCATCTGGGAAGGGTTATGGAGATGACAATTCTACTGAGACTCCGCGCGCTAAGGATTCTGCATTTATGCCAACTCCGGCACTCAAACAGCGGCAGCAGCAGTCTCTCTCACACAGTGCAGTCCAGCCGAAGTCGATTCTGAAGAAGTCATCGGGTGACGAGCCTCGAGGACAGATGATGGTGCACCTTCTTCTTCTACTTCTGTTGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
PWWP

Protein Analysis

1011

Amino Acids

110.28

Weight (kDa)

7.94

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 144 - 228 1.3e-18 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 2985
Acc36I ACCTGC 2 cut(s) 1024, 1931
Acc65I GGTACC 1 cut(s) 1387
AccB1I GGYRCC 5 cut(s) 68, 1288, 1387, 2491, 2509
AccBSI CCGCTC 2 cut(s) 210, 1059
AccII CGCG 3 cut(s) 26, 2866, 2868
AccIII TCCGGA 3 cut(s) 38, 1889, 2269
AclWI GGATC 7 cut(s) 288, 819, 906, 1252, 1862, 2087, 2661
AcoI YGGCCR 2 cut(s) 470, 1772
AcsI RAATTY 3 cut(s) 646, 2259, 2582
AcuI CTGAAG 6 cut(s) 1098, 1466, 1846, 2642, 2832, 2981
AcyI GRCGYC 2 cut(s) 45, 69
AdeI CACNNNGTG 2 cut(s) 1781, 2936
AfaI GTAC 4 cut(s) 745, 761, 1389, 2711
AflIII ACRYGT 2 cut(s) 246, 2690
AgeI ACCGGT 1 cut(s) 1427
AhlI ACTAGT 2 cut(s) 746, 2062
AjiI CACGTC 1 cut(s) 247
AjnI CCWGG 6 cut(s) 472, 653, 754, 827, 1083, 2518
Alw21I GWGCWC 4 cut(s) 409, 1051, 2359, 3006
Alw44I GTGCAC 1 cut(s) 3002
AlwI GGATC 7 cut(s) 288, 819, 906, 1252, 1862, 2087, 2661
Ama87I CYCGRG 2 cut(s) 1044, 2985
Aor13HI TCCGGA 3 cut(s) 38, 1889, 2269
AoxI GGCC 8 cut(s) 470, 492, 546, 825, 1204, 1772, 2004, 2157
ApaI GGGCCC 1 cut(s) 2161
ApaLI GTGCAC 1 cut(s) 3002
ApeKI GCWGC 4 cut(s) 2384, 2729, 2914, 2917
ApoI RAATTY 3 cut(s) 646, 2259, 2582
AsiGI ACCGGT 1 cut(s) 1427
Asp718I GGTACC 1 cut(s) 1387
AspLEI GCGC 3 cut(s) 71, 2868, 2870
AsuC2I CCSGG 2 cut(s) 584, 2156
AsuHPI GGTGA 9 cut(s) 463, 569, 876, 1081, 1793, 2181, 2339, 2601, 2987
AsuII TTCGAA 1 cut(s) 15
AvaI CYCGRG 2 cut(s) 1044, 2985
AvaII GGWCC 7 cut(s) 1061, 1126, 1560, 1616, 2182, 2645, 2807
AxyI CCTNAGG 1 cut(s) 1311
BaeGI GKGCMC 5 cut(s) 1291, 2161, 2496, 2512, 3006
BaeI ACNNNNGTAYC 2 cut(s) 735, 768
BalI TGGCCA 1 cut(s) 472
BanI GGYRCC 5 cut(s) 68, 1288, 1387, 2491, 2509
BanII GRGCYC 5 cut(s) 409, 1003, 1477, 2161, 2565
BauI CACGAG 1 cut(s) 2573
BbsI GAAGAC 2 cut(s) 999, 1668
Bbv12I GWGCWC 4 cut(s) 409, 1051, 2359, 3006
BbvCI CCTCAGC 2 cut(s) 1219, 1515
BbvI GCAGC 4 cut(s) 2396, 2741, 2926, 2929
BccI CCATC 7 cut(s) 308, 827, 1256, 1594, 2203, 2245, 2992
BceAI ACGGC 3 cut(s) 79, 586, 1787
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 6 cut(s) 474, 655, 756, 829, 1085, 2520
BcnI CCSGG 2 cut(s) 584, 2156
BcuI ACTAGT 2 cut(s) 746, 2062
BfaI CTAG 6 cut(s) 93, 522, 747, 1173, 1212, 2063
BfmI CTRYAG 1 cut(s) 1478
BfoI RGCGCY 1 cut(s) 72
BfuAI ACCTGC 2 cut(s) 1024, 1931
BglI GCCNNNNNGGC 1 cut(s) 53
BglII AGATCT 2 cut(s) 131, 2321
BlpI GCTNAGC 2 cut(s) 588, 849
Bme1390I CCNGG 8 cut(s) 474, 584, 655, 756, 829, 1085, 2156, 2520
Bme18I GGWCC 7 cut(s) 1061, 1126, 1560, 1616, 2182, 2645, 2807
BmeT110I CYCGRG 2 cut(s) 1044, 2985
BmgBI CACGTC 1 cut(s) 247
BmrFI CCNGG 8 cut(s) 474, 584, 655, 756, 829, 1085, 2156, 2520
BmsI GCATC 7 cut(s) 79, 1235, 1342, 1483, 1909, 2710, 2831
BpiI GAAGAC 2 cut(s) 999, 1668
BplI GAGNNNNNCTC 2 cut(s) 281, 313
BpmI CTGGAG 2 cut(s) 1491, 1935
Bpu10I CCTNAGC 5 cut(s) 911, 987, 1219, 1515, 2871
Bpu1102I GCTNAGC 2 cut(s) 588, 849
Bpu14I TTCGAA 1 cut(s) 15
BpuMI CCSGG 2 cut(s) 584, 2156
BsaBI GATNNNNATC 1 cut(s) 279
BsaHI GRCGYC 2 cut(s) 45, 69
BsaI GGTCTC 3 cut(s) 971, 2453, 2669
BsaWI WCCGGW 4 cut(s) 38, 1427, 1889, 2269
BsaXI ACNNNNNCTCC 4 cut(s) 423, 453, 2845, 2875
Bse118I RCCGGY 3 cut(s) 47, 71, 1427
Bse1I ACTGG 3 cut(s) 887, 1474, 2276
Bse21I CCTNAGG 1 cut(s) 1311
Bse3DI GCAATG 1 cut(s) 1574
Bse8I GATNNNNATC 1 cut(s) 279
BseAI TCCGGA 3 cut(s) 38, 1889, 2269
BseBI CCWGG 6 cut(s) 474, 655, 756, 829, 1085, 2520
BseGI GGATG 7 cut(s) 91, 247, 460, 682, 838, 1357, 1924
BseJI GATNNNNATC 1 cut(s) 279
BseMI GCAATG 1 cut(s) 1574
BseNI ACTGG 3 cut(s) 887, 1474, 2276
BsePI GCGCGC 1 cut(s) 2866
BseRI GAGGAG 8 cut(s) 125, 152, 176, 302, 680, 980, 2256, 2284
BseSI GKGCMC 5 cut(s) 1291, 2161, 2496, 2512, 3006
BseXI GCAGC 4 cut(s) 2396, 2741, 2926, 2929
BseYI CCCAGC 2 cut(s) 2342, 2463
BsgI GTGCAG 1 cut(s) 2958
Bsh1236I CGCG 3 cut(s) 26, 2866, 2868
Bsh1285I CGRYCG 1 cut(s) 2368
BshFI GGCC 8 cut(s) 472, 494, 548, 827, 1206, 1774, 2006, 2159
BshNI GGYRCC 5 cut(s) 68, 1288, 1387, 2491, 2509
BshTI ACCGGT 1 cut(s) 1427
BsiEI CGRYCG 1 cut(s) 2368
BsiHKAI GWGCWC 4 cut(s) 409, 1051, 2359, 3006
BsiHKCI CYCGRG 2 cut(s) 1044, 2985
BslFI GGGAC 2 cut(s) 409, 1573
BsmBI CGTCTC 2 cut(s) 36, 510
BsmFI GGGAC 2 cut(s) 409, 1573
BsnI GGCC 8 cut(s) 472, 494, 548, 827, 1206, 1774, 2006, 2159
Bso31I GGTCTC 3 cut(s) 971, 2453, 2669
BsoBI CYCGRG 2 cut(s) 1044, 2985
Bsp119I TTCGAA 1 cut(s) 15
Bsp120I GGGCCC 1 cut(s) 2157
Bsp13I TCCGGA 3 cut(s) 38, 1889, 2269
Bsp1407I TGTACA 1 cut(s) 2709
Bsp1720I GCTNAGC 2 cut(s) 588, 849
BspANI GGCC 8 cut(s) 472, 494, 548, 827, 1206, 1774, 2006, 2159
BspEI TCCGGA 3 cut(s) 38, 1889, 2269
BspFNI CGCG 3 cut(s) 26, 2866, 2868
BspMAI CTGCAG 1 cut(s) 1482
BspMI ACCTGC 2 cut(s) 1024, 1931
BspPI GGATC 7 cut(s) 288, 819, 906, 1252, 1862, 2087, 2661
BspQI GCTCTTC 1 cut(s) 899
BspT104I TTCGAA 1 cut(s) 15
BspT107I GGYRCC 5 cut(s) 68, 1288, 1387, 2491, 2509
BspTNI GGTCTC 3 cut(s) 971, 2453, 2669
BsrBI CCGCTC 2 cut(s) 210, 1059
BsrDI GCAATG 1 cut(s) 1574
BsrFI RCCGGY 3 cut(s) 47, 71, 1427
BsrGI TGTACA 1 cut(s) 2709
BsrI ACTGG 3 cut(s) 887, 1474, 2276
BssAI RCCGGY 3 cut(s) 47, 71, 1427
BssHII GCGCGC 1 cut(s) 2866
BssNI GRCGYC 2 cut(s) 45, 69
BssSI CACGAG 1 cut(s) 2573
BssT1I CCWWGG 3 cut(s) 892, 1414, 2550
Bst2BI CACGAG 1 cut(s) 2573
Bst2UI CCWGG 6 cut(s) 474, 655, 756, 829, 1085, 2520
Bst4CI ACNGT 8 cut(s) 383, 419, 1293, 1687, 1842, 1996, 2807, 2936
Bst6I CTCTTC 5 cut(s) 231, 520, 899, 1091, 1829
BstACI GRCGYC 2 cut(s) 45, 69
BstAPI GCANNNNNTGC 1 cut(s) 2633
BstAUI TGTACA 1 cut(s) 2709
BstBI TTCGAA 1 cut(s) 15
BstC8I GCNNGC 5 cut(s) 685, 1411, 2079, 2427, 2868
BstDSI CCRYGG 4 cut(s) 464, 1775, 1905, 2501
BstENI CCTNNNNNAGG 1 cut(s) 2214
BstF5I GGATG 7 cut(s) 91, 247, 460, 682, 838, 1357, 1924
BstFNI CGCG 3 cut(s) 26, 2866, 2868
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 3 cut(s) 71, 2868, 2870
BstMCI CGRYCG 1 cut(s) 2368
BstMWI GCNNNNNNNGC 9 cut(s) 53, 123, 998, 1030, 1039, 2569, 2633, 2729, 2917
BstNI CCWGG 6 cut(s) 474, 655, 756, 829, 1085, 2520
BstNSI RCATGY 2 cut(s) 2429, 2694
BstSCI CCNGG 8 cut(s) 472, 582, 653, 754, 827, 1083, 2154, 2518
BstSFI CTRYAG 1 cut(s) 1478
BstSLI GKGCMC 5 cut(s) 1291, 2161, 2496, 2512, 3006
BstUI CGCG 3 cut(s) 26, 2866, 2868
BstV1I GCAGC 4 cut(s) 2396, 2741, 2926, 2929
BstV2I GAAGAC 2 cut(s) 999, 1668
BstX2I RGATCY 3 cut(s) 131, 1867, 2321
BstXI CCANNNNNNTGG 1 cut(s) 661
BstYI RGATCY 3 cut(s) 131, 1867, 2321
Bsu36I CCTNAGG 1 cut(s) 1311
BsuRI GGCC 8 cut(s) 472, 494, 548, 827, 1206, 1774, 2006, 2159
BtgI CCRYGG 4 cut(s) 464, 1775, 1905, 2501
BtgZI GCGATG 1 cut(s) 2209
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 7 cut(s) 91, 247, 460, 682, 838, 1357, 1924
BtsI GCAGTG 1 cut(s) 963
BtsIMutI CAGTG 4 cut(s) 963, 1683, 1700, 2941
BveI ACCTGC 2 cut(s) 1024, 1931
Cac8I GCNNGC 5 cut(s) 685, 1411, 2079, 2427, 2868
CfoI GCGC 3 cut(s) 71, 2868, 2870
Cfr10I RCCGGY 3 cut(s) 47, 71, 1427
CseI GACGC 2 cut(s) 32, 53
Csp6I GTAC 4 cut(s) 744, 760, 1388, 2710
CspAI ACCGGT 1 cut(s) 1427
CviQI GTAC 4 cut(s) 744, 760, 1388, 2710
DinI GGCGCC 1 cut(s) 70
DraI TTTAAA 2 cut(s) 1320, 2415
DraIII CACNNNGTG 2 cut(s) 1781, 2936
EaeI YGGCCR 2 cut(s) 470, 1772
Eam1104I CTCTTC 5 cut(s) 231, 520, 899, 1091, 1829
EarI CTCTTC 5 cut(s) 231, 520, 899, 1091, 1829
EciI GGCGGA 2 cut(s) 532, 2362
Ecl136II GAGCTC 1 cut(s) 407
Eco130I CCWWGG 3 cut(s) 892, 1414, 2550
Eco147I AGGCCT 2 cut(s) 494, 2006
Eco24I GRGCYC 5 cut(s) 409, 1003, 1477, 2161, 2565
Eco31I GGTCTC 3 cut(s) 971, 2453, 2669
Eco32I GATATC 1 cut(s) 256
Eco47I GGWCC 7 cut(s) 1061, 1126, 1560, 1616, 2182, 2645, 2807
Eco53kI GAGCTC 1 cut(s) 407
Eco57I CTGAAG 6 cut(s) 1098, 1466, 1846, 2642, 2832, 2981
Eco81I CCTNAGG 1 cut(s) 1311
Eco88I CYCGRG 2 cut(s) 1044, 2985
EcoICRI GAGCTC 1 cut(s) 407
EcoNI CCTNNNNNAGG 1 cut(s) 2214
EcoO109I RGGNCCY 1 cut(s) 2158
EcoRII CCWGG 6 cut(s) 472, 653, 754, 827, 1083, 2518
EcoRV GATATC 1 cut(s) 256
EcoT14I CCWWGG 3 cut(s) 892, 1414, 2550
EcoT22I ATGCAT 1 cut(s) 1498
EcoT38I GRGCYC 5 cut(s) 409, 1003, 1477, 2161, 2565
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
ErhI CCWWGG 3 cut(s) 892, 1414, 2550
Esp3I CGTCTC 2 cut(s) 36, 510
FalI AAGNNNNNCTT 2 cut(s) 1991, 2023
FaqI GGGAC 2 cut(s) 409, 1573
FauI CCCGC 1 cut(s) 119
FokI GGATG 7 cut(s) 98, 254, 447, 689, 845, 1364, 1931
FriOI GRGCYC 5 cut(s) 409, 1003, 1477, 2161, 2565
FspBI CTAG 6 cut(s) 93, 522, 747, 1173, 1212, 2063
GlaI GCGC 3 cut(s) 70, 2867, 2869
GsaI CCCAGC 2 cut(s) 2346, 2467
GsuI CTGGAG 2 cut(s) 1491, 1935
HaeII RGCGCY 1 cut(s) 72
HaeIII GGCC 8 cut(s) 472, 494, 548, 827, 1206, 1774, 2006, 2159
HgaI GACGC 2 cut(s) 32, 53
HhaI GCGC 3 cut(s) 71, 2868, 2870
Hin1I GRCGYC 2 cut(s) 45, 69
Hin6I GCGC 3 cut(s) 69, 2866, 2868
HinP1I GCGC 3 cut(s) 69, 2866, 2868
HindIII AAGCTT 2 cut(s) 1448, 2176
HphI GGTGA 9 cut(s) 463, 569, 876, 1081, 1793, 2181, 2339, 2601, 2987
Hpy166II GTNNAC 4 cut(s) 1091, 1375, 2803, 3004
Hpy8I GTNNAC 4 cut(s) 1091, 1375, 2803, 3004
Hpy99I CGWCG 3 cut(s) 26, 109, 236
HpyCH4III ACNGT 8 cut(s) 383, 419, 1293, 1687, 1842, 1996, 2807, 2936
HpyCH4IV ACGT 5 cut(s) 246, 503, 1179, 2353, 2772
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 123, 998, 1030, 1039, 2569, 2633, 2729, 2917
HpySE526I ACGT 5 cut(s) 246, 503, 1179, 2353, 2772
Hsp92I GRCGYC 2 cut(s) 45, 69
HspAI GCGC 3 cut(s) 69, 2866, 2868
KasI GGCGCC 1 cut(s) 68
Kpn2I TCCGGA 3 cut(s) 38, 1889, 2269
KpnI GGTACC 1 cut(s) 1391
LguI GCTCTTC 1 cut(s) 899
LmnI GCTCC 3 cut(s) 302, 1472, 2362
Lsp1109I GCAGC 4 cut(s) 2396, 2741, 2926, 2929
LweI GCATC 7 cut(s) 79, 1235, 1342, 1483, 1909, 2710, 2831
MaeI CTAG 6 cut(s) 93, 522, 747, 1173, 1212, 2063
MaeII ACGT 5 cut(s) 246, 503, 1179, 2353, 2772
MbiI CCGCTC 2 cut(s) 210, 1059
MflI RGATCY 3 cut(s) 131, 1867, 2321
MlsI TGGCCA 1 cut(s) 472
MluNI TGGCCA 1 cut(s) 472
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 2 cut(s) 206, 2854
MmeI TCCRAC 4 cut(s) 45, 204, 409, 1669
Mox20I TGGCCA 1 cut(s) 472
Mph1103I ATGCAT 1 cut(s) 1498
MroI TCCGGA 3 cut(s) 38, 1889, 2269
MscI TGGCCA 1 cut(s) 472
MseI TTAA 9 cut(s) 819, 840, 921, 1038, 1319, 1655, 1767, 2414, 2736
MslI CAYNNNNRTG 2 cut(s) 2106, 2573
Msp20I TGGCCA 1 cut(s) 472
MspA1I CMGCKG 3 cut(s) 1724, 2489, 2911
MspR9I CCNGG 8 cut(s) 474, 584, 655, 756, 829, 1085, 2156, 2520
MvaI CCWGG 6 cut(s) 474, 655, 756, 829, 1085, 2520
MvnI CGCG 3 cut(s) 26, 2866, 2868
MwoI GCNNNNNNNGC 9 cut(s) 53, 123, 998, 1030, 1039, 2569, 2633, 2729, 2917
NarI GGCGCC 1 cut(s) 69
NciI CCSGG 2 cut(s) 584, 2156
NmeAIII GCCGAG 1 cut(s) 607
NmuCI GTSAC 8 cut(s) 882, 1535, 1679, 1781, 1882, 2187, 2219, 2975
NsiI ATGCAT 1 cut(s) 1498
NspI RCATGY 2 cut(s) 2429, 2694
NspV TTCGAA 1 cut(s) 15
PaeI GCATGC 1 cut(s) 2429
PaeR7I CTCGAG 2 cut(s) 1044, 2985
PauI GCGCGC 1 cut(s) 2866
PceI AGGCCT 2 cut(s) 494, 2006
PciI ACATGT 1 cut(s) 2690
PciSI GCTCTTC 1 cut(s) 899
PcsI WCGNNNNNNNCGW 3 cut(s) 30, 576, 1009
PfeI GAWTC 9 cut(s) 17, 35, 202, 724, 1694, 2015, 2483, 2876, 2956
PinAI ACCGGT 1 cut(s) 1427
Ple19I CGATCG 1 cut(s) 2368
PleI GAGTC 2 cut(s) 205, 2854
PluTI GGCGCC 1 cut(s) 72
PpsI GAGTC 2 cut(s) 205, 2854
PscI ACATGT 1 cut(s) 2690
Psp124BI GAGCTC 1 cut(s) 409
Psp6I CCWGG 6 cut(s) 472, 653, 754, 827, 1083, 2518
PspFI CCCAGC 2 cut(s) 2342, 2463
PspGI CCWGG 6 cut(s) 472, 653, 754, 827, 1083, 2518
PspOMI GGGCCC 1 cut(s) 2157
PspXI VCTCGAGB 2 cut(s) 1044, 2985
PsrI GAACNNNNNNTAC 2 cut(s) 2271, 2303
PstI CTGCAG 1 cut(s) 1482
PsuI RGATCY 3 cut(s) 131, 1867, 2321
PteI GCGCGC 1 cut(s) 2866
PvuI CGATCG 1 cut(s) 2368
PvuII CAGCTG 1 cut(s) 1724
RsaI GTAC 4 cut(s) 745, 761, 1389, 2711
RsaNI GTAC 4 cut(s) 744, 760, 1388, 2710
RseI CAYNNNNRTG 2 cut(s) 2106, 2573
SacI GAGCTC 1 cut(s) 409
SapI GCTCTTC 1 cut(s) 899
SaqAI TTAA 9 cut(s) 819, 840, 921, 1038, 1319, 1655, 1767, 2414, 2736
SbfI CCTGCAGG 1 cut(s) 1482
SchI GAGTC 2 cut(s) 206, 2854
ScrFI CCNGG 8 cut(s) 474, 584, 655, 756, 829, 1085, 2156, 2520
SdaI CCTGCAGG 1 cut(s) 1482
SfaNI GCATC 7 cut(s) 79, 1235, 1342, 1483, 1909, 2710, 2831
SfcI CTRYAG 1 cut(s) 1478
SfoI GGCGCC 1 cut(s) 70
Sfr274I CTCGAG 2 cut(s) 1044, 2985
SfuI TTCGAA 1 cut(s) 15
SinI GGWCC 7 cut(s) 1061, 1126, 1560, 1616, 2182, 2645, 2807
SlaI CTCGAG 2 cut(s) 1044, 2985
SmiMI CAYNNNNRTG 2 cut(s) 2106, 2573
SmlI CTYRAG 2 cut(s) 1044, 2985
SmoI CTYRAG 2 cut(s) 1044, 2985
SpeI ACTAGT 2 cut(s) 746, 2062
SphI GCATGC 1 cut(s) 2429
Sse8387I CCTGCAGG 1 cut(s) 1482
SseBI AGGCCT 2 cut(s) 494, 2006
SspDI GGCGCC 1 cut(s) 68
SspMI CTAG 6 cut(s) 93, 522, 747, 1173, 1212, 2063
SstI GAGCTC 1 cut(s) 409
StuI AGGCCT 2 cut(s) 494, 2006
StyD4I CCNGG 8 cut(s) 472, 582, 653, 754, 827, 1083, 2154, 2518
StyI CCWWGG 3 cut(s) 892, 1414, 2550
TaaI ACNGT 8 cut(s) 383, 419, 1293, 1687, 1842, 1996, 2807, 2936
TaiI ACGT 5 cut(s) 249, 506, 1182, 2356, 2775
TaqII GACCGA 1 cut(s) 993
TatI WGTACW 1 cut(s) 2709
TauI GCSGC 7 cut(s) 69, 126, 213, 216, 517, 1226, 2914
TfiI GAWTC 9 cut(s) 17, 35, 202, 724, 1694, 2015, 2483, 2876, 2956
Tru1I TTAA 9 cut(s) 819, 840, 921, 1038, 1319, 1655, 1767, 2414, 2736
Tru9I TTAA 9 cut(s) 819, 840, 921, 1038, 1319, 1655, 1767, 2414, 2736
TscAI CASTG 4 cut(s) 963, 1690, 1707, 2941
TseFI GTSAC 8 cut(s) 882, 1535, 1679, 1781, 1882, 2187, 2219, 2975
TseI GCWGC 4 cut(s) 2384, 2729, 2914, 2917
Tsp45I GTSAC 8 cut(s) 882, 1535, 1679, 1781, 1882, 2187, 2219, 2975
TspRI CASTG 4 cut(s) 963, 1690, 1707, 2941
VneI GTGCAC 1 cut(s) 3002
VpaK11BI GGWCC 7 cut(s) 1061, 1126, 1560, 1616, 2182, 2645, 2807
XagI CCTNNNNNAGG 1 cut(s) 2214
XapI RAATTY 3 cut(s) 646, 2259, 2582
XceI RCATGY 2 cut(s) 2429, 2694
XcmI CCANNNNNNNNNTGG 1 cut(s) 1804
XhoI CTCGAG 2 cut(s) 1044, 2985
XspI CTAG 6 cut(s) 93, 522, 747, 1173, 1212, 2063
Zsp2I ATGCAT 1 cut(s) 1498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.