Rw3G005230

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0010008
Physical Location & Seq
Forward (+)
4386425 .. 4390829
4405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G005230.1

Sequence Viewer

Length: 3606 bp
ATGAGCAACGATTTGGAATCGGATGCGAAGTTGGAATCCGCTGACGACGTTAAGGAAGCTGAACCTAAGGACGCCGGCGTTGGTAGCCCCGAGGATGAGGCTAGGGTTTCGCCAATGGAGCTCGATTTAGAGGCCGAGGGCGGCGGTTCTATGGGCTCCGAGGGCGGGAGATCTGATAGAGTTAGGGTTAGTCCGGAGAGTGGCGATGGAAATGTAGATAAGGGAATGGAATCGAAAGGGTTTAAAATTCAGGGCGGCGATGGATTGGGTAACGATGATATTGATGACGTGTTTGATATTGCGAGAGTTGAAATTGATGATCAGGATGAGGATAGCTCTGAGGCTCAGAATGAGAGTGTGGATTTGTTATCTGAGTTTGATGAGTATGTGGCTAATGAGAAGGACGGAATGGCTTTGGGAACCTCCAGAGCTCTGAGCTATGGTTTCGAAGTCGGTGATATGGTGTGGGGGAAGGTCAAGTCTCATCCCTGGTGGCCGGGGCATATATTTAATGAGGCCTTTGCCACGTCTCAGGTGCGCCGGACACGGAGGGAAGGTCATGTCCTGGTGGCTTTCTTTGGTGATAGTAGCTACGGCTGGTTCGACCCGGCTGAGCTTATCCCGTTCGAGCCCCATTTCGCTGAGAAGTCTCGGCAGACGAATTACAGGAATTTTAACAGGGCTGTAGAGGAGGCTGTTGATGAGGCGAGTCGCAGGTGTGGAGTTGGTTTCGTGTGTAAGTGTAGGAATCCGTATAATTTTCGGGCCACTAGTGTGCCGGGGTACTTTGTTGTTGATGTGCCGGATTATGAGCACGGAGCAGTGTACTCTACGGATCAGATTAGGAAGGCGAGGGATGGTTTTAATCCTGTTGAGATGGTGGCTCTCGTGAAGCAGTTGGCAAGCTCACCAGTCCAAGGTGATCAGAAGAGCCTTAGCTTCATTACGAATAAGGCTACTCTATTTGCTTATCGGAAGGCTGTGTTTGAAGAGTATGATGAGACCTATGCTCAGGCATTTGGGGCTCGGTCGTCTTCACGTAATGCAGTTGCAGTGTCAGATCAGCCTGTTAAGCCTCGAGCACCTTTGAGTGGTCCTTTGGTGATTGCTGAAGCTCTGGGTGGGCGGAAGAGTGCCACAAAATCTATGAAAGTCAAGGACCATTCAAAGAAAGACAAATACCTTTTCAAGCGGAGAGATGAAGCCAGCAATGTGAAATCCCACCAAAGTACACAGGGCCAAGCGAGTTCCTCAGCCCCATCTGCGTACTTGGAGGGATCAATAGCATTAGGAGATGGAGATTTTATGTTACAAAAGAGGGCTCCATCTCCCTCTATGAAACTGCAGATTTTAAAACATGAGCAGACTGAGTTTATGAGCAAGGATGCATCCGGTAAAGAAACTGTAAACTTAAATCAGGTACCAGCAAACAGCTCTCTTACCAGCCAAGGTGTTACCACTGGTGCAAAGCTATCTCTAAAGCTTTCTCTGGACAAGGAAAGTGGAGCCCTGCGGGAGGTGAAAGAGGTATTGACACAGAATGTAGCTGTAGGTCCTGCAAGCACAGGCTGCTCTGATTTATTGGGTGAAGGGACCAAGCAATGTATAAAAGATGAAACATCAGAGTCTTTGAAGCAGGAAGGGGAAGGTCCGATGGAGGTTAAAAATGAGGAGAGTGCCAAATTGTCTGGTTCATATGACAATTTACAACCACCTTTGAGTTTTCCAAAGAAAGTGGAGGAAGGCAATGAATTGAATGAAGTTCATATTGGTCACAGTGTTGGAGATTCTTCACCAATTGAGGCAAAGAGTTCAGCTGGAAAGAAGGCTGTTGGTGGAGTGAAAAAGGCCAAGGTTCTTAAACGGCCACTGGGTGACATGAACCCTGAGAACTCTATTATGGGAGACAAAAAGAAGAAGAAGAAGAAGAAACAGTTGGGTTCAGAAACAAGCTTCAGAGATCCACAGAGGACTTTGACTTCTGGAAAAGTGAGTTCCTTGGTGGGTAAGGTAGCTGGAAAGCCCAACCATGCAGGATTGGCTCCAGAAGAAGAGTTTCAGGTAGAACATCATAAGAAAGATGTTGCTGTTAATAATGAATTGTCTGGATCTGTTGGAACATTGCCGATTCTTAAAGAGCTTGAATTACCACAACTAGTAAGCGATTTGCAAGCCCTTGCACTTGATCCTTTTCATGGTCGTGAAACAAACAACCCTGCAATTGTTCGGCAGTTCTTTCTGCAATTCCGGGCCCTTGTCTATCAGAAAAGCTTGGTTCTATCACCGCCATCTGAAACAGAGCTTGTTGAAGGTCGTAGCTCTAAAACTCCATCTGGTCTCAAAGCCTCAGAAATTTCTCCTCGGGAACCAGTTAGAGATGTACCCTCCTCGAAGGCTGGAAAACCCTTGTTCAGATCCGATGACCGCACCATAGCTGGGCGGAAACGTGCTCCATCTGATCGTCAAGGAGAGATTGCAGCTAAGAAGTCCAAGAAAATGAGTGATTTAAAATCATTGGCTGCAGAAAGGAAGATTGGTCTAAAAAGTTCAGAGACCCAGCGTGGAGAGGTAAAAGAAACAGCTGTGCCCATATCGCGGAAGTCACTGAAGCCAGGTCTTGTGAAGAAAATGGAACCTCCATCCAAGGTTGTTGAGCCCACCATGCTTGTGATGAAATTCCCTCCCAAAATCTCTCTGCCTTCACCTGCAGAACTGAAGGCAAAATTTGCTCGTTTTGGACCAATGGATCAGTCTGGTCTCCGTGTGTTCTACAAGTCCTCGACATGCAGAGTTGTATTTCTGTACAAATCTGATGCACAAGCAGCATTGAAGGTTGCTACTTCAAACAACTCCTTTTTTGGCAATGTTAGTGTGAGGTTCCAGCTTCGAGAGGTGGACGGTCCTGAAGTTCCTGCATCTGGGAAGGGCAATGGAGATGACAATTCTACTGAGACCCCGCGGGCTAAGGATTCTGCATTCATGCCATCTCCGGCACTCAAACAGCGGCAGCAGCTGCAGTCTCTCTCACACAGTGCAATCCAGCCGAAATCGATTCTGAAGAAGTCATCGGGTGATGAACCACGAGGACAGGTAACTGGTGGCAATGGAAATAGTAAAGGAACTGCACGTGTAAAATTCATGTTGGGTGGGGAAGAACCTAGTAGAAATGAGCAATTGATGATGCCTGGTAATAGAAATAACAACTTCAACAACAACAGTGCTAGTTTTGCAGATGATGGTGCACCTTCTTCTTCTACTTCTGTTGCTATGATGAATTATAATGCTAGAAACTTTCAAAAGGTTACTCCTCCACCCTCTTCATTGCCTCCTATACTTCCGCTTCCTCATAGTCGGCCTCCACCTTCTCAATATGCAAAACCCCCACCACCACATAGTAATTTTTCACAGCAGCATACGGAAATGACGCCACCTCCACCACCACCACCAAGCAGAAGCAGTCACAATATTCTCAACACACCCTCCACATTTCCGAGTGCACCCACAGTTGATATTTCCCAGCAGATGCTAAGCCTGTTGACGAGGTGCAATGATGTTGTGACCAACGTACAGGGCTTGCTTGGATATGTGCCCTACCATCCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
PWWP

Protein Analysis

1201

Amino Acids

130.28

Weight (kDa)

8.48

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 152 - 236 1.1e-18 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3279
AarI CACCTGC 2 cut(s) 705, 2713
Acc36I ACCTGC 2 cut(s) 705, 2713
Acc65I GGTACC 1 cut(s) 1420
AccB1I GGYRCC 1 cut(s) 1420
AccII CGCG 2 cut(s) 2596, 2959
AccIII TCCGGA 1 cut(s) 193
AclWI GGATC 7 cut(s) 843, 1285, 1955, 2116, 2180, 2409, 2754
AcoI YGGCCR 2 cut(s) 494, 1865
AcsI RAATTY 6 cut(s) 246, 670, 2352, 2675, 2723, 3134
AcuI CTGAAG 6 cut(s) 1131, 1939, 2627, 2735, 2925, 3077
AcvI CACGTG 1 cut(s) 3128
AcyI GRCGYC 2 cut(s) 72, 3425
AdeI CACNNNGTG 2 cut(s) 1874, 3032
AfaI GTAC 8 cut(s) 785, 827, 1231, 1268, 1422, 2382, 2804, 3567
AfiI CCNNNNNNNGG 9 cut(s) 165, 200, 917, 1091, 1516, 2195, 2436, 2595, 2918
AflIII ACRYGT 2 cut(s) 288, 3127
AhlI ACTAGT 2 cut(s) 770, 2155
AjiI CACGTC 2 cut(s) 289, 528
AjnI CCWGG 4 cut(s) 488, 564, 2611, 3184
AjuI GAANNNNNNNTTGG 6 cut(s) 1752, 1784, 1919, 1951, 2517, 2549
AleI CACNNNNGTG 1 cut(s) 773
Alw21I GWGCWC 7 cut(s) 123, 433, 816, 1084, 2452, 3244, 3499
Alw44I GTGCAC 2 cut(s) 3240, 3495
AlwI GGATC 7 cut(s) 843, 1285, 1955, 2116, 2180, 2409, 2754
AlwNI CAGNNNCTG 1 cut(s) 3013
Ama87I CYCGRG 3 cut(s) 89, 1077, 2361
Aor13HI TCCGGA 1 cut(s) 193
AoxI GGCC 9 cut(s) 132, 494, 516, 765, 1237, 1848, 1865, 2250, 3353
ApaI GGGCCC 1 cut(s) 2254
ApaLI GTGCAC 2 cut(s) 3240, 3495
ApeKI GCWGC 8 cut(s) 1569, 2477, 2519, 2822, 3007, 3010, 3013, 3409
ApoI RAATTY 6 cut(s) 246, 670, 2352, 2675, 2723, 3134
Asp700I GAANNNNTTC 1 cut(s) 2055
Asp718I GGTACC 1 cut(s) 1420
AspLEI GCGC 1 cut(s) 540
AsuC2I CCSGG 4 cut(s) 498, 608, 780, 2249
AsuII TTCGAA 1 cut(s) 447
AvaI CYCGRG 3 cut(s) 89, 1077, 2361
AvaII GGWCC 7 cut(s) 1094, 1159, 1553, 1593, 1649, 2738, 2900
AxyI CCTNAGG 1 cut(s) 66
BaeGI GKGCMC 5 cut(s) 2254, 2589, 3244, 3499, 3591
BanI GGYRCC 1 cut(s) 1420
BanII GRGCYC 9 cut(s) 123, 158, 433, 633, 1027, 1324, 1510, 2254, 2658
BauI CACGAG 2 cut(s) 887, 3081
BbrPI CACGTG 1 cut(s) 3128
BbsI GAAGAC 1 cut(s) 1026
Bbv12I GWGCWC 7 cut(s) 123, 433, 816, 1084, 2452, 3244, 3499
BbvCI CCTCAGC 1 cut(s) 1252
BbvI GCAGC 8 cut(s) 1556, 2489, 2506, 2834, 3000, 3019, 3022, 3421
BceAI ACGGC 2 cut(s) 610, 1880
BciT130I CCWGG 4 cut(s) 490, 566, 2613, 3186
BclI TGATCA 2 cut(s) 319, 922
BcnI CCSGG 4 cut(s) 498, 608, 780, 2249
BcuI ACTAGT 2 cut(s) 770, 2155
BfaI CTAG 6 cut(s) 102, 771, 2156, 3159, 3222, 3285
BfmI CTRYAG 6 cut(s) 684, 1343, 1548, 2520, 2706, 3014
BfuAI ACCTGC 2 cut(s) 705, 2713
BglII AGATCT 1 cut(s) 170
BlpI GCTNAGC 2 cut(s) 612, 3527
Bme1390I CCNGG 8 cut(s) 490, 498, 566, 608, 780, 2249, 2613, 3186
Bme18I GGWCC 7 cut(s) 1094, 1159, 1553, 1593, 1649, 2738, 2900
BmeT110I CYCGRG 3 cut(s) 89, 1077, 2361
BmgBI CACGTC 2 cut(s) 289, 528
BmrFI CCNGG 8 cut(s) 490, 498, 566, 608, 780, 2249, 2613, 3186
BmrI ACTGGG 1 cut(s) 1880
BmsI GCATC 7 cut(s) 13, 1375, 1397, 2803, 2924, 3171, 3513
BmuI ACTGGG 1 cut(s) 1880
BpiI GAAGAC 1 cut(s) 1026
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpmI CTGGAG 2 cut(s) 409, 2028
Bpu10I CCTNAGC 4 cut(s) 935, 1011, 1252, 2964
Bpu1102I GCTNAGC 2 cut(s) 612, 3527
Bpu14I TTCGAA 1 cut(s) 447
BpuMI CCSGG 4 cut(s) 498, 608, 780, 2249
Bsa29I ATCGAT 1 cut(s) 3050
BsaAI YACGTR 2 cut(s) 1040, 3128
BsaHI GRCGYC 2 cut(s) 72, 3425
BsaI GGTCTC 5 cut(s) 995, 2342, 2546, 2762, 2945
BsaWI WCCGGW 2 cut(s) 193, 1391
BsaXI ACNNNNNCTCC 6 cut(s) 541, 571, 3415, 3445, 3464, 3494
Bsc4I CCNNNNNNNGG 9 cut(s) 165, 200, 917, 1091, 1516, 2195, 2436, 2595, 2918
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 5 cut(s) 911, 1465, 1875, 2369, 3100
Bse21I CCTNAGG 1 cut(s) 66
Bse3DI GCAATG 9 cut(s) 1216, 1607, 1753, 2120, 2869, 2935, 3109, 3320, 3553
BseAI TCCGGA 1 cut(s) 193
BseBI CCWGG 4 cut(s) 490, 566, 2613, 3186
BseCI ATCGAT 1 cut(s) 3050
BseGI GGATG 9 cut(s) 28, 100, 331, 484, 862, 1388, 1390, 2639, 3595
BseLI CCNNNNNNNGG 9 cut(s) 165, 200, 917, 1091, 1516, 2195, 2436, 2595, 2918
BseMI GCAATG 9 cut(s) 1216, 1607, 1753, 2120, 2869, 2935, 3109, 3320, 3553
BseNI ACTGG 5 cut(s) 911, 1465, 1875, 2369, 3100
BseRI GAGGAG 5 cut(s) 704, 1685, 2349, 2377, 3297
BseSI GKGCMC 5 cut(s) 2254, 2589, 3244, 3499, 3591
BseXI GCAGC 8 cut(s) 1556, 2489, 2506, 2834, 3000, 3019, 3022, 3421
BseYI CCCAGC 3 cut(s) 2435, 2556, 3516
BsgI GTGCAG 1 cut(s) 3108
Bsh1236I CGCG 2 cut(s) 2596, 2959
Bsh1285I CGRYCG 1 cut(s) 1031
BshFI GGCC 9 cut(s) 134, 496, 518, 767, 1239, 1850, 1867, 2252, 3355
BshNI GGYRCC 1 cut(s) 1420
BshVI ATCGAT 1 cut(s) 3050
BsiEI CGRYCG 1 cut(s) 1031
BsiHKAI GWGCWC 7 cut(s) 123, 433, 816, 1084, 2452, 3244, 3499
BsiHKCI CYCGRG 3 cut(s) 89, 1077, 2361
BslFI GGGAC 1 cut(s) 1606
BslI CCNNNNNNNGG 9 cut(s) 165, 200, 917, 1091, 1516, 2195, 2436, 2595, 2918
BsmBI CGTCTC 1 cut(s) 534
BsmFI GGGAC 1 cut(s) 1606
BsmI GAATGC 1 cut(s) 2975
BsnI GGCC 9 cut(s) 134, 496, 518, 767, 1239, 1850, 1867, 2252, 3355
Bso31I GGTCTC 5 cut(s) 995, 2342, 2546, 2762, 2945
BsoBI CYCGRG 3 cut(s) 89, 1077, 2361
Bsp119I TTCGAA 1 cut(s) 447
Bsp120I GGGCCC 1 cut(s) 2250
Bsp13I TCCGGA 1 cut(s) 193
Bsp1407I TGTACA 1 cut(s) 2802
Bsp1720I GCTNAGC 2 cut(s) 612, 3527
BspANI GGCC 9 cut(s) 134, 496, 518, 767, 1239, 1850, 1867, 2252, 3355
BspDI ATCGAT 1 cut(s) 3050
BspEI TCCGGA 1 cut(s) 193
BspFNI CGCG 2 cut(s) 2596, 2959
BspMAI CTGCAG 4 cut(s) 1347, 2524, 2710, 3018
BspMI ACCTGC 2 cut(s) 705, 2713
BspPI GGATC 7 cut(s) 843, 1285, 1955, 2116, 2180, 2409, 2754
BspQI GCTCTTC 1 cut(s) 923
BspT104I TTCGAA 1 cut(s) 447
BspT107I GGYRCC 1 cut(s) 1420
BspTNI GGTCTC 5 cut(s) 995, 2342, 2546, 2762, 2945
BsrDI GCAATG 9 cut(s) 1216, 1607, 1753, 2120, 2869, 2935, 3109, 3320, 3553
BsrFI RCCGGY 1 cut(s) 74
BsrGI TGTACA 1 cut(s) 2802
BsrI ACTGG 5 cut(s) 911, 1465, 1875, 2369, 3100
BssAI RCCGGY 1 cut(s) 74
BssNI GRCGYC 2 cut(s) 72, 3425
BssSI CACGAG 2 cut(s) 887, 3081
BssT1I CCWWGG 5 cut(s) 916, 1447, 1851, 1998, 2643
Bst2BI CACGAG 2 cut(s) 887, 3081
Bst2UI CCWGG 4 cut(s) 490, 566, 2613, 3186
Bst4CI ACNGT 7 cut(s) 1405, 1778, 1935, 2900, 3032, 3218, 3505
Bst6I CTCTTC 5 cut(s) 923, 984, 1124, 2046, 3322
BstACI GRCGYC 2 cut(s) 72, 3425
BstAPI GCANNNNNTGC 3 cut(s) 1569, 2726, 3013
BstAUI TGTACA 1 cut(s) 2802
BstBAI YACGTR 2 cut(s) 1040, 3128
BstBI TTCGAA 1 cut(s) 447
BstC8I GCNNGC 7 cut(s) 76, 904, 1207, 1561, 2172, 2961, 3575
BstDSI CCRYGG 1 cut(s) 2957
BstENI CCTNNNNNAGG 1 cut(s) 1514
BstF5I GGATG 9 cut(s) 28, 100, 331, 484, 862, 1388, 1390, 2639, 3595
BstFNI CGCG 2 cut(s) 2596, 2959
BstHHI GCGC 1 cut(s) 540
BstMCI CGRYCG 1 cut(s) 1031
BstNI CCWGG 4 cut(s) 490, 566, 2613, 3186
BstNSI RCATGY 1 cut(s) 2787
BstSCI CCNGG 8 cut(s) 488, 496, 564, 606, 778, 2247, 2611, 3184
BstSFI CTRYAG 6 cut(s) 684, 1343, 1548, 2520, 2706, 3014
BstSLI GKGCMC 5 cut(s) 2254, 2589, 3244, 3499, 3591
BstUI CGCG 2 cut(s) 2596, 2959
BstV1I GCAGC 8 cut(s) 1556, 2489, 2506, 2834, 3000, 3019, 3022, 3421
BstV2I GAAGAC 1 cut(s) 1026
BstX2I RGATCY 4 cut(s) 170, 1960, 2108, 2414
BstYI RGATCY 4 cut(s) 170, 1960, 2108, 2414
Bsu15I ATCGAT 1 cut(s) 3050
Bsu36I CCTNAGG 1 cut(s) 66
BsuRI GGCC 9 cut(s) 134, 496, 518, 767, 1239, 1850, 1867, 2252, 3355
BsuTUI ATCGAT 1 cut(s) 3050
BtgI CCRYGG 1 cut(s) 2957
BtgZI GCGATG 2 cut(s) 219, 273
BtrI CACGTC 2 cut(s) 289, 528
BtsCI GGATG 9 cut(s) 28, 100, 331, 484, 862, 1388, 1390, 2639, 3595
BtsI GCAGTG 2 cut(s) 828, 1059
BtsIMutI CAGTG 8 cut(s) 828, 1059, 1458, 1783, 1868, 2603, 3037, 3223
BveI ACCTGC 2 cut(s) 705, 2713
Cac8I GCNNGC 7 cut(s) 76, 904, 1207, 1561, 2172, 2961, 3575
CaiI CAGNNNCTG 1 cut(s) 3013
CfoI GCGC 1 cut(s) 540
Cfr10I RCCGGY 1 cut(s) 74
Cfr42I CCGCGG 1 cut(s) 2960
ClaI ATCGAT 1 cut(s) 3050
CseI GACGC 2 cut(s) 80, 3433
Csp6I GTAC 8 cut(s) 784, 826, 1230, 1267, 1421, 2381, 2803, 3566
CspCI CAANNNNNGTGG 6 cut(s) 1483, 1518, 1716, 1751, 2647, 2682
CviAII CATG 9 cut(s) 560, 1358, 1879, 2030, 2195, 2662, 2784, 2980, 3139
CviQI GTAC 8 cut(s) 784, 826, 1230, 1267, 1421, 2381, 2803, 3566
DraI TTTAAA 3 cut(s) 244, 1353, 2508
DraIII CACNNNGTG 2 cut(s) 1874, 3032
EaeI YGGCCR 2 cut(s) 494, 1865
Eam1104I CTCTTC 5 cut(s) 923, 984, 1124, 2046, 3322
EarI CTCTTC 5 cut(s) 923, 984, 1124, 2046, 3322
EciI GGCGGA 2 cut(s) 1141, 2455
Ecl136II GAGCTC 2 cut(s) 121, 431
Eco130I CCWWGG 5 cut(s) 916, 1447, 1851, 1998, 2643
Eco147I AGGCCT 1 cut(s) 518
Eco24I GRGCYC 9 cut(s) 123, 158, 433, 633, 1027, 1324, 1510, 2254, 2658
Eco31I GGTCTC 5 cut(s) 995, 2342, 2546, 2762, 2945
Eco47I GGWCC 7 cut(s) 1094, 1159, 1553, 1593, 1649, 2738, 2900
Eco53kI GAGCTC 2 cut(s) 121, 431
Eco57I CTGAAG 6 cut(s) 1131, 1939, 2627, 2735, 2925, 3077
Eco72I CACGTG 1 cut(s) 3128
Eco81I CCTNAGG 1 cut(s) 66
Eco88I CYCGRG 3 cut(s) 89, 1077, 2361
EcoICRI GAGCTC 2 cut(s) 121, 431
EcoNI CCTNNNNNAGG 1 cut(s) 1514
EcoO109I RGGNCCY 2 cut(s) 1553, 2251
EcoRII CCWGG 4 cut(s) 488, 564, 2611, 3184
EcoT14I CCWWGG 5 cut(s) 916, 1447, 1851, 1998, 2643
EcoT22I ATGCAT 1 cut(s) 1390
EcoT38I GRGCYC 9 cut(s) 123, 158, 433, 633, 1027, 1324, 1510, 2254, 2658
ErhI CCWWGG 5 cut(s) 916, 1447, 1851, 1998, 2643
Esp3I CGTCTC 1 cut(s) 534
FaeI CATG 9 cut(s) 563, 1361, 1882, 2033, 2198, 2665, 2787, 2983, 3142
FaqI GGGAC 1 cut(s) 1606
FatI CATG 9 cut(s) 559, 1357, 1878, 2029, 2194, 2661, 2783, 2979, 3138
FauI CCCGC 4 cut(s) 158, 1506, 2952, 2964
FauNDI CATATG 1 cut(s) 1696
FbaI TGATCA 2 cut(s) 319, 922
FokI GGATG 9 cut(s) 35, 107, 338, 471, 869, 1375, 1397, 2626, 3582
FriOI GRGCYC 9 cut(s) 123, 158, 433, 633, 1027, 1324, 1510, 2254, 2658
FspBI CTAG 6 cut(s) 102, 771, 2156, 3159, 3222, 3285
GlaI GCGC 1 cut(s) 539
GsaI CCCAGC 3 cut(s) 2439, 2560, 3520
GsuI CTGGAG 2 cut(s) 409, 2028
HaeIII GGCC 9 cut(s) 134, 496, 518, 767, 1239, 1850, 1867, 2252, 3355
HgaI GACGC 2 cut(s) 80, 3433
HhaI GCGC 1 cut(s) 540
Hin1I GRCGYC 2 cut(s) 72, 3425
Hin1II CATG 9 cut(s) 563, 1361, 1882, 2033, 2198, 2665, 2787, 2983, 3142
Hin6I GCGC 1 cut(s) 538
HinP1I GCGC 1 cut(s) 538
HincII GTYRAC 1 cut(s) 3537
HindII GTYRAC 1 cut(s) 3537
HindIII AAGCTT 3 cut(s) 1481, 1951, 2269
Hpy166II GTNNAC 7 cut(s) 826, 1232, 1408, 2896, 3242, 3497, 3537
Hpy8I GTNNAC 7 cut(s) 826, 1232, 1408, 2896, 3242, 3497, 3537
Hpy99I CGWCG 1 cut(s) 50
HpyCH4III ACNGT 7 cut(s) 1405, 1778, 1935, 2900, 3032, 3218, 3505
HpyCH4IV ACGT 7 cut(s) 48, 288, 527, 1039, 2446, 3127, 3564
HpySE526I ACGT 7 cut(s) 48, 288, 527, 1039, 2446, 3127, 3564
Hsp92I GRCGYC 2 cut(s) 72, 3425
Hsp92II CATG 9 cut(s) 563, 1361, 1882, 2033, 2198, 2665, 2787, 2983, 3142
HspAI GCGC 1 cut(s) 538
Kpn2I TCCGGA 1 cut(s) 193
KpnI GGTACC 1 cut(s) 1424
KroI GCCGGC 1 cut(s) 74
KroNI GCCGGC 1 cut(s) 76
Ksp22I TGATCA 2 cut(s) 319, 922
KspI CCGCGG 1 cut(s) 2960
LguI GCTCTTC 1 cut(s) 923
LmnI GCTCC 7 cut(s) 118, 161, 818, 1327, 1505, 2047, 2455
Lsp1109I GCAGC 8 cut(s) 1556, 2489, 2506, 2834, 3000, 3019, 3022, 3421
LweI GCATC 7 cut(s) 13, 1375, 1397, 2803, 2924, 3171, 3513
MaeI CTAG 6 cut(s) 102, 771, 2156, 3159, 3222, 3285
MaeII ACGT 7 cut(s) 48, 288, 527, 1039, 2446, 3127, 3564
MfeI CAATTG 3 cut(s) 1797, 2220, 3173
MflI RGATCY 4 cut(s) 170, 1960, 2108, 2414
MlyI GAGTC 2 cut(s) 718, 1634
MmeI TCCRAC 3 cut(s) 12, 1762, 2095
Mph1103I ATGCAT 1 cut(s) 1390
MreI CGCCGGCG 1 cut(s) 74
MroI TCCGGA 1 cut(s) 193
MroNI GCCGGC 1 cut(s) 74
MroXI GAANNNNTTC 1 cut(s) 2055
MslI CAYNNNNRTG 3 cut(s) 773, 2199, 2666
MspA1I CMGCKG 6 cut(s) 41, 1817, 2582, 2959, 3004, 3013
MspR9I CCNGG 8 cut(s) 490, 498, 566, 608, 780, 2249, 2613, 3186
MunI CAATTG 3 cut(s) 1797, 2220, 3173
Mva1269I GAATGC 1 cut(s) 2975
MvaI CCWGG 4 cut(s) 490, 566, 2613, 3186
MvnI CGCG 2 cut(s) 2596, 2959
NaeI GCCGGC 1 cut(s) 76
NciI CCSGG 4 cut(s) 498, 608, 780, 2249
NdeI CATATG 1 cut(s) 1696
NgoMIV GCCGGC 1 cut(s) 74
NlaIII CATG 9 cut(s) 563, 1361, 1882, 2033, 2198, 2665, 2787, 2983, 3142
NmeAIII GCCGAG 2 cut(s) 160, 631
NmuCI GTSAC 5 cut(s) 1772, 1874, 2601, 3458, 3556
NsiI ATGCAT 1 cut(s) 1390
NspI RCATGY 1 cut(s) 2787
NspV TTCGAA 1 cut(s) 447
OliI CACNNNNGTG 1 cut(s) 773
PaeR7I CTCGAG 1 cut(s) 1077
PaqCI CACCTGC 2 cut(s) 705, 2713
PceI AGGCCT 1 cut(s) 518
PciSI GCTCTTC 1 cut(s) 923
PcsI WCGNNNNNNNCGW 1 cut(s) 600
PctI GAATGC 1 cut(s) 2975
PdiI GCCGGC 1 cut(s) 76
PdmI GAANNNNTTC 1 cut(s) 2055
PfeI GAWTC 8 cut(s) 17, 35, 230, 748, 1787, 2128, 2969, 3052
PleI GAGTC 2 cut(s) 717, 1633
PmaCI CACGTG 1 cut(s) 3128
PmlI CACGTG 1 cut(s) 3128
PpsI GAGTC 2 cut(s) 717, 1633
Ppu21I YACGTR 2 cut(s) 1040, 3128
PpuMI RGGWCCY 1 cut(s) 1553
PsiI TTATAA 1 cut(s) 3279
Psp124BI GAGCTC 2 cut(s) 123, 433
Psp5II RGGWCCY 1 cut(s) 1553
Psp6I CCWGG 4 cut(s) 488, 564, 2611, 3184
PspCI CACGTG 1 cut(s) 3128
PspFI CCCAGC 3 cut(s) 2435, 2556, 3516
PspGI CCWGG 4 cut(s) 488, 564, 2611, 3184
PspOMI GGGCCC 1 cut(s) 2250
PspPPI RGGWCCY 1 cut(s) 1553
PspXI VCTCGAGB 1 cut(s) 1077
PstI CTGCAG 4 cut(s) 1347, 2524, 2710, 3018
PstNI CAGNNNCTG 1 cut(s) 3013
PsuI RGATCY 4 cut(s) 170, 1960, 2108, 2414
PvuII CAGCTG 3 cut(s) 1817, 2582, 3013
RsaI GTAC 8 cut(s) 785, 827, 1231, 1268, 1422, 2382, 2804, 3567
RsaNI GTAC 8 cut(s) 784, 826, 1230, 1267, 1421, 2381, 2803, 3566
RseI CAYNNNNRTG 3 cut(s) 773, 2199, 2666
SacI GAGCTC 2 cut(s) 123, 433
SacII CCGCGG 1 cut(s) 2960
SapI GCTCTTC 1 cut(s) 923
SchI GAGTC 2 cut(s) 718, 1634
ScrFI CCNGG 8 cut(s) 490, 498, 566, 608, 780, 2249, 2613, 3186
SfaNI GCATC 7 cut(s) 13, 1375, 1397, 2803, 2924, 3171, 3513
SfcI CTRYAG 6 cut(s) 684, 1343, 1548, 2520, 2706, 3014
Sfr274I CTCGAG 1 cut(s) 1077
Sfr303I CCGCGG 1 cut(s) 2960
SfuI TTCGAA 1 cut(s) 447
SgrAI CRCCGGYG 1 cut(s) 74
SgrBI CCGCGG 1 cut(s) 2960
SinI GGWCC 7 cut(s) 1094, 1159, 1553, 1593, 1649, 2738, 2900
SlaI CTCGAG 1 cut(s) 1077
SmiMI CAYNNNNRTG 3 cut(s) 773, 2199, 2666
SmlI CTYRAG 1 cut(s) 1077
SmoI CTYRAG 1 cut(s) 1077
SpeI ACTAGT 2 cut(s) 770, 2155
SseBI AGGCCT 1 cut(s) 518
SspI AATATT 1 cut(s) 3466
SspMI CTAG 6 cut(s) 102, 771, 2156, 3159, 3222, 3285
SstI GAGCTC 2 cut(s) 123, 433
StuI AGGCCT 1 cut(s) 518
StyD4I CCNGG 8 cut(s) 488, 496, 564, 606, 778, 2247, 2611, 3184
StyI CCWWGG 5 cut(s) 916, 1447, 1851, 1998, 2643
TaaI ACNGT 7 cut(s) 1405, 1778, 1935, 2900, 3032, 3218, 3505
TaiI ACGT 7 cut(s) 51, 291, 530, 1042, 2449, 3130, 3567
TaqII GACCGA 1 cut(s) 1017
TatI WGTACW 3 cut(s) 825, 1229, 2802
TauI GCSGC 3 cut(s) 144, 258, 3007
TfiI GAWTC 8 cut(s) 17, 35, 230, 748, 1787, 2128, 2969, 3052
TscAI CASTG 8 cut(s) 828, 1059, 1465, 1783, 1875, 2610, 3037, 3223
TseFI GTSAC 5 cut(s) 1772, 1874, 2601, 3458, 3556
TseI GCWGC 8 cut(s) 1569, 2477, 2519, 2822, 3007, 3010, 3013, 3409
Tsp45I GTSAC 5 cut(s) 1772, 1874, 2601, 3458, 3556
TspGWI ACGGA 7 cut(s) 420, 562, 741, 831, 848, 2750, 3431
TspRI CASTG 8 cut(s) 828, 1059, 1465, 1783, 1875, 2610, 3037, 3223
VneI GTGCAC 2 cut(s) 3240, 3495
VpaK11BI GGWCC 7 cut(s) 1094, 1159, 1553, 1593, 1649, 2738, 2900
XagI CCTNNNNNAGG 1 cut(s) 1514
XapI RAATTY 6 cut(s) 246, 670, 2352, 2675, 2723, 3134
XceI RCATGY 1 cut(s) 2787
XhoI CTCGAG 1 cut(s) 1077
XmnI GAANNNNTTC 1 cut(s) 2055
XspI CTAG 6 cut(s) 102, 771, 2156, 3159, 3222, 3285
Zsp2I ATGCAT 1 cut(s) 1390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.