MD12G1216400.v1.1

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010008
Physical Location & Seq
Reverse (-)
29406009 .. 29410779
4771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1216400.v1.1.491

Sequence Viewer

Length: 3501 bp
ATGAACAGCGATTTCGGATTGGACGGGAAATCGGATGCCACTGGAGAGGCCGAGGAAGCTAGAGCTAGGGTTTCCGAAGGCGGCGTCGGTGAAGATGGAGCTAGGGTTTCGCCAATGGAGCTCGATTCGGGGAGTGAGACTGGCGGAAGATCTGAGGAGGATAGGGCTAGGGTTAGTACGGAGAGTGAAGTTGGAAACGTAGATAAAGTGATGGAATCGAAGGGTTCTGGAATTCAGGTCGACAGAACTAGGGTGGAGGTTTCATTCAACGATGATGAAGATGATGTTTTCGACATTTCGCGGGTCGAAATTGATGACGATTCGGATAAATTTGAGGCTCACAGTGGTGAGCACCCGTCTTTGTTATCTGAGTTTGATGAGTTCGTGGCTAATGAGAAGAGTGGGATGGCGCTCGGAACCACGAGGGCGCTCAGCTACGGGTTCGAAGTGGGTGATATGGTGTGGGGGAAGGTAAAATCTCATCCGTGGTGGCCGGGGCACATATTTAACGAGGCGTTAGCAACGTCTCAGGTGCGGCGGACACGGAGGGAAGGTTATGTTTTGGTTGCCTTCTTTGGTGATAATAGTTACGGGTGGTTCGACCCGGCTGAACTCATACCATTTGATCCCCATTATGCTGAGAAATCTCGGCAGACAAGTCACAGGGGTTTTCTGAAGGCTGTGGAGGAGGCTGTTGATGAGGCGAATCGTAGGTGTGGAGTTGGTCTGGCTTGTAAATGTAGGAATGCCTATAATTTTCGGAGCACAAGTGTGCAAGGGTACTTTGTTGTTGATGTGCCGGATTATGAGCCAGGAGCAGTGTATTCGGAGAATCAGATTAGGAAGGCGAGGGATAGTTTTAAGCCGATTGAGATCCTGTCTTTTGTAAAGCAGTTGGCTTTGTCACCACTCGGTGATGATCAGAAGAGCCTCAGCTTTAATAAGAGCAAGGCTACTGCTTTTTCGTTTCGCAAGGCTGTTTTTGAAGAGTATGATGAGACGTACGCTCAGGCATTTGGTGCGCATCCAGGACGCCCTTCTCGTACTCTAGCTCCAGCTCCAACACGAGCTGCTTTGAGTGGTCCCTTGGTGATTGCTGAAGTTCTGGGTGGGCGGAAGACTGCCACAAAACCGATGAAAGTCAAGGACCATTCAAAGAGAGACAAATACCTATTTAAGCGGAGAGATGAACCTGTGAACTCAAAAATCCACCAAACTAGCCAGGGCCAAGCTAGTTCCTCAGCCCCATCTGCTGTCTTGGAGGGACCAATAGCGTTAGCAGATGATAATTATATGTTACAAAAGAGGGTTCCAGATGTATCTGGAAAAGAAGCTGTAATCATTGATCAGGCACCAAATAGCTCTCTCACCACCCGAGATGTTACTGTTGATGCAAAGCCATCTCTTGCAAAGGGAAGGGGGGATCTGCAGGAAGTAAAAGAGGGAGATGGTGATGTAGGTCCTACAACTACAGGATATGTTGATTTATTGGGGGAGGGGACCAAGCAACGTACAGTAGATGGTACATCACAACCGTTAAAGCAGGAAGGTGAGGGTGTGTTTGAGGTTAAATACGAAGAGAGTGAAAAATTGTCTGGATCATTTGAAAATCTTCAACAACCTTCTAATTCTTTGAAAAAAGAGGAAGGAGGCTATGAACGTGGTGTTGGAGATCCTTTGCCAATTGAGGCAAAGAGTTTAGGTGGGAAGAAAGCTGCTGGTGGAGTGAAAAAACTGAAGGTTCTTAAACGGCCTGCAGAGGACTTGGACACCAAGGACTCTATGATGGGGGATAAAAAGAAGAAGAAAAAGAAACAGTTAAGTGCAGAAGCAAGCGTTAGAAATCAGCAGAAGCCTTTGACTTCTGGAAAGGTACTGCCCTCAGGGAGTAAAGAAGCAGGAAATGCCAACCATGTAGGTTTGGCTCCAAGAGAGGATACCCCAGTTGAACATAAGAAAGATGTCACTTCCAGTAGTAACTTGTCTGAATCTGTTGGAAAATTGCCAATTCATGTCTTGGAAAATGTGCAGCTTGAACTATCACAACTGGTAAGTGATTTGAAGGCCCTTGCCCTTGATCCTTTTCACGGTATTGAGACAAACAGCCCTACAATTGTTCGGCAGTTCTTTCTGCACTTCCGGTCCCTTGTCTACCAGAAAAGTTTGGTTCTGTCACCACCCTCAGAGACTGAACCTGTTGAAGGCCGCTCCTCTAAATCTTCATCTGGTGTCAAAGCCTCTGATATATCTCCTACTGAGCAAGTTAGAGATTTATCGTCCTCAAAGCCAGCAAAACCCATGTTCAGATCCGATGATCCTACCATTGCTGGGCGGAAACGTGCTCCATCTGACCGTCAAGGAGATATTGCAGCTAAGAGGAACAAGAAAATTACTGATATAAGAACATTGGCTGCAGAAAAGAAGGCCATTCAGAGACCTCCTATAGACTCCAAGCGTGTAGAGGCAAAGGAATCAGCAACACCGGTTATGCGGAAGTCACTCAAGCCAGGTTTTGCAAAGAAAGCGGACCTTGCATCAAAGGCAGTTGAGCCCACAATGCTGGTGTTGAAGTTCCCTCCCAAAATCTCTCTCCCATCACCTGCAGAACTGAAGGCAAAGTTTGCTCGATTTGGGCCTATGGATCAGTCTGGTCTCCGTGTCTTTTGGAAGTCCTCGACATGCCGGGTTGTCTTTCTGTACAAATCTGATGCACATGCAGCATTTAAGTTTGCTACCGCAAACAGCTCCCTTTTCGGCAACTTCAGTGTGAGGTGTCAAATTCGAGAAGTGGGTGAAGTTCCAGAGTTCGACAAGGGTGACAATCCCAGGGACACCCCACGGGCTAAGGATCCATTGGTTGGGCAATCACCAGCATTGGCATCGGCACTCAGACAGCAGCAGCGGCAGGCTCAACAGAGTGCAGTGCAGCTGAAGTCGATTCTGAAGAAGTCGTCGGGCGAGGAGTCGGCAGGAGGGCAGGTAACCGGTGGGAATGGAAATAGTAAAGGGACTGCACGTGTAAAATTCATGTTGGGTAGGGAAGAAACTAGTAGGACTACTACTTCTGATCAATTCATGATGCCTGGTAATAGAAATAACAACTTCAACAAGAACAACAACAGCGCTAGTTTTGCGGACGTCGACGGTGCACCGTCTTCTACTTCTTCCATTGCTATGGATTTTAATACCTCTAGGAACTTTCAAAAGGTCAATCCTCCTCCAACTTTTTCATCACCATTGCCTCCTCTCCTCCCAAATCCTCCTGGTCCACCTCCGCAATATGCAAACCCCCCACCCCATAGTAATTTTCCGCAGCATTCCGAAATGGCACCACCCAGAAATACTAGTCACAATCTTAACGCACCACCCACCGCATTTCCAAGCACACCCACCAGCACCGTCGATATTTCGCAGCAGATGCTTAGCTTGTTGATAAGTTGCAGCGACGTTGTATCCAACGTGCAGGGCTTGCTTGGCTATTTGCCGTACCATCCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
PWWP

Protein Analysis

1167

Amino Acids

126.74

Weight (kDa)

8.6

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 151 - 235 7.1e-19 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2608
AatII GACGTC 1 cut(s) 3141
Acc16I TGCGCA 1 cut(s) 1023
Acc36I ACCTGC 2 cut(s) 2608, 2968
AccB1I GGYRCC 2 cut(s) 1351, 3328
AccB7I CCANNNNNTGG 1 cut(s) 2858
AccBSI CCGCTC 1 cut(s) 2208
AccI GTMKAC 3 cut(s) 240, 2151, 3141
AccII CGCG 1 cut(s) 301
AcoI YGGCCR 1 cut(s) 491
AcsI RAATTY 4 cut(s) 231, 329, 2778, 3023
AcuI CTGAAG 7 cut(s) 695, 1119, 1757, 2630, 2746, 2951, 2963
AcvI CACGTG 1 cut(s) 3017
AcyI GRCGYC 3 cut(s) 84, 1033, 3138
AdeI CACNNNGTG 1 cut(s) 914
AfaI GTAC 9 cut(s) 178, 782, 1004, 1045, 1513, 1525, 1875, 2699, 3488
AfeI AGCGCT 1 cut(s) 3124
AfiI CCNNNNNNNGG 4 cut(s) 2087, 2201, 2328, 2858
AflIII ACRYGT 1 cut(s) 3016
AgeI ACCGGT 2 cut(s) 2482, 2984
AhlI ACTAGT 2 cut(s) 3047, 3344
AjnI CCWGG 7 cut(s) 811, 1027, 1221, 2506, 2825, 3082, 3262
AjuI GAANNNNNNNTTGG 2 cut(s) 1650, 1682
AleI CACNNNNGTG 2 cut(s) 345, 770
Alw21I GWGCWC 5 cut(s) 123, 354, 767, 2344, 3151
Alw26I GTCTC 8 cut(s) 131, 531, 992, 1155, 2090, 2180, 2428, 2657
Alw44I GTGCAC 1 cut(s) 3147
AlwNI CAGNNNCTG 1 cut(s) 2189
Ama87I CYCGRG 1 cut(s) 1374
Aor51HI AGCGCT 1 cut(s) 3124
AoxI GGCC 8 cut(s) 48, 491, 1225, 1751, 2064, 2203, 2424, 2633
ApaLI GTGCAC 1 cut(s) 3147
ApoI RAATTY 4 cut(s) 231, 329, 2778, 3023
ArsI GACNNNNNNTTYG 2 cut(s) 69, 101
AsiGI ACCGGT 2 cut(s) 2482, 2984
Asp700I GAANNNNTTC 1 cut(s) 1611
AspLEI GCGC 4 cut(s) 412, 430, 1024, 3125
AsuC2I CCSGG 3 cut(s) 495, 605, 2684
AsuII TTCGAA 1 cut(s) 444
AvaI CYCGRG 1 cut(s) 1374
AvaII GGWCC 8 cut(s) 1082, 1147, 1265, 1460, 1500, 2142, 2527, 3266
AxyI CCTNAGG 1 cut(s) 1882
BaeGI GKGCMC 2 cut(s) 501, 3151
BaeI ACNNNNGTAYC 2 cut(s) 1929, 1962
BamHI GGATCC 1 cut(s) 2848
BanI GGYRCC 2 cut(s) 1351, 3328
BanII GRGCYC 2 cut(s) 123, 2553
BauI CACGAG 2 cut(s) 421, 1065
BbrPI CACGTG 1 cut(s) 3017
BbsI GAAGAC 2 cut(s) 1124, 3147
Bbv12I GWGCWC 5 cut(s) 123, 354, 767, 2344, 3151
BbvCI CCTCAGC 2 cut(s) 932, 1240
BceAI ACGGC 2 cut(s) 1766, 3469
BcgI CGANNNNNNTGC 4 cut(s) 807, 841, 2862, 2896
BciT130I CCWGG 7 cut(s) 813, 1029, 1223, 2508, 2827, 3084, 3264
BciVI GTATCC 2 cut(s) 1930, 3463
BclI TGATCA 3 cut(s) 919, 1345, 3067
BcnI CCSGG 3 cut(s) 495, 605, 2684
BcoDI GTCTC 8 cut(s) 131, 531, 992, 1155, 2090, 2180, 2428, 2657
BcuI ACTAGT 2 cut(s) 3047, 3344
BfmI CTRYAG 6 cut(s) 1427, 1470, 1755, 2412, 2442, 2601
BfoI RGCGCY 3 cut(s) 413, 431, 3126
BfuAI ACCTGC 2 cut(s) 2608, 2968
BfuI GTATCC 2 cut(s) 1930, 3463
BglII AGATCT 1 cut(s) 149
BlpI GCTNAGC 2 cut(s) 431, 3422
Bme18I GGWCC 8 cut(s) 1082, 1147, 1265, 1460, 1500, 2142, 2527, 3266
BmeT110I CYCGRG 1 cut(s) 1374
BmrI ACTGGG 1 cut(s) 1937
BmsI GCATC 8 cut(s) 25, 1033, 1381, 2543, 2698, 2888, 3069, 3408
BmuI ACTGGG 1 cut(s) 1937
BpiI GAAGAC 2 cut(s) 1124, 3147
BpmI CTGGAG 2 cut(s) 63, 1038
Bpu10I CCTNAGC 4 cut(s) 932, 1008, 1240, 2844
Bpu1102I GCTNAGC 2 cut(s) 431, 3422
Bpu14I TTCGAA 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 2486
BpuMI CCSGG 3 cut(s) 495, 605, 2684
BsaAI YACGTR 1 cut(s) 3017
BsaBI GATNNNNATC 1 cut(s) 872
BsaHI GRCGYC 3 cut(s) 84, 1033, 3138
BsaI GGTCTC 2 cut(s) 2428, 2657
BsaJI CCNNGG 9 cut(s) 51, 485, 494, 1086, 1222, 1773, 2825, 2826, 2837
BsaWI WCCGGW 3 cut(s) 2139, 2482, 2984
Bsc4I CCNNNNNNNGG 4 cut(s) 2087, 2201, 2328, 2858
Bse118I RCCGGY 2 cut(s) 2482, 2984
Bse1I ACTGG 5 cut(s) 46, 145, 1943, 1971, 2052
Bse21I CCTNAGG 1 cut(s) 1882
Bse3DI GCAATG 3 cut(s) 2322, 3168, 3236
Bse8I GATNNNNATC 1 cut(s) 872
BseBI CCWGG 7 cut(s) 813, 1029, 1223, 2508, 2827, 3084, 3264
BseDI CCNNGG 9 cut(s) 51, 485, 494, 1086, 1222, 1773, 2825, 2826, 2837
BseGI GGATG 5 cut(s) 40, 411, 481, 1024, 3490
BseJI GATNNNNATC 1 cut(s) 872
BseLI CCNNNNNNNGG 4 cut(s) 2087, 2201, 2328, 2858
BseMI GCAATG 3 cut(s) 2322, 3168, 3236
BseNI ACTGG 5 cut(s) 46, 145, 1943, 1971, 2052
BseRI GAGGAG 7 cut(s) 170, 701, 2200, 2975, 3207, 3234, 3239
BseSI GKGCMC 2 cut(s) 501, 3151
BseYI CCCAGC 1 cut(s) 2327
BsgI GTGCAG 7 cut(s) 1845, 2048, 2117, 2940, 2945, 2997, 3482
Bsh1236I CGCG 1 cut(s) 301
BshFI GGCC 8 cut(s) 50, 493, 1227, 1753, 2066, 2205, 2426, 2635
BshNI GGYRCC 2 cut(s) 1351, 3328
BshTI ACCGGT 2 cut(s) 2482, 2984
BsiHKAI GWGCWC 5 cut(s) 123, 354, 767, 2344, 3151
BsiHKCI CYCGRG 1 cut(s) 1374
BsiSI CCGG 7 cut(s) 494, 605, 800, 2140, 2483, 2683, 2985
BsiWI CGTACG 1 cut(s) 1002
BslFI GGGAC 6 cut(s) 1068, 1278, 1513, 2128, 2843, 3022
BslI CCNNNNNNNGG 4 cut(s) 2087, 2201, 2328, 2858
BsmAI GTCTC 8 cut(s) 131, 531, 992, 1155, 2090, 2180, 2428, 2657
BsmBI CGTCTC 2 cut(s) 531, 992
BsmFI GGGAC 6 cut(s) 1068, 1278, 1513, 2128, 2843, 3022
BsmI GAATGC 2 cut(s) 751, 3316
BsnI GGCC 8 cut(s) 50, 493, 1227, 1753, 2066, 2205, 2426, 2635
Bso31I GGTCTC 2 cut(s) 2428, 2657
BsoBI CYCGRG 1 cut(s) 1374
Bsp119I TTCGAA 1 cut(s) 444
Bsp1286I GDGCHC 7 cut(s) 123, 354, 501, 767, 2344, 2553, 3151
Bsp1407I TGTACA 1 cut(s) 2697
Bsp1720I GCTNAGC 2 cut(s) 431, 3422
BspANI GGCC 8 cut(s) 50, 493, 1227, 1753, 2066, 2205, 2426, 2635
BspFNI CGCG 1 cut(s) 301
BspHI TCATGA 1 cut(s) 3075
BspMAI CTGCAG 4 cut(s) 1431, 1759, 2416, 2605
BspMI ACCTGC 2 cut(s) 2608, 2968
BspQI GCTCTTC 1 cut(s) 920
BspT104I TTCGAA 1 cut(s) 444
BspT107I GGYRCC 2 cut(s) 1351, 3328
BspTNI GGTCTC 2 cut(s) 2428, 2657
BsrBI CCGCTC 1 cut(s) 2208
BsrDI GCAATG 3 cut(s) 2322, 3168, 3236
BsrFI RCCGGY 2 cut(s) 2482, 2984
BsrGI TGTACA 1 cut(s) 2697
BsrI ACTGG 5 cut(s) 46, 145, 1943, 1971, 2052
BssAI RCCGGY 2 cut(s) 2482, 2984
BssECI CCNNGG 9 cut(s) 51, 485, 494, 1086, 1222, 1773, 2825, 2826, 2837
BssNI GRCGYC 3 cut(s) 84, 1033, 3138
BssSI CACGAG 2 cut(s) 421, 1065
BssT1I CCWWGG 2 cut(s) 1086, 1773
Bst2BI CACGAG 2 cut(s) 421, 1065
Bst2UI CCWGG 7 cut(s) 813, 1029, 1223, 2508, 2827, 3084, 3264
Bst6I CTCTTC 4 cut(s) 392, 920, 981, 1572
BstACI GRCGYC 3 cut(s) 84, 1033, 3138
BstAPI GCANNNNNTGC 5 cut(s) 1019, 1904, 2621, 3418, 3469
BstAUI TGTACA 1 cut(s) 2697
BstBAI YACGTR 1 cut(s) 3017
BstBI TTCGAA 1 cut(s) 444
BstC8I GCNNGC 5 cut(s) 1755, 1834, 2289, 2907, 3470
BstDSI CCRYGG 2 cut(s) 485, 2837
BstEII GGTNACC 1 cut(s) 2980
BstENI CCTNNNNNAGG 1 cut(s) 2199
BstF5I GGATG 5 cut(s) 40, 411, 481, 1024, 3490
BstFNI CGCG 1 cut(s) 301
BstH2I RGCGCY 3 cut(s) 413, 431, 3126
BstHHI GCGC 4 cut(s) 412, 430, 1024, 3125
BstMAI GTCTC 8 cut(s) 131, 531, 992, 1155, 2090, 2180, 2428, 2657
BstNI CCWGG 7 cut(s) 813, 1029, 1223, 2508, 2827, 3084, 3264
BstNSI RCATGY 2 cut(s) 2682, 2717
BstPI GGTNACC 1 cut(s) 2980
BstSFI CTRYAG 6 cut(s) 1427, 1470, 1755, 2412, 2442, 2601
BstSLI GKGCMC 2 cut(s) 501, 3151
BstUI CGCG 1 cut(s) 301
BstV2I GAAGAC 2 cut(s) 1124, 3147
BstX2I RGATCY 6 cut(s) 149, 873, 1423, 1672, 2306, 2848
BstXI CCANNNNNNTGG 2 cut(s) 2560, 3175
BstYI RGATCY 6 cut(s) 149, 873, 1423, 1672, 2306, 2848
Bsu36I CCTNAGG 1 cut(s) 1882
BsuI GTATCC 2 cut(s) 1930, 3463
BsuRI GGCC 8 cut(s) 50, 493, 1227, 1753, 2066, 2205, 2426, 2635
BtgI CCRYGG 2 cut(s) 485, 2837
BtsCI GGATG 5 cut(s) 40, 411, 481, 1024, 3490
BtsI GCAGTG 2 cut(s) 825, 2928
BtsIMutI CAGTG 5 cut(s) 39, 349, 825, 2770, 2928
BveI ACCTGC 2 cut(s) 2608, 2968
Cac8I GCNNGC 5 cut(s) 1755, 1834, 2289, 2907, 3470
CaiI CAGNNNCTG 1 cut(s) 2189
CciI TCATGA 1 cut(s) 3075
CfoI GCGC 4 cut(s) 412, 430, 1024, 3125
Cfr10I RCCGGY 2 cut(s) 2482, 2984
CseI GACGC 2 cut(s) 73, 1041
Csp6I GTAC 9 cut(s) 177, 781, 1003, 1044, 1512, 1524, 1874, 2698, 3487
CspAI ACCGGT 2 cut(s) 2482, 2984
CviAII CATG 7 cut(s) 1913, 2012, 2299, 2679, 2714, 3028, 3076
CviQI GTAC 9 cut(s) 177, 781, 1003, 1044, 1512, 1524, 1874, 2698, 3487
DraIII CACNNNGTG 1 cut(s) 914
EaeI YGGCCR 1 cut(s) 491
Eam1104I CTCTTC 4 cut(s) 392, 920, 981, 1572
EarI CTCTTC 4 cut(s) 392, 920, 981, 1572
EciI GGCGGA 4 cut(s) 159, 553, 1129, 2347
Ecl136II GAGCTC 1 cut(s) 121
Eco130I CCWWGG 2 cut(s) 1086, 1773
Eco24I GRGCYC 2 cut(s) 123, 2553
Eco31I GGTCTC 2 cut(s) 2428, 2657
Eco47I GGWCC 8 cut(s) 1082, 1147, 1265, 1460, 1500, 2142, 2527, 3266
Eco47III AGCGCT 1 cut(s) 3124
Eco53kI GAGCTC 1 cut(s) 121
Eco57I CTGAAG 7 cut(s) 695, 1119, 1757, 2630, 2746, 2951, 2963
Eco72I CACGTG 1 cut(s) 3017
Eco81I CCTNAGG 1 cut(s) 1882
Eco88I CYCGRG 1 cut(s) 1374
Eco91I GGTNACC 1 cut(s) 2980
EcoICRI GAGCTC 1 cut(s) 121
EcoNI CCTNNNNNAGG 1 cut(s) 2199
EcoO109I RGGNCCY 2 cut(s) 1460, 2065
EcoO65I GGTNACC 1 cut(s) 2980
EcoRI GAATTC 1 cut(s) 231
EcoRII CCWGG 7 cut(s) 811, 1027, 1221, 2506, 2825, 3082, 3262
EcoT14I CCWWGG 2 cut(s) 1086, 1773
EcoT38I GRGCYC 2 cut(s) 123, 2553
ErhI CCWWGG 2 cut(s) 1086, 1773
Esp3I CGTCTC 2 cut(s) 531, 992
FaeI CATG 7 cut(s) 1916, 2015, 2302, 2682, 2717, 3031, 3079
FalI AAGNNNNNCTT 2 cut(s) 2514, 2546
FaqI GGGAC 6 cut(s) 1068, 1278, 1513, 2128, 2843, 3022
FatI CATG 7 cut(s) 1912, 2011, 2298, 2678, 2713, 3027, 3075
FauI CCCGC 1 cut(s) 294
FbaI TGATCA 3 cut(s) 919, 1345, 3067
FblI GTMKAC 3 cut(s) 240, 2151, 3141
FokI GGATG 5 cut(s) 47, 418, 468, 1011, 3477
FriOI GRGCYC 2 cut(s) 123, 2553
FspAI RTGCGCAY 1 cut(s) 1023
FspI TGCGCA 1 cut(s) 1023
GlaI GCGC 4 cut(s) 411, 429, 1023, 3124
GsaI CCCAGC 1 cut(s) 2331
GsuI CTGGAG 2 cut(s) 63, 1038
HaeII RGCGCY 3 cut(s) 413, 431, 3126
HaeIII GGCC 8 cut(s) 50, 493, 1227, 1753, 2066, 2205, 2426, 2635
HapII CCGG 7 cut(s) 494, 605, 800, 2140, 2483, 2683, 2985
HgaI GACGC 2 cut(s) 73, 1041
HhaI GCGC 4 cut(s) 412, 430, 1024, 3125
Hin1I GRCGYC 3 cut(s) 84, 1033, 3138
Hin1II CATG 7 cut(s) 1916, 2015, 2302, 2682, 2717, 3031, 3079
Hin6I GCGC 4 cut(s) 410, 428, 1022, 3123
HinP1I GCGC 4 cut(s) 410, 428, 1022, 3123
HincII GTYRAC 2 cut(s) 241, 3142
HindII GTYRAC 2 cut(s) 241, 3142
HpaII CCGG 7 cut(s) 494, 605, 800, 2140, 2483, 2683, 2985
Hpy166II GTNNAC 6 cut(s) 241, 1198, 2152, 3142, 3149, 3269
Hpy188III TCNNGA 8 cut(s) 228, 1313, 1323, 1596, 1866, 2783, 2801, 3076
Hpy8I GTNNAC 6 cut(s) 241, 1198, 2152, 3142, 3149, 3269
Hpy99I CGWCG 6 cut(s) 89, 2956, 3143, 3146, 3404, 3449
Hsp92I GRCGYC 3 cut(s) 84, 1033, 3138
Hsp92II CATG 7 cut(s) 1916, 2015, 2302, 2682, 2717, 3031, 3079
HspAI GCGC 4 cut(s) 410, 428, 1022, 3123
Ksp22I TGATCA 3 cut(s) 919, 1345, 3067
LguI GCTCTTC 1 cut(s) 920
LweI GCATC 8 cut(s) 25, 1033, 1381, 2543, 2698, 2888, 3069, 3408
MbiI CCGCTC 1 cut(s) 2208
MfeI CAATTG 2 cut(s) 1683, 2112
MflI RGATCY 6 cut(s) 149, 873, 1423, 1672, 2306, 2848
MhlI GDGCHC 7 cut(s) 123, 354, 501, 767, 2344, 2553, 3151
MlyI GAGTC 3 cut(s) 1772, 2441, 2972
MmeI TCCRAC 6 cut(s) 172, 1085, 1648, 1975, 3245, 3480
MroXI GAANNNNTTC 1 cut(s) 1611
MslI CAYNNNNRTG 3 cut(s) 345, 770, 3173
MspA1I CMGCKG 2 cut(s) 2902, 2929
MspI CCGG 7 cut(s) 494, 605, 800, 2140, 2483, 2683, 2985
MunI CAATTG 2 cut(s) 1683, 2112
Mva1269I GAATGC 2 cut(s) 751, 3316
MvaI CCWGG 7 cut(s) 813, 1029, 1223, 2508, 2827, 3084, 3264
MvnI CGCG 1 cut(s) 301
NciI CCSGG 3 cut(s) 495, 605, 2684
NlaIII CATG 7 cut(s) 1916, 2015, 2302, 2682, 2717, 3031, 3079
NmeAIII GCCGAG 2 cut(s) 76, 628
NmuCI GTSAC 7 cut(s) 659, 903, 1963, 2172, 2496, 2816, 3347
NsbI TGCGCA 1 cut(s) 1023
NspI RCATGY 2 cut(s) 2682, 2717
NspV TTCGAA 1 cut(s) 444
OliI CACNNNNGTG 2 cut(s) 345, 770
PagI TCATGA 1 cut(s) 3075
PaqCI CACCTGC 1 cut(s) 2608
PasI CCCWGGG 1 cut(s) 2826
PciSI GCTCTTC 1 cut(s) 920
PcsI WCGNNNNNNNCGW 2 cut(s) 597, 1039
PctI GAATGC 2 cut(s) 751, 3316
PdmI GAANNNNTTC 1 cut(s) 1611
PfeI GAWTC 8 cut(s) 125, 215, 320, 706, 832, 1988, 2471, 2938
Pfl23II CGTACG 1 cut(s) 1002
PflMI CCANNNNNTGG 1 cut(s) 2858
PfoI TCCNGGA 1 cut(s) 1027
PinAI ACCGGT 2 cut(s) 2482, 2984
PleI GAGTC 3 cut(s) 1772, 2441, 2971
PmaCI CACGTG 1 cut(s) 3017
PmlI CACGTG 1 cut(s) 3017
PpsI GAGTC 3 cut(s) 1772, 2441, 2971
Ppu21I YACGTR 1 cut(s) 3017
PpuMI RGGWCCY 1 cut(s) 1460
Psp124BI GAGCTC 1 cut(s) 123
Psp5II RGGWCCY 1 cut(s) 1460
Psp6I CCWGG 7 cut(s) 811, 1027, 1221, 2506, 2825, 3082, 3262
PspCI CACGTG 1 cut(s) 3017
PspEI GGTNACC 1 cut(s) 2980
PspFI CCCAGC 1 cut(s) 2327
PspGI CCWGG 7 cut(s) 811, 1027, 1221, 2506, 2825, 3082, 3262
PspLI CGTACG 1 cut(s) 1002
PspPPI RGGWCCY 1 cut(s) 1460
PstI CTGCAG 4 cut(s) 1431, 1759, 2416, 2605
PstNI CAGNNNCTG 1 cut(s) 2189
PsuI RGATCY 6 cut(s) 149, 873, 1423, 1672, 2306, 2848
PvuII CAGCTG 1 cut(s) 2929
RsaI GTAC 9 cut(s) 178, 782, 1004, 1045, 1513, 1525, 1875, 2699, 3488
RsaNI GTAC 9 cut(s) 177, 781, 1003, 1044, 1512, 1524, 1874, 2698, 3487
RseI CAYNNNNRTG 3 cut(s) 345, 770, 3173
SacI GAGCTC 1 cut(s) 123
SalI GTCGAC 2 cut(s) 239, 3140
SapI GCTCTTC 1 cut(s) 920
SchI GAGTC 3 cut(s) 1772, 2441, 2972
SduI GDGCHC 7 cut(s) 123, 354, 501, 767, 2344, 2553, 3151
SfaNI GCATC 8 cut(s) 25, 1033, 1381, 2543, 2698, 2888, 3069, 3408
SfcI CTRYAG 6 cut(s) 1427, 1470, 1755, 2412, 2442, 2601
SfuI TTCGAA 1 cut(s) 444
SgrDI CGTCGACG 1 cut(s) 3140
SinI GGWCC 8 cut(s) 1082, 1147, 1265, 1460, 1500, 2142, 2527, 3266
SmiMI CAYNNNNRTG 3 cut(s) 345, 770, 3173
SmlI CTYRAG 1 cut(s) 2501
SmoI CTYRAG 1 cut(s) 2501
SpeI ACTAGT 2 cut(s) 3047, 3344
SstI GAGCTC 1 cut(s) 123
StyI CCWWGG 2 cut(s) 1086, 1773
TatI WGTACW 1 cut(s) 2697
TauI GCSGC 4 cut(s) 84, 538, 2208, 2905
TfiI GAWTC 8 cut(s) 125, 215, 320, 706, 832, 1988, 2471, 2938
TscAI CASTG 5 cut(s) 46, 349, 825, 2770, 2928
TseFI GTSAC 7 cut(s) 659, 903, 1963, 2172, 2496, 2816, 3347
Tsp45I GTSAC 7 cut(s) 659, 903, 1963, 2172, 2496, 2816, 3347
TspGWI ACGGA 4 cut(s) 194, 474, 559, 2645
TspRI CASTG 5 cut(s) 46, 349, 825, 2770, 2928
Van91I CCANNNNNTGG 1 cut(s) 2858
VneI GTGCAC 1 cut(s) 3147
VpaK11BI GGWCC 8 cut(s) 1082, 1147, 1265, 1460, 1500, 2142, 2527, 3266
XagI CCTNNNNNAGG 1 cut(s) 2199
XapI RAATTY 4 cut(s) 231, 329, 2778, 3023
XceI RCATGY 2 cut(s) 2682, 2717
XmiI GTMKAC 3 cut(s) 240, 2151, 3141
XmnI GAANNNNTTC 1 cut(s) 1611
ZraI GACGTC 1 cut(s) 3139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.