Rmu_sc0034256.1_g000001

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0034256.1
OG0010008
Physical Location & Seq
Forward (+)
1 .. 762
762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0034256.1_g000001.1.cds

Sequence Viewer

Length: 762 bp
aaggaagctgaacctaaggacggcggcgttggtagccccgaggatgaggctagggtttcgccaatggagctcgatttagaggccgagggcggcggttctatgggctccgagggcgggagatctgatagagttagggttagtccggagagtggcgatggaaatgtagataagggaatggaatcgaaagggtttaaaattcagggcggcgatggattgggtaacgatgatattgatgacgtgtttgatattgcgagagttgaaattgatgatcaggatgaggatagctctgaggctcagaatgagagtgtggatttgttatctgagtttgatgagtatgtggctaatgagaaggacggaatggctttgggaacctccagagctctgagctatggtttcgaagtcggtgatatggtgtgggggaaggtcaagtctcatccctggtggccggggcatatatttaatgaggcctttgccacgtctcaggtgcgccggacacggagggaaggtcatgtcctggtggctttctttggtgatagtagctacggctggttcgacccggccgagcttatcccgttcgagccccatttcgcagagaagtctcggcagacgaattacaggaattttaacagggctgtagaggaggctgttgatgaggcgagtcgcaggtgtggagttggtttcgtgtgtaagtgtaggaatccgtataattttcgggccactagtgtgccggggtactttgttgttgatgtgccggattatgag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
PWWP

Protein Analysis

254

Amino Acids

28.05

Weight (kDa)

4.46

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 654
Acc36I ACCTGC 1 cut(s) 654
AccIII TCCGGA 1 cut(s) 142
AciI CCGC 5 cut(s) 24, 90, 93, 114, 204
AcoI YGGCCR 2 cut(s) 443, 558
AcsI RAATTY 2 cut(s) 195, 619
AfaI GTAC 1 cut(s) 734
AfiI CCNNNNNNNGG 3 cut(s) 20, 114, 149
AflIII ACRYGT 1 cut(s) 237
AgsI TTSAA 1 cut(s) 260
AhlI ACTAGT 1 cut(s) 719
AjiI CACGTC 2 cut(s) 238, 477
AjnI CCWGG 2 cut(s) 437, 513
AleI CACNNNNGTG 1 cut(s) 722
AluBI AGCT 7 cut(s) 8, 70, 285, 380, 387, 540, 565
AluI AGCT 7 cut(s) 8, 70, 285, 380, 387, 540, 565
Alw21I GWGCWC 2 cut(s) 72, 382
Alw26I GTCTC 3 cut(s) 435, 483, 603
Ama87I CYCGRG 1 cut(s) 38
Aor13HI TCCGGA 1 cut(s) 142
AoxI GGCC 5 cut(s) 81, 443, 465, 558, 714
ApoI RAATTY 2 cut(s) 195, 619
AspLEI GCGC 1 cut(s) 489
AspS9I GGNCC 1 cut(s) 714
AsuC2I CCSGG 3 cut(s) 447, 557, 729
AsuHPI GGTGA 2 cut(s) 416, 542
AsuII TTCGAA 1 cut(s) 396
AvaI CYCGRG 1 cut(s) 38
AxyI CCTNAGG 1 cut(s) 15
BanII GRGCYC 4 cut(s) 72, 107, 382, 582
Bbv12I GWGCWC 2 cut(s) 72, 382
BccI CCATC 2 cut(s) 149, 203
BceAI ACGGC 2 cut(s) 37, 559
BciT130I CCWGG 2 cut(s) 439, 515
BclI TGATCA 1 cut(s) 268
BcnI CCSGG 3 cut(s) 447, 557, 729
BcoDI GTCTC 3 cut(s) 435, 483, 603
BcuI ACTAGT 1 cut(s) 719
BfaI CTAG 2 cut(s) 51, 720
BfmI CTRYAG 1 cut(s) 633
BfuAI ACCTGC 1 cut(s) 654
BglII AGATCT 1 cut(s) 119
BisI GCNGC 3 cut(s) 25, 91, 205
BlsI GCNGC 3 cut(s) 26, 92, 206
Bme1390I CCNGG 5 cut(s) 439, 447, 515, 557, 729
BmeT110I CYCGRG 1 cut(s) 38
BmgBI CACGTC 2 cut(s) 238, 477
BmgT120I GGNCC 1 cut(s) 714
BmiI GGNNCC 2 cut(s) 106, 370
BmrFI CCNGG 5 cut(s) 439, 447, 515, 557, 729
BplI GAGNNNNNCTC 2 cut(s) 269, 301
BpmI CTGGAG 1 cut(s) 358
Bpu14I TTCGAA 1 cut(s) 396
BpuMI CCSGG 3 cut(s) 447, 557, 729
BsaJI CCNNGG 6 cut(s) 39, 84, 108, 437, 446, 728
BsaWI WCCGGW 1 cut(s) 142
BsaXI ACNNNNNCTCC 2 cut(s) 490, 520
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 114, 149
Bse21I CCTNAGG 1 cut(s) 15
BseAI TCCGGA 1 cut(s) 142
BseBI CCWGG 2 cut(s) 439, 515
BseDI CCNNGG 6 cut(s) 39, 84, 108, 437, 446, 728
BseGI GGATG 3 cut(s) 49, 280, 433
BseLI CCNNNNNNNGG 3 cut(s) 20, 114, 149
BseMII CTCAG 5 cut(s) 279, 308, 312, 374, 494
BseRI GAGGAG 1 cut(s) 653
BseX3I CGGCCG 1 cut(s) 558
Bsh1285I CGRYCG 1 cut(s) 561
BshFI GGCC 5 cut(s) 83, 445, 467, 560, 716
BsiEI CGRYCG 1 cut(s) 561
BsiHKAI GWGCWC 2 cut(s) 72, 382
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 6 cut(s) 143, 446, 490, 557, 728, 752
BslI CCNNNNNNNGG 3 cut(s) 20, 114, 149
BsmAI GTCTC 3 cut(s) 435, 483, 603
BsmBI CGTCTC 1 cut(s) 483
BsnI GGCC 5 cut(s) 83, 445, 467, 560, 716
BsoBI CYCGRG 1 cut(s) 38
Bsp119I TTCGAA 1 cut(s) 396
Bsp1286I GDGCHC 4 cut(s) 72, 107, 382, 582
Bsp13I TCCGGA 1 cut(s) 142
Bsp143I GATC 2 cut(s) 119, 268
BspACI CCGC 5 cut(s) 24, 90, 93, 114, 204
BspANI GGCC 5 cut(s) 83, 445, 467, 560, 716
BspCNI CTCAG 5 cut(s) 280, 307, 313, 375, 493
BspEI TCCGGA 1 cut(s) 142
BspLI GGNNCC 2 cut(s) 106, 370
BspMI ACCTGC 1 cut(s) 654
BspT104I TTCGAA 1 cut(s) 396
BssECI CCNNGG 6 cut(s) 39, 84, 108, 437, 446, 728
BssMI GATC 2 cut(s) 119, 268
Bst2UI CCWGG 2 cut(s) 439, 515
BstBI TTCGAA 1 cut(s) 396
BstDEI CTNAG 6 cut(s) 15, 288, 294, 321, 383, 480
BstF5I GGATG 3 cut(s) 49, 280, 433
BstHHI GCGC 1 cut(s) 489
BstKTI GATC 2 cut(s) 122, 271
BstMAI GTCTC 3 cut(s) 435, 483, 603
BstMBI GATC 2 cut(s) 119, 268
BstMCI CGRYCG 1 cut(s) 561
BstMWI GCNNNNNNNGC 3 cut(s) 33, 67, 111
BstNI CCWGG 2 cut(s) 439, 515
BstSCI CCNGG 5 cut(s) 437, 445, 513, 555, 727
BstSFI CTRYAG 1 cut(s) 633
BstX2I RGATCY 1 cut(s) 119
BstYI RGATCY 1 cut(s) 119
BstZI CGGCCG 1 cut(s) 558
Bsu36I CCTNAGG 1 cut(s) 15
BsuRI GGCC 5 cut(s) 83, 445, 467, 560, 716
BtgZI GCGATG 2 cut(s) 168, 222
BtrI CACGTC 2 cut(s) 238, 477
BtsCI GGATG 3 cut(s) 49, 280, 433
BveI ACCTGC 1 cut(s) 654
CfoI GCGC 1 cut(s) 489
Cfr13I GGNCC 1 cut(s) 714
Csp6I GTAC 1 cut(s) 733
CviAII CATG 1 cut(s) 509
CviQI GTAC 1 cut(s) 733
DdeI CTNAG 6 cut(s) 15, 288, 294, 321, 383, 480
DpnI GATC 2 cut(s) 121, 270
DpnII GATC 2 cut(s) 119, 268
DraI TTTAAA 1 cut(s) 193
EaeI YGGCCR 2 cut(s) 443, 558
EagI CGGCCG 1 cut(s) 558
Ecl136II GAGCTC 2 cut(s) 70, 380
EclXI CGGCCG 1 cut(s) 558
Eco147I AGGCCT 1 cut(s) 467
Eco24I GRGCYC 4 cut(s) 72, 107, 382, 582
Eco52I CGGCCG 1 cut(s) 558
Eco53kI GAGCTC 2 cut(s) 70, 380
Eco81I CCTNAGG 1 cut(s) 15
Eco88I CYCGRG 1 cut(s) 38
EcoICRI GAGCTC 2 cut(s) 70, 380
EcoRII CCWGG 2 cut(s) 437, 513
EcoT38I GRGCYC 4 cut(s) 72, 107, 382, 582
Esp3I CGTCTC 1 cut(s) 483
FaeI CATG 1 cut(s) 512
FaiI YATR 9 cut(s) 101, 336, 390, 410, 453, 455, 510, 705, 759
FatI CATG 1 cut(s) 508
FauI CCCGC 1 cut(s) 107
FbaI TGATCA 1 cut(s) 268
Fnu4HI GCNGC 3 cut(s) 25, 91, 205
FokI GGATG 3 cut(s) 56, 287, 420
FriOI GRGCYC 4 cut(s) 72, 107, 382, 582
Fsp4HI GCNGC 3 cut(s) 25, 91, 205
FspBI CTAG 2 cut(s) 51, 720
GlaI GCGC 1 cut(s) 488
GluI GCNGC 3 cut(s) 25, 91, 205
GsuI CTGGAG 1 cut(s) 358
HaeIII GGCC 5 cut(s) 83, 445, 467, 560, 716
HapII CCGG 6 cut(s) 143, 446, 490, 557, 728, 752
HhaI GCGC 1 cut(s) 489
Hin1II CATG 1 cut(s) 512
Hin6I GCGC 1 cut(s) 487
HinP1I GCGC 1 cut(s) 487
HinfI GANTC 3 cut(s) 179, 658, 697
HpaII CCGG 6 cut(s) 143, 446, 490, 557, 728, 752
HphI GGTGA 2 cut(s) 416, 542
Hpy188I TCNGA 6 cut(s) 109, 124, 289, 297, 322, 384
Hpy188III TCNNGA 3 cut(s) 143, 272, 375
HpyAV CCTTC 3 cut(s) 343, 415, 497
HpyCH4IV ACGT 2 cut(s) 237, 476
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 67, 111
HpyF3I CTNAG 6 cut(s) 15, 288, 294, 321, 383, 480
HpySE526I ACGT 2 cut(s) 237, 476
Hsp92II CATG 1 cut(s) 512
HspAI GCGC 1 cut(s) 487
Kpn2I TCCGGA 1 cut(s) 142
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 2 cut(s) 119, 268
LmnI GCTCC 2 cut(s) 67, 110
MaeI CTAG 2 cut(s) 51, 720
MaeII ACGT 2 cut(s) 237, 476
MaeIII GTNAC 1 cut(s) 218
MalI GATC 2 cut(s) 121, 270
MboI GATC 2 cut(s) 119, 268
MflI RGATCY 1 cut(s) 119
MhlI GDGCHC 4 cut(s) 72, 107, 382, 582
MluCI AATT 5 cut(s) 195, 261, 610, 619, 706
MlyI GAGTC 1 cut(s) 667
MroI TCCGGA 1 cut(s) 142
MseI TTAA 3 cut(s) 192, 459, 624
MslI CAYNNNNRTG 1 cut(s) 722
MspI CCGG 6 cut(s) 143, 446, 490, 557, 728, 752
MspR9I CCNGG 5 cut(s) 439, 447, 515, 557, 729
MvaI CCWGG 2 cut(s) 439, 515
MwoI GCNNNNNNNGC 3 cut(s) 33, 67, 111
NciI CCSGG 3 cut(s) 447, 557, 729
NdeII GATC 2 cut(s) 119, 268
NlaIII CATG 1 cut(s) 512
NlaIV GGNNCC 2 cut(s) 106, 370
NmeAIII GCCGAG 3 cut(s) 109, 580, 586
NspV TTCGAA 1 cut(s) 396
OliI CACNNNNGTG 1 cut(s) 722
PaqCI CACCTGC 1 cut(s) 654
PceI AGGCCT 1 cut(s) 467
PcsI WCGNNNNNNNCGW 2 cut(s) 549, 558
PfeI GAWTC 2 cut(s) 179, 697
PkrI GCNGC 3 cut(s) 26, 92, 206
PleI GAGTC 1 cut(s) 666
PpsI GAGTC 1 cut(s) 666
Psp124BI GAGCTC 2 cut(s) 72, 382
Psp6I CCWGG 2 cut(s) 437, 513
PspGI CCWGG 2 cut(s) 437, 513
PspN4I GGNNCC 2 cut(s) 106, 370
PspPI GGNCC 1 cut(s) 714
PsuI RGATCY 1 cut(s) 119
RsaI GTAC 1 cut(s) 734
RsaNI GTAC 1 cut(s) 733
RseI CAYNNNNRTG 1 cut(s) 722
SacI GAGCTC 2 cut(s) 72, 382
SaqAI TTAA 3 cut(s) 192, 459, 624
SatI GCNGC 3 cut(s) 25, 91, 205
Sau3AI GATC 2 cut(s) 119, 268
Sau96I GGNCC 1 cut(s) 714
SchI GAGTC 1 cut(s) 667
ScrFI CCNGG 5 cut(s) 439, 447, 515, 557, 729
SduI GDGCHC 4 cut(s) 72, 107, 382, 582
SfcI CTRYAG 1 cut(s) 633
SfuI TTCGAA 1 cut(s) 396
SmiMI CAYNNNNRTG 1 cut(s) 722
SpeI ACTAGT 1 cut(s) 719
Sse9I AATT 5 cut(s) 195, 261, 610, 619, 706
SseBI AGGCCT 1 cut(s) 467
SsiI CCGC 5 cut(s) 24, 90, 93, 114, 204
SspMI CTAG 2 cut(s) 51, 720
SstI GAGCTC 2 cut(s) 72, 382
StuI AGGCCT 1 cut(s) 467
StyD4I CCNGG 5 cut(s) 437, 445, 513, 555, 727
TaiI ACGT 2 cut(s) 240, 479
TaqI TCGA 5 cut(s) 72, 182, 396, 552, 576
TasI AATT 5 cut(s) 195, 261, 610, 619, 706
TauI GCSGC 3 cut(s) 27, 93, 207
TfiI GAWTC 2 cut(s) 179, 697
Tru1I TTAA 3 cut(s) 192, 459, 624
Tru9I TTAA 3 cut(s) 192, 459, 624
TspGWI ACGGA 3 cut(s) 369, 511, 690
XapI RAATTY 2 cut(s) 195, 619
XspI CTAG 2 cut(s) 51, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.