FvH4_6g08130

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015816
Physical Location & Seq
Reverse (-)
4830809 .. 4833103
2295 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g08130.t1

Sequence Viewer

Length: 1380 bp
ATGCTGATCAACTTTGTTCTTGCTCATATTTTCAACTTCTTTCTCTGTTTCTTGTTTCTTCCAATACACGGCAGACTTCACTACACTTCTTCACTTGTAGTGTCTCAAATTTCAGTGCCGCCTTCTCCTGCACCCGCGGCTGCTGTTAGCCCGACTTACAACGGTAGTTCCGCTCTTGGTACTACTGTTTTCAATGTGAGAACTTTTGGTGCAGTTGGGAATGGTGTTACTGATGATACAGAAGCATTTAAAATGGCTTGGGACAGTGCTTGTCAAACTGAGAATTCGGAAGTTCTTGTTCCAAAAAGTCGTTCCTATATGTTACAGTCTACCATATTCACAGGGCCATGCAAATCTGGCCTCAAACTGCAGATTGATGGGACATTAGTTCCACCAGATGGACCGGATTCATGGCCGAAAAAAATCAGTAAGAGACAGTGGTTGGTTTTCTACAGAATCAATGGAATGTCGATGCAAGGAGATGGTGTAATAGATGGTAGAGGAGAGAAATGGTGGAATCTTCCTTGCAAACCCCACAAAGGACCCCATGGAACAACACTGCCTGGACCTTGTGATAGCCCGACTGCTATAAGGTTCTTCATGTGCTCTAATCTGAAACTGAAAGGACTCAGAATCAAGAACAGCCCCCAGTTTCATTTCAAGTTCGACAATTGTCAAAACGTTTACGTAGAATCACTTACCATAAAATCACCTGCTCTTAGTCCCAACACAGATGGAATTCACATTCAGAACACAAACAATATGAAAATATACAACTCACTCGTGTCCAATGGTGATGATTGTGTATCAATTGGAACTGGTTCTTATAATATTGACATTAGGAACATTACTTGTGGTCCAAGTCATGGAATAAGCATTGGAAGTCTTGGAGACGGCAATTCCCGAGCATGTGTTTCGAATATCACAGTAAAAGACTCGATCATCAAGCACTCTGACAATGGGGTTCGGATCAAAACATGGCAAGGTGGATCAGGATCTGTGTCAAGCATAACATTCAACAACATTCACATGGACACCGTAAGAAATCCGATTGTGCTAGACCAATACTACTGCCTCACCAAGCATTGCCCTAACCAAACCTCTGCAGTTCACATATCAAATATCTTGTACACAAACATAAGGGGTACTTATGATGTGAGAAGCCCCCCTATGCATTTCGCTTGCAGTGACTCGATGCCTTGTACAAACCTCACTCTCTCTGAAGTGGAATTGCTCCCTGCTACCAAAGGACACTTAGTGCCGATACCGCTTTGCTGGAGTGCTTATGGAACTATGCAGACAATTACTGTTCCACCAGTTTTGTGCTTGTTAGAGGGCATCCCAGAATTGATGCCCCAAAATGATAGTGATAGGTGTTAA

Protein Analysis

460

Amino Acids

50.15

Weight (kDa)

8.25

Isoelectric Point (pI)

49.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 93 - 411 7.1e-93 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015816)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56710
fragaria_vesca FvH4_6g08130
malus_domestica MD04G1189600.v1.1
prunus_persica Prupe.6G307400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0457981
rosa_laevigata RLG00000025181
rosa_multiflora Rmu_sc0000384.1_g000005
rosa_roxburghii Rroxscaffold_6G00421410
rosa_rugosa Rorug03G0024000
rosa_samantha Rh3AG084600 Rh3BG087300 Rh3CG087800 Rh3DG088100
rosa_wichuraiana Rw0G012380 Rw3G007200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 828
AarI CACCTGC 1 cut(s) 721
Acc36I ACCTGC 1 cut(s) 721
AccB7I CCANNNNNTGG 2 cut(s) 398, 866
AccBSI CCGCTC 1 cut(s) 173
AccI GTMKAC 1 cut(s) 329
AccII CGCG 1 cut(s) 137
AciI CCGC 5 cut(s) 119, 135, 137, 171, 1268
AclI AACGTT 1 cut(s) 681
AclWI GGATC 3 cut(s) 977, 997, 1003
AcoI YGGCCR 1 cut(s) 413
AcsI RAATTY 3 cut(s) 108, 283, 738
AcuI CTGAAG 1 cut(s) 1242
AdeI CACNNNGTG 1 cut(s) 1258
AfaI GTAC 4 cut(s) 181, 1130, 1147, 1204
AfiI CCNNNNNNNGG 4 cut(s) 68, 398, 539, 866
AgsI TTSAA 4 cut(s) 34, 193, 661, 1018
AjnI CCWGG 1 cut(s) 562
AjuI GAANNNNNNNTTGG 2 cut(s) 295, 327
Alw21I GWGCWC 1 cut(s) 608
Alw26I GTCTC 3 cut(s) 108, 427, 885
AlwI GGATC 3 cut(s) 977, 997, 1003
AlwNI CAGNNNCTG 1 cut(s) 998
Ama87I CYCGRG 1 cut(s) 903
AoxI GGCC 3 cut(s) 344, 358, 413
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 3 cut(s) 108, 283, 738
Asp700I GAANNNNTTC 1 cut(s) 820
AspS9I GGNCC 5 cut(s) 344, 401, 542, 566, 857
AsuHPI GGTGA 3 cut(s) 702, 806, 1069
AsuII TTCGAA 1 cut(s) 917
AvaI CYCGRG 1 cut(s) 903
AvaII GGWCC 4 cut(s) 401, 542, 566, 857
BaeI ACNNNNGTAYC 2 cut(s) 171, 204
BauI CACGAG 1 cut(s) 782
Bbv12I GWGCWC 1 cut(s) 608
BbvI GCAGC 1 cut(s) 127
BccI CCATC 5 cut(s) 371, 392, 476, 488, 728
BceAI ACGGC 2 cut(s) 85, 910
BcgI CGANNNNNNTGC 2 cut(s) 897, 931
BciT130I CCWGG 1 cut(s) 564
BclI TGATCA 1 cut(s) 6
BcoDI GTCTC 3 cut(s) 108, 427, 885
BfaI CTAG 1 cut(s) 1058
BfmI CTRYAG 3 cut(s) 368, 451, 1104
BfuAI ACCTGC 1 cut(s) 721
BisI GCNGC 3 cut(s) 119, 138, 141
BlsI GCNGC 3 cut(s) 120, 139, 142
Bme1390I CCNGG 1 cut(s) 564
Bme18I GGWCC 4 cut(s) 401, 542, 566, 857
BmeT110I CYCGRG 1 cut(s) 903
BmgT120I GGNCC 5 cut(s) 344, 401, 542, 566, 857
BmiI GGNNCC 1 cut(s) 544
BmrFI CCNGG 1 cut(s) 564
BmrI ACTGGG 1 cut(s) 643
BmsI GCATC 4 cut(s) 462, 1185, 1341, 1347
BmuI ACTGGG 1 cut(s) 643
BoxI GACNNNNGTC 1 cut(s) 672
BpmI CTGGAG 1 cut(s) 1297
Bpu14I TTCGAA 1 cut(s) 917
BsaAI YACGTR 1 cut(s) 688
BsaBI GATNNNNATC 1 cut(s) 994
BsaJI CCNNGG 2 cut(s) 135, 547
BsaWI WCCGGW 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 4 cut(s) 68, 398, 539, 866
Bse1I ACTGG 3 cut(s) 649, 823, 1316
Bse3DI GCAATG 1 cut(s) 1084
Bse8I GATNNNNATC 1 cut(s) 994
BseBI CCWGG 1 cut(s) 564
BseDI CCNNGG 2 cut(s) 135, 547
BseGI GGATG 1 cut(s) 1338
BseJI GATNNNNATC 1 cut(s) 994
BseLI CCNNNNNNNGG 4 cut(s) 68, 398, 539, 866
BseMI GCAATG 1 cut(s) 1084
BseMII CTCAG 2 cut(s) 270, 643
BseNI ACTGG 3 cut(s) 649, 823, 1316
BseRI GAGGAG 1 cut(s) 516
BseXI GCAGC 1 cut(s) 127
BsgI GTGCAG 2 cut(s) 114, 231
Bsh1236I CGCG 1 cut(s) 137
BshFI GGCC 3 cut(s) 346, 360, 415
BsiHKAI GWGCWC 1 cut(s) 608
BsiHKCI CYCGRG 1 cut(s) 903
BsiSI CCGG 1 cut(s) 404
BslFI GGGAC 3 cut(s) 275, 394, 708
BslI CCNNNNNNNGG 4 cut(s) 68, 398, 539, 866
BsmAI GTCTC 3 cut(s) 108, 427, 885
BsmBI CGTCTC 1 cut(s) 885
BsmFI GGGAC 3 cut(s) 275, 394, 708
BsnI GGCC 3 cut(s) 346, 360, 415
BsoBI CYCGRG 1 cut(s) 903
Bsp119I TTCGAA 1 cut(s) 917
Bsp1286I GDGCHC 1 cut(s) 608
Bsp1407I TGTACA 2 cut(s) 1128, 1202
Bsp143I GATC 5 cut(s) 6, 939, 969, 989, 995
Bsp19I CCATGG 1 cut(s) 547
BspACI CCGC 5 cut(s) 119, 135, 137, 171, 1268
BspANI GGCC 3 cut(s) 346, 360, 415
BspCNI CTCAG 2 cut(s) 271, 642
BspFNI CGCG 1 cut(s) 137
BspLI GGNNCC 1 cut(s) 544
BspMAI CTGCAG 2 cut(s) 372, 1108
BspMI ACCTGC 1 cut(s) 721
BspPI GGATC 3 cut(s) 977, 997, 1003
BspT104I TTCGAA 1 cut(s) 917
BsrBI CCGCTC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 1084
BsrGI TGTACA 2 cut(s) 1128, 1202
BsrI ACTGG 3 cut(s) 649, 823, 1316
BssECI CCNNGG 2 cut(s) 135, 547
BssMI GATC 5 cut(s) 6, 939, 969, 989, 995
BssSI CACGAG 1 cut(s) 782
BssT1I CCWWGG 1 cut(s) 547
Bst2BI CACGAG 1 cut(s) 782
Bst2UI CCWGG 1 cut(s) 564
Bst4CI ACNGT 8 cut(s) 164, 187, 266, 327, 438, 928, 1039, 1309
BstAUI TGTACA 2 cut(s) 1128, 1202
BstBAI YACGTR 1 cut(s) 688
BstBI TTCGAA 1 cut(s) 917
BstC8I GCNNGC 1 cut(s) 1183
BstDEI CTNAG 4 cut(s) 279, 629, 719, 1255
BstDSI CCRYGG 2 cut(s) 135, 547
BstF5I GGATG 1 cut(s) 1338
BstFNI CGCG 1 cut(s) 137
BstKTI GATC 5 cut(s) 9, 942, 972, 992, 998
BstMAI GTCTC 3 cut(s) 108, 427, 885
BstMBI GATC 5 cut(s) 6, 939, 969, 989, 995
BstMWI GCNNNNNNNGC 3 cut(s) 137, 357, 1267
BstNI CCWGG 1 cut(s) 564
BstNSI RCATGY 1 cut(s) 912
BstPAI GACNNNNGTC 1 cut(s) 672
BstSCI CCNGG 1 cut(s) 562
BstSFI CTRYAG 3 cut(s) 368, 451, 1104
BstSNI TACGTA 1 cut(s) 688
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 1 cut(s) 127
BstX2I RGATCY 1 cut(s) 995
BstYI RGATCY 1 cut(s) 995
BsuRI GGCC 3 cut(s) 346, 360, 415
BtgI CCRYGG 2 cut(s) 135, 547
BtsCI GGATG 1 cut(s) 1338
BtsI GCAGTG 2 cut(s) 557, 1192
BtsIMutI CAGTG 5 cut(s) 120, 271, 443, 557, 1192
BveI ACCTGC 1 cut(s) 721
Cac8I GCNNGC 1 cut(s) 1183
CaiI CAGNNNCTG 1 cut(s) 998
Cfr13I GGNCC 5 cut(s) 344, 401, 542, 566, 857
Cfr42I CCGCGG 1 cut(s) 138
Csp6I GTAC 4 cut(s) 180, 1129, 1146, 1203
CspCI CAANNNNNGTGG 2 cut(s) 1302, 1337
CviAII CATG 8 cut(s) 348, 411, 548, 601, 866, 909, 978, 1030
CviJI RGCY 9 cut(s) 140, 150, 257, 346, 360, 415, 579, 645, 1164
CviKI_1 RGCY 9 cut(s) 140, 150, 257, 346, 360, 415, 579, 645, 1164
CviQI GTAC 4 cut(s) 180, 1129, 1146, 1203
DdeI CTNAG 4 cut(s) 279, 629, 719, 1255
DpnI GATC 5 cut(s) 8, 941, 971, 991, 997
DpnII GATC 5 cut(s) 6, 939, 969, 989, 995
DraI TTTAAA 1 cut(s) 250
DraIII CACNNNGTG 1 cut(s) 1258
EaeI YGGCCR 1 cut(s) 413
Eco105I TACGTA 1 cut(s) 688
Eco130I CCWWGG 1 cut(s) 547
Eco47I GGWCC 4 cut(s) 401, 542, 566, 857
Eco57I CTGAAG 1 cut(s) 1242
Eco88I CYCGRG 1 cut(s) 903
EcoO109I RGGNCCY 1 cut(s) 542
EcoRI GAATTC 2 cut(s) 283, 738
EcoRII CCWGG 1 cut(s) 562
EcoT14I CCWWGG 1 cut(s) 547
EcoT22I ATGCAT 1 cut(s) 1176
ErhI CCWWGG 1 cut(s) 547
Esp3I CGTCTC 1 cut(s) 885
FaeI CATG 8 cut(s) 351, 414, 551, 604, 869, 912, 981, 1033
FalI AAGNNNNNCTT 2 cut(s) 1132, 1164
FaqI GGGAC 3 cut(s) 275, 394, 708
FatI CATG 8 cut(s) 347, 410, 547, 600, 865, 908, 977, 1029
FauI CCCGC 1 cut(s) 142
FbaI TGATCA 1 cut(s) 6
FblI GTMKAC 1 cut(s) 329
Fnu4HI GCNGC 3 cut(s) 119, 138, 141
FokI GGATG 1 cut(s) 1325
Fsp4HI GCNGC 3 cut(s) 119, 138, 141
FspBI CTAG 1 cut(s) 1058
GluI GCNGC 3 cut(s) 119, 138, 141
GsuI CTGGAG 1 cut(s) 1297
HaeIII GGCC 3 cut(s) 346, 360, 415
HapII CCGG 1 cut(s) 404
Hin1II CATG 8 cut(s) 351, 414, 551, 604, 869, 912, 981, 1033
HinfI GANTC 8 cut(s) 407, 456, 517, 627, 633, 692, 935, 1190
HpaII CCGG 1 cut(s) 404
HphI GGTGA 3 cut(s) 702, 806, 1069
Hpy166II GTNNAC 4 cut(s) 330, 685, 1111, 1131
Hpy188I TCNGA 8 cut(s) 289, 615, 632, 750, 955, 969, 1050, 1222
Hpy188III TCNNGA 3 cut(s) 637, 903, 993
Hpy8I GTNNAC 4 cut(s) 330, 685, 1111, 1131
HpyAV CCTTC 1 cut(s) 132
HpyCH4III ACNGT 8 cut(s) 164, 187, 266, 327, 438, 928, 1039, 1309
HpyCH4IV ACGT 2 cut(s) 681, 687
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 357, 1267
HpyF3I CTNAG 4 cut(s) 279, 629, 719, 1255
HpySE526I ACGT 2 cut(s) 681, 687
Hsp92II CATG 8 cut(s) 351, 414, 551, 604, 869, 912, 981, 1033
Ksp22I TGATCA 1 cut(s) 6
KspI CCGCGG 1 cut(s) 138
Kzo9I GATC 5 cut(s) 6, 939, 969, 989, 995
LmnI GCTCC 1 cut(s) 1239
Lsp1109I GCAGC 1 cut(s) 127
LweI GCATC 4 cut(s) 462, 1185, 1341, 1347
MaeI CTAG 1 cut(s) 1058
MaeII ACGT 2 cut(s) 681, 687
MaeIII GTNAC 3 cut(s) 226, 321, 1187
MalI GATC 5 cut(s) 8, 941, 971, 991, 997
MbiI CCGCTC 1 cut(s) 173
MboI GATC 5 cut(s) 6, 939, 969, 989, 995
MboII GAAGA 4 cut(s) 50, 81, 512, 589
MfeI CAATTG 2 cut(s) 670, 810
MflI RGATCY 1 cut(s) 995
MhlI GDGCHC 1 cut(s) 608
MluCI AATT 9 cut(s) 108, 283, 670, 738, 810, 898, 1229, 1302, 1346
MlyI GAGTC 3 cut(s) 621, 929, 1184
MnlI CCTC 6 cut(s) 371, 494, 1085, 1111, 1220, 1327
Mph1103I ATGCAT 1 cut(s) 1176
MroXI GAANNNNTTC 1 cut(s) 820
MseI TTAA 2 cut(s) 249, 1378
MslI CAYNNNNRTG 1 cut(s) 1028
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 1 cut(s) 404
MspR9I CCNGG 1 cut(s) 564
MunI CAATTG 2 cut(s) 670, 810
MvaI CCWGG 1 cut(s) 564
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 3 cut(s) 137, 357, 1267
NcoI CCATGG 1 cut(s) 547
NdeII GATC 5 cut(s) 6, 939, 969, 989, 995
NlaIII CATG 8 cut(s) 351, 414, 551, 604, 869, 912, 981, 1033
NlaIV GGNNCC 1 cut(s) 544
NmuCI GTSAC 1 cut(s) 1187
NsiI ATGCAT 1 cut(s) 1176
NspI RCATGY 1 cut(s) 912
NspV TTCGAA 1 cut(s) 917
PaqCI CACCTGC 1 cut(s) 721
PdmI GAANNNNTTC 1 cut(s) 820
PfeI GAWTC 5 cut(s) 407, 456, 517, 633, 692
PflMI CCANNNNNTGG 2 cut(s) 398, 866
PkrI GCNGC 3 cut(s) 120, 139, 142
PleI GAGTC 3 cut(s) 621, 929, 1184
PpsI GAGTC 3 cut(s) 621, 929, 1184
Ppu21I YACGTR 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 542
PshAI GACNNNNGTC 1 cut(s) 672
PsiI TTATAA 1 cut(s) 828
Psp1406I AACGTT 1 cut(s) 681
Psp5II RGGWCCY 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 562
PspGI CCWGG 1 cut(s) 562
PspN4I GGNNCC 1 cut(s) 544
PspPI GGNCC 5 cut(s) 344, 401, 542, 566, 857
PspPPI RGGWCCY 1 cut(s) 542
PstI CTGCAG 2 cut(s) 372, 1108
PstNI CAGNNNCTG 1 cut(s) 998
PsuI RGATCY 1 cut(s) 995
RsaI GTAC 4 cut(s) 181, 1130, 1147, 1204
RsaNI GTAC 4 cut(s) 180, 1129, 1146, 1203
RseI CAYNNNNRTG 1 cut(s) 1028
SacII CCGCGG 1 cut(s) 138
SaqAI TTAA 2 cut(s) 249, 1378
SatI GCNGC 3 cut(s) 119, 138, 141
Sau3AI GATC 5 cut(s) 6, 939, 969, 989, 995
Sau96I GGNCC 5 cut(s) 344, 401, 542, 566, 857
SchI GAGTC 3 cut(s) 621, 929, 1184
ScrFI CCNGG 1 cut(s) 564
SduI GDGCHC 1 cut(s) 608
SetI ASST 9 cut(s) 571, 596, 684, 690, 715, 988, 1103, 1212, 1376
SfaNI GCATC 4 cut(s) 462, 1185, 1341, 1347
SfcI CTRYAG 3 cut(s) 368, 451, 1104
Sfr303I CCGCGG 1 cut(s) 138
SfuI TTCGAA 1 cut(s) 917
SgrBI CCGCGG 1 cut(s) 138
SinI GGWCC 4 cut(s) 401, 542, 566, 857
SmiMI CAYNNNNRTG 1 cut(s) 1028
SnaBI TACGTA 1 cut(s) 688
Sse9I AATT 9 cut(s) 108, 283, 670, 738, 810, 898, 1229, 1302, 1346
SsiI CCGC 5 cut(s) 119, 135, 137, 171, 1268
SspI AATATT 1 cut(s) 832
SspMI CTAG 1 cut(s) 1058
StyD4I CCNGG 1 cut(s) 562
StyI CCWWGG 1 cut(s) 547
TaaI ACNGT 8 cut(s) 164, 187, 266, 327, 438, 928, 1039, 1309
TaiI ACGT 2 cut(s) 684, 690
TaqI TCGA 5 cut(s) 470, 666, 917, 938, 1193
TasI AATT 9 cut(s) 108, 283, 670, 738, 810, 898, 1229, 1302, 1346
TatI WGTACW 2 cut(s) 1128, 1202
TauI GCSGC 2 cut(s) 121, 140
TfiI GAWTC 5 cut(s) 407, 456, 517, 633, 692
Tru1I TTAA 2 cut(s) 249, 1378
Tru9I TTAA 2 cut(s) 249, 1378
TscAI CASTG 5 cut(s) 120, 271, 443, 564, 1192
TseFI GTSAC 1 cut(s) 1187
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 1187
TspDTI ATGAA 4 cut(s) 399, 589, 644, 779
TspRI CASTG 5 cut(s) 120, 271, 443, 564, 1192
Van91I CCANNNNNTGG 2 cut(s) 398, 866
VpaK11BI GGWCC 4 cut(s) 401, 542, 566, 857
XapI RAATTY 3 cut(s) 108, 283, 738
XceI RCATGY 1 cut(s) 912
XmiI GTMKAC 1 cut(s) 329
XmnI GAANNNNTTC 1 cut(s) 820
XspI CTAG 1 cut(s) 1058
Zsp2I ATGCAT 1 cut(s) 1176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.