Rorug03G0024000

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0015816
Physical Location & Seq
Forward (+)
1904428 .. 1904814
387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0024000.1

Sequence Viewer

Length: 387 bp
ATGGAGCAACTTAGAAACCGCGAAAATCGTCAGCAGAGAGCTGAGAGGCAAAAGAGGAGCAAAGCTGAAACACCCATTAAGATCAAGTATATTTCCAGCCCTATGATGGTTCAAGCCAACAATGCTTCAGAGTTCAGAGCAATTGTTCAGGAACTCACCGGCCAAAATTCGGACACTACCATTCCAGATCATGGACCAAACCAAGCAAGCTGGTTTTCTAACCACAGAGCTTCACAATCAGCAAATATGAAAACCCAAAACGCTGATGAGCTGATGAAATTTTCAGATAACCGTGTAGAAGTTTTCAGTCAGTTTGATGAAATTAAGTTCTGGAGGGAAGTTTCAGAGAGCTTCTGCGCTTCCGAATCTCCATATGGTTATGTATGA

Protein Analysis

128

Amino Acids

14.87

Weight (kDa)

6.74

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 31 - 58 7.7e-09 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015816)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56710
fragaria_vesca FvH4_6g08130
malus_domestica MD04G1189600.v1.1
prunus_persica Prupe.6G307400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0457981
rosa_laevigata RLG00000025181
rosa_multiflora Rmu_sc0000384.1_g000005
rosa_roxburghii Rroxscaffold_6G00421410
rosa_rugosa Rorug03G0024000
rosa_samantha Rh3AG084600 Rh3BG087300 Rh3CG087800 Rh3DG088100
rosa_wichuraiana Rw0G012380 Rw3G007200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 191
AccII CGCG 1 cut(s) 21
AciI CCGC 1 cut(s) 19
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 2 cut(s) 166, 278
AcuI CTGAAG 1 cut(s) 111
AfiI CCNNNNNNNGG 3 cut(s) 106, 169, 191
AgsI TTSAA 1 cut(s) 113
AluBI AGCT 6 cut(s) 41, 65, 210, 230, 271, 351
AluI AGCT 6 cut(s) 41, 65, 210, 230, 271, 351
AoxI GGCC 1 cut(s) 160
ApoI RAATTY 2 cut(s) 166, 278
AspLEI GCGC 1 cut(s) 359
AspS9I GGNCC 1 cut(s) 194
AsuHPI GGTGA 1 cut(s) 148
AvaII GGWCC 1 cut(s) 194
BccI CCATC 1 cut(s) 100
Bme18I GGWCC 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 194
BpmI CTGGAG 1 cut(s) 352
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 169, 191
Bse118I RCCGGY 1 cut(s) 158
BseLI CCNNNNNNNGG 3 cut(s) 106, 169, 191
BseMII CTCAG 1 cut(s) 33
BseRI GAGGAG 1 cut(s) 70
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 1 cut(s) 162
BsiSI CCGG 1 cut(s) 159
BslI CCNNNNNNNGG 3 cut(s) 106, 169, 191
BsnI GGCC 1 cut(s) 162
Bsp143I GATC 2 cut(s) 81, 187
BspACI CCGC 1 cut(s) 19
BspANI GGCC 1 cut(s) 162
BspCNI CTCAG 1 cut(s) 34
BspFNI CGCG 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 158
BssMI GATC 2 cut(s) 81, 187
Bst4CI ACNGT 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 2 cut(s) 11, 42
BstFNI CGCG 1 cut(s) 21
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 2 cut(s) 84, 190
BstMBI GATC 2 cut(s) 81, 187
BstMWI GCNNNNNNNGC 1 cut(s) 122
BstUI CGCG 1 cut(s) 21
BsuRI GGCC 1 cut(s) 162
Cac8I GCNNGC 1 cut(s) 208
CfoI GCGC 1 cut(s) 359
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 194
CviAII CATG 1 cut(s) 191
CviJI RGCY 9 cut(s) 41, 65, 99, 116, 162, 210, 230, 271, 351
CviKI_1 RGCY 9 cut(s) 41, 65, 99, 116, 162, 210, 230, 271, 351
DdeI CTNAG 2 cut(s) 11, 42
DpnI GATC 2 cut(s) 83, 189
DpnII GATC 2 cut(s) 81, 187
EaeI YGGCCR 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 111
FaeI CATG 1 cut(s) 194
FaiI YATR 8 cut(s) 90, 104, 192, 248, 373, 375, 381, 385
FatI CATG 1 cut(s) 190
FauNDI CATATG 1 cut(s) 373
GlaI GCGC 1 cut(s) 358
GsuI CTGGAG 1 cut(s) 352
HaeIII GGCC 1 cut(s) 162
HapII CCGG 1 cut(s) 159
HhaI GCGC 1 cut(s) 359
Hin1II CATG 1 cut(s) 194
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HinfI GANTC 1 cut(s) 365
HpaII CCGG 1 cut(s) 159
HphI GGTGA 1 cut(s) 148
Hpy188I TCNGA 6 cut(s) 130, 137, 172, 286, 346, 364
Hpy188III TCNNGA 3 cut(s) 149, 185, 331
HpyCH4III ACNGT 1 cut(s) 293
HpyF10VI GCNNNNNNNGC 1 cut(s) 122
HpyF3I CTNAG 2 cut(s) 11, 42
Hsp92II CATG 1 cut(s) 194
HspAI GCGC 1 cut(s) 357
Kzo9I GATC 2 cut(s) 81, 187
LmnI GCTCC 2 cut(s) 4, 57
LpnPI CCDG 6 cut(s) 109, 134, 172, 196, 198, 316
MalI GATC 2 cut(s) 83, 189
MboI GATC 2 cut(s) 81, 187
MfeI CAATTG 1 cut(s) 141
MluCI AATT 4 cut(s) 141, 166, 278, 321
MnlI CCTC 3 cut(s) 39, 48, 327
MseI TTAA 2 cut(s) 78, 324
MspI CCGG 1 cut(s) 159
MunI CAATTG 1 cut(s) 141
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 1 cut(s) 122
NdeI CATATG 1 cut(s) 373
NdeII GATC 2 cut(s) 81, 187
NlaIII CATG 1 cut(s) 194
PfeI GAWTC 1 cut(s) 365
PflMI CCANNNNNTGG 1 cut(s) 191
PspPI GGNCC 1 cut(s) 194
SaqAI TTAA 2 cut(s) 78, 324
Sau3AI GATC 2 cut(s) 81, 187
Sau96I GGNCC 1 cut(s) 194
SetI ASST 6 cut(s) 43, 67, 212, 232, 273, 353
SinI GGWCC 1 cut(s) 194
Sse9I AATT 4 cut(s) 141, 166, 278, 321
SsiI CCGC 1 cut(s) 19
TaaI ACNGT 1 cut(s) 293
TasI AATT 4 cut(s) 141, 166, 278, 321
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 2 cut(s) 78, 324
Tru9I TTAA 2 cut(s) 78, 324
TspDTI ATGAA 3 cut(s) 263, 290, 333
Van91I CCANNNNNTGG 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 194
XapI RAATTY 2 cut(s) 166, 278
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.