Rw3G007200

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0015816
Physical Location & Seq
Reverse (-)
6646747 .. 6649077
2331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G007200.1

Sequence Viewer

Length: 1191 bp
ATGCTGATCAACTTTGTTCTTACTCAAATTTTCAACTTCTTTTTTTGTTTCTTATTTCTTCCAACACACGGCAGACTTCACTACACTTCTTCGCTTTCACTGTCTCAAATTTCAGTGCCTCCTTCTCCTGCACCCACGGCTGCCGTTATCCCGAGTTACAATGGTAGTTCTGCTCTTGGTACCACTGTTTTCAATGTGAGAACTTTTGGTGCAGTTGGGGATGGTGTTACTGATGATACAAAAGCATTTAAAATGGCTTGGGACAGTGCTTGTCAAACTGAAGATTCGGAAGTTCTTGTTCCAAAAGGGCATTCCTTTATGTTACAGTCTACAATATTCACAGGGCCGTGTAAATCTGGCCTCAAATTGCAGATTGATGGGACATTAGTGCCGCCAAATGGACCGGATTCATGGCCGAAAAATATTAGTAAGAGACAGTGGTTGGTTTTCTACAGAATCAATGGAATGTCAATGCAAGGAGGTGGTGTAATAGATGGTAGAGGAGAGAAATGGTGGAATCTTCCTTGCAAACCCCACAAAGGAATCAATGGAACAACAATGCCTGGACCTTGTGATAGCCCGGCTGCTGTAAGGTTCTTCATGTGCTCAAATCTGAAACTGCAAGGACTCAAAATCAAGAATAGCCCCCAGTTTCACTGCAAGTTTGATAATTGTCAAAACGTTTTCATAGAATCACTTACCATAAATTCACCTGCTCTAAGTCCCAACACAGACGGAATTCACATACAGAACACAAATAATGTGAAAATATACAATTCACTCGTATCCAATGGTGATGATTGTGTATCAATTGGGACTGGTTCTTATAATATGGAAATTAGGAATATTACTTGCGGTCCAAGTCATGGAATAAGCATGTGTTCGAATATCACAGTTAGAGACTCGATCATCAAGCACTCTGACAATGGGGTTCGGATCAAAACATGGCAAGGGGGATCAGGATCTGTGTCGAGCATAACATTCCACAACATTCACATGGACACCGTACGAAACCCCATTCCCGCCTATGCATTTCTGGAATTGCTCCCTGCTACAAAAGGACGCTTGGTGTCGAGCCCGCTTTGCTGGAGTGCTTATGGAACTATGCAGACAATTACCGTTCCACCAGTTTTGTGCTTGTTAGGGGGCATCCCGGAACTGTTGCCCCAGAATGATAATGATGGGTGTTAA

Protein Analysis

396

Amino Acids

42.99

Weight (kDa)

8.35

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 64 - 112 4.4e-07 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 94 - 340 4.3e-64 Glycosyl hydrolases family 28
Beta_helix PF13229 198 - 330 1e-05 Right handed beta helix region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015816)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56710
fragaria_vesca FvH4_6g08130
malus_domestica MD04G1189600.v1.1
prunus_persica Prupe.6G307400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0457981
rosa_laevigata RLG00000025181
rosa_multiflora Rmu_sc0000384.1_g000005
rosa_roxburghii Rroxscaffold_6G00421410
rosa_rugosa Rorug03G0024000
rosa_samantha Rh3AG084600 Rh3BG087300 Rh3CG087800 Rh3DG088100
rosa_wichuraiana Rw0G012380 Rw3G007200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 828
AarI CACCTGC 1 cut(s) 721
Acc36I ACCTGC 1 cut(s) 721
Acc65I GGTACC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 1 cut(s) 866
AccI GTMKAC 1 cut(s) 329
AciI CCGC 4 cut(s) 392, 855, 1023, 1079
AclI AACGTT 1 cut(s) 681
AclWI GGATC 3 cut(s) 944, 964, 970
AcoI YGGCCR 1 cut(s) 413
AcsI RAATTY 4 cut(s) 27, 108, 706, 738
AcuI CTGAAG 1 cut(s) 300
AfaI GTAC 2 cut(s) 181, 1008
AfiI CCNNNNNNNGG 4 cut(s) 68, 398, 539, 866
AgsI TTSAA 2 cut(s) 34, 193
AjnI CCWGG 1 cut(s) 562
AjuI GAANNNNNNNTTGG 2 cut(s) 295, 327
Alw21I GWGCWC 1 cut(s) 608
Alw26I GTCTC 3 cut(s) 108, 427, 894
AlwI GGATC 3 cut(s) 944, 964, 970
AlwNI CAGNNNCTG 1 cut(s) 965
Ama87I CYCGRG 1 cut(s) 151
AoxI GGCC 3 cut(s) 344, 358, 413
ApeKI GCWGC 2 cut(s) 140, 584
ApoI RAATTY 4 cut(s) 27, 108, 706, 738
Asp718I GGTACC 1 cut(s) 179
AspS9I GGNCC 4 cut(s) 344, 401, 566, 857
AsuC2I CCSGG 2 cut(s) 581, 1154
AsuHPI GGTGA 2 cut(s) 702, 806
AsuII TTCGAA 1 cut(s) 884
AvaI CYCGRG 1 cut(s) 151
AvaII GGWCC 3 cut(s) 401, 566, 857
BaeI ACNNNNGTAYC 2 cut(s) 171, 204
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 1 cut(s) 1079
Bbv12I GWGCWC 1 cut(s) 608
BbvI GCAGC 2 cut(s) 127, 571
BccI CCATC 4 cut(s) 215, 371, 488, 1175
BceAI ACGGC 4 cut(s) 85, 128, 153, 331
BciT130I CCWGG 1 cut(s) 564
BciVI GTATCC 1 cut(s) 796
BclI TGATCA 1 cut(s) 6
BcnI CCSGG 2 cut(s) 581, 1154
BcoDI GTCTC 3 cut(s) 108, 427, 894
BfmI CTRYAG 1 cut(s) 451
BfuAI ACCTGC 1 cut(s) 721
BfuI GTATCC 1 cut(s) 796
BisI GCNGC 3 cut(s) 141, 392, 585
BlsI GCNGC 3 cut(s) 142, 393, 586
Bme1390I CCNGG 3 cut(s) 564, 581, 1154
Bme18I GGWCC 3 cut(s) 401, 566, 857
BmeT110I CYCGRG 1 cut(s) 151
BmgT120I GGNCC 4 cut(s) 344, 401, 566, 857
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 3 cut(s) 564, 581, 1154
BmrI ACTGGG 1 cut(s) 643
BmsI GCATC 1 cut(s) 1158
BmuI ACTGGG 1 cut(s) 643
BpmI CTGGAG 1 cut(s) 1108
Bpu14I TTCGAA 1 cut(s) 884
BpuMI CCSGG 2 cut(s) 581, 1154
BsaBI GATNNNNATC 1 cut(s) 961
BsaJI CCNNGG 1 cut(s) 135
BsaWI WCCGGW 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 4 cut(s) 68, 398, 539, 866
Bse1I ACTGG 3 cut(s) 649, 823, 1127
Bse8I GATNNNNATC 1 cut(s) 961
BseBI CCWGG 1 cut(s) 564
BseDI CCNNGG 1 cut(s) 135
BseGI GGATG 2 cut(s) 226, 1149
BseJI GATNNNNATC 1 cut(s) 961
BseLI CCNNNNNNNGG 4 cut(s) 68, 398, 539, 866
BseNI ACTGG 3 cut(s) 649, 823, 1127
BseRI GAGGAG 1 cut(s) 516
BseXI GCAGC 2 cut(s) 127, 571
BsgI GTGCAG 2 cut(s) 114, 231
BshFI GGCC 3 cut(s) 346, 360, 415
BshNI GGYRCC 1 cut(s) 179
BsiHKAI GWGCWC 1 cut(s) 608
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 3 cut(s) 404, 581, 1154
BsiWI CGTACG 1 cut(s) 1006
BslFI GGGAC 4 cut(s) 275, 394, 708, 829
BslI CCNNNNNNNGG 4 cut(s) 68, 398, 539, 866
BsmAI GTCTC 3 cut(s) 108, 427, 894
BsmFI GGGAC 4 cut(s) 275, 394, 708, 829
BsmI GAATGC 1 cut(s) 310
BsnI GGCC 3 cut(s) 346, 360, 415
BsoBI CYCGRG 1 cut(s) 151
Bsp119I TTCGAA 1 cut(s) 884
Bsp1286I GDGCHC 2 cut(s) 608, 1079
Bsp143I GATC 5 cut(s) 6, 906, 936, 956, 962
BspACI CCGC 4 cut(s) 392, 855, 1023, 1079
BspANI GGCC 3 cut(s) 346, 360, 415
BspLI GGNNCC 1 cut(s) 181
BspMI ACCTGC 1 cut(s) 721
BspPI GGATC 3 cut(s) 944, 964, 970
BspT104I TTCGAA 1 cut(s) 884
BspT107I GGYRCC 1 cut(s) 179
BsrI ACTGG 3 cut(s) 649, 823, 1127
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 5 cut(s) 6, 906, 936, 956, 962
Bst2UI CCWGG 1 cut(s) 564
Bst4CI ACNGT 9 cut(s) 102, 187, 266, 327, 438, 895, 1006, 1120, 1161
BstBI TTCGAA 1 cut(s) 884
BstC8I GCNNGC 1 cut(s) 1079
BstDEI CTNAG 1 cut(s) 719
BstDSI CCRYGG 1 cut(s) 135
BstF5I GGATG 2 cut(s) 226, 1149
BstKTI GATC 5 cut(s) 9, 909, 939, 959, 965
BstMAI GTCTC 3 cut(s) 108, 427, 894
BstMBI GATC 5 cut(s) 6, 906, 936, 956, 962
BstMWI GCNNNNNNNGC 2 cut(s) 137, 1083
BstNI CCWGG 1 cut(s) 564
BstNSI RCATGY 1 cut(s) 880
BstSCI CCNGG 3 cut(s) 562, 579, 1152
BstSFI CTRYAG 1 cut(s) 451
BstV1I GCAGC 2 cut(s) 127, 571
BstX2I RGATCY 1 cut(s) 962
BstYI RGATCY 1 cut(s) 962
BsuI GTATCC 1 cut(s) 796
BsuRI GGCC 3 cut(s) 346, 360, 415
BtgI CCRYGG 1 cut(s) 135
BtsCI GGATG 2 cut(s) 226, 1149
BtsI GCAGTG 1 cut(s) 655
BtsIMutI CAGTG 6 cut(s) 98, 120, 183, 271, 443, 655
BveI ACCTGC 1 cut(s) 721
Cac8I GCNNGC 1 cut(s) 1079
CaiI CAGNNNCTG 1 cut(s) 965
Cfr13I GGNCC 4 cut(s) 344, 401, 566, 857
CseI GACGC 1 cut(s) 1071
Csp6I GTAC 2 cut(s) 180, 1007
CspCI CAANNNNNGTGG 2 cut(s) 1113, 1148
CviAII CATG 6 cut(s) 411, 601, 866, 877, 945, 997
CviJI RGCY 9 cut(s) 140, 257, 346, 360, 415, 579, 584, 645, 1077
CviKI_1 RGCY 9 cut(s) 140, 257, 346, 360, 415, 579, 584, 645, 1077
CviQI GTAC 2 cut(s) 180, 1007
DdeI CTNAG 1 cut(s) 719
DpnI GATC 5 cut(s) 8, 908, 938, 958, 964
DpnII GATC 5 cut(s) 6, 906, 936, 956, 962
DraI TTTAAA 1 cut(s) 250
EaeI YGGCCR 1 cut(s) 413
Eco24I GRGCYC 1 cut(s) 1079
Eco47I GGWCC 3 cut(s) 401, 566, 857
Eco57I CTGAAG 1 cut(s) 300
Eco88I CYCGRG 1 cut(s) 151
EcoRI GAATTC 1 cut(s) 738
EcoRII CCWGG 1 cut(s) 562
EcoT22I ATGCAT 1 cut(s) 1033
EcoT38I GRGCYC 1 cut(s) 1079
FaeI CATG 6 cut(s) 414, 604, 869, 880, 948, 1000
FaqI GGGAC 4 cut(s) 275, 394, 708, 829
FatI CATG 6 cut(s) 410, 600, 865, 876, 944, 996
FauI CCCGC 2 cut(s) 1030, 1086
FbaI TGATCA 1 cut(s) 6
FblI GTMKAC 1 cut(s) 329
Fnu4HI GCNGC 3 cut(s) 141, 392, 585
FokI GGATG 2 cut(s) 233, 1136
FriOI GRGCYC 1 cut(s) 1079
Fsp4HI GCNGC 3 cut(s) 141, 392, 585
GluI GCNGC 3 cut(s) 141, 392, 585
GsuI CTGGAG 1 cut(s) 1108
HaeIII GGCC 3 cut(s) 346, 360, 415
HapII CCGG 3 cut(s) 404, 581, 1154
HgaI GACGC 1 cut(s) 1071
Hin1II CATG 6 cut(s) 414, 604, 869, 880, 948, 1000
HinfI GANTC 8 cut(s) 284, 407, 456, 517, 543, 627, 692, 902
HpaII CCGG 3 cut(s) 404, 581, 1154
HphI GGTGA 2 cut(s) 702, 806
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 4 cut(s) 289, 615, 922, 936
Hpy188III TCNNGA 4 cut(s) 151, 637, 960, 1037
Hpy8I GTNNAC 1 cut(s) 330
HpyAV CCTTC 1 cut(s) 132
HpyCH4III ACNGT 9 cut(s) 102, 187, 266, 327, 438, 895, 1006, 1120, 1161
HpyCH4IV ACGT 1 cut(s) 681
HpyCH4V TGCA 9 cut(s) 131, 212, 370, 475, 528, 622, 660, 1031, 1108
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 1083
HpyF3I CTNAG 1 cut(s) 719
HpySE526I ACGT 1 cut(s) 681
Hsp92II CATG 6 cut(s) 414, 604, 869, 880, 948, 1000
KpnI GGTACC 1 cut(s) 183
Ksp22I TGATCA 1 cut(s) 6
Kzo9I GATC 5 cut(s) 6, 906, 936, 956, 962
LmnI GCTCC 1 cut(s) 1050
Lsp1109I GCAGC 2 cut(s) 127, 571
LweI GCATC 1 cut(s) 1158
MaeII ACGT 1 cut(s) 681
MaeIII GTNAC 3 cut(s) 155, 226, 321
MalI GATC 5 cut(s) 8, 908, 938, 958, 964
MboI GATC 5 cut(s) 6, 906, 936, 956, 962
MboII GAAGA 5 cut(s) 50, 81, 293, 512, 589
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 1 cut(s) 962
MhlI GDGCHC 2 cut(s) 608, 1079
MlyI GAGTC 2 cut(s) 621, 896
MmeI TCCRAC 1 cut(s) 86
MnlI CCTC 4 cut(s) 129, 371, 473, 494
Mph1103I ATGCAT 1 cut(s) 1033
MseI TTAA 2 cut(s) 249, 1189
MslI CAYNNNNRTG 1 cut(s) 995
MspI CCGG 3 cut(s) 404, 581, 1154
MspR9I CCNGG 3 cut(s) 564, 581, 1154
MunI CAATTG 1 cut(s) 810
Mva1269I GAATGC 1 cut(s) 310
MvaI CCWGG 1 cut(s) 564
MwoI GCNNNNNNNGC 2 cut(s) 137, 1083
NciI CCSGG 2 cut(s) 581, 1154
NdeII GATC 5 cut(s) 6, 906, 936, 956, 962
NlaIII CATG 6 cut(s) 414, 604, 869, 880, 948, 1000
NlaIV GGNNCC 1 cut(s) 181
NsiI ATGCAT 1 cut(s) 1033
NspI RCATGY 1 cut(s) 880
NspV TTCGAA 1 cut(s) 884
PaqCI CACCTGC 1 cut(s) 721
PctI GAATGC 1 cut(s) 310
PfeI GAWTC 6 cut(s) 284, 407, 456, 517, 543, 692
Pfl23II CGTACG 1 cut(s) 1006
PflMI CCANNNNNTGG 1 cut(s) 866
PfoI TCCNGGA 1 cut(s) 1152
PkrI GCNGC 3 cut(s) 142, 393, 586
PleI GAGTC 2 cut(s) 621, 896
PpsI GAGTC 2 cut(s) 621, 896
PsiI TTATAA 1 cut(s) 828
Psp1406I AACGTT 1 cut(s) 681
Psp6I CCWGG 1 cut(s) 562
PspGI CCWGG 1 cut(s) 562
PspLI CGTACG 1 cut(s) 1006
PspN4I GGNNCC 1 cut(s) 181
PspPI GGNCC 4 cut(s) 344, 401, 566, 857
PstNI CAGNNNCTG 1 cut(s) 965
PsuI RGATCY 1 cut(s) 962
RsaI GTAC 2 cut(s) 181, 1008
RsaNI GTAC 2 cut(s) 180, 1007
RseI CAYNNNNRTG 1 cut(s) 995
SaqAI TTAA 2 cut(s) 249, 1189
SatI GCNGC 3 cut(s) 141, 392, 585
Sau3AI GATC 5 cut(s) 6, 906, 936, 956, 962
Sau96I GGNCC 4 cut(s) 344, 401, 566, 857
SchI GAGTC 2 cut(s) 621, 896
ScrFI CCNGG 3 cut(s) 564, 581, 1154
SduI GDGCHC 2 cut(s) 608, 1079
SetI ASST 5 cut(s) 484, 571, 596, 684, 715
SfaNI GCATC 1 cut(s) 1158
SfcI CTRYAG 1 cut(s) 451
SfuI TTCGAA 1 cut(s) 884
SinI GGWCC 3 cut(s) 401, 566, 857
SmiMI CAYNNNNRTG 1 cut(s) 995
SsiI CCGC 4 cut(s) 392, 855, 1023, 1079
SspI AATATT 3 cut(s) 336, 424, 847
StyD4I CCNGG 3 cut(s) 562, 579, 1152
TaaI ACNGT 9 cut(s) 102, 187, 266, 327, 438, 895, 1006, 1120, 1161
TaiI ACGT 1 cut(s) 684
TaqI TCGA 4 cut(s) 884, 905, 971, 1073
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 6 cut(s) 284, 407, 456, 517, 543, 692
Tru1I TTAA 2 cut(s) 249, 1189
Tru9I TTAA 2 cut(s) 249, 1189
TscAI CASTG 6 cut(s) 105, 120, 190, 271, 443, 662
TseI GCWGC 2 cut(s) 140, 584
TspDTI ATGAA 3 cut(s) 399, 589, 676
TspGWI ACGGA 1 cut(s) 750
TspRI CASTG 6 cut(s) 105, 120, 190, 271, 443, 662
Van91I CCANNNNNTGG 1 cut(s) 866
VpaK11BI GGWCC 3 cut(s) 401, 566, 857
XapI RAATTY 4 cut(s) 27, 108, 706, 738
XceI RCATGY 1 cut(s) 880
XmiI GTMKAC 1 cut(s) 329
Zsp2I ATGCAT 1 cut(s) 1033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.