RLG00000025181

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0015816
Physical Location & Seq
Forward (+)
42847986 .. 42850874
2889 bp
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UTR
Exon/CDS
Intron
RLM00000025181

Sequence Viewer

Length: 1479 bp
ATGCCAGTGCTCGCATGCATTCTCTCAGCAAAAGAAGCCAAAGCCATAGCTCTAAATGGAGGTGGTCGGTTTCTCAACACTGTTGCATGTGTTCCCTCTGATATTCCCAAAGTTTGTAGTAGGGTTTATCTCTCTGCAACTTGTCTGGTGGTGGTGGTGATCAAAGTGGTCACAGTTCACTACACTTCTTCGCTTTCACTGTCTCAAATTTCAGTGCCTCCTTCTCCTGCACCCACGGCTGCTGTTATCCCGAGTTACAATGGTAGTTCTGCTCTTGGTACCACTGTTTTCAATGTGAGAACTTTTGGTGCAGTTGGGGATGGTGTTACTGATGATACAGAAGCATTTAAAATGGCTTGGGACAGTGCTTGTCAAACTGAAGATTCGGAAGTTCTTGTTCCAAAAGGGCATTCCTTTATGTTACAGTCTACAATATTCACGGGGCCGTGTAAATCTGGCCTCAAATTGCAGATTGTTGGGACATTAGTGCCGCCAAATGGACCGGATTCATGGCCGAAAAATATTAGTAAGAGACAGTGGTTGGTTTTCTACAGAATCAATGGAATGTCAATGCAAGGAGGTGGTGTAATAGATGGTAGAGGAGAGAAATGGTGGAATCTTCCTTGCAAACCCCACAAAGGAATCAATGGAACAACAATGCCTGGACCTTGTGATAGCCCGGCTGCTGTAAGGTTCTTCATGTGCTCAAATCTGAAACTTCAAGGACTCAAAATCAAGAATAGCCCCCAGTTTCACTTCAAGTTTGATAACTGTCAGAACGTTCTCATAGAATCACTTACCATAAATTCACCTGCTCTAAGTCCCAACACAGACGGAATTCACATACAGAACACAAATAATGTGAAAATATACAATTCACTCGTGTCCAATGGTGATGATTGTGTATCAATTGGGACTGGTTCTTATAATATAGAAATTAGGAATATTACTTGTGGTCCAAGTCATGGAATAAGCATTGGCAGTCTTGGAATTGGCAATTCCCGAGCATGTGTTTCAAATATCACAGTTAGAGACTCGATCATCAAGCACTCTGACAATGGGGTTCGGATCAAAACATGGCAAGGGGGATCAGGATCTGTGTCAAGCATAACATTCCACAACATTCACATGGACAACGTACGAAACCCCATTGTGCTAGACCAATATTACTGCCTCACCAAGCATTGCCCTAACCAAACCTCCGCAGTTCACATATCAAATATCTTGTACACCAACATAAGGGGTACTTATGATGTGCGAAGCCCGCCTATGCATTTCGCTTGCAGTGACTCAATGCCTTGCACAAACCTCACTCTCTCTGAAGTGGAATTGCTCCCTGCTACAAAAGGACGCTTGGTGTCGAGCCCGCTTTGCTGGAGTGCTTATGGAACTATGCAGACAATTACCGTTCCACCAGTTTTGTGCTTGTTAGGGGGCATCCCAGAACTGTTGCCCCAGAATGATAATGATGGGTGTTAA

Protein Analysis

493

Amino Acids

52.76

Weight (kDa)

8.43

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 97 - 145 3.5e-07 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 127 - 444 3.4e-93 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015816)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56710
fragaria_vesca FvH4_6g08130
malus_domestica MD04G1189600.v1.1
prunus_persica Prupe.6G307400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0457981
rosa_laevigata RLG00000025181
rosa_multiflora Rmu_sc0000384.1_g000005
rosa_roxburghii Rroxscaffold_6G00421410
rosa_rugosa Rorug03G0024000
rosa_samantha Rh3AG084600 Rh3BG087300 Rh3CG087800 Rh3DG088100
rosa_wichuraiana Rw0G012380 Rw3G007200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 927
AarI CACCTGC 1 cut(s) 820
Acc36I ACCTGC 1 cut(s) 820
Acc65I GGTACC 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 278
AccB7I CCANNNNNTGG 1 cut(s) 965
AccI GTMKAC 1 cut(s) 428
AciI CCGC 4 cut(s) 491, 1203, 1265, 1367
AclI AACGTT 1 cut(s) 780
AclWI GGATC 3 cut(s) 1076, 1096, 1102
AcoI YGGCCR 1 cut(s) 512
AcsI RAATTY 3 cut(s) 207, 805, 837
AcuI CTGAAG 2 cut(s) 399, 1341
AfaI GTAC 4 cut(s) 280, 1140, 1229, 1246
AfiI CCNNNNNNNGG 4 cut(s) 497, 638, 965, 1239
AgsI TTSAA 4 cut(s) 292, 722, 760, 1017
AjnI CCWGG 1 cut(s) 661
AjuI GAANNNNNNNTTGG 2 cut(s) 394, 426
AluBI AGCT 1 cut(s) 50
AluI AGCT 1 cut(s) 50
Alw21I GWGCWC 2 cut(s) 12, 707
Alw26I GTCTC 3 cut(s) 207, 526, 1026
AlwI GGATC 3 cut(s) 1076, 1096, 1102
AlwNI CAGNNNCTG 1 cut(s) 1097
Ama87I CYCGRG 2 cut(s) 250, 1002
AoxI GGCC 3 cut(s) 443, 457, 512
ApeKI GCWGC 2 cut(s) 239, 683
ApoI RAATTY 3 cut(s) 207, 805, 837
Asp718I GGTACC 1 cut(s) 278
AspS9I GGNCC 4 cut(s) 443, 500, 665, 956
AsuC2I CCSGG 1 cut(s) 680
AsuHPI GGTGA 4 cut(s) 169, 801, 905, 1168
AvaI CYCGRG 2 cut(s) 250, 1002
AvaII GGWCC 3 cut(s) 500, 665, 956
BaeI ACNNNNGTAYC 2 cut(s) 270, 303
BanI GGYRCC 1 cut(s) 278
BanII GRGCYC 1 cut(s) 1367
BauI CACGAG 1 cut(s) 881
Bbv12I GWGCWC 2 cut(s) 12, 707
BbvI GCAGC 2 cut(s) 226, 670
BccI CCATC 3 cut(s) 314, 587, 1463
BceAI ACGGC 2 cut(s) 252, 430
BciT130I CCWGG 1 cut(s) 663
BclI TGATCA 1 cut(s) 159
BcnI CCSGG 1 cut(s) 680
BcoDI GTCTC 3 cut(s) 207, 526, 1026
BfaI CTAG 1 cut(s) 1157
BfmI CTRYAG 1 cut(s) 550
BfuAI ACCTGC 1 cut(s) 820
BisI GCNGC 3 cut(s) 240, 491, 684
BlsI GCNGC 3 cut(s) 241, 492, 685
Bme1390I CCNGG 2 cut(s) 663, 680
Bme18I GGWCC 3 cut(s) 500, 665, 956
BmeT110I CYCGRG 2 cut(s) 250, 1002
BmgT120I GGNCC 4 cut(s) 443, 500, 665, 956
BmiI GGNNCC 2 cut(s) 280, 444
BmrFI CCNGG 2 cut(s) 663, 680
BmrI ACTGGG 1 cut(s) 742
BmsI GCATC 1 cut(s) 1446
BmuI ACTGGG 1 cut(s) 742
BpmI CTGGAG 1 cut(s) 1396
BpuMI CCSGG 1 cut(s) 680
BsaBI GATNNNNATC 1 cut(s) 1093
BsaJI CCNNGG 1 cut(s) 234
BsaWI WCCGGW 1 cut(s) 502
BsaXI ACNNNNNCTCC 4 cut(s) 570, 600, 1184, 1214
Bsc4I CCNNNNNNNGG 4 cut(s) 497, 638, 965, 1239
Bse1I ACTGG 4 cut(s) 5, 748, 922, 1415
Bse3DI GCAATG 1 cut(s) 1183
Bse8I GATNNNNATC 1 cut(s) 1093
BseBI CCWGG 1 cut(s) 663
BseDI CCNNGG 1 cut(s) 234
BseGI GGATG 2 cut(s) 325, 1437
BseJI GATNNNNATC 1 cut(s) 1093
BseLI CCNNNNNNNGG 4 cut(s) 497, 638, 965, 1239
BseMI GCAATG 1 cut(s) 1183
BseMII CTCAG 1 cut(s) 39
BseNI ACTGG 4 cut(s) 5, 748, 922, 1415
BseRI GAGGAG 1 cut(s) 615
BseXI GCAGC 2 cut(s) 226, 670
BsgI GTGCAG 2 cut(s) 213, 330
BshFI GGCC 3 cut(s) 445, 459, 514
BshNI GGYRCC 1 cut(s) 278
BsiHKAI GWGCWC 2 cut(s) 12, 707
BsiHKCI CYCGRG 2 cut(s) 250, 1002
BsiSI CCGG 2 cut(s) 503, 680
BsiWI CGTACG 1 cut(s) 1138
BslFI GGGAC 4 cut(s) 374, 493, 807, 928
BslI CCNNNNNNNGG 4 cut(s) 497, 638, 965, 1239
BsmAI GTCTC 3 cut(s) 207, 526, 1026
BsmFI GGGAC 4 cut(s) 374, 493, 807, 928
BsmI GAATGC 2 cut(s) 18, 409
BsnI GGCC 3 cut(s) 445, 459, 514
BsoBI CYCGRG 2 cut(s) 250, 1002
Bsp1286I GDGCHC 3 cut(s) 12, 707, 1367
Bsp1407I TGTACA 1 cut(s) 1227
Bsp143I GATC 5 cut(s) 159, 1038, 1068, 1088, 1094
BspACI CCGC 4 cut(s) 491, 1203, 1265, 1367
BspANI GGCC 3 cut(s) 445, 459, 514
BspCNI CTCAG 1 cut(s) 38
BspLI GGNNCC 2 cut(s) 280, 444
BspMI ACCTGC 1 cut(s) 820
BspPI GGATC 3 cut(s) 1076, 1096, 1102
BspT107I GGYRCC 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 1183
BsrGI TGTACA 1 cut(s) 1227
BsrI ACTGG 4 cut(s) 5, 748, 922, 1415
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 5 cut(s) 159, 1038, 1068, 1088, 1094
BssSI CACGAG 1 cut(s) 881
Bst2BI CACGAG 1 cut(s) 881
Bst2UI CCWGG 1 cut(s) 663
BstAUI TGTACA 1 cut(s) 1227
BstC8I GCNNGC 5 cut(s) 12, 16, 1265, 1282, 1367
BstDEI CTNAG 2 cut(s) 25, 818
BstDSI CCRYGG 1 cut(s) 234
BstF5I GGATG 2 cut(s) 325, 1437
BstKTI GATC 5 cut(s) 162, 1041, 1071, 1091, 1097
BstMAI GTCTC 3 cut(s) 207, 526, 1026
BstMBI GATC 5 cut(s) 159, 1038, 1068, 1088, 1094
BstMWI GCNNNNNNNGC 4 cut(s) 35, 236, 1264, 1371
BstNI CCWGG 1 cut(s) 663
BstNSI RCATGY 3 cut(s) 18, 90, 1011
BstSCI CCNGG 2 cut(s) 661, 678
BstSFI CTRYAG 1 cut(s) 550
BstV1I GCAGC 2 cut(s) 226, 670
BstX2I RGATCY 1 cut(s) 1094
BstYI RGATCY 1 cut(s) 1094
BsuRI GGCC 3 cut(s) 445, 459, 514
BtgI CCRYGG 1 cut(s) 234
BtsCI GGATG 2 cut(s) 325, 1437
BtsI GCAGTG 1 cut(s) 1291
BtsIMutI CAGTG 8 cut(s) 12, 78, 197, 219, 282, 370, 542, 1291
BveI ACCTGC 1 cut(s) 820
Cac8I GCNNGC 5 cut(s) 12, 16, 1265, 1282, 1367
CaiI CAGNNNCTG 1 cut(s) 1097
Cfr13I GGNCC 4 cut(s) 443, 500, 665, 956
CseI GACGC 1 cut(s) 1359
Csp6I GTAC 4 cut(s) 279, 1139, 1228, 1245
CspCI CAANNNNNGTGG 2 cut(s) 1401, 1436
CviAII CATG 8 cut(s) 15, 87, 510, 700, 965, 1008, 1077, 1129
CviQI GTAC 4 cut(s) 279, 1139, 1228, 1245
DdeI CTNAG 2 cut(s) 25, 818
DpnI GATC 5 cut(s) 161, 1040, 1070, 1090, 1096
DpnII GATC 5 cut(s) 159, 1038, 1068, 1088, 1094
DraI TTTAAA 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 512
Eco24I GRGCYC 1 cut(s) 1367
Eco47I GGWCC 3 cut(s) 500, 665, 956
Eco57I CTGAAG 2 cut(s) 399, 1341
Eco88I CYCGRG 2 cut(s) 250, 1002
EcoRI GAATTC 1 cut(s) 837
EcoRII CCWGG 1 cut(s) 661
EcoT22I ATGCAT 2 cut(s) 20, 1275
EcoT38I GRGCYC 1 cut(s) 1367
FaeI CATG 8 cut(s) 18, 90, 513, 703, 968, 1011, 1080, 1132
FalI AAGNNNNNCTT 2 cut(s) 1231, 1263
FaqI GGGAC 4 cut(s) 374, 493, 807, 928
FatI CATG 8 cut(s) 14, 86, 509, 699, 964, 1007, 1076, 1128
FauI CCCGC 2 cut(s) 1272, 1374
FbaI TGATCA 1 cut(s) 159
FblI GTMKAC 1 cut(s) 428
Fnu4HI GCNGC 3 cut(s) 240, 491, 684
FokI GGATG 2 cut(s) 332, 1424
FriOI GRGCYC 1 cut(s) 1367
Fsp4HI GCNGC 3 cut(s) 240, 491, 684
FspBI CTAG 1 cut(s) 1157
GluI GCNGC 3 cut(s) 240, 491, 684
GsuI CTGGAG 1 cut(s) 1396
HaeIII GGCC 3 cut(s) 445, 459, 514
HapII CCGG 2 cut(s) 503, 680
HgaI GACGC 1 cut(s) 1359
Hin1II CATG 8 cut(s) 18, 90, 513, 703, 968, 1011, 1080, 1132
HinfI GANTC 9 cut(s) 383, 506, 555, 616, 642, 726, 791, 1034, 1289
HpaII CCGG 2 cut(s) 503, 680
HphI GGTGA 4 cut(s) 169, 801, 905, 1168
Hpy166II GTNNAC 4 cut(s) 178, 429, 1210, 1230
Hpy188I TCNGA 7 cut(s) 100, 388, 714, 777, 1054, 1068, 1321
Hpy188III TCNNGA 4 cut(s) 250, 736, 1002, 1092
Hpy8I GTNNAC 4 cut(s) 178, 429, 1210, 1230
HpyAV CCTTC 1 cut(s) 231
HpyCH4IV ACGT 2 cut(s) 780, 1137
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 236, 1264, 1371
HpyF3I CTNAG 2 cut(s) 25, 818
HpySE526I ACGT 2 cut(s) 780, 1137
Hsp92II CATG 8 cut(s) 18, 90, 513, 703, 968, 1011, 1080, 1132
KpnI GGTACC 1 cut(s) 282
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 5 cut(s) 159, 1038, 1068, 1088, 1094
LmnI GCTCC 1 cut(s) 1338
Lsp1109I GCAGC 2 cut(s) 226, 670
LweI GCATC 1 cut(s) 1446
MaeI CTAG 1 cut(s) 1157
MaeII ACGT 2 cut(s) 780, 1137
MaeIII GTNAC 5 cut(s) 169, 254, 325, 420, 1286
MalI GATC 5 cut(s) 161, 1040, 1070, 1090, 1096
MboI GATC 5 cut(s) 159, 1038, 1068, 1088, 1094
MboII GAAGA 4 cut(s) 180, 392, 611, 688
MfeI CAATTG 1 cut(s) 909
MflI RGATCY 1 cut(s) 1094
MhlI GDGCHC 3 cut(s) 12, 707, 1367
MlyI GAGTC 3 cut(s) 720, 1028, 1283
MnlI CCTC 9 cut(s) 53, 106, 228, 470, 572, 593, 1184, 1210, 1319
Mph1103I ATGCAT 2 cut(s) 20, 1275
MseI TTAA 2 cut(s) 348, 1477
MslI CAYNNNNRTG 1 cut(s) 1127
MspI CCGG 2 cut(s) 503, 680
MspR9I CCNGG 2 cut(s) 663, 680
MunI CAATTG 1 cut(s) 909
Mva1269I GAATGC 2 cut(s) 18, 409
MvaI CCWGG 1 cut(s) 663
MwoI GCNNNNNNNGC 4 cut(s) 35, 236, 1264, 1371
NciI CCSGG 1 cut(s) 680
NdeII GATC 5 cut(s) 159, 1038, 1068, 1088, 1094
NlaIII CATG 8 cut(s) 18, 90, 513, 703, 968, 1011, 1080, 1132
NlaIV GGNNCC 2 cut(s) 280, 444
NmuCI GTSAC 2 cut(s) 169, 1286
NsiI ATGCAT 2 cut(s) 20, 1275
NspI RCATGY 3 cut(s) 18, 90, 1011
PaeI GCATGC 1 cut(s) 18
PaqCI CACCTGC 1 cut(s) 820
PctI GAATGC 2 cut(s) 18, 409
PfeI GAWTC 6 cut(s) 383, 506, 555, 616, 642, 791
Pfl23II CGTACG 1 cut(s) 1138
PflMI CCANNNNNTGG 1 cut(s) 965
PkrI GCNGC 3 cut(s) 241, 492, 685
PleI GAGTC 3 cut(s) 720, 1028, 1283
PpsI GAGTC 3 cut(s) 720, 1028, 1283
PsiI TTATAA 1 cut(s) 927
Psp1406I AACGTT 1 cut(s) 780
Psp6I CCWGG 1 cut(s) 661
PspGI CCWGG 1 cut(s) 661
PspLI CGTACG 1 cut(s) 1138
PspN4I GGNNCC 2 cut(s) 280, 444
PspPI GGNCC 4 cut(s) 443, 500, 665, 956
PstNI CAGNNNCTG 1 cut(s) 1097
PsuI RGATCY 1 cut(s) 1094
RsaI GTAC 4 cut(s) 280, 1140, 1229, 1246
RsaNI GTAC 4 cut(s) 279, 1139, 1228, 1245
RseI CAYNNNNRTG 1 cut(s) 1127
SaqAI TTAA 2 cut(s) 348, 1477
SatI GCNGC 3 cut(s) 240, 491, 684
Sau3AI GATC 5 cut(s) 159, 1038, 1068, 1088, 1094
Sau96I GGNCC 4 cut(s) 443, 500, 665, 956
SchI GAGTC 3 cut(s) 720, 1028, 1283
ScrFI CCNGG 2 cut(s) 663, 680
SduI GDGCHC 3 cut(s) 12, 707, 1367
SfaNI GCATC 1 cut(s) 1446
SfcI CTRYAG 1 cut(s) 550
SinI GGWCC 3 cut(s) 500, 665, 956
SmiMI CAYNNNNRTG 1 cut(s) 1127
SphI GCATGC 1 cut(s) 18
SsiI CCGC 4 cut(s) 491, 1203, 1265, 1367
SspI AATATT 4 cut(s) 435, 523, 946, 1166
SspMI CTAG 1 cut(s) 1157
StyD4I CCNGG 2 cut(s) 661, 678
TaiI ACGT 2 cut(s) 783, 1140
TaqI TCGA 2 cut(s) 1037, 1361
TatI WGTACW 1 cut(s) 1227
TauI GCSGC 1 cut(s) 493
TfiI GAWTC 6 cut(s) 383, 506, 555, 616, 642, 791
Tru1I TTAA 2 cut(s) 348, 1477
Tru9I TTAA 2 cut(s) 348, 1477
TscAI CASTG 8 cut(s) 12, 85, 204, 219, 289, 370, 542, 1291
TseFI GTSAC 2 cut(s) 169, 1286
TseI GCWGC 2 cut(s) 239, 683
Tsp45I GTSAC 2 cut(s) 169, 1286
TspDTI ATGAA 2 cut(s) 498, 688
TspGWI ACGGA 1 cut(s) 849
TspRI CASTG 8 cut(s) 12, 85, 204, 219, 289, 370, 542, 1291
Van91I CCANNNNNTGG 1 cut(s) 965
VpaK11BI GGWCC 3 cut(s) 500, 665, 956
XapI RAATTY 3 cut(s) 207, 805, 837
XceI RCATGY 3 cut(s) 18, 90, 1011
XmiI GTMKAC 1 cut(s) 428
XspI CTAG 1 cut(s) 1157
Zsp2I ATGCAT 2 cut(s) 20, 1275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.