FvH4_6g09700

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009696
Physical Location & Seq
Forward (+)
5773097 .. 5780135
7039 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g09700.t2

Sequence Viewer

Length: 3777 bp
ATGAGCGGTGATCATCTGTTGCTCTCATCACCGGATTCTCCATCAGCGCCGCTTGTGCAGCCCTCTACTCTAGCCGAGAGACTCGAGCAGCTCTTGCCTGATGGTTCCAGTTCTCCTTCCATTCTTGATAGCAACGATGATGGTCAAGCTGAATTGTTGTTGGTGAGGGAATACATTGCTGATTCTGACTGTGGTGAGAGACCGGGGAAGCAGATTTTTCCGGTAGGGCCGGAGTCTCGGCCGCAGTTCCCTTTGGAATACCCCACGCGGCATAAAAAACGCCAGGCGTCGTGGGGTACAATGGAACTGCACAGTATCAATGGTAATTCAGCGTCCCATGATGTTCTCCAGGCTCCATCTGGGGTTCAGGAAAAGGCAAACAAGTGTCACCCGGATATCCTCCTACATGAAGACCCAAGGTTGATCTATATTGATGATCCGAAGAGAACAAACGACAAGAATGAGTTCACTGGCAATGAGATTCGCACTAGCCAGTACACCTTAATCACATTTTTGCCCAAGAATGTTTTCATTCAGTTTCACCGGGTTGCTTATTTGTATTTTCTAGTTATTGCTGTTCTCAATCAGCTTCCGCCTCTTGCTGTCTTTGGGAGAACGGCATCTCTTTTCCCCCTCCTGTTTGTGCTATGTGTCACGGCTATAAAAGATGGCTATGAGGATTGGCGAAGACATAGATCAGACAACTATGAGAACAACCGGGAGTCTTTGGTCCTTCAGTCTGGCCAATTTCAAGTAAAGAAATGGAAAAATATTCAAGTGGGTGAGGTTCTGAAGATCTGTGCTGATGATACAATTCCTTGTGATATGGTTATGTTAGGAACAAGTGACCCTAGTGGAATTGCCTACATTCAGACAATGAATCTGGATGGCGAGTCAAACCTCAAAACAAGGTTTGCACGCCAGGAAACATCTTCAGCTGTAGCTGAAGGGTGTACAATTATGGGGATCATCAGGTGCGAACAACCTAATCGGAATATCTATGAGTTTACAGCCAACATGGAGTTTAATGGACATACATTTCCCCTGACACAGTCAAATATAGTTTTGCGTGGTTGCCAGCTGAAGAACACGGAGTGGATAATTGGTGTTGTGGTATATGCTGGACAGGAAACAAAGGCAATGTTGAATAGTGCAGCTTCTCCTCCCAAGAGAAGCAAAGTTGAACGCTACATGAACAGAGAAACCCTGTCGTTGTCGATTTTCCTTTTTGTCATGTGTTCAGTTGTGGCTGCTGGCATGGGCACTTGGTTAATACGCCATAAACATCAGCTTGACACATTGCCTTATTACCGGAAACGATTCTTCACTAATTGGGGGAAGCTCAATGGGAAAACCTATAGGTATTATGGGATACCTATGGAGATTCTTTTCTCCTTTTTGAGTTCTGTCATAGTGTTTCAGATAATGATACCGATCTCTCTGTATATTACTGTGGAGTTGGTTCGTTTGGGCCAGTCATATTTCATGATTGAAGACAGGCATATGTTCGACTGTAACTCTGGAGCAAGGTTCCAGTGCAGATCCTTCAATATCAATGAGGATCTGGGTCAAATACGATATATATTTTCAGACAAAACAGGGACACTTACTGAAAATAAGATGGAATTCCGCAGAGCAAGCATATATGGGAGGGATTACGGGAGCCGTGTGCTTGTGGCTGATCAGTTGCAGGAAGAGAATGATACAGGAGGAGGTGTAGCTAGAAAGAGATGGAAGCTAAAATCTGAAGTTGCTGTCGATTCTGAGCTAATGGAGTTGTTGCACAAAGACTTGAGTGAAGATGAGAGGATTGCTGCACATGAGTTTTTCCTTACATTGGCAGCTTGTAATACTGTGGTTCCTATTGTCAGTACTGGTACATCTTCCAGTTGTGCTAAAGGTGATTTGGATGTTGATTCTATTGATTATCAGGGGGAATCCCCAGATGAGCAAGCACTAGTTGCTGCAGCATCTGGTTATAGATATACTCTCTTTGAGCGCACATCAGGGCATATTGCAATTGATGTCAATGGTGAGAAACTAAGATTTCCAAACAATACTATAAAGGTGTTGGTGAAAGGTGCTGATACCTCGATGCTCAGTATACTAGCTAATGATTCTCAAAGAGATGATGAATTGAGACATTCAACTCAGAGGCATCTGAATGAATACTCCTCACAAGGTCTACGTACTCTTGTGGTTGCTGCAAGAGATCTTACAAATGAAGAACTTGAGCAGTGGCAAGGCATGTATGAAGATGCAAGTACCTCATTAAGTGATCGATCCTTAAAACTACGTCAAACAGCAGCTCTTATTGAATCCAACTTAAAGCTACTTGGGGCAACTGCAATTGAGGATAAGCTACAAGATGGTGTGCCAGAAGCTATTGAGTCCCTCAGGCAAGCAGGAATTAAGGTCTGGGTTCTGACTGGAGATAAGCAAGAGACAGCTATTTCAATTGGTGTATCATGCAAGCTCTTGACAGCAGATATGCAACAAATTATCATAAATGGAACTTCTGAGGCCGAATGCAGAAATCTTTTGGTCGATGCTATGGAAAAATACGGTGTGCAGTCATCAAATGAAATAAATCAGAGTTTGAGATGCAAAAGCAATGCTGCAAGTGATTATGTGCTACCTGATGAAGTGAAGACGTCCAATGTGCCAAAATGCCATGCAGGGAAGGAAGAAGGAAAAATCAGTGCCCCATTAGCACTCATAATTGATGGGAACAGTTTGGTATACATTTTGGAGAAAGATCTGCAGTCGGAGCTTTTCGACCTTGCCACTTCCTGTAGTGTTGTTGTATGCTGTCGTGTTGCACCATTGCAGAAAGCTGGAATTGTTGATCTTGTCAAGACCCGTACTGATGATATGACACTGGCTATAGGTGATGGCGCAAATGATGTTTCAATGATCCAAATGGCAGATGTTGGTGTGGGAATTTGCGGTCAGGAGGGGCGTCAAGCTGTGATGGCATCAGATTTTGCTATGGGGCAGTTTCGGTTTTTAAAAAGATTGCTTCTGGTGCATGGACACTGGAATTATCAGCGTATTGGTTATTTGGTTATTTACAACTTCTACCGCAATGCAGTGTTTGTGCTGATGCTATTTTGGTATATATTGTCCACATCATTTTCAACAACTTCTGCTTTGACAGATTATAGTAGTGTTTTCTATTCTCTTATATATACTTCTGTCCCTACAATTGTTGTTGGGGTACTGGACAAAGATCTGAGCCACAGGACATTATTACAATACCCAAAATTGTATGGTTCTGGCCATAGACAGGAAGCATATAATGTACCTCTTTTCTGGATAACAATGCTTGACACCCTATGGCAGAGTCTTGTTCTCTTTTATGTACCCCTGTTCACCTATAAGGAGAGCACCATTGATATATGGAGCATGGGCAGTCTGTGGACCATATCAGTAGTTATCCTTGTTAATGTACATTTGGCAATGGACATTCATCGGTGGGTATTTATCACTCATCTTGCAGTATGGGGATCAATAATCATCACATATGCCTGTGTGGTGATATTGGATTCGATACCTGTTTTTCCCAATTACTGGACTATATACCATTTGGCATGTTCTCCTACCTACTGGATCACCATTTTGCTTATAATAGTTGTAGCCTTGCTCCCTCGTTTTGTGTACAAAGTCGTGCATCATATTTTTTGGGCCTCAGATATCCAGATAGCTAGAGAAGCTGAGATGTTACGTAGGAAACGTAAGCATTTGGGCTCAGAGAAAGATGAAGATTCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1259

Amino Acids

141.7

Weight (kDa)

5.79

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhoLip_ATPase_N PF16209 142 - 207 2e-21 Phospholipid-translocating ATPase N-terminal
Cation_ATPase PF13246 647 - 810 5.3e-14 P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C PF16212 1012 - 1251 1.9e-76 Phospholipid-translocating P-type ATPase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3629
AatII GACGTC 1 cut(s) 2657
AccB7I CCANNNNNTGG 1 cut(s) 3573
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 3 cut(s) 2104, 2185, 2742
AccII CGCG 1 cut(s) 268
AciI CCGC 8 cut(s) 6, 50, 242, 268, 593, 1630, 2949, 3085
AclWI GGATC 8 cut(s) 431, 974, 1536, 1569, 2277, 2911, 3517, 3620
AcoI YGGCCR 3 cut(s) 239, 742, 3280
AcsI RAATTY 2 cut(s) 1625, 2943
AcuI CTGAAG 6 cut(s) 719, 812, 918, 966, 1103, 1767
AcyI GRCGYC 3 cut(s) 287, 2654, 2962
AdeI CACNNNGTG 1 cut(s) 1095
AfiI CCNNNNNNNGG 3 cut(s) 1837, 3289, 3573
AhlI ACTAGT 1 cut(s) 1957
AjnI CCWGG 3 cut(s) 282, 348, 921
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
Alw21I GWGCWC 1 cut(s) 3392
Alw26I GTCTC 5 cut(s) 73, 193, 240, 2134, 2438
AlwI GGATC 8 cut(s) 431, 974, 1536, 1569, 2277, 2911, 3517, 3620
AlwNI CAGNNNCTG 1 cut(s) 1973
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 7 cut(s) 227, 239, 742, 1471, 2523, 3280, 3687
ApoI RAATTY 2 cut(s) 1625, 2943
Asp700I GAANNNNTTC 3 cut(s) 464, 527, 1319
AspLEI GCGC 3 cut(s) 49, 2001, 2900
AspS9I GGNCC 5 cut(s) 227, 730, 1471, 3423, 3687
AsuC2I CCSGG 4 cut(s) 204, 392, 545, 719
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 2 cut(s) 730, 3423
AxyI CCTNAGG 1 cut(s) 2396
BaeGI GKGCMC 2 cut(s) 1265, 2707
BalI TGGCCA 2 cut(s) 744, 3282
BanII GRGCYC 1 cut(s) 3752
BbsI GAAGAC 4 cut(s) 417, 694, 1500, 2657
Bbv12I GWGCWC 1 cut(s) 3392
BceAI ACGGC 3 cut(s) 633, 672, 1650
BciT130I CCWGG 3 cut(s) 284, 350, 923
BciVI GTATCC 1 cut(s) 1365
BclI TGATCA 2 cut(s) 10, 1681
BcnI CCSGG 4 cut(s) 204, 392, 545, 719
BcoDI GTCTC 5 cut(s) 73, 193, 240, 2134, 2438
BcuI ACTAGT 1 cut(s) 1957
BfaI CTAG 8 cut(s) 71, 489, 566, 852, 1722, 1958, 2108, 3708
BfmI CTRYAG 6 cut(s) 939, 1357, 1965, 2762, 2794, 2886
BfoI RGCGCY 1 cut(s) 50
BfuI GTATCC 1 cut(s) 1365
BglII AGATCT 4 cut(s) 795, 2212, 2758, 3232
BmcAI AGTACT 1 cut(s) 1873
Bme1390I CCNGG 7 cut(s) 204, 284, 350, 392, 545, 719, 923
Bme18I GGWCC 2 cut(s) 730, 3423
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 5 cut(s) 227, 730, 1471, 3423, 3687
BmiI GGNNCC 5 cut(s) 106, 354, 1532, 1664, 1860
BmrFI CCNGG 7 cut(s) 204, 284, 350, 392, 545, 719, 923
BpiI GAAGAC 4 cut(s) 417, 694, 1500, 2657
BpmI CTGGAG 3 cut(s) 332, 1542, 2451
BpuEI CTTGAG 2 cut(s) 1813, 2252
BpuMI CCSGG 4 cut(s) 204, 392, 545, 719
Bsa29I ATCGAT 1 cut(s) 2281
BsaAI YACGTR 2 cut(s) 2189, 3728
BsaBI GATNNNNATC 1 cut(s) 1433
BsaHI GRCGYC 3 cut(s) 287, 2654, 2962
BsaI GGTCTC 1 cut(s) 193
BsaJI CCNNGG 2 cut(s) 203, 416
BsaWI WCCGGW 3 cut(s) 31, 220, 1311
BsaXI ACNNNNNCTCC 2 cut(s) 1701, 1731
Bsc4I CCNNNNNNNGG 3 cut(s) 1837, 3289, 3573
Bse21I CCTNAGG 1 cut(s) 2396
Bse3DI GCAATG 8 cut(s) 174, 481, 1146, 1298, 2620, 2825, 3094, 3468
Bse8I GATNNNNATC 1 cut(s) 1433
BseBI CCWGG 3 cut(s) 284, 350, 923
BseCI ATCGAT 1 cut(s) 2281
BseDI CCNNGG 2 cut(s) 203, 416
BseGI GGATG 2 cut(s) 892, 1915
BseJI GATNNNNATC 1 cut(s) 1433
BseLI CCNNNNNNNGG 3 cut(s) 1837, 3289, 3573
BseMI GCAATG 8 cut(s) 174, 481, 1146, 1298, 2620, 2825, 3094, 3468
BseMII CTCAG 9 cut(s) 1755, 2113, 2165, 2410, 2511, 3227, 3705, 3708, 3765
BseRI GAGGAG 3 cut(s) 1152, 1725, 2164
BseSI GKGCMC 2 cut(s) 1265, 2707
BseX3I CGGCCG 1 cut(s) 239
BsgI GTGCAG 6 cut(s) 77, 293, 1173, 1558, 1800, 2591
Bsh1236I CGCG 1 cut(s) 268
Bsh1285I CGRYCG 1 cut(s) 242
BshFI GGCC 7 cut(s) 229, 241, 744, 1473, 2525, 3282, 3689
BshVI ATCGAT 1 cut(s) 2281
BsiEI CGRYCG 1 cut(s) 242
BsiHKAI GWGCWC 1 cut(s) 3392
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 8 cut(s) 32, 203, 221, 230, 392, 544, 718, 1312
BslFI GGGAC 4 cut(s) 319, 1615, 2377, 3185
BslI CCNNNNNNNGG 3 cut(s) 1837, 3289, 3573
BsmAI GTCTC 5 cut(s) 73, 193, 240, 2134, 2438
BsmFI GGGAC 4 cut(s) 319, 1615, 2377, 3185
BsmI GAATGC 1 cut(s) 2534
BsnI GGCC 7 cut(s) 229, 241, 744, 1473, 2525, 3282, 3689
Bso31I GGTCTC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 4 cut(s) 1265, 2707, 3392, 3752
Bsp1407I TGTACA 3 cut(s) 953, 3451, 3660
BspACI CCGC 8 cut(s) 6, 50, 242, 268, 593, 1630, 2949, 3085
BspANI GGCC 7 cut(s) 229, 241, 744, 1473, 2525, 3282, 3689
BspCNI CTCAG 9 cut(s) 1756, 2112, 2164, 2409, 2512, 3228, 3704, 3709, 3764
BspDI ATCGAT 1 cut(s) 2281
BspFNI CGCG 1 cut(s) 268
BspHI TCATGA 1 cut(s) 1485
BspLI GGNNCC 5 cut(s) 106, 354, 1532, 1664, 1860
BspMAI CTGCAG 2 cut(s) 1969, 2766
BspPI GGATC 8 cut(s) 431, 974, 1536, 1569, 2277, 2911, 3517, 3620
BspTNI GGTCTC 1 cut(s) 193
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 8 cut(s) 174, 481, 1146, 1298, 2620, 2825, 3094, 3468
BsrGI TGTACA 3 cut(s) 953, 3451, 3660
BssECI CCNNGG 2 cut(s) 203, 416
BssNAI GTATAC 2 cut(s) 2105, 2743
BssNI GRCGYC 3 cut(s) 287, 2654, 2962
BssT1I CCWWGG 1 cut(s) 416
Bst1107I GTATAC 2 cut(s) 2105, 2743
Bst2UI CCWGG 3 cut(s) 284, 350, 923
Bst4CI ACNGT 8 cut(s) 191, 314, 1053, 1453, 1514, 1855, 2567, 2735
Bst6I CTCTTC 2 cut(s) 437, 1689
BstACI GRCGYC 3 cut(s) 287, 2654, 2962
BstAPI GCANNNNNTGC 2 cut(s) 94, 1961
BstAUI TGTACA 3 cut(s) 953, 3451, 3660
BstBAI YACGTR 2 cut(s) 2189, 3728
BstC8I GCNNGC 7 cut(s) 919, 1079, 1255, 1639, 1953, 2403, 2474
BstF5I GGATG 2 cut(s) 892, 1915
BstFNI CGCG 1 cut(s) 268
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 3 cut(s) 49, 2001, 2900
BstMAI GTCTC 5 cut(s) 73, 193, 240, 2134, 2438
BstMCI CGRYCG 1 cut(s) 242
BstNI CCWGG 3 cut(s) 284, 350, 923
BstNSI RCATGY 2 cut(s) 2251, 3597
BstSCI CCNGG 7 cut(s) 202, 282, 348, 390, 543, 717, 921
BstSFI CTRYAG 6 cut(s) 939, 1357, 1965, 2762, 2794, 2886
BstSLI GKGCMC 2 cut(s) 1265, 2707
BstSNI TACGTA 2 cut(s) 2189, 3728
BstUI CGCG 1 cut(s) 268
BstV2I GAAGAC 4 cut(s) 417, 694, 1500, 2657
BstX2I RGATCY 6 cut(s) 795, 1541, 1561, 2212, 2758, 3232
BstYI RGATCY 6 cut(s) 795, 1541, 1561, 2212, 2758, 3232
BstZ17I GTATAC 2 cut(s) 2105, 2743
BstZI CGGCCG 1 cut(s) 239
Bsu15I ATCGAT 1 cut(s) 2281
Bsu36I CCTNAGG 1 cut(s) 2396
BsuI GTATCC 1 cut(s) 1365
BsuRI GGCC 7 cut(s) 229, 241, 744, 1473, 2525, 3282, 3689
BsuTUI ATCGAT 1 cut(s) 2281
BtsCI GGATG 2 cut(s) 892, 1915
BtsI GCAGTG 2 cut(s) 2243, 3099
BtsIMutI CAGTG 7 cut(s) 468, 1541, 2243, 2707, 2879, 3037, 3099
Cac8I GCNNGC 7 cut(s) 919, 1079, 1255, 1639, 1953, 2403, 2474
CaiI CAGNNNCTG 1 cut(s) 1973
CciI TCATGA 1 cut(s) 1485
CfoI GCGC 3 cut(s) 49, 2001, 2900
Cfr13I GGNCC 5 cut(s) 227, 730, 1471, 3423, 3687
ClaI ATCGAT 1 cut(s) 2281
CseI GACGC 3 cut(s) 276, 321, 2951
DraI TTTAAA 1 cut(s) 3012
DraIII CACNNNGTG 1 cut(s) 1095
EaeI YGGCCR 3 cut(s) 239, 742, 3280
EagI CGGCCG 1 cut(s) 239
Eam1104I CTCTTC 2 cut(s) 437, 1689
EarI CTCTTC 2 cut(s) 437, 1689
EciI GGCGGA 1 cut(s) 582
EclXI CGGCCG 1 cut(s) 239
Eco105I TACGTA 2 cut(s) 2189, 3728
Eco130I CCWWGG 1 cut(s) 416
Eco24I GRGCYC 1 cut(s) 3752
Eco31I GGTCTC 1 cut(s) 193
Eco32I GATATC 2 cut(s) 397, 3697
Eco47I GGWCC 2 cut(s) 730, 3423
Eco52I CGGCCG 1 cut(s) 239
Eco57I CTGAAG 6 cut(s) 719, 812, 918, 966, 1103, 1767
Eco81I CCTNAGG 1 cut(s) 2396
Eco88I CYCGRG 1 cut(s) 83
EcoRI GAATTC 1 cut(s) 1625
EcoRII CCWGG 3 cut(s) 282, 348, 921
EcoRV GATATC 2 cut(s) 397, 3697
EcoT14I CCWWGG 1 cut(s) 416
EcoT38I GRGCYC 1 cut(s) 3752
ErhI CCWWGG 1 cut(s) 416
FaqI GGGAC 4 cut(s) 319, 1615, 2377, 3185
FauNDI CATATG 2 cut(s) 1503, 3526
FbaI TGATCA 2 cut(s) 10, 1681
FblI GTMKAC 3 cut(s) 2104, 2185, 2742
FokI GGATG 2 cut(s) 899, 1922
FriOI GRGCYC 1 cut(s) 3752
FspBI CTAG 8 cut(s) 71, 489, 566, 852, 1722, 1958, 2108, 3708
GlaI GCGC 3 cut(s) 48, 2000, 2899
GsuI CTGGAG 3 cut(s) 332, 1542, 2451
HaeII RGCGCY 1 cut(s) 50
HaeIII GGCC 7 cut(s) 229, 241, 744, 1473, 2525, 3282, 3689
HapII CCGG 8 cut(s) 32, 203, 221, 230, 392, 544, 718, 1312
HgaI GACGC 3 cut(s) 276, 321, 2951
HhaI GCGC 3 cut(s) 49, 2001, 2900
Hin1I GRCGYC 3 cut(s) 287, 2654, 2962
Hin6I GCGC 3 cut(s) 47, 1999, 2898
HinP1I GCGC 3 cut(s) 47, 1999, 2898
HpaII CCGG 8 cut(s) 32, 203, 221, 230, 392, 544, 718, 1312
Hpy99I CGWCG 1 cut(s) 292
HpyAV CCTTC 6 cut(s) 126, 743, 941, 1555, 2677, 2684
HpyCH4III ACNGT 8 cut(s) 191, 314, 1053, 1453, 1514, 1855, 2567, 2735
HpyCH4IV ACGT 5 cut(s) 2188, 2296, 2654, 3727, 3736
HpySE526I ACGT 5 cut(s) 2188, 2296, 2654, 3727, 3736
Hsp92I GRCGYC 3 cut(s) 287, 2654, 2962
HspAI GCGC 3 cut(s) 47, 1999, 2898
Ksp22I TGATCA 2 cut(s) 10, 1681
LmnI GCTCC 6 cut(s) 358, 1523, 1662, 2770, 3405, 3651
MaeI CTAG 8 cut(s) 71, 489, 566, 852, 1722, 1958, 2108, 3708
MaeII ACGT 5 cut(s) 2188, 2296, 2654, 3727, 3736
MaeIII GTNAC 5 cut(s) 386, 652, 845, 1514, 3723
MbiI CCGCTC 1 cut(s) 6
MfeI CAATTG 4 cut(s) 2019, 2349, 2457, 3207
MflI RGATCY 6 cut(s) 795, 1541, 1561, 2212, 2758, 3232
MhlI GDGCHC 4 cut(s) 1265, 2707, 3392, 3752
MlsI TGGCCA 2 cut(s) 744, 3282
MluNI TGGCCA 2 cut(s) 744, 3282
MlyI GAGTC 6 cut(s) 75, 242, 731, 902, 2399, 3355
MmeI TCCRAC 2 cut(s) 2346, 2748
Mox20I TGGCCA 2 cut(s) 744, 3282
MroXI GAANNNNTTC 3 cut(s) 464, 527, 1319
MscI TGGCCA 2 cut(s) 744, 3282
MslI CAYNNNNRTG 1 cut(s) 2163
Msp20I TGGCCA 2 cut(s) 744, 3282
MspA1I CMGCKG 2 cut(s) 938, 1081
MspI CCGG 8 cut(s) 32, 203, 221, 230, 392, 544, 718, 1312
MspR9I CCNGG 7 cut(s) 204, 284, 350, 392, 545, 719, 923
MunI CAATTG 4 cut(s) 2019, 2349, 2457, 3207
Mva1269I GAATGC 1 cut(s) 2534
MvaI CCWGG 3 cut(s) 284, 350, 923
MvnI CGCG 1 cut(s) 268
NciI CCSGG 4 cut(s) 204, 392, 545, 719
NdeI CATATG 2 cut(s) 1503, 3526
NlaIV GGNNCC 5 cut(s) 106, 354, 1532, 1664, 1860
NmeAIII GCCGAG 2 cut(s) 100, 217
NmuCI GTSAC 3 cut(s) 386, 652, 845
NspI RCATGY 2 cut(s) 2251, 3597
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 1 cut(s) 1485
PcsI WCGNNNNNNNCGW 1 cut(s) 3733
PctI GAATGC 1 cut(s) 2534
PdmI GAANNNNTTC 3 cut(s) 464, 527, 1319
PflFI GACNNNGTC 1 cut(s) 1051
PflMI CCANNNNNTGG 1 cut(s) 3573
PleI GAGTC 6 cut(s) 75, 241, 730, 901, 2398, 3354
PpsI GAGTC 6 cut(s) 75, 241, 730, 901, 2398, 3354
Ppu21I YACGTR 2 cut(s) 2189, 3728
PsiI TTATAA 1 cut(s) 3629
Psp6I CCWGG 3 cut(s) 282, 348, 921
PspGI CCWGG 3 cut(s) 282, 348, 921
PspN4I GGNNCC 5 cut(s) 106, 354, 1532, 1664, 1860
PspPI GGNCC 5 cut(s) 227, 730, 1471, 3423, 3687
PspXI VCTCGAGB 1 cut(s) 83
PstI CTGCAG 2 cut(s) 1969, 2766
PstNI CAGNNNCTG 1 cut(s) 1973
PsuI RGATCY 6 cut(s) 795, 1541, 1561, 2212, 2758, 3232
PsyI GACNNNGTC 1 cut(s) 1051
PvuII CAGCTG 2 cut(s) 938, 1081
RseI CAYNNNNRTG 1 cut(s) 2163
Sau96I GGNCC 5 cut(s) 227, 730, 1471, 3423, 3687
ScaI AGTACT 1 cut(s) 1873
SchI GAGTC 6 cut(s) 75, 242, 731, 902, 2399, 3355
ScrFI CCNGG 7 cut(s) 204, 284, 350, 392, 545, 719, 923
SduI GDGCHC 4 cut(s) 1265, 2707, 3392, 3752
SfcI CTRYAG 6 cut(s) 939, 1357, 1965, 2762, 2794, 2886
Sfr274I CTCGAG 1 cut(s) 83
SinI GGWCC 2 cut(s) 730, 3423
SlaI CTCGAG 1 cut(s) 83
SmiMI CAYNNNNRTG 1 cut(s) 2163
SmlI CTYRAG 3 cut(s) 83, 1792, 2231
SmoI CTYRAG 3 cut(s) 83, 1792, 2231
SnaBI TACGTA 2 cut(s) 2189, 3728
SpeI ACTAGT 1 cut(s) 1957
SsiI CCGC 8 cut(s) 6, 50, 242, 268, 593, 1630, 2949, 3085
SspI AATATT 1 cut(s) 772
SspMI CTAG 8 cut(s) 71, 489, 566, 852, 1722, 1958, 2108, 3708
StyD4I CCNGG 7 cut(s) 202, 282, 348, 390, 543, 717, 921
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 8 cut(s) 191, 314, 1053, 1453, 1514, 1855, 2567, 2735
TaiI ACGT 5 cut(s) 2191, 2299, 2657, 3730, 3739
TaqI TCGA 9 cut(s) 84, 1217, 1509, 1758, 2093, 2281, 2547, 2778, 3551
TatI WGTACW 5 cut(s) 495, 953, 1871, 3451, 3660
TauI GCSGC 3 cut(s) 52, 244, 271
TscAI CASTG 7 cut(s) 475, 1541, 2243, 2707, 2886, 3044, 3099
TseFI GTSAC 3 cut(s) 386, 652, 845
Tsp45I GTSAC 3 cut(s) 386, 652, 845
TspGWI ACGGA 1 cut(s) 1106
TspRI CASTG 7 cut(s) 475, 1541, 2243, 2707, 2886, 3044, 3099
Tth111I GACNNNGTC 1 cut(s) 1051
Van91I CCANNNNNTGG 1 cut(s) 3573
VpaK11BI GGWCC 2 cut(s) 730, 3423
XapI RAATTY 2 cut(s) 1625, 2943
XceI RCATGY 2 cut(s) 2251, 3597
XcmI CCANNNNNNNNNTGG 1 cut(s) 356
XhoI CTCGAG 1 cut(s) 83
XmiI GTMKAC 3 cut(s) 2104, 2185, 2742
XmnI GAANNNNTTC 3 cut(s) 464, 527, 1319
XspI CTAG 8 cut(s) 71, 489, 566, 852, 1722, 1958, 2108, 3708
ZraI GACGTC 1 cut(s) 2655
ZrmI AGTACT 1 cut(s) 1873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.