Rroxscaffold_6G00419600

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0009696
Physical Location & Seq
Reverse (-)
41007749 .. 41015502
7754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00419600.1

Sequence Viewer

Length: 3873 bp
ATGAGCGGTGAGCATCCGTTGCTGTCATCACCGGATTCTCCATCAAAGCCACTTGTGCCGCCCTCTTCTCTAGCTGAGAGACTCGAGCAGCTCTGCCTTGATGAATCCAGTTCTTCTTCCATTCTTGACGGCATTGATGATGGCCAAGGTGAATCCTTGTTAGCAAGGGAATACACTGCTGTTCCTGACTGCGGTGTGAAACCAGGAAAGCAGTTTTTTCCTGTAGAGCGTGAGTGCCGTCCGCAGTTTCCGTTGGAATACCCCACGCGGCATAAAAAACGCCCGGTATCGTGGGGTACAATGGAACTGCATAGTATCAATAGTAATCCAGCGTCCCATGATGTCCCCCAGGCTCCATCTAGGGTTCTGGAAAAGGCGAACAAGTCTCAGGGGAGTCACCACAAAGGCATTCAATTTGAAGATAACCTCCTACATGAAGACAATCCAAGGTTGATCTATATTGATGATCCAAAGAAAACAAATGACAAGTATGAGTTCACTGGCAATGAGATTCGAACCAGCAAGTACACCTTAATCACCTTCTTGCCCAAGAATGTTTTCATTCAGTTTCACCGGGTTGCTTATTTGTATTTTCTAGCTATTGCCGTTCTGAATCAGCTTCCGCCTCTTGCTGTGTTTGGAAGAACGGTGTCTCTTTTTCCCCTCCTATTTGTGCTGTGTGTCACTGCTATAAAAGATGGCTATGAGGATTGGCGAAGACATAGATCAGACAGGAATGAAAACAACCGGGAGTCTCTGATCCTTCAATCTGGGCAATTTCAACCAAAGAAGTGGAAAAATATTCAAGTGGGCGAGGTTCTGAAGATTCGTGCTGATGATACAATTCCTTGTGATATAGTTTTGTTAGGAACAAGTGACCCTAGTGGAATTGCCTACATTCAGACAATGAATCTGGATGGTGAGTCAAACCTCAAAACAAGGTATGCACGCCAGGAAACATCTTCAGCTGTAGGTTCAGCAGTAGCCGATGGGAGTGCAATTATGGGGATCATCAGATGCGAACAACCAAATCGGAATATCTATGAGTTTACAGCCAACATGGAGTTTAATGGACATAGATTTCCCCTAACACAGTCAAACATAGTTTTGCGTGGTTGCCAGCTGAAGAACACGGAGTGGATAATTGGTGTTGTGGTATATGCTGGACAGGAAACAAAGGCAATGTTGAATAGTGCAGCTTCTCCTTCCAAGAGAAGCAAACTTGAAGGCTACATGAACAGAGAAACCTTGTGGTTGTCAATTTTCCTTTTTGTCATGTGTTCAGTTGTGGCTGCTGGCATGGGCACGTGGTTAATACGCCATAAATATCAGGTTGATACGTTGCCTTATTACCGGAAAAGATTCTTCACTAATTGGGGGAAGCTTAATGGGAAAACCTATAAATATTATGGGATACCTATGGAGATTTTTTTCTCCTTTTTGAGTTCTGTCATAGTGTTTCAGATAATGATACCAATCGCTCTGTATATTAGTATTGAGTTGGTTCGTTTGGGCCAGTCATATTTCATGATTGAAGACAGGCATATGTTCGACTGTAGCTCTGGCTCAAGGTTCCAGTGCAGATCCTTGAATATCAATGAGGATCTGGGTCAAATACGATATATATTTTCAGACAAAACAGGGACACTGACTGAAAATAAGATGGCATTCCGCAGAGCAAGCATATATGGGAGGGATTATGGGAGCCGTGTGCTTGTGGCTGATCAATTGCAGGAAAAAAATAATACAGGAGAAGGTTTAGCTAGAAAGAGATGGAAGCTTAAATCTGAAGTTGCTGTCGATTATGAGCTCATGGAGTTGTTGCACAAAGACTTGAGTGAAGATGAGAGGATTGCTGCACATGAGTTTTTCCTTACGTTGGCAGCTTGCAATACTGTGGTTCCTATAGTCAGTACTAGTACATCTTCCATTTGTGCTAAGGGTGATGTTGATTCTATTGATTATCAGGGGGAATCCCCGGACGAGCAAGCACTAGTTACTGCAGCCTCTGGTTATAGATATACTCTCTTTGAGCGCACATCGGGACATATTGCAATTGATGTCAATGGTGAGAAACTAAGGTTGGATGTATTGGGTCTGCATGAATTTGACAGTGTGCGAAAACGAATGTCAGTTGTTATCAGATTTCCAAACAATACTATAAAGGTATTGGTGAAAGGTGCTGATACCCCTATGCTCAGCATATTAGCGAATGACTCTGAAAGAGATGATGAACTGAGACATTCAACTCAGAACCATTTGAGTGAATATTCCTCACATGGTTTACGTACTCTTGTGGTTGCTGCAAGAGATCTTACAAATGAAGAACTTGAACAGTGGCAAAGCATGTATGAAGATGCAAGTACCTCATTGACTGATCGATCCTTAAAACTACGTCAAACAGCAGCTCTTATTGAATCCAACTTAAAGCTACTTGGGGCAACTGCAATTGAGGATAGGCTACAAGATGGTGTGCCAGAAGCTATTGAGTCCCTCAGGCAAGCAGGAATTAAGGTCTGGGTTTTGACTGGAGATAAGCAAGAGACAGCTATTTCAATTGGTGTATCATGCAAACTGTTGACAGCAGATATGCAACAAATTATCATAAATGGAACTTCTGAGGCTGAATGCAGAAATCTTTTGGTTGATGCTATGGAAAAATATGGTGTGCAGTCATCAAATAAAAGAAATCAGAGTTCAAAATGCAAAAGCAATGCTGCAAGTGATTATCTTGAGTTACCTGATGATGCAAAGACATCTAATGTGCCAAAATGGCAAGAAGCAAAGGAAGAAGGAAAGATAAGTGTGCCATTAGCACTCATAATTGATGGGAACAGTTTGCTTTTCGACCTTGCCACTTCCTGTAGTGTGGTTGTATGCTGTCGTGTTGCACCATTGCAGAAAGCTGGAATTGTTGATCTAATTAAGACCCGCACTGATGATATGACACTGGCTATAGGTGATGGGGCAAATGATGTTTCTATGATCCAAATGGCGGATGTTGGTGTTGGAATTTGTGGTCAGGAGGGTCGTCAAGCTGTGATGGCATCAGACTTTGCCATGGGACAGTTTCGGTTTTTGAAAAGATTGCTTCTGGTGCATGGACACTGGAATTATCAGCGTATTGGTTATTTGATTCTTTACAACTTCTACCGCAACGCAGTTTTTGTGCTGATGCTATTTTGGTATATATTGTCCACAGCATTTTCAACAACTTCGGCGTTGACAGATTGGAGTAGTGTTTTCTATTCTCTTATATATACGTCTATCCCTACAATTATTGTTGGTGTACTGGACAAAGATCTGAGCCACAAGACCCTGTTACAATACCCAAAATTGTATGGTGCTGGCCATAGACAGGAAGCATATAATCCAAATCTTTTCTGGATAACGATGCTTGACACCCTATGGCAGAGTCTTGTTCTCTTCTATGTACCCCAGTTCACCTATAAGGAGAGCACAATAGACATATGGAGCATGGGCAGTTTGTGGACCATAGCAGTAGTTATCCTTGTTAATGTACATTTGGCAATGGACATTCATCGGTGGGTATTTATCACTCATATTGCAGTATGGGGATCAATAATCATCACATATGCCTGTGTGGTGATATTGGATTCCATACCTGTTTATCCCAATTACTGGTTAGCTTCCTTGACTTCTGCTGTTCCTTATATCCAGATAGCTAGAGAAGCTGAGATATTAAGTAGGAAACATAAGCATTTGGGCTCAGAAAAAGATGAAGATTCAGTGGGGTGCGGTTGTGAAATGTTAAAGATAATTGCCTTAGCCCCGTCTGCAGATCATCAGCATTTGACAGCAAAAGGGAGTGCAGTTGCAGAAGAAGAAGAGGGGATATGGTGGATCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1290

Amino Acids

145.17

Weight (kDa)

6.02

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhoLip_ATPase_N PF16209 152 - 217 4.1e-22 Phospholipid-translocating ATPase N-terminal
Cation_ATPase PF13246 658 - 822 6.4e-14 P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C PF16212 1014 - 1214 9.8e-69 Phospholipid-translocating P-type ATPase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 3642
AccBSI CCGCTC 1 cut(s) 6
AccII CGCG 1 cut(s) 268
AclWI GGATC 9 cut(s) 461, 754, 1016, 1578, 1611, 2376, 2980, 3586, 3872
AcoI YGGCCR 2 cut(s) 142, 3349
AcsI RAATTY 2 cut(s) 2105, 3012
AcuI CTGAAG 4 cut(s) 842, 948, 1145, 1809
AcvI CACGTG 1 cut(s) 1308
AdeI CACNNNGTG 1 cut(s) 1137
AfaI GTAC 9 cut(s) 298, 527, 1915, 1921, 2290, 2365, 3291, 3435, 3522
AfiI CCNNNNNNNGG 5 cut(s) 191, 1879, 2995, 3358, 3642
AhlI ACTAGT 2 cut(s) 1916, 1993
AjnI CCWGG 3 cut(s) 202, 348, 951
AjuI GAANNNNNNNTTGG 4 cut(s) 542, 574, 2066, 2098
Alw21I GWGCWC 2 cut(s) 1812, 3461
Alw26I GTCTC 6 cut(s) 73, 390, 657, 759, 2233, 2537
AlwI GGATC 9 cut(s) 461, 754, 1016, 1578, 1611, 2376, 2980, 3586, 3872
AlwNI CAGNNNCTG 1 cut(s) 2009
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 3 cut(s) 142, 1513, 3349
ApeKI GCWGC 9 cut(s) 88, 1196, 1292, 1856, 1883, 2003, 2303, 2405, 2717
ApoI RAATTY 2 cut(s) 2105, 3012
Asp700I GAANNNNTTC 2 cut(s) 557, 1361
AspLEI GCGC 1 cut(s) 2037
AspS9I GGNCC 2 cut(s) 1513, 3492
AsuC2I CCSGG 4 cut(s) 284, 575, 749, 1979
AsuII TTCGAA 1 cut(s) 514
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 1 cut(s) 3492
AxyI CCTNAGG 1 cut(s) 2495
BaeGI GKGCMC 1 cut(s) 1307
BalI TGGCCA 2 cut(s) 144, 3351
BanII GRGCYC 2 cut(s) 1812, 3731
BbrPI CACGTG 1 cut(s) 1308
BbsI GAAGAC 3 cut(s) 444, 724, 1542
Bbv12I GWGCWC 2 cut(s) 1812, 3461
BbvI GCAGC 9 cut(s) 100, 1208, 1279, 1843, 1895, 2015, 2290, 2417, 2704
BceAI ACGGC 4 cut(s) 145, 222, 590, 1692
BcgI CGANNNNNNTGC 2 cut(s) 977, 1011
BciT130I CCWGG 3 cut(s) 204, 350, 953
BciVI GTATCC 1 cut(s) 1407
BclI TGATCA 1 cut(s) 1723
BcnI CCSGG 4 cut(s) 284, 575, 749, 1979
BcoDI GTCTC 6 cut(s) 73, 390, 657, 759, 2233, 2537
BcuI ACTAGT 2 cut(s) 1916, 1993
BfaI CTAG 9 cut(s) 71, 360, 596, 882, 1764, 1917, 1994, 3687, 3871
BfmI CTRYAG 8 cut(s) 222, 969, 1555, 1905, 2001, 2863, 2955, 3798
BfuI GTATCC 1 cut(s) 1407
BglI GCCNNNNNGGC 1 cut(s) 2773
BglII AGATCT 2 cut(s) 2311, 3301
BlpI GCTNAGC 1 cut(s) 2198
BmcAI AGTACT 1 cut(s) 1915
Bme1390I CCNGG 7 cut(s) 204, 284, 350, 575, 749, 953, 1979
Bme18I GGWCC 1 cut(s) 3492
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 2 cut(s) 1513, 3492
BmiI GGNNCC 4 cut(s) 354, 1574, 1706, 1902
BmrFI CCNGG 7 cut(s) 204, 284, 350, 575, 749, 953, 1979
BmrI ACTGGG 1 cut(s) 3433
BmsI GCATC 8 cut(s) 22, 1007, 2347, 2638, 2737, 3056, 3165, 3384
BmuI ACTGGG 1 cut(s) 3433
BpiI GAAGAC 3 cut(s) 444, 724, 1542
BpmI CTGGAG 1 cut(s) 2550
Bpu10I CCTNAGC 2 cut(s) 1938, 3787
Bpu1102I GCTNAGC 1 cut(s) 2198
Bpu14I TTCGAA 1 cut(s) 514
BpuEI CTTGAG 3 cut(s) 1552, 1855, 2753
BpuMI CCSGG 4 cut(s) 284, 575, 749, 1979
Bsa29I ATCGAT 1 cut(s) 2380
BsaAI YACGTR 2 cut(s) 1308, 2288
BsaBI GATNNNNATC 1 cut(s) 1475
BsaJI CCNNGG 5 cut(s) 145, 348, 446, 1977, 3060
BsaWI WCCGGW 2 cut(s) 31, 1353
Bsc4I CCNNNNNNNGG 5 cut(s) 191, 1879, 2995, 3358, 3642
Bse21I CCTNAGG 1 cut(s) 2495
Bse3DI GCAATG 5 cut(s) 511, 1188, 2719, 2894, 3537
Bse8I GATNNNNATC 1 cut(s) 1475
BseBI CCWGG 3 cut(s) 204, 350, 953
BseCI ATCGAT 1 cut(s) 2380
BseDI CCNNGG 5 cut(s) 145, 348, 446, 1977, 3060
BseGI GGATG 4 cut(s) 13, 922, 2092, 3004
BseJI GATNNNNATC 1 cut(s) 1475
BseLI CCNNNNNNNGG 5 cut(s) 191, 1879, 2995, 3358, 3642
BseMI GCAATG 5 cut(s) 511, 1188, 2719, 2894, 3537
BseSI GKGCMC 1 cut(s) 1307
BseXI GCAGC 9 cut(s) 100, 1208, 1279, 1843, 1895, 2015, 2290, 2417, 2704
BsgI GTGCAG 5 cut(s) 1215, 1600, 1842, 2690, 3852
Bsh1236I CGCG 1 cut(s) 268
BshFI GGCC 3 cut(s) 144, 1515, 3351
BshVI ATCGAT 1 cut(s) 2380
BsiHKAI GWGCWC 2 cut(s) 1812, 3461
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 6 cut(s) 32, 284, 574, 748, 1354, 1979
BslFI GGGAC 6 cut(s) 319, 329, 1657, 2058, 2476, 3078
BslI CCNNNNNNNGG 5 cut(s) 191, 1879, 2995, 3358, 3642
BsmAI GTCTC 6 cut(s) 73, 390, 657, 759, 2233, 2537
BsmFI GGGAC 6 cut(s) 319, 329, 1657, 2058, 2476, 3078
BsmI GAATGC 3 cut(s) 408, 1667, 2633
BsnI GGCC 3 cut(s) 144, 1515, 3351
BsoBI CYCGRG 1 cut(s) 83
Bsp119I TTCGAA 1 cut(s) 514
Bsp1286I GDGCHC 4 cut(s) 1307, 1812, 3461, 3731
Bsp1407I TGTACA 1 cut(s) 3520
Bsp1720I GCTNAGC 1 cut(s) 2198
Bsp19I CCATGG 1 cut(s) 3060
BspANI GGCC 3 cut(s) 144, 1515, 3351
BspDI ATCGAT 1 cut(s) 2380
BspFNI CGCG 1 cut(s) 268
BspHI TCATGA 1 cut(s) 1527
BspLI GGNNCC 4 cut(s) 354, 1574, 1706, 1902
BspMAI CTGCAG 2 cut(s) 2005, 3802
BspPI GGATC 9 cut(s) 461, 754, 1016, 1578, 1611, 2376, 2980, 3586, 3872
BspT104I TTCGAA 1 cut(s) 514
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 5 cut(s) 511, 1188, 2719, 2894, 3537
BsrGI TGTACA 1 cut(s) 3520
BssECI CCNNGG 5 cut(s) 145, 348, 446, 1977, 3060
BssT1I CCWWGG 3 cut(s) 145, 446, 3060
Bst2UI CCWGG 3 cut(s) 204, 350, 953
Bst4CI ACNGT 9 cut(s) 649, 1095, 1556, 1897, 2114, 2337, 2577, 2837, 3069
Bst6I CTCTTC 3 cut(s) 70, 3431, 3843
BstAPI GCANNNNNTGC 1 cut(s) 19
BstAUI TGTACA 1 cut(s) 3520
BstBAI YACGTR 2 cut(s) 1308, 2288
BstBI TTCGAA 1 cut(s) 514
BstC8I GCNNGC 8 cut(s) 949, 1121, 1297, 1681, 1888, 1989, 2502, 3349
BstDSI CCRYGG 1 cut(s) 3060
BstF5I GGATG 4 cut(s) 13, 922, 2092, 3004
BstFNI CGCG 1 cut(s) 268
BstHHI GCGC 1 cut(s) 2037
BstMAI GTCTC 6 cut(s) 73, 390, 657, 759, 2233, 2537
BstMWI GCNNNNNNNGC 8 cut(s) 19, 55, 1680, 2773, 3044, 3097, 3692, 3797
BstNI CCWGG 3 cut(s) 204, 350, 953
BstNSI RCATGY 1 cut(s) 2350
BstSCI CCNGG 7 cut(s) 202, 282, 348, 573, 747, 951, 1977
BstSFI CTRYAG 8 cut(s) 222, 969, 1555, 1905, 2001, 2863, 2955, 3798
BstSLI GKGCMC 1 cut(s) 1307
BstSNI TACGTA 1 cut(s) 2288
BstUI CGCG 1 cut(s) 268
BstV1I GCAGC 9 cut(s) 100, 1208, 1279, 1843, 1895, 2015, 2290, 2417, 2704
BstV2I GAAGAC 3 cut(s) 444, 724, 1542
BstX2I RGATCY 5 cut(s) 1583, 1603, 2311, 3301, 3864
BstXI CCANNNNNNTGG 1 cut(s) 792
BstYI RGATCY 5 cut(s) 1583, 1603, 2311, 3301, 3864
Bsu15I ATCGAT 1 cut(s) 2380
Bsu36I CCTNAGG 1 cut(s) 2495
BsuI GTATCC 1 cut(s) 1407
BsuRI GGCC 3 cut(s) 144, 1515, 3351
BsuTUI ATCGAT 1 cut(s) 2380
BtgI CCRYGG 1 cut(s) 3060
BtsCI GGATG 4 cut(s) 13, 922, 2092, 3004
BtsI GCAGTG 2 cut(s) 174, 684
Cac8I GCNNGC 8 cut(s) 949, 1121, 1297, 1681, 1888, 1989, 2502, 3349
CaiI CAGNNNCTG 1 cut(s) 2009
CciI TCATGA 1 cut(s) 1527
CfoI GCGC 1 cut(s) 2037
Cfr13I GGNCC 2 cut(s) 1513, 3492
ClaI ATCGAT 1 cut(s) 2380
CseI GACGC 1 cut(s) 321
Csp6I GTAC 9 cut(s) 297, 526, 1914, 1920, 2289, 2364, 3290, 3434, 3521
CviQI GTAC 9 cut(s) 297, 526, 1914, 1920, 2289, 2364, 3290, 3434, 3521
DraIII CACNNNGTG 1 cut(s) 1137
EaeI YGGCCR 2 cut(s) 142, 3349
Eam1104I CTCTTC 3 cut(s) 70, 3431, 3843
EarI CTCTTC 3 cut(s) 70, 3431, 3843
EciI GGCGGA 2 cut(s) 612, 3011
Ecl136II GAGCTC 1 cut(s) 1810
Eco105I TACGTA 1 cut(s) 2288
Eco130I CCWWGG 3 cut(s) 145, 446, 3060
Eco24I GRGCYC 2 cut(s) 1812, 3731
Eco47I GGWCC 1 cut(s) 3492
Eco53kI GAGCTC 1 cut(s) 1810
Eco57I CTGAAG 4 cut(s) 842, 948, 1145, 1809
Eco72I CACGTG 1 cut(s) 1308
Eco81I CCTNAGG 1 cut(s) 2495
Eco88I CYCGRG 1 cut(s) 83
EcoICRI GAGCTC 1 cut(s) 1810
EcoRII CCWGG 3 cut(s) 202, 348, 951
EcoT14I CCWWGG 3 cut(s) 145, 446, 3060
EcoT38I GRGCYC 2 cut(s) 1812, 3731
ErhI CCWWGG 3 cut(s) 145, 446, 3060
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FaqI GGGAC 6 cut(s) 319, 329, 1657, 2058, 2476, 3078
FauI CCCGC 1 cut(s) 2939
FauNDI CATATG 3 cut(s) 1545, 3470, 3595
FbaI TGATCA 1 cut(s) 1723
FokI GGATG 3 cut(s) 929, 2099, 3011
FriOI GRGCYC 2 cut(s) 1812, 3731
FspBI CTAG 9 cut(s) 71, 360, 596, 882, 1764, 1917, 1994, 3687, 3871
GlaI GCGC 1 cut(s) 2036
GsuI CTGGAG 1 cut(s) 2550
HaeIII GGCC 3 cut(s) 144, 1515, 3351
HapII CCGG 6 cut(s) 32, 284, 574, 748, 1354, 1979
HgaI GACGC 1 cut(s) 321
HhaI GCGC 1 cut(s) 2037
Hin6I GCGC 1 cut(s) 2035
HinP1I GCGC 1 cut(s) 2035
HincII GTYRAC 2 cut(s) 2580, 3225
HindII GTYRAC 2 cut(s) 2580, 3225
HindIII AAGCTT 2 cut(s) 1382, 1778
HpaII CCGG 6 cut(s) 32, 284, 574, 748, 1354, 1979
HpyAV CCTTC 6 cut(s) 550, 773, 1215, 1220, 1748, 2786
HpyCH4III ACNGT 9 cut(s) 649, 1095, 1556, 1897, 2114, 2337, 2577, 2837, 3069
HpyCH4IV ACGT 6 cut(s) 1307, 1340, 1877, 2287, 2395, 3263
HpyF10VI GCNNNNNNNGC 8 cut(s) 19, 55, 1680, 2773, 3044, 3097, 3692, 3797
HpySE526I ACGT 6 cut(s) 1307, 1340, 1877, 2287, 2395, 3263
HspAI GCGC 1 cut(s) 2035
Ksp22I TGATCA 1 cut(s) 1723
LmnI GCTCC 3 cut(s) 358, 1704, 3474
Lsp1109I GCAGC 9 cut(s) 100, 1208, 1279, 1843, 1895, 2015, 2290, 2417, 2704
LweI GCATC 8 cut(s) 22, 1007, 2347, 2638, 2737, 3056, 3165, 3384
MaeI CTAG 9 cut(s) 71, 360, 596, 882, 1764, 1917, 1994, 3687, 3871
MaeII ACGT 6 cut(s) 1307, 1340, 1877, 2287, 2395, 3263
MaeIII GTNAC 6 cut(s) 395, 682, 875, 1996, 2736, 3321
MbiI CCGCTC 1 cut(s) 6
MfeI CAATTG 4 cut(s) 1727, 2055, 2448, 2556
MflI RGATCY 5 cut(s) 1583, 1603, 2311, 3301, 3864
MhlI GDGCHC 4 cut(s) 1307, 1812, 3461, 3731
MlsI TGGCCA 2 cut(s) 144, 3351
MluNI TGGCCA 2 cut(s) 144, 3351
MlyI GAGTC 7 cut(s) 75, 403, 761, 932, 2210, 2498, 3424
MmeI TCCRAC 4 cut(s) 234, 2064, 2445, 2989
Mox20I TGGCCA 2 cut(s) 144, 3351
MroXI GAANNNNTTC 2 cut(s) 557, 1361
MscI TGGCCA 2 cut(s) 144, 3351
MslI CAYNNNNRTG 1 cut(s) 2262
Msp20I TGGCCA 2 cut(s) 144, 3351
MspA1I CMGCKG 2 cut(s) 968, 1123
MspI CCGG 6 cut(s) 32, 284, 574, 748, 1354, 1979
MspR9I CCNGG 7 cut(s) 204, 284, 350, 575, 749, 953, 1979
MunI CAATTG 4 cut(s) 1727, 2055, 2448, 2556
Mva1269I GAATGC 3 cut(s) 408, 1667, 2633
MvaI CCWGG 3 cut(s) 204, 350, 953
MvnI CGCG 1 cut(s) 268
MwoI GCNNNNNNNGC 8 cut(s) 19, 55, 1680, 2773, 3044, 3097, 3692, 3797
NciI CCSGG 4 cut(s) 284, 575, 749, 1979
NcoI CCATGG 1 cut(s) 3060
NdeI CATATG 3 cut(s) 1545, 3470, 3595
NlaIV GGNNCC 4 cut(s) 354, 1574, 1706, 1902
NmuCI GTSAC 3 cut(s) 395, 682, 875
NspI RCATGY 1 cut(s) 2350
NspV TTCGAA 1 cut(s) 514
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 1 cut(s) 1527
PctI GAATGC 3 cut(s) 408, 1667, 2633
PdmI GAANNNNTTC 2 cut(s) 557, 1361
PflMI CCANNNNNTGG 1 cut(s) 3642
PleI GAGTC 7 cut(s) 75, 402, 760, 931, 2210, 2497, 3423
PmaCI CACGTG 1 cut(s) 1308
PmlI CACGTG 1 cut(s) 1308
PpsI GAGTC 7 cut(s) 75, 402, 760, 931, 2210, 2497, 3423
Ppu21I YACGTR 2 cut(s) 1308, 2288
Psp124BI GAGCTC 1 cut(s) 1812
Psp6I CCWGG 3 cut(s) 202, 348, 951
PspCI CACGTG 1 cut(s) 1308
PspGI CCWGG 3 cut(s) 202, 348, 951
PspN4I GGNNCC 4 cut(s) 354, 1574, 1706, 1902
PspPI GGNCC 2 cut(s) 1513, 3492
PspXI VCTCGAGB 1 cut(s) 83
PstI CTGCAG 2 cut(s) 2005, 3802
PstNI CAGNNNCTG 1 cut(s) 2009
PsuI RGATCY 5 cut(s) 1583, 1603, 2311, 3301, 3864
PvuII CAGCTG 2 cut(s) 968, 1123
RsaI GTAC 9 cut(s) 298, 527, 1915, 1921, 2290, 2365, 3291, 3435, 3522
RsaNI GTAC 9 cut(s) 297, 526, 1914, 1920, 2289, 2364, 3290, 3434, 3521
RseI CAYNNNNRTG 1 cut(s) 2262
SacI GAGCTC 1 cut(s) 1812
Sau96I GGNCC 2 cut(s) 1513, 3492
ScaI AGTACT 1 cut(s) 1915
SchI GAGTC 7 cut(s) 75, 403, 761, 932, 2210, 2498, 3424
ScrFI CCNGG 7 cut(s) 204, 284, 350, 575, 749, 953, 1979
SduI GDGCHC 4 cut(s) 1307, 1812, 3461, 3731
SfaNI GCATC 8 cut(s) 22, 1007, 2347, 2638, 2737, 3056, 3165, 3384
SfcI CTRYAG 8 cut(s) 222, 969, 1555, 1905, 2001, 2863, 2955, 3798
Sfr274I CTCGAG 1 cut(s) 83
SfuI TTCGAA 1 cut(s) 514
SinI GGWCC 1 cut(s) 3492
SlaI CTCGAG 1 cut(s) 83
SmiMI CAYNNNNRTG 1 cut(s) 2262
SmlI CTYRAG 4 cut(s) 83, 1567, 1834, 2732
SmoI CTYRAG 4 cut(s) 83, 1567, 1834, 2732
SnaBI TACGTA 1 cut(s) 2288
SpeI ACTAGT 2 cut(s) 1916, 1993
SspI AATATT 3 cut(s) 802, 1406, 2270
SspMI CTAG 9 cut(s) 71, 360, 596, 882, 1764, 1917, 1994, 3687, 3871
SstI GAGCTC 1 cut(s) 1812
StyD4I CCNGG 7 cut(s) 202, 282, 348, 573, 747, 951, 1977
StyI CCWWGG 3 cut(s) 145, 446, 3060
TaaI ACNGT 9 cut(s) 649, 1095, 1556, 1897, 2114, 2337, 2577, 2837, 3069
TaiI ACGT 6 cut(s) 1310, 1343, 1880, 2290, 2398, 3266
TaqI TCGA 6 cut(s) 84, 514, 1551, 1800, 2380, 2847
TatI WGTACW 5 cut(s) 525, 1913, 1919, 3289, 3520
TauI GCSGC 2 cut(s) 61, 271
TseFI GTSAC 3 cut(s) 395, 682, 875
TseI GCWGC 9 cut(s) 88, 1196, 1292, 1856, 1883, 2003, 2303, 2405, 2717
Tsp45I GTSAC 3 cut(s) 395, 682, 875
TspGWI ACGGA 3 cut(s) 6, 240, 1148
Van91I CCANNNNNTGG 1 cut(s) 3642
VpaK11BI GGWCC 1 cut(s) 3492
XapI RAATTY 2 cut(s) 2105, 3012
XceI RCATGY 1 cut(s) 2350
XcmI CCANNNNNNNNNTGG 1 cut(s) 3381
XhoI CTCGAG 1 cut(s) 83
XmnI GAANNNNTTC 2 cut(s) 557, 1361
XspI CTAG 9 cut(s) 71, 360, 596, 882, 1764, 1917, 1994, 3687, 3871
ZrmI AGTACT 1 cut(s) 1915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.