MD04G1175700.v1.1

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009696
Physical Location & Seq
Reverse (-)
26678754 .. 26686540
7787 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1175700.v1.1.491

Sequence Viewer

Length: 3861 bp
ATGACTTCTGGGCACCCGTTGCTGTCTTCGTCTGATTCTCCATCAGCGCCACTGGTTCCATCCTCTTCTCTTACTAAGAACCTTGCCCGTATCTCCTCCAATGCTTCATTTTCTTCTTCCAGTCTTGACAACAATGATGATGCTCAAAGTGATTTACTTGAGGTGAAGGACGACGTTGCTGTTTCTGGTTGCTCTGAGAAACCCTTAGAAAATTCCACTACTCTTGCAGGTCCATTCGGTTCTTGGTTATTGCCGCAGTTTCCATTCGAAAACCCCACGCGGGATAGAAGACGCCTGGTGTCGCGGGGTGCCATGGAACTGCATAATGAAAATAGAAATTCAGGAACTCTTGAAATCTCCCAGGGTTCATCTAGGGTTCAGGAAAAGTTGTCTCAGAGGATCCGTCACAAAAGTGTGCAGTTCGATGATAACTTGCTGCATGATGACAATCCAAGGTTGATCTATATTAACGATCCGAAGAGGACAAATGACAAATATGAGTTCACTGGGAATGAGATTCGAACTAGCAAGTACACCATCATTACCTTCTTGCCCAAGAATCTTTTCATTCAGTTTCATCGGGTTGCTTATTTGTATTTTCTAGCTATTGCTGCCCTCAACCAGCTTCCACCTCTTGCAGTCTTTGGAAGAACAGTGTCTCTTTTCCCCCTTCTGTTTGTGCTCTGTGTCACAGCTATCAAAGATGGCTATGAAGATTGGCGGAGACATAGATCAGACAGGAATGAGAATAACCGGGAGGCTTTGGTGTTTCAATCTGGCCAATTTCAACTGAAGAAATGGAAACATATTCAAGTGGGCGAGGTTCTAAAGATTTGTGCCGATGACACAATTCCTTGTGATGTGGTCTTGTTAGGGACAAGCGACCCTAGTGGAATTGCCTACATTCAAACAATGAATTTGGATGGTGAGTCGAACTTGAAAACAAGGTATGCGCGGCAGGAAACAACTTCAGCAGTATGTGAAGGGTGTACATTTTTAGGGCTCATCAGATGTGAACAACCTAATAGGAATATCTATGAGTTCACTGCCAACATGGAGTTTAATGGGCATAAATTTCCCCTGAGTCAATCAAATATAGTTTTGCGTGGTTGCCAGCTGAAGAACACAGCCTGGGCAATTGGTGTCGTGGTATATGCTGGACAGGAAACCAAGGCAATGTTGAATAGTGCAGCTTCTCCTTCCAAGAGAAGTAAACTGGAAAGCTACATGAATAGGGAAACTCTCTGGCTGTCGGTTTTCCTTTTTGTTATGTGTGCAGTTGTGGCCACTGGCATGGGCCTGTGGCTCATGCACCATAAAGGTCAGATTGATACCTTGGCTTATTACCGGAAAAGATACTACTCTGATGGGAAAGAGAATGGAAAAACCTATAGGTTTTATGGGATACCTATGGAGATCTTTTTCTCCTTTTTGAGTTCTATCATAGTTTTCCAGATAATGATACCAATCTCTCTTTATATTACAATGGAGTTGGTTCGATTGGGCCAGTCATATTTCATGATCGAAGACAGGCATATGTTTGACAGTAGCTCTGGCTCAAGGTTCCAGTGCAGATCGTTGAATATCAACGAGGATTTGGGTCAAATACGATATATTTTTTCAGACAAAACAGGGACACTTACCGAGAACAAAATGGAATTCCGAAGAGCAAGCATATTTGGGAGGAGTTTTGGGACCTCTTTGCAGGAAGCAAATGTTGCAGGAATAGGATTAGGTAGAAAGAGATGGAAGCTCAAAAGCGAAATTTCTGTAGATAATGAGCTTGTGGAATTTTTGCACAAAGACTTCAGTGAAAATGACAGGATTGCCGCGCATGAGTTTTTTCTTACATTGGCTGCTTGCAATACTGTGGTTCCTATTGTCAGTAATAGTACATCTTCCAGTTGCGGAAAAAGTGAATTAGATGATGTAGAAGCTATTGACTATCAGGGGGAATCTCCTGATGAGCAAGCATTAGTTTCTGCAGCCTCTGCATATGGATATACGCTTTTTGAGCGCACATCTGGGCACATTGTTATGGATGTCAATGGTGAGAAACTAAGGTTGGATGTATTGGGTCTGCATGAGTTTGATAGCGTGCGAAAAAGGATGTCCGTTGTTATCAGATTTCCAAACAATACTGTAAAGGTTTTGGTGAAAGGTGCTGACACTACTATGTTCGGCACTTTAGCAAATGACTCTGAAAGGGATGATCATCTGACTCTTTCAACTCAAAGCCATCTGAGTGAATATTCCTCAGAAGGTTTACGTACTCTTGTAGTTGCTGCCAGGGATCTTACAGATGAACAACTTGAGCAGTGGCAAAGCATGTATGAAGATGCAAGTACCTCGTTGACTGATCGGTCCTTGAAATTACGTCAAACAGCAGCTCTCATTGAATGCAACTTAAAGCTTCTTGGGGCGACTGCGATTGAGGATAAGTTACAAGATGGTGTGCCGGAAGCTATTGAGTCTCTCCGGCAAGCAGGGATCAAGGTTTGGGTTCTTACTGGAGATAAGCAAGAGACAGCTATTTCGATTGGTCTATCATGCAAACTCTTGACAGCAGATATGCAACAAATTATTATTAATGGAACTTCTGAGGATGAATGCCGAAATCTTTTGGCTGATTCTATGGCAAAATATGGTGTAAAATCGTCCAATAAAAGAGACCCAAGTTTCAAACTGAAAAAAAATGCTGAAAATGGCTACCTTGAGATACCTGGCAATGCAAAGACATCCAGTGTGCCTGAATGGAATGGAAGGAAGGAAGAAGGAAAAATGAACGCACCATTAGCACTCATAATAGATGGGAACAGCTTGGTATACATTCTGGAGAAAGATCTGGAGTTAGAGCTGTTCGACCTTGCCACATCCTGTAGTGTTGTGTTATGCTGTCGTGTTGCACCTCTGCAGAAAGCTGGAATTGTTGATCTGATTAAGACTCGTACTGATGACATGACATTGGCTATAGGTGATGGGGCAAATGATGTTTCAATGATCCAAATGGCGGATGTTGGAGTTGGAATTTGTGGTCAGGAAGGACGTCAAGCTGTGATGGCTTCAGACTTTGCTATGGGACAGTTTCGGTTTTTGAAAACATTACTTTTGGTGCATGGGCACTGGAATTATCAGCGTGTTGGCTATATGATTCTGTACAACTTCTACCGCAATGCAGTTTTTGTACTGATGCTATTTTGGTTTATATTATCCACTGCTTTTTCAACAACTTCCGCTTTAACAGATTGGAGTAGTGTTTTCTATTCTGTCATTTATACTTCACTCCCCACAATTGTTGTTGGTATACTGGACAAAGACTTGAGCCACAGGACACTGTTACAATATCCAAAACTCTATGGTGCTGGCCATAGACACGAGGCATACAATTTACATCTCTTCTGGATCACAATGCTTGACACCGTATGGCAGAGTCTTGTTCTCTTCTATGTACCCCTCTTCACCTATAAGGATAGCTCAATAGACATATGGAGCATGGGCAGTTTATGGACAATTGCAGTTGTTGTCCTAGTCAATGTACATTTGGCAATGGACGTTCATCGTTGGGTATTCATCACTCAAATCGCAGTATGGGGTTCAATAATTATCACATATGCCTGTATGGTGGTATTGGATTCTATACCTGTCTTTCCTAATTACTGGACTATATACCATTTGGCAAAGTCTCCCACATATTGGATCGCCATTTTGCTTATAACTGTTGTCGCGTTGCTCCCTCGCTTTGTGTTCAAAGTTGTAAATCATATCTTTTGGCCTTCGGATATCCAGATAGCTAGGGAAGCCGAGGTTTTAAATAGGCAACGTAAACATTTGAGCTCAAAGCAAGATGACAGTTCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1287

Amino Acids

144.83

Weight (kDa)

6.15

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhoLip_ATPase_N PF16209 154 - 219 2.3e-23 Phospholipid-translocating ATPase N-terminal
Cation_ATPase PF13246 652 - 816 5e-12 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 784 - 848 8.5e-06 haloacid dehalogenase-like hydrolase
PhoLip_ATPase_C PF16212 1019 - 1259 3.4e-78 Phospholipid-translocating P-type ATPase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3713
AasI GACNNNNNNGTC 1 cut(s) 2362
AatII GACGTC 1 cut(s) 3049
Acc36I ACCTGC 1 cut(s) 218
AccB1I GGYRCC 2 cut(s) 12, 308
AccB7I CCANNNNNTGG 1 cut(s) 3693
AccI GTMKAC 2 cut(s) 2826, 3304
AccII CGCG 5 cut(s) 280, 304, 955, 1832, 3725
AclWI GGATC 8 cut(s) 394, 407, 467, 2301, 2498, 2995, 3410, 3704
AcoI YGGCCR 3 cut(s) 778, 1284, 3364
AcsI RAATTY 8 cut(s) 211, 337, 916, 1073, 1658, 1764, 1790, 3027
AcuI CTGAAG 5 cut(s) 812, 954, 1139, 1792, 3048
AcyI GRCGYC 2 cut(s) 292, 3046
AfiI CCNNNNNNNGG 3 cut(s) 280, 3010, 3693
AjnI CCWGG 5 cut(s) 294, 360, 1130, 2288, 2722
AjuI GAANNNNNNNTTGG 4 cut(s) 548, 580, 2048, 2080
AleI CACNNNNGTG 1 cut(s) 411
Alw21I GWGCWC 2 cut(s) 684, 3836
Alw26I GTCTC 7 cut(s) 396, 663, 718, 2478, 2519, 2664, 3687
AlwI GGATC 8 cut(s) 394, 407, 467, 2301, 2498, 2995, 3410, 3704
AlwNI CAGNNNCTG 1 cut(s) 1991
AoxI GGCC 6 cut(s) 778, 1284, 1297, 1504, 3364, 3770
ApeKI GCWGC 7 cut(s) 436, 611, 1190, 1856, 1985, 2285, 2387
ApoI RAATTY 8 cut(s) 211, 337, 916, 1073, 1658, 1764, 1790, 3027
ArsI GACNNNNNNTTYG 2 cut(s) 2518, 2550
AseI ATTAAT 1 cut(s) 2589
Asp700I GAANNNNTTC 1 cut(s) 563
AspLEI GCGC 4 cut(s) 49, 955, 1834, 2019
AspS9I GGNCC 5 cut(s) 230, 1297, 1504, 1695, 2364
AsuC2I CCSGG 1 cut(s) 755
AsuHPI GGTGA 6 cut(s) 175, 938, 2063, 2167, 2987, 3451
AsuII TTCGAA 2 cut(s) 267, 520
AvaII GGWCC 3 cut(s) 230, 1695, 2364
BaeGI GKGCMC 3 cut(s) 15, 2031, 3123
BalI TGGCCA 3 cut(s) 780, 1286, 3366
BamHI GGATCC 1 cut(s) 399
BanI GGYRCC 2 cut(s) 12, 308
BanII GRGCYC 2 cut(s) 1005, 3836
BauI CACGAG 1 cut(s) 3374
BbsI GAAGAC 3 cut(s) 18, 295, 1533
Bbv12I GWGCWC 2 cut(s) 684, 3836
BbvI GCAGC 7 cut(s) 423, 598, 1202, 1843, 1997, 2272, 2399
BcgI CGANNNNNNTGC 2 cut(s) 2331, 2365
BciT130I CCWGG 5 cut(s) 296, 362, 1132, 2290, 2724
BciVI GTATCC 1 cut(s) 1398
BclI TGATCA 1 cut(s) 2212
BcnI CCSGG 1 cut(s) 755
BcoDI GTCTC 7 cut(s) 396, 663, 718, 2478, 2519, 2664, 3687
BfaI CTAG 6 cut(s) 372, 525, 602, 888, 3527, 3792
BfmI CTRYAG 6 cut(s) 1390, 1770, 1983, 2878, 2912, 2970
BfoI RGCGCY 1 cut(s) 50
BfuAI ACCTGC 1 cut(s) 218
BfuI GTATCC 1 cut(s) 1398
BglII AGATCT 2 cut(s) 1416, 2842
Bme1390I CCNGG 6 cut(s) 296, 362, 755, 1132, 2290, 2724
Bme18I GGWCC 3 cut(s) 230, 1695, 2364
BmgT120I GGNCC 5 cut(s) 230, 1297, 1504, 1695, 2364
BmiI GGNNCC 7 cut(s) 14, 57, 310, 401, 1565, 1696, 1875
BmrFI CCNGG 6 cut(s) 296, 362, 755, 1132, 2290, 2724
BmrI ACTGGG 1 cut(s) 516
BmsI GCATC 3 cut(s) 130, 2329, 3180
BmuI ACTGGG 1 cut(s) 516
BpiI GAAGAC 3 cut(s) 18, 295, 1533
BpmI CTGGAG 3 cut(s) 2532, 2855, 2867
Bpu14I TTCGAA 2 cut(s) 267, 520
BpuEI CTTGAG 5 cut(s) 179, 1543, 2333, 2735, 3340
BpuMI CCSGG 1 cut(s) 755
BsaAI YACGTR 1 cut(s) 2270
BsaBI GATNNNNATC 3 cut(s) 447, 1466, 2214
BsaHI GRCGYC 2 cut(s) 292, 3046
BsaI GGTCTC 1 cut(s) 2664
BsaJI CCNNGG 9 cut(s) 312, 360, 361, 452, 1131, 1170, 1335, 2289, 3801
BsaWI WCCGGW 1 cut(s) 1347
Bsc4I CCNNNNNNNGG 3 cut(s) 280, 3010, 3693
Bse3DI GCAATG 4 cut(s) 1182, 2734, 3178, 3552
Bse8I GATNNNNATC 3 cut(s) 447, 1466, 2214
BseBI CCWGG 5 cut(s) 296, 362, 1132, 2290, 2724
BseDI CCNNGG 9 cut(s) 312, 360, 361, 452, 1131, 1170, 1335, 2289, 3801
BseJI GATNNNNATC 3 cut(s) 447, 1466, 2214
BseLI CCNNNNNNNGG 3 cut(s) 280, 3010, 3693
BseMI GCAATG 4 cut(s) 1182, 2734, 3178, 3552
BseMII CTCAG 6 cut(s) 186, 407, 1073, 2234, 2271, 2592
BseRI GAGGAG 2 cut(s) 85, 1699
BseSI GKGCMC 3 cut(s) 15, 2031, 3123
BseXI GCAGC 7 cut(s) 423, 598, 1202, 1843, 1997, 2272, 2399
BsgI GTGCAG 4 cut(s) 437, 1209, 1296, 1591
Bsh1236I CGCG 5 cut(s) 280, 304, 955, 1832, 3725
BshFI GGCC 6 cut(s) 780, 1286, 1299, 1506, 3366, 3772
BshNI GGYRCC 2 cut(s) 12, 308
BsiHKAI GWGCWC 2 cut(s) 684, 3836
BsiSI CCGG 4 cut(s) 754, 1348, 2459, 2479
BslFI GGGAC 4 cut(s) 889, 1648, 1708, 3093
BslI CCNNNNNNNGG 3 cut(s) 280, 3010, 3693
BsmAI GTCTC 7 cut(s) 396, 663, 718, 2478, 2519, 2664, 3687
BsmFI GGGAC 4 cut(s) 889, 1648, 1708, 3093
BsmI GAATGC 2 cut(s) 2405, 2615
BsnI GGCC 6 cut(s) 780, 1286, 1299, 1506, 3366, 3772
Bso31I GGTCTC 1 cut(s) 2664
Bsp119I TTCGAA 2 cut(s) 267, 520
Bsp1286I GDGCHC 6 cut(s) 15, 684, 1005, 2031, 3123, 3836
Bsp1407I TGTACA 3 cut(s) 989, 3156, 3535
Bsp19I CCATGG 1 cut(s) 312
BspANI GGCC 6 cut(s) 780, 1286, 1299, 1506, 3366, 3772
BspCNI CTCAG 6 cut(s) 187, 406, 1074, 2235, 2270, 2593
BspFNI CGCG 5 cut(s) 280, 304, 955, 1832, 3725
BspHI TCATGA 1 cut(s) 1518
BspLI GGNNCC 7 cut(s) 14, 57, 310, 401, 1565, 1696, 1875
BspMAI CTGCAG 2 cut(s) 1987, 2916
BspMI ACCTGC 1 cut(s) 218
BspPI GGATC 8 cut(s) 394, 407, 467, 2301, 2498, 2995, 3410, 3704
BspQI GCTCTTC 1 cut(s) 1660
BspT104I TTCGAA 2 cut(s) 267, 520
BspT107I GGYRCC 2 cut(s) 12, 308
BspTNI GGTCTC 1 cut(s) 2664
BsrDI GCAATG 4 cut(s) 1182, 2734, 3178, 3552
BsrGI TGTACA 3 cut(s) 989, 3156, 3535
BssECI CCNNGG 9 cut(s) 312, 360, 361, 452, 1131, 1170, 1335, 2289, 3801
BssNAI GTATAC 2 cut(s) 2827, 3305
BssNI GRCGYC 2 cut(s) 292, 3046
BssSI CACGAG 1 cut(s) 3374
BssT1I CCWWGG 4 cut(s) 312, 452, 1170, 1335
Bst1107I GTATAC 2 cut(s) 2827, 3305
Bst2BI CACGAG 1 cut(s) 3374
Bst2UI CCWGG 5 cut(s) 296, 362, 1132, 2290, 2724
Bst4CI ACNGT 9 cut(s) 655, 1547, 1870, 2143, 3084, 3336, 3421, 3718, 3851
Bst6I CTCTTC 6 cut(s) 70, 473, 1660, 3401, 3446, 3461
BstACI GRCGYC 2 cut(s) 292, 3046
BstAPI GCANNNNNTGC 3 cut(s) 19, 1718, 1991
BstAUI TGTACA 3 cut(s) 989, 3156, 3535
BstBAI YACGTR 1 cut(s) 2270
BstBI TTCGAA 2 cut(s) 267, 520
BstC8I GCNNGC 7 cut(s) 1115, 1672, 1861, 1971, 2099, 2484, 3364
BstDEI CTNAG 9 cut(s) 75, 195, 205, 393, 1082, 2060, 2243, 2257, 2601
BstDSI CCRYGG 1 cut(s) 312
BstFNI CGCG 5 cut(s) 280, 304, 955, 1832, 3725
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 4 cut(s) 49, 955, 1834, 2019
BstMAI GTCTC 7 cut(s) 396, 663, 718, 2478, 2519, 2664, 3687
BstMWI GCNNNNNNNGC 9 cut(s) 19, 611, 1283, 1718, 1991, 2014, 2795, 3059, 3797
BstNI CCWGG 5 cut(s) 296, 362, 1132, 2290, 2724
BstNSI RCATGY 1 cut(s) 2332
BstSCI CCNGG 6 cut(s) 294, 360, 753, 1130, 2288, 2722
BstSFI CTRYAG 6 cut(s) 1390, 1770, 1983, 2878, 2912, 2970
BstSLI GKGCMC 3 cut(s) 15, 2031, 3123
BstSNI TACGTA 1 cut(s) 2270
BstUI CGCG 5 cut(s) 280, 304, 955, 1832, 3725
BstV1I GCAGC 7 cut(s) 423, 598, 1202, 1843, 1997, 2272, 2399
BstV2I GAAGAC 3 cut(s) 18, 295, 1533
BstX2I RGATCY 4 cut(s) 399, 1416, 2293, 2842
BstXI CCANNNNNNTGG 1 cut(s) 1294
BstYI RGATCY 4 cut(s) 399, 1416, 2293, 2842
BstZ17I GTATAC 2 cut(s) 2827, 3305
BsuI GTATCC 1 cut(s) 1398
BsuRI GGCC 6 cut(s) 780, 1286, 1299, 1506, 3366, 3772
BtgI CCRYGG 1 cut(s) 312
BtsI GCAGTG 3 cut(s) 1044, 2324, 3213
BveI ACCTGC 1 cut(s) 218
Cac8I GCNNGC 7 cut(s) 1115, 1672, 1861, 1971, 2099, 2484, 3364
CaiI CAGNNNCTG 1 cut(s) 1991
CciI TCATGA 1 cut(s) 1518
CfoI GCGC 4 cut(s) 49, 955, 1834, 2019
Cfr13I GGNCC 5 cut(s) 230, 1297, 1504, 1695, 2364
CseI GACGC 1 cut(s) 300
CspCI CAANNNNNGTGG 4 cut(s) 205, 240, 3277, 3312
DdeI CTNAG 9 cut(s) 75, 195, 205, 393, 1082, 2060, 2243, 2257, 2601
DraI TTTAAA 1 cut(s) 3810
DrdI GACNNNNNNGTC 1 cut(s) 2362
DseDI GACNNNNNNGTC 1 cut(s) 2362
EaeI YGGCCR 3 cut(s) 778, 1284, 3364
Eam1104I CTCTTC 6 cut(s) 70, 473, 1660, 3401, 3446, 3461
EarI CTCTTC 6 cut(s) 70, 473, 1660, 3401, 3446, 3461
EciI GGCGGA 2 cut(s) 736, 3026
Ecl136II GAGCTC 1 cut(s) 3834
Eco105I TACGTA 1 cut(s) 2270
Eco130I CCWWGG 4 cut(s) 312, 452, 1170, 1335
Eco24I GRGCYC 2 cut(s) 1005, 3836
Eco31I GGTCTC 1 cut(s) 2664
Eco32I GATATC 1 cut(s) 3781
Eco47I GGWCC 3 cut(s) 230, 1695, 2364
Eco53kI GAGCTC 1 cut(s) 3834
Eco57I CTGAAG 5 cut(s) 812, 954, 1139, 1792, 3048
EcoICRI GAGCTC 1 cut(s) 3834
EcoO109I RGGNCCY 1 cut(s) 1695
EcoRI GAATTC 1 cut(s) 1658
EcoRII CCWGG 5 cut(s) 294, 360, 1130, 2288, 2722
EcoRV GATATC 1 cut(s) 3781
EcoT14I CCWWGG 4 cut(s) 312, 452, 1170, 1335
EcoT38I GRGCYC 2 cut(s) 1005, 3836
ErhI CCWWGG 4 cut(s) 312, 452, 1170, 1335
FaqI GGGAC 4 cut(s) 889, 1648, 1708, 3093
FauI CCCGC 2 cut(s) 273, 297
FauNDI CATATG 4 cut(s) 1536, 1996, 3485, 3610
FbaI TGATCA 1 cut(s) 2212
FblI GTMKAC 2 cut(s) 2826, 3304
FriOI GRGCYC 2 cut(s) 1005, 3836
FspBI CTAG 6 cut(s) 372, 525, 602, 888, 3527, 3792
GlaI GCGC 4 cut(s) 48, 954, 1833, 2018
GsuI CTGGAG 3 cut(s) 2532, 2855, 2867
HaeII RGCGCY 1 cut(s) 50
HaeIII GGCC 6 cut(s) 780, 1286, 1299, 1506, 3366, 3772
HapII CCGG 4 cut(s) 754, 1348, 2459, 2479
HgaI GACGC 1 cut(s) 300
HhaI GCGC 4 cut(s) 49, 955, 1834, 2019
Hin1I GRCGYC 2 cut(s) 292, 3046
Hin6I GCGC 4 cut(s) 47, 953, 1832, 2017
HinP1I GCGC 4 cut(s) 47, 953, 1832, 2017
HincII GTYRAC 1 cut(s) 2355
HindII GTYRAC 1 cut(s) 2355
HindIII AAGCTT 1 cut(s) 2411
HpaII CCGG 4 cut(s) 754, 1348, 2459, 2479
HphI GGTGA 6 cut(s) 175, 938, 2063, 2167, 2987, 3451
Hpy99I CGWCG 1 cut(s) 176
HpyCH4III ACNGT 9 cut(s) 655, 1547, 1870, 2143, 3084, 3336, 3421, 3718, 3851
HpyCH4IV ACGT 6 cut(s) 174, 2269, 2377, 3046, 3552, 3820
HpyF10VI GCNNNNNNNGC 9 cut(s) 19, 611, 1283, 1718, 1991, 2014, 2795, 3059, 3797
HpyF3I CTNAG 9 cut(s) 75, 195, 205, 393, 1082, 2060, 2243, 2257, 2601
HpySE526I ACGT 6 cut(s) 174, 2269, 2377, 3046, 3552, 3820
Hsp92I GRCGYC 2 cut(s) 292, 3046
HspAI GCGC 4 cut(s) 47, 953, 1832, 2017
Ksp22I TGATCA 1 cut(s) 2212
LguI GCTCTTC 1 cut(s) 1660
LmnI GCTCC 2 cut(s) 3489, 3735
Lsp1109I GCAGC 7 cut(s) 423, 598, 1202, 1843, 1997, 2272, 2399
LweI GCATC 3 cut(s) 130, 2329, 3180
MaeI CTAG 6 cut(s) 372, 525, 602, 888, 3527, 3792
MaeII ACGT 6 cut(s) 174, 2269, 2377, 3046, 3552, 3820
MaeIII GTNAC 4 cut(s) 404, 688, 2442, 3336
MfeI CAATTG 3 cut(s) 1137, 3291, 3510
MflI RGATCY 4 cut(s) 399, 1416, 2293, 2842
MhlI GDGCHC 6 cut(s) 15, 684, 1005, 2031, 3123, 3836
MlsI TGGCCA 3 cut(s) 780, 1286, 3366
MluNI TGGCCA 3 cut(s) 780, 1286, 3366
MlyI GAGTC 7 cut(s) 938, 1093, 2192, 2215, 2480, 2938, 3439
MmeI TCCRAC 3 cut(s) 2046, 2998, 3004
Mox20I TGGCCA 3 cut(s) 780, 1286, 3366
MroXI GAANNNNTTC 1 cut(s) 563
MscI TGGCCA 3 cut(s) 780, 1286, 3366
MseI TTAA 7 cut(s) 468, 1062, 2408, 2589, 2940, 3239, 3809
MslI CAYNNNNRTG 5 cut(s) 411, 1292, 2036, 2174, 2244
Msp20I TGGCCA 3 cut(s) 780, 1286, 3366
MspA1I CMGCKG 1 cut(s) 1117
MspI CCGG 4 cut(s) 754, 1348, 2459, 2479
MspR9I CCNGG 6 cut(s) 296, 362, 755, 1132, 2290, 2724
MunI CAATTG 3 cut(s) 1137, 3291, 3510
Mva1269I GAATGC 2 cut(s) 2405, 2615
MvaI CCWGG 5 cut(s) 296, 362, 1132, 2290, 2724
MvnI CGCG 5 cut(s) 280, 304, 955, 1832, 3725
MwoI GCNNNNNNNGC 9 cut(s) 19, 611, 1283, 1718, 1991, 2014, 2795, 3059, 3797
NciI CCSGG 1 cut(s) 755
NcoI CCATGG 1 cut(s) 312
NdeI CATATG 4 cut(s) 1536, 1996, 3485, 3610
NlaIV GGNNCC 7 cut(s) 14, 57, 310, 401, 1565, 1696, 1875
NmeAIII GCCGAG 1 cut(s) 3826
NmuCI GTSAC 2 cut(s) 404, 688
NspI RCATGY 1 cut(s) 2332
NspV TTCGAA 2 cut(s) 267, 520
OliI CACNNNNGTG 1 cut(s) 411
PagI TCATGA 1 cut(s) 1518
PasI CCCWGGG 1 cut(s) 361
PciSI GCTCTTC 1 cut(s) 1660
PctI GAATGC 2 cut(s) 2405, 2615
PdmI GAANNNNTTC 1 cut(s) 563
PfeI GAWTC 7 cut(s) 35, 517, 559, 1955, 2630, 3151, 3632
PflMI CCANNNNNTGG 1 cut(s) 3693
PleI GAGTC 7 cut(s) 937, 1092, 2192, 2215, 2479, 2938, 3438
PpsI GAGTC 7 cut(s) 937, 1092, 2192, 2215, 2479, 2938, 3438
Ppu21I YACGTR 1 cut(s) 2270
PpuMI RGGWCCY 1 cut(s) 1695
PshBI ATTAAT 1 cut(s) 2589
PsiI TTATAA 1 cut(s) 3713
Psp124BI GAGCTC 1 cut(s) 3836
Psp5II RGGWCCY 1 cut(s) 1695
Psp6I CCWGG 5 cut(s) 294, 360, 1130, 2288, 2722
PspGI CCWGG 5 cut(s) 294, 360, 1130, 2288, 2722
PspN4I GGNNCC 7 cut(s) 14, 57, 310, 401, 1565, 1696, 1875
PspPI GGNCC 5 cut(s) 230, 1297, 1504, 1695, 2364
PspPPI RGGWCCY 1 cut(s) 1695
PstI CTGCAG 2 cut(s) 1987, 2916
PstNI CAGNNNCTG 1 cut(s) 1991
PsuI RGATCY 4 cut(s) 399, 1416, 2293, 2842
PvuII CAGCTG 1 cut(s) 1117
RseI CAYNNNNRTG 5 cut(s) 411, 1292, 2036, 2174, 2244
SacI GAGCTC 1 cut(s) 3836
SapI GCTCTTC 1 cut(s) 1660
SaqAI TTAA 7 cut(s) 468, 1062, 2408, 2589, 2940, 3239, 3809
Sau96I GGNCC 5 cut(s) 230, 1297, 1504, 1695, 2364
SchI GAGTC 7 cut(s) 938, 1093, 2192, 2215, 2480, 2938, 3439
ScrFI CCNGG 6 cut(s) 296, 362, 755, 1132, 2290, 2724
SduI GDGCHC 6 cut(s) 15, 684, 1005, 2031, 3123, 3836
SfaNI GCATC 3 cut(s) 130, 2329, 3180
SfcI CTRYAG 6 cut(s) 1390, 1770, 1983, 2878, 2912, 2970
SfuI TTCGAA 2 cut(s) 267, 520
SinI GGWCC 3 cut(s) 230, 1695, 2364
SmiMI CAYNNNNRTG 5 cut(s) 411, 1292, 2036, 2174, 2244
SmlI CTYRAG 5 cut(s) 158, 1558, 2312, 2714, 3319
SmoI CTYRAG 5 cut(s) 158, 1558, 2312, 2714, 3319
SnaBI TACGTA 1 cut(s) 2270
SspI AATATT 1 cut(s) 2252
SspMI CTAG 6 cut(s) 372, 525, 602, 888, 3527, 3792
SstI GAGCTC 1 cut(s) 3836
StyD4I CCNGG 6 cut(s) 294, 360, 753, 1130, 2288, 2722
StyI CCWWGG 4 cut(s) 312, 452, 1170, 1335
TaaI ACNGT 9 cut(s) 655, 1547, 1870, 2143, 3084, 3336, 3421, 3718, 3851
TaiI ACGT 6 cut(s) 177, 2272, 2380, 3049, 3555, 3823
TaqI TCGA 8 cut(s) 267, 423, 520, 932, 1498, 1524, 2537, 2862
TaqII GACCGA 1 cut(s) 2352
TatI WGTACW 6 cut(s) 531, 989, 1892, 3156, 3184, 3535
TauI GCSGC 3 cut(s) 256, 958, 1832
TfiI GAWTC 7 cut(s) 35, 517, 559, 1955, 2630, 3151, 3632
Tru1I TTAA 7 cut(s) 468, 1062, 2408, 2589, 2940, 3239, 3809
Tru9I TTAA 7 cut(s) 468, 1062, 2408, 2589, 2940, 3239, 3809
TseFI GTSAC 2 cut(s) 404, 688
TseI GCWGC 7 cut(s) 436, 611, 1190, 1856, 1985, 2285, 2387
Tsp45I GTSAC 2 cut(s) 404, 688
TspGWI ACGGA 2 cut(s) 392, 2104
Van91I CCANNNNNTGG 1 cut(s) 3693
VpaK11BI GGWCC 3 cut(s) 230, 1695, 2364
VspI ATTAAT 1 cut(s) 2589
XapI RAATTY 8 cut(s) 211, 337, 916, 1073, 1658, 1764, 1790, 3027
XceI RCATGY 1 cut(s) 2332
XcmI CCANNNNNNNNNTGG 1 cut(s) 240
XmiI GTMKAC 2 cut(s) 2826, 3304
XmnI GAANNNNTTC 1 cut(s) 563
XspI CTAG 6 cut(s) 372, 525, 602, 888, 3527, 3792
ZraI GACGTC 1 cut(s) 3047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.