MD12G1190000.v1.1

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009696
Physical Location & Seq
Reverse (-)
27232404 .. 27239751
7348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1190000.v1.1.491

Sequence Viewer

Length: 3549 bp
ATGGAACTGCATAATGAAAATAGAAATTCAGGAACTCTTGAAATCTCCCAGGGTTCATCTAGGGTTCAGGAAAAGTTGTCTCAGAGGATCCGTCACAAAAGTGTGCAGTTCGATGATAACTTGCTGCACGATGACAATCCAAGGTTGATCTATATTAACGATCCGAAGAGGACAAATGACAAGTATGAGTTCACTGGGAATGAGATTCGAACTAGCAAGTACACTATCATTACCTTCTTGCCCAAGAATCTTTTCATTCAGTTTCATCGGGTTGCTTATTTGTATTTTCTAGCAATTGCTGCCCTCAACCAGCTTCCACCTCTTGCCGTCTTTGGAAGAACAGTGTCTCTTTTTCCCCTTCTGTTTGTCCTCTTGGTCACAGCTATCAAAGATGGCTATGAAGATTGGCGGAGACATAGATCAGACAGGAATGAGAATAACCGGGAAGCTCTGGTTTTTCAATCTGGCCAATTTCGACCGAAGAAATGGAAACATATTCAAGTGGGCGAGGTTCTGAAGATTTGTGCTGATGACACAATTCCTTGTGACGTGGTTTTGTTAGGGACAAGCGATCCTAGTGGAATTGCCTACATTCAAACAATGAATTTGGATGGTGAGTCGAACTTGAAAACAAGGTATGCTCGGCAGGAAACAACTTCAACAGTATGTGAAGGGTGTACATTTTCAGGGCTCATCAGATGTGAACAACCTAATAGGAACATCTATGAGTTCACTGCCAACATGGAGTTTAATGGGCATAAATTTCCCCTGAGCCAATCAAATATAGTTTTGCGTGGTTGCCAGCTGAAGAACACAGACTGGGCAGTTGGTGTTGCGGTATATGCTGGACAGGAAACCAAGGCAATGCTGAATAGTGCAGCTTCTCCTTCCAAGAGAAGCAAACTGGAAAGCTACATGAACAGGGAAACTCTTTGGCTATCGATTTTCCTTTTTGTTATGTGTGCAGTTGTGGCCACTGGCATGGGCCTGTGGCTCATGCGCCATAAAGGTCAGATTGATACCTTGGCTTATTACCGGAGAAGATTCTACTCATATGGGAATGTGAATGTGAATGGAAAAACCTATAGATTTTATGGGATACCTATGGAGATCTTTTTCTCCTTTTTGAGTTCTATCATAGTTTTCCAGATAATGATACCAATCTCTCTGTATATTACAATGGAGTTGGTTCGATTGGGCCAGTCCTATTTCATGATTGAAGACAGGCATATGTTTGACAGTAGCTCTGGCTCAAGATTCCAGTGCAGATCTTTGAATATCAATGAGGATTTGGGTCAAATACGATATATTTTTTCAGACAAAACAGGGACACTTACCGAGAACAAAATGGAATTCCGAAGAGCAAGCATATTTGGGAGGAATTTTGGGACATCTTTGCAGGAAGCAAATGTTGCAGGAATCGGTTTAGGTAGAAAGAGATGGAAGCTCAAAAGCGAAATTTCTGTAGATAATGAGCTCATGGAGTTGTTGCACAAAGACCTAGGTGGAGATGACAGGATTGCTGCGCATGAGTTTTTCCTTACGTTGGCTGCTTGCAACACTGTGGTTCCTATTGTCAGTAATGGTACATCTTCCAGTTGCGGAAAAAGTGAATTGGATGATGTAGAAGCTATTGACTATCAGGGGGAATCTCCCGATGAGCAAGCATTAGTTTCTGCAGCCTCTGCATATGGATATACACTTTTTGAGCGCACATCTGGGCATATTGTTATGGATGTCAATGGTGAGAAACTAAGGTTGGATGTATTGGGACTTCATGAGTTTGATAGCGTGCGAAAAAGGATGTCCGTTGTTATCAGATTTCCAAACAATAGTGTAAAGGTGTTGGTGAAAGGTGCTGATACTACAATGCTCAACACTTTAGCAAATGACTCTGAAAGGGATGATCATGTGACGCGTTTAACTCAGAATCATCTGAGTGAATATTCGTCAGAAGGTTTACGTACTCTTGTAGTTGCTTCCAGGGATCTTACAGATGAAGAACTTGAGCAGTGGCAAAGCATGTATGAAGATGCAAGTACCTCGTTGACTGATCGGTCCTCGAAATTACGTCAAACAGCAGGTCTCATAGAATGCAACTTAAAGCTTCTTGGGGCGACTGCAATTGAGGATAAGCTACAAGATGGTGTGCCAGAAGCTATTGAGTCCCTCCGGCAAGCAGGAATCAAGGTTTGGGTTCTGACTGGAGATAAGCAAGAGACAGCTATTTCGATTGGTATATCATGCAAACTCTTGACAGCAGATATGCAACAAATTATTATAAATGGAACTTCTGAGGATGAATGCCGAAATCTTTTGGCCGTTTCTATGGAAAAATATGGTGTAAAATCATCCAATAAAATTGACCCGAGTTTCAAACTGAAAAAAAATGCTGAAAATGGCTATCTTGAGATACCTGGCAATGAAAAGACATCCAGTGTGCCCCAATGGAATGGAGGGAAGGAAGAAGGAAAGATGAATGCACCATTAGCACTCATAATAGATGGGAACAGTTTGGTATACATTCTGGAGAAAGATCTGGAGTCAGAGCTATTCAACCTTGCCACTTCCTGTAGTGTTGTGTTATGCTGTCGTGTTGCACCTTTGCAGAAAGCTGGAATTGTTGATCTAATTAAGACCCGTACTGATGATATGACACTGGCTATAGGTGATGGAGCAAATGATGTTTCAATGATCCAAACGGCGGATGTTGGAGTTGGAATTTGCGGTCAGGAAGGGCGTCAAGCTGTGATGGCATCAGACTTTGCTATGGGGCAGTTTCGGTTTTTGAAAAGATTACTTCTGGTGCATGGGCACTGGAATTATCAGCGTGTTGGCTATTTGGTTCTGTACAACTTCTACCGCAATGCAGTTTTTGTAATGATGCTATTTTGGTATATCTTATCCACTGCATTTTCAACAACTTCTGCATTAACAGATTGGAGTAGTGTTTTCTATTCTGTCATTTATACTTCACTCCCTACAATTGTTGTTGGTATACTGGACAAAGACTTGAGCCACAGGACACTGTTACAATATCCGAAACTCTATGGTGCGGGCCATAGACACGAGGCATACAATTTACATCTCTTCTGGATCACAATGCTTGACACCCTATGGCAGAGTCTTGTTCTCTTCTATGTACCCCTCTTCACGTATAAGGATAGCTCAATAGACATATGGAGCATGGGCAGTTTATGGACAATAGCAGTTGTTGTTCTTGTCAACATACATTTGGCAATGGACATCCATCGTTGGGTATTCATCACTCATATTGCAGTATGGGGTTCAATAATCATCACATATGCCTGTATGATTGTATTGGATTCTATACCTGTCTTTCCTAATTACTGGACTATATACCATTTGGCAAAGTCTCCCACATATTGGATTGCCATTTTGCTTATAACAGTTGTCGCGTTGCTCCCTCGCTTTGTGTTCAAAGTTGTATATCATATTTTTTGGCCTTCAGATATCCAGATAGCTGCTGGGATTTTAAATAGACAACGTAAACATTTGAGCTCAAAGCAAGATGACAGTTCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1183

Amino Acids

133.77

Weight (kDa)

6.48

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhoLip_ATPase_N PF16209 50 - 115 2.1e-23 Phospholipid-translocating ATPase N-terminal
Cation_ATPase PF13246 550 - 714 2.4e-13 P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C PF16212 917 - 1157 3.6e-79 Phospholipid-translocating P-type ATPase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2282, 3407
AasI GACNNNNNNGTC 1 cut(s) 2056
Acc16I TGCGCA 1 cut(s) 1527
Acc36I ACCTGC 1 cut(s) 2072
AccB7I CCANNNNNTGG 1 cut(s) 3387
AccI GTMKAC 2 cut(s) 2520, 2998
AccII CGCG 2 cut(s) 1918, 3419
AciI CCGC 7 cut(s) 409, 836, 1602, 2705, 2727, 2863, 3056
AclWI GGATC 7 cut(s) 82, 95, 155, 566, 1995, 2689, 3104
AcoI YGGCCR 3 cut(s) 466, 972, 2319
AcsI RAATTY 7 cut(s) 25, 604, 761, 1352, 1381, 1458, 2721
AcuI CTGAAG 3 cut(s) 536, 827, 3453
AcyI GRCGYC 1 cut(s) 2740
AfaI GTAC 8 cut(s) 221, 679, 1588, 1966, 2041, 2644, 2852, 3144
AfiI CCNNNNNNNGG 4 cut(s) 1546, 2704, 3256, 3387
AflIII ACRYGT 1 cut(s) 1916
AjiI CACGTC 1 cut(s) 550
AjnI CCWGG 3 cut(s) 48, 1982, 2416
AjuI GAANNNNNNNTTGG 6 cut(s) 236, 268, 1742, 1774, 2176, 2208
AleI CACNNNNGTG 1 cut(s) 99
Alw21I GWGCWC 2 cut(s) 1479, 3524
Alw26I GTCTC 6 cut(s) 84, 351, 406, 2090, 2213, 3381
AlwI GGATC 7 cut(s) 82, 95, 155, 566, 1995, 2689, 3104
AlwNI CAGNNNCTG 1 cut(s) 1685
Ama87I CYCGRG 1 cut(s) 2368
AoxI GGCC 7 cut(s) 466, 972, 985, 1198, 2319, 3058, 3464
ApeKI GCWGC 7 cut(s) 124, 299, 878, 1523, 1550, 1679, 3485
ApoI RAATTY 7 cut(s) 25, 604, 761, 1352, 1381, 1458, 2721
ArsI GACNNNNNNTTYG 4 cut(s) 2068, 2100, 2212, 2244
Asp700I GAANNNNTTC 1 cut(s) 251
AspA2I CCTAGG 1 cut(s) 1501
AspLEI GCGC 3 cut(s) 1002, 1528, 1713
AspS9I GGNCC 4 cut(s) 985, 1198, 2058, 3058
AsuC2I CCSGG 1 cut(s) 443
AsuHPI GGTGA 4 cut(s) 626, 1757, 1861, 2681
AsuII TTCGAA 1 cut(s) 208
AvaI CYCGRG 1 cut(s) 2368
AvaII GGWCC 1 cut(s) 2058
AvrII CCTAGG 1 cut(s) 1501
BaeGI GKGCMC 2 cut(s) 2445, 2817
BalI TGGCCA 2 cut(s) 468, 974
BamHI GGATCC 1 cut(s) 87
BanII GRGCYC 3 cut(s) 693, 1479, 3524
BarI GAAGNNNNNNTAC 2 cut(s) 1758, 1790
BauI CACGAG 1 cut(s) 3068
BbsI GAAGAC 1 cut(s) 1227
Bbv12I GWGCWC 2 cut(s) 1479, 3524
BbvI GCAGC 7 cut(s) 111, 286, 890, 1510, 1537, 1691, 3472
BccI CCATC 8 cut(s) 386, 605, 1434, 2138, 2498, 2666, 2746, 3258
BceAI ACGGC 3 cut(s) 311, 2306, 2718
BcgI CGANNNNNNTGC 2 cut(s) 2025, 2059
BciT130I CCWGG 3 cut(s) 50, 1984, 2418
BciVI GTATCC 1 cut(s) 1092
BclI TGATCA 1 cut(s) 1906
BcnI CCSGG 1 cut(s) 443
BcoDI GTCTC 6 cut(s) 84, 351, 406, 2090, 2213, 3381
BfaI CTAG 5 cut(s) 60, 213, 290, 576, 1502
BfmI CTRYAG 5 cut(s) 1084, 1464, 1677, 2572, 2664
BfuAI ACCTGC 1 cut(s) 2072
BfuI GTATCC 1 cut(s) 1092
BglII AGATCT 3 cut(s) 1110, 1268, 2536
BisI GCNGC 7 cut(s) 125, 300, 879, 1524, 1551, 1680, 3486
BlnI CCTAGG 1 cut(s) 1501
BlsI GCNGC 7 cut(s) 126, 301, 880, 1525, 1552, 1681, 3487
Bme1390I CCNGG 4 cut(s) 50, 443, 1984, 2418
Bme18I GGWCC 1 cut(s) 2058
BmeT110I CYCGRG 1 cut(s) 2368
BmgBI CACGTC 1 cut(s) 550
BmgT120I GGNCC 4 cut(s) 985, 1198, 2058, 3058
BmiI GGNNCC 2 cut(s) 89, 1569
BmrFI CCNGG 4 cut(s) 50, 443, 1984, 2418
BmrI ACTGGG 2 cut(s) 204, 829
BmsI GCATC 3 cut(s) 2023, 2765, 2874
BmuI ACTGGG 2 cut(s) 204, 829
BpiI GAAGAC 1 cut(s) 1227
BpmI CTGGAG 3 cut(s) 2226, 2549, 2561
Bpu10I CCTNAGC 1 cut(s) 770
Bpu14I TTCGAA 1 cut(s) 208
BpuEI CTTGAG 4 cut(s) 1237, 2027, 2429, 3034
BpuMI CCSGG 1 cut(s) 443
Bsa29I ATCGAT 1 cut(s) 941
BsaAI YACGTR 2 cut(s) 1964, 3156
BsaBI GATNNNNATC 2 cut(s) 135, 1160
BsaHI GRCGYC 1 cut(s) 2740
BsaI GGTCTC 1 cut(s) 2090
BsaJI CCNNGG 7 cut(s) 48, 49, 140, 858, 1023, 1501, 1983
BsaWI WCCGGW 1 cut(s) 1035
Bsc4I CCNNNNNNNGG 4 cut(s) 1546, 2704, 3256, 3387
Bse3DI GCAATG 4 cut(s) 870, 2428, 2872, 3246
Bse8I GATNNNNATC 2 cut(s) 135, 1160
BseBI CCWGG 3 cut(s) 50, 1984, 2418
BseCI ATCGAT 1 cut(s) 941
BseDI CCNNGG 7 cut(s) 48, 49, 140, 858, 1023, 1501, 1983
BseJI GATNNNNATC 2 cut(s) 135, 1160
BseLI CCNNNNNNNGG 4 cut(s) 1546, 2704, 3256, 3387
BseMI GCAATG 4 cut(s) 870, 2428, 2872, 3246
BseMII CTCAG 5 cut(s) 95, 761, 1928, 1940, 2286
BseSI GKGCMC 2 cut(s) 2445, 2817
BseXI GCAGC 7 cut(s) 111, 286, 890, 1510, 1537, 1691, 3472
BseYI CCCAGC 1 cut(s) 3488
BsgI GTGCAG 5 cut(s) 110, 125, 897, 984, 1285
Bsh1236I CGCG 2 cut(s) 1918, 3419
Bsh1285I CGRYCG 1 cut(s) 479
BshFI GGCC 7 cut(s) 468, 974, 987, 1200, 2321, 3060, 3466
BshVI ATCGAT 1 cut(s) 941
BsiEI CGRYCG 1 cut(s) 479
BsiHKAI GWGCWC 2 cut(s) 1479, 3524
BsiHKCI CYCGRG 1 cut(s) 2368
BsiSI CCGG 3 cut(s) 442, 1036, 2173
BslFI GGGAC 5 cut(s) 577, 1342, 1402, 1785, 2152
BslI CCNNNNNNNGG 4 cut(s) 1546, 2704, 3256, 3387
BsmAI GTCTC 6 cut(s) 84, 351, 406, 2090, 2213, 3381
BsmFI GGGAC 5 cut(s) 577, 1342, 1402, 1785, 2152
BsmI GAATGC 3 cut(s) 2099, 2309, 2485
BsnI GGCC 7 cut(s) 468, 974, 987, 1200, 2321, 3060, 3466
Bso31I GGTCTC 1 cut(s) 2090
BsoBI CYCGRG 1 cut(s) 2368
Bsp119I TTCGAA 1 cut(s) 208
Bsp1286I GDGCHC 5 cut(s) 693, 1479, 2445, 2817, 3524
Bsp1407I TGTACA 2 cut(s) 677, 2850
BspACI CCGC 7 cut(s) 409, 836, 1602, 2705, 2727, 2863, 3056
BspANI GGCC 7 cut(s) 468, 974, 987, 1200, 2321, 3060, 3466
BspCNI CTCAG 5 cut(s) 94, 762, 1929, 1939, 2287
BspDI ATCGAT 1 cut(s) 941
BspFNI CGCG 2 cut(s) 1918, 3419
BspHI TCATGA 2 cut(s) 1212, 1777
BspLI GGNNCC 2 cut(s) 89, 1569
BspMAI CTGCAG 1 cut(s) 1681
BspMI ACCTGC 1 cut(s) 2072
BspPI GGATC 7 cut(s) 82, 95, 155, 566, 1995, 2689, 3104
BspQI GCTCTTC 1 cut(s) 1354
BspT104I TTCGAA 1 cut(s) 208
BspTNI GGTCTC 1 cut(s) 2090
BsrDI GCAATG 4 cut(s) 870, 2428, 2872, 3246
BsrGI TGTACA 2 cut(s) 677, 2850
BssECI CCNNGG 7 cut(s) 48, 49, 140, 858, 1023, 1501, 1983
BssNAI GTATAC 2 cut(s) 2521, 2999
BssNI GRCGYC 1 cut(s) 2740
BssSI CACGAG 1 cut(s) 3068
BssT1I CCWWGG 4 cut(s) 140, 858, 1023, 1501
Bst1107I GTATAC 2 cut(s) 2521, 2999
Bst2BI CACGAG 1 cut(s) 3068
Bst2UI CCWGG 3 cut(s) 50, 1984, 2418
Bst4CI ACNGT 8 cut(s) 343, 664, 1241, 1564, 2513, 3030, 3412, 3539
Bst6I CTCTTC 5 cut(s) 161, 1354, 3095, 3140, 3155
BstACI GRCGYC 1 cut(s) 2740
BstAPI GCANNNNNTGC 3 cut(s) 299, 1412, 1685
BstAUI TGTACA 2 cut(s) 677, 2850
BstBAI YACGTR 2 cut(s) 1964, 3156
BstBI TTCGAA 1 cut(s) 208
BstC8I GCNNGC 7 cut(s) 803, 1366, 1555, 1665, 1793, 2178, 3058
BstDEI CTNAG 6 cut(s) 81, 770, 1754, 1926, 1937, 2295
BstFNI CGCG 2 cut(s) 1918, 3419
BstHHI GCGC 3 cut(s) 1002, 1528, 1713
BstMAI GTCTC 6 cut(s) 84, 351, 406, 2090, 2213, 3381
BstMCI CGRYCG 1 cut(s) 479
BstMWI GCNNNNNNNGC 7 cut(s) 299, 842, 971, 1412, 1685, 2489, 2753
BstNI CCWGG 3 cut(s) 50, 1984, 2418
BstNSI RCATGY 1 cut(s) 2026
BstSCI CCNGG 4 cut(s) 48, 441, 1982, 2416
BstSFI CTRYAG 5 cut(s) 1084, 1464, 1677, 2572, 2664
BstSLI GKGCMC 2 cut(s) 2445, 2817
BstSNI TACGTA 1 cut(s) 1964
BstUI CGCG 2 cut(s) 1918, 3419
BstV1I GCAGC 7 cut(s) 111, 286, 890, 1510, 1537, 1691, 3472
BstV2I GAAGAC 1 cut(s) 1227
BstX2I RGATCY 5 cut(s) 87, 1110, 1268, 1987, 2536
BstXI CCANNNNNNTGG 2 cut(s) 982, 2453
BstYI RGATCY 5 cut(s) 87, 1110, 1268, 1987, 2536
BstZ17I GTATAC 2 cut(s) 2521, 2999
Bsu15I ATCGAT 1 cut(s) 941
BsuI GTATCC 1 cut(s) 1092
BsuRI GGCC 7 cut(s) 468, 974, 987, 1200, 2321, 3060, 3466
BsuTUI ATCGAT 1 cut(s) 941
BtrI CACGTC 1 cut(s) 550
BtsI GCAGTG 3 cut(s) 732, 2018, 2907
BveI ACCTGC 1 cut(s) 2072
Cac8I GCNNGC 7 cut(s) 803, 1366, 1555, 1665, 1793, 2178, 3058
CaiI CAGNNNCTG 1 cut(s) 1685
CciI TCATGA 2 cut(s) 1212, 1777
CfoI GCGC 3 cut(s) 1002, 1528, 1713
Cfr13I GGNCC 4 cut(s) 985, 1198, 2058, 3058
ClaI ATCGAT 1 cut(s) 941
CseI GACGC 2 cut(s) 1924, 2729
Csp6I GTAC 8 cut(s) 220, 678, 1587, 1965, 2040, 2643, 2851, 3143
CviQI GTAC 8 cut(s) 220, 678, 1587, 1965, 2040, 2643, 2851, 3143
DdeI CTNAG 6 cut(s) 81, 770, 1754, 1926, 1937, 2295
DraI TTTAAA 1 cut(s) 3498
DrdI GACNNNNNNGTC 1 cut(s) 2056
DseDI GACNNNNNNGTC 1 cut(s) 2056
EaeI YGGCCR 3 cut(s) 466, 972, 2319
Eam1104I CTCTTC 5 cut(s) 161, 1354, 3095, 3140, 3155
EarI CTCTTC 5 cut(s) 161, 1354, 3095, 3140, 3155
EciI GGCGGA 2 cut(s) 424, 2720
Ecl136II GAGCTC 2 cut(s) 1477, 3522
Eco105I TACGTA 1 cut(s) 1964
Eco130I CCWWGG 4 cut(s) 140, 858, 1023, 1501
Eco24I GRGCYC 3 cut(s) 693, 1479, 3524
Eco31I GGTCTC 1 cut(s) 2090
Eco32I GATATC 1 cut(s) 3475
Eco47I GGWCC 1 cut(s) 2058
Eco53kI GAGCTC 2 cut(s) 1477, 3522
Eco57I CTGAAG 3 cut(s) 536, 827, 3453
Eco88I CYCGRG 1 cut(s) 2368
EcoICRI GAGCTC 2 cut(s) 1477, 3522
EcoRI GAATTC 1 cut(s) 1352
EcoRII CCWGG 3 cut(s) 48, 1982, 2416
EcoRV GATATC 1 cut(s) 3475
EcoT14I CCWWGG 4 cut(s) 140, 858, 1023, 1501
EcoT38I GRGCYC 3 cut(s) 693, 1479, 3524
ErhI CCWWGG 4 cut(s) 140, 858, 1023, 1501
FaqI GGGAC 5 cut(s) 577, 1342, 1402, 1785, 2152
FauI CCCGC 1 cut(s) 3049
FauNDI CATATG 5 cut(s) 1054, 1230, 1690, 3179, 3304
FbaI TGATCA 1 cut(s) 1906
FblI GTMKAC 2 cut(s) 2520, 2998
Fnu4HI GCNGC 7 cut(s) 125, 300, 879, 1524, 1551, 1680, 3486
FriOI GRGCYC 3 cut(s) 693, 1479, 3524
Fsp4HI GCNGC 7 cut(s) 125, 300, 879, 1524, 1551, 1680, 3486
FspBI CTAG 5 cut(s) 60, 213, 290, 576, 1502
FspI TGCGCA 1 cut(s) 1527
GlaI GCGC 3 cut(s) 1001, 1527, 1712
GluI GCNGC 7 cut(s) 125, 300, 879, 1524, 1551, 1680, 3486
GsaI CCCAGC 1 cut(s) 3492
GsuI CTGGAG 3 cut(s) 2226, 2549, 2561
HaeIII GGCC 7 cut(s) 468, 974, 987, 1200, 2321, 3060, 3466
HapII CCGG 3 cut(s) 442, 1036, 2173
HgaI GACGC 2 cut(s) 1924, 2729
HhaI GCGC 3 cut(s) 1002, 1528, 1713
Hin1I GRCGYC 1 cut(s) 2740
Hin6I GCGC 3 cut(s) 1000, 1526, 1711
HinP1I GCGC 3 cut(s) 1000, 1526, 1711
HincII GTYRAC 2 cut(s) 2049, 3226
HindII GTYRAC 2 cut(s) 2049, 3226
HindIII AAGCTT 1 cut(s) 2105
HpaII CCGG 3 cut(s) 442, 1036, 2173
HphI GGTGA 4 cut(s) 626, 1757, 1861, 2681
HpyAV CCTTC 9 cut(s) 244, 368, 665, 897, 1949, 2455, 2462, 2729, 3477
HpyCH4III ACNGT 8 cut(s) 343, 664, 1241, 1564, 2513, 3030, 3412, 3539
HpyCH4IV ACGT 6 cut(s) 549, 1544, 1963, 2071, 3155, 3508
HpyF10VI GCNNNNNNNGC 7 cut(s) 299, 842, 971, 1412, 1685, 2489, 2753
HpyF3I CTNAG 6 cut(s) 81, 770, 1754, 1926, 1937, 2295
HpySE526I ACGT 6 cut(s) 549, 1544, 1963, 2071, 3155, 3508
Hsp92I GRCGYC 1 cut(s) 2740
HspAI GCGC 3 cut(s) 1000, 1526, 1711
Ksp22I TGATCA 1 cut(s) 1906
LguI GCTCTTC 1 cut(s) 1354
LmnI GCTCC 3 cut(s) 2675, 3183, 3429
Lsp1109I GCAGC 7 cut(s) 111, 286, 890, 1510, 1537, 1691, 3472
LweI GCATC 3 cut(s) 2023, 2765, 2874
MaeI CTAG 5 cut(s) 60, 213, 290, 576, 1502
MaeII ACGT 6 cut(s) 549, 1544, 1963, 2071, 3155, 3508
MaeIII GTNAC 5 cut(s) 92, 376, 545, 1912, 3030
MfeI CAATTG 3 cut(s) 294, 2124, 2985
MflI RGATCY 5 cut(s) 87, 1110, 1268, 1987, 2536
MhlI GDGCHC 5 cut(s) 693, 1479, 2445, 2817, 3524
MlsI TGGCCA 2 cut(s) 468, 974
MluI ACGCGT 1 cut(s) 1916
MluNI TGGCCA 2 cut(s) 468, 974
MlyI GAGTC 5 cut(s) 626, 1886, 2174, 2552, 3133
MmeI TCCRAC 3 cut(s) 1740, 2692, 2698
Mox20I TGGCCA 2 cut(s) 468, 974
MroXI GAANNNNTTC 1 cut(s) 251
MscI TGGCCA 2 cut(s) 468, 974
MseI TTAA 7 cut(s) 156, 750, 1922, 2102, 2634, 2933, 3497
MslI CAYNNNNRTG 3 cut(s) 99, 980, 1938
Msp20I TGGCCA 2 cut(s) 468, 974
MspA1I CMGCKG 1 cut(s) 805
MspI CCGG 3 cut(s) 442, 1036, 2173
MspR9I CCNGG 4 cut(s) 50, 443, 1984, 2418
MunI CAATTG 3 cut(s) 294, 2124, 2985
Mva1269I GAATGC 3 cut(s) 2099, 2309, 2485
MvaI CCWGG 3 cut(s) 50, 1984, 2418
MvnI CGCG 2 cut(s) 1918, 3419
MwoI GCNNNNNNNGC 7 cut(s) 299, 842, 971, 1412, 1685, 2489, 2753
NciI CCSGG 1 cut(s) 443
NdeI CATATG 5 cut(s) 1054, 1230, 1690, 3179, 3304
NlaIV GGNNCC 2 cut(s) 89, 1569
NmeAIII GCCGAG 1 cut(s) 622
NmuCI GTSAC 4 cut(s) 92, 376, 545, 1912
NsbI TGCGCA 1 cut(s) 1527
NspI RCATGY 1 cut(s) 2026
NspV TTCGAA 1 cut(s) 208
OliI CACNNNNGTG 1 cut(s) 99
PagI TCATGA 2 cut(s) 1212, 1777
PasI CCCWGGG 1 cut(s) 49
PciSI GCTCTTC 1 cut(s) 1354
PctI GAATGC 3 cut(s) 2099, 2309, 2485
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 9 cut(s) 205, 247, 1044, 1257, 1419, 1649, 1930, 2184, 3326
PflMI CCANNNNNTGG 1 cut(s) 3387
PkrI GCNGC 7 cut(s) 126, 301, 880, 1525, 1552, 1681, 3487
PleI GAGTC 5 cut(s) 625, 1886, 2173, 2551, 3132
PpsI GAGTC 5 cut(s) 625, 1886, 2173, 2551, 3132
Ppu21I YACGTR 2 cut(s) 1964, 3156
PsiI TTATAA 2 cut(s) 2282, 3407
Psp124BI GAGCTC 2 cut(s) 1479, 3524
Psp6I CCWGG 3 cut(s) 48, 1982, 2416
PspFI CCCAGC 1 cut(s) 3488
PspGI CCWGG 3 cut(s) 48, 1982, 2416
PspN4I GGNNCC 2 cut(s) 89, 1569
PspPI GGNCC 4 cut(s) 985, 1198, 2058, 3058
PstI CTGCAG 1 cut(s) 1681
PstNI CAGNNNCTG 1 cut(s) 1685
PsuI RGATCY 5 cut(s) 87, 1110, 1268, 1987, 2536
PvuII CAGCTG 1 cut(s) 805
RsaI GTAC 8 cut(s) 221, 679, 1588, 1966, 2041, 2644, 2852, 3144
RsaNI GTAC 8 cut(s) 220, 678, 1587, 1965, 2040, 2643, 2851, 3143
RseI CAYNNNNRTG 3 cut(s) 99, 980, 1938
SacI GAGCTC 2 cut(s) 1479, 3524
SapI GCTCTTC 1 cut(s) 1354
SaqAI TTAA 7 cut(s) 156, 750, 1922, 2102, 2634, 2933, 3497
SatI GCNGC 7 cut(s) 125, 300, 879, 1524, 1551, 1680, 3486
Sau96I GGNCC 4 cut(s) 985, 1198, 2058, 3058
SchI GAGTC 5 cut(s) 626, 1886, 2174, 2552, 3133
ScrFI CCNGG 4 cut(s) 50, 443, 1984, 2418
SduI GDGCHC 5 cut(s) 693, 1479, 2445, 2817, 3524
SfaNI GCATC 3 cut(s) 2023, 2765, 2874
SfcI CTRYAG 5 cut(s) 1084, 1464, 1677, 2572, 2664
SfuI TTCGAA 1 cut(s) 208
SinI GGWCC 1 cut(s) 2058
SmiMI CAYNNNNRTG 3 cut(s) 99, 980, 1938
SmlI CTYRAG 4 cut(s) 1252, 2006, 2408, 3013
SmoI CTYRAG 4 cut(s) 1252, 2006, 2408, 3013
SnaBI TACGTA 1 cut(s) 1964
SsiI CCGC 7 cut(s) 409, 836, 1602, 2705, 2727, 2863, 3056
SspI AATATT 1 cut(s) 1946
SspMI CTAG 5 cut(s) 60, 213, 290, 576, 1502
SstI GAGCTC 2 cut(s) 1479, 3524
StyD4I CCNGG 4 cut(s) 48, 441, 1982, 2416
StyI CCWWGG 4 cut(s) 140, 858, 1023, 1501
TaaI ACNGT 8 cut(s) 343, 664, 1241, 1564, 2513, 3030, 3412, 3539
TaiI ACGT 6 cut(s) 552, 1547, 1966, 2074, 3158, 3511
TaqI TCGA 8 cut(s) 111, 208, 475, 620, 941, 1192, 2063, 2231
TaqII GACCGA 2 cut(s) 493, 2046
TatI WGTACW 3 cut(s) 219, 677, 2850
TfiI GAWTC 9 cut(s) 205, 247, 1044, 1257, 1419, 1649, 1930, 2184, 3326
Tru1I TTAA 7 cut(s) 156, 750, 1922, 2102, 2634, 2933, 3497
Tru9I TTAA 7 cut(s) 156, 750, 1922, 2102, 2634, 2933, 3497
TseFI GTSAC 4 cut(s) 92, 376, 545, 1912
TseI GCWGC 7 cut(s) 124, 299, 878, 1523, 1550, 1679, 3485
Tsp45I GTSAC 4 cut(s) 92, 376, 545, 1912
TspGWI ACGGA 2 cut(s) 80, 1798
Van91I CCANNNNNTGG 1 cut(s) 3387
VpaK11BI GGWCC 1 cut(s) 2058
XapI RAATTY 7 cut(s) 25, 604, 761, 1352, 1381, 1458, 2721
XceI RCATGY 1 cut(s) 2026
XcmI CCANNNNNNNNNTGG 1 cut(s) 3485
XmaJI CCTAGG 1 cut(s) 1501
XmiI GTMKAC 2 cut(s) 2520, 2998
XmnI GAANNNNTTC 1 cut(s) 251
XspI CTAG 5 cut(s) 60, 213, 290, 576, 1502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.