RLG00000025020

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0009696
Physical Location & Seq
Reverse (-)
41379098 .. 41383974
4877 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025020

Sequence Viewer

Length: 3879 bp
ATGAGCGGTGAGCATCCGTTGCTGTCATCACCGGATTCTCCATCAAAGCCACTTGTGCCGCCCTCTTCTCTAGCTGAGAGACTTGAGCAGCTCTGCCTTGATGAATCCAGTTCTTCTTCCATTCTTGACGGCATTGATGATGGCCAAGGTGAATCGTTGTTAGCGAGGGAATACACTGCTGCTCCTGACTTCGGTGTGAAACCAGGAAAGCAGTTTTTTCCGGTAGAGCGTGAGTGCCGGCCGCAGTTTCCGTTGGAATACCCCACGCGGCATAAAAAACGCCAGGTATCGTGGGGTACAATGGAACTGCATAGTATCAATAGTAATTCAGCGTCCCATGATGTCCCCCAGGCTCCATCTAGGGTTCTGGAAAAGGCGAACAAGTCTCAGGGGAGTCACCACAAAGGCATTCAATTTGAAGATAACCTCCTACATGAAGACAATTCAAGGTTGATCTATATTGATGATCCGAAGAAAACAAATGACAAGTATGAGTTCACTGGCAATGAGATTCGAACTAGCAAGTACACCTTAATCACCTTCTTGCCCAAGAATGTTTTCATTCAGTTTCACCGGGTTGCTTATTTGTATTTTCTAGCAATTGCTGTTCTCAATCAGCTTCCGCCTCTTGCTGTGTTTGGAAGAACAGTGTCTCTTTTCCCCCTCCTATTTGTGCTGTGTGTCACTGCTATAAAAGATGGCTATGAGGATTGGCGAAGACATAGATCAGACAGGAATGAAAACAATCGGGAGTCTCTGATCCTTCAATCTGGGCAATTTCAACCAAAGAAATGGAAAAATATTCAAGTGGGCGAGGTTCTGAAGATTTGTGCTGATGATACAATTCCTTGTGATATAGTTTTGTTAGGAACGAGTGACCCTAGTGGAATTGCCTACATTCAGACAATGAATCTGGATGGCGAGTCAAACCTCAAAACAAGGTATGCACGCCAGGAAACATCTTCAGCTGTAGGTTCAGCAGTAGCCGATGGGTGTGCAATTATGGGGATCATCAGATGCGAACAACCAAATCGAAATATCTATGAGTTTACAGCCAACATGGAGTTTAAAGGACATAGATTTCCCCTAACACAGTCAAACATAGTTTTGCGTGGTTGCCAGCTGAAGAACACGGAGTGGATAATTGGTGTTGTGGTATATGCTGGACAGGAAACAAAGGCAATGTTGAATAGTGCAGCTTCTCCTTCCAAGAGAAGCAAACTTGAAGGCTACATGAACAGAGAAACCTTGTGGTTGTCAATTTTCCTTTTTGTCATGTGTTCAGTTGTGGCTGCTGGCATGGGCACGTGGCTAATACGCCATAAATATCAGGTTGATACGTTGCCTTATTACCGGAAAAGATTCTTCACTAACTGGGGGAAGCTTAATGGGAAAACCTATAAATATTATGGGATACCTATGGAGATTTTTTTCTCCTTTTTGAGTTCTGTCATAGTGTTTCAGATAATGATACCAATTGCTCTGTATATTAGTATTGAGTTGGTTCGTTTGGGCCAGTCATATTTCATGATTGAAGACAGGCATATGTTCGACTGTAGCTCTGGCTCAAGGTTCCAGTGCAGATCCTTGAATATCAATGAGGATCTGGGTCAAATACGATATATATTTTCAGACAAAACAGGGACACTTACTGAAAATAAGATGGCATTCCGCAGAGCAAGCATATATGGGCGGGATTATGGGAGCCGTGTGCTTGTGGCTGATCAATTGGAGGAAGAAAATAATACAGGAGAAGGTTTAGCTAGAAAGAGATGGAAGCTTAAATCTGAAGTTGCTGTCGATTATGAGCTCATGGAGTTGTTGCACAAAGACTTGAGTGAAGATGAGAGGATTGCTGCACATGAGTTTTTCCTTACGTTGGCAGCTTGCAATACTGTGGTTCCTATAGTCAGTACTAGTACATCTTCCAGTTGTGCTAAGGGTGATGTTGATTCTATTGATTATCAGGGGGAATCCCCGGACGAGCAAGCACTAGTTACTGCAGCCTCTGGTTATAGATATACTCTCTTTGAGCGCACATCGGGACATATTGCAATTGATGTCAATGGTGAGAAACTAAGGTTGGATGTATTGGGTCTGCATGAATTTGACAGTGTGCGAAAACGAATGTCAGTTGTTATCAGATTTCCAAACAATACTATAAAGGTATTGGTGAAAGGTGCTGATACCTCTATGCTCAGCATATTAGCGAATGACTCTGAAAGAGATGATGAACTGAGACATTCAACTCAGAGCCATTTGAGTGAATATTCCTCACATGGTTTGCGTACTCTTGTGGTTGCTGCAAGAGATCTTACAAATGAAGAACTTGAACAGTGGCAAAGCATGTATGAAGATGCAAGTACCTCATTGACTGATCGATCCTTAAAACTACGTCAAACAGCAGCTCTTATTGAATCCAACTTAAAGCTGCTTGGGGCAACTGCAATTGAGGATAGGCTACAAGATGGTGTGCCAGAAGCTATTGAGTCCCTCAGGCAAGCAGGAATTAAGGTCTGGGTTTTGACTGGAGATAAGCAAGAGACAGCTATTTCAATTGGTGTATCATGCAAACTGTTGACAGCAGATATGCAACAAATTATCATAAATGGAACTTCTGAGGCTGAATGCAGAAATCTTTTGGTTGATGCTATGGAAAAATATGGTGTGCAGTCATCAAATAAAAGAAATCAGAGTTCAAAACGCGAAAGCAATGCTGCCAGTGATTATCTTGAGTTACCTGATGATGCTAAGACATTTAATGTGCCAAAATGGCATGAAGCAAAGGAAGAAGGAAAGATAAGTGCACCATTAGCACTCATAATTGATGGGAACAGTTTGGTATACATTTTGGAGAAAGATCTGCAGTCGGAGCTTTTCGACCTTGCCACTTCCTGTAGTGTGGTTGTATGCTGTCGTGTTGCACCATTGCAGAAAGCTGGAATCGTTGATCTAATTAAGACCCGCACTGATGATATGACACTGGCTATAGGTGATGGGGCAAATGATGTTTCTATGATCCAAATGGCGGATGTTGGTGTTGGAATTTGTGGTCAGGAGGGTCGTCAAGCTGTGATGGCATCAGACTTTGCCATGGGACAGTTTCGGTTTTTGAAAAGATTACTTCTGGTGCATGGACACTGGAATTATCAGCGTATTGGTTATTTGATTCTTTACAACTTCTACCGCAACGCAGTTTTTGTGTTGATGCTATTTTGGTATATATTGTCCACGGCATTTTCAACAACTTCGGCGTTGACAGATTGGAGTAGTGTTTTCTATTCTCTTATATATACGTCTATCCCTACAATTATTGTTGGTGTACTGGACAAAGATCTGAGCCACAAGACCCTGTTACAATACCCAAAATTGTATGGTGCTGGCCATAGACAGGAAGCATATAATCCAAATCTTTTCTGGATAACGATGCTTGACACCTTATGGCAGAGTCTTGTTCTCTTCTATGTACCCCAGTTCACCTATAAGGAGAGCACAATAGACATATGGAGCATGGGCAGTTTGTGGACCATAGCAGTAGTTATCCTTGTTAATGTACATTTGGCAATGGACATTCATCGGTGGGTATTTATCACTCATATTGCAGTATGGGGATCAATAATCATCACATATGCCTGTGTGGTGATATTGGATTCCATACCTGTTTATCCCAATTACTGGACTTTTTACCATTTGGCATGTTCTCCTACCTATTGGATCACCATTTTGCTTATAATAGTTGTAGCCTTGCTCCCTCGTTTTGTGTACAAAGTTGTACATCATATTTTTTGGCCCTCAGATATCCAGATAGCTAGAGAAGCTGAGATATTAAGTAGGAAACATAAGCATTTGGGCTCAGACAAAGATGAAGATTCCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1293

Amino Acids

146.03

Weight (kDa)

6.02

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhoLip_ATPase_N PF16209 152 - 217 4.1e-22 Phospholipid-translocating ATPase N-terminal
Cation_ATPase PF13246 658 - 822 4.2e-14 P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C PF16212 1025 - 1265 1.4e-79 Phospholipid-translocating P-type ATPase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3731
AccB7I CCANNNNNTGG 1 cut(s) 3675
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 2844
AccII CGCG 2 cut(s) 268, 2706
AclWI GGATC 9 cut(s) 461, 754, 1016, 1578, 1611, 2376, 3013, 3619, 3722
AcoI YGGCCR 3 cut(s) 142, 239, 3382
AcsI RAATTY 2 cut(s) 2105, 3045
AcuI CTGAAG 4 cut(s) 842, 948, 1145, 1809
AcvI CACGTG 1 cut(s) 1308
AdeI CACNNNGTG 1 cut(s) 1137
AfiI CCNNNNNNNGG 5 cut(s) 191, 1879, 3028, 3391, 3675
AhlI ACTAGT 2 cut(s) 1916, 1993
AjnI CCWGG 4 cut(s) 202, 282, 348, 951
AjuI GAANNNNNNNTTGG 4 cut(s) 542, 574, 2066, 2098
Alw21I GWGCWC 3 cut(s) 1812, 2809, 3494
Alw26I GTCTC 6 cut(s) 73, 390, 657, 759, 2233, 2537
Alw44I GTGCAC 1 cut(s) 2805
AlwI GGATC 9 cut(s) 461, 754, 1016, 1578, 1611, 2376, 3013, 3619, 3722
AlwNI CAGNNNCTG 1 cut(s) 2009
AoxI GGCC 5 cut(s) 142, 239, 1513, 3382, 3788
ApaLI GTGCAC 1 cut(s) 2805
ApoI RAATTY 2 cut(s) 2105, 3045
Asp700I GAANNNNTTC 2 cut(s) 557, 1361
AspLEI GCGC 1 cut(s) 2037
AspS9I GGNCC 3 cut(s) 1513, 3525, 3789
AsuC2I CCSGG 2 cut(s) 575, 1979
AsuII TTCGAA 1 cut(s) 514
AvaII GGWCC 1 cut(s) 3525
AxyI CCTNAGG 1 cut(s) 2495
BaeGI GKGCMC 2 cut(s) 1307, 2809
BalI TGGCCA 2 cut(s) 144, 3384
BanII GRGCYC 2 cut(s) 1812, 3854
BbrPI CACGTG 1 cut(s) 1308
BbsI GAAGAC 3 cut(s) 444, 724, 1542
Bbv12I GWGCWC 3 cut(s) 1812, 2809, 3494
BceAI ACGGC 3 cut(s) 145, 1692, 3249
BcgI CGANNNNNNTGC 4 cut(s) 977, 1011, 2696, 2730
BciT130I CCWGG 4 cut(s) 204, 284, 350, 953
BciVI GTATCC 1 cut(s) 1407
BclI TGATCA 1 cut(s) 1723
BcnI CCSGG 2 cut(s) 575, 1979
BcoDI GTCTC 6 cut(s) 73, 390, 657, 759, 2233, 2537
BcuI ACTAGT 2 cut(s) 1916, 1993
BfaI CTAG 9 cut(s) 71, 360, 519, 596, 882, 1764, 1917, 1994, 3810
BfmI CTRYAG 7 cut(s) 969, 1555, 1905, 2001, 2864, 2896, 2988
BfuI GTATCC 1 cut(s) 1407
BglI GCCNNNNNGGC 1 cut(s) 2773
BglII AGATCT 3 cut(s) 2311, 2860, 3334
BlpI GCTNAGC 1 cut(s) 2198
BmcAI AGTACT 1 cut(s) 1915
Bme1390I CCNGG 6 cut(s) 204, 284, 350, 575, 953, 1979
Bme18I GGWCC 1 cut(s) 3525
BmgT120I GGNCC 3 cut(s) 1513, 3525, 3789
BmiI GGNNCC 4 cut(s) 354, 1574, 1706, 1902
BmrFI CCNGG 6 cut(s) 204, 284, 350, 575, 953, 1979
BmrI ACTGGG 2 cut(s) 1384, 3466
BmsI GCATC 8 cut(s) 22, 1007, 2347, 2638, 2737, 3089, 3198, 3417
BmuI ACTGGG 2 cut(s) 1384, 3466
BpiI GAAGAC 3 cut(s) 444, 724, 1542
BpmI CTGGAG 1 cut(s) 2550
Bpu10I CCTNAGC 1 cut(s) 1938
Bpu1102I GCTNAGC 1 cut(s) 2198
Bpu14I TTCGAA 1 cut(s) 514
BpuEI CTTGAG 4 cut(s) 104, 1552, 1855, 2753
BpuMI CCSGG 2 cut(s) 575, 1979
Bsa29I ATCGAT 1 cut(s) 2380
BsaAI YACGTR 1 cut(s) 1308
BsaJI CCNNGG 5 cut(s) 145, 348, 1977, 3093, 3231
BsaWI WCCGGW 4 cut(s) 31, 220, 1353, 3872
BsaXI ACNNNNNCTCC 2 cut(s) 166, 196
Bsc4I CCNNNNNNNGG 5 cut(s) 191, 1879, 3028, 3391, 3675
Bse118I RCCGGY 1 cut(s) 237
Bse21I CCTNAGG 1 cut(s) 2495
Bse3DI GCAATG 5 cut(s) 511, 1188, 2719, 2927, 3570
BseBI CCWGG 4 cut(s) 204, 284, 350, 953
BseCI ATCGAT 1 cut(s) 2380
BseDI CCNNGG 5 cut(s) 145, 348, 1977, 3093, 3231
BseGI GGATG 4 cut(s) 13, 922, 2092, 3037
BseLI CCNNNNNNNGG 5 cut(s) 191, 1879, 3028, 3391, 3675
BseMI GCAATG 5 cut(s) 511, 1188, 2719, 2927, 3570
BseSI GKGCMC 2 cut(s) 1307, 2809
BseX3I CGGCCG 1 cut(s) 239
BsgI GTGCAG 4 cut(s) 1215, 1600, 1842, 2690
Bsh1236I CGCG 2 cut(s) 268, 2706
Bsh1285I CGRYCG 1 cut(s) 242
BshFI GGCC 5 cut(s) 144, 241, 1515, 3384, 3790
BshVI ATCGAT 1 cut(s) 2380
BsiEI CGRYCG 1 cut(s) 242
BsiHKAI GWGCWC 3 cut(s) 1812, 2809, 3494
BsiSI CCGG 7 cut(s) 32, 221, 238, 574, 1354, 1979, 3873
BslFI GGGAC 6 cut(s) 319, 329, 1657, 2058, 2476, 3111
BslI CCNNNNNNNGG 5 cut(s) 191, 1879, 3028, 3391, 3675
BsmAI GTCTC 6 cut(s) 73, 390, 657, 759, 2233, 2537
BsmFI GGGAC 6 cut(s) 319, 329, 1657, 2058, 2476, 3111
BsmI GAATGC 3 cut(s) 408, 1667, 2633
BsnI GGCC 5 cut(s) 144, 241, 1515, 3384, 3790
Bsp119I TTCGAA 1 cut(s) 514
Bsp1286I GDGCHC 5 cut(s) 1307, 1812, 2809, 3494, 3854
Bsp1407I TGTACA 3 cut(s) 3553, 3762, 3772
Bsp1720I GCTNAGC 1 cut(s) 2198
Bsp19I CCATGG 1 cut(s) 3093
BspANI GGCC 5 cut(s) 144, 241, 1515, 3384, 3790
BspDI ATCGAT 1 cut(s) 2380
BspFNI CGCG 2 cut(s) 268, 2706
BspHI TCATGA 1 cut(s) 1527
BspLI GGNNCC 4 cut(s) 354, 1574, 1706, 1902
BspMAI CTGCAG 2 cut(s) 2005, 2868
BspPI GGATC 9 cut(s) 461, 754, 1016, 1578, 1611, 2376, 3013, 3619, 3722
BspT104I TTCGAA 1 cut(s) 514
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 5 cut(s) 511, 1188, 2719, 2927, 3570
BsrFI RCCGGY 1 cut(s) 237
BsrGI TGTACA 3 cut(s) 3553, 3762, 3772
BssAI RCCGGY 1 cut(s) 237
BssECI CCNNGG 5 cut(s) 145, 348, 1977, 3093, 3231
BssNAI GTATAC 1 cut(s) 2845
BssT1I CCWWGG 2 cut(s) 145, 3093
Bst1107I GTATAC 1 cut(s) 2845
Bst2UI CCWGG 4 cut(s) 204, 284, 350, 953
Bst4CI ACNGT 9 cut(s) 649, 1095, 1556, 1897, 2114, 2337, 2577, 2837, 3102
Bst6I CTCTTC 2 cut(s) 70, 3464
BstAPI GCANNNNNTGC 1 cut(s) 19
BstAUI TGTACA 3 cut(s) 3553, 3762, 3772
BstBAI YACGTR 1 cut(s) 1308
BstBI TTCGAA 1 cut(s) 514
BstC8I GCNNGC 9 cut(s) 239, 949, 1121, 1297, 1681, 1888, 1989, 2502, 3382
BstDSI CCRYGG 2 cut(s) 3093, 3231
BstF5I GGATG 4 cut(s) 13, 922, 2092, 3037
BstFNI CGCG 2 cut(s) 268, 2706
BstHHI GCGC 1 cut(s) 2037
BstMAI GTCTC 6 cut(s) 73, 390, 657, 759, 2233, 2537
BstMCI CGRYCG 1 cut(s) 242
BstMWI GCNNNNNNNGC 8 cut(s) 19, 55, 1680, 2773, 2813, 2872, 3077, 3815
BstNI CCWGG 4 cut(s) 204, 284, 350, 953
BstNSI RCATGY 2 cut(s) 2350, 3699
BstSCI CCNGG 6 cut(s) 202, 282, 348, 573, 951, 1977
BstSFI CTRYAG 7 cut(s) 969, 1555, 1905, 2001, 2864, 2896, 2988
BstSLI GKGCMC 2 cut(s) 1307, 2809
BstUI CGCG 2 cut(s) 268, 2706
BstV2I GAAGAC 3 cut(s) 444, 724, 1542
BstX2I RGATCY 5 cut(s) 1583, 1603, 2311, 2860, 3334
BstXI CCANNNNNNTGG 1 cut(s) 792
BstYI RGATCY 5 cut(s) 1583, 1603, 2311, 2860, 3334
BstZ17I GTATAC 1 cut(s) 2845
BstZI CGGCCG 1 cut(s) 239
Bsu15I ATCGAT 1 cut(s) 2380
Bsu36I CCTNAGG 1 cut(s) 2495
BsuI GTATCC 1 cut(s) 1407
BsuRI GGCC 5 cut(s) 144, 241, 1515, 3384, 3790
BsuTUI ATCGAT 1 cut(s) 2380
BtgI CCRYGG 2 cut(s) 3093, 3231
BtsCI GGATG 4 cut(s) 13, 922, 2092, 3037
BtsI GCAGTG 2 cut(s) 174, 684
Cac8I GCNNGC 9 cut(s) 239, 949, 1121, 1297, 1681, 1888, 1989, 2502, 3382
CaiI CAGNNNCTG 1 cut(s) 2009
CciI TCATGA 1 cut(s) 1527
CfoI GCGC 1 cut(s) 2037
Cfr10I RCCGGY 1 cut(s) 237
Cfr13I GGNCC 3 cut(s) 1513, 3525, 3789
ClaI ATCGAT 1 cut(s) 2380
CseI GACGC 1 cut(s) 321
DraI TTTAAA 1 cut(s) 1069
DraIII CACNNNGTG 1 cut(s) 1137
EaeI YGGCCR 3 cut(s) 142, 239, 3382
EagI CGGCCG 1 cut(s) 239
Eam1104I CTCTTC 2 cut(s) 70, 3464
EarI CTCTTC 2 cut(s) 70, 3464
EciI GGCGGA 2 cut(s) 612, 3044
Ecl136II GAGCTC 1 cut(s) 1810
EclXI CGGCCG 1 cut(s) 239
Eco130I CCWWGG 2 cut(s) 145, 3093
Eco24I GRGCYC 2 cut(s) 1812, 3854
Eco32I GATATC 1 cut(s) 3799
Eco47I GGWCC 1 cut(s) 3525
Eco52I CGGCCG 1 cut(s) 239
Eco53kI GAGCTC 1 cut(s) 1810
Eco57I CTGAAG 4 cut(s) 842, 948, 1145, 1809
Eco72I CACGTG 1 cut(s) 1308
Eco81I CCTNAGG 1 cut(s) 2495
EcoICRI GAGCTC 1 cut(s) 1810
EcoRII CCWGG 4 cut(s) 202, 282, 348, 951
EcoRV GATATC 1 cut(s) 3799
EcoT14I CCWWGG 2 cut(s) 145, 3093
EcoT38I GRGCYC 2 cut(s) 1812, 3854
ErhI CCWWGG 2 cut(s) 145, 3093
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FaqI GGGAC 6 cut(s) 319, 329, 1657, 2058, 2476, 3111
FauI CCCGC 2 cut(s) 1686, 2972
FauNDI CATATG 3 cut(s) 1545, 3503, 3628
FbaI TGATCA 1 cut(s) 1723
FblI GTMKAC 1 cut(s) 2844
FokI GGATG 3 cut(s) 929, 2099, 3044
FriOI GRGCYC 2 cut(s) 1812, 3854
FspBI CTAG 9 cut(s) 71, 360, 519, 596, 882, 1764, 1917, 1994, 3810
GlaI GCGC 1 cut(s) 2036
GsuI CTGGAG 1 cut(s) 2550
HaeIII GGCC 5 cut(s) 144, 241, 1515, 3384, 3790
HapII CCGG 7 cut(s) 32, 221, 238, 574, 1354, 1979, 3873
HgaI GACGC 1 cut(s) 321
HhaI GCGC 1 cut(s) 2037
Hin6I GCGC 1 cut(s) 2035
HinP1I GCGC 1 cut(s) 2035
HincII GTYRAC 2 cut(s) 2580, 3258
HindII GTYRAC 2 cut(s) 2580, 3258
HindIII AAGCTT 2 cut(s) 1382, 1778
HpaII CCGG 7 cut(s) 32, 221, 238, 574, 1354, 1979, 3873
HpyAV CCTTC 6 cut(s) 550, 773, 1215, 1220, 1748, 2786
HpyCH4III ACNGT 9 cut(s) 649, 1095, 1556, 1897, 2114, 2337, 2577, 2837, 3102
HpyCH4IV ACGT 5 cut(s) 1307, 1340, 1877, 2395, 3296
HpyF10VI GCNNNNNNNGC 8 cut(s) 19, 55, 1680, 2773, 2813, 2872, 3077, 3815
HpySE526I ACGT 5 cut(s) 1307, 1340, 1877, 2395, 3296
HspAI GCGC 1 cut(s) 2035
KroI GCCGGC 1 cut(s) 237
KroNI GCCGGC 1 cut(s) 239
Ksp22I TGATCA 1 cut(s) 1723
LmnI GCTCC 6 cut(s) 187, 358, 1704, 2872, 3507, 3753
LweI GCATC 8 cut(s) 22, 1007, 2347, 2638, 2737, 3089, 3198, 3417
MaeI CTAG 9 cut(s) 71, 360, 519, 596, 882, 1764, 1917, 1994, 3810
MaeII ACGT 5 cut(s) 1307, 1340, 1877, 2395, 3296
MaeIII GTNAC 6 cut(s) 395, 682, 875, 1996, 2736, 3354
MbiI CCGCTC 1 cut(s) 6
MfeI CAATTG 6 cut(s) 600, 1476, 1727, 2055, 2448, 2556
MflI RGATCY 5 cut(s) 1583, 1603, 2311, 2860, 3334
MhlI GDGCHC 5 cut(s) 1307, 1812, 2809, 3494, 3854
MlsI TGGCCA 2 cut(s) 144, 3384
MluNI TGGCCA 2 cut(s) 144, 3384
MlyI GAGTC 6 cut(s) 403, 761, 932, 2210, 2498, 3457
MmeI TCCRAC 5 cut(s) 234, 2064, 2445, 2850, 3022
Mox20I TGGCCA 2 cut(s) 144, 3384
MroNI GCCGGC 1 cut(s) 237
MroXI GAANNNNTTC 2 cut(s) 557, 1361
MscI TGGCCA 2 cut(s) 144, 3384
MslI CAYNNNNRTG 1 cut(s) 2262
Msp20I TGGCCA 2 cut(s) 144, 3384
MspA1I CMGCKG 2 cut(s) 968, 1123
MspI CCGG 7 cut(s) 32, 221, 238, 574, 1354, 1979, 3873
MspR9I CCNGG 6 cut(s) 204, 284, 350, 575, 953, 1979
MunI CAATTG 6 cut(s) 600, 1476, 1727, 2055, 2448, 2556
Mva1269I GAATGC 3 cut(s) 408, 1667, 2633
MvaI CCWGG 4 cut(s) 204, 284, 350, 953
MvnI CGCG 2 cut(s) 268, 2706
MwoI GCNNNNNNNGC 8 cut(s) 19, 55, 1680, 2773, 2813, 2872, 3077, 3815
NaeI GCCGGC 1 cut(s) 239
NciI CCSGG 2 cut(s) 575, 1979
NcoI CCATGG 1 cut(s) 3093
NdeI CATATG 3 cut(s) 1545, 3503, 3628
NgoMIV GCCGGC 1 cut(s) 237
NlaIV GGNNCC 4 cut(s) 354, 1574, 1706, 1902
NmuCI GTSAC 3 cut(s) 395, 682, 875
NspI RCATGY 2 cut(s) 2350, 3699
NspV TTCGAA 1 cut(s) 514
PagI TCATGA 1 cut(s) 1527
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PctI GAATGC 3 cut(s) 408, 1667, 2633
PdiI GCCGGC 1 cut(s) 239
PdmI GAANNNNTTC 2 cut(s) 557, 1361
PflMI CCANNNNNTGG 1 cut(s) 3675
PleI GAGTC 6 cut(s) 402, 760, 931, 2210, 2497, 3456
PmaCI CACGTG 1 cut(s) 1308
PmlI CACGTG 1 cut(s) 1308
PpsI GAGTC 6 cut(s) 402, 760, 931, 2210, 2497, 3456
Ppu21I YACGTR 1 cut(s) 1308
PsiI TTATAA 1 cut(s) 3731
Psp124BI GAGCTC 1 cut(s) 1812
Psp6I CCWGG 4 cut(s) 202, 282, 348, 951
PspCI CACGTG 1 cut(s) 1308
PspGI CCWGG 4 cut(s) 202, 282, 348, 951
PspN4I GGNNCC 4 cut(s) 354, 1574, 1706, 1902
PspPI GGNCC 3 cut(s) 1513, 3525, 3789
PstI CTGCAG 2 cut(s) 2005, 2868
PstNI CAGNNNCTG 1 cut(s) 2009
PsuI RGATCY 5 cut(s) 1583, 1603, 2311, 2860, 3334
PvuII CAGCTG 2 cut(s) 968, 1123
RseI CAYNNNNRTG 1 cut(s) 2262
SacI GAGCTC 1 cut(s) 1812
Sau96I GGNCC 3 cut(s) 1513, 3525, 3789
ScaI AGTACT 1 cut(s) 1915
SchI GAGTC 6 cut(s) 403, 761, 932, 2210, 2498, 3457
ScrFI CCNGG 6 cut(s) 204, 284, 350, 575, 953, 1979
SduI GDGCHC 5 cut(s) 1307, 1812, 2809, 3494, 3854
SfaNI GCATC 8 cut(s) 22, 1007, 2347, 2638, 2737, 3089, 3198, 3417
SfcI CTRYAG 7 cut(s) 969, 1555, 1905, 2001, 2864, 2896, 2988
SfuI TTCGAA 1 cut(s) 514
SinI GGWCC 1 cut(s) 3525
SmiMI CAYNNNNRTG 1 cut(s) 2262
SmlI CTYRAG 4 cut(s) 83, 1567, 1834, 2732
SmoI CTYRAG 4 cut(s) 83, 1567, 1834, 2732
SpeI ACTAGT 2 cut(s) 1916, 1993
SspI AATATT 3 cut(s) 802, 1406, 2270
SspMI CTAG 9 cut(s) 71, 360, 519, 596, 882, 1764, 1917, 1994, 3810
SstI GAGCTC 1 cut(s) 1812
StyD4I CCNGG 6 cut(s) 202, 282, 348, 573, 951, 1977
StyI CCWWGG 2 cut(s) 145, 3093
TaaI ACNGT 9 cut(s) 649, 1095, 1556, 1897, 2114, 2337, 2577, 2837, 3102
TaiI ACGT 5 cut(s) 1310, 1343, 1880, 2398, 3299
TaqI TCGA 6 cut(s) 514, 1033, 1551, 1800, 2380, 2880
TatI WGTACW 7 cut(s) 525, 1913, 1919, 3322, 3553, 3762, 3772
TauI GCSGC 3 cut(s) 61, 244, 271
TseFI GTSAC 3 cut(s) 395, 682, 875
Tsp45I GTSAC 3 cut(s) 395, 682, 875
TspGWI ACGGA 3 cut(s) 6, 240, 1148
Van91I CCANNNNNTGG 1 cut(s) 3675
VneI GTGCAC 1 cut(s) 2805
VpaK11BI GGWCC 1 cut(s) 3525
XapI RAATTY 2 cut(s) 2105, 3045
XceI RCATGY 2 cut(s) 2350, 3699
XcmI CCANNNNNNNNNTGG 1 cut(s) 3414
XmiI GTMKAC 1 cut(s) 2844
XmnI GAANNNNTTC 2 cut(s) 557, 1361
XspI CTAG 9 cut(s) 71, 360, 519, 596, 882, 1764, 1917, 1994, 3810
ZrmI AGTACT 1 cut(s) 1915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.