Rh3AG099900

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0009696
Physical Location & Seq
Forward (+)
8314075 .. 8318918
4844 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG099900.1

Sequence Viewer

Length: 3735 bp
ATGAGCGGTGAGCATCCGTTGCTGTCATCACCGGATTCTCCATCAAAGCCACTTGTGCCGCCCTCTTCTCTAGCTGAGAGACTCGAGCAGCTCTGCCTTGATGAATCCAGTTCTTCTTCCGTTCTTGACGGCATTGATGATGGCCAAGGTGAATCGTTGTTAGCGAGGGAATACACTGCTGTTCCTGACTGCGGTGTGAAACCAGGAAAGCAGTTTTTTCCTGTAGAGCGTGAGTGCCGTCCGCAGTTTCCGTTGGAATACCCCACGCGGCATAAAAAACGCCAGGTATCGTGGGGTACAATGGAACTGCATAGTATCAATAGTAATCCAGCGTCCCATGATGTCCCCCAGGCTCCATCTAGGGTTCTGGAAAAGGCGAACAAGTCTCAGGGGAGTCACCACAAAGGCATTCAATTTGAAGATAACCTCCTACATGAAGACAATCCAAGGTTGATCTATATTGATGATCCGAAGAAAACAAATGACAAGTATGAGTTCACTGGCAATGAGATTCGAACTAGCAAGTACACCTTAATCACCTTCTTGCCCAAGAATGTTTTCATTCAGTTTCACCGGGTTGCTTATTTGTATTTTCTAGCTATTGCCGTTCTCAATCAGCTTCCACCTCTTGCTGTGTTTGGAAGAACGGTGTCTCTTTTTCCCCTCCTATTTGTGCTGTGTGTCACTGCTATAAAAGATGGCTATGAGGATTGGCGAAGACATAGATCAGACAGGAATGAAAACAACCGGGAGTCTCTGATCCTTCAATCTGGGCAATTTCAACCAAAGAAATGGAAAAATATTCAAGTGGGCGAGGTTCTGAAGATTCGTGCTGATGATACAATTCCTTGTGATATAGTTTTGTTAGGAACAAGTGACCCCAGTGGAATTGCCTACATTCAGACAATGAATCTGGATGGTGAGTCAAACCTCAAAACAAGGTATGCACGCCAGGAAACATCTTCAGCTGTAGGTTCAGCAGTAGCTGATGGGTGTGCAATTATGGGGATCATCAGATGCGAACAACCAAATCGGAATATCTATGAGTTTACAGCCAACATGGAGTTTAATGGACATAGATTTCCCCTAACACAGTCAAACATAGTTTTGCGTGGTTGCCAGCTGAAGAACACGGAGTGGATAATTGGTGTTGTGGTATATGCTGGACAGGAAACAAAGGCAATGTTGAATAGTGCAGCTTCTCCTTCCAAGAGAAGCAAACTTGAAGGCTACATGAACAGAGAAACCTTGTGGCTGTCAATTTTCCTTTTTGTCATGTGTTCAGTTGTGGCTGCTGGCATGGGCACGTGGCTAATACGCCATAAATATCAGGTTGATACGTTGCCTTATTACCGGAAAAGATTCTTCACTAATTGGGGGAAGCTTAATGGGAAAACCTATAAATATTATGGGATACCTATGGAGATTTTTTTCTCCTTTTTGAGTTCTGTCATAGTGTTTCAGATCATGATACCAATCGCTCTGTATATTAGTATTGAGTTGGTTCGTTTGGGCCAGTCATATTTCATGATTGAAGACAGGCATATGTTCGACTGTAGCTCTGGCTCAAGGTTCCAGTGCAGATCCTTGAATATCAATGAGGATCTGGGTCAAATACGATATATATTTTCAGACAAAACAGGGACACTTACTGAAAATAAGATGGCATTCCGCAGGGCAAGCATATATGGGAGGGATTATGGGAGCCGTGTGCTTGTGGCTGATCAATTGCAGGAAGAAAATAATACAGGAGAAGGTTTAGCTAGAAAGAGATGGAAGCTTAAATCTGAAGTTGCTGTCGATTATGAGCTCATGGAGTTGTTGCACAAAGACTTGAGTGAAGATGAGAGGATTGCTGCACATGAGTTTTTCCTTACGTTGGCAGCTTGCAATACTGTGGTTCCTATAGTCAGTACTAGTACATCTTCCAGTTGTGCTAAGGGTGATGTTGATTCTATAGATTATCAGGGGGAATCCCCGGACGAGCAAGCACTAGTTACTGCAGCCTCTGGTTATAGATATACTCTCTTTGAGCGCACATCGGGACATATTGCAATTGATGTCAATGGTGAGAAACTAAGGTTGGATGTATTGGGTCTGCATGAATTTGACAGTGTGCGAAAACGAATGTCAGTTGTTATCAGATTTCCAAACAATACTATCAAGGTATTGGTGAAAGGTGCTGATACCTCTATGCTCAGCATATTAGCGAATGACTCTGAAAGAGATGATGAACTGAGACATTCAACTCAGAGCCATTTGAGTGAATATGCCTCACATGGTTTACGTACTCTTGTGATTGCTGCAAGAGATCTTACAAATGAAGAACTTGAACAGTGGCAAAGCATGTATGAAGATGCAAGTACCTCATTGACTGATCGATCCTTAAAACTACGTCAAACAGCAGCTCTTATTGAATCCAACTTAAAGCTACTTGGGGCAACTGCAATTGAGGATAGGCTACAAGATGGTGTGCCAGAAGCTATTGAGTCCCTCAGGCAAGCAGGAATTAAGGTCTGGGTTTTGACTGGAGATAAGCAAGAGACAGCTATTTCAATTGGTGTATCATGCAAACTGTTGACAGCAGATATGCAACAAATTATCATAAATGGAACTTCTGAGGCTGAATGCAGAAATCTTTTGGTTGATGCTATGGAAAAATATGGTGTGCAGTCATCAAATAAAAGAAATCAGAGTTCAAAATGCAAAAGCAATGCTGCAAGTGATTATCTTGAGTTACCTGATGATGCAAAGACATCTAATGTGCCAAAATGGCATGAAGCAAAGGAAGTAGGAAAGATAAGTGCACCATTAGCACTCATAATTGATGGGAACAGTTTGGTATACATTTTGGAGAAAGATCTGCAGTCGGAGCTTTTCGACCTTGCCACTTCCTGTAGTGTGGTTGTATGCTGTCGTGTTGCACCATTGCAGAAAGCTGGAATTGTTGATCTAATTAAGACCCGCACTGATGATATGACACTGGCTATAGGTGATGGGGCAAATGATGTTTCTATGATCCAAATGGCGGATGTTGGTGTTGGAATTTGTGTTTTTGTGCTGATGCTATTTTGGTATATATTGTCCACAGCATTTTCAACAACTTCGGCGTTGACAGATTGGAGTAGTGTTTTCTATTCTCTTATATATACGTCTATCCCTACAATTATCGTTGGTGTACTGGACAAAGATCTGAGCCACAAGACCCTGTTACAATACCCAAAATTGTATGGTGCTGGCCATAGACAGGAAGCATATAATCCAAATCTTTTCTGGATAACGATGCTTGACACCCTATGGCAGAGTCTTGTTCTCTTCTATGTACCCCAGTTCACCTATAAGGAGAGCACAATAGACATATGGAGCATGGGCAGTTTGTGGACCATAGCAGTAGTTATCCTTGTTAATGTACATTTGGCAATGGACATTCATCGGTGGGTATTTATCACTCATATTGCAGTATGGGGATCAATAATCATCACATATGCCTGTGTGGTGATATTGGATTCCATACCTGTTTATCCCAATTACTGGACTTTTTACCATTTGGCATGTTCTCCTACCTATTGGATCACCATTTTGCTTATAATAGTTGTAGCCTTGCTCCCTCGTTTTGTGTACAAAGTTGTTCATCATATTTTTTGGCCCTCAGATATCCAGATAGCTAGAGAAGGTGAGATATTAAGTAGGAAACATAAGCATTTGGGCTCAGAAAAAGATGAAGATTCAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1244

Amino Acids

140.18

Weight (kDa)

5.93

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhoLip_ATPase_N PF16209 152 - 217 3.9e-22 Phospholipid-translocating ATPase N-terminal
Cation_ATPase PF13246 658 - 822 1.4e-14 P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C PF16212 1018 - 1217 1.5e-52 Phospholipid-translocating P-type ATPase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3587
AccB7I CCANNNNNTGG 1 cut(s) 3531
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 2844
AccII CGCG 1 cut(s) 268
AciI CCGC 8 cut(s) 6, 59, 192, 242, 268, 1672, 2965, 3029
AclWI GGATC 9 cut(s) 461, 754, 1016, 1578, 1611, 2376, 3013, 3475, 3578
AcoI YGGCCR 2 cut(s) 142, 3238
AcsI RAATTY 2 cut(s) 2105, 3045
AcuI CTGAAG 4 cut(s) 842, 948, 1145, 1809
AcvI CACGTG 1 cut(s) 1308
AdeI CACNNNGTG 1 cut(s) 1137
AfiI CCNNNNNNNGG 5 cut(s) 191, 1879, 3028, 3247, 3531
AhlI ACTAGT 2 cut(s) 1916, 1993
AjnI CCWGG 4 cut(s) 202, 282, 348, 951
AjuI GAANNNNNNNTTGG 4 cut(s) 542, 574, 2066, 2098
Alw21I GWGCWC 3 cut(s) 1812, 2809, 3350
Alw26I GTCTC 6 cut(s) 73, 390, 657, 759, 2233, 2537
Alw44I GTGCAC 1 cut(s) 2805
AlwI GGATC 9 cut(s) 461, 754, 1016, 1578, 1611, 2376, 3013, 3475, 3578
AlwNI CAGNNNCTG 2 cut(s) 986, 2009
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 4 cut(s) 142, 1513, 3238, 3644
ApaLI GTGCAC 1 cut(s) 2805
ApeKI GCWGC 9 cut(s) 88, 1196, 1292, 1856, 1883, 2003, 2303, 2405, 2717
ApoI RAATTY 2 cut(s) 2105, 3045
Asp700I GAANNNNTTC 2 cut(s) 557, 1361
AspLEI GCGC 1 cut(s) 2037
AspS9I GGNCC 3 cut(s) 1513, 3381, 3645
AsuC2I CCSGG 3 cut(s) 575, 749, 1979
AsuII TTCGAA 1 cut(s) 514
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 1 cut(s) 3381
AxyI CCTNAGG 1 cut(s) 2495
BaeGI GKGCMC 2 cut(s) 1307, 2809
BalI TGGCCA 2 cut(s) 144, 3240
BanII GRGCYC 2 cut(s) 1812, 3710
BbrPI CACGTG 1 cut(s) 1308
BbsI GAAGAC 3 cut(s) 444, 724, 1542
Bbv12I GWGCWC 3 cut(s) 1812, 2809, 3350
BbvI GCAGC 9 cut(s) 100, 1208, 1279, 1843, 1895, 2015, 2290, 2417, 2704
BceAI ACGGC 4 cut(s) 145, 222, 590, 1692
BciT130I CCWGG 4 cut(s) 204, 284, 350, 953
BciVI GTATCC 1 cut(s) 1407
BclI TGATCA 1 cut(s) 1723
BcnI CCSGG 3 cut(s) 575, 749, 1979
BcoDI GTCTC 6 cut(s) 73, 390, 657, 759, 2233, 2537
BcuI ACTAGT 2 cut(s) 1916, 1993
BfaI CTAG 8 cut(s) 71, 360, 519, 596, 1764, 1917, 1994, 3666
BfmI CTRYAG 9 cut(s) 222, 969, 1555, 1905, 1956, 2001, 2864, 2896, 2988
BfuI GTATCC 1 cut(s) 1407
BglI GCCNNNNNGGC 1 cut(s) 2773
BglII AGATCT 3 cut(s) 2311, 2860, 3190
BlpI GCTNAGC 1 cut(s) 2198
BmcAI AGTACT 1 cut(s) 1915
Bme1390I CCNGG 7 cut(s) 204, 284, 350, 575, 749, 953, 1979
Bme18I GGWCC 1 cut(s) 3381
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 3 cut(s) 1513, 3381, 3645
BmiI GGNNCC 4 cut(s) 354, 1574, 1706, 1902
BmrFI CCNGG 7 cut(s) 204, 284, 350, 575, 749, 953, 1979
BmrI ACTGGG 2 cut(s) 876, 3322
BmsI GCATC 7 cut(s) 22, 1007, 2347, 2638, 2737, 3054, 3273
BmuI ACTGGG 2 cut(s) 876, 3322
BpiI GAAGAC 3 cut(s) 444, 724, 1542
BpmI CTGGAG 1 cut(s) 2550
Bpu10I CCTNAGC 1 cut(s) 1938
Bpu1102I GCTNAGC 1 cut(s) 2198
Bpu14I TTCGAA 1 cut(s) 514
BpuEI CTTGAG 3 cut(s) 1552, 1855, 2753
BpuMI CCSGG 3 cut(s) 575, 749, 1979
Bsa29I ATCGAT 1 cut(s) 2380
BsaAI YACGTR 2 cut(s) 1308, 2288
BsaBI GATNNNNATC 1 cut(s) 1475
BsaJI CCNNGG 4 cut(s) 145, 348, 446, 1977
BsaWI WCCGGW 2 cut(s) 31, 1353
Bsc4I CCNNNNNNNGG 5 cut(s) 191, 1879, 3028, 3247, 3531
Bse21I CCTNAGG 1 cut(s) 2495
Bse3DI GCAATG 5 cut(s) 511, 1188, 2719, 2927, 3426
Bse8I GATNNNNATC 1 cut(s) 1475
BseBI CCWGG 4 cut(s) 204, 284, 350, 953
BseCI ATCGAT 1 cut(s) 2380
BseDI CCNNGG 4 cut(s) 145, 348, 446, 1977
BseGI GGATG 4 cut(s) 13, 922, 2092, 3037
BseJI GATNNNNATC 1 cut(s) 1475
BseLI CCNNNNNNNGG 5 cut(s) 191, 1879, 3028, 3247, 3531
BseMI GCAATG 5 cut(s) 511, 1188, 2719, 2927, 3426
BseSI GKGCMC 2 cut(s) 1307, 2809
BseXI GCAGC 9 cut(s) 100, 1208, 1279, 1843, 1895, 2015, 2290, 2417, 2704
BsgI GTGCAG 4 cut(s) 1215, 1600, 1842, 2690
Bsh1236I CGCG 1 cut(s) 268
BshFI GGCC 4 cut(s) 144, 1515, 3240, 3646
BshVI ATCGAT 1 cut(s) 2380
BsiHKAI GWGCWC 3 cut(s) 1812, 2809, 3350
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 5 cut(s) 32, 574, 748, 1354, 1979
BslFI GGGAC 5 cut(s) 319, 329, 1657, 2058, 2476
BslI CCNNNNNNNGG 5 cut(s) 191, 1879, 3028, 3247, 3531
BsmAI GTCTC 6 cut(s) 73, 390, 657, 759, 2233, 2537
BsmFI GGGAC 5 cut(s) 319, 329, 1657, 2058, 2476
BsmI GAATGC 3 cut(s) 408, 1667, 2633
BsnI GGCC 4 cut(s) 144, 1515, 3240, 3646
BsoBI CYCGRG 1 cut(s) 83
Bsp119I TTCGAA 1 cut(s) 514
Bsp1286I GDGCHC 5 cut(s) 1307, 1812, 2809, 3350, 3710
Bsp1407I TGTACA 2 cut(s) 3409, 3618
Bsp1720I GCTNAGC 1 cut(s) 2198
BspACI CCGC 8 cut(s) 6, 59, 192, 242, 268, 1672, 2965, 3029
BspANI GGCC 4 cut(s) 144, 1515, 3240, 3646
BspDI ATCGAT 1 cut(s) 2380
BspFNI CGCG 1 cut(s) 268
BspHI TCATGA 2 cut(s) 1467, 1527
BspLI GGNNCC 4 cut(s) 354, 1574, 1706, 1902
BspMAI CTGCAG 2 cut(s) 2005, 2868
BspPI GGATC 9 cut(s) 461, 754, 1016, 1578, 1611, 2376, 3013, 3475, 3578
BspT104I TTCGAA 1 cut(s) 514
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 5 cut(s) 511, 1188, 2719, 2927, 3426
BsrGI TGTACA 2 cut(s) 3409, 3618
BssECI CCNNGG 4 cut(s) 145, 348, 446, 1977
BssNAI GTATAC 1 cut(s) 2845
BssT1I CCWWGG 2 cut(s) 145, 446
Bst1107I GTATAC 1 cut(s) 2845
Bst2UI CCWGG 4 cut(s) 204, 284, 350, 953
Bst4CI ACNGT 8 cut(s) 649, 1095, 1556, 1897, 2114, 2337, 2577, 2837
Bst6I CTCTTC 2 cut(s) 70, 3320
BstAPI GCANNNNNTGC 1 cut(s) 19
BstAUI TGTACA 2 cut(s) 3409, 3618
BstBAI YACGTR 2 cut(s) 1308, 2288
BstBI TTCGAA 1 cut(s) 514
BstC8I GCNNGC 8 cut(s) 949, 1121, 1297, 1681, 1888, 1989, 2502, 3238
BstF5I GGATG 4 cut(s) 13, 922, 2092, 3037
BstFNI CGCG 1 cut(s) 268
BstHHI GCGC 1 cut(s) 2037
BstMAI GTCTC 6 cut(s) 73, 390, 657, 759, 2233, 2537
BstMWI GCNNNNNNNGC 6 cut(s) 19, 55, 1680, 2773, 2813, 2872
BstNI CCWGG 4 cut(s) 204, 284, 350, 953
BstNSI RCATGY 2 cut(s) 2350, 3555
BstSCI CCNGG 7 cut(s) 202, 282, 348, 573, 747, 951, 1977
BstSFI CTRYAG 9 cut(s) 222, 969, 1555, 1905, 1956, 2001, 2864, 2896, 2988
BstSLI GKGCMC 2 cut(s) 1307, 2809
BstSNI TACGTA 1 cut(s) 2288
BstUI CGCG 1 cut(s) 268
BstV1I GCAGC 9 cut(s) 100, 1208, 1279, 1843, 1895, 2015, 2290, 2417, 2704
BstV2I GAAGAC 3 cut(s) 444, 724, 1542
BstX2I RGATCY 5 cut(s) 1583, 1603, 2311, 2860, 3190
BstXI CCANNNNNNTGG 1 cut(s) 792
BstYI RGATCY 5 cut(s) 1583, 1603, 2311, 2860, 3190
BstZ17I GTATAC 1 cut(s) 2845
Bsu15I ATCGAT 1 cut(s) 2380
Bsu36I CCTNAGG 1 cut(s) 2495
BsuI GTATCC 1 cut(s) 1407
BsuRI GGCC 4 cut(s) 144, 1515, 3240, 3646
BsuTUI ATCGAT 1 cut(s) 2380
BtsCI GGATG 4 cut(s) 13, 922, 2092, 3037
BtsI GCAGTG 2 cut(s) 174, 684
BtsIMutI CAGTG 9 cut(s) 174, 498, 684, 889, 1583, 2119, 2342, 2967, 2981
Cac8I GCNNGC 8 cut(s) 949, 1121, 1297, 1681, 1888, 1989, 2502, 3238
CaiI CAGNNNCTG 2 cut(s) 986, 2009
CciI TCATGA 2 cut(s) 1467, 1527
CfoI GCGC 1 cut(s) 2037
Cfr13I GGNCC 3 cut(s) 1513, 3381, 3645
ClaI ATCGAT 1 cut(s) 2380
CseI GACGC 1 cut(s) 321
DraIII CACNNNGTG 1 cut(s) 1137
EaeI YGGCCR 2 cut(s) 142, 3238
Eam1104I CTCTTC 2 cut(s) 70, 3320
EarI CTCTTC 2 cut(s) 70, 3320
EciI GGCGGA 1 cut(s) 3044
Ecl136II GAGCTC 1 cut(s) 1810
Eco105I TACGTA 1 cut(s) 2288
Eco130I CCWWGG 2 cut(s) 145, 446
Eco24I GRGCYC 2 cut(s) 1812, 3710
Eco32I GATATC 1 cut(s) 3655
Eco47I GGWCC 1 cut(s) 3381
Eco53kI GAGCTC 1 cut(s) 1810
Eco57I CTGAAG 4 cut(s) 842, 948, 1145, 1809
Eco72I CACGTG 1 cut(s) 1308
Eco81I CCTNAGG 1 cut(s) 2495
Eco88I CYCGRG 1 cut(s) 83
EcoICRI GAGCTC 1 cut(s) 1810
EcoRII CCWGG 4 cut(s) 202, 282, 348, 951
EcoRV GATATC 1 cut(s) 3655
EcoT14I CCWWGG 2 cut(s) 145, 446
EcoT38I GRGCYC 2 cut(s) 1812, 3710
ErhI CCWWGG 2 cut(s) 145, 446
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FaqI GGGAC 5 cut(s) 319, 329, 1657, 2058, 2476
FauI CCCGC 1 cut(s) 2972
FauNDI CATATG 3 cut(s) 1545, 3359, 3484
FbaI TGATCA 1 cut(s) 1723
FblI GTMKAC 1 cut(s) 2844
FokI GGATG 3 cut(s) 929, 2099, 3044
FriOI GRGCYC 2 cut(s) 1812, 3710
FspBI CTAG 8 cut(s) 71, 360, 519, 596, 1764, 1917, 1994, 3666
GlaI GCGC 1 cut(s) 2036
GsuI CTGGAG 1 cut(s) 2550
HaeIII GGCC 4 cut(s) 144, 1515, 3240, 3646
HapII CCGG 5 cut(s) 32, 574, 748, 1354, 1979
HgaI GACGC 1 cut(s) 321
HhaI GCGC 1 cut(s) 2037
Hin6I GCGC 1 cut(s) 2035
HinP1I GCGC 1 cut(s) 2035
HincII GTYRAC 2 cut(s) 2580, 3114
HindII GTYRAC 2 cut(s) 2580, 3114
HindIII AAGCTT 2 cut(s) 1382, 1778
HpaII CCGG 5 cut(s) 32, 574, 748, 1354, 1979
HpyAV CCTTC 6 cut(s) 550, 773, 1215, 1220, 1748, 3665
HpyCH4III ACNGT 8 cut(s) 649, 1095, 1556, 1897, 2114, 2337, 2577, 2837
HpyCH4IV ACGT 6 cut(s) 1307, 1340, 1877, 2287, 2395, 3152
HpyF10VI GCNNNNNNNGC 6 cut(s) 19, 55, 1680, 2773, 2813, 2872
HpySE526I ACGT 6 cut(s) 1307, 1340, 1877, 2287, 2395, 3152
HspAI GCGC 1 cut(s) 2035
Ksp22I TGATCA 1 cut(s) 1723
LmnI GCTCC 5 cut(s) 358, 1704, 2872, 3363, 3609
Lsp1109I GCAGC 9 cut(s) 100, 1208, 1279, 1843, 1895, 2015, 2290, 2417, 2704
LweI GCATC 7 cut(s) 22, 1007, 2347, 2638, 2737, 3054, 3273
MaeI CTAG 8 cut(s) 71, 360, 519, 596, 1764, 1917, 1994, 3666
MaeII ACGT 6 cut(s) 1307, 1340, 1877, 2287, 2395, 3152
MaeIII GTNAC 6 cut(s) 395, 682, 875, 1996, 2736, 3210
MbiI CCGCTC 1 cut(s) 6
MfeI CAATTG 4 cut(s) 1727, 2055, 2448, 2556
MflI RGATCY 5 cut(s) 1583, 1603, 2311, 2860, 3190
MhlI GDGCHC 5 cut(s) 1307, 1812, 2809, 3350, 3710
MlsI TGGCCA 2 cut(s) 144, 3240
MluNI TGGCCA 2 cut(s) 144, 3240
MlyI GAGTC 7 cut(s) 75, 403, 761, 932, 2210, 2498, 3313
MmeI TCCRAC 5 cut(s) 234, 2064, 2445, 2850, 3022
Mox20I TGGCCA 2 cut(s) 144, 3240
MroXI GAANNNNTTC 2 cut(s) 557, 1361
MscI TGGCCA 2 cut(s) 144, 3240
MslI CAYNNNNRTG 1 cut(s) 2262
Msp20I TGGCCA 2 cut(s) 144, 3240
MspA1I CMGCKG 2 cut(s) 968, 1123
MspI CCGG 5 cut(s) 32, 574, 748, 1354, 1979
MspR9I CCNGG 7 cut(s) 204, 284, 350, 575, 749, 953, 1979
MunI CAATTG 4 cut(s) 1727, 2055, 2448, 2556
Mva1269I GAATGC 3 cut(s) 408, 1667, 2633
MvaI CCWGG 4 cut(s) 204, 284, 350, 953
MvnI CGCG 1 cut(s) 268
MwoI GCNNNNNNNGC 6 cut(s) 19, 55, 1680, 2773, 2813, 2872
NciI CCSGG 3 cut(s) 575, 749, 1979
NdeI CATATG 3 cut(s) 1545, 3359, 3484
NlaIV GGNNCC 4 cut(s) 354, 1574, 1706, 1902
NmuCI GTSAC 3 cut(s) 395, 682, 875
NspI RCATGY 2 cut(s) 2350, 3555
NspV TTCGAA 1 cut(s) 514
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 2 cut(s) 1467, 1527
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PctI GAATGC 3 cut(s) 408, 1667, 2633
PdmI GAANNNNTTC 2 cut(s) 557, 1361
PflMI CCANNNNNTGG 1 cut(s) 3531
PleI GAGTC 7 cut(s) 75, 402, 760, 931, 2210, 2497, 3312
PmaCI CACGTG 1 cut(s) 1308
PmlI CACGTG 1 cut(s) 1308
PpsI GAGTC 7 cut(s) 75, 402, 760, 931, 2210, 2497, 3312
Ppu21I YACGTR 2 cut(s) 1308, 2288
PsiI TTATAA 1 cut(s) 3587
Psp124BI GAGCTC 1 cut(s) 1812
Psp6I CCWGG 4 cut(s) 202, 282, 348, 951
PspCI CACGTG 1 cut(s) 1308
PspGI CCWGG 4 cut(s) 202, 282, 348, 951
PspN4I GGNNCC 4 cut(s) 354, 1574, 1706, 1902
PspPI GGNCC 3 cut(s) 1513, 3381, 3645
PspXI VCTCGAGB 1 cut(s) 83
PstI CTGCAG 2 cut(s) 2005, 2868
PstNI CAGNNNCTG 2 cut(s) 986, 2009
PsuI RGATCY 5 cut(s) 1583, 1603, 2311, 2860, 3190
PvuII CAGCTG 2 cut(s) 968, 1123
RseI CAYNNNNRTG 1 cut(s) 2262
SacI GAGCTC 1 cut(s) 1812
Sau96I GGNCC 3 cut(s) 1513, 3381, 3645
ScaI AGTACT 1 cut(s) 1915
SchI GAGTC 7 cut(s) 75, 403, 761, 932, 2210, 2498, 3313
ScrFI CCNGG 7 cut(s) 204, 284, 350, 575, 749, 953, 1979
SduI GDGCHC 5 cut(s) 1307, 1812, 2809, 3350, 3710
SfaNI GCATC 7 cut(s) 22, 1007, 2347, 2638, 2737, 3054, 3273
SfcI CTRYAG 9 cut(s) 222, 969, 1555, 1905, 1956, 2001, 2864, 2896, 2988
Sfr274I CTCGAG 1 cut(s) 83
SfuI TTCGAA 1 cut(s) 514
SinI GGWCC 1 cut(s) 3381
SlaI CTCGAG 1 cut(s) 83
SmiMI CAYNNNNRTG 1 cut(s) 2262
SmlI CTYRAG 4 cut(s) 83, 1567, 1834, 2732
SmoI CTYRAG 4 cut(s) 83, 1567, 1834, 2732
SnaBI TACGTA 1 cut(s) 2288
SpeI ACTAGT 2 cut(s) 1916, 1993
SsiI CCGC 8 cut(s) 6, 59, 192, 242, 268, 1672, 2965, 3029
SspI AATATT 2 cut(s) 802, 1406
SspMI CTAG 8 cut(s) 71, 360, 519, 596, 1764, 1917, 1994, 3666
SstI GAGCTC 1 cut(s) 1812
StyD4I CCNGG 7 cut(s) 202, 282, 348, 573, 747, 951, 1977
StyI CCWWGG 2 cut(s) 145, 446
TaaI ACNGT 8 cut(s) 649, 1095, 1556, 1897, 2114, 2337, 2577, 2837
TaiI ACGT 6 cut(s) 1310, 1343, 1880, 2290, 2398, 3155
TaqI TCGA 6 cut(s) 84, 514, 1551, 1800, 2380, 2880
TatI WGTACW 6 cut(s) 525, 1913, 1919, 3178, 3409, 3618
TauI GCSGC 2 cut(s) 61, 271
TscAI CASTG 9 cut(s) 181, 505, 691, 889, 1583, 2119, 2342, 2974, 2988
TseFI GTSAC 3 cut(s) 395, 682, 875
TseI GCWGC 9 cut(s) 88, 1196, 1292, 1856, 1883, 2003, 2303, 2405, 2717
Tsp45I GTSAC 3 cut(s) 395, 682, 875
TspGWI ACGGA 4 cut(s) 6, 109, 240, 1148
TspRI CASTG 9 cut(s) 181, 505, 691, 889, 1583, 2119, 2342, 2974, 2988
Van91I CCANNNNNTGG 1 cut(s) 3531
VneI GTGCAC 1 cut(s) 2805
VpaK11BI GGWCC 1 cut(s) 3381
XapI RAATTY 2 cut(s) 2105, 3045
XceI RCATGY 2 cut(s) 2350, 3555
XcmI CCANNNNNNNNNTGG 1 cut(s) 3270
XhoI CTCGAG 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 2844
XmnI GAANNNNTTC 2 cut(s) 557, 1361
XspI CTAG 8 cut(s) 71, 360, 519, 596, 1764, 1917, 1994, 3666
ZrmI AGTACT 1 cut(s) 1915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.