Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0010322

Physical Location & Seq

Genomic Coordinates Reverse (-)

6333426 .. 6336523

Gene Structure 3098 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g10580.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1242 bp

ATGGACTCCTCATCTCCACTCCCACTCCAGCAAACCCCTCTGGCCTCCCCAAAGCCCTCCAAGAAAAGCTTCGTCTCTTCTCTAATGAACGTCGCCGCTCTCCGAACACATTCTTTCAAGGAAGACACCTACTTTGTCTCCACCCTCAAGTCCTCGGAGAAGAAGGCTCTCCAGGAGCTCAAAGACAAGCTCATTGCCTCGGAAGCCTGCGACGCCGCCATGTGGGGCGTCAAGCTGCTCGCCGGAGACGACAAGGCCGACGTCATCCTCCTCAAGTTTCTCCGGGCGAGGGACTTCCGTGTTGCCGAGGCTTTCAACATGCTAGTCAAATGTCTTGAATGGAGGAAGGAGTTTGGGGCTGATGGGGTTGTTGAGGAGGACTTGGGATTTAAGGAGCTTGAAGGTGTGGTGGCGTATATGCATGGGTTTGATAGGTGTGGGCATCCGGTGTGTTATAATGCTTATGGAATTTTTAAGGATAAGGACATGTATGAAAGGATTTTTGGGGACGAGGAGAAGCTTAAGAAGTTCTTGAGGTGGAGAGTTCAGGTTCTTGAGAAGGGTATCAATGTCTTGCATTTTAAGCCTGGAGGGATCAACTCCATTATTCAAGTCACTGATCTTAAGGACATGCCCAAAAGAGAGCTAAGGGTTGCTTCGAATCAGATCCTCTCGCTGTTTCAGGACAACTACCCAGAAATGGTGGCCAGAAAGATTTTCATCAATGTACCGTGGTACTTCAGTGTTCTGTATTCGATGTTCAGTCCGTTTCTGACCCAAAGAACTAAGAGCAAGTTTGTGATATCCAAGGAAGGAAATGTTGCAGAGACACTCTACAAATTTATTAGGCCTGAGGATGTTCCAGTTCAGTATGGTGGACTGAGTCGACCCAATGATTTGCAAAACGGTCCTCCCAAACCGGCTTCGGAATTCACTGTCAAAGGAGGAGAGAAAGTTAACATTCAGATCGAGGGGATTGAGGCTGGTGCAACAATTACATGGGACATAGTTGTTGGAGGGTGGGACTTGGAGTACAGTGCTGAGTTTGTGCCAAGTTCACAAGGAAGCTACATTGTTGCCGTGGAGAAGCCGAGAAAAGTCGCACCATCTGAAGAAGCGCTCCGCAACTCCTTTACTCCAAAAGAGGCCGGAAAAATGGTATTCTCAGTGGACAATTCTGCTTCCCGGAAAAAGAAGGTCGCTGCGTACCGGTACATTGTCCGGAAATCTGCCTCTGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRAL_TRIO CRAL_TRIO_N

Protein Analysis

414

Amino Acids

46.52

Weight (kDa)

9.01

Isoelectric Point (pI)

37.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRAL_TRIO_N	PF03765	56 - 110	2.1e-12	CRAL/TRIO, N-terminal domain
CRAL_TRIO	PF00650	134 - 293	1.6e-31	CRAL/TRIO domain
GOLD_PATL1_C	PF25099	314 - 398	1.2e-29	Patellin-1-like, C-terminal GOLD domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010322)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51670
fragaria_vesca	FvH4_6g10580
malus_domestica	MD04G1167000.v1.1 MD12G1180600.v1.1
prunus_persica	Prupe.6G285300_v2.0.a1 Prupe.6G285300_v2.0.a1
pyrus_communis	pycom04g14840 pycom12g17020
rosa_chinensis	RchiOBHm_Chr3g0461561
rosa_laevigata	RLG00000014119 RLG00000024918
rosa_multiflora	Rmu_co8289033.1_g000001 Rmu_ssc0000193.1_g000001
rosa_roxburghii	Rroxscaffold_6G00418140 Rroxscaffold_6G00418510
rosa_rugosa	Rorug03G0051900 Rorug06G0023600
rosa_samantha	Rh3AG110000 Rh3BG113300 Rh3DG114900
rosa_wichuraiana	Rw3G009300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	456
AatII	GACGTC	1 cut(s)	264
AccBSI	CCGCTC	1 cut(s)	98
AccI	GTMKAC	1 cut(s)	886
AccIII	TCCGGA	1 cut(s)	1221
AciI	CCGC	3 cut(s)	96, 216, 1123
AclWI	GGATC	2 cut(s)	602, 661
AcoI	YGGCCR	1 cut(s)	705
AcsI	RAATTY	3 cut(s)	468, 839, 929
AcuI	CTGAAG	2 cut(s)	724, 1131
AcyI	GRCGYC	3 cut(s)	213, 228, 261
AfaI	GTAC	5 cut(s)	729, 737, 1034, 1208, 1214
AfeI	AGCGCT	1 cut(s)	1119
AfiI	CCNNNNNNNGG	3 cut(s)	222, 289, 700
AflII	CTTAAG	2 cut(s)	521, 623
AflIII	ACRYGT	1 cut(s)	486
AgeI	ACCGGT	1 cut(s)	1209
AgsI	TTSAA	5 cut(s)	118, 316, 338, 401, 611
AjnI	CCWGG	2 cut(s)	171, 586
AjuI	GAANNNNNNNTTGG	4 cut(s)	53, 85, 486, 518
AluBI	AGCT	8 cut(s)	69, 178, 190, 235, 397, 520, 646, 1068
AluI	AGCT	8 cut(s)	69, 178, 190, 235, 397, 520, 646, 1068
Alw21I	GWGCWC	1 cut(s)	180
Alw26I	GTCTC	4 cut(s)	79, 142, 240, 821
AlwI	GGATC	2 cut(s)	602, 661
Aor13HI	TCCGGA	1 cut(s)	1221
Aor51HI	AGCGCT	1 cut(s)	1119
AoxI	GGCC	5 cut(s)	42, 255, 705, 848, 1146
ApeKI	GCWGC	2 cut(s)	235, 1202
ApoI	RAATTY	3 cut(s)	468, 839, 929
ArsI	GACNNNNNNTTYG	2 cut(s)	116, 148
AsiGI	ACCGGT	1 cut(s)	1209
Asp700I	GAANNNNTTC	2 cut(s)	68, 109
AspLEI	GCGC	1 cut(s)	1120
AspS9I	GGNCC	1 cut(s)	908
AsuC2I	CCSGG	2 cut(s)	284, 1186
AsuII	TTCGAA	1 cut(s)	659
AvaII	GGWCC	1 cut(s)	908
AxyI	CCTNAGG	1 cut(s)	852
BaeI	ACNNNNGTAYC	2 cut(s)	727, 760
BalI	TGGCCA	1 cut(s)	707
BanII	GRGCYC	1 cut(s)	180
BbsI	GAAGAC	1 cut(s)	129
Bbv12I	GWGCWC	1 cut(s)	180
BbvI	GCAGC	2 cut(s)	222, 1189
BccI	CCATC	2 cut(s)	356, 1114
BceAI	ACGGC	1 cut(s)	1064
BciT130I	CCWGG	2 cut(s)	173, 588
BcnI	CCSGG	2 cut(s)	284, 1186
BcoDI	GTCTC	4 cut(s)	79, 142, 240, 821
BfaI	CTAG	2 cut(s)	323, 1240
BfoI	RGCGCY	1 cut(s)	1121
BfrI	CTTAAG	2 cut(s)	521, 623
BisI	GCNGC	4 cut(s)	96, 216, 236, 1203
BlsI	GCNGC	4 cut(s)	97, 217, 237, 1204
Bme1390I	CCNGG	4 cut(s)	173, 284, 588, 1186
Bme18I	GGWCC	1 cut(s)	908
BmgT120I	GGNCC	1 cut(s)	908
BmrFI	CCNGG	4 cut(s)	173, 284, 588, 1186
BmsI	GCATC	1 cut(s)	451
BpiI	GAAGAC	1 cut(s)	129
BpmI	CTGGAG	3 cut(s)	11, 155, 609
Bpu10I	CCTNAGC	1 cut(s)	647
Bpu14I	TTCGAA	1 cut(s)	659
BpuEI	CTTGAG	4 cut(s)	131, 257, 553, 575
BpuMI	CCSGG	2 cut(s)	284, 1186
BsaBI	GATNNNNATC	1 cut(s)	719
BsaHI	GRCGYC	3 cut(s)	213, 228, 261
BsaJI	CCNNGG	6 cut(s)	153, 198, 306, 731, 807, 1080
BsaWI	WCCGGW	3 cut(s)	445, 1209, 1221
BsaXI	ACNNNNNCTCC	6 cut(s)	9, 39, 122, 152, 939, 969
Bsc4I	CCNNNNNNNGG	3 cut(s)	222, 289, 700
Bse118I	RCCGGY	2 cut(s)	919, 1209
Bse1I	ACTGG	1 cut(s)	863
Bse21I	CCTNAGG	1 cut(s)	852
Bse3DI	GCAATG	1 cut(s)	192
Bse8I	GATNNNNATC	1 cut(s)	719
BseAI	TCCGGA	1 cut(s)	1221
BseBI	CCWGG	2 cut(s)	173, 588
BseDI	CCNNGG	6 cut(s)	153, 198, 306, 731, 807, 1080
BseGI	GGATG	3 cut(s)	264, 442, 862
BseJI	GATNNNNATC	1 cut(s)	719
BseLI	CCNNNNNNNGG	3 cut(s)	222, 289, 700
BseMI	GCAATG	1 cut(s)	192
BseMII	CTCAG	4 cut(s)	843, 872, 1032, 1179
BseNI	ACTGG	1 cut(s)	863
BseRI	GAGGAG	4 cut(s)	260, 389, 527, 960
BseXI	GCAGC	2 cut(s)	222, 1189
BshFI	GGCC	5 cut(s)	44, 257, 707, 850, 1148
BshTI	ACCGGT	1 cut(s)	1209
BsiHKAI	GWGCWC	1 cut(s)	180
BsiSI	CCGG	8 cut(s)	243, 283, 446, 920, 1149, 1186, 1210, 1222
BslFI	GGGAC	4 cut(s)	305, 521, 1016, 1037
BslI	CCNNNNNNNGG	3 cut(s)	222, 289, 700
BsmAI	GTCTC	4 cut(s)	79, 142, 240, 821
BsmBI	CGTCTC	2 cut(s)	79, 240
BsmFI	GGGAC	4 cut(s)	305, 521, 1016, 1037
BsnI	GGCC	5 cut(s)	44, 257, 707, 850, 1148
Bsp119I	TTCGAA	1 cut(s)	659
Bsp1286I	GDGCHC	1 cut(s)	180
Bsp13I	TCCGGA	1 cut(s)	1221
Bsp143I	GATC	4 cut(s)	594, 619, 666, 966
BspACI	CCGC	3 cut(s)	96, 216, 1123
BspANI	GGCC	5 cut(s)	44, 257, 707, 850, 1148
BspCNI	CTCAG	4 cut(s)	844, 873, 1033, 1178
BspEI	TCCGGA	1 cut(s)	1221
BspPI	GGATC	2 cut(s)	602, 661
BspT104I	TTCGAA	1 cut(s)	659
BspTI	CTTAAG	2 cut(s)	521, 623
BsrBI	CCGCTC	1 cut(s)	98
BsrDI	GCAATG	1 cut(s)	192
BsrFI	RCCGGY	2 cut(s)	919, 1209
BsrI	ACTGG	1 cut(s)	863
BssAI	RCCGGY	2 cut(s)	919, 1209
BssECI	CCNNGG	6 cut(s)	153, 198, 306, 731, 807, 1080
BssMI	GATC	4 cut(s)	594, 619, 666, 966
BssNI	GRCGYC	3 cut(s)	213, 228, 261
BssT1I	CCWWGG	1 cut(s)	807
Bst2UI	CCWGG	2 cut(s)	173, 588
Bst4CI	ACNGT	4 cut(s)	732, 908, 937, 1037
Bst6I	CTCTTC	1 cut(s)	82
BstACI	GRCGYC	3 cut(s)	213, 228, 261
BstAFI	CTTAAG	2 cut(s)	521, 623
BstBI	TTCGAA	1 cut(s)	659
BstC8I	GCNNGC	2 cut(s)	208, 240
BstDEI	CTNAG	6 cut(s)	647, 786, 852, 881, 1041, 1165
BstDSI	CCRYGG	2 cut(s)	731, 1080
BstF5I	GGATG	3 cut(s)	264, 442, 862
BstH2I	RGCGCY	1 cut(s)	1121
BstHHI	GCGC	1 cut(s)	1120
BstKTI	GATC	4 cut(s)	597, 622, 669, 969
BstMAI	GTCTC	4 cut(s)	79, 142, 240, 821
BstMBI	GATC	4 cut(s)	594, 619, 666, 966
BstMWI	GCNNNNNNNGC	3 cut(s)	203, 212, 583
BstNI	CCWGG	2 cut(s)	173, 588
BstNSI	RCATGY	3 cut(s)	322, 490, 634
BstSCI	CCNGG	4 cut(s)	171, 282, 586, 1184
BstV1I	GCAGC	2 cut(s)	222, 1189
BstV2I	GAAGAC	1 cut(s)	129
BstX2I	RGATCY	1 cut(s)	666
BstYI	RGATCY	1 cut(s)	666
Bsu36I	CCTNAGG	1 cut(s)	852
BsuRI	GGCC	5 cut(s)	44, 257, 707, 850, 1148
BtgI	CCRYGG	2 cut(s)	731, 1080
BtsCI	GGATG	3 cut(s)	264, 442, 862
BtsIMutI	CAGTG	5 cut(s)	615, 748, 933, 1042, 1173
Cac8I	GCNNGC	2 cut(s)	208, 240
CfoI	GCGC	1 cut(s)	1120
Cfr10I	RCCGGY	2 cut(s)	919, 1209
Cfr13I	GGNCC	1 cut(s)	908
CseI	GACGC	2 cut(s)	217, 221
Csp6I	GTAC	5 cut(s)	728, 736, 1033, 1207, 1213
CspAI	ACCGGT	1 cut(s)	1209
CviAII	CATG	6 cut(s)	220, 319, 422, 487, 631, 999
CviQI	GTAC	5 cut(s)	728, 736, 1033, 1207, 1213
DdeI	CTNAG	6 cut(s)	647, 786, 852, 881, 1041, 1165
DpnI	GATC	4 cut(s)	596, 621, 668, 968
DpnII	GATC	4 cut(s)	594, 619, 666, 966
EaeI	YGGCCR	1 cut(s)	705
Eam1104I	CTCTTC	1 cut(s)	82
EarI	CTCTTC	1 cut(s)	82
Ecl136II	GAGCTC	1 cut(s)	178
Eco130I	CCWWGG	1 cut(s)	807
Eco147I	AGGCCT	1 cut(s)	850
Eco24I	GRGCYC	1 cut(s)	180
Eco32I	GATATC	1 cut(s)	804
Eco47I	GGWCC	1 cut(s)	908
Eco47III	AGCGCT	1 cut(s)	1119
Eco53kI	GAGCTC	1 cut(s)	178
Eco57I	CTGAAG	2 cut(s)	724, 1131
Eco81I	CCTNAGG	1 cut(s)	852
EcoICRI	GAGCTC	1 cut(s)	178
EcoRI	GAATTC	1 cut(s)	929
EcoRII	CCWGG	2 cut(s)	171, 586
EcoRV	GATATC	1 cut(s)	804
EcoT14I	CCWWGG	1 cut(s)	807
EcoT22I	ATGCAT	1 cut(s)	423
EcoT38I	GRGCYC	1 cut(s)	180
ErhI	CCWWGG	1 cut(s)	807
Esp3I	CGTCTC	2 cut(s)	79, 240
FaeI	CATG	6 cut(s)	223, 322, 425, 490, 634, 1002
FalI	AAGNNNNNCTT	6 cut(s)	53, 85, 515, 547, 640, 672
FaqI	GGGAC	4 cut(s)	305, 521, 1016, 1037
FatI	CATG	6 cut(s)	219, 318, 421, 486, 630, 998
FblI	GTMKAC	1 cut(s)	886
Fnu4HI	GCNGC	4 cut(s)	96, 216, 236, 1203
FokI	GGATG	3 cut(s)	251, 429, 869
FriOI	GRGCYC	1 cut(s)	180
Fsp4HI	GCNGC	4 cut(s)	96, 216, 236, 1203
FspBI	CTAG	2 cut(s)	323, 1240
GlaI	GCGC	1 cut(s)	1119
GluI	GCNGC	4 cut(s)	96, 216, 236, 1203
GsuI	CTGGAG	3 cut(s)	11, 155, 609
HaeII	RGCGCY	1 cut(s)	1121
HaeIII	GGCC	5 cut(s)	44, 257, 707, 850, 1148
HapII	CCGG	8 cut(s)	243, 283, 446, 920, 1149, 1186, 1210, 1222
HgaI	GACGC	2 cut(s)	217, 221
HhaI	GCGC	1 cut(s)	1120
Hin1I	GRCGYC	3 cut(s)	213, 228, 261
Hin1II	CATG	6 cut(s)	223, 322, 425, 490, 634, 1002
Hin6I	GCGC	1 cut(s)	1118
HinP1I	GCGC	1 cut(s)	1118
HincII	GTYRAC	2 cut(s)	887, 958
HindII	GTYRAC	2 cut(s)	887, 958
HindIII	AAGCTT	2 cut(s)	67, 518
HinfI	GANTC	3 cut(s)	5, 661, 883
HpaI	GTTAAC	1 cut(s)	958
HpaII	CCGG	8 cut(s)	243, 283, 446, 920, 1149, 1186, 1210, 1222
Hpy166II	GTNNAC	5 cut(s)	878, 887, 958, 1058, 1171
Hpy188I	TCNGA	8 cut(s)	104, 157, 202, 666, 774, 928, 966, 1111
Hpy188III	TCNNGA	5 cut(s)	335, 532, 554, 683, 1222
Hpy8I	GTNNAC	5 cut(s)	878, 887, 958, 1058, 1171
Hpy99I	CGWCG	3 cut(s)	95, 215, 263
HpyAV	CCTTC	6 cut(s)	157, 340, 395, 553, 806, 1189
HpyCH4III	ACNGT	4 cut(s)	732, 908, 937, 1037
HpyCH4IV	ACGT	2 cut(s)	90, 261
HpyCH4V	TGCA	5 cut(s)	421, 577, 824, 901, 989
HpyF10VI	GCNNNNNNNGC	3 cut(s)	203, 212, 583
HpyF3I	CTNAG	6 cut(s)	647, 786, 852, 881, 1041, 1165
HpySE526I	ACGT	2 cut(s)	90, 261
Hsp92I	GRCGYC	3 cut(s)	213, 228, 261
Hsp92II	CATG	6 cut(s)	223, 322, 425, 490, 634, 1002
HspAI	GCGC	1 cut(s)	1118
Kpn2I	TCCGGA	1 cut(s)	1221
KspAI	GTTAAC	1 cut(s)	958
Kzo9I	GATC	4 cut(s)	594, 619, 666, 966
LmnI	GCTCC	3 cut(s)	175, 394, 1125
Lsp1109I	GCAGC	2 cut(s)	222, 1189
LweI	GCATC	1 cut(s)	451
MaeI	CTAG	2 cut(s)	323, 1240
MaeII	ACGT	2 cut(s)	90, 261
MaeIII	GTNAC	1 cut(s)	613
MalI	GATC	4 cut(s)	596, 621, 668, 968
MbiI	CCGCTC	1 cut(s)	98
MboI	GATC	4 cut(s)	594, 619, 666, 966
MboII	GAAGA	4 cut(s)	69, 134, 172, 1124
MflI	RGATCY	1 cut(s)	666
MhlI	GDGCHC	1 cut(s)	180
MlsI	TGGCCA	1 cut(s)	707
MluCI	AATT	5 cut(s)	468, 839, 929, 993, 1174
MluNI	TGGCCA	1 cut(s)	707
MlyI	GAGTC	1 cut(s)	892
MmeI	TCCRAC	1 cut(s)	994
Mox20I	TGGCCA	1 cut(s)	707
Mph1103I	ATGCAT	1 cut(s)	423
MroI	TCCGGA	1 cut(s)	1221
MroXI	GAANNNNTTC	2 cut(s)	68, 109
MscI	TGGCCA	1 cut(s)	707
MseI	TTAA	6 cut(s)	390, 474, 522, 582, 624, 957
Msp20I	TGGCCA	1 cut(s)	707
MspCI	CTTAAG	2 cut(s)	521, 623
MspI	CCGG	8 cut(s)	243, 283, 446, 920, 1149, 1186, 1210, 1222
MspR9I	CCNGG	4 cut(s)	173, 284, 588, 1186
MvaI	CCWGG	2 cut(s)	173, 588
MwoI	GCNNNNNNNGC	3 cut(s)	203, 212, 583
NciI	CCSGG	2 cut(s)	284, 1186
NdeII	GATC	4 cut(s)	594, 619, 666, 966
NlaIII	CATG	6 cut(s)	223, 322, 425, 490, 634, 1002
NmeAIII	GCCGAG	2 cut(s)	331, 1116
NmuCI	GTSAC	1 cut(s)	613
NsiI	ATGCAT	1 cut(s)	423
NspI	RCATGY	3 cut(s)	322, 490, 634
NspV	TTCGAA	1 cut(s)	659
PceI	AGGCCT	1 cut(s)	850
PciI	ACATGT	1 cut(s)	486
PcsI	WCGNNNNNNNCGW	2 cut(s)	246, 255
PdmI	GAANNNNTTC	2 cut(s)	68, 109
PfeI	GAWTC	1 cut(s)	661
PflFI	GACNNNGTC	1 cut(s)	882
PfoI	TCCNGGA	2 cut(s)	171, 1184
PinAI	ACCGGT	1 cut(s)	1209
PkrI	GCNGC	4 cut(s)	97, 217, 237, 1204
PleI	GAGTC	1 cut(s)	891
PpsI	GAGTC	1 cut(s)	891
PscI	ACATGT	1 cut(s)	486
PsiI	TTATAA	1 cut(s)	456
Psp124BI	GAGCTC	1 cut(s)	180
Psp6I	CCWGG	2 cut(s)	171, 586
PspGI	CCWGG	2 cut(s)	171, 586
PspPI	GGNCC	1 cut(s)	908
PsrI	GAACNNNNNNTAC	2 cut(s)	743, 775
PsuI	RGATCY	1 cut(s)	666
PsyI	GACNNNGTC	1 cut(s)	882
RsaI	GTAC	5 cut(s)	729, 737, 1034, 1208, 1214
RsaNI	GTAC	5 cut(s)	728, 736, 1033, 1207, 1213
SacI	GAGCTC	1 cut(s)	180
SalI	GTCGAC	1 cut(s)	885
SaqAI	TTAA	6 cut(s)	390, 474, 522, 582, 624, 957
SatI	GCNGC	4 cut(s)	96, 216, 236, 1203
Sau3AI	GATC	4 cut(s)	594, 619, 666, 966
Sau96I	GGNCC	1 cut(s)	908
SchI	GAGTC	1 cut(s)	892
ScrFI	CCNGG	4 cut(s)	173, 284, 588, 1186
SduI	GDGCHC	1 cut(s)	180
SfaNI	GCATC	1 cut(s)	451
SfuI	TTCGAA	1 cut(s)	659
SinI	GGWCC	1 cut(s)	908
SmlI	CTYRAG	6 cut(s)	146, 272, 521, 532, 554, 623
SmoI	CTYRAG	6 cut(s)	146, 272, 521, 532, 554, 623
Sse9I	AATT	5 cut(s)	468, 839, 929, 993, 1174
SseBI	AGGCCT	1 cut(s)	850
SsiI	CCGC	3 cut(s)	96, 216, 1123
SspMI	CTAG	2 cut(s)	323, 1240
SstI	GAGCTC	1 cut(s)	180
StuI	AGGCCT	1 cut(s)	850
StyD4I	CCNGG	4 cut(s)	171, 282, 586, 1184
StyI	CCWWGG	1 cut(s)	807
TaaI	ACNGT	4 cut(s)	732, 908, 937, 1037
TaiI	ACGT	2 cut(s)	93, 264
TaqI	TCGA	4 cut(s)	659, 755, 886, 969
TasI	AATT	5 cut(s)	468, 839, 929, 993, 1174
TatI	WGTACW	1 cut(s)	1032
TauI	GCSGC	2 cut(s)	98, 218
TfiI	GAWTC	1 cut(s)	661
Tru1I	TTAA	6 cut(s)	390, 474, 522, 582, 624, 957
Tru9I	TTAA	6 cut(s)	390, 474, 522, 582, 624, 957
TscAI	CASTG	5 cut(s)	622, 748, 940, 1042, 1173
TseFI	GTSAC	1 cut(s)	613
TseI	GCWGC	2 cut(s)	235, 1202
Tsp45I	GTSAC	1 cut(s)	613
TspDTI	ATGAA	3 cut(s)	101, 507, 709
TspGWI	ACGGA	2 cut(s)	287, 756
TspRI	CASTG	5 cut(s)	622, 748, 940, 1042, 1173
Tth111I	GACNNNGTC	1 cut(s)	882
Vha464I	CTTAAG	2 cut(s)	521, 623
VpaK11BI	GGWCC	1 cut(s)	908
XapI	RAATTY	3 cut(s)	468, 839, 929
XceI	RCATGY	3 cut(s)	322, 490, 634
XmiI	GTMKAC	1 cut(s)	886
XmnI	GAANNNNTTC	2 cut(s)	68, 109
XspI	CTAG	2 cut(s)	323, 1240
ZraI	GACGTC	1 cut(s)	262
Zsp2I	ATGCAT	1 cut(s)	423

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.