Rorug06G0023600

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010322
Physical Location & Seq
Forward (+)
2905266 .. 2905940
675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0023600.1

Sequence Viewer

Length: 426 bp
ATGGAGTGGTTCTACCCTAAGAGAAGGGGTCCAGAGTGGAAGCAAGGGTGGACTGGCCAGACCCTCACCTCTATTTCTGCACCACCTTTACATTTACTCACCATCTTTGCCATTGTAATTTTATTACTATGGCTTTCTCAATACACCGAGTACAAATCCCAAATGCAACAGACCGCCGTTAACTTCCAGATATTGTTAATGTTGCTGCCTGTTTTCTTAATATTTTTGGTAGCTTCATGTTCTTCGATTTCCTCCGGTGGATGGTTCAACTTCAGGCGCCGGCATCCACAGCACGAGTTGGTCAACCAAGCTGGTGGTGGCTCGCCGTGGGGTGTTGCCATATTGGTAGCGCTGGTTTTGGTCTTGCTATCATACCAATCATCTTTTCATTCGAAGTGGTTTGGACCTCTTGGGAGATTAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

141

Amino Acids

16.06

Weight (kDa)

9.8

Isoelectric Point (pI)

57.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 276
AciI CCGC 1 cut(s) 174
AcoI YGGCCR 1 cut(s) 55
AcuI CTGAAG 1 cut(s) 256
AcyI GRCGYC 1 cut(s) 277
AfaI GTAC 1 cut(s) 152
AfeI AGCGCT 1 cut(s) 351
AfiI CCNNNNNNNGG 1 cut(s) 261
AgsI TTSAA 1 cut(s) 268
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
AluBI AGCT 2 cut(s) 233, 311
AluI AGCT 2 cut(s) 233, 311
Aor51HI AGCGCT 1 cut(s) 351
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 205
AspLEI GCGC 2 cut(s) 279, 352
AspS9I GGNCC 2 cut(s) 29, 404
AsuHPI GGTGA 2 cut(s) 58, 91
AsuII TTCGAA 1 cut(s) 392
AvaII GGWCC 2 cut(s) 29, 404
BalI TGGCCA 1 cut(s) 57
BanI GGYRCC 1 cut(s) 276
BauI CACGAG 1 cut(s) 293
BbvI GCAGC 1 cut(s) 192
BccI CCATC 2 cut(s) 110, 255
BceAI ACGGC 2 cut(s) 161, 310
BfoI RGCGCY 2 cut(s) 280, 353
BisI GCNGC 1 cut(s) 206
BlsI GCNGC 1 cut(s) 207
Bme18I GGWCC 2 cut(s) 29, 404
BmgT120I GGNCC 2 cut(s) 29, 404
BmiI GGNNCC 2 cut(s) 30, 278
BmsI GCATC 1 cut(s) 292
Bpu14I TTCGAA 1 cut(s) 392
BsaHI GRCGYC 1 cut(s) 277
BsaJI CCNNGG 1 cut(s) 326
BsaWI WCCGGW 1 cut(s) 254
Bsc4I CCNNNNNNNGG 1 cut(s) 261
Bse118I RCCGGY 1 cut(s) 279
Bse1I ACTGG 1 cut(s) 58
BseDI CCNNGG 1 cut(s) 326
BseGI GGATG 2 cut(s) 266, 283
BseLI CCNNNNNNNGG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 58
BseXI GCAGC 1 cut(s) 192
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 1 cut(s) 57
BshNI GGYRCC 1 cut(s) 276
BsiSI CCGG 2 cut(s) 255, 280
BslI CCNNNNNNNGG 1 cut(s) 261
BsnI GGCC 1 cut(s) 57
Bsp119I TTCGAA 1 cut(s) 392
BspACI CCGC 1 cut(s) 174
BspANI GGCC 1 cut(s) 57
BspLI GGNNCC 2 cut(s) 30, 278
BspT104I TTCGAA 1 cut(s) 392
BspT107I GGYRCC 1 cut(s) 276
BsrFI RCCGGY 1 cut(s) 279
BsrI ACTGG 1 cut(s) 58
BssAI RCCGGY 1 cut(s) 279
BssECI CCNNGG 1 cut(s) 326
BssNI GRCGYC 1 cut(s) 277
BssSI CACGAG 1 cut(s) 293
Bst2BI CACGAG 1 cut(s) 293
BstACI GRCGYC 1 cut(s) 277
BstBI TTCGAA 1 cut(s) 392
BstC8I GCNNGC 2 cut(s) 281, 323
BstDEI CTNAG 1 cut(s) 18
BstDSI CCRYGG 1 cut(s) 326
BstF5I GGATG 2 cut(s) 266, 283
BstH2I RGCGCY 2 cut(s) 280, 353
BstHHI GCGC 2 cut(s) 279, 352
BstMWI GCNNNNNNNGC 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 192
BstXI CCANNNNNNTGG 1 cut(s) 314
BsuRI GGCC 1 cut(s) 57
BtgI CCRYGG 1 cut(s) 326
BtsCI GGATG 2 cut(s) 266, 283
Cac8I GCNNGC 2 cut(s) 281, 323
CfoI GCGC 2 cut(s) 279, 352
Cfr10I RCCGGY 1 cut(s) 279
Cfr13I GGNCC 2 cut(s) 29, 404
Csp6I GTAC 1 cut(s) 151
CviAII CATG 1 cut(s) 237
CviJI RGCY 5 cut(s) 57, 133, 233, 311, 321
CviKI_1 RGCY 5 cut(s) 57, 133, 233, 311, 321
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 1 cut(s) 18
DinI GGCGCC 1 cut(s) 278
EaeI YGGCCR 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 29, 404
Eco47III AGCGCT 1 cut(s) 351
Eco57I CTGAAG 1 cut(s) 256
EgeI GGCGCC 1 cut(s) 278
EheI GGCGCC 1 cut(s) 278
FaeI CATG 1 cut(s) 240
FaiI YATR 4 cut(s) 130, 238, 341, 373
FatI CATG 1 cut(s) 236
Fnu4HI GCNGC 1 cut(s) 206
FokI GGATG 2 cut(s) 270, 273
Fsp4HI GCNGC 1 cut(s) 206
GlaI GCGC 2 cut(s) 278, 351
GluI GCNGC 1 cut(s) 206
HaeII RGCGCY 2 cut(s) 280, 353
HaeIII GGCC 1 cut(s) 57
HapII CCGG 2 cut(s) 255, 280
HhaI GCGC 2 cut(s) 279, 352
Hin1I GRCGYC 1 cut(s) 277
Hin1II CATG 1 cut(s) 240
Hin6I GCGC 2 cut(s) 277, 350
HinP1I GCGC 2 cut(s) 277, 350
HincII GTYRAC 2 cut(s) 181, 304
HindII GTYRAC 2 cut(s) 181, 304
HpaI GTTAAC 1 cut(s) 181
HpaII CCGG 2 cut(s) 255, 280
HphI GGTGA 2 cut(s) 58, 91
Hpy166II GTNNAC 3 cut(s) 51, 181, 304
Hpy188III TCNNGA 2 cut(s) 32, 187
Hpy8I GTNNAC 3 cut(s) 51, 181, 304
HpyAV CCTTC 1 cut(s) 18
HpyCH4V TGCA 2 cut(s) 80, 166
HpyF10VI GCNNNNNNNGC 1 cut(s) 289
HpyF3I CTNAG 1 cut(s) 18
Hsp92I GRCGYC 1 cut(s) 277
Hsp92II CATG 1 cut(s) 240
HspAI GCGC 2 cut(s) 277, 350
KasI GGCGCC 1 cut(s) 276
KroI GCCGGC 1 cut(s) 279
KroNI GCCGGC 1 cut(s) 281
KspAI GTTAAC 1 cut(s) 181
Lsp1109I GCAGC 1 cut(s) 192
LweI GCATC 1 cut(s) 292
MboII GAAGA 1 cut(s) 234
MlsI TGGCCA 1 cut(s) 57
MluCI AATT 1 cut(s) 117
MluNI TGGCCA 1 cut(s) 57
Mly113I GGCGCC 1 cut(s) 277
MnlI CCTC 4 cut(s) 74, 79, 262, 417
Mox20I TGGCCA 1 cut(s) 57
MroNI GCCGGC 1 cut(s) 279
MscI TGGCCA 1 cut(s) 57
MseI TTAA 3 cut(s) 180, 197, 218
Msp20I TGGCCA 1 cut(s) 57
MspI CCGG 2 cut(s) 255, 280
MwoI GCNNNNNNNGC 1 cut(s) 289
NaeI GCCGGC 1 cut(s) 281
NarI GGCGCC 1 cut(s) 277
NgoMIV GCCGGC 1 cut(s) 279
NlaIII CATG 1 cut(s) 240
NlaIV GGNNCC 2 cut(s) 30, 278
NspV TTCGAA 1 cut(s) 392
PdiI GCCGGC 1 cut(s) 281
PkrI GCNGC 1 cut(s) 207
PluTI GGCGCC 1 cut(s) 280
PspN4I GGNNCC 2 cut(s) 30, 278
PspPI GGNCC 2 cut(s) 29, 404
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
SaqAI TTAA 3 cut(s) 180, 197, 218
SatI GCNGC 1 cut(s) 206
Sau96I GGNCC 2 cut(s) 29, 404
SetI ASST 5 cut(s) 71, 88, 235, 313, 409
SfaNI GCATC 1 cut(s) 292
SfoI GGCGCC 1 cut(s) 278
SfuI TTCGAA 1 cut(s) 392
SinI GGWCC 2 cut(s) 29, 404
Sse9I AATT 1 cut(s) 117
SsiI CCGC 1 cut(s) 174
SspDI GGCGCC 1 cut(s) 276
SspI AATATT 1 cut(s) 222
TaqI TCGA 2 cut(s) 245, 392
TasI AATT 1 cut(s) 117
TatI WGTACW 1 cut(s) 150
Tru1I TTAA 3 cut(s) 180, 197, 218
Tru9I TTAA 3 cut(s) 180, 197, 218
TseI GCWGC 1 cut(s) 205
TspDTI ATGAA 2 cut(s) 225, 377
VpaK11BI GGWCC 2 cut(s) 29, 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.