RLG00000014119

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010322
Physical Location & Seq
Reverse (-)
47617292 .. 47625435
8144 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014119

Sequence Viewer

Length: 621 bp
ATGGCATCACCACCGTCAATTGCCAGCCCTCTAGCCCCACCGGCGGCATGCTCGTCTTCGTTAGTGGTAATTTCCAACTCGCCGGTGAACAGCACGCCCTCTCTAAGAAAGCCCTCTCACCTCCCCAAAAACCTCCAAGAAAAGCTTCATCATCTCTCTAATGGTGTCAAACTGCTTGCCAAAGACGACAAAGATGGCGTCATCCTCCTCAAGTTTCTCCGAGCCTTGGATTTCCAGGTTGCTGACGCTTTCAACATGATAGTCAAGTGCCTGGAGTGGAGGAAGGAGTTCAAAGCAAACGAGGTTGCTGAGGAGGACTTGGGATTTAAGGAGCTCGAAGGTGTTGTTTCTTACATGCACGGATTTGATAGGTGCAGACACCCTATTTGTTATAATGCTTCTGGAGTGTTCAAGGACAAAGACATGTATGAGAGGATTTTCGGGGACGAGGAGAAGCTCAAGAAGTTCTTGAGATGGAGAGTTCAGGTCCTTAAAAAGGGTATCAATGTCTTGCATTTTAAGCCAGGAGGGATCAACTCCATCATCCAAGTCACTGATCTCAAGGACATGCCCAAAAAAGAGCTCAGGGTTGCTTCAAATCAGATCATTTTGCTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

207

Amino Acids

23.24

Weight (kDa)

9.21

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 59 - 89 8e-07 CRAL/TRIO, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 393
AciI CCGC 1 cut(s) 44
AclWI GGATC 1 cut(s) 539
AcyI GRCGYC 1 cut(s) 198
AfiI CCNNNNNNNGG 3 cut(s) 43, 226, 496
AflIII ACRYGT 1 cut(s) 423
AgsI TTSAA 4 cut(s) 253, 292, 412, 597
AjnI CCWGG 3 cut(s) 234, 270, 523
AjuI GAANNNNNNNTTGG 2 cut(s) 129, 161
AluBI AGCT 4 cut(s) 145, 334, 457, 583
AluI AGCT 4 cut(s) 145, 334, 457, 583
Alw21I GWGCWC 2 cut(s) 336, 585
AlwI GGATC 1 cut(s) 539
ArsI GACNNNNNNTTYG 4 cut(s) 183, 215, 369, 401
Asp700I GAANNNNTTC 2 cut(s) 144, 287
AspS9I GGNCC 1 cut(s) 487
AsuHPI GGTGA 2 cut(s) 97, 110
AvaII GGWCC 1 cut(s) 487
BanII GRGCYC 2 cut(s) 336, 585
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 2 cut(s) 336, 585
BbvCI CCTCAGC 1 cut(s) 309
BccI CCATC 3 cut(s) 188, 468, 548
BciT130I CCWGG 3 cut(s) 236, 272, 525
BfaI CTAG 1 cut(s) 32
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 1 cut(s) 45
BlsI GCNGC 1 cut(s) 46
Bme1390I CCNGG 3 cut(s) 236, 272, 525
Bme18I GGWCC 1 cut(s) 487
BmgT120I GGNCC 1 cut(s) 487
BmrFI CCNGG 3 cut(s) 236, 272, 525
BmsI GCATC 1 cut(s) 14
BpiI GAAGAC 1 cut(s) 48
BpmI CTGGAG 2 cut(s) 293, 423
Bpu10I CCTNAGC 2 cut(s) 309, 584
BpuEI CTTGAG 4 cut(s) 194, 443, 490, 545
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 1 cut(s) 225
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 226, 496
Bse118I RCCGGY 2 cut(s) 40, 82
BseBI CCWGG 3 cut(s) 236, 272, 525
BseDI CCNNGG 1 cut(s) 225
BseGI GGATG 2 cut(s) 201, 543
BseLI CCNNNNNNNGG 3 cut(s) 43, 226, 496
BseMII CTCAG 2 cut(s) 300, 598
BseRI GAGGAG 3 cut(s) 197, 326, 464
BsgI GTGCAG 1 cut(s) 394
BsiHKAI GWGCWC 2 cut(s) 336, 585
BsiSI CCGG 2 cut(s) 41, 83
BslFI GGGAC 1 cut(s) 458
BslI CCNNNNNNNGG 3 cut(s) 43, 226, 496
BsmFI GGGAC 1 cut(s) 458
Bsp1286I GDGCHC 2 cut(s) 336, 585
Bsp143I GATC 3 cut(s) 531, 556, 603
BspACI CCGC 1 cut(s) 44
BspCNI CTCAG 2 cut(s) 301, 597
BspPI GGATC 1 cut(s) 539
BsrFI RCCGGY 2 cut(s) 40, 82
BssAI RCCGGY 2 cut(s) 40, 82
BssECI CCNNGG 1 cut(s) 225
BssMI GATC 3 cut(s) 531, 556, 603
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 225
Bst2UI CCWGG 3 cut(s) 236, 272, 525
Bst4CI ACNGT 1 cut(s) 15
BstACI GRCGYC 1 cut(s) 198
BstC8I GCNNGC 4 cut(s) 25, 49, 95, 177
BstDEI CTNAG 3 cut(s) 104, 309, 584
BstENI CCTNNNNNAGG 1 cut(s) 494
BstF5I GGATG 2 cut(s) 201, 543
BstKTI GATC 3 cut(s) 534, 559, 606
BstMBI GATC 3 cut(s) 531, 556, 603
BstMWI GCNNNNNNNGC 2 cut(s) 41, 520
BstNI CCWGG 3 cut(s) 236, 272, 525
BstNSI RCATGY 4 cut(s) 51, 358, 427, 571
BstSCI CCNGG 3 cut(s) 234, 270, 523
BstV2I GAAGAC 1 cut(s) 48
BtsCI GGATG 2 cut(s) 201, 543
BtsIMutI CAGTG 1 cut(s) 552
Cac8I GCNNGC 4 cut(s) 25, 49, 95, 177
Cfr10I RCCGGY 2 cut(s) 40, 82
Cfr13I GGNCC 1 cut(s) 487
CseI GACGC 2 cut(s) 187, 254
CviAII CATG 5 cut(s) 48, 256, 355, 424, 568
CviJI RGCY 9 cut(s) 27, 35, 112, 145, 224, 334, 457, 523, 583
CviKI_1 RGCY 9 cut(s) 27, 35, 112, 145, 224, 334, 457, 523, 583
DdeI CTNAG 3 cut(s) 104, 309, 584
DpnI GATC 3 cut(s) 533, 558, 605
DpnII GATC 3 cut(s) 531, 556, 603
Ecl136II GAGCTC 2 cut(s) 334, 583
Eco130I CCWWGG 1 cut(s) 225
Eco24I GRGCYC 2 cut(s) 336, 585
Eco47I GGWCC 1 cut(s) 487
Eco53kI GAGCTC 2 cut(s) 334, 583
EcoICRI GAGCTC 2 cut(s) 334, 583
EcoNI CCTNNNNNAGG 1 cut(s) 494
EcoO109I RGGNCCY 1 cut(s) 487
EcoRII CCWGG 3 cut(s) 234, 270, 523
EcoT14I CCWWGG 1 cut(s) 225
EcoT38I GRGCYC 2 cut(s) 336, 585
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 5 cut(s) 51, 259, 358, 427, 571
FaiI YATR 7 cut(s) 49, 257, 356, 393, 425, 429, 569
FalI AAGNNNNNCTT 4 cut(s) 129, 161, 452, 484
FaqI GGGAC 1 cut(s) 458
FatI CATG 5 cut(s) 47, 255, 354, 423, 567
Fnu4HI GCNGC 1 cut(s) 45
FokI GGATG 2 cut(s) 188, 530
FriOI GRGCYC 2 cut(s) 336, 585
Fsp4HI GCNGC 1 cut(s) 45
FspBI CTAG 1 cut(s) 32
GluI GCNGC 1 cut(s) 45
GsuI CTGGAG 2 cut(s) 293, 423
HapII CCGG 2 cut(s) 41, 83
HgaI GACGC 2 cut(s) 187, 254
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 5 cut(s) 51, 259, 358, 427, 571
HindIII AAGCTT 1 cut(s) 143
HpaII CCGG 2 cut(s) 41, 83
HphI GGTGA 2 cut(s) 97, 110
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 2 cut(s) 221, 603
Hpy188III TCNNGA 3 cut(s) 402, 460, 469
Hpy8I GTNNAC 1 cut(s) 88
HpyAV CCTTC 2 cut(s) 277, 332
HpyCH4III ACNGT 1 cut(s) 15
HpyCH4V TGCA 3 cut(s) 358, 375, 514
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 520
HpyF3I CTNAG 3 cut(s) 104, 309, 584
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 5 cut(s) 51, 259, 358, 427, 571
Kzo9I GATC 3 cut(s) 531, 556, 603
LmnI GCTCC 1 cut(s) 331
LweI GCATC 1 cut(s) 14
MaeI CTAG 1 cut(s) 32
MaeIII GTNAC 1 cut(s) 550
MalI GATC 3 cut(s) 533, 558, 605
MboI GATC 3 cut(s) 531, 556, 603
MboII GAAGA 1 cut(s) 48
MfeI CAATTG 1 cut(s) 18
MhlI GDGCHC 2 cut(s) 336, 585
MluCI AATT 2 cut(s) 18, 69
MmeI TCCRAC 1 cut(s) 99
MroXI GAANNNNTTC 2 cut(s) 144, 287
MseI TTAA 3 cut(s) 327, 492, 519
MspI CCGG 2 cut(s) 41, 83
MspR9I CCNGG 3 cut(s) 236, 272, 525
MunI CAATTG 1 cut(s) 18
MvaI CCWGG 3 cut(s) 236, 272, 525
MwoI GCNNNNNNNGC 2 cut(s) 41, 520
NdeII GATC 3 cut(s) 531, 556, 603
NlaIII CATG 5 cut(s) 51, 259, 358, 427, 571
NmuCI GTSAC 1 cut(s) 550
NspI RCATGY 4 cut(s) 51, 358, 427, 571
PaeI GCATGC 1 cut(s) 51
PciI ACATGT 1 cut(s) 423
PdmI GAANNNNTTC 2 cut(s) 144, 287
PkrI GCNGC 1 cut(s) 46
PpuMI RGGWCCY 1 cut(s) 487
PscI ACATGT 1 cut(s) 423
PsiI TTATAA 1 cut(s) 393
Psp124BI GAGCTC 2 cut(s) 336, 585
Psp5II RGGWCCY 1 cut(s) 487
Psp6I CCWGG 3 cut(s) 234, 270, 523
PspGI CCWGG 3 cut(s) 234, 270, 523
PspPI GGNCC 1 cut(s) 487
PspPPI RGGWCCY 1 cut(s) 487
SacI GAGCTC 2 cut(s) 336, 585
SaqAI TTAA 3 cut(s) 327, 492, 519
SatI GCNGC 1 cut(s) 45
Sau3AI GATC 3 cut(s) 531, 556, 603
Sau96I GGNCC 1 cut(s) 487
ScrFI CCNGG 3 cut(s) 236, 272, 525
SduI GDGCHC 2 cut(s) 336, 585
SfaNI GCATC 1 cut(s) 14
SgrAI CRCCGGYG 2 cut(s) 40, 82
SinI GGWCC 1 cut(s) 487
SmlI CTYRAG 4 cut(s) 209, 458, 469, 560
SmoI CTYRAG 4 cut(s) 209, 458, 469, 560
SphI GCATGC 1 cut(s) 51
Sse9I AATT 2 cut(s) 18, 69
SsiI CCGC 1 cut(s) 44
SspMI CTAG 1 cut(s) 32
SstI GAGCTC 2 cut(s) 336, 585
StyD4I CCNGG 3 cut(s) 234, 270, 523
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 15
TaqI TCGA 1 cut(s) 336
TasI AATT 2 cut(s) 18, 69
TauI GCSGC 1 cut(s) 47
Tru1I TTAA 3 cut(s) 327, 492, 519
Tru9I TTAA 3 cut(s) 327, 492, 519
TscAI CASTG 1 cut(s) 559
TseFI GTSAC 1 cut(s) 550
Tsp45I GTSAC 1 cut(s) 550
TspDTI ATGAA 1 cut(s) 137
TspGWI ACGGA 1 cut(s) 375
TspRI CASTG 1 cut(s) 559
VpaK11BI GGWCC 1 cut(s) 487
XagI CCTNNNNNAGG 1 cut(s) 494
XceI RCATGY 4 cut(s) 51, 358, 427, 571
XmnI GAANNNNTTC 2 cut(s) 144, 287
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.